HG10002246 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002246
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr11: 4844298 .. 4845275 (+)
RNA-Seq ExpressionHG10002246
SyntenyHG10002246
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCACCAGAATCATCACCGGCGCCGGAAACACTACCAAACTTCTGGGGCCACCAACCGGAGGAAGAATTCTACACCTCACAAGGCGTACGCAACACCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATCCCTCTGCACTCACCGGAGCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACGCCGGCTGGATGTTCCAAAAGATCTGCCTCTCCTACGCCTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGCCGCTCCGACGGAATCCGCTGCTACCTCGGTGACATGGACAAAATTGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACCGCCACCTCCCGGCGTTCCTCTTCGGCGAGTCCATGGGCGGCGCCGCCACCATGCTCATGTACTTCCAGTCCGATCCGGACACCTGGACCGGCCTAATCTTCTCAGCCCCCCTCTTCGTCATACCAGAAAACATGAAACCGAGCAAGCTCCACCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGATACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATCAAAGATCCGGAAAAGTTGAAGATCATAGCTGCGAACCCTAGAAGGTACACCGGACCGCCTCGGGTTGGGACGATGAGGGAATTGGTTAGAGTAACGCAGTACATTAGGGATAATTTTTCTAAGGTTACAGCGCCGTTTCTGACGGTGCACGGTACGGCGGACGTAGTGGCGTGTCCATCGTCGTCAGAATTACTATATGAGAAGGCGGCGAGTGTGGATAAGAGCTTGAAGCTTTACGATGGGATGTATCATTCATTGATTCAAGGAGAGCCTGATGAGAATATGGAGATTGTGCTGAGGGATATGAAGGAGTGGATCGATGAGAGGGTTTTGAGATATGGACCGAAGAAATAG

mRNA sequence

ATGCCACCAGAATCATCACCGGCGCCGGAAACACTACCAAACTTCTGGGGCCACCAACCGGAGGAAGAATTCTACACCTCACAAGGCGTACGCAACACCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATCCCTCTGCACTCACCGGAGCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACGCCGGCTGGATGTTCCAAAAGATCTGCCTCTCCTACGCCTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGCCGCTCCGACGGAATCCGCTGCTACCTCGGTGACATGGACAAAATTGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACCGCCACCTCCCGGCGTTCCTCTTCGGCGAGTCCATGGGCGGCGCCGCCACCATGCTCATGTACTTCCAGTCCGATCCGGACACCTGGACCGGCCTAATCTTCTCAGCCCCCCTCTTCGTCATACCAGAAAACATGAAACCGAGCAAGCTCCACCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGATACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATCAAAGATCCGGAAAAGTTGAAGATCATAGCTGCGAACCCTAGAAGGTACACCGGACCGCCTCGGGTTGGGACGATGAGGGAATTGGTTAGAGTAACGCAGTACATTAGGGATAATTTTTCTAAGGTTACAGCGCCGTTTCTGACGGTGCACGGTACGGCGGACGTAGTGGCGTGTCCATCGTCGTCAGAATTACTATATGAGAAGGCGGCGAGTGTGGATAAGAGCTTGAAGCTTTACGATGGGATGTATCATTCATTGATTCAAGGAGAGCCTGATGAGAATATGGAGATTGTGCTGAGGGATATGAAGGAGTGGATCGATGAGAGGGTTTTGAGATATGGACCGAAGAAATAG

Coding sequence (CDS)

ATGCCACCAGAATCATCACCGGCGCCGGAAACACTACCAAACTTCTGGGGCCACCAACCGGAGGAAGAATTCTACACCTCACAAGGCGTACGCAACACCAAATCCTTCTTCGAAACCCCCCATGGCAAGCTCTTCACACAGAGCTTCATCCCTCTGCACTCACCGGAGCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACGCCGGCTGGATGTTCCAAAAGATCTGCCTCTCCTACGCCTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGCCGCTCCGACGGAATCCGCTGCTACCTCGGTGACATGGACAAAATTGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGCAGCGAGCCCTACCGCCACCTCCCGGCGTTCCTCTTCGGCGAGTCCATGGGCGGCGCCGCCACCATGCTCATGTACTTCCAGTCCGATCCGGACACCTGGACCGGCCTAATCTTCTCAGCCCCCCTCTTCGTCATACCAGAAAACATGAAACCGAGCAAGCTCCACCTCTTCCTCTACGGCCTCCTCTTCGGCGTCGCCGATACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATCAAAGATCCGGAAAAGTTGAAGATCATAGCTGCGAACCCTAGAAGGTACACCGGACCGCCTCGGGTTGGGACGATGAGGGAATTGGTTAGAGTAACGCAGTACATTAGGGATAATTTTTCTAAGGTTACAGCGCCGTTTCTGACGGTGCACGGTACGGCGGACGTAGTGGCGTGTCCATCGTCGTCAGAATTACTATATGAGAAGGCGGCGAGTGTGGATAAGAGCTTGAAGCTTTACGATGGGATGTATCATTCATTGATTCAAGGAGAGCCTGATGAGAATATGGAGATTGTGCTGAGGGATATGAAGGAGTGGATCGATGAGAGGGTTTTGAGATATGGACCGAAGAAATAG

Protein sequence

MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLRDMKEWIDERVLRYGPKK
Homology
BLAST of HG10002246 vs. NCBI nr
Match: XP_038874501.1 (LOW QUALITY PROTEIN: caffeoylshikimate esterase [Benincasa hispida])

HSP 1 Score: 636.0 bits (1639), Expect = 1.8e-178
Identity = 303/325 (93.23%), Postives = 311/325 (95.69%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           MPPESSP  E  PNFWGHQPEEEFY SQGVRNTKSFFETPHGKLFTQSFIPL SPELKGT
Sbjct: 1   MPPESSPXSEPPPNFWGHQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPELKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWM+QKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDMDK+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMYQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIP+NMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPDNMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWA MPDNKMVGKAIKDPEKLKIIA+NPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWATMPDNKMVGKAIKDPEKLKIIASNPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNF +VTAPFLTVHGTAD V CPSSSELLYEKAASVDKSLKLYDGMYHSLIQGE D
Sbjct: 241 QYIRDNFYRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKSLKLYDGMYHSLIQGETD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           EN+EIVLRDM+EWIDER LRYGPKK
Sbjct: 301 ENVEIVLRDMREWIDERALRYGPKK 325

BLAST of HG10002246 vs. NCBI nr
Match: XP_008447481.1 (PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0038136.1 caffeoylshikimate esterase [Cucumis melo var. makuwa] >TYK20471.1 caffeoylshikimate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 627.1 bits (1616), Expect = 8.5e-176
Identity = 300/317 (94.64%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 9   PETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYG 68
           PE   NFWG QPEEEFY SQGVRNTKSFFETPHGKLFTQSFIPL SP+LKGTVYMTHGYG
Sbjct: 3   PEPPLNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTVYMTHGYG 62

Query: 69  SDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSE 128
           SD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDMDKIAAASLSFFLHTRRSE
Sbjct: 63  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 122

Query: 129 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLL 188
           PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL LFLYGLL
Sbjct: 123 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLL 182

Query: 189 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 248
           FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS
Sbjct: 183 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 242

Query: 249 KVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLR 308
           KVTAPFLTVHGTAD V CPSSS+LLYEKAASVDK+LKLYDGMYHSLIQGEPDEN+EIVLR
Sbjct: 243 KVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDENVEIVLR 302

Query: 309 DMKEWIDERVLRYGPKK 326
           DM+EWIDER LRYGPKK
Sbjct: 303 DMREWIDERALRYGPKK 319

BLAST of HG10002246 vs. NCBI nr
Match: XP_023525534.1 (caffeoylshikimate esterase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.2 bits (1611), Expect = 3.2e-175
Identity = 301/325 (92.62%), Postives = 311/325 (95.69%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           M PE  PAPETL N+WG QPEEEFY SQGV+N+KSFFETPHGKLFTQSFIPL SPE KGT
Sbjct: 1   MAPE-PPAPETLTNYWGDQPEEEFYASQGVQNSKSFFETPHGKLFTQSFIPLDSPEPKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDM+K+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKAASVDK+LKLY+GMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           ENMEIVLRDM+EWIDERV RYG KK
Sbjct: 301 ENMEIVLRDMREWIDERVQRYGSKK 324

BLAST of HG10002246 vs. NCBI nr
Match: XP_022949228.1 (caffeoylshikimate esterase [Cucurbita moschata])

HSP 1 Score: 625.2 bits (1611), Expect = 3.2e-175
Identity = 301/325 (92.62%), Postives = 311/325 (95.69%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           M PE + APETL N+WG QPEEEFY SQGVRN+KSFFETPHGKLFTQSFIPL SPE KGT
Sbjct: 1   MAPEPA-APETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDM+K+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKAASVDK+LKLY+GMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           ENMEIVLRDM+EWIDERV RYG KK
Sbjct: 301 ENMEIVLRDMREWIDERVQRYGSKK 324

BLAST of HG10002246 vs. NCBI nr
Match: KAG6606779.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036492.1 Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.8 bits (1610), Expect = 4.2e-175
Identity = 300/325 (92.31%), Postives = 311/325 (95.69%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           M PE  PAPETL N+WG QPEEEFY SQGVRN+KSFFETPHGKLFTQSFIPL SPE KGT
Sbjct: 1   MAPE-PPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDM+K+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKAASVDK++KLY+GMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTMKLYEGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           ENMEIVLRDM+EWIDERV RYG K+
Sbjct: 301 ENMEIVLRDMREWIDERVQRYGSKR 324

BLAST of HG10002246 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 8.9e-152
Identity = 249/321 (77.57%), Postives = 288/321 (89.72%), Query Frame = 0

Query: 5   SSPA-PETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYM 64
           S+PA P   PNFWG  PEEE+YTSQGVRN+KS+FETP+GKLFTQSF+PL   E+KGTVYM
Sbjct: 11  SAPATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLDG-EIKGTVYM 70

Query: 65  THGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLH 124
           +HGYGSD  WMFQKIC+S++SWGYAVFAADLLGHGRSDGIRCY+GDM+K+AA SL+FF H
Sbjct: 71  SHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKH 130

Query: 125 TRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLF 184
            R S+PY+ LPAFLFGESMGG  T+LMYFQS+P+TWTGL+FSAPLFVIPE+MKPSK HLF
Sbjct: 131 VRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLF 190

Query: 185 LYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYI 244
            YGLLFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+
Sbjct: 191 AYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYV 250

Query: 245 RDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENM 304
           ++NF KVT P  T HGTAD V CP+SS+LLYEKA+S DK+LK+Y+GMYHSLIQGEPDEN 
Sbjct: 251 QENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENA 310

Query: 305 EIVLRDMKEWIDERVLRYGPK 325
           EIVL+DM+EWIDE+V +YG K
Sbjct: 311 EIVLKDMREWIDEKVKKYGSK 330

BLAST of HG10002246 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-21
Identity = 73/258 (28.29%), Postives = 118/258 (45.74%), Query Frame = 0

Query: 58  KGTVYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAAS 117
           +G V + HGY   AG  +  +   + + G  V+A D  GHGRS G R +L D+ +     
Sbjct: 31  RGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLVYALDHRGHGRSGGKRVHLRDLSEFVEDF 90

Query: 118 LSFFLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKP 177
            +         P   LP  + G SMGG   +  Y    P  ++ ++ S P     + + P
Sbjct: 91  RTLVGIAANDHP--TLPRIVLGHSMGG-GIVFAYGARYPGEYSAMVLSGPAVNAHDGVSP 150

Query: 178 SKLHLFLYGLLFGVADTWAAMPDNKMVGKAI-KDPEKLKIIAANPRRYTGPPRVGTMREL 237
                 L  +   +      +P   +   A+ +DPE +    A+P  + G    G  R L
Sbjct: 151 -----VLVAVAKVLGKLAPGIPVENLDADAVSRDPEVVAAYKADPMVHHGKLPAGIARAL 210

Query: 238 VRVTQYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQ 297
           + + Q +    + +TAP L VHG  D +   + S LL ++ AS D  LK+Y G+YH +  
Sbjct: 211 IGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDRVASEDVHLKVYPGLYHEVF- 270

Query: 298 GEPDENMEIVLRDMKEWI 315
            EP++  ++VL D+  WI
Sbjct: 271 NEPEQ--KLVLDDVTSWI 276

BLAST of HG10002246 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.5e-18
Identity = 89/331 (26.89%), Postives = 145/331 (43.81%), Query Frame = 0

Query: 1   MPPESSP--APETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELK 60
           MP ESSP   P+++P    +Q       + G              LF + + P  +P  K
Sbjct: 1   MPEESSPRRTPQSIP----YQDLPHLVNADG------------QYLFCRYWKPTGTP--K 60

Query: 61  GTVYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASL 120
             ++++HG G  +G  ++++          VFA D +GHG+S+G R  + D        L
Sbjct: 61  ALIFVSHGAGEHSG-RYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVL 120

Query: 121 SFFLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVI-PENMKP 180
                    + Y  LP FL G SMGGA  +L   +  P  + G++  +PL +  PE+   
Sbjct: 121 QHV--DSMQKDYPGLPVFLLGHSMGGAIAILTAAER-PGHFAGMVLISPLVLANPESATT 180

Query: 181 SK------LHLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVG 240
            K      L+L L  L  G  D+ + +  NK          ++ I  ++P       +V 
Sbjct: 181 FKVLAAKVLNLVLPNLSLGPIDS-SVLSRNK---------TEVDIYNSDPLICRAGLKVC 240

Query: 241 TMRELVRVTQYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMY 300
              +L+     +     K+T PFL + G+AD +     + LL E A S DK+LK+Y+G Y
Sbjct: 241 FGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAY 298

Query: 301 HSLIQGEPDENMEIVLRDMKEWIDERVLRYG 323
           H ++  E  E    V  ++  W+ +R    G
Sbjct: 301 H-VLHKELPEVTNSVFHEINMWVSQRTATAG 298

BLAST of HG10002246 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.0e-18
Identity = 74/282 (26.24%), Postives = 138/282 (48.94%), Query Frame = 0

Query: 44  LFTQSFIPLHSPELKGTVYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGI 103
           LF + + P  +P  K  ++++HG G   G  + ++          VFA D +GHG+S+G 
Sbjct: 30  LFCRYWKPSGTP--KALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVGHGQSEGE 89

Query: 104 RCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLI 163
           R  + D  ++    L   ++T + + Y  +P FL G SMGGA ++L   +  P  ++G+I
Sbjct: 90  RMVVSDF-QVFVRDLLQHVNTVQKD-YPEVPVFLLGHSMGGAISILAAAER-PTHFSGMI 149

Query: 164 FSAPLFVI-PENMKPSKLHLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPR 223
             +PL +  PE+    K+ L    L F + +      D+ ++    ++  ++ +  ++P 
Sbjct: 150 LISPLILANPESASTLKV-LAAKLLNFVLPNISLGRIDSSVLS---RNKSEVDLYNSDPL 209

Query: 224 RYTGPPRVGTMRELVRVTQYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDK 283
                 +V    +L+     +     ++T PFL + G+AD +     + LL E + S DK
Sbjct: 210 ICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDK 269

Query: 284 SLKLYDGMYHSLIQGEPDENMEIVLRDMKEWIDERVLRYGPK 325
           +LK+Y+G YH ++  E  E    VL ++  W+  R+   G +
Sbjct: 270 TLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHRIAVAGAR 300

BLAST of HG10002246 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.6e-18
Identity = 72/280 (25.71%), Postives = 133/280 (47.50%), Query Frame = 0

Query: 44  LFTQSFIPLHSPELKGTVYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGI 103
           LF + + P  +P  K  ++++HG G   G  + ++          VFA D +GHG+S+G 
Sbjct: 30  LFCRYWKPSGTP--KALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQSEGE 89

Query: 104 RCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLI 163
           R  + D        L      ++   Y  +P FL G SMGGA ++L+  +  P  ++G++
Sbjct: 90  RMVVSDFQVFVRDVLQHVDTIQKD--YPDVPIFLLGHSMGGAISILVAAER-PTYFSGMV 149

Query: 164 FSAPLFVI-PENMKPSKLHLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPR 223
             +PL +  PE+    K+ L    L F + +      D+ ++    ++  ++ +  ++P 
Sbjct: 150 LISPLVLANPESASTLKV-LAAKLLNFVLPNMTLGRIDSSVLS---RNKSEVDLYNSDPL 209

Query: 224 RYTGPPRVGTMRELVRVTQYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDK 283
                 +V    +L+     +     ++T PFL + G+AD +     + LL E + S DK
Sbjct: 210 VCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDK 269

Query: 284 SLKLYDGMYHSLIQGEPDENMEIVLRDMKEWIDERVLRYG 323
           +LK+Y+G YH ++  E  E    VL ++  W+  R+   G
Sbjct: 270 TLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHRIAAAG 298

BLAST of HG10002246 vs. ExPASy TrEMBL
Match: A0A5D3DA65 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00400 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.1e-176
Identity = 300/317 (94.64%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 9   PETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYG 68
           PE   NFWG QPEEEFY SQGVRNTKSFFETPHGKLFTQSFIPL SP+LKGTVYMTHGYG
Sbjct: 3   PEPPLNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTVYMTHGYG 62

Query: 69  SDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSE 128
           SD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDMDKIAAASLSFFLHTRRSE
Sbjct: 63  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 122

Query: 129 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLL 188
           PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL LFLYGLL
Sbjct: 123 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLL 182

Query: 189 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 248
           FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS
Sbjct: 183 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 242

Query: 249 KVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLR 308
           KVTAPFLTVHGTAD V CPSSS+LLYEKAASVDK+LKLYDGMYHSLIQGEPDEN+EIVLR
Sbjct: 243 KVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDENVEIVLR 302

Query: 309 DMKEWIDERVLRYGPKK 326
           DM+EWIDER LRYGPKK
Sbjct: 303 DMREWIDERALRYGPKK 319

BLAST of HG10002246 vs. ExPASy TrEMBL
Match: A0A1S3BHJ4 (caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103489918 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.1e-176
Identity = 300/317 (94.64%), Postives = 306/317 (96.53%), Query Frame = 0

Query: 9   PETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYG 68
           PE   NFWG QPEEEFY SQGVRNTKSFFETPHGKLFTQSFIPL SP+LKGTVYMTHGYG
Sbjct: 3   PEPPLNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTVYMTHGYG 62

Query: 69  SDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSE 128
           SD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDMDKIAAASLSFFLHTRRSE
Sbjct: 63  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 122

Query: 129 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLL 188
           PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL LFLYGLL
Sbjct: 123 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLL 182

Query: 189 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 248
           FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS
Sbjct: 183 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 242

Query: 249 KVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLR 308
           KVTAPFLTVHGTAD V CPSSS+LLYEKAASVDK+LKLYDGMYHSLIQGEPDEN+EIVLR
Sbjct: 243 KVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDENVEIVLR 302

Query: 309 DMKEWIDERVLRYGPKK 326
           DM+EWIDER LRYGPKK
Sbjct: 303 DMREWIDERALRYGPKK 319

BLAST of HG10002246 vs. ExPASy TrEMBL
Match: A0A6J1GBG1 (caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111452643 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.6e-175
Identity = 301/325 (92.62%), Postives = 311/325 (95.69%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           M PE + APETL N+WG QPEEEFY SQGVRN+KSFFETPHGKLFTQSFIPL SPE KGT
Sbjct: 1   MAPEPA-APETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDSPEPKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDM+K+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKAASVDK+LKLY+GMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           ENMEIVLRDM+EWIDERV RYG KK
Sbjct: 301 ENMEIVLRDMREWIDERVQRYGSKK 324

BLAST of HG10002246 vs. ExPASy TrEMBL
Match: A0A0A0LBT8 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G539670 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.0e-175
Identity = 300/325 (92.31%), Postives = 307/325 (94.46%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           MP ESSP PE   NFWG QPEEEFY SQGVRNTKSFFET HGKLFTQSFIPL  P+LKGT
Sbjct: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDMDKIAAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDP+KLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPQKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKA SVDK+LKLYDGMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           EN+EIVLRDM+EWIDER LRYGPKK
Sbjct: 301 ENVEIVLRDMREWIDERALRYGPKK 325

BLAST of HG10002246 vs. ExPASy TrEMBL
Match: A0A6J1K763 (caffeoylshikimate esterase OS=Cucurbita maxima OX=3661 GN=LOC111492898 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.0e-174
Identity = 300/325 (92.31%), Postives = 309/325 (95.08%), Query Frame = 0

Query: 1   MPPESSPAPETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGT 60
           M PE  PAPETL N+WG QPEEEFY SQGVRN+KSFFETPHGKLFTQSFIPL  PE KGT
Sbjct: 1   MAPE-PPAPETLTNYWGDQPEEEFYASQGVRNSKSFFETPHGKLFTQSFIPLDWPEPKGT 60

Query: 61  VYMTHGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSF 120
           VYMTHGYGSD GWMFQKICLSYASWGYAVFAADLLGHGRSDG+RCYLGDM+K+AAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPY HLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYCHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 HLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPD 300
           QYIRDNFS+VTAPFLTVHGTAD V CPSSSELLYEKAASVDK+LKLY+GMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKAASVDKTLKLYEGMYHSLIQGEPD 300

Query: 301 ENMEIVLRDMKEWIDERVLRYGPKK 326
           ENMEIVLRDM+EWIDERV RYG KK
Sbjct: 301 ENMEIVLRDMREWIDERVQRYGSKK 324

BLAST of HG10002246 vs. TAIR 10
Match: AT1G52760.1 (lysophospholipase 2 )

HSP 1 Score: 537.7 bits (1384), Expect = 6.3e-153
Identity = 249/321 (77.57%), Postives = 288/321 (89.72%), Query Frame = 0

Query: 5   SSPA-PETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYM 64
           S+PA P   PNFWG  PEEE+YTSQGVRN+KS+FETP+GKLFTQSF+PL   E+KGTVYM
Sbjct: 11  SAPATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLDG-EIKGTVYM 70

Query: 65  THGYGSDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLH 124
           +HGYGSD  WMFQKIC+S++SWGYAVFAADLLGHGRSDGIRCY+GDM+K+AA SL+FF H
Sbjct: 71  SHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKH 130

Query: 125 TRRSEPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLF 184
            R S+PY+ LPAFLFGESMGG  T+LMYFQS+P+TWTGL+FSAPLFVIPE+MKPSK HLF
Sbjct: 131 VRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLF 190

Query: 185 LYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYI 244
            YGLLFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+
Sbjct: 191 AYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYV 250

Query: 245 RDNFSKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENM 304
           ++NF KVT P  T HGTAD V CP+SS+LLYEKA+S DK+LK+Y+GMYHSLIQGEPDEN 
Sbjct: 251 QENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENA 310

Query: 305 EIVLRDMKEWIDERVLRYGPK 325
           EIVL+DM+EWIDE+V +YG K
Sbjct: 311 EIVLKDMREWIDEKVKKYGSK 330

BLAST of HG10002246 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.6e-71
Identity = 134/310 (43.23%), Postives = 195/310 (62.90%), Query Frame = 0

Query: 10  ETLPNFWGHQPEEEFYTSQGVRNTKSFFETPHG-KLFTQSFIPLHSPELKGTVYMTHGYG 69
           ET  NFWG   EE+++  +G+  +KSFF +P G  LFT+S++P  S   +G ++M HGYG
Sbjct: 7   ETEINFWGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYG 66

Query: 70  SDAGWMFQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSE 129
           +D  W FQ   +  A  G+A FA D+ GHGRSDG+R Y+  +D +    +SFF   +++ 
Sbjct: 67  NDVSWTFQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIKQNP 126

Query: 130 PYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPS-KLHLFLYGL 189
            ++ LP FLFGESMGGA  +L+ F +DP  + G +  AP+  I + ++P   +  FL  +
Sbjct: 127 KFQGLPRFLFGESMGGAICLLIQF-ADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLI-M 186

Query: 190 LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF 249
           +     TWA +P   ++ K+IK  EK  I   NP RY   PR+GT+ EL+RVT Y+    
Sbjct: 187 ISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKPRLGTVMELLRVTDYLGKKL 246

Query: 250 SKVTAPFLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVL 309
             V+ PF+ VHG+AD V  P  S  LYE A S DK+LK+YDGM HS++ GEPD+N+EIV 
Sbjct: 247 KDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVR 306

Query: 310 RDMKEWIDER 318
           +D+  W+++R
Sbjct: 307 KDIVSWLNDR 314

BLAST of HG10002246 vs. TAIR 10
Match: AT3G62860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.7e-52
Identity = 102/293 (34.81%), Postives = 158/293 (53.92%), Query Frame = 0

Query: 25  YTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYGSDAGWMFQKICLSYAS 84
           Y  + ++N++        +LF   ++P  SP  +  V++ HGYG +     ++  +  AS
Sbjct: 8   YEEEYIKNSRDV------ELFACRWLPSSSP--RALVFLCHGYGMECSSFMRECGIRLAS 67

Query: 85  WGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGG 144
            GYAVF  D  GHGRS G RCY+     I      ++      E Y+    FL+GESMGG
Sbjct: 68  AGYAVFGMDYEGHGRSKGARCYIKKFSNIVNDCFDYYTSISAQEEYKEKGRFLYGESMGG 127

Query: 145 AATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLLFGVADTWAAMPDNKMV 204
           A  +L++ + DP  W G +  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVALLLH-KKDPSFWNGALLVAPMCKISEKVKPHPVVINLLTRVEDIIPKWKIVPTKDVI 187

Query: 205 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSKVTAPFLTVHGTADVV 264
             A KDP K + I  N   Y   PR+ T  E++R +  + D   ++T PF  +HG AD+V
Sbjct: 188 DAAFKDPVKREEIRNNKLIYQDKPRLKTALEMLRTSMDLEDTLHEITLPFFVLHGEADIV 247

Query: 265 ACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLRDMKEWIDER 318
             P  S+ L+EKA++ DK++KLY GM+H L  GEPD N+++V  D+  W+D R
Sbjct: 248 TDPEISKALFEKASTRDKTIKLYPGMWHGLTSGEPDANVDLVFADIVNWLDAR 291

BLAST of HG10002246 vs. TAIR 10
Match: AT2G47630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.2e-50
Identity = 106/293 (36.18%), Postives = 156/293 (53.24%), Query Frame = 0

Query: 25  YTSQGVRNTKSFFETPHGKLFTQSFIPLHSPELKGTVYMTHGYGSDAGWMFQKICLSYAS 84
           Y  + VRN++        +LF   +IP  S   K  V++ HGYG +     ++  +  AS
Sbjct: 8   YHEEYVRNSRGV------ELFACRWIPSSSSSPKALVFLCHGYGMECSDSMKECGIRLAS 67

Query: 85  WGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGG 144
            GYAVF  D  GHGRS G RCY+     +      ++      E Y     FL+GESMGG
Sbjct: 68  AGYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSICAQEEYMDKGRFLYGESMGG 127

Query: 145 AATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLLFGVADTWAAMPDNKMV 204
           A T+L++ + DP  W G I  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVTLLLH-KKDPLFWNGAILVAPMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVI 187

Query: 205 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSKVTAPFLTVHGTADVV 264
             A KD  K + +  N   Y   PR+ T  E++R +  + D   ++T PF  +HG AD V
Sbjct: 188 DAAFKDLVKREEVRNNKLIYQDKPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTV 247

Query: 265 ACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLRDMKEWIDER 318
             P  S+ LYEKA++ DK+LKLY GM+H+L  GEPD N+++V  D+  W+D R
Sbjct: 248 TDPEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWLDLR 293

BLAST of HG10002246 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.6e-50
Identity = 111/304 (36.51%), Postives = 165/304 (54.28%), Query Frame = 0

Query: 16  WGHQPEEEFYTSQGVRNTKSFFETPHG-KLFTQSFIPLHSPELKGTVYMTHGYGSDAGWM 75
           +G     EFY+   V ++ ++   P G KLFTQ + PL+ P L G + + HG+  ++ W 
Sbjct: 15  FGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIAVVHGFTGESSWF 74

Query: 76  FQKICLSYASWGYAVFAADLLGHGRSDGIRCYLGDMDKIAAASLSFFLHTRRSEPYRHLP 135
            Q   + +A  GY   A D  GHG SDG+  ++ +++ I    +SFF   R+      LP
Sbjct: 75  LQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFDDFRKRHASSFLP 134

Query: 136 AFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLHLFLYGLLFGVADT 195
           AFL+ ES+GGA  + +  +     W GLI S  +  I    KP      L  L   +  T
Sbjct: 135 AFLYSESLGGAIALYITLR-QKHQWNGLILSGAMCSISHKFKPPWPLQHLLTLAATLIPT 194

Query: 196 WAAMPD-NKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSKVTAP 255
           W  +P    + G + K+P K K+  ANP R  G PR  T  ELVRV + +++ F +V  P
Sbjct: 195 WRVVPTRGSIAGVSFKEPWKRKLAYANPNRTVGKPRAATAYELVRVCEDLQNRFEEVEVP 254

Query: 256 FLTVHGTADVVACPSSSELLYEKAASVDKSLKLYDGMYHSLIQGEPDENMEIVLRDMKEW 315
            + VHG  DVV  P+S E LY + +S DK++K+Y GM+H LI GE +EN+++V  D+ +W
Sbjct: 255 LMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLI-GESEENVDLVFGDVLDW 314

Query: 316 IDER 318
           I  R
Sbjct: 315 IKTR 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874501.11.8e-17893.23LOW QUALITY PROTEIN: caffeoylshikimate esterase [Benincasa hispida][more]
XP_008447481.18.5e-17694.64PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0038136.1 caffeoylshiki... [more]
XP_023525534.13.2e-17592.62caffeoylshikimate esterase [Cucurbita pepo subsp. pepo][more]
XP_022949228.13.2e-17592.62caffeoylshikimate esterase [Cucurbita moschata][more]
KAG6606779.14.2e-17592.31Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
Match NameE-valueIdentityDescription
Q9C9428.9e-15277.57Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
A0QNZ71.9e-2128.29Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(... [more]
Q996851.5e-1826.89Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
Q8R4312.0e-1826.24Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1[more]
O356787.6e-1825.71Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DA654.1e-17694.64Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BHJ44.1e-17694.64caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103489918 PE=4 SV=1[more]
A0A6J1GBG11.6e-17592.62caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111452643 PE=4 SV... [more]
A0A0A0LBT86.0e-17592.31Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G539670... [more]
A0A6J1K7633.0e-17492.31caffeoylshikimate esterase OS=Cucurbita maxima OX=3661 GN=LOC111492898 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52760.16.3e-15377.57lysophospholipase 2 [more]
AT1G11090.11.6e-7143.23alpha/beta-Hydrolases superfamily protein [more]
AT3G62860.13.7e-5234.81alpha/beta-Hydrolases superfamily protein [more]
AT2G47630.11.2e-5036.18alpha/beta-Hydrolases superfamily protein [more]
AT5G14980.12.6e-5036.51alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 137..150
score: 41.63
coord: 86..101
score: 41.81
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 23..319
e-value: 3.1E-48
score: 166.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 23..318
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 56..299
e-value: 8.4E-50
score: 169.3
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 15..323
NoneNo IPR availablePANTHERPTHR11614:SF163CAFFEOYLSHIKIMATE ESTERASEcoord: 15..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002246.1HG10002246.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity