HG10002211 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002211
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationChr11: 4534403 .. 4537410 (-)
RNA-Seq ExpressionHG10002211
SyntenyHG10002211
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGGGCCTCAACTACAAGGGCACAAACTAATGGATGCATCAAGAGAAACAAAGAGAATTGTGAAGCCTCCAATGTCATTTGGGAAGAGAAAAAGGATCAAGATAGACAAAAAAGTGGCTTGTGTGTATTTCTTTTTGCATTTGCTTTGTATTTTCGCACCATTTCAAAGCAATTGGAGGGCATTCTCGGTTTGTTTAGCATTATTTATGATCACCGGTCTATTTGGCATCACTATTTCGTATCACCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATGGCTTGAGTATTTCTTTGCTTATTGCGGAGTTCATGCACTTCAGGTTTGACACTTGATGTTTGTATTTTTTACTCTTATAAGTGTAACTTTCATGTATTAATGATTGATTTAAAAACTAATCGATGGATCTTAAAGCATATCAAGATATATATGTTGTCTTTAAATTTTTTAATATATTTTTTTTATACATATTAGGGTGATCCAATCAATTGGGTGAGCACGCATAGATATCATCATCAATTTGTTGATACCGAAAAAGATCCACATAGTCCTGTTCAAGGATTTTGGTTTAGTTATATTATTTGGATTCTCGAAGAAAGAGATGGAAGAATCATTGAAATGGTTAGGAAGCATCGAAAATTGGATAATGCTGGCGACTTAGAGAAGCAAACTTTCTATAGGTTTATTCGCAAAACATATTTACTTCATCCAATTGCTGTTGCAGTCATACTTTATGTCATTGGAGGAATTCCCTTTTTCATTTGGGGAACGGTAAAAATTAAAAAAAAAAAAAAAGTTTAAAAATCTCTCTCCTTCATCATTTGTTTAATTCCTGTTAGTTATAATCTATATGTTATGGTAACTTGATTAATAAAACTTGATTTAAGTTTTTAAAAAAATATATTTCAATTATATTTAATGAAATAAGACTCCTGATTATTTTAATTTTAGCTTTCTGTTTTTGAAGACTAATCTGTTTATTTAATCATTTCTCCCAAATATGAATAGTTTAGATGCAAAATACGGTTCTTTTCATTTAAAAAAAAAAAAAAAAAAAAAAAATAATCTGTATTTTTGTATAAATGTATATATGTAGGTTAAATTACAAGGATGTTTAGTCTCTAAACGCTTAGGTTTATAAATTTTTAAAATGAATGTCCTTCAACTTTTAATTTCATGTCTAATAAGTTTGTAATAACTTTAAATTTTGTGTCCGATAAATTTCTAAACTTTCAATATTCTAATTAGTAAAACTCTAAAATGTTAATTTTATGTTTAATAGATATGTGAATAAAAAAAATAGGTAGATAGATTAAAGACCTATTAGATACAAATTAAGTTGTAAGATTTAGGTATATATGTTGGTTCCATGGCATCACGAGGATGTTAAGAATGTGTTAATCTAGTTTAGATATCCCGATGCACCTACAGATCTCCCTTCTAATATTTTTGAAAATTTGAAGATCTATTAGATATTTTGAAAGTCTATGATTTAATAAACATAAAAGTGAATGTTTAACAATCTATTAGATACTCTCTAAATTTTAGGTTTTTAATTTTGATTTTAAAAAATTTAAAAGTTTAATGACTTATTAGACAATCTTGATCTTGAAACTTTTTATTGGACAGTGTGTGAGAACCGTAATGCTTTTACATGTAACATTTATGGTGAATTCATTATGTCATTTGTGGGGAAAGCAACCATGGAAAACAAACGATTTCTCTAGAAACAATTGGTAAGTATGTTGCACCTTTCTTTGTATCAACAATGTTGAGTAAAAGATGATCAAATTAATTTATTGAATGCGTTTTTGCTTCCATAATAATATTCTTTTAGATGATAAATTTATGAAAAATTATTTTAAATGACAAAATTGTTAAAAGTATTAATAAATATAATAAAATTTTACTGTCTATCTATTATAGACTGCAATAGACTACTACTATCTATATAGCGATAGACTATAGATAGATACAGACAGTAGTCTATTGCGGTATATCGCAAATAGGCAGTAAAATTTTACTATATTTATAAATATTTTGGTTTATGTAGAGATTTATTATATATATAATATATATAATATATATATATTAATATAATATATATATTATCCATGATATATAACTATTGTTTTGCGAATAAGACACCAATTATCATCTTAAAAGGTGATACTATATATATATATACATATATATATATATATACGTACGTATATATAATATATTGTAACTTTTAATATAAAATCTATTTAATTTAATACATTGTTGGACCATTTTGTGATTCCTAAAATCAAAATCAAAATATCTATCGAATCGTTTCTACATTTCATACATGGATTAATAAACCTTGTGCTCTTGTTATATAGATATTACAATATAGATTTTTTTTTAAAGATTTATTGAAAGTATACGACTAAATTTAGGTATTTAAAAGTCGAGAAGTAAAATAGAACAAATCTCTACATAGATAGACTAAAATATTTTAAACTAAAAAAAAATCTATATTGTAAGGGACAAAAATCTACATAGATAGATGTGAGAGAAACAAAACCCCGAGTTTACTCGATAAGGAAAATATATTCGACTAGAATCAGCACTTTGAGAGACATCCTATGAAACATTTCAAGAGACAGACGCACCAACCTCTTAAGGGACAACTGTCAAAACAACTTTAAAAAATATAGTCAATATCAAAACTCTAACCATAATAAAATTCTTCAATAATTATTAAATTTTATGTTTATTAAGCAATTTACTTCAATATAAGTATAATAAATTTCTTTTTCTTTTCAAAGGTGGATGAGTCTTCTTGCATTTGGAGAAGGTTGGCACAATAACCATCATGCTTTCGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACCTGGGTTTGTATGTTATTGTGTTTCTTCAAGCCATTGGAGTGGCCACTAATGTGAAATTACCATCTCAAAGCCATAAGCAAAAGTTGGCCAAAGATAAACTATTATAA

mRNA sequence

ATGCAAGGGCCTCAACTACAAGGGCACAAACTAATGGATGCATCAAGAGAAACAAAGAGAATTGTGAAGCCTCCAATGTCATTTGGGAAGAGAAAAAGGATCAAGATAGACAAAAAAGTGGCTTGTGTGTATTTCTTTTTGCATTTGCTTTGTATTTTCGCACCATTTCAAAGCAATTGGAGGGCATTCTCGGTTTGTTTAGCATTATTTATGATCACCGGTCTATTTGGCATCACTATTTCGTATCACCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATGGCTTGAGTATTTCTTTGCTTATTGCGGAGTTCATGCACTTCAGGGTGATCCAATCAATTGGGTGAGCACGCATAGATATCATCATCAATTTGTTGATACCGAAAAAGATCCACATAGTCCTGTTCAAGGATTTTGGTTTAGTTATATTATTTGGATTCTCGAAGAAAGAGATGGAAGAATCATTGAAATGGTTAGGAAGCATCGAAAATTGGATAATGCTGGCGACTTAGAGAAGCAAACTTTCTATAGGTTTATTCGCAAAACATATTTACTTCATCCAATTGCTGTTGCAGTCATACTTTATGTCATTGGAGGAATTCCCTTTTTCATTTGGGGAACGTGTGTGAGAACCGTAATGCTTTTACATGTAACATTTATGGTGAATTCATTATGTCATTTGTGGGGAAAGCAACCATGGAAAACAAACGATTTCTCTAGAAACAATTGGTGGATGAGTCTTCTTGCATTTGGAGAAGGTTGGCACAATAACCATCATGCTTTCGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACCTGGGTTTGTATGTTATTGTGTTTCTTCAAGCCATTGGAGTGGCCACTAATGTGAAATTACCATCTCAAAGCCATAAGCAAAAGTTGGCCAAAGATAAACTATTATAA

Coding sequence (CDS)

ATGCAAGGGCCTCAACTACAAGGGCACAAACTAATGGATGCATCAAGAGAAACAAAGAGAATTGTGAAGCCTCCAATGTCATTTGGGAAGAGAAAAAGGATCAAGATAGACAAAAAAGTGGCTTGTGTGTATTTCTTTTTGCATTTGCTTTGTATTTTCGCACCATTTCAAAGCAATTGGAGGGCATTCTCGGTTTGTTTAGCATTATTTATGATCACCGGTCTATTTGGCATCACTATTTCGTATCACCGAAATCTTTCACATAAGAGTTTCAAAATTCCCAAATGGCTTGAGTATTTCTTTGCTTATTGCGGAGTTCATGCACTTCAGGGTGATCCAATCAATTGGGTGAGCACGCATAGATATCATCATCAATTTGTTGATACCGAAAAAGATCCACATAGTCCTGTTCAAGGATTTTGGTTTAGTTATATTATTTGGATTCTCGAAGAAAGAGATGGAAGAATCATTGAAATGGTTAGGAAGCATCGAAAATTGGATAATGCTGGCGACTTAGAGAAGCAAACTTTCTATAGGTTTATTCGCAAAACATATTTACTTCATCCAATTGCTGTTGCAGTCATACTTTATGTCATTGGAGGAATTCCCTTTTTCATTTGGGGAACGTGTGTGAGAACCGTAATGCTTTTACATGTAACATTTATGGTGAATTCATTATGTCATTTGTGGGGAAAGCAACCATGGAAAACAAACGATTTCTCTAGAAACAATTGGTGGATGAGTCTTCTTGCATTTGGAGAAGGTTGGCACAATAACCATCATGCTTTCGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACCTGGGTTTGTATGTTATTGTGTTTCTTCAAGCCATTGGAGTGGCCACTAATGTGAAATTACCATCTCAAAGCCATAAGCAAAAGTTGGCCAAAGATAAACTATTATAA

Protein sequence

MQGPQLQGHKLMDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKLL
Homology
BLAST of HG10002211 vs. NCBI nr
Match: XP_038903351.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida] >XP_038903358.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 564.7 bits (1454), Expect = 5.0e-157
Identity = 264/300 (88.00%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDASRE+K  VK  MSF K+K+IKIDKKVA V+FFLHL CIFAPFQ NW AFS+CLALFM
Sbjct: 1   MDASRESKINVKSRMSFQKKKKIKIDKKVAGVFFFLHLCCIFAPFQINWGAFSICLALFM 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGITISYHRNLSHKSFKIPK+LEY FAYCGVHALQGDPINWVS HRYHHQFVDTEK
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKYLEYLFAYCGVHALQGDPINWVSKHRYHHQFVDTEK 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP+ GFWFSYIIW +E RDG IIE  RKHRKLDNAGDLEKQTFYRFIRKTY+LHPIA
Sbjct: 121 DPHSPILGFWFSYIIWAVEGRDGSIIETNRKHRKLDNAGDLEKQTFYRFIRKTYILHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY +GGIPFFIWGTCVRTVMLLH TFMVNS CH+WGKQPWKTNDFS NNWWMSLLA
Sbjct: 181 LALILYFLGGIPFFIWGTCVRTVMLLHSTFMVNSFCHMWGKQPWKTNDFSTNNWWMSLLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 311
           FGEGWHNNHHAFEYSARMGIEWWQFDLG YVIVFLQAIGVATNVK+PSQSHKQKLAKDKL
Sbjct: 241 FGEGWHNNHHAFEYSARMGIEWWQFDLGWYVIVFLQAIGVATNVKVPSQSHKQKLAKDKL 300

BLAST of HG10002211 vs. NCBI nr
Match: XP_038903333.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 547.7 bits (1410), Expect = 6.3e-152
Identity = 252/290 (86.90%), Postives = 268/290 (92.41%), Query Frame = 0

Query: 22  VKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITIS 81
           VK P+   K K+IKIDKKVA V+FFLH  CIFAPFQ NW AFS+CLALFMITGLFGITIS
Sbjct: 3   VKSPILSEKEKKIKIDKKVAGVFFFLHFCCIFAPFQINWGAFSICLALFMITGLFGITIS 62

Query: 82  YHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFW 141
           YHRNLSHKSFK+PK+LEY FAYCGVHALQGDPI WVSTHRYHHQFVDT KDPHSPV+GFW
Sbjct: 63  YHRNLSHKSFKVPKYLEYLFAYCGVHALQGDPITWVSTHRYHHQFVDTIKDPHSPVRGFW 122

Query: 142 FSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGG 201
           F YIIW++EE+DG IIE  RKHRKLDNAGDLEKQTFYRFIRKTY+LHPIA+A+ILY +GG
Sbjct: 123 FGYIIWVVEEKDGSIIETNRKHRKLDNAGDLEKQTFYRFIRKTYILHPIALALILYFLGG 182

Query: 202 IPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHH 261
           IPFFIWGTCVRTVMLLH TFMVNS CH+WGKQPWKTNDFS NNWWMSLLAFGEGWHNNHH
Sbjct: 183 IPFFIWGTCVRTVMLLHSTFMVNSFCHMWGKQPWKTNDFSTNNWWMSLLAFGEGWHNNHH 242

Query: 262 AFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 312
           AFEYSARMGIEWWQFDLG YVIVFLQAIGVATNVK+PSQSHKQKLAKDKL
Sbjct: 243 AFEYSARMGIEWWQFDLGWYVIVFLQAIGVATNVKVPSQSHKQKLAKDKL 292

BLAST of HG10002211 vs. NCBI nr
Match: XP_008454220.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 538.1 bits (1385), Expect = 5.0e-149
Identity = 247/298 (82.89%), Postives = 267/298 (89.60%), Query Frame = 0

Query: 12  MDA-SRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALF 71
           MDA SR+  + VKPPMSFGKRKR KIDKKVA V+  LHLLCIFAPFQ NW AFS+CL LF
Sbjct: 1   MDALSRDPMKTVKPPMSFGKRKRNKIDKKVASVFLLLHLLCIFAPFQINWGAFSICLVLF 60

Query: 72  MITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTE 131
            IT LFGITISYHRNLSHKSF+IPKWLEY FAYCGVHALQG+PINWVSTHRYHHQFVDTE
Sbjct: 61  TITSLFGITISYHRNLSHKSFQIPKWLEYLFAYCGVHALQGNPINWVSTHRYHHQFVDTE 120

Query: 132 KDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPI 191
           +DPH+P+ GFWF YIIWILEE+DG I  M RKHRKLDNA DLEKQ FYRFIRKTYLLHPI
Sbjct: 121 QDPHTPIHGFWFGYIIWILEEKDGSIAGMNRKHRKLDNASDLEKQIFYRFIRKTYLLHPI 180

Query: 192 AVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLL 251
           A+A+ILYVIGG PFFIWGTCVRTVMLLH TFMVNS CH+WG QPWKTNDFS NNWW+SL+
Sbjct: 181 ALAIILYVIGGFPFFIWGTCVRTVMLLHSTFMVNSFCHMWGNQPWKTNDFSANNWWVSLV 240

Query: 252 AFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAK 309
            FGEGWHNNHHAFEYSAR+GIEWWQFD+G YVI+FLQAIG+ATNVKLPSQSH  KLA+
Sbjct: 241 TFGEGWHNNHHAFEYSARLGIEWWQFDIGWYVIMFLQAIGLATNVKLPSQSHMHKLAQ 298

BLAST of HG10002211 vs. NCBI nr
Match: XP_022955957.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata] >XP_022955958.1 palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-144
Identity = 239/300 (79.67%), Postives = 267/300 (89.00%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDASR+ KRIVKPPMSFGK++  +IDK+VA  +F LH LCIFAPFQ N  A S+C ALF+
Sbjct: 1   MDASRDPKRIVKPPMSFGKKRWTEIDKRVAVAFFVLHFLCIFAPFQMNRGALSICFALFV 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGITISYHRNLSHKSFKIPKWLEY FAYCGVHA QGDPINWVSTHRYHHQFVDTE+
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKWLEYSFAYCGVHAFQGDPINWVSTHRYHHQFVDTEQ 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP+ GFWFSYI W L E +G I EM+ +HRKLDNA DLEKQTFYRFIR+TY+LHPIA
Sbjct: 121 DPHSPIHGFWFSYITWFLYESNGSISEMIGRHRKLDNACDLEKQTFYRFIRETYVLHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGG P+FIWGTCVRT+MLLH T++VNSLCH+WGKQPWKTND S NNW MS LA
Sbjct: 181 LALILYGIGGFPYFIWGTCVRTIMLLHTTYLVNSLCHMWGKQPWKTNDLSTNNWCMSFLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 311
           FGEGWHNNHHAFEYSARMGIEWWQ+D G YVIVFLQAIGVAT+VKLPS++HK+KLAK+ L
Sbjct: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWYVIVFLQAIGVATHVKLPSETHKKKLAKEYL 300

BLAST of HG10002211 vs. NCBI nr
Match: XP_023528390.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 520.8 bits (1340), Expect = 8.3e-144
Identity = 239/300 (79.67%), Postives = 264/300 (88.00%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MD SR+ KRIV PPMSFGK++  +IDKKV   +F LH LCIFAPFQ N  A S+C ALF+
Sbjct: 1   MDTSRDPKRIVNPPMSFGKKRWTEIDKKVVFAFFVLHFLCIFAPFQMNRGALSICFALFI 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGITISYHRNLSHKSFKIPKWLEY FAYCGVHA QGDPINWVSTHRYHHQFVDTE+
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKWLEYSFAYCGVHAFQGDPINWVSTHRYHHQFVDTEQ 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP+ GFWFSYI W L E +G I EM+ +HRKLDNA DLEKQTFYRFIR+TY+LHPIA
Sbjct: 121 DPHSPIHGFWFSYITWFLYESNGSISEMIGRHRKLDNACDLEKQTFYRFIRETYVLHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGG+P+FIWGTCVRTVMLLH T++VNSLCH+WGKQPWKTND S NNW MS LA
Sbjct: 181 LALILYGIGGLPYFIWGTCVRTVMLLHTTYLVNSLCHMWGKQPWKTNDLSTNNWCMSFLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 311
           FGEGWHNNHHAFEYSARMGIEWWQ+D G YVIVFLQAIGVAT+VKLPSQ+H QKLAK+ L
Sbjct: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWYVIVFLQAIGVATHVKLPSQTHMQKLAKEYL 300

BLAST of HG10002211 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-104
Identity = 166/285 (58.25%), Postives = 219/285 (76.84%), Query Frame = 0

Query: 23  KPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISY 82
           K  + + +R+   +D     V   +HLL + APFQ NWRA SV   L+++TGL GIT+S+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 83  HRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWF 142
           HRNLSHK+FK+PKWLEY FAYCG  ALQG+PI+WVSTHRYHHQF D+++DPHSP+ GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 143 SYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGI 202
           S++ W+ +        + ++  + +N GDLEKQ FYRF+R TY+LHP+A+AV LY +GG 
Sbjct: 208 SHMNWMFDTN-----TITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGF 267

Query: 203 PFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHA 262
           PF +WG  VR V + H+T++VNS CH+WGKQ W T D S+NNWW++ LAFGEGWHNNHHA
Sbjct: 268 PFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHA 327

Query: 263 FEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLA 308
           FE+SAR G+EWWQ D+  YV+ FLQAIG+AT+VKLPS++ KQ++A
Sbjct: 328 FEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of HG10002211 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 9.6e-95
Identity = 143/261 (54.79%), Postives = 202/261 (77.39%), Query Frame = 0

Query: 47  LHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGV 106
           LH+LC+FAP   +W++F VC AL+ I G+FG T+S+HRNL+H+SFK+PK+LEYFFAY G+
Sbjct: 54  LHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRSFKLPKYLEYFFAYVGL 113

Query: 107 HALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKL 166
           HALQGDP+ WVSTHRYHH++ DT  DPHSP++GFWF +I W+ + +      ++ K  + 
Sbjct: 114 HALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFDSK-----YIIEKCGRY 173

Query: 167 DNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSL 226
           +NAGDL KQ++YRF+ +T++ H    A +LY+ GG PF +WG  VRT++  H++++VNS+
Sbjct: 174 ENAGDLMKQSYYRFLERTFVYHVYLQAALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSV 233

Query: 227 CHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFL 286
           CH WG +PW T D S NNW++++L  GEGWHNNHHAFEYSAR GIEWWQ D   Y+I  L
Sbjct: 234 CHRWGNRPWNTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLL 293

Query: 287 QAIGVATNVKLPSQSHKQKLA 308
           + +G+AT++K+PS+ HK+K++
Sbjct: 294 EYLGLATDIKVPSEIHKRKMS 309

BLAST of HG10002211 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.6e-92
Identity = 154/301 (51.16%), Postives = 213/301 (70.76%), Query Frame = 0

Query: 7   QGHKLMDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVC 66
           + +K M A +      K  M   K KR+ I K  A +  F+H LC+ APF   W A  V 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASL--FVHFLCLLAPFNFTWPALRVA 69

Query: 67  LALFMITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQF 126
           L ++ + GL GIT+SYHRNL+H+SFK+PKWLEYFFAYCG+ A+QGDPI+WVSTHRYHHQF
Sbjct: 70  LIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 129

Query: 127 VDTEKDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYL 186
            D+++DPHSP +GFWFS+++W+ +   G ++E   K  +  N  DL++Q +Y+F+++T L
Sbjct: 130 TDSDRDPHSPNEGFWFSHLLWLFD--TGYLVE---KCGRRTNVEDLKRQWYYKFLQRTVL 189

Query: 187 LHPIAVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWW 246
            H +    +LY  GG+ F  WG  +   M  HVT ++NSLCH+WG + WKTND SRN WW
Sbjct: 190 YHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWW 249

Query: 247 MSLLAFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKL 306
           +S+ +FGE WHNNHHAFE SAR G+EWWQ D+  Y++ FL+ IG+AT+VKLPS+S ++++
Sbjct: 250 LSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRM 302

Query: 307 A 308
           A
Sbjct: 310 A 302

BLAST of HG10002211 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.8e-92
Identity = 145/261 (55.56%), Postives = 198/261 (75.86%), Query Frame = 0

Query: 47  LHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGV 106
           LH+LC+ APF  +W AF + L L+ + G+FG T+SYHRNL+H+SFK+PK+LEYFFAY G+
Sbjct: 51  LHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGL 110

Query: 107 HALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKL 166
           HALQGDP+ WVSTHRYHH+F DT  DPHSP++GFWFS+I W+ + +      ++ +  + 
Sbjct: 111 HALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDSK-----YILEECGRY 170

Query: 167 DNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSL 226
           +NAGDL KQ++YRF+ +T++ H    A +LY+ GG PF +WG  VRTV   H +++VNS+
Sbjct: 171 ENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGMAVRTVWGFHASWLVNSV 230

Query: 227 CHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFL 286
           CH +G Q W T D S NNW++++L  GEGWHNNHHAFEYSAR GIEWWQ D   YVI  L
Sbjct: 231 CHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLL 290

Query: 287 QAIGVATNVKLPSQSHKQKLA 308
           + +G+AT+VK+PS+ HK+K++
Sbjct: 291 EYLGLATDVKVPSEVHKRKMS 306

BLAST of HG10002211 vs. ExPASy Swiss-Prot
Match: Q9SID2 (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=2)

HSP 1 Score: 321.6 bits (823), Expect = 9.6e-87
Identity = 141/280 (50.36%), Postives = 196/280 (70.00%), Query Frame = 0

Query: 28  FGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISYHRNLS 87
           F  R+  ++D       F +H L + APF   W A  V    + I GL GIT+SYHRNL+
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLA 90

Query: 88  HKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWFSYIIW 147
           H+SFK+PKWLEY  AYC + A+QGDPI+WVSTHRYHHQF D+E+DPHSP +GFWFS+++W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 148 ILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGIPFFIW 207
           I +        +V K  +  N  DL++Q FYRF++KT L H + +   L+ +GG+ F  W
Sbjct: 151 IYDS-----AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTW 210

Query: 208 GTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHAFEYSA 267
           G  V   + +HVT ++NSLCH+WG + WKTND SRN WW+S+ +FGE WHNNHHAFE SA
Sbjct: 211 GMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSA 270

Query: 268 RMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLA 308
           R G+EWWQ D+  Y++ F + IG+AT+VK+P+++ ++++A
Sbjct: 271 RQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 304

BLAST of HG10002211 vs. ExPASy TrEMBL
Match: A0A1S3BY39 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103494692 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.4e-149
Identity = 247/298 (82.89%), Postives = 267/298 (89.60%), Query Frame = 0

Query: 12  MDA-SRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALF 71
           MDA SR+  + VKPPMSFGKRKR KIDKKVA V+  LHLLCIFAPFQ NW AFS+CL LF
Sbjct: 1   MDALSRDPMKTVKPPMSFGKRKRNKIDKKVASVFLLLHLLCIFAPFQINWGAFSICLVLF 60

Query: 72  MITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTE 131
            IT LFGITISYHRNLSHKSF+IPKWLEY FAYCGVHALQG+PINWVSTHRYHHQFVDTE
Sbjct: 61  TITSLFGITISYHRNLSHKSFQIPKWLEYLFAYCGVHALQGNPINWVSTHRYHHQFVDTE 120

Query: 132 KDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPI 191
           +DPH+P+ GFWF YIIWILEE+DG I  M RKHRKLDNA DLEKQ FYRFIRKTYLLHPI
Sbjct: 121 QDPHTPIHGFWFGYIIWILEEKDGSIAGMNRKHRKLDNASDLEKQIFYRFIRKTYLLHPI 180

Query: 192 AVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLL 251
           A+A+ILYVIGG PFFIWGTCVRTVMLLH TFMVNS CH+WG QPWKTNDFS NNWW+SL+
Sbjct: 181 ALAIILYVIGGFPFFIWGTCVRTVMLLHSTFMVNSFCHMWGNQPWKTNDFSANNWWVSLV 240

Query: 252 AFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAK 309
            FGEGWHNNHHAFEYSAR+GIEWWQFD+G YVI+FLQAIG+ATNVKLPSQSH  KLA+
Sbjct: 241 TFGEGWHNNHHAFEYSARLGIEWWQFDIGWYVIMFLQAIGLATNVKLPSQSHMHKLAQ 298

BLAST of HG10002211 vs. ExPASy TrEMBL
Match: A0A6J1GV18 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457790 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.2e-145
Identity = 239/300 (79.67%), Postives = 267/300 (89.00%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDASR+ KRIVKPPMSFGK++  +IDK+VA  +F LH LCIFAPFQ N  A S+C ALF+
Sbjct: 1   MDASRDPKRIVKPPMSFGKKRWTEIDKRVAVAFFVLHFLCIFAPFQMNRGALSICFALFV 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGITISYHRNLSHKSFKIPKWLEY FAYCGVHA QGDPINWVSTHRYHHQFVDTE+
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKWLEYSFAYCGVHAFQGDPINWVSTHRYHHQFVDTEQ 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP+ GFWFSYI W L E +G I EM+ +HRKLDNA DLEKQTFYRFIR+TY+LHPIA
Sbjct: 121 DPHSPIHGFWFSYITWFLYESNGSISEMIGRHRKLDNACDLEKQTFYRFIRETYVLHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGG P+FIWGTCVRT+MLLH T++VNSLCH+WGKQPWKTND S NNW MS LA
Sbjct: 181 LALILYGIGGFPYFIWGTCVRTIMLLHTTYLVNSLCHMWGKQPWKTNDLSTNNWCMSFLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 311
           FGEGWHNNHHAFEYSARMGIEWWQ+D G YVIVFLQAIGVAT+VKLPS++HK+KLAK+ L
Sbjct: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWYVIVFLQAIGVATHVKLPSETHKKKLAKEYL 300

BLAST of HG10002211 vs. ExPASy TrEMBL
Match: A0A6J1ITN3 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479847 PE=3 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.5e-143
Identity = 238/300 (79.33%), Postives = 265/300 (88.33%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDASR+ KRIVKPPMSFGK++  +IDKKVA  +F LH LCIFAPFQ N  A S+C  LF+
Sbjct: 1   MDASRDPKRIVKPPMSFGKKRWTEIDKKVAFTFFILHFLCIFAPFQMNRGALSICFTLFI 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGITISYHRNLSHKSFKIPKWLEY F+YCGVHA QGDPINWVSTHRYHHQFVDTE+
Sbjct: 61  ITGLFGITISYHRNLSHKSFKIPKWLEYSFSYCGVHAFQGDPINWVSTHRYHHQFVDTEQ 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP+ GFWFSYI W L +R+G I EM+ +HRKLDNA DLEKQTFYRFIR+TY+LHPIA
Sbjct: 121 DPHSPIHGFWFSYITWFLYKRNGSIGEMIGRHRKLDNACDLEKQTFYRFIRQTYVLHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGG  +FIWGTCVRTVMLLH T++VNSLCH+WGKQPWKTND S NNW MS LA
Sbjct: 181 LALILYGIGGFSYFIWGTCVRTVMLLHTTYLVNSLCHMWGKQPWKTNDLSTNNWCMSFLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKDKL 311
           FGEGWHNNHHAFEYSARMGIEWWQ+D G YVIVFLQAIGVAT+VKLPS++HK KLAK+ L
Sbjct: 241 FGEGWHNNHHAFEYSARMGIEWWQYDPGWYVIVFLQAIGVATHVKLPSETHKNKLAKEYL 300

BLAST of HG10002211 vs. ExPASy TrEMBL
Match: A0A6J1GV97 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457793 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.8e-143
Identity = 237/298 (79.53%), Postives = 260/298 (87.25%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDASRE K IVKPPMS GKRK  K+DKK+A    FLH LCIFAP Q N+ A S+C  LF+
Sbjct: 1   MDASREPKTIVKPPMSLGKRKWNKMDKKLAFALVFLHFLCIFAPLQINFGALSICFCLFI 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           ITGLFGIT+SYHRNLSHKSFK+PKWLEY FAYCGVHALQGDPI WVSTHRYHHQFVDTE+
Sbjct: 61  ITGLFGITLSYHRNLSHKSFKVPKWLEYVFAYCGVHALQGDPIYWVSTHRYHHQFVDTEQ 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP++GFWFS++IW L + DG   EMV +H KLDNA DLE QTFY+FIRKTY LH I 
Sbjct: 121 DPHSPIKGFWFSHLIWFLHKWDGSASEMVARHEKLDNARDLENQTFYKFIRKTYFLHSIV 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGGIP+FIWGTCVRTVM+LH TFMVNS+CH+WGKQPWKTND SRNNWWMSLLA
Sbjct: 181 LAIILYGIGGIPYFIWGTCVRTVMILHSTFMVNSICHIWGKQPWKTNDLSRNNWWMSLLA 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKD 310
           FGEGWHNNHHAFEYSAR+GIEWWQFD G YVIVFLQAIGVAT+VKLPSQS  QKLAKD
Sbjct: 241 FGEGWHNNHHAFEYSARLGIEWWQFDPGWYVIVFLQAIGVATHVKLPSQSQMQKLAKD 298

BLAST of HG10002211 vs. ExPASy TrEMBL
Match: A0A6J1GWB1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458011 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.7e-140
Identity = 231/298 (77.52%), Postives = 258/298 (86.58%), Query Frame = 0

Query: 12  MDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFM 71
           MDAS+E K IVKPPMSFGK+K  KIDKKVA  +FFLH LCIFAPFQ NW A S+   L++
Sbjct: 1   MDASKEPKSIVKPPMSFGKKKWTKIDKKVAFTFFFLHFLCIFAPFQINWSAISISFTLYI 60

Query: 72  ITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEK 131
           I GLFGITISYHRNLSHKSFKIPKWLEY FAYCGVHA QGDPINWVSTHRYHHQFVDTEK
Sbjct: 61  IIGLFGITISYHRNLSHKSFKIPKWLEYLFAYCGVHAFQGDPINWVSTHRYHHQFVDTEK 120

Query: 132 DPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIA 191
           DPHSP++GFW S+IIW+L + DG + EM+ +HRK DNA DLE QTFYRFI KTY LHPIA
Sbjct: 121 DPHSPIKGFWSSHIIWLLYKGDGSLSEMLGRHRKWDNACDLENQTFYRFIHKTYFLHPIA 180

Query: 192 VAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLA 251
           +A+ILY IGG P+FIWG CVR+V+ LH TFMVNS+CH+WGKQ WKTND SRNNWW+SLL 
Sbjct: 181 LALILYGIGGFPYFIWGMCVRSVIFLHGTFMVNSVCHIWGKQQWKTNDLSRNNWWISLLV 240

Query: 252 FGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLAKD 310
           FGEGWHNNHHAFEYSAR+GIEWWQ+D G YVI FL+AIGVAT+VKLPSQ H QKLA+D
Sbjct: 241 FGEGWHNNHHAFEYSARLGIEWWQYDPGWYVIRFLEAIGVATHVKLPSQRHMQKLAED 298

BLAST of HG10002211 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 381.3 bits (978), Expect = 7.3e-106
Identity = 166/285 (58.25%), Postives = 219/285 (76.84%), Query Frame = 0

Query: 23  KPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISY 82
           K  + + +R+   +D     V   +HLL + APFQ NWRA SV   L+++TGL GIT+S+
Sbjct: 88  KEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGITLSF 147

Query: 83  HRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWF 142
           HRNLSHK+FK+PKWLEY FAYCG  ALQG+PI+WVSTHRYHHQF D+++DPHSP+ GFWF
Sbjct: 148 HRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLDGFWF 207

Query: 143 SYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGI 202
           S++ W+ +        + ++  + +N GDLEKQ FYRF+R TY+LHP+A+AV LY +GG 
Sbjct: 208 SHMNWMFDTN-----TITQRCGEPNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGF 267

Query: 203 PFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHA 262
           PF +WG  VR V + H+T++VNS CH+WGKQ W T D S+NNWW++ LAFGEGWHNNHHA
Sbjct: 268 PFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHA 327

Query: 263 FEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLA 308
           FE+SAR G+EWWQ D+  YV+ FLQAIG+AT+VKLPS++ KQ++A
Sbjct: 328 FEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQKQRMA 367

BLAST of HG10002211 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 340.1 bits (871), Expect = 1.9e-93
Identity = 154/301 (51.16%), Postives = 213/301 (70.76%), Query Frame = 0

Query: 7   QGHKLMDASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVC 66
           + +K M A +      K  M   K KR+ I K  A +  F+H LC+ APF   W A  V 
Sbjct: 10  ENNKKMAADKAEMGRKKRAMWERKWKRLDIVKAFASL--FVHFLCLLAPFNFTWPALRVA 69

Query: 67  LALFMITGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQF 126
           L ++ + GL GIT+SYHRNL+H+SFK+PKWLEYFFAYCG+ A+QGDPI+WVSTHRYHHQF
Sbjct: 70  LIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQF 129

Query: 127 VDTEKDPHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYL 186
            D+++DPHSP +GFWFS+++W+ +   G ++E   K  +  N  DL++Q +Y+F+++T L
Sbjct: 130 TDSDRDPHSPNEGFWFSHLLWLFD--TGYLVE---KCGRRTNVEDLKRQWYYKFLQRTVL 189

Query: 187 LHPIAVAVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWW 246
            H +    +LY  GG+ F  WG  +   M  HVT ++NSLCH+WG + WKTND SRN WW
Sbjct: 190 YHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWW 249

Query: 247 MSLLAFGEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKL 306
           +S+ +FGE WHNNHHAFE SAR G+EWWQ D+  Y++ FL+ IG+AT+VKLPS+S ++++
Sbjct: 250 LSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRM 302

Query: 307 A 308
           A
Sbjct: 310 A 302

BLAST of HG10002211 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 321.6 bits (823), Expect = 6.8e-88
Identity = 141/280 (50.36%), Postives = 196/280 (70.00%), Query Frame = 0

Query: 28  FGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITISYHRNLS 87
           F  R+  ++D       F +H L + APF   W A  V    + I GL GIT+SYHRNL+
Sbjct: 31  FWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVSYHRNLA 90

Query: 88  HKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGFWFSYIIW 147
           H+SFK+PKWLEY  AYC + A+QGDPI+WVSTHRYHHQF D+E+DPHSP +GFWFS+++W
Sbjct: 91  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 150

Query: 148 ILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIGGIPFFIW 207
           I +        +V K  +  N  DL++Q FYRF++KT L H + +   L+ +GG+ F  W
Sbjct: 151 IYDS-----AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTW 210

Query: 208 GTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNHHAFEYSA 267
           G  V   + +HVT ++NSLCH+WG + WKTND SRN WW+S+ +FGE WHNNHHAFE SA
Sbjct: 211 GMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSA 270

Query: 268 RMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLA 308
           R G+EWWQ D+  Y++ F + IG+AT+VK+P+++ ++++A
Sbjct: 271 RQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMA 304

BLAST of HG10002211 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 318.5 bits (815), Expect = 5.8e-87
Identity = 145/281 (51.60%), Postives = 195/281 (69.40%), Query Frame = 0

Query: 21  IVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMITGLFGITI 80
           +V+   SF +R     D     ++   HLL + APF  +W AF V   L  I G+  IT+
Sbjct: 79  VVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITL 138

Query: 81  SYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKDPHSPVQGF 140
           SYHRNLSH+SF +PKWLEY FAY GV A QGDPI WVS HRYHH+  +T++DPHSP QGF
Sbjct: 139 SYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGF 198

Query: 141 WFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAVAVILYVIG 200
           WFS++ WI +     I+E        +N  DL +Q FYRF+++T LLH +A + + Y  G
Sbjct: 199 WFSHMAWIFD--TSSILENCGGE---ENVDDLVRQPFYRFLQRTVLLHMMAYSFLFYFCG 258

Query: 201 GIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAFGEGWHNNH 260
           G+P  +WG  +   + LH+TF+VNS+CH+WG + W T+DFS+NNWW+++L  GEGWHNNH
Sbjct: 259 GMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWHNNH 318

Query: 261 HAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQS 302
           HAFE+SAR G+EWWQ D+   +I FL+AIG+ATNVKLP+++
Sbjct: 319 HAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 353

BLAST of HG10002211 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 311.6 bits (797), Expect = 7.1e-85
Identity = 146/295 (49.49%), Postives = 202/295 (68.47%), Query Frame = 0

Query: 13  DASRETKRIVKPPMSFGKRKRIKIDKKVACVYFFLHLLCIFAPFQSNWRAFSVCLALFMI 72
           D S + K + K   ++  RK  + D   A     +HLLC+ APF   W AF   + L ++
Sbjct: 8   DGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAIL 67

Query: 73  TGLFGITISYHRNLSHKSFKIPKWLEYFFAYCGVHALQGDPINWVSTHRYHHQFVDTEKD 132
           T L  IT SYHRNL+H+SFK+PKWLEY FAY  + ALQGDP++WVS HR+HHQF D+++D
Sbjct: 68  TNLC-ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRD 127

Query: 133 PHSPVQGFWFSYIIWILEERDGRIIEMVRKHRKLDNAGDLEKQTFYRFIRKTYLLHPIAV 192
           PHSP++GFWFS+++WI +    R      K  + +N  DL++Q FYRF++KT +LH +A 
Sbjct: 128 PHSPIEGFWFSHVLWIFDTDYIR-----EKCGRRNNVMDLKQQWFYRFLKKTLVLHILAF 187

Query: 193 AVILYVIGGIPFFIWGTCVRTVMLLHVTFMVNSLCHLWGKQPWKTNDFSRNNWWMSLLAF 252
             ++Y+ GG+P+  W      V+  H T++VNS CH+ G Q W+TND SRN WW++LL  
Sbjct: 188 WTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVWWLALLTM 247

Query: 253 GEGWHNNHHAFEYSARMGIEWWQFDLGLYVIVFLQAIGVATNVKLPSQSHKQKLA 308
           GE WHNNHHAFE SAR G+EW+Q D+  Y+I F QA+G+ATNVKLP+ + K+K+A
Sbjct: 248 GESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQKRKMA 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903351.15.0e-15788.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_038903333.16.3e-15286.90palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_008454220.15.0e-14982.89PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_022955957.11.3e-14479.67palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
XP_023528390.18.3e-14479.67palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [C... [more]
Match NameE-valueIdentityDescription
Q949X01.0e-10458.25Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
P0DOW29.6e-9554.79Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
O657972.6e-9251.16Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW35.8e-9255.56Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Q9SID29.6e-8750.36Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=ADS2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A1S3BY392.4e-14982.89palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GV186.2e-14579.67palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1ITN31.5e-14379.33palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GV975.8e-14379.53palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GWB12.7e-14077.52palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
AT3G15850.17.3e-10658.25fatty acid desaturase 5 [more]
AT1G06080.11.9e-9351.16delta 9 desaturase 1 [more]
AT2G31360.16.8e-8850.3616:0delta9 desaturase 2 [more]
AT3G15870.15.8e-8751.60Fatty acid desaturase family protein [more]
AT1G06100.17.1e-8549.49Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 83..103
score: 36.34
coord: 253..267
score: 60.7
coord: 120..149
score: 48.42
coord: 210..231
score: 41.39
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 31..307
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 59..296
e-value: 1.44194E-72
score: 219.735
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 62..277
e-value: 1.2E-18
score: 67.9
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 31..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002211.1HG10002211.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water