HG10001952 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10001952 vs. NCBI nr
Match: XP_008453745.1 (PREDICTED: vam6/Vps39-like protein [Cucumis melo] >KAA0044743.1 vam6/Vps39-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1779.6 bits (4608), Expect = 0.0e+00
Identity = 928/998 (92.99%), Postives = 942/998 (94.39%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSPNSS SDRS SSDFH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
           ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                 IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSRGSSGFSDDMESPP 420
           EAMEHFLASQVDITYVLPF PSI+LPKTTL+TETEK +DLDDPHLSRGSSGFSDDMESP 
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVDLDDPHLSRGSSGFSDDMESPV 420

Query: 421 HQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS 480
           HQLLESDENTSLESKK+NHNTLMALIKFLQKKRH+IIEKATAEGTEEVVLDAVGDRYKKS
Sbjct: 421 HQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYKKS 480

Query: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQKNK 540
           YKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQKNK
Sbjct: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQKNK 540

Query: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTDPM 600
           HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL QKFKPEMIIDYLKPLCGTDPM
Sbjct: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTDPM 600

Query: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660
           LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG
Sbjct: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660

Query: 661 NLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS 720
           NLQNEMLQIYLSEVL+WYADLSAQ+KWDEK YSSTRKKLLSALESISGYQPEVLLKRLPS
Sbjct: 661 NLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRLPS 720

Query: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780
           DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT
Sbjct: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780

Query: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAEDTK 840
           LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAEDTK
Sbjct: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAEDTK 840

Query: 841 ISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 900
           +SLSNTDS+RSDGDTDEPGEE +SSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQNL
Sbjct: 841 VSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQNL 900

Query: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKKIG 955
           LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RKPAIKITSDSMCSLCKKKIG
Sbjct: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKPAIKITSDSMCSLCKKKIG 960

BLAST of HG10001952 vs. NCBI nr
Match: XP_038895209.1 (vacuolar sorting protein 39 [Benincasa hispida])

HSP 1 Score: 1776.9 bits (4601), Expect = 0.0e+00
Identity = 930/998 (93.19%), Postives = 937/998 (93.89%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNS GSDRS SSDFHSKS
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSFGSDRSPSSDFHSKS 60

Query: 61  MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
           ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                 IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY
Sbjct: 241 EAPSIVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSRGSSGFSDDMESPP 420
           EAMEHFLASQVDITYVLPF PSI+LPKTTLITETEK MDL DPHLSRGSSGFSDDMESPP
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLITETEKLMDLGDPHLSRGSSGFSDDMESPP 420

Query: 421 HQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS 480
           +QLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS
Sbjct: 421 NQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS 480

Query: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQKNK 540
           YKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQKNK
Sbjct: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQKNK 540

Query: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTDPM 600
           HYSALLELYRCNSMHREALKLLHQLVEESK NESQTE+IQKFKPEMIIDYLKPLCGTDPM
Sbjct: 541 HYSALLELYRCNSMHREALKLLHQLVEESKGNESQTEVIQKFKPEMIIDYLKPLCGTDPM 600

Query: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660
           LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAP LQATYLELMLAMNESSISG
Sbjct: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPTLQATYLELMLAMNESSISG 660

Query: 661 NLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS 720
           NLQNEMLQIYLSEVLDWYADLSAQ KWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS
Sbjct: 661 NLQNEMLQIYLSEVLDWYADLSAQCKWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS 720

Query: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780
           DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYES  NQQPTKSSGNIYLT
Sbjct: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYES--NQQPTKSSGNIYLT 780

Query: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAEDTK 840
           LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKL SG SFKVKGGRAAKKIAAIEGAED K
Sbjct: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLASGSSFKVKGGRAAKKIAAIEGAEDMK 840

Query: 841 ISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 900
           ISL NTDS+RSDGDTDEPGEE +SSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL
Sbjct: 841 ISLGNTDSSRSDGDTDEPGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 900

Query: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKKIG 955
           LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RKPAIKITSDSMCSLCKKKIG
Sbjct: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKPAIKITSDSMCSLCKKKIG 960

BLAST of HG10001952 vs. NCBI nr
Match: XP_022929405.1 (vam6/Vps39-like protein [Cucurbita moschata])

HSP 1 Score: 1728.4 bits (4475), Expect = 0.0e+00
Identity = 905/1000 (90.50%), Postives = 924/1000 (92.40%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
            MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP SSGSDRS +SDFHS S
Sbjct: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61   MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            MELQKEPYVLEKNV+GFSRRSLVSM+VIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61   MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121  ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
            AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121  ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181  EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
            EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181  EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241  ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                  IRSL SPYALIQTIVLRNGRHLIDS HALVVGLDNSAY
Sbjct: 241  EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
            GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361  EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
            EAMEHFLASQVDITYVLPF PSI LPKTTLITETEK MD  LDDPHLS GSSGFSD+MES
Sbjct: 361  EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421  PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
            PPHQLLESD N SLESKK+NHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421  PPHQLLESDGNASLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
            KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQK
Sbjct: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
            NKHYSALLELYRCNSMHREALKLLHQLVEESK +ESQTEL QKFKPEMIIDYLK +CGTD
Sbjct: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
            PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
            SGNLQNEMLQIYLSEVLDWYA+LSAQHKWDEKTYS TRKKLLSALE+ISGY PE+LLKRL
Sbjct: 661  SGNLQNEMLQIYLSEVLDWYANLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721  PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
            P DALSEERAILLGKMNQHELALSLYVHKIH PELALSYCDRVYESVANQQPTKSSGNIY
Sbjct: 721  PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQPTKSSGNIY 780

Query: 781  LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
            LTLLQIYLNPRRTTK FEKRITNLTSPQNMGTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781  LTLLQIYLNPRRTTKYFEKRITNLTSPQNMGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841  TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            TKISLSNT+S RSDGDTDE GEE +SSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841  TKISLSNTESCRSDGDTDEIGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901  NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
            NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RK +IKITSDSMCSLCKKK
Sbjct: 901  NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKSSIKITSDSMCSLCKKK 960

BLAST of HG10001952 vs. NCBI nr
Match: KAG7015348.1 (Vam6/Vps39-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1724.1 bits (4464), Expect = 0.0e+00
Identity = 902/1000 (90.20%), Postives = 922/1000 (92.20%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
            MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP SSGSDRS +SDFHS S
Sbjct: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61   MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            MELQKEPYVLEKNV+GFSRRSLVSM+VIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61   MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121  ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
            AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121  ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181  EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
            EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181  EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241  ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                  IRSL SPYALIQTIVLRNGRHLIDS HALVVGLDNSAY
Sbjct: 241  EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
            GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361  EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
            EAMEHFLASQVDITYVLPF PSI LPKTTLITETEK MD  LDDPHLS GSSGFSD+MES
Sbjct: 361  EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421  PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
            PPHQLLESD N SLESKK+NHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421  PPHQLLESDGNASLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
            KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQK
Sbjct: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
            NKHYSALLELYRCNSMHREALKLLHQLVEESK +ESQTEL QKFKPEMIIDYLK +CGTD
Sbjct: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
            PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
            SGNLQNEMLQIYLS+VLDWYADLSAQHKWDEKTYS TRKKLLSALE+ISGY PE+LLKRL
Sbjct: 661  SGNLQNEMLQIYLSDVLDWYADLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721  PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
            P DALSEERAILLGKMNQHELALSLYVHKIH PELALSYCDRVYESVANQQ TKSSGNIY
Sbjct: 721  PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQSTKSSGNIY 780

Query: 781  LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
            LTLLQIYLNPRRTTK FEKRITNLTSPQNMGTPKLGSGPS KVKG R +KKIAAIEGAED
Sbjct: 781  LTLLQIYLNPRRTTKYFEKRITNLTSPQNMGTPKLGSGPSSKVKGSRTSKKIAAIEGAED 840

Query: 841  TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            TKISLSNT+S+RSDGDTDE GEE +SSIMLDEA+DLLSQRWDRINGAQALKLLP ETKLQ
Sbjct: 841  TKISLSNTESSRSDGDTDEIGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPNETKLQ 900

Query: 901  NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
            NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RK +IKITSDSMCSLCKKK
Sbjct: 901  NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKSSIKITSDSMCSLCKKK 960

BLAST of HG10001952 vs. NCBI nr
Match: XP_022984182.1 (vam6/Vps39-like protein [Cucurbita maxima])

HSP 1 Score: 1723.8 bits (4463), Expect = 0.0e+00
Identity = 903/1000 (90.30%), Postives = 922/1000 (92.20%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
            MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP SSGSDRS +SDFHS S
Sbjct: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61   MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            MELQKEPYVLEKNV+GFSRRSLVSM+VIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61   MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121  ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
            AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121  ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181  EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
            EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181  EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241  ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                  IRSL SPYALIQTIVLRNGRHLIDS HALVVGLDNSAY
Sbjct: 241  EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
            GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361  EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
            EAMEHFLASQVDITYVLPF PSI LPKTTLITETEK MD  LDDPHLS GSSGFSD+MES
Sbjct: 361  EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421  PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
            PPHQLLESD NTSLESKK+NHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421  PPHQLLESDGNTSLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
            KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQK
Sbjct: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
            NKHYSALLELYRCNSMHREALKLLHQLVEESK +ESQTEL QKFKPEMIIDYLK +CGTD
Sbjct: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
            PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
            SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYS TRKKLLSALE+ISGY PE+LLKRL
Sbjct: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721  PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
            P DALSEERAILLGKMNQHELALSLYVHKIH PELALSYCDRVYESVANQQ TKSSGNIY
Sbjct: 721  PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQSTKSSGNIY 780

Query: 781  LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
            LTLLQIYLNPRRTTK FEKRITNLTSPQN GTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781  LTLLQIYLNPRRTTKYFEKRITNLTSPQNTGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841  TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            TKISLSNT+S+RSDGDTDE GEE +SSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841  TKISLSNTESSRSDGDTDETGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901  NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
            NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RK +IKITSDS CSLCKKK
Sbjct: 901  NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKSSIKITSDSTCSLCKKK 960

BLAST of HG10001952 vs. ExPASy Swiss-Prot
Match: Q8L5Y0 (Vacuolar sorting protein 39 OS=Arabidopsis thaliana OX=3702 GN=VPS39 PE=1 SV=1)

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 700/1011 (69.24%), Postives = 818/1011 (80.91%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYS-PNSSGSDRSLSSDFHSK 60
            MVH+AYDSF+LLKD P++I+++ESYGSKLF GC DGSLRIYS P SS SD S        
Sbjct: 1    MVHNAYDSFQLLKDCPARIDAVESYGSKLFAGCYDGSLRIYSPPESSASDPS-------- 60

Query: 61   SMELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAK 120
              EL +E YVLEK V+GFS++ +V+MEV+ SRELLL+LSESIAFH LPNLET+AVITKAK
Sbjct: 61   --ELHQETYVLEKTVAGFSKKPIVAMEVLASRELLLSLSESIAFHGLPNLETVAVITKAK 120

Query: 121  GANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIK 180
            GANAYSWDDRRGFLCF+RQKRVC+F+HDGG GFVEV+++GVPDTVKS+SWCGENICLGIK
Sbjct: 121  GANAYSWDDRRGFLCFSRQKRVCVFKHDGGGGFVEVRDYGVPDTVKSISWCGENICLGIK 180

Query: 181  REYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK--------------------- 240
            +EYVILN  +G L++VFPSGR+APPLV+SLPSGEL+LGK                     
Sbjct: 181  KEYVILNTANGTLSEVFPSGRVAPPLVISLPSGELILGKENIGVFVDQNGKLLQTERICW 240

Query: 241  -----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSA 300
                                   +R LRSPY LIQTIVL+N R L+ S +A++VGLDNS 
Sbjct: 241  SEAPTSIVIQNPYAIALLPRRVEVRLLRSPYPLIQTIVLQNIRRLVKSNNAVIVGLDNSV 300

Query: 301  YGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSY 360
            Y LFPV +GAQIVQLTASGNFEEALALCK+LPP++SSLR+AKESSIH R+AHYLF+NGSY
Sbjct: 301  YVLFPVSIGAQIVQLTASGNFEEALALCKVLPPDESSLRAAKESSIHTRFAHYLFENGSY 360

Query: 361  EEAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDL--DDPHLSRGSSGFSDDME 420
            EEAMEHFLASQVDIT+VL   PSIILPKTT+I + +K +D+  D+  LSRGSSG SDDME
Sbjct: 361  EEAMEHFLASQVDITHVLSMYPSIILPKTTIIPQPDKMVDISGDEASLSRGSSGISDDME 420

Query: 421  -SPPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDR 480
             S P   LES++N  LESKKM+HNTLMALIK+L K+R  +IEKAT+EGTEEV+ DAVG  
Sbjct: 421  SSSPRYFLESEDNADLESKKMSHNTLMALIKYLLKRRPAVIEKATSEGTEEVISDAVGKT 480

Query: 481  Y--------KKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCD 540
            Y        KKS KGRG IP++SGAREMAAILDTALLQALL TGQS AA+ELLKG+NY D
Sbjct: 481  YGANDSSKSKKSSKGRGMIPLNSGAREMAAILDTALLQALLHTGQSGAAIELLKGVNYSD 540

Query: 541  VKICEEILQKNKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMII 600
            VKICEEIL K+K+YSALLEL++ NSMH EALKLL+QL +ESK N+SQT++ Q F PE+II
Sbjct: 541  VKICEEILMKSKNYSALLELFKSNSMHHEALKLLNQLADESKTNQSQTDVTQIFSPELII 600

Query: 601  DYLKPLCGTDPMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLE 660
            +YLKPLC TDPMLVLE+SM VLESCPTQTI+LFLSGNI ADLVNSYLKQHAPN+Q  YLE
Sbjct: 601  EYLKPLCRTDPMLVLEYSMLVLESCPTQTIDLFLSGNISADLVNSYLKQHAPNMQGRYLE 660

Query: 661  LMLAMNESSISGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISG 720
            LM+AMN++++SGNLQNEM+QIYLSEVLD YA  SAQ KWDEK +   RKKLLSALESISG
Sbjct: 661  LMMAMNDTAVSGNLQNEMVQIYLSEVLDLYAAKSAQQKWDEKDHPPERKKLLSALESISG 720

Query: 721  YQPEVLLKRLPSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQ 780
            Y P+ LLKRLP DAL EERA++LGKMNQHELALS+YVHK+H P+LAL+YCDR+YESV   
Sbjct: 721  YSPQPLLKRLPRDALYEERAVILGKMNQHELALSIYVHKLHAPDLALAYCDRIYESVTYL 780

Query: 781  QPTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK-LGSGPSFKVKGGRAA 840
               K S NIYLT+LQIYLNP+++ K+F KRI  L S ++  T K + S  S K KGGR +
Sbjct: 781  PSGKPSSNIYLTVLQIYLNPKKSAKDFAKRIVALGSFESSDTTKMMDSVLSSKAKGGR-S 840

Query: 841  KKIAAIEGAEDTKISL-SNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQ 900
            KKI AIEGAED ++ L S+TDS RSD DT+EP EE  S++M+ E LDLLSQRW+RINGAQ
Sbjct: 841  KKIVAIEGAEDMRVGLSSSTDSGRSDVDTEEPLEEGDSTVMISEVLDLLSQRWERINGAQ 900

Query: 901  ALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKI 954
            ALKLLP+ETKL NLL F+ PLLR SSEA+RN SVIKSLRQSENLQV++ELY +RK   ++
Sbjct: 901  ALKLLPRETKLHNLLPFLAPLLRNSSEAHRNFSVIKSLRQSENLQVKEELYKHRKGVAQV 960

BLAST of HG10001952 vs. ExPASy Swiss-Prot
Match: Q8R5L3 (Vam6/Vps39-like protein OS=Mus musculus OX=10090 GN=Vps39 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.4e-67
Identity = 253/1010 (25.05%), Postives = 444/1010 (43.96%), Query Frame = 0

Query: 2   VHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKSM 61
           +H A++   +L+  P +I+ + ++   L +G   G L +Y          ++S    +S 
Sbjct: 1   MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRKDVVPADVAS---PESG 60

Query: 62  ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSE-SIAFHKLPNLETLAVITKAKG 121
              +    LEK+   FS++ +  + V+   ++L++L E +I  H L   + +  ++KAKG
Sbjct: 61  SCNRFEVTLEKSNKNFSKK-IQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKG 120

Query: 122 ANAYSWD-------DRRGFLCFARQKRVCIFRHDGGRGFVEVK-EFGVPDTVKSMSWCGE 181
           A+ ++ D       +    +C A +K++ ++     R F E++ +F VPD  KSM+WC  
Sbjct: 121 ASLFTCDLQHTETGEEVLRMCVAVRKKLQLYFWK-DREFHELQGDFSVPDVPKSMAWCEN 180

Query: 182 NICLGIKREYVILNAT-SGALTDVFPSGRLAPPLVVSLPSGELLLG-------------- 241
           +IC+G KR+Y ++     G++ ++FP+G+   PLV  L  G++ +G              
Sbjct: 181 SICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGIC 240

Query: 242 ------------------------------KIRSLRSPYALIQTIVLRNGRHLID-SKHA 301
                                         +IR+L  P  L+Q+I L+  R +     + 
Sbjct: 241 TQKCALNWTDIPVAMEHQPPYIVAVLPRYVEIRTL-EPRLLVQSIELQRPRFITSGGSNI 300

Query: 302 LVVGLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIH---I 361
           + V  ++  + L PVP+  QI QL     FE AL L ++    DS     K+  IH    
Sbjct: 301 IYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSE----KQQQIHHIKN 360

Query: 362 RYAHYLFDNGSYEEAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSR 421
            YA  LF    ++E+M+ F     D T+V+   P +      L T+  K +   +P    
Sbjct: 361 LYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDL------LPTDYRKQLQYPNP---- 420

Query: 422 GSSGFSDDMESPPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEE 481
                           L +     LE         +ALI +L +KR  +++K        
Sbjct: 421 ----------------LPTLSGAELEKAH------LALIDYLTQKRSQLVKKLND----- 480

Query: 482 VVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYC 541
                  D    +       P     +++  I+DT LL+  L T  +  A  L    N+C
Sbjct: 481 ------SDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENNHC 540

Query: 542 DVKICEEILQKNKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMI 601
            ++  E +L+K   YS L+ LY    +H +AL++   LV++SK   S  +       E  
Sbjct: 541 HIEESEHVLKKAHKYSELIILYEKKGLHEKALQV---LVDQSKKANSPLK-----GHERT 600

Query: 602 IDYLKPLCGTDPMLVLEFSMTVLESCPTQTIELFLSG-----NIPADLVNSYLKQHAPNL 661
           + YL+ L   +  L+  +S+ VL   P   +++F        ++P D V ++L ++   L
Sbjct: 601 VQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLNFLIENFKAL 660

Query: 662 QATYLELMLAMNESSISGNLQNEMLQIYLSEVLDWYAD-----------LSAQHKWDEKT 721
              YLE ++ + E +      N ++Q+Y  +V     D           + A  +  E  
Sbjct: 661 AIPYLEHIIHVWEET-GSQFHNCLIQLYCEKVQSLMKDYLLSLPTGKSPVPAGEEGGE-- 720

Query: 722 YSSTRKKLLSALESISGYQPEVLLKRLPSDALSEERAILLGKMNQHELALSLYVHKIHVP 781
               R+KLL  LE  S Y P  L+   P D L EERA+LLG+M +HE AL +YVH +   
Sbjct: 721 LGEYRQKLLMFLEISSHYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHVLKDT 780

Query: 782 ELALSYCDRVYESVANQQPTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTP 841
           ++A  YC + Y+     Q  + + ++YL+LL++YL+P                       
Sbjct: 781 KMAKEYCHKHYD-----QNKEGNKDVYLSLLRMYLSP----------------------- 840

Query: 842 KLGSGPSFKVKGGRAAKKIAAIEGAEDTKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEA 901
                PS    G                K+ L    +N                  L  A
Sbjct: 841 -----PSIHCLG--------------PIKLELLEPQAN------------------LQAA 880

Query: 902 LDLLSQRWDRINGAQALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQ 938
           L +L   + +++  +A+ LLP  T++ ++  F+  +L ++++  R + V+K+L  +E L+
Sbjct: 901 LQVLELHYSKLDTTKAINLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLR 880

BLAST of HG10001952 vs. ExPASy Swiss-Prot
Match: Q96JC1 (Vam6/Vps39-like protein OS=Homo sapiens OX=9606 GN=VPS39 PE=1 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 7.5e-66
Identity = 254/1008 (25.20%), Postives = 441/1008 (43.75%), Query Frame = 0

Query: 2   VHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKSM 61
           +H A++   +L+  P +I+ + ++   L +G   G L +Y          ++S    +S 
Sbjct: 1   MHDAFEPVPILEKLPLQIDCLAAWEEWLLVGTKQGHLLLYRIRKDVVPADVAS---PESG 60

Query: 62  ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSE-SIAFHKLPNLETLAVITKAKG 121
              +    LEK+   FS++ +  + V+   ++L++L E +I  H L   + +  ++KAKG
Sbjct: 61  SCNRFEVTLEKSNKNFSKK-IQQIHVVSQFKILVSLLENNIYVHDLLTFQQITTVSKAKG 120

Query: 122 ANAYSWD-------DRRGFLCFARQKRVCIFRHDGGRGFVEVK-EFGVPDTVKSMSWCGE 181
           A+ ++ D       +    +C A +K++ ++     R F E++ +F VPD  KSM+WC  
Sbjct: 121 ASLFTCDLQHTETGEEVLRMCVAVKKKLQLYFWK-DREFHELQGDFSVPDVPKSMAWCEN 180

Query: 182 NICLGIKREYVILNAT-SGALTDVFPSGRLAPPLVVSLPSGELLLG-------------- 241
           +IC+G KR+Y ++     G++ ++FP+G+   PLV  L  G++ +G              
Sbjct: 181 SICVGFKRDYYLIRVDGKGSIKELFPTGKQLEPLVAPLADGKVAVGQDDLTVVLNEEGIC 240

Query: 242 ------------------------------KIRSLRSPYALIQTIVLRNGRHLID-SKHA 301
                                         +IR+   P  L+Q+I L+  R +     + 
Sbjct: 241 TQKCALNWTDIPVAMEHQPPYIIAVLPRYVEIRTF-EPRLLVQSIELQRPRFITSGGSNI 300

Query: 302 LVVGLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIH---I 361
           + V  ++  + L PVP+  QI QL     FE AL L ++    DS     K+  IH    
Sbjct: 301 IYVASNHFVWRLIPVPMATQIQQLLQDKQFELALQLAEMKDDSDSE----KQQQIHHIKN 360

Query: 362 RYAHYLFDNGSYEEAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSR 421
            YA  LF    ++E+M+ F     D T+V+   P +      L T+  K +   +P    
Sbjct: 361 LYAFNLFCQKRFDESMQVFAKLGTDPTHVMGLYPDL------LPTDYRKQLQYPNP---- 420

Query: 422 GSSGFSDDMESPPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEE 481
                           L       LE         +ALI +L +KR  +++K        
Sbjct: 421 ----------------LPVLSGAELEKAH------LALIDYLTQKRSQLVKKLND----- 480

Query: 482 VVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYC 541
                  D    +       P     +++  I+DT LL+  L T  +  A  L    N+C
Sbjct: 481 ------SDHQSSTSPLMEGTPTIKSKKKLLQIIDTTLLKCYLHTNVALVAPLLRLENNHC 540

Query: 542 DVKICEEILQKNKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMI 601
            ++  E +L+K   YS L+ LY    +H +AL++   LV++SK   S  +       E  
Sbjct: 541 HIEESEHVLKKAHKYSELIILYEKKGLHEKALQV---LVDQSKKANSPLK-----GHERT 600

Query: 602 IDYLKPLCGTDPMLVLEFSMTVLESCPTQTIELFLSG-----NIPADLVNSYLKQHAPNL 661
           + YL+ L   +  L+  +S+ VL   P   +++F        ++P D V  +L ++   L
Sbjct: 601 VQYLQHLGTENLHLIFSYSVWVLRDFPEDGLKIFTEDLPEVESLPRDRVLGFLIENFKGL 660

Query: 662 QATYLELMLAMNESSISGNLQNEMLQIYLSEV--------LDWYADLSAQHKWDEK-TYS 721
              YLE ++ + E +      N ++Q+Y  +V        L + A  +     +E+    
Sbjct: 661 AIPYLEHIIHVWEET-GSRFHNCLIQLYCEKVQGLMKEYLLSFPAGKTPVPAGEEEGELG 720

Query: 722 STRKKLLSALESISGYQPEVLLKRLPSDALSEERAILLGKMNQHELALSLYVHKIHVPEL 781
             R+KLL  LE  S Y P  L+   P D L EERA+LLG+M +HE AL +YVH +    +
Sbjct: 721 EYRQKLLMFLEISSYYDPGRLICDFPFDGLLEERALLLGRMGKHEQALFIYVHILKDTRM 780

Query: 782 ALSYCDRVYESVANQQPTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKL 841
           A  YC + Y+   N+   K   ++YL+LL++YL+P                         
Sbjct: 781 AEEYCHKHYD--RNKDGNK---DVYLSLLRMYLSP------------------------- 840

Query: 842 GSGPSFKVKGGRAAKKIAAIEGAEDTKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALD 901
              PS    G                K+ L    +N                  L  AL 
Sbjct: 841 ---PSIHCLG--------------PIKLELLEPKAN------------------LQAALQ 880

Query: 902 LLSQRWDRINGAQALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVR 938
           +L     +++  +AL LLP  T++ ++  F+  +L ++++  R + V+K+L  +E L+V+
Sbjct: 901 VLELHHSKLDTTKALNLLPANTQINDIRIFLEKVLEENAQKKRFNQVLKNLLHAEFLRVQ 880

BLAST of HG10001952 vs. ExPASy Swiss-Prot
Match: A7MB11 (Transforming growth factor-beta receptor-associated protein 1 OS=Bos taurus OX=9913 GN=TGFBRAP1 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 5.8e-26
Identity = 223/1004 (22.21%), Postives = 383/1004 (38.15%), Query Frame = 0

Query: 5   AYDSFELLKD-NPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKSMEL 64
           A +  +LL D +   +E +E  G  L++G SD     +  +    +++L     + +   
Sbjct: 12  AIEREQLLSDRDRGLLECVECCGRNLYVGTSD----CFVYHFLLEEKTLPGGSATFTATR 71

Query: 65  QKEPYV-LEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKGAN 124
           Q   ++  +K VS     S +S        LL+     I+   + +LE +    + KGA 
Sbjct: 72  QLHRHLGFKKAVSELRAASALS-------RLLVLCDGCISLVHMLSLEPVPSGARIKGAT 131

Query: 125 AYSWDDR---------RGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGEN 184
           A++ ++             +   +++ + +F     R  + V+E   P+   +++  G  
Sbjct: 132 AFALNENPVSGDPFCVEVCIISVKRRTIQVFLVYEDRVQI-VREVSTPEQPLAVAVDGHF 191

Query: 185 ICLGIKREYVILNATSGALTDVFP-SGRLAPPLVVSLPSGELL------LGKIRSLRS-- 244
           +CL +  +Y+ILN ++GA  D+FP       P+V  +   E L      LG   ++    
Sbjct: 192 LCLALTTQYIILNYSTGAAQDLFPFCSEERRPIVKRIGRQEFLLAGPGGLGMFATVAGIS 251

Query: 245 ------------------PYALI-----------------QTIVLRNGRHLIDSKHALVV 304
                             PY +                  QT+  + G  L D +  ++V
Sbjct: 252 QRAPVRWSENVIGAAVCFPYVVALDDEFITVHSMLDQQQKQTLPFKEGHILQDFEGRVIV 311

Query: 305 GLDNSAYGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYL 364
                 Y L P+PL  +I  L AS   EEAL L K       + R+  +    + Y   L
Sbjct: 312 ATSKGVYILVPLPLEKRIQDLLASHRVEEALVLAK------GARRNIPKEKFQVMYRRIL 371

Query: 365 FDNG-------SYEEAMEHFLASQVDITYVLPFCPSIILPKTTLITET----EKFMDLDD 424
              G        + +A E F + Q+D+  ++   P ++LP ++  T +     +F DL  
Sbjct: 372 LQAGFIQFAQLQFLKAKELFRSGQLDVRELISLYP-LLLPTSSSFTRSHPPLHEFADL-- 431

Query: 425 PHLSRGSSGFSDDMESPPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATA 484
                             +QL + D++   + K+        L+ +L + R         
Sbjct: 432 ------------------NQLTQGDQDKVAKCKRF-------LMSYLNEVR--------- 491

Query: 485 EGTEEVVLDAVGDRYKKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLK 544
             TE      V + YK+                    +DTALL+ L       + L+LL 
Sbjct: 492 -STE------VANGYKED-------------------IDTALLK-LYAEADHDSLLDLLV 551

Query: 545 GLNYCDVKICEEILQKNKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKF 604
             N+C +      L+K+K Y AL  LY  N     A++L   +V     + ++++L    
Sbjct: 552 TENFCLLPDSAAWLEKHKKYFALGLLYHYNHQDAAAVQLWVSIVNGDIQDSTRSDLY--- 611

Query: 605 KPEMIIDYLKPLCGTDPMLVLEFSMTVLESCPTQTIELFL--------SGNIPADLVNSY 664
             E I+D+L     TDP LV   +  VL+      +++F         SG  P D++ S 
Sbjct: 612 --EYIVDFL--TYSTDPDLVWRHADWVLQRSQEVGVQVFTKRPLDEQQSGFNPDDII-SC 671

Query: 665 LKQHAPNLQATYLELMLAMNESSISGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSS 724
           LK++ P     YLE ++              +  +YL EVL        +    +   + 
Sbjct: 672 LKKY-PQALVKYLEHLVTERRLQ-KEEYHTHLAVLYLDEVLQQRPCTPDK----DAEVTE 731

Query: 725 TRKKLLSALESISGYQPEVLLKRLPSDALSEERAILLGKMNQHELALSLYVHKIHVPELA 784
           T+ KL   L+    Y+   L+ R     L  E AIL GK+ QHE AL + VH++     A
Sbjct: 732 TQAKLRRLLQESDLYRVHFLMDRTRGAGLPLESAILHGKLEQHEEALHILVHELADFPAA 791

Query: 785 LSYCDRVYESVANQQPTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLG 844
             YC  ++ S     P +    ++  LL +YL P                          
Sbjct: 792 EDYC--LWRSEGRDPPYRQ--RLFHLLLAVYLGP-------------------------- 842

Query: 845 SGPSFKVKGGRAAKKIAAIEGAEDTKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDL 904
            GP+        A+ +AA+                                       DL
Sbjct: 852 -GPA------APARTVAAV---------------------------------------DL 842

Query: 905 LSQRWDRINGAQALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRD 935
           L++     + AQ L+LLP    +Q L  F+   +R S  A R + V   L +SENL  + 
Sbjct: 912 LNRHAVEFDAAQVLQLLPGTWSVQLLRPFLMGAMRDSIHARRTTQVAVGLARSENLIYKY 842

BLAST of HG10001952 vs. ExPASy Swiss-Prot
Match: Q1ZXS5 (Vacuolar protein sorting-associated protein 39 homolog OS=Caenorhabditis elegans OX=6239 GN=vps-39 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-22
Identity = 118/503 (23.46%), Postives = 212/503 (42.15%), Query Frame = 0

Query: 455 ILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQKNKHYSALLELYRCNSMHREA 514
           ++DT LL+  + T        +    N C  +  ++IL+      +L  LY     H  A
Sbjct: 470 VVDTTLLKCYIKTKPMLVDSLIRLQSNACTFEDAKKILESEGRLRSLFILYETRKKHEMA 529

Query: 515 LKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTDPMLVLEFSMTVLESCPTQTI 574
           L L         A+    + IQ+     I++YL+ L  ++  L+L+++  VL       +
Sbjct: 530 LDLFIDQSSRPDADPFFDDAIQQ-----IVEYLQSLGNSNLPLILKYAKWVLAKNLEAGV 589

Query: 575 ELFLSG------NIPADLVNSYLKQHAPNLQATYLELMLAMNESSISGNLQNEMLQIYLS 634
           ++F S       N+    V  +LK   P+    YLE ++   E   S      +L+ Y++
Sbjct: 590 QIFTSDETEMARNLNRKAVVEFLKSECPDALIPYLEHVIFKWEEP-SSYFHETLLEFYVA 649

Query: 635 EV-----------LDWYADLSAQHKWDEK-TYSSTRKKLLSALESISGYQPEVLLKRLPS 694
            V            D ++D +     DE       RK+LL  LE    Y P+ +L +L  
Sbjct: 650 RVNTLFKDYVHAFPDAFSDENITRAGDEDGELGLYRKRLLKFLEVSHSYSPQTVLLQLAP 709

Query: 695 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 754
            A  EERA++LG++ QHE AL++YV+ +     A  YC R+Y +  ++    ++  +YL 
Sbjct: 710 HAFYEERALILGRLKQHEQALAIYVNTLKNVPAAEEYC-RLYYNAHDE----TNSQVYLM 769

Query: 755 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAEDTK 814
           L +  ++P +   +        + P +  +   GS                  +   +  
Sbjct: 770 LFRTLVHPNQQQLH--------SIPYHADSTPFGS----------------YRDDVSEAS 829

Query: 815 ISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 874
             +++T S + D +T               A+ +L++  D+I+   AL +LP  T L+ +
Sbjct: 830 TLVNSTSSYQPDVNT---------------AIKILAKHADKIDTVGALNMLPATTPLRVV 889

Query: 875 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKKIG 934
              I  +++ +        + KS+ Q    +  +     +   I +   S C +C+KKI 
Sbjct: 890 FSAINAVIQTTGRQASTRKMEKSVSQCAMSKKLERKNKAQSTKIIVNFSSECVVCEKKIA 921

Query: 935 TSVFAVYPNGKTLVHFVCFRDSQ 940
            S F  YP+G+ L H  C  DSQ
Sbjct: 950 VSAFVRYPDGR-LAHLYCHNDSQ 921

BLAST of HG10001952 vs. ExPASy TrEMBL
Match: A0A5A7TMM2 (Vam6/Vps39-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00280 PE=4 SV=1)

HSP 1 Score: 1779.6 bits (4608), Expect = 0.0e+00
Identity = 928/998 (92.99%), Postives = 942/998 (94.39%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSPNSS SDRS SSDFH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
           ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                 IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSRGSSGFSDDMESPP 420
           EAMEHFLASQVDITYVLPF PSI+LPKTTL+TETEK +DLDDPHLSRGSSGFSDDMESP 
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVDLDDPHLSRGSSGFSDDMESPV 420

Query: 421 HQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS 480
           HQLLESDENTSLESKK+NHNTLMALIKFLQKKRH+IIEKATAEGTEEVVLDAVGDRYKKS
Sbjct: 421 HQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYKKS 480

Query: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQKNK 540
           YKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQKNK
Sbjct: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQKNK 540

Query: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTDPM 600
           HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL QKFKPEMIIDYLKPLCGTDPM
Sbjct: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTDPM 600

Query: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660
           LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG
Sbjct: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660

Query: 661 NLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS 720
           NLQNEMLQIYLSEVL+WYADLSAQ+KWDEK YSSTRKKLLSALESISGYQPEVLLKRLPS
Sbjct: 661 NLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRLPS 720

Query: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780
           DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT
Sbjct: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780

Query: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAEDTK 840
           LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAEDTK
Sbjct: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAEDTK 840

Query: 841 ISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 900
           +SLSNTDS+RSDGDTDEPGEE +SSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQNL
Sbjct: 841 VSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQNL 900

Query: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKKIG 955
           LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RKPAIKITSDSMCSLCKKKIG
Sbjct: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKPAIKITSDSMCSLCKKKIG 960

BLAST of HG10001952 vs. ExPASy TrEMBL
Match: A0A1S3BX35 (vam6/Vps39-like protein OS=Cucumis melo OX=3656 GN=LOC103494389 PE=4 SV=1)

HSP 1 Score: 1779.6 bits (4608), Expect = 0.0e+00
Identity = 928/998 (92.99%), Postives = 942/998 (94.39%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
           MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSPNSS SDRS SSDFH +S
Sbjct: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPNSSASDRSSSSDFHPRS 60

Query: 61  MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            ELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  TELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
           ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
           EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                 IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY
Sbjct: 241 EAPSVVVIQNPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDLDDPHLSRGSSGFSDDMESPP 420
           EAMEHFLASQVDITYVLPF PSI+LPKTTL+TETEK +DLDDPHLSRGSSGFSDDMESP 
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLVDLDDPHLSRGSSGFSDDMESPV 420

Query: 421 HQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYKKS 480
           HQLLESDENTSLESKK+NHNTLMALIKFLQKKRH+IIEKATAEGTEEVVLDAVGDRYKKS
Sbjct: 421 HQLLESDENTSLESKKVNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYKKS 480

Query: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQKNK 540
           YKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQKNK
Sbjct: 481 YKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQKNK 540

Query: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTDPM 600
           HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL QKFKPEMIIDYLKPLCGTDPM
Sbjct: 541 HYSALLELYRCNSMHREALKLLHQLVEESKANESQTEL-QKFKPEMIIDYLKPLCGTDPM 600

Query: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660
           LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG
Sbjct: 601 LVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSISG 660

Query: 661 NLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRLPS 720
           NLQNEMLQIYLSEVL+WYADLSAQ+KWDEK YSSTRKKLLSALESISGYQPEVLLKRLPS
Sbjct: 661 NLQNEMLQIYLSEVLEWYADLSAQNKWDEKIYSSTRKKLLSALESISGYQPEVLLKRLPS 720

Query: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780
           DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT
Sbjct: 721 DALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIYLT 780

Query: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAEDTK 840
           LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGR+AKKIAAIEGAEDTK
Sbjct: 781 LLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRSAKKIAAIEGAEDTK 840

Query: 841 ISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQNL 900
           +SLSNTDS+RSDGDTDEPGEE +SSIMLDEAL+LLSQRWDRINGAQALKLLPKETKLQNL
Sbjct: 841 VSLSNTDSSRSDGDTDEPGEEGSSSIMLDEALNLLSQRWDRINGAQALKLLPKETKLQNL 900

Query: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKKIG 955
           LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RKPAIKITSDSMCSLCKKKIG
Sbjct: 901 LQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKPAIKITSDSMCSLCKKKIG 960

BLAST of HG10001952 vs. ExPASy TrEMBL
Match: A0A6J1EUB2 (vam6/Vps39-like protein OS=Cucurbita moschata OX=3662 GN=LOC111435988 PE=4 SV=1)

HSP 1 Score: 1728.4 bits (4475), Expect = 0.0e+00
Identity = 905/1000 (90.50%), Postives = 924/1000 (92.40%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
            MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP SSGSDRS +SDFHS S
Sbjct: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61   MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            MELQKEPYVLEKNV+GFSRRSLVSM+VIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61   MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121  ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
            AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121  ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181  EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
            EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181  EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241  ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                  IRSL SPYALIQTIVLRNGRHLIDS HALVVGLDNSAY
Sbjct: 241  EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
            GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361  EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
            EAMEHFLASQVDITYVLPF PSI LPKTTLITETEK MD  LDDPHLS GSSGFSD+MES
Sbjct: 361  EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421  PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
            PPHQLLESD N SLESKK+NHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421  PPHQLLESDGNASLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
            KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQK
Sbjct: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
            NKHYSALLELYRCNSMHREALKLLHQLVEESK +ESQTEL QKFKPEMIIDYLK +CGTD
Sbjct: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
            PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
            SGNLQNEMLQIYLSEVLDWYA+LSAQHKWDEKTYS TRKKLLSALE+ISGY PE+LLKRL
Sbjct: 661  SGNLQNEMLQIYLSEVLDWYANLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721  PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
            P DALSEERAILLGKMNQHELALSLYVHKIH PELALSYCDRVYESVANQQPTKSSGNIY
Sbjct: 721  PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQPTKSSGNIY 780

Query: 781  LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
            LTLLQIYLNPRRTTK FEKRITNLTSPQNMGTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781  LTLLQIYLNPRRTTKYFEKRITNLTSPQNMGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841  TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            TKISLSNT+S RSDGDTDE GEE +SSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841  TKISLSNTESCRSDGDTDEIGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901  NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
            NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RK +IKITSDSMCSLCKKK
Sbjct: 901  NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKSSIKITSDSMCSLCKKK 960

BLAST of HG10001952 vs. ExPASy TrEMBL
Match: A0A6J1J1I5 (vam6/Vps39-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482579 PE=4 SV=1)

HSP 1 Score: 1723.8 bits (4463), Expect = 0.0e+00
Identity = 903/1000 (90.30%), Postives = 922/1000 (92.20%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
            MVHSAYDSFELLKDNPSKIESIESYGSKL IGCSDGSLRIYSP SSGSDRS +SDFHS S
Sbjct: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLLIGCSDGSLRIYSPKSSGSDRSPTSDFHSMS 60

Query: 61   MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
            MELQKEPYVLEKNV+GFSRRSLVSM+VIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61   MELQKEPYVLEKNVTGFSRRSLVSMKVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121  ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
            AN YSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGI+R
Sbjct: 121  ANVYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIRR 180

Query: 181  EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
            EYVILNATSGALT+VFPSGRLAPPLVVSLPSGELLLGK                      
Sbjct: 181  EYVILNATSGALTNVFPSGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241  ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                  IRSL SPYALIQTIVLRNGRHLIDS HALVVGLDNSAY
Sbjct: 241  EAPSVVVIQKPYAIALLPRYIEIRSLWSPYALIQTIVLRNGRHLIDSNHALVVGLDNSAY 300

Query: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
            GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAH LFDNGSYE
Sbjct: 301  GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHNLFDNGSYE 360

Query: 361  EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
            EAMEHFLASQVDITYVLPF PSI LPKTTLITETEK MD  LDDPHLS GSSGFSD+MES
Sbjct: 361  EAMEHFLASQVDITYVLPFYPSIALPKTTLITETEKLMDMTLDDPHLSGGSSGFSDEMES 420

Query: 421  PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
            PPHQLLESD NTSLESKK+NHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421  PPHQLLESDGNTSLESKKVNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
            KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS+AALELLKGLNYCDVKICEEILQK
Sbjct: 481  KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSFAALELLKGLNYCDVKICEEILQK 540

Query: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
            NKHYSALLELYRCNSMHREALKLLHQLVEESK +ESQTEL QKFKPEMIIDYLK +CGTD
Sbjct: 541  NKHYSALLELYRCNSMHREALKLLHQLVEESKTSESQTELTQKFKPEMIIDYLKTICGTD 600

Query: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
            PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601  PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
            SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYS TRKKLLSALE+ISGY PE+LLKRL
Sbjct: 661  SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSPTRKKLLSALETISGYHPEILLKRL 720

Query: 721  PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
            P DALSEERAILLGKMNQHELALSLYVHKIH PELALSYCDRVYESVANQQ TKSSGNIY
Sbjct: 721  PPDALSEERAILLGKMNQHELALSLYVHKIHAPELALSYCDRVYESVANQQSTKSSGNIY 780

Query: 781  LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
            LTLLQIYLNPRRTTK FEKRITNLTSPQN GTPKLGSGPS KVKG RA+KKIAAIEGAED
Sbjct: 781  LTLLQIYLNPRRTTKYFEKRITNLTSPQNTGTPKLGSGPSSKVKGSRASKKIAAIEGAED 840

Query: 841  TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            TKISLSNT+S+RSDGDTDE GEE +SSIMLDEA+DLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841  TKISLSNTESSRSDGDTDETGEEGSSSIMLDEAMDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901  NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
            NLLQF+GPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYN RK +IKITSDS CSLCKKK
Sbjct: 901  NLLQFLGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNQRKSSIKITSDSTCSLCKKK 960

BLAST of HG10001952 vs. ExPASy TrEMBL
Match: A0A6J1C5T4 (vam6/Vps39-like protein OS=Momordica charantia OX=3673 GN=LOC111008226 PE=4 SV=1)

HSP 1 Score: 1706.4 bits (4418), Expect = 0.0e+00
Identity = 896/1000 (89.60%), Postives = 914/1000 (91.40%), Query Frame = 0

Query: 1   MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYSPNSSGSDRSLSSDFHSKS 60
           MVHSAYDSFELLKDNPSKIE+I SYGSKLFIGCSDGSLRIYSP SSGSDRS  S+FHSKS
Sbjct: 1   MVHSAYDSFELLKDNPSKIEAIVSYGSKLFIGCSDGSLRIYSPESSGSDRSPPSEFHSKS 60

Query: 61  MELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120
           MELQKEPYVLEKNV+GFSRRSL+SMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG
Sbjct: 61  MELQKEPYVLEKNVTGFSRRSLLSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAKG 120

Query: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIKR 180
           ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPD VKSMSWCGENICLGIKR
Sbjct: 121 ANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDIVKSMSWCGENICLGIKR 180

Query: 181 EYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK---------------------- 240
           EYVILNATSGALTDVFP GRLAPPLVVSLPSGELLLGK                      
Sbjct: 181 EYVILNATSGALTDVFPPGRLAPPLVVSLPSGELLLGKDNIGVFVDQNGKLLQEGRICWS 240

Query: 241 ----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSAY 300
                                 IRSLRSPYALIQTIVLRNGRHLI S HALVVGL NSAY
Sbjct: 241 EAPSVVVIQKPYAVALLPRYIEIRSLRSPYALIQTIVLRNGRHLIHSNHALVVGLGNSAY 300

Query: 301 GLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360
           GLFPVPLGAQIVQ TASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE
Sbjct: 301 GLFPVPLGAQIVQFTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSYE 360

Query: 361 EAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMD--LDDPHLSRGSSGFSDDMES 420
           EAMEHFLASQVDITYVLPF PSI+LPKTTL+TETEK MD  LD PHLSRGSSGFSDDMES
Sbjct: 361 EAMEHFLASQVDITYVLPFYPSIVLPKTTLVTETEKLMDLTLDGPHLSRGSSGFSDDMES 420

Query: 421 PPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDRYK 480
           PPHQL+ESDEN +LESKKMNHNTLMALIKFLQKKRH+IIEKATAEGTEEVVLDAVGDRYK
Sbjct: 421 PPHQLVESDENATLESKKMNHNTLMALIKFLQKKRHSIIEKATAEGTEEVVLDAVGDRYK 480

Query: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCDVKICEEILQK 540
           KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQS AALELLKG NYCDVKICEEILQK
Sbjct: 481 KSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSLAALELLKGPNYCDVKICEEILQK 540

Query: 541 NKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMIIDYLKPLCGTD 600
           N HYSALLELY+CNSMHREALKLLHQLVEESKANES  ELIQKF PEMIIDYLKPLCGTD
Sbjct: 541 NNHYSALLELYKCNSMHREALKLLHQLVEESKANES--ELIQKFNPEMIIDYLKPLCGTD 600

Query: 601 PMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLELMLAMNESSI 660
           PMLVLEFSM VLESCPTQTIELFLSGNIPADLVN YLKQHAPNLQATYLELMLAMNESSI
Sbjct: 601 PMLVLEFSMIVLESCPTQTIELFLSGNIPADLVNFYLKQHAPNLQATYLELMLAMNESSI 660

Query: 661 SGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISGYQPEVLLKRL 720
           SGNLQNEMLQIYLSEVLDW+ADLSAQHKWDEK YSSTRKKLLSAL+SISGY PEVLLKRL
Sbjct: 661 SGNLQNEMLQIYLSEVLDWHADLSAQHKWDEKIYSSTRKKLLSALDSISGYHPEVLLKRL 720

Query: 721 PSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQQPTKSSGNIY 780
           PSDAL EERAILLGKMNQHELALSLYVHKIHV ELALSYCDRVYESVA QQ TKSSGNIY
Sbjct: 721 PSDALYEERAILLGKMNQHELALSLYVHKIHVSELALSYCDRVYESVAYQQSTKSSGNIY 780

Query: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPKLGSGPSFKVKGGRAAKKIAAIEGAED 840
           LTLLQIYLNPRRTTKNFEKRITNLTSPQ MGTPKLGSGP+FKVKGGRAA+KIAAIEGAED
Sbjct: 781 LTLLQIYLNPRRTTKNFEKRITNLTSPQTMGTPKLGSGPTFKVKGGRAARKIAAIEGAED 840

Query: 841 TKISLSNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900
            KIS SNTDS RSDGDTDE GEE +SSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ
Sbjct: 841 MKISHSNTDSGRSDGDTDEMGEEGSSSIMLDEALDLLSQRWDRINGAQALKLLPKETKLQ 900

Query: 901 NLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKITSDSMCSLCKKK 955
           NL QF+GPLLRKSSEAYRNS VIKSLRQSENLQVRDELYN RKP IKIT DSMCSLCKKK
Sbjct: 901 NLRQFLGPLLRKSSEAYRNSLVIKSLRQSENLQVRDELYNQRKPVIKITGDSMCSLCKKK 960

BLAST of HG10001952 vs. TAIR 10
Match: AT4G36630.1 (Vacuolar sorting protein 39 )

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 700/1011 (69.24%), Postives = 818/1011 (80.91%), Query Frame = 0

Query: 1    MVHSAYDSFELLKDNPSKIESIESYGSKLFIGCSDGSLRIYS-PNSSGSDRSLSSDFHSK 60
            MVH+AYDSF+LLKD P++I+++ESYGSKLF GC DGSLRIYS P SS SD S        
Sbjct: 1    MVHNAYDSFQLLKDCPARIDAVESYGSKLFAGCYDGSLRIYSPPESSASDPS-------- 60

Query: 61   SMELQKEPYVLEKNVSGFSRRSLVSMEVIDSRELLLTLSESIAFHKLPNLETLAVITKAK 120
              EL +E YVLEK V+GFS++ +V+MEV+ SRELLL+LSESIAFH LPNLET+AVITKAK
Sbjct: 61   --ELHQETYVLEKTVAGFSKKPIVAMEVLASRELLLSLSESIAFHGLPNLETVAVITKAK 120

Query: 121  GANAYSWDDRRGFLCFARQKRVCIFRHDGGRGFVEVKEFGVPDTVKSMSWCGENICLGIK 180
            GANAYSWDDRRGFLCF+RQKRVC+F+HDGG GFVEV+++GVPDTVKS+SWCGENICLGIK
Sbjct: 121  GANAYSWDDRRGFLCFSRQKRVCVFKHDGGGGFVEVRDYGVPDTVKSISWCGENICLGIK 180

Query: 181  REYVILNATSGALTDVFPSGRLAPPLVVSLPSGELLLGK--------------------- 240
            +EYVILN  +G L++VFPSGR+APPLV+SLPSGEL+LGK                     
Sbjct: 181  KEYVILNTANGTLSEVFPSGRVAPPLVISLPSGELILGKENIGVFVDQNGKLLQTERICW 240

Query: 241  -----------------------IRSLRSPYALIQTIVLRNGRHLIDSKHALVVGLDNSA 300
                                   +R LRSPY LIQTIVL+N R L+ S +A++VGLDNS 
Sbjct: 241  SEAPTSIVIQNPYAIALLPRRVEVRLLRSPYPLIQTIVLQNIRRLVKSNNAVIVGLDNSV 300

Query: 301  YGLFPVPLGAQIVQLTASGNFEEALALCKLLPPEDSSLRSAKESSIHIRYAHYLFDNGSY 360
            Y LFPV +GAQIVQLTASGNFEEALALCK+LPP++SSLR+AKESSIH R+AHYLF+NGSY
Sbjct: 301  YVLFPVSIGAQIVQLTASGNFEEALALCKVLPPDESSLRAAKESSIHTRFAHYLFENGSY 360

Query: 361  EEAMEHFLASQVDITYVLPFCPSIILPKTTLITETEKFMDL--DDPHLSRGSSGFSDDME 420
            EEAMEHFLASQVDIT+VL   PSIILPKTT+I + +K +D+  D+  LSRGSSG SDDME
Sbjct: 361  EEAMEHFLASQVDITHVLSMYPSIILPKTTIIPQPDKMVDISGDEASLSRGSSGISDDME 420

Query: 421  -SPPHQLLESDENTSLESKKMNHNTLMALIKFLQKKRHNIIEKATAEGTEEVVLDAVGDR 480
             S P   LES++N  LESKKM+HNTLMALIK+L K+R  +IEKAT+EGTEEV+ DAVG  
Sbjct: 421  SSSPRYFLESEDNADLESKKMSHNTLMALIKYLLKRRPAVIEKATSEGTEEVISDAVGKT 480

Query: 481  Y--------KKSYKGRGNIPISSGAREMAAILDTALLQALLFTGQSYAALELLKGLNYCD 540
            Y        KKS KGRG IP++SGAREMAAILDTALLQALL TGQS AA+ELLKG+NY D
Sbjct: 481  YGANDSSKSKKSSKGRGMIPLNSGAREMAAILDTALLQALLHTGQSGAAIELLKGVNYSD 540

Query: 541  VKICEEILQKNKHYSALLELYRCNSMHREALKLLHQLVEESKANESQTELIQKFKPEMII 600
            VKICEEIL K+K+YSALLEL++ NSMH EALKLL+QL +ESK N+SQT++ Q F PE+II
Sbjct: 541  VKICEEILMKSKNYSALLELFKSNSMHHEALKLLNQLADESKTNQSQTDVTQIFSPELII 600

Query: 601  DYLKPLCGTDPMLVLEFSMTVLESCPTQTIELFLSGNIPADLVNSYLKQHAPNLQATYLE 660
            +YLKPLC TDPMLVLE+SM VLESCPTQTI+LFLSGNI ADLVNSYLKQHAPN+Q  YLE
Sbjct: 601  EYLKPLCRTDPMLVLEYSMLVLESCPTQTIDLFLSGNISADLVNSYLKQHAPNMQGRYLE 660

Query: 661  LMLAMNESSISGNLQNEMLQIYLSEVLDWYADLSAQHKWDEKTYSSTRKKLLSALESISG 720
            LM+AMN++++SGNLQNEM+QIYLSEVLD YA  SAQ KWDEK +   RKKLLSALESISG
Sbjct: 661  LMMAMNDTAVSGNLQNEMVQIYLSEVLDLYAAKSAQQKWDEKDHPPERKKLLSALESISG 720

Query: 721  YQPEVLLKRLPSDALSEERAILLGKMNQHELALSLYVHKIHVPELALSYCDRVYESVANQ 780
            Y P+ LLKRLP DAL EERA++LGKMNQHELALS+YVHK+H P+LAL+YCDR+YESV   
Sbjct: 721  YSPQPLLKRLPRDALYEERAVILGKMNQHELALSIYVHKLHAPDLALAYCDRIYESVTYL 780

Query: 781  QPTKSSGNIYLTLLQIYLNPRRTTKNFEKRITNLTSPQNMGTPK-LGSGPSFKVKGGRAA 840
               K S NIYLT+LQIYLNP+++ K+F KRI  L S ++  T K + S  S K KGGR +
Sbjct: 781  PSGKPSSNIYLTVLQIYLNPKKSAKDFAKRIVALGSFESSDTTKMMDSVLSSKAKGGR-S 840

Query: 841  KKIAAIEGAEDTKISL-SNTDSNRSDGDTDEPGEEVTSSIMLDEALDLLSQRWDRINGAQ 900
            KKI AIEGAED ++ L S+TDS RSD DT+EP EE  S++M+ E LDLLSQRW+RINGAQ
Sbjct: 841  KKIVAIEGAEDMRVGLSSSTDSGRSDVDTEEPLEEGDSTVMISEVLDLLSQRWERINGAQ 900

Query: 901  ALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQSENLQVRDELYNYRKPAIKI 954
            ALKLLP+ETKL NLL F+ PLLR SSEA+RN SVIKSLRQSENLQV++ELY +RK   ++
Sbjct: 901  ALKLLPRETKLHNLLPFLAPLLRNSSEAHRNFSVIKSLRQSENLQVKEELYKHRKGVAQV 960

BLAST of HG10001952 vs. TAIR 10
Match: AT1G22860.1 (Vacuolar sorting protein 39 )

HSP 1 Score: 48.5 bits (114), Expect = 3.4e-05
Identity = 30/123 (24.39%), Postives = 60/123 (48.78%), Query Frame = 0

Query: 823 MLDEALDLLSQRWDRINGAQALKLLPKETKLQNLLQFIGPLLRKSSEAYRNSSVIKSLRQ 882
           M   A+ LL    + ++  Q L  L  +  L+     I  +LR     +R   ++ ++ +
Sbjct: 822 MFKAAVRLLHNHGESLDPLQVLDKLSPDMPLKLASDTILRMLRARVHHHRQGQIVHNISR 881

Query: 883 SENLQVRDELYNYRKPAIKITSDSMCSLCKKKIGTSVFAVYPNGKTLVHFVCFRDSQNMK 942
           + ++  R      R   ++I  +S+C  C  ++GT +FA+YP+  T+V + C+R     K
Sbjct: 882 ALDVDSRLARLEERSRHMQINDESLCDSCYARLGTKLFAMYPD-DTIVCYKCYRRLGESK 941

Query: 943 AVS 946
           +V+
Sbjct: 942 SVT 943