HG10002641 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10002641
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RNase H domain-containing protein
Location	Chr11: 9301633 .. 9302070 (+)
RNA-Seq Expression	HG10002641
Synteny	HG10002641

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTTGAACATGGAGCTGCTTGGAGTCCTGATCATCGTTCTAGTGGCCTTGGTTGGGTGCTTCGGAACCACTTAGGTAGAGTTCGTCTTGTTGGTCTCAAGTTTATCAAAAGATGCAATGATGTAAAGGTCCTTGAAGCTCTGGCTATCTGTGAAGGCCATCTAAGCATTATTTCTCACAATATCCGACCAATTGTTGTGGAATCAAAGTGTTTGGAGGTTGTTAACCTCTTGTCCGGATGCTCTAAGGATCTTTCAGAAGTTTCCTTCTTCATCCACGAGGCTAAGTTGTATGCTCAAGAGTTAGGCGATGTTTGTTTTGCCCATGTTCGTTGCTGGAATAATGTTTTAGCCCACTCTTTTGCCTCAAAGGCGTTGGGAGAGCAATTTCTTCATTTCTTCTTTCTCCCTACCTTGAGTGGTTTGGGTCTCTAG

mRNA sequence

ATGGAAGTTGAACATGGAGCTGCTTGGAGTCCTGATCATCGTTCTAGTGGCCTTGGTTGGGTGCTTCGGAACCACTTAGGTAGAGTTCGTCTTGTTGGTCTCAAGTTTATCAAAAGATGCAATGATGTAAAGGTCCTTGAAGCTCTGGCTATCTGTGAAGGCCATCTAAGCATTATTTCTCACAATATCCGACCAATTGTTGTGGAATCAAAGTGTTTGGAGGTTGTTAACCTCTTGTCCGGATGCTCTAAGGATCTTTCAGAAGTTTCCTTCTTCATCCACGAGGCTAAGTTGTATGCTCAAGAGTTAGGCGATGTTTGTTTTGCCCATGTTCGTTGCTGGAATAATGTTTTAGCCCACTCTTTTGCCTCAAAGGCGTTGGGAGAGCAATTTCTTCATTTCTTCTTTCTCCCTACCTTGAGTGGTTTGGGTCTCTAG

Coding sequence (CDS)

ATGGAAGTTGAACATGGAGCTGCTTGGAGTCCTGATCATCGTTCTAGTGGCCTTGGTTGGGTGCTTCGGAACCACTTAGGTAGAGTTCGTCTTGTTGGTCTCAAGTTTATCAAAAGATGCAATGATGTAAAGGTCCTTGAAGCTCTGGCTATCTGTGAAGGCCATCTAAGCATTATTTCTCACAATATCCGACCAATTGTTGTGGAATCAAAGTGTTTGGAGGTTGTTAACCTCTTGTCCGGATGCTCTAAGGATCTTTCAGAAGTTTCCTTCTTCATCCACGAGGCTAAGTTGTATGCTCAAGAGTTAGGCGATGTTTGTTTTGCCCATGTTCGTTGCTGGAATAATGTTTTAGCCCACTCTTTTGCCTCAAAGGCGTTGGGAGAGCAATTTCTTCATTTCTTCTTTCTCCCTACCTTGAGTGGTTTGGGTCTCTAG

Protein sequence

MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASKALGEQFLHFFFLPTLSGLGL

Homology

[Close]

BLAST of HG10002641 vs. NCBI nr
Match: XP_038886170.1 (uncharacterized protein LOC120076417 [Benincasa hispida])

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-18
Identity = 58/115 (50.43%), Postives = 75/115 (65.22%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNIRPI 66
           A+W+    + GLGWV  +HL RV + GLKF+ RC  V +LEA+AIC G   + S +I  I
Sbjct: 83  ASWNSKIDACGLGWVFLDHLYRVCMAGLKFVLRCQKVNILEAIAICFGLEILSSIDISNI 142

Query: 67  VVESKCL-EVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 121
           +VES CL EV+NLL+    DLSEVSF   EAK     LG + F+HVR + NVLA+
Sbjct: 143 MVESDCLEEVINLLNDDVVDLSEVSFCSEEAKDRGGNLGVISFSHVRRYRNVLAY 197

[Close]

BLAST of HG10002641 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-10
Identity = 39/121 (32.23%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNIRPI 66
           AAW  D  + G+GW+LR+  G V     + I+   ++  LE +AICEG  +I   + RPI
Sbjct: 93  AAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRPI 152

Query: 67  VVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASKA 126
            +ES  LE ++LL    +D +E+ + + E     +++  V   H+    N +AH  A +A
Sbjct: 153 HLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

Query: 127 L 128
           +
Sbjct: 213 M 213

[Close]

BLAST of HG10002641 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 75.1 bits (183), Expect = 5.6e-10
Identity = 41/121 (33.88%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNI-RP 66
           A+WS      G+GW++R+  G + L G +F++ CN+VK+LEA AI EG  ++ +  + RP
Sbjct: 85  ASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRP 144

Query: 67  IVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASK 126
           + +E+   EV +LL+   +DL++  + + E          + FA V    N  AHS A +
Sbjct: 145 LHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQR 204

[Close]

BLAST of HG10002641 vs. NCBI nr
Match: BAS77245.1 (Os02g0175302 [Oryza sativa Japonica Group])

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-09
Identity = 43/135 (31.85%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIIS 60
           M++    ++ PD    GLG VLRN LG V      +I+RC      E +A  EG L  + 
Sbjct: 1   MKLNIDGSFQPDTGKGGLGVVLRNRLGEVIFAACGYIQRCTGALEAELMACREGLLMALQ 60

Query: 61  HNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 120
             + PI++E+ C E++NLL       SE+ F + E          V  A V    N ++H
Sbjct: 61  WTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKEVHDLMMGNRVVSLAKVHRNQNRISH 120

Query: 121 SFASKALGEQFLHFF 136
             A+K   E+  HF+
Sbjct: 121 VLANKGRVEELTHFW 135

[Close]

BLAST of HG10002641 vs. NCBI nr
Match: EEE56417.1 (hypothetical protein OsJ_05582 [Oryza sativa Japonica Group])

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-09
Identity = 43/135 (31.85%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIIS 60
           M++    ++ PD    GLG VLRN LG V      +I+RC      E +A  EG L  + 
Sbjct: 112 MKLNIDGSFQPDTGKGGLGVVLRNRLGEVIFAACGYIQRCTGALEAELMACREGLLMALQ 171

Query: 61  HNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 120
             + PI++E+ C E++NLL       SE+ F + E          V  A V    N ++H
Sbjct: 172 WTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKEVHDLMMGNRVVSLAKVHRNQNRISH 231

Query: 121 SFASKALGEQFLHFF 136
             A+K   E+  HF+
Sbjct: 232 VLANKGRVEELTHFW 246

[Close]

BLAST of HG10002641 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 9.3e-11
Identity = 39/121 (32.23%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNIRPI 66
           AAW  D  + G+GW+LR+  G V     + I+   ++  LE +AICEG  +I   + RPI
Sbjct: 93  AAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQEHCRPI 152

Query: 67  VVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASKA 126
            +ES  LE ++LL    +D +E+ + + E     +++  V   H+    N +AH  A +A
Sbjct: 153 HLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

Query: 127 L 128
           +
Sbjct: 213 M 213

[Close]

BLAST of HG10002641 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.7e-10
Identity = 41/121 (33.88%), Postives = 68/121 (56.20%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNI-RP 66
           A+WS      G+GW++R+  G + L G +F++ CN+VK+LEA AI EG  ++ +  + RP
Sbjct: 85  ASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLGVLRP 144

Query: 67  IVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASK 126
           + +E+   EV +LL+   +DL++  + + E          + FA V    N  AHS A +
Sbjct: 145 LHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCAHSLAQR 204

[Close]

BLAST of HG10002641 vs. ExPASy TrEMBL
Match: B8AHI8 (Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_07152 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-09
Identity = 43/135 (31.85%), Postives = 63/135 (46.67%), Query Frame = 0


Query: 1   MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIIS 60
           M++    ++ P     G+G VLR   GRV      FI RCN     E +A  +G    + 
Sbjct: 484 MKLNIDGSYGPQDGRGGIGAVLRESFGRVIFSACGFIGRCNGALESELIACRDGLNLALQ 543

Query: 61  HNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 120
             + PI VES CLE++ LL+   K LS   F + E +       +V FA V    N ++H
Sbjct: 544 WTLLPIAVESDCLEIIQLLNSKEKLLSAEGFIVREIRFLMTGTKEVSFAKVHRNQNRVSH 603

Query: 121 SFASKALGEQFLHFF 136
             A+K   E  + F+
Sbjct: 604 FLANKGRCESMMEFW 618

[Close]

BLAST of HG10002641 vs. ExPASy TrEMBL
Match: B9F3B9 (RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_05582 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-09
Identity = 43/135 (31.85%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIIS 60
           M++    ++ PD    GLG VLRN LG V      +I+RC      E +A  EG L  + 
Sbjct: 112 MKLNIDGSFQPDTGKGGLGVVLRNRLGEVIFAACGYIQRCTGALEAELMACREGLLMALQ 171

Query: 61  HNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 120
             + PI++E+ C E++NLL       SE+ F + E          V  A V    N ++H
Sbjct: 172 WTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKEVHDLMMGNRVVSLAKVHRNQNRISH 231

Query: 121 SFASKALGEQFLHFF 136
             A+K   E+  HF+
Sbjct: 232 VLANKGRVEELTHFW 246

[Close]

BLAST of HG10002641 vs. ExPASy TrEMBL
Match: A0A0P0VFG5 (Os02g0175302 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0175302 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.3e-09
Identity = 43/135 (31.85%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   MEVEHGAAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIIS 60
           M++    ++ PD    GLG VLRN LG V      +I+RC      E +A  EG L  + 
Sbjct: 1   MKLNIDGSFQPDTGKGGLGVVLRNRLGEVIFAACGYIQRCTGALEAELMACREGLLMALQ 60

Query: 61  HNIRPIVVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAH 120
             + PI++E+ C E++NLL       SE+ F + E          V  A V    N ++H
Sbjct: 61  WTLLPIIIETDCQEMLNLLQSKELVRSELMFLVKEVHDLMMGNRVVSLAKVHRNQNRISH 120

Query: 121 SFASKALGEQFLHFF 136
             A+K   E+  HF+
Sbjct: 121 VLANKGRVEELTHFW 135

[Close]

BLAST of HG10002641 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 5.7e-05
Identity = 31/128 (24.22%), Postives = 58/128 (45.31%), Query Frame = 0


Query: 7   AAWSPDHRSSGLGWVLRNHLGRVRLVGLKFIKRCNDVKVLEALAICEGHLSIISHNIRPI 66
           A W+ D+   G+GWVLRN  G V+ +G + + +   V   E  A+    LS+       +
Sbjct: 434 ATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYV 493

Query: 67  VVESKCLEVVNLLSGCSKDLSEVSFFIHEAKLYAQELGDVCFAHVRCWNNVLAHSFASKA 126
           + ES    ++ +L+   +    +   I + +    +  +V F  +    N LA   A ++
Sbjct: 494 IFESDSQVLIEILNN-DEIWPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAERVARES 553

Query: 127 LGEQFLHF 135
           L   FL++
Sbjct: 554 L--SFLNY 558

The following BLAST results are available for this feature:

BLAST of HG10002641 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886170.1	1.5e-18	50.43	uncharacterized protein LOC120076417 [Benincasa hispida]	[more]
XP_022143535.1	1.9e-10	32.23	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022155262.1	5.6e-10	33.88	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
BAS77245.1	2.8e-09	31.85	Os02g0175302 [Oryza sativa Japonica Group]	[more]
EEE56417.1	2.8e-09	31.85	hypothetical protein OsJ_05582 [Oryza sativa Japonica Group]	[more]

BLAST of HG10002641 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of HG10002641 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	9.3e-11	32.23	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DNV9	2.7e-10	33.88	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
B8AHI8	1.3e-09	31.85	Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_07152 PE=4...	[more]
B9F3B9	1.3e-09	31.85	RNase H domain-containing protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os...	[more]
A0A0P0VFG5	1.3e-09	31.85	Os02g0175302 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0175302 PE...	[more]

BLAST of HG10002641 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	5.7e-05	24.22	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 7..126 e-value: 1.2E-16 score: 60.6
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 4..129 e-value: 1.5E-7 score: 33.5
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 8..125
None	No IPR available	PANTHER	PTHR33033:SF52	OS02G0175302 PROTEIN	coord: 8..125
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 7..123 e-value: 9.48878E-15 score: 64.2576
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 7..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10002641.1	HG10002641.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity