HG10002413 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002413
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectinesterase
LocationChr11: 6497191 .. 6499740 (+)
RNA-Seq ExpressionHG10002413
SyntenyHG10002413
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTTGGGTTGCATAAAAGATGACTACAACCAAGTTTTTTTTCTTTTTAAAAATTTAAAAAGAGATTTTTTTTTTTTTTTTTGGAAAAAAAAAAGAGAGAATAAAATAAAAAAGAAAAGATTCATCCATGAAAGAGAAGTTCACATCTGTGGTATTCTACTTTATTATGTTCCTTTTTATAAAGGCAATTCCCTGAGCAACCTTCATTTCTCGATCGCCACGCATTGCCCTTTCAATTTCCAAATCCTACACTCTCTCTCTTTTCTCTTCATTCTTCAATTTGCAGAACGGAACCCCAACTCTGAAATCCATGGATACCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGGCAAAAGACTCGCCGTCGGCTAATTATACTCCTTCTTTCCATTGTTCTTCTAATTGCTCTCATCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGAAACTCTTCTTCTTCATCGCCGACGAGCTCAGCTCCTCCCACCGAGATCACTCCTGCTGCCTCACTCAAAACTCTCTGTAGTGTCACTCAGTATCCAAGTTCGTGTCAGTCCTCTCTCCAAAACTCCAACACCACCGATCCGGTATTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACTGATTCGTTCTCGAAACTCTCCGATTATACCTCTAATCTCAATTCCAATACTGGAGATCCGAAGGTTAAAGCCGCAATTGAAATCTGCCGCAGTGTTTTTGAAGATGCCATTGACACGCTAAACGACACCGTTTCATCCATGGAAGTAGATCACCATAGTGAGAAGTTCTTGTCGCCGTCTAGGATTGAAGATCTTAAAACCTGGCTGAGCACTGCAATAACAGATCAAGAAACCTGCCTCGACGCCGTTCGAGATCTAAACCAGACGACGGTTCTCCAAAACTTGCAAACGGCAATGGCGAACTCTACCGAGTTTACTAGCAATAGTTTAGCGATTGTAACGAAAATTCTTGGATTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGGACTCCCGGAGTGGGTAAGCTCCGGCGACCGAAGGCTTTTGCAGGAGAACAATGTGACGGCGCATGTGACGGTGTCGAAGGATGGGAAAGGGGATTACACAACGATCAAAGATGCCGTAGCAGCGGTGCCAAAGAAGAGCAAAGAAAGATTCATCATATACGTAAAAGAAGGAATATACGAAGAGAATGTGATTTTAGACAAGAACAAGTGGAATGTTATGATGTATGGAGATGGTAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGGTAACCCCATTAATGATTTTCTCTTTCTGAATTGCTTCATTTAATCTTAGTTAAATTATACAGAGTACTCTTTTGAATTTTATCCATACCTTTCAATTATGTTCATAAACTTGCAAGTTTTACTCTAACCACTTCAAAATTGTTCTGAAAAGTATTTTTAGAAGAAATTTTCTAAGAAAAATGGGAAAGATACAAATTAGATCAGTATATTTTTGTATAGTTGATTTACTTTTGAACAATGCATCCATGGCTGCCATGTTGTTGTTCCTGTTCGTACCTATTTTGTTTGGCCTTTTTTGGGCCGAAGCCAAAACCGCTCAAAATTCAAGGACAATCAGTGTATTGAGTGGATAATGGGCCATTGCCCTTGTCGTCAGAAACCCACATGGTGGATGTGTCAGCCATGTGAAGGCAACTTTTCCCCACTTTCCCTTTTGACCTGTCATTTCTTCTTCTCTGTTCATGTAAATCGGCTATTTGTTGATTAATTTTGTTTGGTTTTGCAGCTGTTGCTGGGAAAGGGTTCATTGGAAAGGACATGGGGTTCATCAACACGGCCGGTCCAGCCAAGCACCAGGCTGTTGCCTTCCGGTCAGGCTCTGACCTTTCTGTGATGTCCGGATGTTCATTTGATGGCTATCAAGACACTCTCTACGCCCATTCAAACCGTCAATTCTATCGAGACTGCGACATCACTGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTGGTGTTCCAGAATTGCAAAATCCGGCCAAGGCAGCCACTTCTCAACCAATTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCGGCCTACAACGAAAGTCTCACCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTTTCCACCACGGTGATTATGCAGTCGGAGATCGGAGCATTCCTGCAGCCCGTCGGGTGGAAGGAATGGGTCAGCGGGCAAGACCCACCCAACACCATATTTTATGGGGAGTATCAGAATACAGGGCCTGGATCCAGCGTGGACAAGAGGGTTAAATGGGTCGGTTACAGGTCCAGTCTTACAGACGCCGAGGCCGGAAAGTTCACTGTCAGGACCTTTCTCAACGGCGAGGATTGGTTGCCGGCCACCAGTGTAAAGTTTGATACATCCTTGTGA

mRNA sequence

ATGTCGTTGGGTTGCATAAAAGATGACTACAACCAAAACGGAACCCCAACTCTGAAATCCATGGATACCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGGCAAAAGACTCGCCGTCGGCTAATTATACTCCTTCTTTCCATTGTTCTTCTAATTGCTCTCATCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGAAACTCTTCTTCTTCATCGCCGACGAGCTCAGCTCCTCCCACCGAGATCACTCCTGCTGCCTCACTCAAAACTCTCTGTAGTGTCACTCAGTATCCAAGTTCGTGTCAGTCCTCTCTCCAAAACTCCAACACCACCGATCCGGTATTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACTGATTCGTTCTCGAAACTCTCCGATTATACCTCTAATCTCAATTCCAATACTGGAGATCCGAAGGTTAAAGCCGCAATTGAAATCTGCCGCAGTGTTTTTGAAGATGCCATTGACACGCTAAACGACACCGTTTCATCCATGGAAGTAGATCACCATAGTGAGAAGTTCTTGTCGCCGTCTAGGATTGAAGATCTTAAAACCTGGCTGAGCACTGCAATAACAGATCAAGAAACCTGCCTCGACGCCGTTCGAGATCTAAACCAGACGACGGTTCTCCAAAACTTGCAAACGGCAATGGCGAACTCTACCGAGTTTACTAGCAATAGTTTAGCGATTGTAACGAAAATTCTTGGATTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGGACTCCCGGAGTGGGTAAGCTCCGGCGACCGAAGGCTTTTGCAGGAGAACAATGTGACGGCGCATGTGACGGTGTCGAAGGATGGGAAAGGGGATTACACAACGATCAAAGATGCCGTAGCAGCGGTGCCAAAGAAGAGCAAAGAAAGATTCATCATATACGTAAAAGAAGGAATATACGAAGAGAATGTGATTTTAGACAAGAACAAGTGGAATGTTATGATGTATGGAGATGGTAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTTGCTGGGAAAGGGTTCATTGGAAAGGACATGGGGTTCATCAACACGGCCGGTCCAGCCAAGCACCAGGCTGTTGCCTTCCGGTCAGGCTCTGACCTTTCTGTGATGTCCGGATGTTCATTTGATGGCTATCAAGACACTCTCTACGCCCATTCAAACCGTCAATTCTATCGAGACTGCGACATCACTGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTGGTGTTCCAGAATTGCAAAATCCGGCCAAGGCAGCCACTTCTCAACCAATTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCGGCCTACAACGAAAGTCTCACCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTTTCCACCACGGTGATTATGCAGTCGGAGATCGGAGCATTCCTGCAGCCCGTCGGGTGGAAGGAATGGGTCAGCGGGCAAGACCCACCCAACACCATATTTTATGGGGAGTATCAGAATACAGGGCCTGGATCCAGCGTGGACAAGAGGGTTAAATGGGTCGGTTACAGGTCCAGTCTTACAGACGCCGAGGCCGGAAAGTTCACTGTCAGGACCTTTCTCAACGGCGAGGATTGGTTGCCGGCCACCAGTGTAAAGTTTGATACATCCTTGTGA

Coding sequence (CDS)

ATGTCGTTGGGTTGCATAAAAGATGACTACAACCAAAACGGAACCCCAACTCTGAAATCCATGGATACCGTCAAGTCCTTCAAGGGCTACGGCAAGGTCGACGAGCTCGAACAGCAAGCTTTCCGGCAAAAGACTCGCCGTCGGCTAATTATACTCCTTCTTTCCATTGTTCTTCTAATTGCTCTCATCATCGGCGCCGTCGTCGGAATCGTCATTCATAAACGAAACTCTTCTTCTTCATCGCCGACGAGCTCAGCTCCTCCCACCGAGATCACTCCTGCTGCCTCACTCAAAACTCTCTGTAGTGTCACTCAGTATCCAAGTTCGTGTCAGTCCTCTCTCCAAAACTCCAACACCACCGATCCGGTATTTCTCTTCAAGCTTTCTCTCCGAGTCGCCACTGATTCGTTCTCGAAACTCTCCGATTATACCTCTAATCTCAATTCCAATACTGGAGATCCGAAGGTTAAAGCCGCAATTGAAATCTGCCGCAGTGTTTTTGAAGATGCCATTGACACGCTAAACGACACCGTTTCATCCATGGAAGTAGATCACCATAGTGAGAAGTTCTTGTCGCCGTCTAGGATTGAAGATCTTAAAACCTGGCTGAGCACTGCAATAACAGATCAAGAAACCTGCCTCGACGCCGTTCGAGATCTAAACCAGACGACGGTTCTCCAAAACTTGCAAACGGCAATGGCGAACTCTACCGAGTTTACTAGCAATAGTTTAGCGATTGTAACGAAAATTCTTGGATTGTTGGCGGATTTCAACATCCCTATCCACCGGAAACTGATGGGACTCCCGGAGTGGGTAAGCTCCGGCGACCGAAGGCTTTTGCAGGAGAACAATGTGACGGCGCATGTGACGGTGTCGAAGGATGGGAAAGGGGATTACACAACGATCAAAGATGCCGTAGCAGCGGTGCCAAAGAAGAGCAAAGAAAGATTCATCATATACGTAAAAGAAGGAATATACGAAGAGAATGTGATTTTAGACAAGAACAAGTGGAATGTTATGATGTATGGAGATGGTAGAACCAAAACCATTGTTTCCGGCCGCCTGAACTTCATCGACGGCACTCCCACCTTCTCCACCGCCACTTTTGCTGTTGCTGGGAAAGGGTTCATTGGAAAGGACATGGGGTTCATCAACACGGCCGGTCCAGCCAAGCACCAGGCTGTTGCCTTCCGGTCAGGCTCTGACCTTTCTGTGATGTCCGGATGTTCATTTGATGGCTATCAAGACACTCTCTACGCCCATTCAAACCGTCAATTCTATCGAGACTGCGACATCACTGGCACCATCGACTTCATCTTCGGCAACGCCGCTGTGGTGTTCCAGAATTGCAAAATCCGGCCAAGGCAGCCACTTCTCAACCAATTCAACACCATCACAGCTCAAGGCAAAAAGGACATCAACCAAAACTCCGGCATTTCCATTCAGAAATGCACTTTTTCGGCCTACAACGAAAGTCTCACCGCCCCCACTTACTTGGGGCGGCCATGGAAGGAGTTTTCCACCACGGTGATTATGCAGTCGGAGATCGGAGCATTCCTGCAGCCCGTCGGGTGGAAGGAATGGGTCAGCGGGCAAGACCCACCCAACACCATATTTTATGGGGAGTATCAGAATACAGGGCCTGGATCCAGCGTGGACAAGAGGGTTAAATGGGTCGGTTACAGGTCCAGTCTTACAGACGCCGAGGCCGGAAAGTTCACTGTCAGGACCTTTCTCAACGGCGAGGATTGGTTGCCGGCCACCAGTGTAAAGTTTGATACATCCTTGTGA

Protein sequence

MSLGCIKDDYNQNGTPTLKSMDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSSSPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKTWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIPIHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL
Homology
BLAST of HG10002413 vs. NCBI nr
Match: XP_038877946.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 1069.7 bits (2765), Expect = 9.2e-309
Identity = 538/576 (93.40%), Postives = 559/576 (97.05%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILL+SIVLLIAL+I AVVGIVIHKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLVSIVLLIALVIAAVVGIVIHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S TSS PPTE+TP ASLKTLCSVTQYPSSCQSSL+NSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  SATSSPPPTELTPLASLKTLCSVTQYPSSCQSSLENSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSNLNSNT DPK+KAAIEICRSVFEDAIDTLNDTVSSMEVD H+EKFLSPSRI+DLK
Sbjct: 121 SDYTSNLNSNTEDPKLKAAIEICRSVFEDAIDTLNDTVSSMEVDRHNEKFLSPSRIDDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKIL LLADF+IP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILRLLADFDIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMG P+WVSSGDRRLLQENNVTAHVTVSKDGKG+YTTIKDAVAAVPKKSK+RFIIY
Sbjct: 241 IHRKLMGFPDWVSSGDRRLLQENNVTAHVTVSKDGKGNYTTIKDAVAAVPKKSKQRFIIY 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEG+YEENVILDK+KWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSV+SGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVISGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN++L APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDNLAAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FLQPVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           YR SLTDAEAGKFTV TFLNGEDWLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAGKFTVGTFLNGEDWLPATSVKFDTSL 576

BLAST of HG10002413 vs. NCBI nr
Match: XP_008460354.1 (PREDICTED: pectinesterase 3 [Cucumis melo])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.5e-308
Identity = 534/576 (92.71%), Postives = 558/576 (96.88%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NSNTGDPKVKAAI+IC+SVFEDAIDTLNDT+SSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ+LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAGKFTV TFLNGEDWLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of HG10002413 vs. NCBI nr
Match: KAA0053577.1 (pectinesterase 3 [Cucumis melo var. makuwa] >TYK19167.1 pectinesterase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.0e-307
Identity = 533/576 (92.53%), Postives = 557/576 (96.70%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NSNTGDPKVKAAI+IC+SVFEDAIDTLNDT+SSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDR+LLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAGKFTV TFLNGEDWLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of HG10002413 vs. NCBI nr
Match: XP_004144445.1 (pectinesterase 3 [Cucumis sativus] >KGN58475.1 hypothetical protein Csa_001265 [Cucumis sativus])

HSP 1 Score: 1060.1 bits (2740), Expect = 7.2e-306
Identity = 529/576 (91.84%), Postives = 556/576 (96.53%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NS TGDPKV+AAI+ICRSVFEDAIDTLNDTVSSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ+LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIM+SEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAG+FTV TFLNGEDWLPAT+V FDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

BLAST of HG10002413 vs. NCBI nr
Match: XP_023528356.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.2e-300
Identity = 521/576 (90.45%), Postives = 548/576 (95.14%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MD+VKSFKGYGKVDELE QAFRQKTRRRLIILLLS+VLLIAL+IGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSVVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S TSS PPTE+ PAASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDY SNLNS+T D KVKAAI IC SVF+DAIDTLNDTVSSMEVD H EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQT VL+NLQ  MANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLM  PEW+S GDRRLLQENNVTAH TVSKDG GD+TTIK+AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTAHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEG+YEENVILDK+KWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN+SLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIGAFLQP+GWKEWVSGQDPP++IFYGEYQN+GPGS+V+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGAFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           YR SLTDAEA KFTVRTFLNGEDWLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDTSL 576

BLAST of HG10002413 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.2e-207
Identity = 369/586 (62.97%), Postives = 455/586 (77.65%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDT+KSFKGYGKV+ELEQQA+ +KTR+RLII+ +S ++LIA+II AV G+VIH RNS SS
Sbjct: 1   MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSC---QSSLQNSNTTDPVFLFKLSLRVATDSF 140
             + S P TE++PAASLK +C  T+YPSSC    SSL  SNTTDP  LFKLSLRVA D  
Sbjct: 61  PSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAID-- 120

Query: 141 SKLSDYTSNLNSNT-GDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRI 200
            +LS + S L +N   D +++ AI++C SVF DA+D LND++S++     + +  S + +
Sbjct: 121 -ELSSFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGT--VAGRIASSASV 180

Query: 201 EDLKTWLSTAITDQETCLDAVRDLNQTT---VLQNLQTAMANSTEFTSNSLAIVTKILGL 260
            +++TWLS A+TDQ+TCLDAV +LN T     LQ ++TAM NSTEF SNSLAIVTKILGL
Sbjct: 181 SNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 240

Query: 261 LADFNIPI-HRKLMGLPEWVSSGDRRLLQE--NNVTAHVTVSKDGKGDYTTIKDAVAAVP 320
           L+ F  PI HR+L+G PEW+ + +RRLL+E  N+ T    V+KDG G + TI +A+  V 
Sbjct: 241 LSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVK 300

Query: 321 KKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFA 380
           KKS+ERF +YVKEG Y EN+ LDKN WNVM+YGDG+ KT V G  NF+DGTPTF TATFA
Sbjct: 301 KKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFA 360

Query: 381 VAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDC 440
           V GKGFI KD+GF+N AG +KHQAVA RSGSD SV   CSFDG+QDTLYAHSNRQFYRDC
Sbjct: 361 VKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDC 420

Query: 441 DITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYN 500
           DITGTIDFIFGNAAVVFQ+CKI PRQPL NQFNTITAQGKKD NQN+GI IQK T + + 
Sbjct: 421 DITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFG 480

Query: 501 ESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGS 560
            +LTAPTYLGRPWK+FSTTVIMQS+IGA L PVGW  WV   +PP TIFY EYQN+GPG+
Sbjct: 481 NNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGA 540

Query: 561 SVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
            V +RVKW GY+ ++TD  A +FTV++F+ G +WLP  +V+FD++L
Sbjct: 541 DVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of HG10002413 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.2e-199
Identity = 359/590 (60.85%), Postives = 445/590 (75.42%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MD+V SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIA+II AVV  V+HK N + S
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHK-NKNES 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSS---LQNSNTTDPVFLFKLSLRVATDSF 140
           +P   +PP E+TP+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D  
Sbjct: 61  TP---SPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 141 SKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIE 200
             +SD    L+  T D ++K+A+ +C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 201 DLKTWLSTAITDQETCLDAVRDLNQ-------TTVLQNLQTAMANSTEFTSNSLAIVTKI 260
           DLKTWLS  +TD ETC D++ +L Q       +T+ QNL++AM+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 261 LGLLADFNIPIHRK----------LMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYT 320
           L  L+D  IPIHR+           +   +W     RRLLQ   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 321 TIKDAVAAVPKKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDG 380
           T+ +AVA VPKKS + F+IYVK G Y ENV++DK+KWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 381 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 440
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 441 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGIS 500
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ CKI PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 501 IQKCTFSAYNESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFY 560
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM++ IGA ++P GW  WVSG DPP +I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 561 GEYQNTGPGSSVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSV 591
           GEY+NTGPGS V +RVKW GY+  ++DAEA KFTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of HG10002413 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.6e-157
Identity = 292/527 (55.41%), Postives = 369/527 (70.02%), Query Frame = 0

Query: 72  IHKRNSSSSSPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQ----NSNTTDPVFLFK 131
           IH+R+   S     +P    TP++ L+T+C+VT YP+SC SS+     +  TTDP  LF+
Sbjct: 436 IHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFR 495

Query: 132 LSLRVATDSFSKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSM-EVDHH 191
           LSL+V  D  + +      L   T D  +K+A+ +C  VF+ A+D++NDT+SS+ EV   
Sbjct: 496 LSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISG 555

Query: 192 SEKFLSPSRIEDLKTWLSTAITDQETCLDAVRDLN-QTTVLQNLQTAMANSTEFTSNSLA 251
            +K L+ S I DL TWLS+A+TD  TC D + + N  + + Q L++AM NSTEFTSNSLA
Sbjct: 556 GKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLA 615

Query: 252 IVTKILGLLADFNIPIH-RKLM---GLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTT 311
           IV ++L   +   IP+  R+L+     P WV  G RRLLQ  N+T HVTV+ DG GD  T
Sbjct: 616 IVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVRT 675

Query: 312 IKDAVAAVPKKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGT 371
           + +AV  VPKK K  F+IYVK G Y ENV++ K+KWNV +YGDGR KTI+SG  N +DG 
Sbjct: 676 VNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGV 735

Query: 372 PTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAH 431
            TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFDGYQDTLY H
Sbjct: 736 RTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTH 795

Query: 432 SNRQFYRDCDITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISI 491
           SNRQ+YR+CD+TGT+DFIFG   VVFQ C IRPRQPL NQFNTITA+G ++ NQN+GISI
Sbjct: 796 SNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISI 855

Query: 492 QKCTFSAYNESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQD-PPNTIFY 551
            +CT S  N ++TA TYLGRPWK FS TVIMQS IG+F+ P GW  W S  D PP TIFY
Sbjct: 856 HQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFY 915

Query: 552 GEYQNTGPGSSVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGED-WLP 587
            EY+N+GPGS + KRVKW GY+   +D EA +FTV+ FL G+D W+P
Sbjct: 916 REYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959


HSP 2 Score: 207.2 bits (526), Expect = 5.1e-52
Identity = 125/253 (49.41%), Postives = 172/253 (67.98%), Query Frame = 0

Query: 21  MDTVKSF-KGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSS 80
           MDTVKS  KGYGKVDE +  A ++KTR+RL  + +S+ +L+A+II + V I IH R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 81  SSPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQN---SNTTDPVFLFKLSLRVATDS 140
             PT S+ P E+TPAASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 141 FSKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRI 200
            + + +    L   T D  +K+A+ +C  + + AID +N+TVS+MEV    +K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 201 EDLKTWLSTAITDQETCLDAVRDLNQT--TVLQNLQTAMANSTEFTSNSLAIVTKILGLL 260
           +DL TWLS A+T   TCLDA+ +++ T   +   L++ M NSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 261 ADFNIPIH-RKLM 267
           +DF IPIH R+L+
Sbjct: 241 SDFGIPIHGRRLL 251

BLAST of HG10002413 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 4.3e-136
Identity = 275/582 (47.25%), Postives = 376/582 (64.60%), Query Frame = 0

Query: 30  YGKVDELEQQAFR--QKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSSSPTSSAP 89
           YG++DE EQ      +KT++R+ I+ +S ++L+ +++GAVVG        +++   S  P
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVG--------TTARDNSKKP 63

Query: 90  PTEITP---AASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSFSKLS 149
           PTE      + S+K LC VT +   C  +L    N++ + P  LFK +++V     SK+ 
Sbjct: 64  PTENNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVL 123

Query: 150 DYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKT 209
           D  S  N    D    AA+  C  +   A+D LN+T++S           S    +DL+T
Sbjct: 124 DGFS--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRT 183

Query: 210 WLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIPI 269
           WLS+  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG +AD     
Sbjct: 184 WLSSVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFR 243

Query: 270 HRKLMG-------LPEWVSSGDRRLLQENNV--TAHVTVSKDGKGDYTTIKDAVAAVPKK 329
            R+L+        + +      RRLL+  ++   A + V+KDG G Y TI +A+A V +K
Sbjct: 244 RRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEK 303

Query: 330 SKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVA 389
           +++  IIYVK+G+Y ENV ++K KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV 
Sbjct: 304 NEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVF 363

Query: 390 GKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDI 449
           GKGF+ +DMGFINTAGPAKHQAVA    +DLSV   C+ D +QDT+YAH+ RQFYRDC I
Sbjct: 364 GKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVI 423

Query: 450 TGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNES 509
            GT+DFIFGNAAVVFQ C+I PR+P+  Q NTITAQG+KD NQN+GISI  CT    +  
Sbjct: 424 LGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNL 483

Query: 510 LTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSV 569
               T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P+TIFY EY N+GPG+S 
Sbjct: 484 TDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGAST 543

Query: 570 DKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDT 595
             RVKW G ++SLT  EA KFTV+ F++G +WLPAT V F++
Sbjct: 544 KNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of HG10002413 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 261/577 (45.23%), Postives = 370/577 (64.12%), Query Frame = 0

Query: 30  YGKVDELEQQAF--RQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSSSPTSSAP 89
           YGKVDE E      R+KTR+ + I+ +S+V+L  ++IGAV G + HK++  +    ++  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 90  PTEITPAASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSFSKLSDYT 149
              +    S+K +C VT +   C  +L    N+++ +P  LF+ ++++   + +++S   
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 150 SNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKTWLS 209
           +  +S+ GD K    +  C  + +  ID LN+T++S      +     P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 210 TAITDQETCLDAVR-DLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIPIHR 269
           +A T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG +AD +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 270 KLMGLP--EWVSSGDRRLLQENNV--TAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII 329
           +L+     E      RRLLQ  ++   A + V+KDG G Y TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 330 YVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGK 389
           YVK+G+Y ENV ++K  WNV++ GDG +K+IVSGRLN IDGTPTF TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 390 DMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFI 449
           DMGFINTAGP+KHQAVA    +DL+    C+ + YQDTLY H+ RQFYR+C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 450 FGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYL 509
           FGN+A V Q+C+I PR+P+  Q NTITAQG+ D N N+GISI +C  S   +     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 510 GRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWV 569
           GRPWK FSTTVIM S +  F+   GW  W +G   P+TIFYGEY+NTGPG+S   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 570 GYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           G R  L+  EA +FTV+ F++G  WLPAT V F + L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of HG10002413 vs. ExPASy TrEMBL
Match: A0A1S3CBU9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.7e-308
Identity = 534/576 (92.71%), Postives = 558/576 (96.88%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NSNTGDPKVKAAI+IC+SVFEDAIDTLNDT+SSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ+LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAGKFTV TFLNGEDWLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of HG10002413 vs. ExPASy TrEMBL
Match: A0A5A7UGG5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 4.9e-308
Identity = 533/576 (92.53%), Postives = 557/576 (96.70%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S+VLLIAL+IGAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NSNTGDPKVKAAI+IC+SVFEDAIDTLNDT+SSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDR+LLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAGKFTV TFLNGEDWLPAT+V FDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of HG10002413 vs. ExPASy TrEMBL
Match: A0A0A0L956 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.5e-306
Identity = 529/576 (91.84%), Postives = 556/576 (96.53%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S T+S PPTE+TP ASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDS SKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDYTSN NS TGDPKV+AAI+ICRSVFEDAIDTLNDTVSSMEVD HSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQTTVLQ+LQTAMANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFII+
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEGIYEENVILDK+KWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIM+SEIG FL PVGWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRV+W G
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           Y+ SLTD+EAG+FTV TFLNGEDWLPAT+V FDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

BLAST of HG10002413 vs. ExPASy TrEMBL
Match: A0A6J1GVT9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.7e-300
Identity = 520/576 (90.28%), Postives = 546/576 (94.79%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MD+VKSFKGYGKVDELE QAFRQKTRRRLIILLLSIVLLIAL+IGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S TSS PPTE+ PAASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDY SNLNS+T D KVKAAI IC SVF+DAIDTLNDTVSSMEVD H EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLNQT VL+NLQ  MANSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLM  PEW+S GDRRLLQENNVT H TVSKDG GD+TTIK+AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEG+YEENVILDK+KWNVMMYGDG+T+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAAVVFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN+SLTAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FLQP+GWKEWVSGQDPP++IFYGEYQN+GPGS+VDKRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           YR SLTDAEA KFTVRTFLNGE+WLPATSVKFDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of HG10002413 vs. ExPASy TrEMBL
Match: A0A6J1IZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.1e-298
Identity = 519/576 (90.10%), Postives = 544/576 (94.44%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MD+VKSFKGYGKVDELE QAFRQKTRRRLIILLLSIVLLIAL+IGAVVGIVIHKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKL 140
           S TSS PPTE+ PAASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 141 SDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLK 200
           SDY SNLNS+T D KVKAAI IC SVF+DAIDTLNDTVSSMEVD H EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 201 TWLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 260
           TWLST ITDQETCLDA+RDLN T VL+NLQ  MANSTEFTSNSLAIVTKILGLLA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 261 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIY 320
           IHRKLM  PEWVS GDRRLLQENNVTAH TVSKDG  +YTTIK+AVAAVPKKSKERFIIY
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 321 VKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 380
           VKEG+YEENVILDK+KWNVMMYGDGRT+TIVSGRLNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 381 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 440
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 441 GNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYLG 500
           GNAA VFQNC IRPRQPL NQFNTITAQGKKDINQNSGISIQKCTFSAYN+SLTAPTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 501 RPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVG 560
           RPWKEFSTTVIMQSEIG FLQPVGWKEWVSGQDPP++IFYGEYQN+GPGS+V+KRVKW G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 561 YRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           YR SLTDAEA KFTVRTFLNGEDWLPATSVKFD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

BLAST of HG10002413 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 697.6 bits (1799), Expect = 8.8e-201
Identity = 359/590 (60.85%), Postives = 445/590 (75.42%), Query Frame = 0

Query: 21  MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSS 80
           MD+V SFKGYGKVDE +  A ++KTR+RL++L +S+V+LIA+II AVV  V+HK N + S
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHK-NKNES 60

Query: 81  SPTSSAPPTEITPAASLKTLCSVTQYPSSCQSS---LQNSNTTDPVFLFKLSLRVATDSF 140
           +P   +PP E+TP+ SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D  
Sbjct: 61  TP---SPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 141 SKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIE 200
             +SD    L+  T D ++K+A+ +C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 201 DLKTWLSTAITDQETCLDAVRDLNQ-------TTVLQNLQTAMANSTEFTSNSLAIVTKI 260
           DLKTWLS  +TD ETC D++ +L Q       +T+ QNL++AM+ STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 261 LGLLADFNIPIHRK----------LMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYT 320
           L  L+D  IPIHR+           +   +W     RRLLQ   +   VTV+ DG GD  
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 321 TIKDAVAAVPKKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDG 380
           T+ +AVA VPKKS + F+IYVK G Y ENV++DK+KWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 381 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 440
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 441 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGIS 500
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ CKI PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 501 IQKCTFSAYNESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFY 560
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM++ IGA ++P GW  WVSG DPP +I Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 561 GEYQNTGPGSSVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSV 591
           GEY+NTGPGS V +RVKW GY+  ++DAEA KFTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of HG10002413 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.4e-158
Identity = 292/527 (55.41%), Postives = 369/527 (70.02%), Query Frame = 0

Query: 72  IHKRNSSSSSPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQ----NSNTTDPVFLFK 131
           IH+R+   S     +P    TP++ L+T+C+VT YP+SC SS+     +  TTDP  LF+
Sbjct: 436 IHERHGVQSPRLRKSP--HPTPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFR 495

Query: 132 LSLRVATDSFSKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSM-EVDHH 191
           LSL+V  D  + +      L   T D  +K+A+ +C  VF+ A+D++NDT+SS+ EV   
Sbjct: 496 LSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISG 555

Query: 192 SEKFLSPSRIEDLKTWLSTAITDQETCLDAVRDLN-QTTVLQNLQTAMANSTEFTSNSLA 251
            +K L+ S I DL TWLS+A+TD  TC D + + N  + + Q L++AM NSTEFTSNSLA
Sbjct: 556 GKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLA 615

Query: 252 IVTKILGLLADFNIPIH-RKLM---GLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTT 311
           IV ++L   +   IP+  R+L+     P WV  G RRLLQ  N+T HVTV+ DG GD  T
Sbjct: 616 IVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVRT 675

Query: 312 IKDAVAAVPKKSKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGT 371
           + +AV  VPKK K  F+IYVK G Y ENV++ K+KWNV +YGDGR KTI+SG  N +DG 
Sbjct: 676 VNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGV 735

Query: 372 PTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAH 431
            TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFDGYQDTLY H
Sbjct: 736 RTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTH 795

Query: 432 SNRQFYRDCDITGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISI 491
           SNRQ+YR+CD+TGT+DFIFG   VVFQ C IRPRQPL NQFNTITA+G ++ NQN+GISI
Sbjct: 796 SNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISI 855

Query: 492 QKCTFSAYNESLTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQD-PPNTIFY 551
            +CT S  N ++TA TYLGRPWK FS TVIMQS IG+F+ P GW  W S  D PP TIFY
Sbjct: 856 HQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFY 915

Query: 552 GEYQNTGPGSSVDKRVKWVGYRSSLTDAEAGKFTVRTFLNGED-WLP 587
            EY+N+GPGS + KRVKW GY+   +D EA +FTV+ FL G+D W+P
Sbjct: 916 REYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959


HSP 2 Score: 207.2 bits (526), Expect = 3.6e-53
Identity = 125/253 (49.41%), Postives = 172/253 (67.98%), Query Frame = 0

Query: 21  MDTVKSF-KGYGKVDELEQQAFRQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSS 80
           MDTVKS  KGYGKVDE +  A ++KTR+RL  + +S+ +L+A+II + V I IH R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 81  SSPTSSAPPTEITPAASLKTLCSVTQYPSSCQSSLQN---SNTTDPVFLFKLSLRVATDS 140
             PT S+ P E+TPAASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 141 FSKLSDYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRI 200
            + + +    L   T D  +K+A+ +C  + + AID +N+TVS+MEV    +K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 201 EDLKTWLSTAITDQETCLDAVRDLNQT--TVLQNLQTAMANSTEFTSNSLAIVTKILGLL 260
           +DL TWLS A+T   TCLDA+ +++ T   +   L++ M NSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 261 ADFNIPIH-RKLM 267
           +DF IPIH R+L+
Sbjct: 241 SDFGIPIHGRRLL 251

BLAST of HG10002413 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 486.5 bits (1251), Expect = 3.1e-137
Identity = 275/582 (47.25%), Postives = 376/582 (64.60%), Query Frame = 0

Query: 30  YGKVDELEQQAFR--QKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSSSPTSSAP 89
           YG++DE EQ      +KT++R+ I+ +S ++L+ +++GAVVG        +++   S  P
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVG--------TTARDNSKKP 63

Query: 90  PTEITP---AASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSFSKLS 149
           PTE      + S+K LC VT +   C  +L    N++ + P  LFK +++V     SK+ 
Sbjct: 64  PTENNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVL 123

Query: 150 DYTSNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKT 209
           D  S  N    D    AA+  C  +   A+D LN+T++S           S    +DL+T
Sbjct: 124 DGFS--NGEHMDNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRT 183

Query: 210 WLSTAITDQETCLDAVRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIPI 269
           WLS+  T QETC+DA+ + N+ ++    +  + NSTE TSN+LAI+T  LG +AD     
Sbjct: 184 WLSSVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFR 243

Query: 270 HRKLMG-------LPEWVSSGDRRLLQENNV--TAHVTVSKDGKGDYTTIKDAVAAVPKK 329
            R+L+        + +      RRLL+  ++   A + V+KDG G Y TI +A+A V +K
Sbjct: 244 RRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEK 303

Query: 330 SKERFIIYVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVA 389
           +++  IIYVK+G+Y ENV ++K KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV 
Sbjct: 304 NEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVF 363

Query: 390 GKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDI 449
           GKGF+ +DMGFINTAGPAKHQAVA    +DLSV   C+ D +QDT+YAH+ RQFYRDC I
Sbjct: 364 GKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVI 423

Query: 450 TGTIDFIFGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNES 509
            GT+DFIFGNAAVVFQ C+I PR+P+  Q NTITAQG+KD NQN+GISI  CT    +  
Sbjct: 424 LGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNL 483

Query: 510 LTAPTYLGRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSV 569
               T+LGRPWK+FSTTVIM+S +  F+ P GW  W +G   P+TIFY EY N+GPG+S 
Sbjct: 484 TDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGAST 543

Query: 570 DKRVKWVGYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDT 595
             RVKW G ++SLT  EA KFTV+ F++G +WLPAT V F++
Sbjct: 544 KNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of HG10002413 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 464.9 bits (1195), Expect = 9.6e-131
Identity = 261/577 (45.23%), Postives = 370/577 (64.12%), Query Frame = 0

Query: 30  YGKVDELEQQAF--RQKTRRRLIILLLSIVLLIALIIGAVVGIVIHKRNSSSSSPTSSAP 89
           YGKVDE E      R+KTR+ + I+ +S+V+L  ++IGAV G + HK++  +    ++  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 90  PTEITPAASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSFSKLSDYT 149
              +    S+K +C VT +   C  +L    N+++ +P  LF+ ++++   + +++S   
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 150 SNLNSNTGDPKVKAAIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKTWLS 209
           +  +S+ GD K    +  C  + +  ID LN+T++S      +     P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 210 TAITDQETCLDAVR-DLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILGLLADFNIPIHR 269
           +A T Q TC++ +  D+         ++ + NSTE TSN+LAI+T  LG +AD +  + R
Sbjct: 185 SAGTYQRTCVETLAPDMRPFG-----ESHLKNSTELTSNALAIIT-WLGKIAD-SFKLRR 244

Query: 270 KLMGLP--EWVSSGDRRLLQENNV--TAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFII 329
           +L+     E      RRLLQ  ++   A + V+KDG G Y TIK A+  VP+KS++R II
Sbjct: 245 RLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTII 304

Query: 330 YVKEGIYEENVILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGK 389
           YVK+G+Y ENV ++K  WNV++ GDG +K+IVSGRLN IDGTPTF TATFAV GKGF+ +
Sbjct: 305 YVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMAR 364

Query: 390 DMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFI 449
           DMGFINTAGP+KHQAVA    +DL+    C+ + YQDTLY H+ RQFYR+C I GT+DFI
Sbjct: 365 DMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFI 424

Query: 450 FGNAAVVFQNCKIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESLTAPTYL 509
           FGN+A V Q+C+I PR+P+  Q NTITAQG+ D N N+GISI +C  S   +     T+L
Sbjct: 425 FGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFL 484

Query: 510 GRPWKEFSTTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWV 569
           GRPWK FSTTVIM S +  F+   GW  W +G   P+TIFYGEY+NTGPG+S   RVKW 
Sbjct: 485 GRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRVKWK 544

Query: 570 GYRSSLTDAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
           G R  L+  EA +FTV+ F++G  WLPAT V F + L
Sbjct: 545 GLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of HG10002413 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 392.9 bits (1008), Expect = 4.6e-109
Identity = 214/510 (41.96%), Postives = 300/510 (58.82%), Query Frame = 0

Query: 99  TLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSFSKLSDYTSNLNSNTGDPKVKA 158
           +LCS  +   S    +Q      P   F  + +   D+ +K     S  +   G  +V  
Sbjct: 20  SLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSN 79

Query: 159 AIEICRSVFEDAIDTLNDTVSSMEVDHHSEKFLSPSRIEDLKTWLSTAITDQETCLDAVR 218
           AI  C  + + A + L+  +S+ +  +  +         DL+TW+S A+++Q+TCLD   
Sbjct: 80  AIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVG-SDLRTWISAALSNQDTCLDGFE 139

Query: 219 DLNQTTVLQNLQTAMANSTEFTSNSLAIVT-------KILGLLADFNIPIHRKLMGLPEW 278
             N   +++ +     +    T  +L  +        K   + A      H      P W
Sbjct: 140 GTN--GIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSW 199

Query: 279 VSSGDRRLLQENNVT-AHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIYVKEGIYEENV 338
           V  GDR+LLQ +N+T A   V+ DG G++TTI DAV A P  S +R++I+VK G+Y ENV
Sbjct: 200 VKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENV 259

Query: 339 ILDKNKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPA 398
            + K KWN+MM GDG   T+++G  +FIDG  TF +ATFAV+G+GFI +D+ F NTAGP 
Sbjct: 260 EIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPE 319

Query: 399 KHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNC 458
           KHQAVA RS +DL V   C+  GYQDTLYAHS RQF+R+C ITGT+DFIFG+A  VFQ+C
Sbjct: 320 KHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSC 379

Query: 459 KIRPRQPLLNQFNTITAQGKKDINQNSGISIQKCTFSAYNESL----TAPTYLGRPWKEF 518
           +I+ +Q L NQ N+ITAQG+KD N+ +G +IQ    +A  + L    T  TYLGRPWK +
Sbjct: 380 QIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLY 439

Query: 519 STTVIMQSEIGAFLQPVGWKEWVSGQDPPNTIFYGEYQNTGPGSSVDKRVKWVGYRSSLT 578
           S TV MQ+ +   + PVGW EW +G    +T++YGEY N+GPG+S+D+RVKW GY    T
Sbjct: 440 SRTVFMQNYMSDAINPVGWLEW-NGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNT 499

Query: 579 DAEAGKFTVRTFLNGEDWLPATSVKFDTSL 597
            AEA  FTV   + G  WLP+T + F   L
Sbjct: 500 SAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877946.19.2e-30993.40pectinesterase 3 [Benincasa hispida][more]
XP_008460354.13.5e-30892.71PREDICTED: pectinesterase 3 [Cucumis melo][more]
KAA0053577.11.0e-30792.53pectinesterase 3 [Cucumis melo var. makuwa] >TYK19167.1 pectinesterase 3 [Cucumi... [more]
XP_004144445.17.2e-30691.84pectinesterase 3 [Cucumis sativus] >KGN58475.1 hypothetical protein Csa_001265 [... [more]
XP_023528356.11.2e-30090.45pectinesterase 3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q431111.2e-20762.97Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1[more]
Q438671.2e-19960.85Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1[more]
Q9LUL87.6e-15755.41Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3... [more]
Q9FF784.3e-13647.25Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... [more]
Q9SG771.3e-12945.23Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3CBU91.7e-30892.71Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1[more]
A0A5A7UGG54.9e-30892.53Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970... [more]
A0A0A0L9563.5e-30691.84Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1[more]
A0A6J1GVT93.7e-30090.28Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1[more]
A0A6J1IZN02.1e-29890.10Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G53840.18.8e-20160.85pectin methylesterase 1 [more]
AT3G14300.15.4e-15855.41pectinesterase family protein [more]
AT5G04960.13.1e-13747.25Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10710.19.6e-13145.23root hair specific 12 [more]
AT3G43270.14.6e-10941.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 92..246
e-value: 5.8E-35
score: 132.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 97..246
e-value: 1.1E-25
score: 90.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 77..250
e-value: 3.9E-22
score: 76.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 288..581
e-value: 4.9E-138
score: 458.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 95..251
e-value: 7.4E-33
score: 115.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 97..251
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 282..596
e-value: 3.3E-114
score: 383.5
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 28..593
NoneNo IPR availablePANTHERPTHR31707:SF219PECTINESTERASE 1-RELATEDcoord: 28..593
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 99..250
e-value: 3.31984E-42
score: 147.2
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 432..441
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 286..592

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002413.1HG10002413.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity