HG10003143 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003143
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionEncodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
LocationChr11: 17945388 .. 17946491 (+)
RNA-Seq ExpressionHG10003143
SyntenyHG10003143
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATGCCTTATTTGTTTCTCATTCTTTTAATCTTCTTGTCTACTTTGCATGTGTGCGACGCTCGTCGTTTTCGATTCTTTGAGAGTTCGAGTTCTGTTTCGAGGATTGAACCGCATTTGATATCCCAGGGTATTTGGACGGATCAGACGGAAAGAAGTTTTGATGAAAGCAATAGTGACAAGGCTTCTTATAAAGAGATTATTGTTGGGAATGAAATGTTAAAGGTATATTACATATATATTCCATGTCTCATATCTTTATGATTTAGTTCATTTTTTAAAATAAAGAATACGTAGGATCAGGGGTACACTCGAATATCTCCACTAGGTGGACACTCTCTTAACACCTACATCATCTCCCAATTAAATATATTCGATATCTATATGGGAGGGAGAGGGGAGTGTCAAGAAACCATATGAACTAAAATATTTTTTATAGATATGTAGACATTTTCATTGACATTTCTATATGTTGGAAAGAAGCTCAACATCAAAATATACAAGGTGACAATAAAATATATATTTCCCTCATAAATAATGTTATAAACATGTCAACAAACAAAAAATATAAATAAACAAATACAATTGTATAATGATCAAGGACTTATCTTGTTTGGAAATTTCAACCATTTCCATGGTTTTTGGTCAAAATATTTATCGGTATCAATATATTCGTTTTATACAGACCAATGTAATATCATTTTATCGTCATTTCTATTTACATTTGTATAAACTTGAAATCTCATAATTTCCATCAACATCAACATTTAATTTTTGATTTTTGAGTCACAAGACAAATCACGAATATAGACAACATTTAATTTCTTGGATCAAGACGAGTTTATTAATATAACATAGACAAAGGCACATGAATGATAAATGAATGAAAAAATGGATTATGGAAATATAGTGAGTGATATTAATTATGAACATTGGTATTTTTAGCAGGGAATGAAGAAGCATGGGAAGAGGTTGGTAATGGAGTTTCCAAAGAAAGAAAAAGTGTTGAATTCTGAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGAAAACCTCCTATTCACAATGCAAAACCCTAA

mRNA sequence

ATGGCCATGCCTTATTTGTTTCTCATTCTTTTAATCTTCTTGTCTACTTTGCATGTGTGCGACGCTCGTCGTTTTCGATTCTTTGAGAGTTCGAGTTCTGTTTCGAGGATTGAACCGCATTTGATATCCCAGGGTATTTGGACGGATCAGACGGAAAGAAGTTTTGATGAAAGCAATAGTGACAAGGCTTCTTATAAAGAGATTATTGTTGGGAATGAAATGTTAAAGGGAATGAAGAAGCATGGGAAGAGGTTGGTAATGGAGTTTCCAAAGAAAGAAAAAGTGTTGAATTCTGAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGAAAACCTCCTATTCACAATGCAAAACCCTAA

Coding sequence (CDS)

ATGGCCATGCCTTATTTGTTTCTCATTCTTTTAATCTTCTTGTCTACTTTGCATGTGTGCGACGCTCGTCGTTTTCGATTCTTTGAGAGTTCGAGTTCTGTTTCGAGGATTGAACCGCATTTGATATCCCAGGGTATTTGGACGGATCAGACGGAAAGAAGTTTTGATGAAAGCAATAGTGACAAGGCTTCTTATAAAGAGATTATTGTTGGGAATGAAATGTTAAAGGGAATGAAGAAGCATGGGAAGAGGTTGGTAATGGAGTTTCCAAAGAAAGAAAAAGTGTTGAATTCTGAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGAAAACCTCCTATTCACAATGCAAAACCCTAA

Protein sequence

MAMPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESNSDKASYKEIIVGNEMLKGMKKHGKRLVMEFPKKEKVLNSEENEVGEDMVVMDYAQPHRKPPIHNAKP
Homology
BLAST of HG10003143 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 211.8 bits (538), Expect = 3.3e-51
Identity = 108/124 (87.10%), Postives = 111/124 (89.52%), Query Frame = 0

Query: 3   MPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESNSDK 62
           MPYLFLILLIF STLH C+AR  RFFESSS VSRIEPH ISQGIW DQ ERSF+ESNSDK
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRIEPHSISQGIWADQPERSFEESNSDK 60

Query: 63  ASYKEIIVGNEMLKGMKKHGKRLVMEFPKKEKVLNSEENEVGEDMVVMDYAQPHRKPPIH 122
            SYKEIIVG EMLKGMKKHG RLVMEFP KEKVLNS+ENEVGEDMVVMDYAQPHRKPPIH
Sbjct: 61  LSYKEIIVGIEMLKGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHRKPPIH 120

Query: 123 NAKP 127
           N KP
Sbjct: 121 NEKP 124

BLAST of HG10003143 vs. NCBI nr
Match: XP_038905996.1 (uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida])

HSP 1 Score: 207.2 bits (526), Expect = 8.2e-50
Identity = 108/125 (86.40%), Postives = 111/125 (88.80%), Query Frame = 0

Query: 3   MPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESNSDK 62
           MPYLFLILLIF STLH C+AR  RFFESSS VSRIEPH ISQGIW DQ ERSF+ESNSDK
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRIEPHSISQGIWADQPERSFEESNSDK 60

Query: 63  ASYKEIIVGNEMLK-GMKKHGKRLVMEFPKKEKVLNSEENEVGEDMVVMDYAQPHRKPPI 122
            SYKEIIVG EMLK GMKKHG RLVMEFP KEKVLNS+ENEVGEDMVVMDYAQPHRKPPI
Sbjct: 61  LSYKEIIVGIEMLKQGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHRKPPI 120

Query: 123 HNAKP 127
           HN KP
Sbjct: 121 HNEKP 125

BLAST of HG10003143 vs. NCBI nr
Match: XP_011650407.1 (uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical protein Csa_010787 [Cucumis sativus])

HSP 1 Score: 169.1 bits (427), Expect = 2.5e-38
Identity = 92/130 (70.77%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRI-EPHLISQGIWT--DQTERSFDE 60
           MAMPY FLILL F STLH CDA R RFF+SS+ VSRI E H +SQGIW+     E SFD+
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  SNSDKASY-KEIIVGNEMLKGMKKHGKRLVMEFPKKEKVLNSEENEVGEDMVVMDYAQPH 120
           +N+D   + KE I+G EMLKGMK+ G RLVMEFP KEKV+NS+ENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNAKP 127
           RKPPIHN KP
Sbjct: 121 RKPPIHNEKP 130

BLAST of HG10003143 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 121.7 bits (304), Expect = 4.5e-24
Identity = 79/135 (58.52%), Postives = 90/135 (66.67%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLST-LHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESN 60
           M MP  F + L+FLST LH CDAR  +   S    SR+E  LIS G  T+Q ERS DES 
Sbjct: 1   MPMPTSFFLALLFLSTFLHACDARLLKLCHS----SRLESCLISPGFRTNQLERSLDESK 60

Query: 61  SDKASYKEIIVGNEMLKGMKKHGKRLVMEFPKK--------EKVLNSEENEVGEDMVVMD 120
           S+  S  E  VG E+LKGMKK  +RL ME P +        +KV+NSEENEVGEDMVVMD
Sbjct: 61  SNDVSSME-NVGIEILKGMKKL-RRLAMELPVRDRDRDKDIDKVVNSEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNAKP 127
           YAQPHRKPPIHN KP
Sbjct: 121 YAQPHRKPPIHNQKP 129

BLAST of HG10003143 vs. NCBI nr
Match: KAG7028383.1 (Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-23
Identity = 77/135 (57.04%), Postives = 90/135 (66.67%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLST-LHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESN 60
           M MP    + L+FLST LH CDAR  +   S    SR+EP +IS G  T+Q E+S DES 
Sbjct: 1   MPMPTSLFLSLLFLSTFLHACDARLLKLCHS----SRLEPCMISPGFRTNQLEKSLDESR 60

Query: 61  SDKASYKEIIVGNEMLKGMKKHGKRLVMEFPKK--------EKVLNSEENEVGEDMVVMD 120
           S+  S  E  VG E+LKGMKK  +RL ME P +        +KV+NSEENEVGEDMVVMD
Sbjct: 61  SNDVSSME-NVGIEILKGMKKL-RRLAMELPVRDIDRDKDIDKVVNSEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNAKP 127
           YAQPHRKPPIHN KP
Sbjct: 121 YAQPHRKPPIHNQKP 129

BLAST of HG10003143 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.9e-04
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 0

Query: 102 EVGEDMVVMDYAQPHRKPPIHNAK 126
           E GE++VVMDY QPHRKPPIHN K
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

BLAST of HG10003143 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.2e-38
Identity = 92/130 (70.77%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRI-EPHLISQGIWT--DQTERSFDE 60
           MAMPY FLILL F STLH CDA R RFF+SS+ VSRI E H +SQGIW+     E SFD+
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  SNSDKASY-KEIIVGNEMLKGMKKHGKRLVMEFPKKEKVLNSEENEVGEDMVVMDYAQPH 120
           +N+D   + KE I+G EMLKGMK+ G RLVMEFP KEKV+NS+ENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNAKP 127
           RKPPIHN KP
Sbjct: 121 RKPPIHNEKP 130

BLAST of HG10003143 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.7e-22
Identity = 75/137 (54.74%), Postives = 89/137 (64.96%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLST-LHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTERSFDESN 60
           M MP    + L+FLST LH CDAR  +   S    SR+E  +IS G   DQ +RS D+S 
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHS----SRLESCMISPGFRMDQLKRSLDKSK 60

Query: 61  SDKASYKEIIVGNEMLKGMKKHGKRLVMEFPKK----------EKVLNSEENEVGEDMVV 120
           S++ S  E  VG E+LKGMKK  +RL ME P +          +KV+NSEENEVGEDMVV
Sbjct: 61  SNEVSSVE-NVGIEILKGMKKL-RRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVV 120

Query: 121 MDYAQPHRKPPIHNAKP 127
           MDYAQPHRKPPIHN KP
Sbjct: 121 MDYAQPHRKPPIHNRKP 131

BLAST of HG10003143 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.6e-22
Identity = 76/130 (58.46%), Postives = 89/130 (68.46%), Query Frame = 0

Query: 1   MAMPYLFLILLIFLSTLHVCDA-RRFRFFESSSSVSRIEPHL-ISQGIWTDQTERSFDES 60
           MA P L  ILL  LSTLH CDA  R + FE S+ VSR+     I Q   TDQ ERS +++
Sbjct: 1   MATPSLLFILL--LSTLHACDACHRLQLFEYSNPVSRLGLLAPIPQEFRTDQPERSLNKN 60

Query: 61  NSDKASYKEIIVGNEMLKGMKKHGKRLVMEF--PKKEKVLNSEENEVGEDMVVMDYAQPH 120
              K S   + +G  +LKGMKKH KRL MEF   +KEK+++ EENEVGEDMVVMDYAQPH
Sbjct: 61  IIHKVS-SSVEIGIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNAKP 127
           RKPPIHN KP
Sbjct: 121 RKPPIHNKKP 126

BLAST of HG10003143 vs. ExPASy TrEMBL
Match: A0A438K320 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-06
Identity = 46/127 (36.22%), Postives = 69/127 (54.33%), Query Frame = 0

Query: 3   MPYLFLILLIFLSTLHVCDARRFRFFESSSSVSRIEPHLISQGI-WTDQTER-SFDESNS 62
           M  L L+LL+ LS +H C+AR        +     E HL+S+ +   DQ  + S      
Sbjct: 1   MSSLLLLLLLSLS-MHACNARHLGVIGKENR----EVHLLSKVVEEADQVHKISLPPRLI 60

Query: 63  DKASYKEIIVGNEMLKGMKKHGKRLVMEFPKK-EKVLNSEENEVGEDMVVMDYAQPHRKP 122
                 E +   ++L G K+H + ++   P   E+ ++S+E ++ ED+VVMDYAQPHRKP
Sbjct: 61  PSMETAEKLQPQQILPGWKRHSRLMLGSAPHDVEETVDSKEGDIVEDIVVMDYAQPHRKP 120

Query: 123 PIHNAKP 127
           PIHN  P
Sbjct: 121 PIHNKDP 122

BLAST of HG10003143 vs. ExPASy TrEMBL
Match: A0A2G9HFQ2 (Uncharacterized protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10996 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.7e-06
Identity = 46/136 (33.82%), Postives = 67/136 (49.26%), Query Frame = 0

Query: 8   LILLIFLSTLHVCDARRFRFFESSSSVSRIEPHLISQGIWTDQTER------SFDESNSD 67
           L  L+    LH C AR    ++  S+V+++E  L S+ + T   ER       F    S+
Sbjct: 4   LFYLVLYLYLHACAARPLLVWDDGSTVTQLE--LSSKDVRTSVAERLIITDVKFRAEQSE 63

Query: 68  KAS-------------YKEIIVGNEMLKG-MKKHGKRLVMEFPKKEKVLNSEENEVGEDM 124
           K++                    +E +KG  KK   RL +E P  E+ +NS++N   +D+
Sbjct: 64  KSTLDLGTNIVQLDNHMSSATKEDENIKGNFKKRADRLTLESP--EETMNSKDNNTMDDI 123

BLAST of HG10003143 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 47.0 bits (110), Expect = 1.3e-05
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 0

Query: 102 EVGEDMVVMDYAQPHRKPPIHNAK 126
           E GE++VVMDY QPHRKPPIHN K
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905997.13.3e-5187.10uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida][more]
XP_038905996.18.2e-5086.40uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida][more]
XP_011650407.12.5e-3870.77uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical ... [more]
XP_023540680.14.5e-2458.52uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo][more]
KAG7028383.11.3e-2357.04Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q93VK81.9e-0479.17Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L2981.2e-3870.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1[more]
A0A6J1KZM92.7e-2254.74uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521... [more]
A0A6J1DKL14.6e-2258.46uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A438K3201.2e-0636.22Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1[more]
A0A2G9HFQ22.7e-0633.82Uncharacterized protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10996 P... [more]
Match NameE-valueIdentityDescription
AT4G16515.11.3e-0579.17Encodes a root meristem growth factor (RGF). Belongs to a family of functionall... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34961TRANSMEMBRANE PROTEINcoord: 62..126
NoneNo IPR availablePANTHERPTHR34961:SF7SUBFAMILY NOT NAMEDcoord: 62..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003143.1HG10003143.1mRNA