HG10002412 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002412
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectinesterase
LocationChr11: 6479740 .. 6484700 (+)
RNA-Seq ExpressionHG10002412
SyntenyHG10002412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACAAACCGAGGACTCCACCGCCGCCGCCGCCGGTAGCCCCATTTCCATTTCCAACATAAAATTAGCGAAGCTCTTGATCTTAGCCATGTCGGCTGCACTTCTCCTGGCGGGCGTCTCTTCCGACGAGCACGTGAGAAGCACGTGCGCCGTGACGCTGTACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGCAAGTAGGAGTTCAAAAGAGGCAATCGAAGCATCGGTGAATATTACGATTGAAGCAGTGAAGGATAACTATAAAAGCATCAAGAAATTGGTTAAGACATTGAAGAATCTGACAAAGCGAGAGAAAATTGCTCTCAACGACTGTCTAGAAACTGGGGAGGAGACGCTGAGAGAGCTTTATGAGGTTGTTGAAGATCTCCATGAATACCCTAATAAGAAATCTCTCTCACAGTACGCCGATGACCTCAAAACCTTCCTTAGTTCCGCCATCACTAATCAGGTACCCTCCATTAATTAGTTCCAATTTTGAAAAAAAAAAAACCAACTACCTCAAAATTCCAATTTTGAAGTTTCCTCTTTAATTTTACTCTTAAATATTCAATTCAATATTATTGAAAAATATTTCACTAAAACTATTTTTTTTATAGAAATTTTCCATAGATATAAAAAATGTCAAATTATTTACCGAAAATAGCAAAAAAAAAAAAAAAAATATACGGATGGACATTGATAGACTTCTATTAACATCTATCCGTGATAGACTTCTATCATTTCTATCATTAATAGACCATGATAGACTTCTATCATAATCTATCATAACTATCTAAACATTTTTCTATTTTGTGTAAATAATTTTCTTATATTTCTATTTTTAAAAATTCTCCAAATATTTATTATTATTATTTGTTTTCAAAATTTAAAATGTATTTATGTTTAAAGGTCTACGAGGTTTCAATTTCGTATCTCATAAGTCTATATCATATAATTTCAAATTTTAAAAATTAATTGATATATTATTTTGAATTTTGAATTTTGTGTGTCACACATTTTAAACTTTTATTTTTTGTGTCTAGTAGATTTCTAGATCTTACAAGATATTAGAGAGTTTGATAATCTATTAGACATAAAATTTAACGTATAGGTATATGTTAGACAAATTTTAGCATTTAAGTACTTATTTGGATACAAAATTAAAAATTGAGATACTTATTAAATACATTTTAAACTTTAGTATTCAAATAGACACGAAAATAAATTTAGAACATACAAAATATTGATTTTGCCACAAATCAATATAGATGCTATTTGTCATAAATCAAGTAGACATGAATATTAAATCTCACTTATTATCATTACAGAAGATCTCCCACTATGATGATGGTTGAACAAAAAAAAAAAAAAAAAAGGTCATTTTTGGTATTTATCTCTATTAGTTTACTTGTGAGGCTCACAAGTAAAATTGGAAGGGTCAATAAAGTATGAAATTGATTTTTTTTTTAATCATAAAGACTACAAAAGTTTTGGGTAAACAAAAAGTGAAGTTAATGTACTATATATAGACCTATAAAATAAAATAGATAAAGTAAGTAGCATTAGATTTAGCAATGACATGTTTTTAATTACAATAATTCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGCTGATAAGAAGGTACGAGAGTCATTAGAAGCCGGCCTAATTCACGTCGAAAAGCTTTGTAGCATTGCTTTAGCCTTGATTAAGAATCTAACCGACACCGACATTGCCAATCACAACAACCTCAATAGAAAGCAGTTGACGGAGATCAACAACGGTCGTAGTGACGACAATGGAATTAACTGGCCGAATTGGATGTCGCATGGTGACCGAAGGCTTTTGCAAGCGTCGTCCGCAGCCACTCCCAACGCGGTAGTTGCAGCCGACGGGAGTGGTGATTTCCGGACGGTGTTGGAGGCAGTTGTTGCCGCACCTAATAGAAGTAGCCAGAGGTATATAATAAGAATAAAGGCAGGAGTTTATAGAGAGAATGTGAATGTGCCGAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACCACTATAATCACTGGGAACAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCTGCCACCGTTGGTATGTCATCTTATACTGTTCTTTTCTAACCCATAATTCTCAAAATATGAATTTTATCAACTTTGGCTTCTTAATTAAATTAAATGCAATTCATTTAACTCAATTAAACTATACACCACCACATTAGAAATTTAGAGGGAAAGAAATAGAAATTAAAAACTATTATAACCTATAGACTATTTTCATATCGTCAAAATGACTCGAAATGATATAAATGATTTTAACTATTTCAAAATTAGTACCAAACATGTACTTAAAACCATTAGTTTCAGCTAATCTTGAATTGAGAATGAATACATTATAAATATATTGAGGATTAAAACAATATTTTTTTAGTACAATGAAGAGTACATATAGAGATTTGAACCATAGACTTCTTGATCACTAACACATTTATATGTCAGTTGAGTTATGTTTGTTTTGACGATTAAAATAATACTTAGGACAAAAAAAAAATTGATGAATCCTAAGATATCATAATATACATATTTTACTCTACTAGTAAAAAACTTGTGGACCATTTTTGGAGCCTTCCTACTTCTACTTGTACTCCATCGTAGTCGTACCAGCAATATGAATTGCCATTTAATTGTAATGGTCACTCTTAAATTTTATTCACAATAACAGTACAAAACTTAGCTGCATATATATTTTTCTTTCAATAAAAAAATCACATAAATCTTATCTTATCTTAAATATAAAATAATAATACATGGGAAAGAAACTAAACTTTTACTCTAAACAGGACACTTTGTCCCAATTTTCATCTTATATTAGTTAAATTGTGATTTTTTACTCCATTCAATTTTCGTGATTCATCCTTTAATTCTAACCCAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTCCATAAATTTATTAATTTTCAATATTATTAAGTTTTTACAATAACCATTAAACAACTTTGCTCAAATTTACAAATTTTAACAAGAATTCAACTCTATTGACATTTTTCTTTTAAATCTGGTCCATTTGCATATGTTTTATCATAAATTGAAAGTATTTGTGAACACTTGTTCTAACAATTTTTTAAAAAAAAAATGTCATAAGATAACTATTCTGAGATTTTTTTTTTTTTTAATTTAAGGTGAAAATTACAACACTTGTAAATTAAAAGTTTACATTAAAAATTCTTACTACTTAACAATATAATAGATGATAGATATTAACGCGTTTGAAAATTATTTCTGCACCTAATGTCCAATCTAGAAATAAATGTATAGCTTTAACTTCTGCATTTGACATTTATGCACACGAAAAAAAAAAAAAAGAATTACAGAAATATTTGAAAGTGAAACCCACGTGCCGATGATCCGACACACTTTCATTATCACCTCTCATTTATATATATATATATATATATATACATAAAAAATAAAAAAATTATTTATTCAGATAGTAAAATTTTTAGATAGTTGTGATATATGCAGATAGAAGTCTATCAGAATCTATCAGCGATAAAAATGATAGAAGTCTATAACTGATAGACGCTGATAGAAGTCTATCAATGTCTATTAGTATTTTTTTTTGTCATTTTCGGTAAATAGTTTGACATTTTTCTATTTGTCAAAATTTCTCTCTCTATATATATATATATACACACTTCACGATATGTAAATGAGGGATTTAATCTTAGTTTGAGGTGGATAGTACAAGTTTACTTAATATACTCTTGTTGGTTTTTTAAAAGTATTTTATTATTTTGCGGTCTACTTATCCAATCACAATTTACCACATGGTAGATCTTAATTCTTTTTTGTTGAAGTGAGTTAGAAAAAAATAGGACTCTCAAGATACACTCATAATATTGTACACATAATCATTGCATAAGTGTTTTTCAGAAATACTTAGGTGCATTTTGAGTTGCATTTATCTTTGTGCATCTCATAAGTTGTCCAATCATAATTTGCTATATAAAAAAAATTCTTTTTTACTTATTTTTATTCATTGTTGGTGTGTTATGAGAGTGAGATGCATAAAAATGAGTATATCTTAAGATGCACTATTGTGGAATTAAAAAATAAAGGAGTACGCTCAAAAGTTTCTCTGATAATGTGGGATGTGAAATTGCAGCGGCGGTTGGAGAAAGGTTCTTGGCGAGAGACCTTACGTTTCAAAACACCGCCGGTCCATCGAAGCACCAAGCGGTAGCTCTCCGTGTCGGCTCTGATCTCTCGGCTTTTTACCGCTGCGATATGCTCGCCTATCAAGACACACTCTATGTCCACTCCAACCGCCAATTCTACGTCAAATGCATCATCATTGGCTCCGTCGACTTCATTTTCGGCAACGCTGCTGCTGTCATCCAAGATTGTGATATCCACGCCCGGCGCCCCAATCCTGGCCAGAGGAACATGGTCACTGCACAGGGAAGAACAGACCCAAATCAGAATACTGGGATTGTCATCCAGAAATGTCGAATCGGAACCACCTCCGATTTGCGACCGGTGATTAGTAATTTCCCCACGTTTCTAGGGCGGCCATGGCAGAGATACTCAAGGACAGTGGTTATGCATACGAGCATTAGCAATGTGATTGATCCAGCAGGGTGGCATGTTTGGGATGGAAATTTTGCGCTTGATACTTTATTTTACGCTGAGTATCAAAATAATGGGCCTGGTGCTGATACGTCTAGGAGAGTGACGTGGAAGGGGTATAAGGTTATTACCAATGCGGCGGAAGCTGAGGCCTTTACGGCTGGAAATTTCATCAGCGGCGGCACTTGGCTGAGCTCCACTGGATTCCCATTTTCACTTGGGCTATAA

mRNA sequence

ATGGCACAAACCGAGGACTCCACCGCCGCCGCCGCCGGTAGCCCCATTTCCATTTCCAACATAAAATTAGCGAAGCTCTTGATCTTAGCCATGTCGGCTGCACTTCTCCTGGCGGGCGTCTCTTCCGACGAGCACGTGAGAAGCACGTGCGCCGTGACGCTGTACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGCAAGTAGGAGTTCAAAAGAGGCAATCGAAGCATCGGTGAATATTACGATTGAAGCAGTGAAGGATAACTATAAAAGCATCAAGAAATTGGTTAAGACATTGAAGAATCTGACAAAGCGAGAGAAAATTGCTCTCAACGACTGTCTAGAAACTGGGGAGGAGACGCTGAGAGAGCTTTATGAGGTTGTTGAAGATCTCCATGAATACCCTAATAAGAAATCTCTCTCACAGTACGCCGATGACCTCAAAACCTTCCTTAGTTCCGCCATCACTAATCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGCTGATAAGAAGGTACGAGAGTCATTAGAAGCCGGCCTAATTCACGTCGAAAAGCTTTGTAGCATTGCTTTAGCCTTGATTAAGAATCTAACCGACACCGACATTGCCAATCACAACAACCTCAATAGAAAGCAGTTGACGGAGATCAACAACGGTCGTAGTGACGACAATGGAATTAACTGGCCGAATTGGATGTCGCATGGTGACCGAAGGCTTTTGCAAGCGTCGTCCGCAGCCACTCCCAACGCGGTAGTTGCAGCCGACGGGAGTGGTGATTTCCGGACGGTGTTGGAGGCAGTTGTTGCCGCACCTAATAGAAGTAGCCAGAGGTATATAATAAGAATAAAGGCAGGAGTTTATAGAGAGAATGTGAATGTGCCGAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACCACTATAATCACTGGGAACAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCTGCCACCGTTGCGGCGGTTGGAGAAAGGTTCTTGGCGAGAGACCTTACGTTTCAAAACACCGCCGGTCCATCGAAGCACCAAGCGGTAGCTCTCCGTGTCGGCTCTGATCTCTCGGCTTTTTACCGCTGCGATATGCTCGCCTATCAAGACACACTCTATGTCCACTCCAACCGCCAATTCTACGTCAAATGCATCATCATTGGCTCCGTCGACTTCATTTTCGGCAACGCTGCTGCTGTCATCCAAGATTGTGATATCCACGCCCGGCGCCCCAATCCTGGCCAGAGGAACATGGTCACTGCACAGGGAAGAACAGACCCAAATCAGAATACTGGGATTGTCATCCAGAAATGTCGAATCGGAACCACCTCCGATTTGCGACCGGTGATTAGTAATTTCCCCACGTTTCTAGGGCGGCCATGGCAGAGATACTCAAGGACAGTGGTTATGCATACGAGCATTAGCAATGTGATTGATCCAGCAGGGTGGCATGTTTGGGATGGAAATTTTGCGCTTGATACTTTATTTTACGCTGAGTATCAAAATAATGGGCCTGGTGCTGATACGTCTAGGAGAGTGACGTGGAAGGGGTATAAGGTTATTACCAATGCGGCGGAAGCTGAGGCCTTTACGGCTGGAAATTTCATCAGCGGCGGCACTTGGCTGAGCTCCACTGGATTCCCATTTTCACTTGGGCTATAA

Coding sequence (CDS)

ATGGCACAAACCGAGGACTCCACCGCCGCCGCCGCCGGTAGCCCCATTTCCATTTCCAACATAAAATTAGCGAAGCTCTTGATCTTAGCCATGTCGGCTGCACTTCTCCTGGCGGGCGTCTCTTCCGACGAGCACGTGAGAAGCACGTGCGCCGTGACGCTGTACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGCAAGTAGGAGTTCAAAAGAGGCAATCGAAGCATCGGTGAATATTACGATTGAAGCAGTGAAGGATAACTATAAAAGCATCAAGAAATTGGTTAAGACATTGAAGAATCTGACAAAGCGAGAGAAAATTGCTCTCAACGACTGTCTAGAAACTGGGGAGGAGACGCTGAGAGAGCTTTATGAGGTTGTTGAAGATCTCCATGAATACCCTAATAAGAAATCTCTCTCACAGTACGCCGATGACCTCAAAACCTTCCTTAGTTCCGCCATCACTAATCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGCTGATAAGAAGGTACGAGAGTCATTAGAAGCCGGCCTAATTCACGTCGAAAAGCTTTGTAGCATTGCTTTAGCCTTGATTAAGAATCTAACCGACACCGACATTGCCAATCACAACAACCTCAATAGAAAGCAGTTGACGGAGATCAACAACGGTCGTAGTGACGACAATGGAATTAACTGGCCGAATTGGATGTCGCATGGTGACCGAAGGCTTTTGCAAGCGTCGTCCGCAGCCACTCCCAACGCGGTAGTTGCAGCCGACGGGAGTGGTGATTTCCGGACGGTGTTGGAGGCAGTTGTTGCCGCACCTAATAGAAGTAGCCAGAGGTATATAATAAGAATAAAGGCAGGAGTTTATAGAGAGAATGTGAATGTGCCGAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACCACTATAATCACTGGGAACAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCTGCCACCGTTGCGGCGGTTGGAGAAAGGTTCTTGGCGAGAGACCTTACGTTTCAAAACACCGCCGGTCCATCGAAGCACCAAGCGGTAGCTCTCCGTGTCGGCTCTGATCTCTCGGCTTTTTACCGCTGCGATATGCTCGCCTATCAAGACACACTCTATGTCCACTCCAACCGCCAATTCTACGTCAAATGCATCATCATTGGCTCCGTCGACTTCATTTTCGGCAACGCTGCTGCTGTCATCCAAGATTGTGATATCCACGCCCGGCGCCCCAATCCTGGCCAGAGGAACATGGTCACTGCACAGGGAAGAACAGACCCAAATCAGAATACTGGGATTGTCATCCAGAAATGTCGAATCGGAACCACCTCCGATTTGCGACCGGTGATTAGTAATTTCCCCACGTTTCTAGGGCGGCCATGGCAGAGATACTCAAGGACAGTGGTTATGCATACGAGCATTAGCAATGTGATTGATCCAGCAGGGTGGCATGTTTGGGATGGAAATTTTGCGCTTGATACTTTATTTTACGCTGAGTATCAAAATAATGGGCCTGGTGCTGATACGTCTAGGAGAGTGACGTGGAAGGGGTATAAGGTTATTACCAATGCGGCGGAAGCTGAGGCCTTTACGGCTGGAAATTTCATCAGCGGCGGCACTTGGCTGAGCTCCACTGGATTCCCATTTTCACTTGGGCTATAA

Protein sequence

MAQTEDSTAAAAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCETTISTAVASRSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL
Homology
BLAST of HG10002412 vs. NCBI nr
Match: XP_038878011.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 963.8 bits (2490), Expect = 6.7e-277
Identity = 499/572 (87.24%), Postives = 534/572 (93.36%), Query Frame = 0

Query: 1   MAQTEDSTAAA---AGSPISISNIKLAKLLILAMSAALLLAGV-SSDEHVRSTCAVTLYP 60
           MAQT+DS AA    +G PISISNIK +KLL+LAMSA LL+AGV SSDE VRSTCAVTLYP
Sbjct: 1   MAQTDDSAAAGVSFSGRPISISNIK-SKLLVLAMSATLLVAGVSSSDEQVRSTCAVTLYP 60

Query: 61  ELCETTISTAVASR-SSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDC 120
           ELCE TIST   SR SSK+AIEASVNITI AVKDNYK ++KLVKT+KNLTKREKIALNDC
Sbjct: 61  ELCEKTISTGGGSRWSSKDAIEASVNITIGAVKDNYKRMEKLVKTVKNLTKREKIALNDC 120

Query: 121 LETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKK 180
           LE+ EETLRELYEVVEDLHEYPNKKSL+QYADDLKT LSSAITNQETCVDGFSHDKADKK
Sbjct: 121 LESEEETLRELYEVVEDLHEYPNKKSLTQYADDLKTLLSSAITNQETCVDGFSHDKADKK 180

Query: 181 VRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNN--LNRKQLTEINN-GRSDDNGINW 240
           VRES+EAGLIH++KLCSIALALIKNLTDTDIANHN   LNRK L E+NN GR +D+ INW
Sbjct: 181 VRESIEAGLIHIKKLCSIALALIKNLTDTDIANHNYNVLNRKHLAEVNNGGRGNDDRINW 240

Query: 241 PNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYR 300
           P+WMS GDRRLLQASS ATP+ VVAADG+G+FRTV EAV AAP+RSS+RYIIRIKAGVYR
Sbjct: 241 PDWMSSGDRRLLQASSTATPDVVVAADGTGNFRTVSEAVAAAPSRSSRRYIIRIKAGVYR 300

Query: 301 ENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTA 360
           ENVNVPSSK N+MFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQNTA
Sbjct: 301 ENVNVPSSKSNLMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGARFLARDITFQNTA 360

Query: 361 GPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVI 420
           GPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFY+KCII+G++DFIFGNAAAVI
Sbjct: 361 GPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYIKCIIVGTIDFIFGNAAAVI 420

Query: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPW 480
           QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+ VI NFPTFLGRPW
Sbjct: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQQVIGNFPTFLGRPW 480

Query: 481 QRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVI 540
           QRYSRTVVM TSISNVIDPAGWHVW+GNFALDTLFYAEYQN+G GADTSRRVTWKGY+VI
Sbjct: 481 QRYSRTVVMQTSISNVIDPAGWHVWNGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVI 540

Query: 541 TNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           T+AAEA+AFTAGNFISGGTWLSSTGFPFSLGL
Sbjct: 541 TSAAEAQAFTAGNFISGGTWLSSTGFPFSLGL 571

BLAST of HG10002412 vs. NCBI nr
Match: XP_008460358.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0053588.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK19178.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 924.1 bits (2387), Expect = 5.9e-265
Identity = 477/558 (85.48%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 11  AAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCETTISTAVASR 70
           AAG P S ++     LL+ A+S  LLLA VSSD+HVRS CA+TLYPELCETTISTAV S 
Sbjct: 9   AAGVPFSCNS--KTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTISTAVGS- 68

Query: 71  SSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGEETLRELYEVV 130
           SSKEAIEASVNITI AV DNY+ +KKL+KT  +LTKREKIAL+DCLETGEETLRELYEVV
Sbjct: 69  SSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRELYEVV 128

Query: 131 EDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKL 190
           ED++EYPNKKSLS+YADDLKT LSSAITNQETC+DGFSHDKADKKVRESL+ GLIH+EKL
Sbjct: 129 EDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLIHIEKL 188

Query: 191 CSIALALIKNLTDTDIANHNN----LNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQA 250
           CSIALALIKNLTDTDIAN NN    L+RKQL E N     D+ INWP+WMS  DRRLLQA
Sbjct: 189 CSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNT----DDRINWPDWMSPNDRRLLQA 248

Query: 251 SSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMF 310
           SS ATP+ VVAADGSGDFRT+ EAV AAP+RSSQRYIIRIKAGVYRENVNV SSKRNIMF
Sbjct: 249 SSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRNIMF 308

Query: 311 WGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGS 370
           WGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGS
Sbjct: 309 WGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGS 368

Query: 371 DLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQ 430
           DLSAFYRCDMLAYQDTLYVHSNRQFYV+CII+G++DFIFGNAAAVIQDCDIHARRPNPGQ
Sbjct: 369 DLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQ 428

Query: 431 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSIS 490
           RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVM TSIS
Sbjct: 429 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSIS 488

Query: 491 NVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNF 550
           NVIDPAGWHVWDGNFALDTLFYAEYQN+G GADTSRRV WKG++V+T AAEAEAFTAGNF
Sbjct: 489 NVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTAGNF 548

Query: 551 ISGGTWLSSTGFPFSLGL 565
           I GGTWLSSTGFPFSLGL
Sbjct: 549 IGGGTWLSSTGFPFSLGL 559

BLAST of HG10002412 vs. NCBI nr
Match: XP_004144447.2 (pectinesterase [Cucumis sativus] >KGN58477.1 hypothetical protein Csa_002294 [Cucumis sativus])

HSP 1 Score: 918.7 bits (2373), Expect = 2.5e-263
Identity = 473/570 (82.98%), Postives = 510/570 (89.47%), Query Frame = 0

Query: 1   MAQTEDSTAAAAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCE 60
           M++ +D+ AA     +  S      LL+ A+S  LLLA VSSD+HV S CA+TLYPELCE
Sbjct: 1   MSKPDDTPAAG----VPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCE 60

Query: 61  TTISTAVASRSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGE 120
           TTISTAV S SSKEAIEASVNITI AVKDNYK ++KL+KT+KNLTKR+KIA +DCLETGE
Sbjct: 61  TTISTAVGS-SSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGE 120

Query: 121 ETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESL 180
           ETLRELYEVVED++EYP KKSLS+YADDLKT LSS ITNQETCVDGFSHDK DKKVRESL
Sbjct: 121 ETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESL 180

Query: 181 EAGLIHVEKLCSIALALIKNLTDTDIANHNN------LNRKQLTEINNGRSDDNGINWPN 240
           + GLIH+EKLCSIALALIKNLTDTDIAN NN      LNRKQL E    +  ++GI WP+
Sbjct: 181 KEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEE----KKMEDGIKWPD 240

Query: 241 WMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYREN 300
           WMS  DRRLLQASS ATP+ VVAADGSGDFRT+ EAV AAP+RSS+RYIIRIKAGVYREN
Sbjct: 241 WMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYREN 300

Query: 301 VNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGP 360
           VNV SSKRNIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTAGP
Sbjct: 301 VNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGP 360

Query: 361 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQD 420
           SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV+CII+G++DFIFGNAAAVIQD
Sbjct: 361 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQD 420

Query: 421 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 480
           CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR
Sbjct: 421 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 480

Query: 481 YSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITN 540
           YSRTVVM TSISNVIDPAGWHVWDGNFALDTLFYAEYQN+G GADTSRRV WKG++V+T 
Sbjct: 481 YSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTR 540

Query: 541 AAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           AAEAEAFTAGNFI GGTWLSSTGFPFSLGL
Sbjct: 541 AAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of HG10002412 vs. NCBI nr
Match: XP_023526589.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.4 bits (2367), Expect = 1.2e-262
Identity = 477/575 (82.96%), Postives = 515/575 (89.57%), Query Frame = 0

Query: 1   MAQTEDSTAAAA---GSPISISNIKLAKLLILAMSAALLLAGVSSDEH---VRSTCAVTL 60
           MAQT+DST A A   GSP SISN K   L ILA+SAA+LLAGVSSD+H   VRSTCAVTL
Sbjct: 1   MAQTDDSTVAGAPSTGSPTSISN-KKHNLFILALSAAVLLAGVSSDDHVTAVRSTCAVTL 60

Query: 61  YPELCETTISTAVAS-----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREK 120
           YPELC++TISTAV +      SSK+ IEASVNITI AVK NYKSIKKL++T K+LTKREK
Sbjct: 61  YPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREK 120

Query: 121 IALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSH 180
           IAL+DCLETGEETLRELYEVVEDL+EYP KKSL QYADDLKT LSSAITNQETCVDGFSH
Sbjct: 121 IALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 180

Query: 181 DKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNG 240
           D+ADKK+RESL AG IHVEKLCS+ALALIKNLTDTDIANH N+  + +          + 
Sbjct: 181 DRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAM--------SE 240

Query: 241 INWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAG 300
            NWP+WMS GDRRLLQASS ATP+ VVAADGSGDF+TV  AV AAP+RSS+RYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRRLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQ 360
           VYRENVNVPSSK NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAA 420
           NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CII+G+VDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGY 540
           RPWQRYSRTVVM TSISNVIDPAGWH+WDGNFALDTLFYAEYQN+G GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGY 540

Query: 541 KVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           +VI++AA+AE+FTAG FISGGTWLSSTGFPFSLGL
Sbjct: 541 RVISSAADAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of HG10002412 vs. NCBI nr
Match: XP_022980581.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 914.1 bits (2361), Expect = 6.1e-262
Identity = 478/575 (83.13%), Postives = 512/575 (89.04%), Query Frame = 0

Query: 1   MAQTEDSTAAAA---GSPISISNIKLAKLLILAMSAALLLAGVSSDEH---VRSTCAVTL 60
           MAQT+DST A A    SP SIS  K   L ILA+SAALLL GVSSD+H   VRSTCAVTL
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISK-KKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTL 60

Query: 61  YPELCETTISTAVAS-----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREK 120
           YPELC++TISTAV +      SSK+ IEASVNITI AVK NYKSIKKLV+T KNLTKREK
Sbjct: 61  YPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREK 120

Query: 121 IALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSH 180
           IAL+DCLETGEETLRELYEVVEDL+EYP KKSL QYADDLKT LSSAITNQETCVDGFSH
Sbjct: 121 IALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 180

Query: 181 DKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNG 240
           D+ADKK+RESL AG IHVEKLCS+ALALIKNLTDTDIANH  L    +   +   S+   
Sbjct: 181 DRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE--- 240

Query: 241 INWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAG 300
            NWP+WMS GDR+LLQASS ATP+ VVAADGSGDF+TV  AV AAP+RSS+RYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQ 360
           VYRENVNVPSSK NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAA 420
           NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CII+G+VDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGY 540
           RPWQRYSRTVVM TSISNVIDPAGWH+WDGNFALDTLFYAE+QN+G GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGY 540

Query: 541 KVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           +VI++AAEAE FTAG FISGGTWLSSTGFPFSLGL
Sbjct: 541 RVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

BLAST of HG10002412 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.9e-202
Identity = 363/538 (67.47%), Postives = 430/538 (79.93%), Query Frame = 0

Query: 37  LAGVSSDEH--VRSTCAVTLYPELCETTI----STAVASRSSKEAIEASVNITIEAVKDN 96
           +  +SS  H  V+S C+ TL+PELC + I      +    S K+ IE S+NIT++AV+ N
Sbjct: 55  IMAISSSAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRN 114

Query: 97  YKSIKKLVKTLKNLTKREKIALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLK 156
           Y ++K+L+KT K LT REK+AL+DCLET +ETL EL+  VEDL  YPNKKSL ++ +DLK
Sbjct: 115 YYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLK 174

Query: 157 TFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHN 216
           T +SSAITNQETC+DGFSHD+ADKKVR+ L  G  HVEK+CS ALA+I N+TDTDIAN  
Sbjct: 175 TLISSAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEM 234

Query: 217 NL----NRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRT 276
            L    N ++L E       DNG  WP W+S GDRRLLQ SS  TP+ VVAADGSGD++T
Sbjct: 235 KLSAPANNRKLVE-------DNG-EWPEWLSAGDRRLLQ-SSTVTPDVVVAADGSGDYKT 294

Query: 277 VLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGS 336
           V EAV  AP +SS+RY+IRIKAGVYRENV+VP  K NIMF GDG++ TIIT +RNV DGS
Sbjct: 295 VSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGS 354

Query: 337 TTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 396
           TTF+SATV  V  + LARD+TFQNTAG SKHQAVAL VGSDLSAFYRCDMLAYQDTLYVH
Sbjct: 355 TTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVH 414

Query: 397 SNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 456
           SNRQF+V+C++ G+VDFIFGN AAV QDCDIHARRP  GQ+NMVTAQGRTDPNQNTGIVI
Sbjct: 415 SNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVI 474

Query: 457 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTL 516
           QKCRIG TSDLRPV  +FPT+LGRPW+ YSRTV+M +SI++VI PAGWH W+GNFALDTL
Sbjct: 475 QKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTL 534

Query: 517 FYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           FY EY N G GA TS RV WKG+KVIT++ EA+A+T G FI+GG+WLSSTGFPFSLGL
Sbjct: 535 FYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of HG10002412 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.3e-202
Identity = 373/573 (65.10%), Postives = 448/573 (78.18%), Query Frame = 0

Query: 17  SISNI-KLAKLLILAMSAALL--------LAGVSSDEH------------VRSTCAVTLY 76
           +ISNI K  K L LA+ A LL        +AGV+S ++            ++S+C+ T Y
Sbjct: 19  NISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRY 78

Query: 77  PELCETTISTAVAS----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIA 136
           P+LC + I+    +     S K+ IE S+NIT  AV+ NY  I+KL+K   NLTKREK+A
Sbjct: 79  PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKR-TNLTKREKVA 138

Query: 137 LNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDK 196
           L+DCLET +ETL EL++ VEDL EYPNKKSLSQ+ADDLKT +S+A+TNQ TC+DGFSHD 
Sbjct: 139 LHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDD 198

Query: 197 ADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNGIN 256
           A+K VR++L  G +HVEK+CS ALA+IKN+TDTD+      N ++LTE            
Sbjct: 199 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTE-----ETSTVDG 258

Query: 257 WPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVY 316
           WP W+S GDRRLLQ SS+ TPNAVVAADGSG+F+TV  AV AAP   ++RYIIRIKAGVY
Sbjct: 259 WPAWLSPGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVY 318

Query: 317 RENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNT 376
           RENV V    +NIMF GDGRT TIITG+RNVVDGSTTF SAT A VGE FLARD+TFQNT
Sbjct: 319 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNT 378

Query: 377 AGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAV 436
           AGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I G+VDFIFGNAAAV
Sbjct: 379 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 438

Query: 437 IQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRP 496
           +Q+CDIHAR+PN GQ+NMVTAQGRTDPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRP
Sbjct: 439 LQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 498

Query: 497 WQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKV 556
           W+ YSRTV+M +SI+++I PAGWH WDGNFAL+TLFY E+QN+G GA TS RV WKG++V
Sbjct: 499 WKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRV 558

Query: 557 ITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           IT+A EA+AFT G+FI+G +WL STGFPFSLGL
Sbjct: 559 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of HG10002412 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 700.3 bits (1806), Expect = 1.8e-200
Identity = 373/578 (64.53%), Postives = 438/578 (75.78%), Query Frame = 0

Query: 22  KLAKLLILAMSAALL----LAGV---------------SSDEHVRSTCAVTLYPELCETT 81
           K  KL++L+ + ALL    +AG+               SS   +RS+C+ T YPELC + 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 82  ISTA--VASRSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGE 141
           + TA  V   S K+ IEASVN+TI AV+ NY ++KKL+K  K LT REK AL+DCLET +
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 142 ETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESL 201
           ETL EL+E VEDLH YP KK+L ++A DLKT +SSAITNQETC+DGFSHD ADK+VR++L
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 202 EAGLIHVEKLCSIALALIKNLTDTDIANHN-----NLNRKQLTEINN---------GRSD 261
             G IHVE +CS ALA+IKN+TDTDIAN         N ++L E N          G  +
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 262 DNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRI 321
            +   WP W+S GDRRLLQ S     +A VAADGSG F+TV  AV AAP  S++RY+I I
Sbjct: 256 LDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 315

Query: 322 KAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDL 381
           KAGVYRENV V   K+NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAVGERFLARD+
Sbjct: 316 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 375

Query: 382 TFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFG 441
           TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHSNRQF+VKC+I G+VDFIFG
Sbjct: 376 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 435

Query: 442 NAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPT 501
           NAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGIVIQKCRIG TSDL+ V  +FPT
Sbjct: 436 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 495

Query: 502 FLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTW 561
           +LGRPW+ YS+TV+M ++IS+VI P GW  W G FAL+TL Y EY N G GA T+ RV W
Sbjct: 496 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 555

Query: 562 KGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           +G+KVIT AAEA+ +TAG FI GG WLSSTGFPFSLGL
Sbjct: 556 RGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of HG10002412 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 3.1e-200
Identity = 371/573 (64.75%), Postives = 445/573 (77.66%), Query Frame = 0

Query: 17  SISNI-KLAKLLILAMSAALL--------LAGVSSDEH------------VRSTCAVTLY 76
           +ISNI K  K L LA+ A LL        +AGV+S ++            ++S+C+ T Y
Sbjct: 19  NISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRY 78

Query: 77  PELCETTISTAVAS----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIA 136
           P+LC + I+    +     S K+ IE S+NIT  AV+ NY  I+KL+K   NLTKREK+A
Sbjct: 79  PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKR-TNLTKREKVA 138

Query: 137 LNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDK 196
           L+DCLET +ETL EL++ VEDL EYPNKKSLSQ+ADDLKT +S+A+TNQ TC+DGFSHD 
Sbjct: 139 LHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDD 198

Query: 197 ADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNGIN 256
           A+K VR++L  G +HVEK+CS ALA+IKN+TDTD+      N ++L E            
Sbjct: 199 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIE-----ETSTVDG 258

Query: 257 WPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVY 316
           WP W+S GDRRLLQ SS+ TPN VVAADGSG+F+TV  +V AAP   ++RYIIRIKAGVY
Sbjct: 259 WPAWLSTGDRRLLQ-SSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVY 318

Query: 317 RENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNT 376
           RENV V    +NIMF GDGRT TIITG+RNVVDGSTTF SATVA VGE FLARD+TFQNT
Sbjct: 319 RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNT 378

Query: 377 AGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAV 436
           AGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I G+VDFIFGNAAAV
Sbjct: 379 AGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAV 438

Query: 437 IQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRP 496
           +Q+CDIHAR+PN GQ+NMVTAQGR DPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRP
Sbjct: 439 LQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRP 498

Query: 497 WQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKV 556
           W+ YSRTV+M +SI++VI PAGWH WDGNFAL+TLFY E+QN G GA TS RV WKG++V
Sbjct: 499 WKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRV 558

Query: 557 ITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           IT+A EA+AFT G+FI+G +WL STGFPFSLGL
Sbjct: 559 ITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of HG10002412 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 681.4 bits (1757), Expect = 8.7e-195
Identity = 355/538 (65.99%), Postives = 418/538 (77.70%), Query Frame = 0

Query: 40  VSSDEH--VRSTCAVTLYPELCETTISTAVASR--SSKEAIEASVNITIEAVKDNYKSIK 99
           +SS  H  ++S C+ TLYPELC + ++        S KE IEAS+N+T +AVK NY ++K
Sbjct: 54  LSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVK 113

Query: 100 KLVKTLKNLTKREKIALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSS 159
           KL+   K LT RE  AL+DCLET +ETL EL+  VEDLH+YP +KSL ++ADDLKT +SS
Sbjct: 114 KLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISS 173

Query: 160 AITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIAN------- 219
           AITNQ TC+DGFS+D AD+KVR++L  G +HVE +CS ALA+IKN+T+TDIAN       
Sbjct: 174 AITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKS 233

Query: 220 --HNNLNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRT 279
               N N ++L E+  G  D +G  WP W+S GDRRLLQ S+    +A VA DGSGDF T
Sbjct: 234 STFTNNNNRKLKEV-TGDLDSDG--WPKWLSVGDRRLLQGSTIKA-DATVADDGSGDFTT 293

Query: 280 VLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGS 339
           V  AV AAP +S++R++I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGS
Sbjct: 294 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 353

Query: 340 TTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 399
           TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 354 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 413

Query: 400 SNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 459
           SNRQF+VKC I G+VDFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 414 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 473

Query: 460 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTL 519
           Q CRIG TSDL  V   FPT+LGRPW+ YSRTV+M + IS+VI P GWH W G+FALDTL
Sbjct: 474 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 533

Query: 520 FYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
            Y EY N G GA T+ RV WKGYKVIT+  EA+ FTAG FI GG WL+STGFPFSL L
Sbjct: 534 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of HG10002412 vs. ExPASy TrEMBL
Match: A0A5A7UEV0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001090 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.8e-265
Identity = 477/558 (85.48%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 11  AAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCETTISTAVASR 70
           AAG P S ++     LL+ A+S  LLLA VSSD+HVRS CA+TLYPELCETTISTAV S 
Sbjct: 9   AAGVPFSCNS--KTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTISTAVGS- 68

Query: 71  SSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGEETLRELYEVV 130
           SSKEAIEASVNITI AV DNY+ +KKL+KT  +LTKREKIAL+DCLETGEETLRELYEVV
Sbjct: 69  SSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRELYEVV 128

Query: 131 EDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKL 190
           ED++EYPNKKSLS+YADDLKT LSSAITNQETC+DGFSHDKADKKVRESL+ GLIH+EKL
Sbjct: 129 EDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLIHIEKL 188

Query: 191 CSIALALIKNLTDTDIANHNN----LNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQA 250
           CSIALALIKNLTDTDIAN NN    L+RKQL E N     D+ INWP+WMS  DRRLLQA
Sbjct: 189 CSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNT----DDRINWPDWMSPNDRRLLQA 248

Query: 251 SSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMF 310
           SS ATP+ VVAADGSGDFRT+ EAV AAP+RSSQRYIIRIKAGVYRENVNV SSKRNIMF
Sbjct: 249 SSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRNIMF 308

Query: 311 WGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGS 370
           WGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGS
Sbjct: 309 WGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGS 368

Query: 371 DLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQ 430
           DLSAFYRCDMLAYQDTLYVHSNRQFYV+CII+G++DFIFGNAAAVIQDCDIHARRPNPGQ
Sbjct: 369 DLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQ 428

Query: 431 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSIS 490
           RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVM TSIS
Sbjct: 429 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSIS 488

Query: 491 NVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNF 550
           NVIDPAGWHVWDGNFALDTLFYAEYQN+G GADTSRRV WKG++V+T AAEAEAFTAGNF
Sbjct: 489 NVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTAGNF 548

Query: 551 ISGGTWLSSTGFPFSLGL 565
           I GGTWLSSTGFPFSLGL
Sbjct: 549 IGGGTWLSSTGFPFSLGL 559

BLAST of HG10002412 vs. ExPASy TrEMBL
Match: A0A1S3CCA5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.8e-265
Identity = 477/558 (85.48%), Postives = 509/558 (91.22%), Query Frame = 0

Query: 11  AAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCETTISTAVASR 70
           AAG P S ++     LL+ A+S  LLLA VSSD+HVRS CA+TLYPELCETTISTAV S 
Sbjct: 9   AAGVPFSCNS--KTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTISTAVGS- 68

Query: 71  SSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGEETLRELYEVV 130
           SSKEAIEASVNITI AV DNY+ +KKL+KT  +LTKREKIAL+DCLETGEETLRELYEVV
Sbjct: 69  SSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRELYEVV 128

Query: 131 EDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKL 190
           ED++EYPNKKSLS+YADDLKT LSSAITNQETC+DGFSHDKADKKVRESL+ GLIH+EKL
Sbjct: 129 EDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLIHIEKL 188

Query: 191 CSIALALIKNLTDTDIANHNN----LNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQA 250
           CSIALALIKNLTDTDIAN NN    L+RKQL E N     D+ INWP+WMS  DRRLLQA
Sbjct: 189 CSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNT----DDRINWPDWMSPNDRRLLQA 248

Query: 251 SSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMF 310
           SS ATP+ VVAADGSGDFRT+ EAV AAP+RSSQRYIIRIKAGVYRENVNV SSKRNIMF
Sbjct: 249 SSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRNIMF 308

Query: 311 WGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGS 370
           WGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGS
Sbjct: 309 WGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGS 368

Query: 371 DLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQ 430
           DLSAFYRCDMLAYQDTLYVHSNRQFYV+CII+G++DFIFGNAAAVIQDCDIHARRPNPGQ
Sbjct: 369 DLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQ 428

Query: 431 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSIS 490
           RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVM TSIS
Sbjct: 429 RNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSIS 488

Query: 491 NVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNF 550
           NVIDPAGWHVWDGNFALDTLFYAEYQN+G GADTSRRV WKG++V+T AAEAEAFTAGNF
Sbjct: 489 NVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTAGNF 548

Query: 551 ISGGTWLSSTGFPFSLGL 565
           I GGTWLSSTGFPFSLGL
Sbjct: 549 IGGGTWLSSTGFPFSLGL 559

BLAST of HG10002412 vs. ExPASy TrEMBL
Match: A0A0A0LCR6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1)

HSP 1 Score: 918.7 bits (2373), Expect = 1.2e-263
Identity = 473/570 (82.98%), Postives = 510/570 (89.47%), Query Frame = 0

Query: 1   MAQTEDSTAAAAGSPISISNIKLAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCE 60
           M++ +D+ AA     +  S      LL+ A+S  LLLA VSSD+HV S CA+TLYPELCE
Sbjct: 1   MSKPDDTPAAG----VPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCE 60

Query: 61  TTISTAVASRSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGE 120
           TTISTAV S SSKEAIEASVNITI AVKDNYK ++KL+KT+KNLTKR+KIA +DCLETGE
Sbjct: 61  TTISTAVGS-SSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGE 120

Query: 121 ETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESL 180
           ETLRELYEVVED++EYP KKSLS+YADDLKT LSS ITNQETCVDGFSHDK DKKVRESL
Sbjct: 121 ETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESL 180

Query: 181 EAGLIHVEKLCSIALALIKNLTDTDIANHNN------LNRKQLTEINNGRSDDNGINWPN 240
           + GLIH+EKLCSIALALIKNLTDTDIAN NN      LNRKQL E    +  ++GI WP+
Sbjct: 181 KEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEE----KKMEDGIKWPD 240

Query: 241 WMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYREN 300
           WMS  DRRLLQASS ATP+ VVAADGSGDFRT+ EAV AAP+RSS+RYIIRIKAGVYREN
Sbjct: 241 WMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYREN 300

Query: 301 VNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGP 360
           VNV SSKRNIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTAGP
Sbjct: 301 VNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGP 360

Query: 361 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQD 420
           SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV+CII+G++DFIFGNAAAVIQD
Sbjct: 361 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQD 420

Query: 421 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 480
           CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR
Sbjct: 421 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 480

Query: 481 YSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITN 540
           YSRTVVM TSISNVIDPAGWHVWDGNFALDTLFYAEYQN+G GADTSRRV WKG++V+T 
Sbjct: 481 YSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTR 540

Query: 541 AAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           AAEAEAFTAGNFI GGTWLSSTGFPFSLGL
Sbjct: 541 AAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of HG10002412 vs. ExPASy TrEMBL
Match: A0A6J1ITZ4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.9e-262
Identity = 478/575 (83.13%), Postives = 512/575 (89.04%), Query Frame = 0

Query: 1   MAQTEDSTAAAA---GSPISISNIKLAKLLILAMSAALLLAGVSSDEH---VRSTCAVTL 60
           MAQT+DST A A    SP SIS  K   L ILA+SAALLL GVSSD+H   VRSTCAVTL
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISK-KKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTL 60

Query: 61  YPELCETTISTAVAS-----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREK 120
           YPELC++TISTAV +      SSK+ IEASVNITI AVK NYKSIKKLV+T KNLTKREK
Sbjct: 61  YPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREK 120

Query: 121 IALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSH 180
           IAL+DCLETGEETLRELYEVVEDL+EYP KKSL QYADDLKT LSSAITNQETCVDGFSH
Sbjct: 121 IALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 180

Query: 181 DKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNG 240
           D+ADKK+RESL AG IHVEKLCS+ALALIKNLTDTDIANH  L    +   +   S+   
Sbjct: 181 DRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE--- 240

Query: 241 INWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAG 300
            NWP+WMS GDR+LLQASS ATP+ VVAADGSGDF+TV  AV AAP+RSS+RYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQ 360
           VYRENVNVPSSK NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAA 420
           NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CII+G+VDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGY 540
           RPWQRYSRTVVM TSISNVIDPAGWH+WDGNFALDTLFYAE+QN+G GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGY 540

Query: 541 KVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           +VI++AAEAE FTAG FISGGTWLSSTGFPFSLGL
Sbjct: 541 RVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

BLAST of HG10002412 vs. ExPASy TrEMBL
Match: A0A6J1GV42 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 3.8e-262
Identity = 475/575 (82.61%), Postives = 513/575 (89.22%), Query Frame = 0

Query: 1   MAQTEDSTAAAA---GSPISISNIKLAKLLILAMSAALLLAGVSSDEH---VRSTCAVTL 60
           MAQT+DST A A   G P SISN K   L I+ +SAALLLAGVSS++H   VRSTCAVTL
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISN-KKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTL 60

Query: 61  YPELCETTISTAVAS-----RSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREK 120
           YPELC++TISTAV +      SSK+ IEASVNITI AVK NYKSIKKL++T K+LTKREK
Sbjct: 61  YPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREK 120

Query: 121 IALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSH 180
           IAL+DCLETGEETLRELYEVVEDL+EYP KKSL QYADDLKT LSSAITNQETCVDGFSH
Sbjct: 121 IALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 180

Query: 181 DKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSDDNG 240
           D+ADKKVRESL AG  HVEKLCS+ALALIKNLTDTDIANH N+  + +          + 
Sbjct: 181 DRADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAM--------SE 240

Query: 241 INWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAG 300
            NWP+WMS GDR+LLQASS ATP+AVVAADGSGDF+TV  AV AAP+RSS+RYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDLTFQ 360
           VYRENVNVPSSK NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAA 420
           NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CII+G+VDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTWKGY 540
           RPWQRYSRTVVM TSISNVIDPAGWH+WDGNFALDTLFYAEYQNNG GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKGY 540

Query: 541 KVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           ++I++AAEAE+FTAG FISGGTWLSSTGFPFSLGL
Sbjct: 541 RIISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of HG10002412 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 700.3 bits (1806), Expect = 1.3e-201
Identity = 373/578 (64.53%), Postives = 438/578 (75.78%), Query Frame = 0

Query: 22  KLAKLLILAMSAALL----LAGV---------------SSDEHVRSTCAVTLYPELCETT 81
           K  KL++L+ + ALL    +AG+               SS   +RS+C+ T YPELC + 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 82  ISTA--VASRSSKEAIEASVNITIEAVKDNYKSIKKLVKTLKNLTKREKIALNDCLETGE 141
           + TA  V   S K+ IEASVN+TI AV+ NY ++KKL+K  K LT REK AL+DCLET +
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 142 ETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESL 201
           ETL EL+E VEDLH YP KK+L ++A DLKT +SSAITNQETC+DGFSHD ADK+VR++L
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 202 EAGLIHVEKLCSIALALIKNLTDTDIANHN-----NLNRKQLTEINN---------GRSD 261
             G IHVE +CS ALA+IKN+TDTDIAN         N ++L E N          G  +
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 262 DNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYIIRI 321
            +   WP W+S GDRRLLQ S     +A VAADGSG F+TV  AV AAP  S++RY+I I
Sbjct: 256 LDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPENSNKRYVIHI 315

Query: 322 KAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDL 381
           KAGVYRENV V   K+NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAVGERFLARD+
Sbjct: 316 KAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLARDI 375

Query: 382 TFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDFIFG 441
           TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHSNRQF+VKC+I G+VDFIFG
Sbjct: 376 TFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDFIFG 435

Query: 442 NAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPT 501
           NAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGIVIQKCRIG TSDL+ V  +FPT
Sbjct: 436 NAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGSFPT 495

Query: 502 FLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRRVTW 561
           +LGRPW+ YS+TV+M ++IS+VI P GW  W G FAL+TL Y EY N G GA T+ RV W
Sbjct: 496 YLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANRVKW 555

Query: 562 KGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
           +G+KVIT AAEA+ +TAG FI GG WLSSTGFPFSLGL
Sbjct: 556 RGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of HG10002412 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 681.4 bits (1757), Expect = 6.2e-196
Identity = 355/538 (65.99%), Postives = 418/538 (77.70%), Query Frame = 0

Query: 40  VSSDEH--VRSTCAVTLYPELCETTISTAVASR--SSKEAIEASVNITIEAVKDNYKSIK 99
           +SS  H  ++S C+ TLYPELC + ++        S KE IEAS+N+T +AVK NY ++K
Sbjct: 54  LSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVK 113

Query: 100 KLVKTLKNLTKREKIALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSS 159
           KL+   K LT RE  AL+DCLET +ETL EL+  VEDLH+YP +KSL ++ADDLKT +SS
Sbjct: 114 KLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISS 173

Query: 160 AITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIAN------- 219
           AITNQ TC+DGFS+D AD+KVR++L  G +HVE +CS ALA+IKN+T+TDIAN       
Sbjct: 174 AITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKS 233

Query: 220 --HNNLNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVAADGSGDFRT 279
               N N ++L E+  G  D +G  WP W+S GDRRLLQ S+    +A VA DGSGDF T
Sbjct: 234 STFTNNNNRKLKEV-TGDLDSDG--WPKWLSVGDRRLLQGSTIKA-DATVADDGSGDFTT 293

Query: 280 VLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGS 339
           V  AV AAP +S++R++I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGS
Sbjct: 294 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 353

Query: 340 TTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 399
           TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 354 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 413

Query: 400 SNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 459
           SNRQF+VKC I G+VDFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 414 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 473

Query: 460 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTL 519
           Q CRIG TSDL  V   FPT+LGRPW+ YSRTV+M + IS+VI P GWH W G+FALDTL
Sbjct: 474 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 533

Query: 520 FYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPFSLGL 565
            Y EY N G GA T+ RV WKGYKVIT+  EA+ FTAG FI GG WL+STGFPFSL L
Sbjct: 534 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of HG10002412 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-116
Identity = 254/547 (46.44%), Postives = 348/547 (63.62%), Query Frame = 0

Query: 23  LAKLLILAMSAALLLAGVSSDEHVRSTCAVTLYPELCETTISTAVASRSSKEAIEAS--- 82
           +   +IL +  A  ++G +  + V++ C+ T  P+ CE  ++      S+ E I++    
Sbjct: 8   IINFVILCILVASTVSGYNQKD-VKAWCSQTPNPKPCEYFLT----HNSNNEPIKSESEF 67

Query: 83  VNITIEAVKDNYKSIKKLVKTL--KNLTKREKIALNDCLETGEETLRELYEVVEDLHEYP 142
           + I+++ V D     K    TL  K    REK A  DC++  + T+ ++ E ++     P
Sbjct: 68  LKISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD-----P 127

Query: 143 NKKSLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALAL 202
           N K       D +T+LS+A+TN +TC  GF            LE G      +  I L L
Sbjct: 128 NVKCSKL---DAQTWLSTALTNLDTCRAGF------------LELG------VTDIVLPL 187

Query: 203 IKNLTDTDIANHNNLNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVA 262
           + N     + N   +N+      N    + +G  +P+W+  GDR+LLQ SS    NAVVA
Sbjct: 188 MSNNVSNLLCNTLAINK---VPFNYTPPEKDG--FPSWVKPGDRKLLQ-SSTPKDNAVVA 247

Query: 263 ADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIIT 322
            DGSG+F+T+ EA+ AA    S R++I +K GVY EN+ +   K+N+M  GDG   TIIT
Sbjct: 248 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIIT 307

Query: 323 GNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDML 382
           G+++V  G+TTFNSATVAAVG+ F+AR +TF+NTAG S  QAVALR GSDLS FY+C   
Sbjct: 308 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 367

Query: 383 AYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTD 442
           AYQDTLYVHSNRQFY  C + G+VDFIFGNAAAV+Q+C+I ARRP   + N +TAQGR+D
Sbjct: 368 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPR-SKTNTITAQGRSD 427

Query: 443 PNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVW 502
           PNQNTGI+I   R+   SDLRPV+ +  T+LGRPW++YSRTV M TS+ ++IDP GW  W
Sbjct: 428 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 487

Query: 503 DGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTG 562
           DGNFAL TLFYAE+QN GPGA TS RVTW G++V+ +A+EA  FT G F++GG+W+ S+ 
Sbjct: 488 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS- 511

Query: 563 FPFSLGL 565
            PF+ GL
Sbjct: 548 VPFTSGL 511

BLAST of HG10002412 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 414.8 bits (1065), Expect = 1.1e-115
Identity = 224/457 (49.02%), Postives = 294/457 (64.33%), Query Frame = 0

Query: 107 REKIALNDCLETGEETLRELYEVVEDLHEYPNKKSLSQYADDLKTFLSSAITNQETCVDG 166
           R+K    DC E  + +   +   +E+L       +L  Y+ ++ T+LSS +TN  TC++ 
Sbjct: 118 RDKAGFADCEEMMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLES 177

Query: 167 FSHDKADKKVRESLEAGLIHVEKLCSIALALIKNLTDTDIANHNNLNRKQLTEINNGRSD 226
            S    + K  + ++  L  +     +ALA+  ++            R  L  I + R  
Sbjct: 178 ISDVSVNSK--QIVKPQLEDLVSRARVALAIFVSVLPA---------RDDLKMIISNR-- 237

Query: 227 DNGINWPNWMSHGDRRLLQASS---AATPNAVVAADGSGDFRTVLEAVVAAPNRSSQRYI 286
                +P+W++  DR+LL++S      T N VVA DG+G F+TV EAV AAP  S+ RY+
Sbjct: 238 -----FPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYV 297

Query: 287 IRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLA 346
           I +K GVY+E +++   K+N+M  GDG+  TIITG+ NV+DGSTTF SATVAA G+ F+A
Sbjct: 298 IYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMA 357

Query: 347 RDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVKCIIIGSVDF 406
           +D+ FQNTAGP+KHQAVALRV +D +   RC + AYQDTLY H+ RQFY    I G+VDF
Sbjct: 358 QDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDF 417

Query: 407 IFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISN 466
           IFGN+A V Q+CDI AR P  GQ+NM+TAQGR D NQNT I IQKC+I  +SDL PV  +
Sbjct: 418 IFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGS 477

Query: 467 FPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVWDGNFALDTLFYAEYQNNGPGADTSRR 526
             TFLGRPW+ YSRTV+M + I N IDPAGW  WDG FAL TL+Y EY N GPGADTS+R
Sbjct: 478 VKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKR 537

Query: 527 VTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTGFPF 561
           V WKG+KVI ++ EAE FT    I GG WL  TG  F
Sbjct: 538 VNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553

BLAST of HG10002412 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 406.8 bits (1044), Expect = 2.9e-113
Identity = 221/487 (45.38%), Postives = 299/487 (61.40%), Query Frame = 0

Query: 85  EAVKDNYKSIKKLVKTLKNLTK-----REKIALNDCLETGEETLRELYEVVEDLHEYPNK 144
           EA K    +I K V  +    K     R   A+ DC++  +    EL  ++    + PN 
Sbjct: 49  EAAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWII-SASQSPNG 108

Query: 145 K--SLSQYADDLKTFLSSAITNQETCVDGFSHDKADKKVRESLEAGLIHVEKLCSIALAL 204
           K  S      DL+T++S+A++NQ+TC+DGF  +  +  +++ +  GL  V       L +
Sbjct: 109 KDNSTGDVGSDLRTWISAALSNQDTCLDGF--EGTNGIIKKIVAGGLSKVGTTVRNLLTM 168

Query: 205 IKNLTDTDIANHNNLNRKQLTEINNGRSDDNGINWPNWMSHGDRRLLQASSAATPNAVVA 264
           +   +         +  + +T+ ++G S      +P+W+  GDR+LLQ  +    +AVVA
Sbjct: 169 VH--SPPSKPKPKPIKAQTMTKAHSGFS-----KFPSWVKPGDRKLLQTDNITVADAVVA 228

Query: 265 ADGSGDFRTVLEAVVAAPNRSSQRYIIRIKAGVYRENVNVPSSKRNIMFWGDGRTTTIIT 324
           ADG+G+F T+ +AV+AAP+ S++RY+I +K GVY ENV +   K NIM  GDG   T+IT
Sbjct: 229 ADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVIT 288

Query: 325 GNRNVVDGSTTFNSATVAAVGERFLARDLTFQNTAGPSKHQAVALRVGSDLSAFYRCDML 384
           GNR+ +DG TTF SAT A  G  F+ARD+TFQNTAGP KHQAVA+R  +DL  FYRC M 
Sbjct: 289 GNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMR 348

Query: 385 AYQDTLYVHSNRQFYVKCIIIGSVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTD 444
            YQDTLY HS RQF+ +CII G+VDFIFG+A AV Q C I A++  P Q+N +TAQGR D
Sbjct: 349 GYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKD 408

Query: 445 PNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMHTSISNVIDPAGWHVW 504
           PN+ TG  IQ   I   +DL   ++   T+LGRPW+ YSRTV M   +S+ I+P GW  W
Sbjct: 409 PNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEW 468

Query: 505 DGNFALDTLFYAEYQNNGPGADTSRRVTWKGYKVITNAAEAEAFTAGNFISGGTWLSSTG 564
           +GNFALDTL+Y EY N+GPGA   RRV W GY V+  +AEA  FT    I G  WL STG
Sbjct: 469 NGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTG 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878011.16.7e-27787.24pectinesterase-like [Benincasa hispida][more]
XP_008460358.15.9e-26585.48PREDICTED: pectinesterase-like [Cucumis melo] >KAA0053588.1 pectinesterase-like ... [more]
XP_004144447.22.5e-26382.98pectinesterase [Cucumis sativus] >KGN58477.1 hypothetical protein Csa_002294 [Cu... [more]
XP_023526589.11.2e-26282.96pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022980581.16.1e-26283.13pectinesterase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q431431.9e-20267.47Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O048867.3e-20265.10Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
O490061.8e-20064.53Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
P839483.1e-20064.75Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Q425348.7e-19565.99Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7UEV02.8e-26585.48Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00109... [more]
A0A1S3CCA52.8e-26585.48Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1[more]
A0A0A0LCR61.2e-26382.98Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1[more]
A0A6J1ITZ42.9e-26283.13Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1[more]
A0A6J1GV423.8e-26282.61Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.11.3e-20164.53pectin methylesterase 3 [more]
AT1G53830.16.2e-19665.99pectin methylesterase 2 [more]
AT2G45220.11.3e-11646.44Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G11580.11.1e-11549.02methylesterase PCR A [more]
AT3G43270.12.9e-11345.38Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 84..104
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 42..563
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 42..563
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..202
e-value: 5.25016E-39
score: 137.955
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..197
e-value: 2.3E-36
score: 136.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 46..197
e-value: 6.5E-26
score: 91.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 26..202
e-value: 3.1E-20
score: 70.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 40..203
e-value: 3.1E-30
score: 107.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..202
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 253..549
e-value: 3.3E-144
score: 479.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 247..564
e-value: 2.7E-125
score: 420.0
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 397..406
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 278..297
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 247..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002412.1HG10002412.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity