HG10002395 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10002395
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cytochrome P450
Location	Chr11: 6328152 .. 6328661 (-)
RNA-Seq Expression	HG10002395
Synteny	HG10002395

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACTCCTTGGTCTTGTTGTGTGGTTGCATGGTTATTAGCCACTTTCTTCCTTCTCTTCTTCCTCTCTCGTCGTCCTCATAACCTCAATCAACCACCAGGACCAAAGCCATGGCCTTTCATTGGAAATCTCCATTTGATTGGCTCTTTACCACATCAATCCATTCATCAACTCTCCAAAAAATATGGCCCCATAATGCAGCTCCGTTTTGGCTCCTTCCCTGTTATTGTTGCATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTACCGCCCCGAAAACGCCGTCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAATACGGCCCATATTGGCGTCAAGCAAGAAAGATATTTTTTATGGGAGATTTTCAGTCCAAAAAGACTCGACTCTCACGAGTACATAAGAAAGCAAGAAATGAATGGTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGATTCAAGAATTGA

mRNA sequence

ATGGAAACTCCTTGGTCTTGTTGTGTGGTTGCATGGTTATTAGCCACTTTCTTCCTTCTCTTCTTCCTCTCTCGTCGTCCTCATAACCTCAATCAACCACCAGGACCAAAGCCATGGCCTTTCATTGGAAATCTCCATTTGATTGGCTCTTTACCACATCAATCCATTCATCAACTCTCCAAAAAATATGGCCCCATAATGCAGCTCCGTTTTGGCTCCTTCCCTGTTATTGTTGCATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTACCGCCCCGAAAACGCCGTCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAATACGGCCCATATTGGCGTCAAGCAAGAAAGATATTTTTTATGGGAGATTTTCAGTCCAAAAAGACTCGACTCTCACGAGTACATAAGAAAGCAAGAAATGAATGGTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGATTCAAGAATTGA

Coding sequence (CDS)

ATGGAAACTCCTTGGTCTTGTTGTGTGGTTGCATGGTTATTAGCCACTTTCTTCCTTCTCTTCTTCCTCTCTCGTCGTCCTCATAACCTCAATCAACCACCAGGACCAAAGCCATGGCCTTTCATTGGAAATCTCCATTTGATTGGCTCTTTACCACATCAATCCATTCATCAACTCTCCAAAAAATATGGCCCCATAATGCAGCTCCGTTTTGGCTCCTTCCCTGTTATTGTTGCATCCTCTGTAGAAATGGCAAAGCTTTTCCTCAAAACTCATGATCTTACCTTCGTTTACCGCCCCGAAAACGCCGTCGGAAAGTATGTAACCTACAACTATTCCAACATCACATGGTCTCAATACGGCCCATATTGGCGTCAAGCAAGAAAGATATTTTTTATGGGAGATTTTCAGTCCAAAAAGACTCGACTCTCACGAGTACATAAGAAAGCAAGAAATGAATGGTTTTCTTCAAGAAATTTACAAGGCTTCTTGCCAAGATTCAAGAATTGA

Protein sequence

METPWSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFFMGDFQSKKTRLSRVHKKARNEWFSSRNLQGFLPRFKN

Homology

[Close]

BLAST of HG10002395 vs. NCBI nr
Match: XP_038904918.1 (LOW QUALITY PROTEIN: trimethyltridecatetraene synthase-like [Benincasa hispida])

HSP 1 Score: 241.9 bits (616), Expect = 4.0e-60
Identity = 114/140 (81.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   METPWSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLS 60
           METP++ CVV    A FFLLFFL R  H LNQPPGPKPWP IGN+HLIGSL HQSIHQLS
Sbjct: 1   METPYTSCVV----ACFFLLFFLFRCRHKLNQPPGPKPWPLIGNIHLIGSLXHQSIHQLS 60

Query: 61  KKYGPIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQY 120
           KKYGPIMQLRFGSFPV+V SSVEMAKLFLKTHDLTFVYRP+NA+GKYVTYNYSNITWSQY
Sbjct: 61  KKYGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLTFVYRPKNAIGKYVTYNYSNITWSQY 120

Query: 121 GPYWRQARKIFFMGDFQSKK 141
           GPYWRQARK+F M  F  K+
Sbjct: 121 GPYWRQARKMFMMELFSPKR 136

[Close]

BLAST of HG10002395 vs. NCBI nr
Match: XP_023524445.1 (cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.2 bits (544), Expect = 9.0e-52
Identity = 103/149 (69.13%), Postives = 116/149 (77.85%), Query Frame = 0


Query: 5   WSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 64
           W     A +     LL  LSRR    N PPGPKPWP IGNLHL+GSLPHQSIHQLSKKYG
Sbjct: 6   WLSHAAAGMPTLAILLLLLSRRLRRPNLPPGPKPWPLIGNLHLMGSLPHQSIHQLSKKYG 65

Query: 65  PIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYW 124
           PIMQLRFGSFPV+V SSV+MAKLFLKTHDL FVYRP+ AVGKY+TYNYSNITWSQYGPYW
Sbjct: 66  PIMQLRFGSFPVVVGSSVQMAKLFLKTHDLNFVYRPKTAVGKYMTYNYSNITWSQYGPYW 125

Query: 125 RQARKIFFMGDFQSKKTRLSR-VHKKARN 153
           RQARKI+ +  F +K+   S  + ++ RN
Sbjct: 126 RQARKIWLLELFSAKRLDSSEYIRREERN 154

[Close]

BLAST of HG10002395 vs. NCBI nr
Match: KAG6606687.1 (Coatomer subunit beta-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-51
Identity = 101/131 (77.10%), Postives = 111/131 (84.73%), Query Frame = 0


Query: 10  VAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQL 69
           +A LL    LL  LSRR    N PPGPKPWP IGNLHL+GSLPHQSIHQLSKKYGPIMQL
Sbjct: 4   LAILLLLLLLLPLLSRRLRRPNLPPGPKPWPVIGNLHLMGSLPHQSIHQLSKKYGPIMQL 63

Query: 70  RFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARK 129
           RFGSFPV+V SSV+MAKLFLKTHDL FVYRP+ AVGKY+TYNYSNITWSQYGPYWRQARK
Sbjct: 64  RFGSFPVVVGSSVQMAKLFLKTHDLNFVYRPKTAVGKYMTYNYSNITWSQYGPYWRQARK 123

Query: 130 IFFMGDFQSKK 141
           I+ +  F +K+
Sbjct: 124 IWLLELFSAKR 134

[Close]

BLAST of HG10002395 vs. NCBI nr
Match: XP_022997924.1 (cytochrome P450 71A1-like [Cucurbita maxima])

HSP 1 Score: 212.2 bits (539), Expect = 3.4e-51
Identity = 102/138 (73.91%), Postives = 112/138 (81.16%), Query Frame = 0


Query: 9   VVAWL------LATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKK 68
           V +WL      +AT  +L  LSRR    N PPGPKPWP IGNLHLIGSLPH+SIHQLSKK
Sbjct: 3   VSSWLSYAAAGMATLAILLLLSRRLRRPNLPPGPKPWPIIGNLHLIGSLPHRSIHQLSKK 62

Query: 69  YGPIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGP 128
           YGPIMQLRFGSFPV+V SSVEMAKLFLKTHDL FV RP+ AVGKY+TYNYSNITWSQYGP
Sbjct: 63  YGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLNFVSRPKTAVGKYITYNYSNITWSQYGP 122

Query: 129 YWRQARKIFFMGDFQSKK 141
           YWRQARKI  +  F +K+
Sbjct: 123 YWRQARKICLLELFSAKR 140

[Close]

BLAST of HG10002395 vs. NCBI nr
Match: XP_022949462.1 (cytochrome P450 71A1-like [Cucurbita moschata])

HSP 1 Score: 210.7 bits (535), Expect = 9.9e-51
Identity = 97/128 (75.78%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFG 72
           +L    LL  LSRR    N PPGPKPWP IGNLHL+GSLPHQSIH+LSKKYGPIMQLRFG
Sbjct: 36  ILLLLLLLLLLSRRLRRPNLPPGPKPWPVIGNLHLMGSLPHQSIHRLSKKYGPIMQLRFG 95

Query: 73  SFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFF 132
           SFPV+V SSV+MAKLFLK+HDL FVYRP+ AVGKY+TYNYSNITWSQYGPYWRQARKI+ 
Sbjct: 96  SFPVVVGSSVQMAKLFLKSHDLNFVYRPKTAVGKYMTYNYSNITWSQYGPYWRQARKIWL 155

Query: 133 MGDFQSKK 141
           +  F +K+
Sbjct: 156 LELFSAKR 163

[Close]

BLAST of HG10002395 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.2e-29
Identity = 65/133 (48.87%), Postives = 83/133 (62.41%), Query Frame = 0


Query: 11  AWLLATFFLLF---FLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIM 70
           A L+ATF   F    L +   N   PPGP PWP IGN H +    H+++  L++KYGPI+
Sbjct: 18  AILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPIL 77

Query: 71  QLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQA 130
            LRFGS P +V SS E AK FLKTHDL F  RP  +VGKY  YN+ +I +S YG +WR+ 
Sbjct: 78  FLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKM 137

Query: 131 RKIFFMGDFQSKK 141
           RKI  +    SK+
Sbjct: 138 RKICVLELLTSKR 150

[Close]

BLAST of HG10002395 vs. ExPASy Swiss-Prot
Match: P37118 (Cytochrome P450 71A2 OS=Solanum melongena OX=4111 GN=CYP71A2 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.6e-27
Identity = 56/130 (43.08%), Postives = 87/130 (66.92%), Query Frame = 0


Query: 4   PWSCCVVAWLLATFFLL---FFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLS 63
           PW   ++   +  F L+   FF + +  N+   P P+  P IGNLH +GSLPH+S+H+LS
Sbjct: 6   PWYSLLIPLFVFIFLLIHHCFFTTSKKQNMLLLPSPRKLPIIGNLHQLGSLPHRSLHKLS 65

Query: 64  KKYGPIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQY 123
           +KYGP+M L FGS PVIVASSV+ A+  +KTHD+ +  RP++++   ++Y   ++ +S +
Sbjct: 66  QKYGPVMLLHFGSKPVIVASSVDAARDIMKTHDVVWASRPKSSIVDRLSYGSKDVGFSPF 125

Query: 124 GPYWRQARKI 131
           G YWR+A+ I
Sbjct: 126 GEYWRRAKSI 135

[Close]

BLAST of HG10002395 vs. ExPASy Swiss-Prot
Match: Q9LTM1 (Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-26
Identity = 62/141 (43.97%), Postives = 83/141 (58.87%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFG 72
           LL   FL+FF    P     PPGP   P IGNLH +G   H+S H+LS+ YGP+M L FG
Sbjct: 9   LLLPLFLIFFKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVMFLHFG 68

Query: 73  SFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFF 132
             PV+V S+ E A+  LKTHDL    RP+    K  +YNY +I ++QYG  WR+ RK+  
Sbjct: 69  VVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAM 128

Query: 133 MGDFQSKKTRLSRVHKKARNE 154
           +  F SKK +  R  ++  +E
Sbjct: 129 LELFSSKKLKAFRYIREEESE 149

[Close]

BLAST of HG10002395 vs. ExPASy Swiss-Prot
Match: A0A068Q6L2 (Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.0e-26
Identity = 66/157 (42.04%), Postives = 90/157 (57.32%), Query Frame = 0


Query: 13  LLATFFLLFF-----LSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIM 72
           LLA F +L +              QPP P   P IGNLH IGS PH+S+  LS+++GP+M
Sbjct: 25  LLAIFLVLLYTWYSSTKTTTQKSTQPPSPPKLPIIGNLHQIGSYPHRSLQALSQRHGPLM 84

Query: 73  QLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQA 132
            L FGS PV+V SS E A+  LKTHDLTF  RP++ + + + YNY ++  + YG YWRQ 
Sbjct: 85  LLHFGSVPVLVVSSAEAAREILKTHDLTFSDRPKSTIFEKLLYNYKDVASAPYGEYWRQV 144

Query: 133 RKIFFMGDFQSKKTRLSRVHKKARNEWFSS--RNLQG 163
           R I  +    +++ R  R     R E   S  RN++G
Sbjct: 145 RSICVLNLLSNRRVRSFR---SVREEETKSMIRNIKG 178

[Close]

BLAST of HG10002395 vs. ExPASy Swiss-Prot
Match: W8JCE8 (Cytochrome P450 71BT1 OS=Catharanthus roseus OX=4058 GN=CYP71BT1 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.4e-26
Identity = 61/132 (46.21%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLI-GSLPHQSIHQLSKKYGPIMQLRF 72
           LL   F+L  LS++   L  PPGP+P P IGNLH I G L H  + ++++ YGP+ +++F
Sbjct: 11  LLLFCFMLLKLSKKYSALKLPPGPRPLPIIGNLHQIGGGLMHHILREMARTYGPLFRIKF 70

Query: 73  GSFPVIVASSVEMAKLFLKTHDLTFVYRPEN-AVGKYVTYNYSNITWSQYGPYWRQARKI 132
           G    IV SS E+AK   +THD+ F YRP+N AV   +TYN+SNI ++ YG YWRQ RKI
Sbjct: 71  GQVSTIVVSSPEIAKEIFRTHDVVFSYRPQNIAVFNVMTYNFSNIAFAPYGNYWRQMRKI 130

Query: 133 FFMGDFQSKKTR 143
             M    + + R
Sbjct: 131 CVMEILGASRVR 142

[Close]

BLAST of HG10002395 vs. ExPASy TrEMBL
Match: A0A6J1KCW6 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111492726 PE=3 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.6e-51
Identity = 102/138 (73.91%), Postives = 112/138 (81.16%), Query Frame = 0


Query: 9   VVAWL------LATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKK 68
           V +WL      +AT  +L  LSRR    N PPGPKPWP IGNLHLIGSLPH+SIHQLSKK
Sbjct: 3   VSSWLSYAAAGMATLAILLLLSRRLRRPNLPPGPKPWPIIGNLHLIGSLPHRSIHQLSKK 62

Query: 69  YGPIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGP 128
           YGPIMQLRFGSFPV+V SSVEMAKLFLKTHDL FV RP+ AVGKY+TYNYSNITWSQYGP
Sbjct: 63  YGPIMQLRFGSFPVVVGSSVEMAKLFLKTHDLNFVSRPKTAVGKYITYNYSNITWSQYGP 122

Query: 129 YWRQARKIFFMGDFQSKK 141
           YWRQARKI  +  F +K+
Sbjct: 123 YWRQARKICLLELFSAKR 140

[Close]

BLAST of HG10002395 vs. ExPASy TrEMBL
Match: A0A6J1GC32 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452798 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 4.8e-51
Identity = 97/128 (75.78%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFG 72
           +L    LL  LSRR    N PPGPKPWP IGNLHL+GSLPHQSIH+LSKKYGPIMQLRFG
Sbjct: 36  ILLLLLLLLLLSRRLRRPNLPPGPKPWPVIGNLHLMGSLPHQSIHRLSKKYGPIMQLRFG 95

Query: 73  SFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFF 132
           SFPV+V SSV+MAKLFLK+HDL FVYRP+ AVGKY+TYNYSNITWSQYGPYWRQARKI+ 
Sbjct: 96  SFPVVVGSSVQMAKLFLKSHDLNFVYRPKTAVGKYMTYNYSNITWSQYGPYWRQARKIWL 155

Query: 133 MGDFQSKK 141
           +  F +K+
Sbjct: 156 LELFSAKR 163

[Close]

BLAST of HG10002395 vs. ExPASy TrEMBL
Match: A0A6J1KHK2 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111493302 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.4e-50
Identity = 98/136 (72.06%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 5   WSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 64
           W     AW+     LL     R  NLN PPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG
Sbjct: 8   WVFYAAAWMATLAILLLSRRLRRPNLNPPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 67

Query: 65  PIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYW 124
           PIMQLRFGSFPV+V SSVEMAK+FLKTHDL FV RP+ A GKY TYNYS+ITWSQYGPYW
Sbjct: 68  PIMQLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 127

Query: 125 RQARKIFFMGDFQSKK 141
           RQARK+  +  F +K+
Sbjct: 128 RQARKMCQLELFSAKR 143

[Close]

BLAST of HG10002395 vs. ExPASy TrEMBL
Match: A0A6J1GC60 (cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452799 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.4e-50
Identity = 97/136 (71.32%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 5   WSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 64
           W     AW+     LL     R  NLN PPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG
Sbjct: 8   WVFYAAAWMATLAILLLSRRLRRPNLNPPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 67

Query: 65  PIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYW 124
           PIMQLRFGSFPV+V SSVEMAK+FLKTHDL FV RP+ A GKY TYNYS+ITWSQYGPYW
Sbjct: 68  PIMQLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 127

Query: 125 RQARKIFFMGDFQSKK 141
           RQARK+  +  F +++
Sbjct: 128 RQARKMCLLELFSARR 143

[Close]

BLAST of HG10002395 vs. ExPASy TrEMBL
Match: A0A6J1IRR8 (cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 2.0e-49
Identity = 97/136 (71.32%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 5   WSCCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYG 64
           W     AWL    FLL     R  NLN PPGPKPWP IGNL LIGSLPHQSIH LSKKYG
Sbjct: 10  WVSYAAAWLAILAFLLLSRRLRRRNLNLPPGPKPWPLIGNLDLIGSLPHQSIHHLSKKYG 69

Query: 65  PIMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYW 124
           PIM LRFGSFPV+V SSVEMAK+FLKTHDL FV RP+ A GKY TYNYS+ITWSQYGPYW
Sbjct: 70  PIMHLRFGSFPVVVGSSVEMAKIFLKTHDLNFVSRPKTAAGKYTTYNYSDITWSQYGPYW 129

Query: 125 RQARKIFFMGDFQSKK 141
           RQARK+  M  F +K+
Sbjct: 130 RQARKMCLMELFSAKR 145

[Close]

BLAST of HG10002395 vs. TAIR 10
Match: AT3G26200.1 (cytochrome P450, family 71, subfamily B, polypeptide 22 )

HSP 1 Score: 121.3 bits (303), Expect = 7.4e-28
Identity = 62/141 (43.97%), Postives = 83/141 (58.87%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFG 72
           LL   FL+FF    P     PPGP   P IGNLH +G   H+S H+LS+ YGP+M L FG
Sbjct: 9   LLLPLFLIFFKKLSPSKGKLPPGPLGLPIIGNLHQLGKSLHRSFHKLSQNYGPVMFLHFG 68

Query: 73  SFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFF 132
             PV+V S+ E A+  LKTHDL    RP+    K  +YNY +I ++QYG  WR+ RK+  
Sbjct: 69  VVPVVVVSTREAAEEVLKTHDLETCTRPKLTATKLFSYNYKDIGFAQYGDDWREMRKLAM 128

Query: 133 MGDFQSKKTRLSRVHKKARNE 154
           +  F SKK +  R  ++  +E
Sbjct: 129 LELFSSKKLKAFRYIREEESE 149

[Close]

BLAST of HG10002395 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 116.3 bits (290), Expect = 2.4e-26
Identity = 59/138 (42.75%), Postives = 79/138 (57.25%), Query Frame = 0


Query: 12  WLLATFFL------LFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGP 71
           W L   FL         L +R  +  +PP P  +P IGNLH +G LPHQS+  LSKKYGP
Sbjct: 5   WFLPLLFLSCLLLAALRLKKRRQHQRKPPSPPGFPIIGNLHQLGELPHQSLWSLSKKYGP 64

Query: 72  IMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWR 131
           +M L+FGS P +V SS E AK  LK HDL    RP  A  + ++YNY +I +S +  YW+
Sbjct: 65  VMLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWK 124

Query: 132 QARKIFFMGDFQSKKTRL 144
           + R++     F  K+  L
Sbjct: 125 ELRRMCVQELFSPKQVHL 142

[Close]

BLAST of HG10002395 vs. TAIR 10
Match: AT3G26190.1 (cytochrome P450, family 71, subfamily B, polypeptide 21 )

HSP 1 Score: 116.3 bits (290), Expect = 2.4e-26
Identity = 60/141 (42.55%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 13  LLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPIMQLRFG 72
           LL   FL+F+    P     PPGP   P IGNLH +G   H+S ++LS++YGP+M LRFG
Sbjct: 9   LLLPLFLVFYKRLLPSKGKLPPGPISLPIIGNLHQLGKSLHRSFYKLSQEYGPVMFLRFG 68

Query: 73  SFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQARKIFF 132
             PV+V S+ E A+  LKTHDL    RP+ +     TYN+ +I ++QYG  WR+ RK+  
Sbjct: 69  VVPVVVFSTKEAAEEVLKTHDLETCTRPKLSATGLFTYNFKDIGFAQYGEDWREMRKLAM 128

Query: 133 MGDFQSKKTRLSRVHKKARNE 154
           +  F SKK +  R  ++  +E
Sbjct: 129 LELFSSKKLKAFRYIREEESE 149

[Close]

BLAST of HG10002395 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 114.4 bits (285), Expect = 9.0e-26
Identity = 57/139 (41.01%), Postives = 79/139 (56.83%), Query Frame = 0


Query: 7   CCVVAWLLATFFLLFFLSRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGPI 66
           C  +  LL     +F    +    N PP P   P IGNLH +  LPH+  H+LS KYGP+
Sbjct: 6   CFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYGPL 65

Query: 67  MQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWRQ 126
           + LR GS PV+V SS E A+  LKT+DL    RP+      ++Y + +IT++ YG YWR+
Sbjct: 66  VFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEYWRE 125

Query: 127 ARKIFFMGDFQSKKTRLSR 146
            RK+  +  F SKK +  R
Sbjct: 126 VRKLAVIELFSSKKVQSFR 144

[Close]

BLAST of HG10002395 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 114.4 bits (285), Expect = 9.0e-26
Identity = 59/135 (43.70%), Postives = 78/135 (57.78%), Query Frame = 0


Query: 12  WLLATFFLLFFL------SRRPHNLNQPPGPKPWPFIGNLHLIGSLPHQSIHQLSKKYGP 71
           W L+  FL   L       +R  N  QPP P  +P IGNLH +G LPHQS+  LSK YGP
Sbjct: 5   WFLSLLFLCCILLAAFKHKKRRTNQQQPPSPPGFPIIGNLHQLGELPHQSLWSLSKTYGP 64

Query: 72  IMQLRFGSFPVIVASSVEMAKLFLKTHDLTFVYRPENAVGKYVTYNYSNITWSQYGPYWR 131
           +M L+ GS P +V SS E AK  LK +DL    RP  A  K ++YNY +I +S +  YW+
Sbjct: 65  VMLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWK 124

Query: 132 QARKIFFMGDFQSKK 141
           + R+I     F +K+
Sbjct: 125 ELRRICVQELFSAKR 139

The following BLAST results are available for this feature:

BLAST of HG10002395 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904918.1	4.0e-60	81.43	LOW QUALITY PROTEIN: trimethyltridecatetraene synthase-like [Benincasa hispida]	[more]
XP_023524445.1	9.0e-52	69.13	cytochrome P450 71A1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6606687.1	1.5e-51	77.10	Coatomer subunit beta-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022997924.1	3.4e-51	73.91	cytochrome P450 71A1-like [Cucurbita maxima]	[more]
XP_022949462.1	9.9e-51	75.78	cytochrome P450 71A1-like [Cucurbita moschata]	[more]

BLAST of HG10002395 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK4	2.2e-29	48.87	Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1	[more]
P37118	4.6e-27	43.08	Cytochrome P450 71A2 OS=Solanum melongena OX=4111 GN=CYP71A2 PE=2 SV=1	[more]
Q9LTM1	1.0e-26	43.97	Cytochrome P450 71B22 OS=Arabidopsis thaliana OX=3702 GN=CYP71B22 PE=2 SV=1	[more]
A0A068Q6L2	1.0e-26	42.04	Cytochrome P450 736A117 OS=Prunus mume OX=102107 GN=CYP736A117 PE=2 SV=1	[more]
W8JCE8	1.4e-26	46.21	Cytochrome P450 71BT1 OS=Catharanthus roseus OX=4058 GN=CYP71BT1 PE=2 SV=1	[more]

BLAST of HG10002395 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KCW6	1.6e-51	73.91	cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111492726 PE=3 SV=1	[more]
A0A6J1GC32	4.8e-51	75.78	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452798 PE=3 SV=...	[more]
A0A6J1KHK2	1.4e-50	72.06	cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111493302 PE=3 SV=1	[more]
A0A6J1GC60	1.4e-50	71.32	cytochrome P450 71A1-like OS=Cucurbita moschata OX=3662 GN=LOC111452799 PE=3 SV=...	[more]
A0A6J1IRR8	2.0e-49	71.32	cytochrome P450 71A1-like OS=Cucurbita maxima OX=3661 GN=LOC111479921 PE=3 SV=1	[more]

BLAST of HG10002395 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26200.1	7.4e-28	43.97	cytochrome P450, family 71, subfamily B, polypeptide 22	[more]
AT3G26330.1	2.4e-26	42.75	cytochrome P450, family 71, subfamily B, polypeptide 37	[more]
AT3G26190.1	2.4e-26	42.55	cytochrome P450, family 71, subfamily B, polypeptide 21	[more]
AT1G13080.1	9.0e-26	41.01	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT2G02580.1	9.0e-26	43.70	cytochrome P450, family 71, subfamily B, polypeptide 9	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..144 e-value: 1.6E-16 score: 60.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 20..161 e-value: 2.0E-28 score: 101.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 25..134
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 6..141
None	No IPR available	PANTHER	PTHR24281:SF126	CYTOCHROME P450 71A1-LIKE	coord: 6..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10002395.1	HG10002395.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen