HG10002074 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002074
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionHomogentisate 1,2-dioxygenase
LocationChr11: 3106443 .. 3110038 (-)
RNA-Seq ExpressionHG10002074
SyntenyHG10002074
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTCAATCGGTCGGCGAAACGGACGGCAGAAAATTTCCTTCCGACCTCCCTTACCTGTCCGGCTTCAGCAACCATTTCTCGTCGGAGGCGATTCCTGGCGCTCTTCCTCAATCCCAAAACAGTCCTGTCATATGCCCCTTTGGCCTCTATGCCGAGCAGATCTCCGGCACGTCCTTCACATCGCCTCGGAAAGCTAACTTGTGCAGGTGCTCTATCGTCTTCTCGCTTGATGTGACTTTTTTGCTTTCGTCGTCTTTTTTGTTTACGTATCTCGGTTTTCGCTGCGCAGTTGGCTGTATCGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCAACGCTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCATCAAATTGTACATCGACTCCGACTCAGCTTAGGTGGAGACCGGCGGATGTTCCTGATTCACCGGTGGATTTTGTTGATGGACTGTACACTGTCTGCGGAGCCGGCAGTTCGTTTCTCCGCCATGGGTTTGCTATTCACATGTAAGTATGTAATCAATATTCCTTCTACGGTTTCACTGCTTTTATCGCGGTACTCCCTTTCCAACAATGATTAAATTTCTCGCGGGAAGATGTTATTTGTCGGATTTATTGGTGGGATATTTTGCCAGCGCAGATGGTATGATCCCTCAAGTTTGACTCTGTTTATTTTCTCCATGGGGTTTGTTCCTTACTAACACTAACGAAACAGTACAAATTTCAAACACCGTGTATTTGAATGTTGATACCTCTTTTCGGCATTAGTTTGAGAAGAAAGCAAATAAAGATGCCTTTTACACTGGCCATTGGTTGAATGCTAGGACTGAAACAAAATTGACCATTTCCATAGGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGATGGCGACTTCTTGATAGTTCCTCAGAGTGGAAGTGAGTTATTTTTGCAGGTTACTTTCATTGAAAATTATGAGCACAGTGGTCTGGTTTTCAAGCTGATTCTTTTTTATTTGCAAAATGTAGTTCATTTTAGATTTCTGTGCTGTGTGCTGTAAAGCCGATTCCATTTAGTGAAAGAGATGTAAAGTTGATTCCATATTCATGTTTCCAACTTTTGGCTATATAATCCTATAACTTTCTTTGCATTATCTTGTCATTGAGTTATGAAAGTCTCTACTGTATAATGAAATCTGAACGAAATTATGTCCCTGTCTTATAAGTTGTGAGTTATCAGGATTAGACCCTTATAGAGTATTCTTATTCTGCAGGGCTGTGGATTATTACAGAGTGTGGTAGACTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGATTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGTAGTCATTTCCAGCTTCCTGACCTTGGACCGATAGGTATCTAACTTTTTTTTTTTTTTAATCTTCTTGACCGCAAGCAATTAAGGTTCATGCCATATTCTTTTCCTTTTCCTGGTTTTTTGAGTTTTTCATTTTCGCATAATGTAAATATTGAGGGCTAATTTTATTGACATGTAGGTGCAAATGGTCTTGCTGCACCAAGGGATTTCCTTGCACCTGTAGCCTGGTTTGAAAAGTGTTCTCGTCCCGGTTACACAATTGTTCAAAAATTTGGTGGGGAATTGTTTACTGCGATACAAGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGAAATTATGTTCCCTATAAGGTGGGTATTGCTTATGCTGTGGCAGTCAGGCTCAAATTTATTTTCTTCTTGTGGAATTAAAAATAAGCTTCTGCCTGTGCTTTCTTGCAAGTTTTTTGCTGTTTTGGTCATAAAATAGACTAATGTTACTTATCTTTGCAAGTGAGTGCTAACTCCACCTCAATAAAACTGAAAGATAATCCTTCTCCTTGCAGTATGATCTCAGTAAGTTCTGCCCTTACAATACTGTGTTGTTTGATCACAGTGATCCATCAATAAATACAGGTACATATAGATAATGCATCGTTTCATTTCTCTTTAAATATACTGCTTTTAAGTGTTTTATGTGGTGGTCTGACTTATCATACTACTGTTTTGTTGCAGTATTAACGGCGCCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGTAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGTAACTTATATCATCTCGTTTTAGATTATGAGGCTGGACCTAAATGTTTAGAGGGAAGAAAGAGAAGCTGCTCCTAAATGTTTAGAACAAGAGTCTACAACGCTCAATAATGATTTCTCACTTTTCTCTCAAGGGAGCTCACAAATCACTCTTACAATAGCTTTTCAAACTCTGACAGAGAAAAACCTTTCTATTTTGGTGTTTTTCTTCTTCAACGTAAAAACACCCCCACGGCTTTGTTTTTCTTTTCCAGCCCCTTGCTTTTTTTGGCAACCACCCACAAAAGAAAAACCGCACAGCAGTTTTTTATTTTCAGTTATTGAAGTTATGGGTCTTGCCGTTTTTGGGCTCCAAAATTTTCTTAACACTTGTATGACCTTAAAATCCTCTCTTTGTATAGTTGGAGTCCTTTTAGCTGGACTCCCTTTTATGGGCTGATTTTTTGTATGTTCGCATATTCTTTCATTTTTATCTTAATGAAAGTAGTTGTAAAAAAAATCCTCTTTTTACCCGTTACCTGAATGATAGTGTTTTTAGGCAGAAAATTGGGTGTGGCTTCCCCTATACTGCGATAACTACCTGAAATGGTTCTGATTATACATTTCCAGGGATATACTTTTATTCCAGTGAAGTTTTTTTTTTTTCCCTTGATCTTCAAAATTTTCGATCCATCTTTCAGGCAAAAGCTGATGGGTTCGTTCCCGGAGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACTAAAACTTATGAGGTACGCATTACTTCTTTTGAGTTCCATTGTTCATTTAATTTTCACTATATTCAAGCTTTCTACTATACCAAATAAATAAGATCGATTATTGTTATTATTATCTTTTAGCATATTTTATACATAGTAGTTGGAATAAGTTCGAGAAAAATAGAATGATTAAAGGAAAAATCTTCCTAATAGGTATTTGATCCCATCTCATGCTTCAAACTGTGTATGGTTTTAGTTTAATCAATCCTTGAACAACTCCTCTCATGTTTCGATGAAAAGGTTGAACAGTCTCGTAAGCATTCTATTGATATGCAGGCTACTATTGCTCGAGGGAATGATGTTGGACCATACAAAATCACTGGCACAATGGCGTTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGTTCTTGGGCTCTCGAGTCTCCATTCATGGATCATGACTATTACCAATGCTGGATAGGACTGAAATCTCATTTAAAAAATGAAGCAACTGGAGATACGGATCCACAGGAGGTCAGAACTGAGTCTGAAAATGGAAGACAAATTGAATAA

mRNA sequence

ATGGCTGCTCAATCGGTCGGCGAAACGGACGGCAGAAAATTTCCTTCCGACCTCCCTTACCTGTCCGGCTTCAGCAACCATTTCTCGTCGGAGGCGATTCCTGGCGCTCTTCCTCAATCCCAAAACAGTCCTGTCATATGCCCCTTTGGCCTCTATGCCGAGCAGATCTCCGGCACGTCCTTCACATCGCCTCGGAAAGCTAACTTGTGCAGTTGGCTGTATCGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCAACGCTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCATCAAATTGTACATCGACTCCGACTCAGCTTAGGTGGAGACCGGCGGATGTTCCTGATTCACCGGTGGATTTTGTTGATGGACTGTACACTGTCTGCGGAGCCGGCAGTTCGTTTCTCCGCCATGGGTTTGCTATTCACATGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGATGGCGACTTCTTGATAGTTCCTCAGAGTGGAAGGCTGTGGATTATTACAGAGTGTGGTAGACTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGATTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGTAGTCATTTCCAGCTTCCTGACCTTGGACCGATAGGTGCAAATGGTCTTGCTGCACCAAGGGATTTCCTTGCACCTGTAGCCTGGTTTGAAAAGTGTTCTCGTCCCGGTTACACAATTGTTCAAAAATTTGGTGGGGAATTGTTTACTGCGATACAAGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGAAATTATGTTCCCTATAAGTATGATCTCAGTAAGTTCTGCCCTTACAATACTGTGTTGTTTGATCACAGTGATCCATCAATAAATACAGTATTAACGGCGCCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGTAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGCAAAAGCTGATGGGTTCGTTCCCGGAGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACTAAAACTTATGAGGCTACTATTGCTCGAGGGAATGATGTTGGACCATACAAAATCACTGGCACAATGGCGTTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGTTCTTGGGCTCTCGAGTCTCCATTCATGGATCATGACTATTACCAATGCTGGATAGGACTGAAATCTCATTTAAAAAATGAAGCAACTGGAGATACGGATCCACAGGAGGTCAGAACTGAGTCTGAAAATGGAAGACAAATTGAATAA

Coding sequence (CDS)

ATGGCTGCTCAATCGGTCGGCGAAACGGACGGCAGAAAATTTCCTTCCGACCTCCCTTACCTGTCCGGCTTCAGCAACCATTTCTCGTCGGAGGCGATTCCTGGCGCTCTTCCTCAATCCCAAAACAGTCCTGTCATATGCCCCTTTGGCCTCTATGCCGAGCAGATCTCCGGCACGTCCTTCACATCGCCTCGGAAAGCTAACTTGTGCAGTTGGCTGTATCGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCAACGCTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCATCAAATTGTACATCGACTCCGACTCAGCTTAGGTGGAGACCGGCGGATGTTCCTGATTCACCGGTGGATTTTGTTGATGGACTGTACACTGTCTGCGGAGCCGGCAGTTCGTTTCTCCGCCATGGGTTTGCTATTCACATGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGATGGCGACTTCTTGATAGTTCCTCAGAGTGGAAGGCTGTGGATTATTACAGAGTGTGGTAGACTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGATTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGTAGTCATTTCCAGCTTCCTGACCTTGGACCGATAGGTGCAAATGGTCTTGCTGCACCAAGGGATTTCCTTGCACCTGTAGCCTGGTTTGAAAAGTGTTCTCGTCCCGGTTACACAATTGTTCAAAAATTTGGTGGGGAATTGTTTACTGCGATACAAGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGAAATTATGTTCCCTATAAGTATGATCTCAGTAAGTTCTGCCCTTACAATACTGTGTTGTTTGATCACAGTGATCCATCAATAAATACAGTATTAACGGCGCCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGTAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGCAAAAGCTGATGGGTTCGTTCCCGGAGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACTAAAACTTATGAGGCTACTATTGCTCGAGGGAATGATGTTGGACCATACAAAATCACTGGCACAATGGCGTTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGTTCTTGGGCTCTCGAGTCTCCATTCATGGATCATGACTATTACCAATGCTGGATAGGACTGAAATCTCATTTAAAAAATGAAGCAACTGGAGATACGGATCCACAGGAGGTCAGAACTGAGTCTGAAAATGGAAGACAAATTGAATAA

Protein sequence

MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPDSPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQIE
Homology
BLAST of HG10002074 vs. NCBI nr
Match: XP_038878133.1 (homogentisate 1,2-dioxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.0e-279
Identity = 452/470 (96.17%), Postives = 459/470 (97.66%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           M AQ VGETDGR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 1   MDAQLVGETDGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRK+NLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRWRPADVPD
Sbjct: 61  FTSPRKSNLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWRPADVPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEV PGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLG IGANGLAAPRD
Sbjct: 181 ECGRLEVCPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGLIGANGLAAPRD 240

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFEK S PGYTIVQKFGGELFTAIQDFSPFNVV+WHGNYVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFEKSSSPGYTIVQKFGGELFTAIQDFSPFNVVSWHGNYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGF+PGGASLHNCMTPHGPDTKTYEATIARG DVGPYKITGTMAFMFESSLI 
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGTDVGPYKITGTMAFMFESSLIA 420

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH K EATGDTDPQ+VRTESENGRQI
Sbjct: 421 RVCSWALESPFMDHDYYQCWIGLKSHFKIEATGDTDPQKVRTESENGRQI 470

BLAST of HG10002074 vs. NCBI nr
Match: XP_004137214.1 (homogentisate 1,2-dioxygenase [Cucumis sativus])

HSP 1 Score: 970.7 bits (2508), Expect = 4.6e-279
Identity = 447/470 (95.11%), Postives = 459/470 (97.66%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGETDG  FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG+LWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGKLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGE+VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 181 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYTI+QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 241 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 300

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 420

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPF+DHDYYQCWIGLKSH KNEA GDTDPQ+VR ESENGRQI
Sbjct: 421 RVCSWALESPFIDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIESENGRQI 470

BLAST of HG10002074 vs. NCBI nr
Match: KAA0059007.1 (homogentisate 1,2-dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 965.7 bits (2495), Expect = 1.5e-277
Identity = 444/470 (94.47%), Postives = 458/470 (97.45%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGET+GR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 81

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFG HFQLPDLGPIGANGLAAPRD
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYT++QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH KNEA GDTDPQ+VR +SENGRQI
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQI 491

BLAST of HG10002074 vs. NCBI nr
Match: XP_008451701.1 (PREDICTED: homogentisate 1,2-dioxygenase [Cucumis melo] >TYK19564.1 homogentisate 1,2-dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 963.0 bits (2488), Expect = 9.5e-277
Identity = 443/470 (94.26%), Postives = 457/470 (97.23%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGET+GR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ CPFGLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLNCPFGLYAEQISGTS 81

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFG HFQLPDLGPIGANGLAAPRD
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYT++QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH KNEA GDTDPQ+VR +SENGRQI
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQI 491

BLAST of HG10002074 vs. NCBI nr
Match: KGN53613.2 (hypothetical protein Csa_015034 [Cucumis sativus])

HSP 1 Score: 956.1 bits (2470), Expect = 1.2e-274
Identity = 439/464 (94.61%), Postives = 452/464 (97.41%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGETDG  FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG+LWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGKLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGE+VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 181 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYTI+QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 241 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 300

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 420

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTES 465
           RVCSWALESPF+DHDYYQCWIGLKSH KNEA GDTDPQ+V + S
Sbjct: 421 RVCSWALESPFIDHDYYQCWIGLKSHFKNEAIGDTDPQKVASNS 464

BLAST of HG10002074 vs. ExPASy Swiss-Prot
Match: Q9ZRA2 (Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2)

HSP 1 Score: 788.9 bits (2036), Expect = 3.2e-227
Identity = 362/456 (79.39%), Postives = 397/456 (87.06%), Query Frame = 0

Query: 12  RKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCS 71
           +K   +L Y SGF NHFSSEAI GALP  QNSP++CP+GLYAEQISGTSFTSPRK N  S
Sbjct: 5   KKELEELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRS 64

Query: 72  WLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPDSPVDFVDGLYT 131
           WLYR+KPSVTHEPF+ R+P ++KL+SEF+ASN  + PTQLRWRP D+PDS +DFVDGL+T
Sbjct: 65  WLYRVKPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFT 124

Query: 132 VCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGE 191
           +CGAGSSFLRHGFAIHMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGE
Sbjct: 125 ICGAGSSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGE 184

Query: 192 IVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKC 251
           I V+PQGFRF + LPDG SRGYVAEI+G+HFQLPDLGPIGANGLAA RDFLAP AWFE  
Sbjct: 185 IAVIPQGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDG 244

Query: 252 SRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSIN 311
            RP YTIVQKFGGELFTA QDFSPFNVVAWHGNYVPYKYDL KFCPYNTVL DH DPSIN
Sbjct: 245 LRPEYTIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSIN 304

Query: 312 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFV 371
           TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGF+
Sbjct: 305 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFL 364

Query: 372 PGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPF 431
           PGGASLH+CMTPHGPDT TYEATIAR N + P K+TGTMAFMFES+LIPRVC WALESPF
Sbjct: 365 PGGASLHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPF 424

Query: 432 MDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENG 468
           +DHDYYQCWIGLKSH    +   T+ +   TE E G
Sbjct: 425 LDHDYYQCWIGLKSHFSRISLDKTNVES--TEKEPG 458

BLAST of HG10002074 vs. ExPASy Swiss-Prot
Match: Q5VRH4 (Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE=2 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 9.7e-216
Identity = 342/446 (76.68%), Postives = 386/446 (86.55%), Query Frame = 0

Query: 20  YLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCSWLYRIKPS 79
           YLSG  N  SSEA+ G LP+ QNSP++CP GLYAEQ+SGT FT+PR  NL +WLYRIKPS
Sbjct: 24  YLSGLGNSLSSEAVAGTLPRGQNSPLVCPLGLYAEQLSGTPFTAPRARNLRTWLYRIKPS 83

Query: 80  VTHEPFRQRLPKNEKLISEFN--ASNCTSTPTQLRWRPADVP--DSPVDFVDGLYTVCGA 139
           VTHEPF  R P + +LI +F+   ++  +TPTQLRWRPADVP    P+DF+DGLYTVCGA
Sbjct: 84  VTHEPFHPRRPAHPRLIGDFDRTTTDTVATPTQLRWRPADVPPHHPPLDFIDGLYTVCGA 143

Query: 140 GSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVL 199
           GSSFLRHG+AIHMY ANKSM+ CAFCNADGDFLIVPQ G+L I TECG+L V PGEIVV+
Sbjct: 144 GSSFLRHGYAIHMYAANKSMDGCAFCNADGDFLIVPQQGKLLITTECGKLLVPPGEIVVI 203

Query: 200 PQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKCSRPG 259
           PQGFRF V LPDGPSRGYV+EIFG+HFQLPDLGPIGANGLA+ RDFL+P AWFE+  RPG
Sbjct: 204 PQGFRFAVDLPDGPSRGYVSEIFGTHFQLPDLGPIGANGLASARDFLSPTAWFEQVHRPG 263

Query: 260 YTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLT 319
           YTIVQK+GGELFTA QDFSPFNVVAWHGNYVPYKYDLSKFCP+NTVLFDH+DPS+NTVLT
Sbjct: 264 YTIVQKYGGELFTATQDFSPFNVVAWHGNYVPYKYDLSKFCPFNTVLFDHADPSVNTVLT 323

Query: 320 APTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFVPGGA 379
           APTDKPGVALLDFVIFPPRWLVAE+TFRPPYYHRNCMSEFMGLIYG YEAKADGF+PGGA
Sbjct: 324 APTDKPGVALLDFVIFPPRWLVAENTFRPPYYHRNCMSEFMGLIYGIYEAKADGFLPGGA 383

Query: 380 SLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPFMDHD 439
           SLH+CMTPHGPDTKTYEATI+R +   P +++GT+AFMFES+LIPRVC WAL+SP  D D
Sbjct: 384 SLHSCMTPHGPDTKTYEATISRPDANEPSRLSGTLAFMFESALIPRVCQWALDSPSRDLD 443

Query: 440 YYQCWIGLKSHLKNEATGDTDPQEVR 462
           YYQCWIGLKSH  ++  G T  +  R
Sbjct: 444 YYQCWIGLKSHFSHDNGGATSEEPCR 469

BLAST of HG10002074 vs. ExPASy Swiss-Prot
Match: Q54QI4 (Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 3.9e-148
Identity = 259/434 (59.68%), Postives = 317/434 (73.04%), Query Frame = 0

Query: 17  DLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCSWLYRI 76
           D  Y SGF N F SEAI G LP+ +N+P  CP  LYAEQ+SG +FT+PR     SWLYRI
Sbjct: 7   DYEYQSGFGNSFESEAIKGTLPKGRNAPQNCPLDLYAEQLSGNAFTAPRHTQQRSWLYRI 66

Query: 77  KPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADV-PDSPVDFVDGLYTVCGA 136
           +PSV H P +   P +  L+ + N  N    P QLRW+P  +  D P DFV+GL T+ GA
Sbjct: 67  RPSVCHTPLK---PIDSGLVCDLN--NLHVDPNQLRWKPFPITEDKPHDFVEGLITIAGA 126

Query: 137 GSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVL 196
           G + +RHG AIH+YTA KSMEN +F N+DGDFLIVPQ G L I TE G ++V  GEI V+
Sbjct: 127 GHASVRHGLAIHIYTATKSMENKSFYNSDGDFLIVPQQGTLDIQTEFGFMKVKSGEICVI 186

Query: 197 PQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKCSRPG 256
            +G  F V + +GP+RGY+ E+FGSHF+LPDLGPIGANGLA PRDFL+PVA +EK     
Sbjct: 187 QRGITFSVNV-EGPTRGYICEVFGSHFKLPDLGPIGANGLANPRDFLSPVAAYEKKEGIE 246

Query: 257 YTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLT 316
           +T + KF G+LF+A Q +SPFNVVAWHGNY PYKYDLS FC  N+V FDH DPSI TVLT
Sbjct: 247 HTKINKFLGKLFSATQTYSPFNVVAWHGNYCPYKYDLSLFCVVNSVSFDHLDPSIFTVLT 306

Query: 317 APTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFVPGGA 376
           APT++ GVA  DFVIFPPRWLV E+TFRPPY+HRNCMSEFMGLI G YEAK +GF+PGG 
Sbjct: 307 APTNEVGVAAADFVIFPPRWLVQENTFRPPYFHRNCMSEFMGLIRGVYEAKKEGFLPGGG 366

Query: 377 SLHNCMTPHGPDTKTYEATIARGNDVGPYKITG-TMAFMFESSLIPRVCSWALESPFMDH 436
           SLH+CMTPHGPD+ T+ A I    ++ P KI    +AFMFESSLI  +  +A ++ F+D 
Sbjct: 367 SLHSCMTPHGPDSDTFYAAIKA--ELKPTKIPDVALAFMFESSLILGISDYAKKN-FIDD 426

Query: 437 DYYQCWIGLKSHLK 449
           DY++CW GLK + K
Sbjct: 427 DYWKCWQGLKDNSK 431

BLAST of HG10002074 vs. ExPASy Swiss-Prot
Match: O09173 (Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2)

HSP 1 Score: 510.8 bits (1314), Expect = 1.7e-143
Identity = 249/451 (55.21%), Postives = 312/451 (69.18%), Query Frame = 0

Query: 16  SDLPYLSGFSNHFSSE--AIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCSWL 75
           ++L Y+SGF N  +SE    PG+LP+ QN+P +CP+ LYAEQ+SG++FT PR  N  SWL
Sbjct: 2   AELKYISGFGNECASEDPRCPGSLPKGQNNPQVCPYNLYAEQLSGSAFTCPRNTNKRSWL 61

Query: 76  YRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVP---DSPVDFVDGLY 135
           YRI PSV+H+PF       ++     N       P QLRW+P ++P   +  VDFV GLY
Sbjct: 62  YRILPSVSHKPFE----SIDQGHVTHNWDEVGPDPNQLRWKPFEIPKASEKKVDFVSGLY 121

Query: 136 TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPG 195
           T+CGAG     +G A+H++  N SMEN  F N+DGDFLIVPQ G+L I TE G++ + P 
Sbjct: 122 TLCGAGDIKSNNGLAVHIFLCNSSMENRCFYNSDGDFLIVPQKGKLLIYTEFGKMSLQPN 181

Query: 196 EIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEK 255
           EI V+ +G RF V + +  +RGY+ E++G HF+LPDLGPIGANGLA PRDFL PVAW+E 
Sbjct: 182 EICVIQRGMRFSVDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPVAWYED 241

Query: 256 CSRP-GYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPS 315
              P GYT++ KF G+LF   QD SPFNVVAWHGNY PYKY+L  F   N V FDH+DPS
Sbjct: 242 RRVPGGYTVINKFQGKLFACKQDVSPFNVVAWHGNYTPYKYNLENFMVINAVAFDHADPS 301

Query: 316 INTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADG 375
           I TVLTA + +PGVA+ DFVIFPPRW VA+ TFRPPYYHRNCMSEFMGLI G YEAK  G
Sbjct: 302 IFTVLTAKSLRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIKGHYEAKQGG 361

Query: 376 FVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKIT-GTMAFMFESSLIPRVCSWALE 435
           F+PGG SLH+ MTPHGPD   +E   A    + P +I  GTMAFMFESSL   V  W L+
Sbjct: 362 FLPGGGSLHSAMTPHGPDADCFEK--ASKAKLEPERIADGTMAFMFESSLSLAVTKWGLK 421

Query: 436 S-PFMDHDYYQCWIGLKSHLKNEATGDTDPQ 459
           +   +D +YY+CW  L+SH    +   T+P+
Sbjct: 422 TCSCLDENYYKCWEPLRSHFTPNSRSPTEPK 445

BLAST of HG10002074 vs. ExPASy Swiss-Prot
Match: Q1D8L9 (Homogentisate 1,2-dioxygenase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=hmgA PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 4.9e-143
Identity = 241/425 (56.71%), Postives = 303/425 (71.29%), Query Frame = 0

Query: 20  YLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCSWLYRIKPS 79
           YLSGF N F++EA+PGALP+ QNSP   PFGLYAEQ+SG++FT+PR+ N  SWLYR++PS
Sbjct: 14  YLSGFGNEFATEAVPGALPEGQNSPQRAPFGLYAEQLSGSAFTAPRRENRRSWLYRLRPS 73

Query: 80  VTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPDSPVDFVDGLYTVCGAGSSF 139
             H  F+   P  + L+         ++P +LRW P   P  P DFVDGL T  G G + 
Sbjct: 74  ANHPAFQ---PLAQGLLRSGPFDEVPASPNRLRWSPQPPPAQPTDFVDGLVTYAGNGDAA 133

Query: 140 LRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLPQGF 199
              G +IH+Y AN+SM +  F +ADG+ LIVPQ+GRL ++TE G L+V+PGEI V+P+G 
Sbjct: 134 SGAGISIHLYAANRSMVDRVFFDADGELLIVPQAGRLRLVTELGVLDVAPGEIAVVPRGV 193

Query: 200 RFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKCSRPGYTIV 259
           RF   LP+G + GYV E  G+ F+LPDLGPIGANGLA PRDFL PVA FE   RP   +V
Sbjct: 194 RFRAELPEGQAAGYVCENHGAFFRLPDLGPIGANGLANPRDFLTPVAAFEDVDRP-TEVV 253

Query: 260 QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTD 319
           QKF G L++A   +SP +VVAWHGN VPYKYDL++F   NTV FDH DPSI TVLT+P++
Sbjct: 254 QKFLGRLWSARYSYSPLDVVAWHGNLVPYKYDLARFNTINTVSFDHPDPSIFTVLTSPSE 313

Query: 320 KPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFVPGGASLHN 379
            PG A  DFVIFPPRW+VAEHTFRPP++HRN MSEFMGL++G Y+AKA GF PGG SLHN
Sbjct: 314 VPGTANCDFVIFPPRWMVAEHTFRPPWFHRNVMSEFMGLVHGVYDAKAGGFAPGGGSLHN 373

Query: 380 CMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPFMDHDYYQC 439
           CM+ HGPD  +YE  I    D+ P+KI  T+AFMFES  + R   +A+E+P +  DY  C
Sbjct: 374 CMSGHGPDRTSYEQAIQA--DLKPHKIKDTLAFMFESRWVIRPTRFAMETPALQQDYDAC 432

Query: 440 WIGLK 445
           W G +
Sbjct: 434 WAGFQ 432

BLAST of HG10002074 vs. ExPASy TrEMBL
Match: A0A0A0KYZ6 (Homogentisate 1,2-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=3 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.2e-279
Identity = 447/470 (95.11%), Postives = 459/470 (97.66%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGETDG  FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG+LWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGKLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGE+VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 181 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYTI+QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 241 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 300

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 420

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPF+DHDYYQCWIGLKSH KNEA GDTDPQ+VR ESENGRQI
Sbjct: 421 RVCSWALESPFIDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIESENGRQI 470

BLAST of HG10002074 vs. ExPASy TrEMBL
Match: A0A5A7UWB4 (Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold233G00260 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 7.1e-278
Identity = 444/470 (94.47%), Postives = 458/470 (97.45%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGET+GR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ICPFGLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 81

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFG HFQLPDLGPIGANGLAAPRD
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYT++QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH KNEA GDTDPQ+VR +SENGRQI
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQI 491

BLAST of HG10002074 vs. ExPASy TrEMBL
Match: A0A5D3D7P2 (Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold416G00680 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.6e-277
Identity = 443/470 (94.26%), Postives = 457/470 (97.23%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGET+GR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ CPFGLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLNCPFGLYAEQISGTS 81

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFG HFQLPDLGPIGANGLAAPRD
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYT++QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH KNEA GDTDPQ+VR +SENGRQI
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQI 491

BLAST of HG10002074 vs. ExPASy TrEMBL
Match: A0A1S3BS32 (Homogentisate 1,2-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103492917 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.6e-277
Identity = 443/470 (94.26%), Postives = 457/470 (97.23%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGET+GR FPSDLPYLSGF+NHFSSEAIPGALPQSQNSP+ CPFGLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLNCPFGLYAEQISGTS 81

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNC+STPTQLRW+PAD PD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFG HFQLPDLGPIGANGLAAPRD
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   RPGYT++QKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGFVPGGASLH+CMTPHGPDTKTYEATIARGND GP+KI+GTMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENGRQI 471
           RVCSWALESPFMDHDYYQCWIGLKSH KNEA GDTDPQ+VR +SENGRQI
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQI 491

BLAST of HG10002074 vs. ExPASy TrEMBL
Match: A0A6J1IQY7 (Homogentisate 1,2-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479699 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 7.1e-270
Identity = 434/470 (92.34%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAAQSVGETDGRKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTS 60
           MAAQSVGE DG  FPSDL Y SGF+NHFSSEAIPGALPQ QNSP+ICP+GLYAEQISGTS
Sbjct: 58  MAAQSVGEMDGENFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 117

Query: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPD 120
           FTSPRK N+CSWLYRIKPSVTHEPFR RLPKNEKLISEFNASNC+STPTQLRWRPADVPD
Sbjct: 118 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPADVPD 177

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 178 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 237

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 238 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 297

Query: 241 FLAPVAWFEKCSRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300
           FLAPVAWFE  SRPGYTIVQK+GGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT
Sbjct: 298 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 357

Query: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 358 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 417

Query: 361 GGYEAKADGFVPGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIP 420
           GGYEAKADGF+PGGASLHNCMTPHGPDTKTYEATIARGN+ GPY+IT TMAFMFESSLIP
Sbjct: 418 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 477

Query: 421 RVCSWALESPFMDHDYYQCWIGLKSHLKNE-ATGDTDPQEVRTESENGRQ 470
           RVCSWALESP MDHDYYQCWIGLKSH  +E  T D DP++V+T+ ENGRQ
Sbjct: 478 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPRKVKTDVENGRQ 527

BLAST of HG10002074 vs. TAIR 10
Match: AT5G54080.1 (homogentisate 1,2-dioxygenase )

HSP 1 Score: 788.9 bits (2036), Expect = 2.3e-228
Identity = 362/456 (79.39%), Postives = 397/456 (87.06%), Query Frame = 0

Query: 12  RKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCS 71
           +K   +L Y SGF NHFSSEAI GALP  QNSP++CP+GLYAEQISGTSFTSPRK N  S
Sbjct: 5   KKELEELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRS 64

Query: 72  WLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPDSPVDFVDGLYT 131
           WLYR+KPSVTHEPF+ R+P ++KL+SEF+ASN  + PTQLRWRP D+PDS +DFVDGL+T
Sbjct: 65  WLYRVKPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFT 124

Query: 132 VCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGE 191
           +CGAGSSFLRHGFAIHMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGE
Sbjct: 125 ICGAGSSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGE 184

Query: 192 IVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKC 251
           I V+PQGFRF + LPDG SRGYVAEI+G+HFQLPDLGPIGANGLAA RDFLAP AWFE  
Sbjct: 185 IAVIPQGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDG 244

Query: 252 SRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSIN 311
            RP YTIVQKFGGELFTA QDFSPFNVVAWHGNYVPYKYDL KFCPYNTVL DH DPSIN
Sbjct: 245 LRPEYTIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSIN 304

Query: 312 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFV 371
           TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGF+
Sbjct: 305 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFL 364

Query: 372 PGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPF 431
           PGGASLH+CMTPHGPDT TYEATIAR N + P K+TGTMAFMFES+LIPRVC WALESPF
Sbjct: 365 PGGASLHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPF 424

Query: 432 MDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENG 468
           +DHDYYQCWIGLKSH    +   T+ +   TE E G
Sbjct: 425 LDHDYYQCWIGLKSHFSRISLDKTNVES--TEKEPG 458

BLAST of HG10002074 vs. TAIR 10
Match: AT5G54080.2 (homogentisate 1,2-dioxygenase )

HSP 1 Score: 788.9 bits (2036), Expect = 2.3e-228
Identity = 362/456 (79.39%), Postives = 397/456 (87.06%), Query Frame = 0

Query: 12  RKFPSDLPYLSGFSNHFSSEAIPGALPQSQNSPVICPFGLYAEQISGTSFTSPRKANLCS 71
           +K   +L Y SGF NHFSSEAI GALP  QNSP++CP+GLYAEQISGTSFTSPRK N  S
Sbjct: 5   KKELEELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRS 64

Query: 72  WLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCTSTPTQLRWRPADVPDSPVDFVDGLYT 131
           WLYR+KPSVTHEPF+ R+P ++KL+SEF+ASN  + PTQLRWRP D+PDS +DFVDGL+T
Sbjct: 65  WLYRVKPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFT 124

Query: 132 VCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGE 191
           +CGAGSSFLRHGFAIHMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGE
Sbjct: 125 ICGAGSSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGE 184

Query: 192 IVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEKC 251
           I V+PQGFRF + LPDG SRGYVAEI+G+HFQLPDLGPIGANGLAA RDFLAP AWFE  
Sbjct: 185 IAVIPQGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDG 244

Query: 252 SRPGYTIVQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSIN 311
            RP YTIVQKFGGELFTA QDFSPFNVVAWHGNYVPYKYDL KFCPYNTVL DH DPSIN
Sbjct: 245 LRPEYTIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSIN 304

Query: 312 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFV 371
           TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGF+
Sbjct: 305 TVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFL 364

Query: 372 PGGASLHNCMTPHGPDTKTYEATIARGNDVGPYKITGTMAFMFESSLIPRVCSWALESPF 431
           PGGASLH+CMTPHGPDT TYEATIAR N + P K+TGTMAFMFES+LIPRVC WALESPF
Sbjct: 365 PGGASLHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPF 424

Query: 432 MDHDYYQCWIGLKSHLKNEATGDTDPQEVRTESENG 468
           +DHDYYQCWIGLKSH    +   T+ +   TE E G
Sbjct: 425 LDHDYYQCWIGLKSHFSRISLDKTNVES--TEKEPG 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878133.12.0e-27996.17homogentisate 1,2-dioxygenase isoform X1 [Benincasa hispida][more]
XP_004137214.14.6e-27995.11homogentisate 1,2-dioxygenase [Cucumis sativus][more]
KAA0059007.11.5e-27794.47homogentisate 1,2-dioxygenase [Cucumis melo var. makuwa][more]
XP_008451701.19.5e-27794.26PREDICTED: homogentisate 1,2-dioxygenase [Cucumis melo] >TYK19564.1 homogentisat... [more]
KGN53613.21.2e-27494.61hypothetical protein Csa_015034 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9ZRA23.2e-22779.39Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2[more]
Q5VRH49.7e-21676.68Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE... [more]
Q54QI43.9e-14859.68Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 S... [more]
O091731.7e-14355.21Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2[more]
Q1D8L94.9e-14356.71Homogentisate 1,2-dioxygenase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KYZ62.2e-27995.11Homogentisate 1,2-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=3 SV... [more]
A0A5A7UWB47.1e-27894.47Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A5D3D7P24.6e-27794.26Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BS324.6e-27794.26Homogentisate 1,2-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103492917 PE=3 SV=1[more]
A0A6J1IQY77.1e-27092.34Homogentisate 1,2-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479699 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G54080.12.3e-22879.39homogentisate 1,2-dioxygenase [more]
AT5G54080.22.3e-22879.39homogentisate 1,2-dioxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005708Homogentisate 1,2-dioxygenasePFAMPF04209HgmAcoord: 20..446
e-value: 3.5E-213
score: 708.0
IPR005708Homogentisate 1,2-dioxygenaseTIGRFAMTIGR01015TIGR01015coord: 18..446
e-value: 3.9E-194
score: 643.2
IPR005708Homogentisate 1,2-dioxygenasePANTHERPTHR11056HOMOGENTISATE 1,2-DIOXYGENASEcoord: 14..449
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 280..418
e-value: 1.8E-63
score: 214.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..471
NoneNo IPR availablePANTHERPTHR11056:SF0HOMOGENTISATE 1,2-DIOXYGENASEcoord: 14..449
NoneNo IPR availableCDDcd07000cupin_HGO_Ncoord: 111..219
e-value: 3.14239E-68
score: 211.228
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 17..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002074.1HG10002074.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006559 L-phenylalanine catabolic process
biological_process GO:0006570 tyrosine metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005525 GTP binding
molecular_function GO:0004411 homogentisate 1,2-dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043022 ribosome binding