HG10002292 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002292
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMalic enzyme
LocationChr11: 5261252 .. 5266009 (+)
RNA-Seq ExpressionHG10002292
SyntenyHG10002292
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTTCTGGAGCTTGCTCCACTCCCTCCGGCACCGGGAGACGGCAAGCTGTGGTTCTGAAGGTGGTGTCGATGGATTCGAATGGTCGAGCCGGCGAGCGAAGCGGCAGTGTTGTGGTGGAGAACAACACTGTGAAAGAAATTGCCAAACCTCCCTCGCCGGTGGTTGAAGTGGAACCCAAATCCACCGCCGGCGCCTGGGCTCAGGATGCGTATGTTGAAGATTCCGCCGCCGCCACGGAGGAACAAATCATGACACCTTGGAGCGTCTCTGTTGCAAGGTGAAGATCACGATCCCCATCTTTCCTATTCATCAAGATTTTCTTTTTCTTCTATTTTTAGATTTGATTGTGTTGTTTATTGACATTCTTTTTTTAACCTTGTGGCATCTTAATCATGACCTTAATTTATTTTTAAACGTTATAGGAATTAACTGATTGCAATTTGCATCATATTATATTTGAATGTGATGCTTAATATATTGGCTTTTTCCTTATGGGGGTTCTATTTTACTATCCTCTTTTAATATTTATCTTAATTTTATATCTTGTGTGCTTCAACCTGAGGATTCATGCATGATGACTGAAGCTGTTATTATTGATTCAAGCAGATCTTGTTTTACTCAGTGGCTTTGTTGGTATTCTACACATATTGTAGTGATCCAGCTTGCAGTTGGAAAAAGCGAGGAGATTGACATAATTTTCTCCTTCTTTGGAAAATGATGATAACCTGGAAGTCCATTTTTGTGTTCAACCAAACCATTTTAATCTGTTCTATTGTGGAAAAGAATTGACATAAGTTCGGCTTTGGGACTGTTACTTTGATGTATAACCTCCTCCCTTTATCTCATTTCCAATCTAGATCGATCAATTATAAACTTCTGAATTGAATAATGTCTTTTTCATGATCCTAGTCCTTTGTTCTTGATAATTTGTAGAGCAATATGTTATTTGCAATTGTATATTCCATAAAGTTGCTGATTTGTGATTTACTGCTCTGACTTATTTGAGCTCAACCTTTGTGAAACTTCTTTTCCAGTTTACATGTTACTGCTTTCTCGTTTCTGCTGTTTTATATCTTAACCTTTGATAGCTAATTGTAGTTTTTGTGTTGGTTTTTGCTGAAACCTTAGTGGATATAATTTATTGCGAGACCCACGGCACAACAAAGGGCTTGCGTTTACTGAAAAAGAAAGAGACGCTCACTACTTGCGCGGTCTTCTACCCCCGACAATTGTTGCTCAAAATCTTCAGGTTTGTCTTCACTTTGGATACTGTTCAGTCCCAGTATAAGTTTTACAATCTAATTCTTTATTATGAAAACTATTTGAACTACTTTCCAGGTAAAGAAGATGTTGCACAATATCCGTCAATATCAAGTACCATTGCAGAAGTACATGGCAATGATGGATCTTCAGGTAGCCATTGTTGATCTCATCTTTTAAGTTCTAGGACTTCTAAATGATTCTTGGTTATGATTTTTGGATTTCATGATCTTGAATACAAAATTGTGAACCTTGTTAAACCAACGTCTGTTCATAATTTATGCTTCATCCCTTGCAAATACATTGGTGAAACTTGTTTTGTGATAAATTCAACAAGTTTCTCTCAAATTGATACTCTGGTGACCAGATTCAGTGAACTGTGTCTTACAACTGACTTTTGCTGTACCCTTTTTCAGGAGAGGAATGAGAAGTTGTTTTACAAGCTCCTTATAGATCATGTAGAAGAATTGCTCCCAGTTGTCTATACACCCACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCAACCACAGGGTCTATATATCAGTTTGAGAGAAAAGTACGACTCATTGCAGCCGTTTCAGGTTTCTCGATTGTGATGCATCTTTTTCTCGGTTTTTGAGTTGAAATGGTTGAGCTTCTTTCGCAGGGGGAAGATCCTTGAGGTCTTGCGGAATTGGCCTGAGAAGAACATTCAAGTCATTGTAGTTACTGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGCCAGGTTAATTCCCTTCGCAACTCTTAAGGAAACTTGTTTAAAAGTATTTCAAATAATGATTGTCATCTGAAGCTTGAGATTGGGCAATGAACTCTTTCTTCTTGTCTGTTATTGAACCTAATGATCCTAACACACAGCAAAATCCATGTGAATATTTCAGGGGATGGGCATACCCGTTGGAAAACTCTCTTTATATACTGCACTTGGGGGTGTTCGTCCTTCTGCAGTGAGTACCTACATGATCTCTTCCTATTCCTATATAATCTCTATTTATCTTGTTATAAAAATAAAAAAAAAATAACAATAATTTCTACTTACATCAACCTATGCTGATTTCCAAAATTAACCTTGCTGCTTCATTTGTTTTTCTTGTTGGTTCACTGTGAAATTTTTCGTTTTTTCAGTGCTTGCCCGTGACAATTGATGTTGGTACAAACAATGAGGAGTTGTTGAATGATGAGTTTTACATTGGGCTCAGGCAGAAGAGAGCTACCGGGCAGGTTTGTGACACCCCTTCTCTCTCTCTCTCTCTCTCTAGCCGATGTATCATTTGTAGTTCAATGTGACAAGTCATATTGCATTCGAGCTTAAGTGGAATGATTTTTCTCAATGAAGGAATATGCTGAACTTATAGATGAATTCATGACTGCGGTCAAACAGTGTTATGGGGAGAAGCTCCTCATTCAGGTATTTTGCTATAACTATGCATACTTATTCTGTGTAGTTAAGCAAATGCATTCCTTTTTTTTTTTTTACTCATTCTGATATTTGTCTTATTCAATTTCCACATTTTGAAGTTTGAAGACTTTGCAAACCATAATGCATTTGACTTGCTAGCAAAATATGGCGCAACTCATCTGGTCTTTAATGATGACATTCAGGTTAGAACCCTGGACTTTCCGTTTTACTATTTGAATAATTTCAATCAACTTAACTATAATTGTCATCGTTGAATCTCAGGGGACAGCATCCGTGGTACTTGCTGGGCTCATTTCAGCTTTAAAGTTTGTTGGCGGATCCTTAGCTGACCATAGGTTTTTGTTCCTTGGTGCTGGAGAAGTAAGTCCACATTGAATCGTGTCTAACATCATAAAAAAGCTCCTTCTAATGTCAATTTACTATATTAACGTTTGGTGTTGCATTGGTTGTTTCAGGCTGGTACCGGAATTGCGGAGCTCATAGCCCTTGAGATATCAAAACAGGTTAAAATGATAGTCATTTTTAGACTATAGATTTTCATCCTACTTTGTTCTGAATGCATTTATTTAATCTTCATCTTAATTAACAAGAGCTAGACCTTTGTTTTCTGTAGACCAATGCTCCGTTGGAAGAGACACGCAAAAAAGTCTGGCTCGTGGATTCCAAGGTAACTTATTCATCAAGCTTGAGGAGTTGTTCATCATTCATAAAGTTTTTTGATAGTTTATATCTAATTCCGTTTTTATTTACTTCCTCAGGGGCTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGAAACTCATTGATGCCGTTAAGGTGTCAAATCTTTCTATGTACCTCTACAATTGTACATTCATAATGTGAACCAAGTTTTTAAGTTCTTCTATTTACTGTTTCACATCAGGAAATCAAACCAACCGTCTTGATTGGAACATCAGGCGTTGGAAGAACATTCACTAAAGAAGTCGTGGAGACTATGGCCTCAATCAATGAGGTATTTAATCCGTCTGACAGATTATTTCTTTATTGTTCAGTTTTAGAAGAATAGATGCTCATCATGATTTGGGTAACTTGTTGCAGAAACCTATCATTCTCTCTCTTTCAAATCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTACAAATGGAGTCAGGTAATACAACCCCCCACAACCCCACAAGATTATGGAAAGGGTGGAAAATGCATATTGATTTTGTTTTGTATGACATTGTTCGGTTGTTGCATTCAGGGTCGTGCCATTTTCGCAAGCGGGAGCCCATTTGACCCAGTTAAATACAAGGGGAAAGTGTTTGTGCCCGGCCAGGTCCTCACATTTCAATTCAATATAGTCTATAAGCTAGGCACATATCATGATTTGGGTGTTAAAAGTACTTGTTGGTGATTTATTAACAATTCAGGCAAACAATGCGTACATCTTTCCTGGATTGGGTTTGGGGTTAATTATGTCAGGCACAATCCGCGTTCACGATGACATGCTTCTAGCAGCCTGTAAGTACTCAAAAGCCTATTATGATTAAAGTTCGTATATTAGAGACAGTATACTTACTTTTCAAAGCTTAGATGGGCTATAGTCCATATCTATGTTTAAGTTATCATTTTTATTGAGTCGGGTTTTGAACCTTCATATCATATTGATGTACTTGTTTATTACAATCAGCGGAAGCTTTGGCATCACAAGTGTCCCAAGACGATTTCGACAGAGGACTCATCTATCCGCCATTTACCAACATCAGAAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTGAGCCTTCAACTTCATTTCCCTTTCCCAAACCTTTCAAAGAGTTTCAATTTGCTAATCATTCGCCCATTTCAAATTCTGTCTTATAGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTACCGGTAG

mRNA sequence

ATGGGTGTTTCTGGAGCTTGCTCCACTCCCTCCGGCACCGGGAGACGGCAAGCTGTGGTTCTGAAGGTGGTGTCGATGGATTCGAATGGTCGAGCCGGCGAGCGAAGCGGCAGTGTTGTGGTGGAGAACAACACTGTGAAAGAAATTGCCAAACCTCCCTCGCCGGTGGTTGAAGTGGAACCCAAATCCACCGCCGGCGCCTGGGCTCAGGATGCGTATGTTGAAGATTCCGCCGCCGCCACGGAGGAACAAATCATGACACCTTGGAGCGTCTCTGTTGCAAGTGGATATAATTTATTGCGAGACCCACGGCACAACAAAGGGCTTGCGTTTACTGAAAAAGAAAGAGACGCTCACTACTTGCGCGGTCTTCTACCCCCGACAATTGTTGCTCAAAATCTTCAGGTAAAGAAGATGTTGCACAATATCCGTCAATATCAAGTACCATTGCAGAAGTACATGGCAATGATGGATCTTCAGGAGAGGAATGAGAAGTTGTTTTACAAGCTCCTTATAGATCATGTAGAAGAATTGCTCCCAGTTGTCTATACACCCACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCAACCACAGGGTCTATATATCAGTTTGAGAGAAAAGGGGAAGATCCTTGAGGTCTTGCGGAATTGGCCTGAGAAGAACATTCAAGTCATTGTAGTTACTGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGCCAGGGGATGGGCATACCCGTTGGAAAACTCTCTTTATATACTGCACTTGGGGGTGTTCGTCCTTCTGCATGCTTGCCCGTGACAATTGATGTTGGTACAAACAATGAGGAGTTGTTGAATGATGAGTTTTACATTGGGCTCAGGCAGAAGAGAGCTACCGGGCAGGAATATGCTGAACTTATAGATGAATTCATGACTGCGGTCAAACAGTGTTATGGGGAGAAGCTCCTCATTCAGTTTGAAGACTTTGCAAACCATAATGCATTTGACTTGCTAGCAAAATATGGCGCAACTCATCTGGTCTTTAATGATGACATTCAGGCTGGTACCGGAATTGCGGAGCTCATAGCCCTTGAGATATCAAAACAGACCAATGCTCCGTTGGAAGAGACACGCAAAAAAGTCTGGCTCGTGGATTCCAAGGGGCTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGAAACTCATTGATGCCGTTAAGGAAATCAAACCAACCGTCTTGATTGGAACATCAGGCGTTGGAAGAACATTCACTAAAGAAGTCGTGGAGACTATGGCCTCAATCAATGAGAAACCTATCATTCTCTCTCTTTCAAATCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTACAAATGGAGTCAGGGTCGTGCCATTTTCGCAAGCGGGAGCCCATTTGACCCAGTTAAATACAAGGGGAAAGTGTTTGTGCCCGGCCAGGCAAACAATGCGTACATCTTTCCTGGATTGGGTTTGGGGTTAATTATGTCAGGCACAATCCGCGTTCACGATGACATGCTTCTAGCAGCCTCGGAAGCTTTGGCATCACAAGTGTCCCAAGACGATTTCGACAGAGGACTCATCTATCCGCCATTTACCAACATCAGAAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTACCGGTAG

Coding sequence (CDS)

ATGGGTGTTTCTGGAGCTTGCTCCACTCCCTCCGGCACCGGGAGACGGCAAGCTGTGGTTCTGAAGGTGGTGTCGATGGATTCGAATGGTCGAGCCGGCGAGCGAAGCGGCAGTGTTGTGGTGGAGAACAACACTGTGAAAGAAATTGCCAAACCTCCCTCGCCGGTGGTTGAAGTGGAACCCAAATCCACCGCCGGCGCCTGGGCTCAGGATGCGTATGTTGAAGATTCCGCCGCCGCCACGGAGGAACAAATCATGACACCTTGGAGCGTCTCTGTTGCAAGTGGATATAATTTATTGCGAGACCCACGGCACAACAAAGGGCTTGCGTTTACTGAAAAAGAAAGAGACGCTCACTACTTGCGCGGTCTTCTACCCCCGACAATTGTTGCTCAAAATCTTCAGGTAAAGAAGATGTTGCACAATATCCGTCAATATCAAGTACCATTGCAGAAGTACATGGCAATGATGGATCTTCAGGAGAGGAATGAGAAGTTGTTTTACAAGCTCCTTATAGATCATGTAGAAGAATTGCTCCCAGTTGTCTATACACCCACCGTGGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCAGGCAACCACAGGGTCTATATATCAGTTTGAGAGAAAAGGGGAAGATCCTTGAGGTCTTGCGGAATTGGCCTGAGAAGAACATTCAAGTCATTGTAGTTACTGATGGGGAGAGAATTTTGGGGCTAGGGGATCTTGGATGCCAGGGGATGGGCATACCCGTTGGAAAACTCTCTTTATATACTGCACTTGGGGGTGTTCGTCCTTCTGCATGCTTGCCCGTGACAATTGATGTTGGTACAAACAATGAGGAGTTGTTGAATGATGAGTTTTACATTGGGCTCAGGCAGAAGAGAGCTACCGGGCAGGAATATGCTGAACTTATAGATGAATTCATGACTGCGGTCAAACAGTGTTATGGGGAGAAGCTCCTCATTCAGTTTGAAGACTTTGCAAACCATAATGCATTTGACTTGCTAGCAAAATATGGCGCAACTCATCTGGTCTTTAATGATGACATTCAGGCTGGTACCGGAATTGCGGAGCTCATAGCCCTTGAGATATCAAAACAGACCAATGCTCCGTTGGAAGAGACACGCAAAAAAGTCTGGCTCGTGGATTCCAAGGGGCTGATTGTAAGTTCTAGGAAGGATACTCTCCAACATTTCAAGAAACCTTGGGCTCATGAACACAAACCTATCAAGAAACTCATTGATGCCGTTAAGGAAATCAAACCAACCGTCTTGATTGGAACATCAGGCGTTGGAAGAACATTCACTAAAGAAGTCGTGGAGACTATGGCCTCAATCAATGAGAAACCTATCATTCTCTCTCTTTCAAATCCTACTTCCCAATCTGAATGTACTGCTGAAGAAGCTTACAAATGGAGTCAGGGTCGTGCCATTTTCGCAAGCGGGAGCCCATTTGACCCAGTTAAATACAAGGGGAAAGTGTTTGTGCCCGGCCAGGCAAACAATGCGTACATCTTTCCTGGATTGGGTTTGGGGTTAATTATGTCAGGCACAATCCGCGTTCACGATGACATGCTTCTAGCAGCCTCGGAAGCTTTGGCATCACAAGTGTCCCAAGACGATTTCGACAGAGGACTCATCTATCCGCCATTTACCAACATCAGAAAGATTTCAGCACATATTGCTGCCAGTGTGGCTGCAAAAGCTTATGAACTTGGTTTGGCCACTCGCCTCCCTCAACCTAAAGATCTGGTGAAATATGCTGAGAGCTGTATGTACAGTCCTAGCTATCAAAATTACCGGTAG

Protein sequence

MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Homology
BLAST of HG10002292 vs. NCBI nr
Match: XP_038900124.1 (NADP-dependent malic enzyme-like [Benincasa hispida])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 590/635 (92.91%), Postives = 593/635 (93.39%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTGRRQ V LKVVSMDSNGRAGERSGSVVVENNTVKEIAKP SPVVEVE
Sbjct: 13  MGVSGACSTPSGTGRRQPVALKVVSMDSNGRAGERSGSVVVENNTVKEIAKPLSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
            KST G WAQD YVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  TKSTTGTWAQDVYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPT+VAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTVVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ        
Sbjct: 313 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEE RKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEEMRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHE+KPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHENKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF
Sbjct: 493 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQDDFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 647

BLAST of HG10002292 vs. NCBI nr
Match: XP_008447551.1 (PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >XP_016900422.1 PREDICTED: NADP-dependent malic enzyme [Cucumis melo])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/635 (91.81%), Postives = 596/635 (93.86%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 493 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 647

BLAST of HG10002292 vs. NCBI nr
Match: TYK08505.1 (NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/635 (91.81%), Postives = 596/635 (93.86%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 1   MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 60

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 61  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 240

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 301 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 360

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 361 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 481 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 540

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 541 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 600

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 635

BLAST of HG10002292 vs. NCBI nr
Match: XP_004150784.1 (NADP-dependent malic enzyme [Cucumis sativus] >KGN58034.1 hypothetical protein Csa_009953 [Cucumis sativus])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 579/635 (91.18%), Postives = 592/635 (93.23%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSG CSTPSGTG R+ + LKVVSMDSNGRAG+RSGSV+VENNTVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGPCSTPSGTGNRRPMALKVVSMDSNGRAGDRSGSVLVENNTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAGAW QDAYVEDSA A  EQIMTPWS+SVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGAWPQDAYVEDSAPAMNEQIMTPWSLSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEH+PIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHEHRPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 493 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 647

BLAST of HG10002292 vs. NCBI nr
Match: KAA0050846.1 (NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 583/661 (88.20%), Postives = 596/661 (90.17%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQVRTLDFLF 372

Query: 361 ---------------------------------------------------AGTGIAELI 420
                                                              AGTGIAELI
Sbjct: 373 YYLCNLNNSTIIIIVEFQGTASVVLAGLISALKVVGGSLADHRFLFLGAGEAGTGIAELI 432

Query: 421 ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI 480
           ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI
Sbjct: 433 ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI 492

Query: 481 KPTVLIGTSGVGRTFTKEVVETMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFA 540
           KPTVLIGTSGVGRTFTKEVVETMASINE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFA
Sbjct: 493 KPTVLIGTSGVGRTFTKEVVETMASINEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFA 552

Query: 541 SGSPFDPVKYKGKVFVPGQANNAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQD 600
           SGSPFDPVK+KGK+FVPGQANNAYIFPG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+
Sbjct: 553 SGSPFDPVKFKGKMFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALASQVSQE 612

Query: 601 DFDRGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY 603
           DFD+GLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY
Sbjct: 613 DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY 672

BLAST of HG10002292 vs. ExPASy Swiss-Prot
Match: Q9CA83 (NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NADP-ME4 PE=1 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 2.7e-263
Identity = 460/632 (72.78%), Postives = 527/632 (83.39%), Query Frame = 0

Query: 7   CSTPSGTGRRQAVVLKVVSM---DSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVEPKS 66
           CST     + + ++    S+    S G   + +GSV++E  T    +   +   ++ PKS
Sbjct: 18  CSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIE-TTATSSSSLETSAADIVPKS 77

Query: 67  TAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYLRG 126
           T     QD Y ED  AATE+  +TPWS+SVASGY LLRDP HNKGLAF+ +ERDAHYLRG
Sbjct: 78  TVSGGVQDVYGED--AATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRG 137

Query: 127 LLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPVVY 186
           LLPPT+++Q+LQVKK++H +RQYQVPLQKYMAMMDLQE NE+LFYKLLIDHVEELLPV+Y
Sbjct: 138 LLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIY 197

Query: 187 TPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLGDL 246
           TPTVGEACQKYGSIF +PQGL+ISL+EKGKI EVLRNWPEKNIQVIVVTDGERILGLGDL
Sbjct: 198 TPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDL 257

Query: 247 GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYA 306
           GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNE+LLNDEFYIGLRQ+RATG+EY+
Sbjct: 258 GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYS 317

Query: 307 ELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ----------- 366
           EL+ EFMTAVKQ YGEK++IQFEDFANHNAFDLLAKYG THLVFNDDIQ           
Sbjct: 318 ELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLI 377

Query: 367 ----------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIV 426
                                 AGTGIAELIALEISK+++ PLEE RK +WLVDSKGLIV
Sbjct: 378 AALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIV 437

Query: 427 SSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASINEK 486
           SSRK+++QHFKKPWAH+H+PI++L+DAVK IKPTVLIGTSGVG+TFT++VVETMA +NEK
Sbjct: 438 SSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEK 497

Query: 487 PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFPGL 546
           PIILSLSNPTSQSECTAEEAY WSQGRAIFASGSPF PV+Y+GK FVPGQANNAYIFPG 
Sbjct: 498 PIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGF 557

Query: 547 GLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAAKA 603
           GLGLIMSGTIRVHDDMLLAASEALA ++ ++ +++G+IYPPF NIRKISA IAA VAAKA
Sbjct: 558 GLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKA 617

BLAST of HG10002292 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 7.9e-263
Identity = 449/592 (75.84%), Postives = 516/592 (87.16%), Query Frame = 0

Query: 44  NTVKEIAKPPSPVVEVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDP 103
           +T+K+I +  + V++++PK+T G   +D Y ED   ATE+Q++TPW+VSVASGY+LLRDP
Sbjct: 3   STLKDI-RDGASVLDLDPKATVGGGVEDLYGED--FATEDQLVTPWTVSVASGYSLLRDP 62

Query: 104 RHNKGLAFTEKERDAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERN 163
           RHNKGLAF +KERDAHYL GLLPP +  Q LQ +K++++IRQYQVPLQKYMAMMDLQERN
Sbjct: 63  RHNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERN 122

Query: 164 EKLFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPE 223
           E+LFYKLLID+VEELLPVVYTPTVGEACQKYGSIFR+PQGLYISL+EKGKILEVL+NWPE
Sbjct: 123 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPE 182

Query: 224 KNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEEL 283
           + IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLP+TIDVGTNNE+L
Sbjct: 183 RRIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKL 242

Query: 284 LNDEFYIGLRQKRATGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGAT 343
           L +EFYIGL+Q+RATG+EY+E + EFM+ VKQ YGEK+LIQFEDFANHNAFDLLAKYG T
Sbjct: 243 LANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 302

Query: 344 HLVFNDDIQ---------------------------------AGTGIAELIALEISKQTN 403
           HL FNDDIQ                                 AGTGIAELIALE+SKQT 
Sbjct: 303 HLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTK 362

Query: 404 APLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTS 463
            P+EETRKK+WLVDSKGLIV SRKD+LQ FKKPWAHEH+P+K L+DAVK IKPTVLIG+S
Sbjct: 363 CPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSS 422

Query: 464 GVGRTFTKEVVETMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK 523
           GVG+ FTKEV+E MAS NEKP+IL+LSNPTSQSECTAEEAY W+QGRAIFASGSPFDPV+
Sbjct: 423 GVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVE 482

Query: 524 YKGKVFVPGQANNAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYP 583
           Y GK FVPGQANNAYIFPGLG+GL++SG IRVHD+MLLAASEALA QV+Q++FD+GLIYP
Sbjct: 483 YNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYP 542

Query: 584 PFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           PF+NIRKISAHIAA+VAAKAYELGLATRLPQP++LVKYAESCMYSP Y++YR
Sbjct: 543 PFSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSYR 591

BLAST of HG10002292 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 3.0e-262
Identity = 462/638 (72.41%), Postives = 528/638 (82.76%), Query Frame = 0

Query: 3   VSGACSTPSGTGRRQAVVLKVVSM-DSNGRAGERSGSVVVENNTVKEIAKPPSPVV---- 62
           +SG   T S + R       VV+M +SNGR  ERS  V V+   VK++  P +  V    
Sbjct: 14  ISGVSWTQSQSVRLSVRRPMVVAMVNSNGRP-ERSVGVSVD-GAVKDVNAPVAVEVADSE 73

Query: 63  EVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERD 122
             +P +  G   +D Y EDS  ATE+  +TPWSVSVASGY+LLRDP HNKGLAFTEKERD
Sbjct: 74  SKKPTAVVGGGVEDVYGEDS--ATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERD 133

Query: 123 AHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEE 182
           AHYLRGLLPP +V  +LQVKKM+HNIRQY+VPLQ+Y AMMDLQERNE+LFYKLLI+++EE
Sbjct: 134 AHYLRGLLPPVVVNHDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEE 193

Query: 183 LLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERI 242
           LLP+VYTPTVGEACQKYG+IF+ PQGLYISL++KGK+LE+L+NWP+K IQVIVVTDGERI
Sbjct: 194 LLPIVYTPTVGEACQKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERI 253

Query: 243 LGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRA 302
           LGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTNNE++LNDEFYIGLRQ+RA
Sbjct: 254 LGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRA 313

Query: 303 TGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ----- 362
           +G+EYAEL++EFM+AVKQ YGEK+LIQFEDFANHNAFDLL KY  THLVFNDDIQ     
Sbjct: 314 SGKEYAELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASV 373

Query: 363 ----------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVD 422
                                       AGTGIAELIALEISKQTNAPLEETRKK+WLVD
Sbjct: 374 VLAGLISALKLVGGSLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVD 433

Query: 423 SKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETM 482
           SKGLIV SR D+LQHFKKPWAH+H+P+ K +DAVK IKPTVLIG+SG G+TFTKEVVE M
Sbjct: 434 SKGLIVRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAM 493

Query: 483 ASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNA 542
           +S NEKPIIL+LSNPTSQSECTAE+AY WS+GR IFASGSPF PV+Y GKV+V GQ+NNA
Sbjct: 494 SSFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNA 553

Query: 543 YIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAA 602
           YIFPG GLGLI+SG IRVHD+MLLAASEALA QV+Q+ FD GLIYPPFTNIRKISAHIAA
Sbjct: 554 YIFPGFGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAA 613

BLAST of HG10002292 vs. ExPASy Swiss-Prot
Match: P34105 (NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3)

HSP 1 Score: 894.4 bits (2310), Expect = 6.9e-259
Identity = 439/592 (74.16%), Postives = 515/592 (86.99%), Query Frame = 0

Query: 44  NTVKEIAKPPSPVVEVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDP 103
           +T+KE+ +  + V++++PKST G   +D Y ED   ATE+Q++TPW++SVASGY LLRDP
Sbjct: 3   STLKEM-RDGASVLDMDPKSTVGGGVEDVYGED--RATEDQLVTPWTISVASGYTLLRDP 62

Query: 104 RHNKGLAFTEKERDAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERN 163
            HNKGLAFTEKERDAHYLRGLLPPT ++Q LQ KK+++ IRQYQ+PLQKY AMM+L+ERN
Sbjct: 63  HHNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERN 122

Query: 164 EKLFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPE 223
           E+LFYKLLID+VEELLPVVYTPTVGEACQKYGSIF++PQGLYISL+EKGK+L+VL+NWP+
Sbjct: 123 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQ 182

Query: 224 KNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEEL 283
           K+IQVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGGVRPSACLPVTIDVGTNNE+L
Sbjct: 183 KSIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQL 242

Query: 284 LNDEFYIGLRQKRATGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGAT 343
           L DEFYIGLRQ+RATGQEY+EL+ EFMTAVKQ YGEK+LIQFEDFANHNAFDLLAKYG T
Sbjct: 243 LKDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 302

Query: 344 HLVFNDDIQ---------------------------------AGTGIAELIALEISKQTN 403
           HLVFNDDIQ                                 AGTGIAELIALE+S+++ 
Sbjct: 303 HLVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSK 362

Query: 404 APLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTS 463
            PLEETRKK+WL DSKGLIVSSRK++LQHFKKPWAHEH+P+K L++ VK IKP VLIGTS
Sbjct: 363 TPLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTS 422

Query: 464 GVGRTFTKEVVETMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK 523
           GVG+TFTKEV+E MAS NEKP+IL+LSNPTSQSECTA+EAY W++G+AIFASGSPFDPV+
Sbjct: 423 GVGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVE 482

Query: 524 YKGKVFVPGQANNAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYP 583
           Y+GKVFVPGQ+NNAYIFPGLGLGL++SG IRVHDDMLLAA+EALA Q+ ++   +GLIYP
Sbjct: 483 YEGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYP 542

Query: 584 PFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           P +NIRKIS  IAA+VAAKAYELGLATRLP+P++LVK+AESCMYSP+Y+ YR
Sbjct: 543 PLSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAYRYYR 591

BLAST of HG10002292 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 6.5e-257
Identity = 440/579 (75.99%), Postives = 504/579 (87.05%), Query Frame = 0

Query: 57  VEVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKER 116
           V V+   + G   +D Y EDS  ATE+ ++TPW+ SVASG +LLRDPR+NKGLAFTE ER
Sbjct: 13  VSVKKDYSNGGGVRDLYGEDS--ATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTEGER 72

Query: 117 DAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVE 176
           DAHYLRGLLPP++  Q LQ K+++HN+RQY+VPL +YMA+MDLQERNE+LFYKLLID+V 
Sbjct: 73  DAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLFYKLLIDNVA 132

Query: 177 ELLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGER 236
           ELLPVVYTPTVGEACQKYGSIFR+PQGLYISL+EKGKILEVL+NWPEK+IQVIVVTDGER
Sbjct: 133 ELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGER 192

Query: 237 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKR 296
           ILGLGDLGCQGMGIPVGKLSLYTALGGVRPS+CLPVTIDVGTNNE+LLNDEFYIGLRQ+R
Sbjct: 193 ILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRR 252

Query: 297 ATGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ---- 356
           ATGQEYA  +DEFM AVKQ YGEK+L+QFEDFANHNAFDLL KY ++HLVFNDDIQ    
Sbjct: 253 ATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVFNDDIQGTAS 312

Query: 357 -----------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLV 416
                                        AGTGIAELIA+E+SKQT AP+EETRKK+WLV
Sbjct: 313 VVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVEETRKKIWLV 372

Query: 417 DSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVET 476
           DSKGLIVSSR ++LQ FKKPWAHEH+P+K L++AVK IKPTVLIG+SG G+TFTKEVVET
Sbjct: 373 DSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVET 432

Query: 477 MASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANN 536
           MAS+NEKP+IL+LSNPTSQSECTAEEAY WS+GRAIFASGSPFDPV+Y+GK+FVPGQANN
Sbjct: 433 MASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQANN 492

Query: 537 AYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIA 596
           AYIFPG GLGLIMSG IRV D+MLLAASEALA+QVS++++D+GLIYPPFTNIRKISA+IA
Sbjct: 493 AYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTNIRKISANIA 552

Query: 597 ASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           A VAAKAY+LGLA+ L +PKDLVKYAESCMYSP Y++YR
Sbjct: 553 AKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSYR 589

BLAST of HG10002292 vs. ExPASy TrEMBL
Match: A0A1S4DWR5 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/635 (91.81%), Postives = 596/635 (93.86%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 493 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 647

BLAST of HG10002292 vs. ExPASy TrEMBL
Match: A0A5D3CAD9 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 PE=3 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/635 (91.81%), Postives = 596/635 (93.86%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 1   MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 60

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 61  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 240

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 301 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 360

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 361 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 481 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 540

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 541 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 600

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 635

BLAST of HG10002292 vs. ExPASy TrEMBL
Match: A0A0A0LAT4 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G444620 PE=3 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 579/635 (91.18%), Postives = 592/635 (93.23%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSG CSTPSGTG R+ + LKVVSMDSNGRAG+RSGSV+VENNTVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGPCSTPSGTGNRRPMALKVVSMDSNGRAGDRSGSVLVENNTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAGAW QDAYVEDSA A  EQIMTPWS+SVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGAWPQDAYVEDSAPAMNEQIMTPWSLSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG
Sbjct: 373 GLISALKVVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIVSSRKDTLQHFKKPWAHEH+PIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVSSRKDTLQHFKKPWAHEHRPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVK+KGK+FVPGQANNAYIF
Sbjct: 493 NEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKFKGKMFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+DFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVSQEDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 647

BLAST of HG10002292 vs. ExPASy TrEMBL
Match: A0A5A7U9H4 (NADP-dependent malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold404G001150 PE=3 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 583/661 (88.20%), Postives = 596/661 (90.17%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSGTG R+ + LKVVSMDSNGRAGERSGSV+VEN+TVKEIAKPPSPVVEVE
Sbjct: 13  MGVSGACSTPSGTGNRRPMALKVVSMDSNGRAGERSGSVLVENSTVKEIAKPPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTAG+WAQDAYVEDSAAA +EQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTAGSWAQDAYVEDSAAAMDEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKG+ILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGRILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAEL+DEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYG THLVFNDDIQ        
Sbjct: 313 EYAELMDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGPTHLVFNDDIQVRTLDFLF 372

Query: 361 ---------------------------------------------------AGTGIAELI 420
                                                              AGTGIAELI
Sbjct: 373 YYLCNLNNSTIIIIVEFQGTASVVLAGLISALKVVGGSLADHRFLFLGAGEAGTGIAELI 432

Query: 421 ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI 480
           ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI
Sbjct: 433 ALEISKQTNAPLEETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEI 492

Query: 481 KPTVLIGTSGVGRTFTKEVVETMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFA 540
           KPTVLIGTSGVGRTFTKEVVETMASINE+PIILSLSNPTSQSECTAEEAYKWSQGRAIFA
Sbjct: 493 KPTVLIGTSGVGRTFTKEVVETMASINEQPIILSLSNPTSQSECTAEEAYKWSQGRAIFA 552

Query: 541 SGSPFDPVKYKGKVFVPGQANNAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQD 600
           SGSPFDPVK+KGK+FVPGQANNAYIFPG GLGLIMSGTIRVHDDMLLAASEALASQVSQ+
Sbjct: 553 SGSPFDPVKFKGKMFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALASQVSQE 612

Query: 601 DFDRGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY 603
           DFD+GLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY
Sbjct: 613 DFDKGLIYPPFTNIRKISAHIAASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNY 672

BLAST of HG10002292 vs. ExPASy TrEMBL
Match: A0A6J1DJF8 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 579/635 (91.18%), Postives = 591/635 (93.07%), Query Frame = 0

Query: 1   MGVSGACSTPSGTGRRQAVVLKVVSMDSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVE 60
           MGVSGACSTPSG GRR+   LKV+SMDSNGRAGER GSVVVENNTVKEIAK PSPVVEVE
Sbjct: 13  MGVSGACSTPSGNGRRRPAALKVLSMDSNGRAGERGGSVVVENNTVKEIAKAPSPVVEVE 72

Query: 61  PKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 120
           PKSTA  WAQDAYVED AAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY
Sbjct: 73  PKSTARGWAQDAYVED-AAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHY 132

Query: 121 LRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 180
           LRGLLPPT+VAQ+LQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP
Sbjct: 133 LRGLLPPTVVAQDLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLP 192

Query: 181 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 240
           VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL
Sbjct: 193 VVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGL 252

Query: 241 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 300
           GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ
Sbjct: 253 GDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQ 312

Query: 301 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ-------- 360
           EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ        
Sbjct: 313 EYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQGTASVVLA 372

Query: 361 -------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKG 420
                                    AGTGIAELIALEISKQTNAPLEETRKK+WLVDSKG
Sbjct: 373 GLVAALKFVGGSLADHRFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKG 432

Query: 421 LIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASI 480
           LIV+SRK+TLQHFKKPWAHEHKPIKKL+DAVKEI+PT+LIGTSGVGRTFTKEVVETMASI
Sbjct: 433 LIVNSRKETLQHFKKPWAHEHKPIKKLVDAVKEIRPTILIGTSGVGRTFTKEVVETMASI 492

Query: 481 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 540
           NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF
Sbjct: 493 NEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIF 552

Query: 541 PGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVA 600
           PG GLGLIMSGTIRVHDDMLLAASEALASQV+QDDFD+GLIYPPFTNIRKISAHIAASVA
Sbjct: 553 PGFGLGLIMSGTIRVHDDMLLAASEALASQVTQDDFDKGLIYPPFTNIRKISAHIAASVA 612

Query: 601 AKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AKAYELGLATRLPQPKDL KYAESCMYSPSYQNYR
Sbjct: 613 AKAYELGLATRLPQPKDLEKYAESCMYSPSYQNYR 646

BLAST of HG10002292 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-264
Identity = 460/632 (72.78%), Postives = 527/632 (83.39%), Query Frame = 0

Query: 7   CSTPSGTGRRQAVVLKVVSM---DSNGRAGERSGSVVVENNTVKEIAKPPSPVVEVEPKS 66
           CST     + + ++    S+    S G   + +GSV++E  T    +   +   ++ PKS
Sbjct: 18  CSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIE-TTATSSSSLETSAADIVPKS 77

Query: 67  TAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYLRG 126
           T     QD Y ED  AATE+  +TPWS+SVASGY LLRDP HNKGLAF+ +ERDAHYLRG
Sbjct: 78  TVSGGVQDVYGED--AATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRG 137

Query: 127 LLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPVVY 186
           LLPPT+++Q+LQVKK++H +RQYQVPLQKYMAMMDLQE NE+LFYKLLIDHVEELLPV+Y
Sbjct: 138 LLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIY 197

Query: 187 TPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLGDL 246
           TPTVGEACQKYGSIF +PQGL+ISL+EKGKI EVLRNWPEKNIQVIVVTDGERILGLGDL
Sbjct: 198 TPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDL 257

Query: 247 GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYA 306
           GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNE+LLNDEFYIGLRQ+RATG+EY+
Sbjct: 258 GCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYS 317

Query: 307 ELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ----------- 366
           EL+ EFMTAVKQ YGEK++IQFEDFANHNAFDLLAKYG THLVFNDDIQ           
Sbjct: 318 ELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLI 377

Query: 367 ----------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLIV 426
                                 AGTGIAELIALEISK+++ PLEE RK +WLVDSKGLIV
Sbjct: 378 AALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIV 437

Query: 427 SSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASINEK 486
           SSRK+++QHFKKPWAH+H+PI++L+DAVK IKPTVLIGTSGVG+TFT++VVETMA +NEK
Sbjct: 438 SSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEK 497

Query: 487 PIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFPGL 546
           PIILSLSNPTSQSECTAEEAY WSQGRAIFASGSPF PV+Y+GK FVPGQANNAYIFPG 
Sbjct: 498 PIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGF 557

Query: 547 GLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAAKA 603
           GLGLIMSGTIRVHDDMLLAASEALA ++ ++ +++G+IYPPF NIRKISA IAA VAAKA
Sbjct: 558 GLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKA 617

BLAST of HG10002292 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 864.8 bits (2233), Expect = 4.2e-251
Identity = 423/573 (73.82%), Postives = 493/573 (86.04%), Query Frame = 0

Query: 63  STAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYLR 122
           S  G    D Y EDS  AT +Q++TPW  SVASGY L+RDPR+NKGLAFT+KERDAHY+ 
Sbjct: 18  SGVGGGISDVYGEDS--ATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYIT 77

Query: 123 GLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPVV 182
           GLLPP +++Q++Q +K++HN+RQY VPLQ+YMA+MDLQERNE+LFYKLLID+VEELLPVV
Sbjct: 78  GLLPPVVLSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVV 137

Query: 183 YTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLGD 242
           YTPTVGEACQKYGSI+R+PQGLYISL+EKGKILEVL+NWP++ IQVIVVTDGERILGLGD
Sbjct: 138 YTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGD 197

Query: 243 LGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEY 302
           LGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTNNE+LLN+EFYIGL+QKRA G+EY
Sbjct: 198 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEY 257

Query: 303 AELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ---------- 362
           AE + EFM AVKQ YGEK+L+QFEDFANH+AF+LL+KY ++HLVFNDDIQ          
Sbjct: 258 AEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGL 317

Query: 363 -----------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGLI 422
                                  AGTGIAELIAL+ISK+T  P++ETRKK+WLVDSKGLI
Sbjct: 318 IAAQKVLGKSLADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLI 377

Query: 423 VSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASINE 482
           VS RK++LQHFK+PWAH+HKP+K+L+ AV  IKPTVLIGTSGVG+TFTKEVVE MA++NE
Sbjct: 378 VSERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNE 437

Query: 483 KPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFPG 542
           KP+IL+LSNPTSQ+ECTAEEAY W++GRAIFASGSPFDPV+Y GK F PGQANN YIFPG
Sbjct: 438 KPLILALSNPTSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPG 497

Query: 543 LGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAAK 602
           LGLGLIMSG IRV DDMLLAASEALASQV++++F  GLIYPPF NIRKISA+IAASV AK
Sbjct: 498 LGLGLIMSGAIRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAK 557

BLAST of HG10002292 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 862.1 bits (2226), Expect = 2.7e-250
Identity = 426/574 (74.22%), Postives = 490/574 (85.37%), Query Frame = 0

Query: 62  KSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKERDAHYL 121
           +S  G    D Y EDS  AT +Q++TPW  SVASGY L+RDPR+NKGLAFT+KERDAHYL
Sbjct: 17  RSGVGGGISDVYGEDS--ATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYL 76

Query: 122 RGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHVEELLPV 181
            GLLPP I++Q++Q +K++HN+RQY VPLQ+YMA+MDLQERNE+LFYKLLID+VEELLPV
Sbjct: 77  TGLLPPVILSQDVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPV 136

Query: 182 VYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLG 241
           VYTPTVGEACQKYGSIFR+PQGLYISL EKGKILEVL+NWP++ IQVIVVTDGERILGLG
Sbjct: 137 VYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLG 196

Query: 242 DLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQE 301
           DLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGL+Q+RATGQE
Sbjct: 197 DLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQE 256

Query: 302 YAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ--------- 361
           YAE + EFM AVKQ YGEK+L+QFEDFANHNAFDLL+KY  +HLVFNDDIQ         
Sbjct: 257 YAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAG 316

Query: 362 ------------------------AGTGIAELIALEISKQTNAPLEETRKKVWLVDSKGL 421
                                   AGTGIAELIAL+ISK+T AP+ ETRKK+WLVDSKGL
Sbjct: 317 LIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGL 376

Query: 422 IVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVETMASIN 481
           IVSSRK++LQHFK+PWAHEHKP+K LI AV  IKPTVLIGTSGVG+TFTKEVVE MA+ N
Sbjct: 377 IVSSRKESLQHFKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNN 436

Query: 482 EKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQANNAYIFP 541
           EKP+IL+LSNPTSQ+ECTAE+AY W++GRAIF SGSPFDPV Y GK ++PGQANN YIFP
Sbjct: 437 EKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFP 496

Query: 542 GLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAA 601
           GLGLGLIMSG IRV DDMLLAASEALA+QV+++ +  GLIYPPF+NIR+ISA+IAA VAA
Sbjct: 497 GLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAA 556

Query: 602 KAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           K Y+LGLA+ LP+ KDLVK+AES MYSP Y+NYR
Sbjct: 557 KTYDLGLASNLPRAKDLVKFAESSMYSPVYRNYR 588

BLAST of HG10002292 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 857.1 bits (2213), Expect = 8.7e-249
Identity = 426/580 (73.45%), Postives = 499/580 (86.03%), Query Frame = 0

Query: 56  VVEVEPKSTAGAWAQDAYVEDSAAATEEQIMTPWSVSVASGYNLLRDPRHNKGLAFTEKE 115
           V   + KS+      D Y EDS  AT E  +TPWS+SV+SGY+LLRDPR+NKGLAFTEKE
Sbjct: 4   VTNSDLKSSVDGGVVDVYGEDS--ATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKE 63

Query: 116 RDAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQVPLQKYMAMMDLQERNEKLFYKLLIDHV 175
           RD HYLRGLLPP ++ Q LQ K++L+NIRQYQ PLQKYMA+ +LQERNE+LFYKLLID+V
Sbjct: 64  RDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNV 123

Query: 176 EELLPVVYTPTVGEACQKYGSIFRQPQGLYISLREKGKILEVLRNWPEKNIQVIVVTDGE 235
           EELLP+VYTPTVGEACQK+GSIFR+PQGL+ISL++KGKIL+VL+NWPE+NIQVIVVTDGE
Sbjct: 124 EELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGE 183

Query: 236 RILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEELLNDEFYIGLRQK 295
           RILGLGDLGCQGMGIPVGKL+LY+ALGGVRPSACLPVTIDVGTNNE+LLNDEFYIGLRQK
Sbjct: 184 RILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQK 243

Query: 296 RATGQEYAELIDEFMTAVKQCYGEKLLIQFEDFANHNAFDLLAKYGATHLVFNDDIQ--- 355
           RATGQEY+EL++EFM+AVKQ YGEK+LIQFEDFANHNAF+LLAKY  THLVFNDDIQ   
Sbjct: 244 RATGQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTA 303

Query: 356 ------------------------------AGTGIAELIALEISKQTNAPLEETRKKVWL 415
                                         AGTGIAELIAL +SKQ NA +EE+RKK+WL
Sbjct: 304 SVVLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWL 363

Query: 416 VDSKGLIVSSRKDTLQHFKKPWAHEHKPIKKLIDAVKEIKPTVLIGTSGVGRTFTKEVVE 475
           VDSKGLIV+SRKD+LQ FKKPWAHEH+P+K L+ A+K IKPTVLIG+SGVGR+FTKEV+E
Sbjct: 364 VDSKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIE 423

Query: 476 TMASINEKPIILSLSNPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKYKGKVFVPGQAN 535
            M+SINE+P+I++LSNPT+QSECTAEEAY WS+GRAIFASGSPFDPV+Y+GKVFV  QAN
Sbjct: 424 AMSSINERPLIMALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQAN 483

Query: 536 NAYIFPGLGLGLIMSGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHI 595
           NAYIFPG GLGL++SG IRVHDDMLLAA+EALA QVS++++++G+IYP F++IRKISA I
Sbjct: 484 NAYIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQI 543

Query: 596 AASVAAKAYELGLATRLPQPKDLVKYAESCMYSPSYQNYR 603
           AA+VA KAYELGLA RLP+PKD+VK AES MYSP+Y+ YR
Sbjct: 544 AANVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYRLYR 581

BLAST of HG10002292 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-110
Identity = 226/572 (39.51%), Postives = 336/572 (58.74%), Query Frame = 0

Query: 96  GYNLLRDPRHNKGLAFTEKERDAHYLRGLLPPTIVAQNLQVKKMLHNIRQYQV------- 155
           G ++L DP  NKG AFT  ER+   LRGLLPP ++    Q+ + + ++++ +        
Sbjct: 46  GLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPS 105

Query: 156 ---PLQKYMAMMDLQERNEKLFYKLLIDHVEELLPVVYTPTVGEACQKYGSIFRQPQGLY 215
               L K+  +  L +RNE ++YK+LI+++EE  P+VYTPTVG  CQ Y  +FR+P+G+Y
Sbjct: 106 DPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMY 165

Query: 216 ISLREKGKILEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVR 275
            S  ++G+++ ++ NWP + + +IVVTDG RILGLGDLG  G+GI VGKL LY A  G+ 
Sbjct: 166 FSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGIN 225

Query: 276 PSACLPVTIDVGTNNEELLNDEFYIGLRQKRATGQEYAELIDEFMTAVKQCYGEKLLIQF 335
           P   LPV IDVGTNNE+L ND  Y+GL+Q+R    +Y ++IDEFM AV   +   +++QF
Sbjct: 226 PQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-PHVIVQF 285

Query: 336 EDFANHNAFDLLAKYGATHLVFNDDIQ-------------------------------AG 395
           EDF +  AF LL +Y  T+ +FNDD+Q                               AG
Sbjct: 286 EDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAG 345

Query: 396 TGIAELIALEISKQTNAPL--------EETRKKVWLVDSKGLIVSSRKDTLQHFKKPWAH 455
            G A +  L  +++T A +        +  + + W+VD++GLI   R + +    +P+A 
Sbjct: 346 AGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPEAQPFAR 405

Query: 456 EHKPIKK--------LIDAVKEIKPTVLIGTSGVGRTFTKEVVETM-ASINEKPIILSLS 515
           + K +++        L++ V+E+KP VL+G S VG  F+KEV+E M  S + +P I ++S
Sbjct: 406 KTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMS 465

Query: 516 NPTSQSECTAEEAYKWSQGRAIFASGSPFDPVKY-KGKVFVPGQANNAYIFPGLGLGLIM 575
           NPT  +ECT ++A+       IFASGSPF  V++  G V    Q NN Y+FPG+GLG ++
Sbjct: 466 NPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLL 525

Query: 576 SGTIRVHDDMLLAASEALASQVSQDDFDRGLIYPPFTNIRKISAHIAASVAAKAYELGLA 599
           SG   V D ML AASE LA+ +S+++   G+IYPP + IR I+  IAA+V  +A E  L 
Sbjct: 526 SGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLV 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900124.10.0e+0092.91NADP-dependent malic enzyme-like [Benincasa hispida][more]
XP_008447551.10.0e+0091.81PREDICTED: NADP-dependent malic enzyme [Cucumis melo] >XP_016900422.1 PREDICTED:... [more]
TYK08505.10.0e+0091.81NADP-dependent malic enzyme [Cucumis melo var. makuwa][more]
XP_004150784.10.0e+0091.18NADP-dependent malic enzyme [Cucumis sativus] >KGN58034.1 hypothetical protein C... [more]
KAA0050846.10.0e+0088.20NADP-dependent malic enzyme [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9CA832.7e-26372.78NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P516157.9e-26375.84NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
P364443.0e-26272.41NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ... [more]
P341056.9e-25974.16NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3[more]
P126286.5e-25775.99NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DWR50.0e+0091.81Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103489969 PE=3 SV=1[more]
A0A5D3CAD90.0e+0091.81Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00330 P... [more]
A0A0A0LAT40.0e+0091.18Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G444620 PE=3 SV=1[more]
A0A5A7U9H40.0e+0088.20NADP-dependent malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1DJF80.0e+0091.18Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111021036 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79750.11.9e-26472.78NADP-malic enzyme 4 [more]
AT5G25880.14.2e-25173.82NADP-malic enzyme 3 [more]
AT5G11670.12.7e-25074.22NADP-malic enzyme 2 [more]
AT2G19900.18.7e-24973.45NADP-malic enzyme 1 [more]
AT2G13560.11.5e-11039.51NAD-dependent malic enzyme 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 322..340
score: 71.84
coord: 166..190
score: 69.0
coord: 226..255
score: 82.75
coord: 262..284
score: 70.33
coord: 347..363
score: 40.59
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 352..602
e-value: 1.9E-96
score: 321.8
coord: 68..353
e-value: 3.3E-118
score: 393.7
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 160..341
e-value: 1.1E-102
score: 357.1
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 160..340
e-value: 2.2E-80
score: 268.7
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 336..571
e-value: 1.2E-81
score: 287.2
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 352..570
e-value: 8.1E-82
score: 274.4
NoneNo IPR availableGENE3D3.40.50.720coord: 344..598
e-value: 1.2E-76
score: 260.1
NoneNo IPR availablePIRSRPIRSR000106-3PIRSR000106-3coord: 98..352
e-value: 4.5E-114
score: 379.7
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 16..353
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 16..353
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 353..602
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 353..602
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 353..596
e-value: 4.47448E-134
score: 390.756
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 82..350
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 94..343
e-value: 9.1E-125
score: 416.9
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 352..601

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002292.1HG10002292.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity