HG10001899 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001899
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr11: 1508375 .. 1510573 (+)
RNA-Seq ExpressionHG10001899
SyntenyHG10001899
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTCTTTTTGCCTTAGTTTTCATATTTTCTATTGATATTTTAAGTTCATGCACTGTATTGGCTTCATCTGATTTTAATGTTCTTGATTATGGAGCAATTGGAAATGGTAAAACGGACGATTCAAAAGTGAGTAATTTAAGCTACATTGTAATTTTTGTTTATTTAGAGAAATACTTTCGATTGTTTCTTTGATCTCTAATATTATAATAACTTGTTTTCTTTGTATTTTTCTTCATGACAAGGCTTTTCTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGATAATCCAACATTATATGTACCTGATGGAAAAACTTACTTCTTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAAGTCAATTTTGATGTAGTTTATCTTACCTCATTTCTCATATAGAAAATACCCTCCATTTATATTAAATTTATTTAAATAAAACTTGCATTTTTAATAGTCTTACCTTCAACGCCAACGATTTTTTGAAAAGAAAAATATCTACTTTTGCTGGTAAATTTGGGTTTGTGTTGAAAGAAATTAAACTTTCTCCAAACGGTCTAATAACAAAATTAGTTTTTTTTATTATATGATTGGATTGAATATGTTGGAAATTTTTATTGATAATTTTGTTTTTTTAAATATTTATATATATAGCTTAGAGGAACTCTAATGGCTCCAAGCAAGGATGAATGGCCGAGTGACAGTGCAGATAAATGGGTTCAATTTTTTGATATAGAAGGTTTGAATGTCAAAGGAGCTGGACGATTTGATGGTCAAGGCTCTTTTTGGTGGAAAGATTGTGATCATAAACACTGTAAACGACCAACGGTGTGTTTTATATTCCTTTTGAAAATTAGGGTTTGATGGTTTAATTTATAAATGTCAATAATAATTATAATATATATATATATATATATATATATATATATATATATATATATATGATTTTATAGGCATTGTTTTTCCACAATTGCAACGGACTTCAAATGAAAGAAATGAAGCATGTCAACAGTGGCAAGAATCACATCTCTATAAATGTTTGCAACGATGTCATTTTCTCAAACATTCATATTAGTGCTCCCAATGAAAGTCCTAACACGGATGGAATTGATATTTCTAGATCAAAAAATATTCTTATCCAAGATTCTTTTATAGGAACAGGTAAGACATTGGTATTATTATTATTATTTATTGTAGTTGATATAGTTTAATTTATAATTATACATTTTTGTTTCATTTTGTTTCTTATTAACTTCAATGTAAAAATGTAGGTGATGATTGTATCGCCATAAATAATGGATCTTCCAATATCAACATAATTGGTATCACATGTGGCCCTGGTCATGGCATAAGGTAAAAATTACATTGTTTTTCTTTTAACACCCTGGTCATGGCATATATATATTTTTCTCTCCATATTATCCTTTCATTAAAGTTTTATTAATTTTCAAATTTTCATCTCTGACAAAAAAAGCAAAAAAAAGGAATAACCATTATAATTTCTCCTTTTCTTACAAATGTTGTGGTGTTTAATTGAATGATGTAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAATGTGGTTGAAAATGTTCATGTAAGCAACTGCCTCCTACAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGTGTTAAATACTTTTAAAATTTATTTATATATACAATGTCTAATGTACATTATATAAATAGTTATAAATGATTTCTCTTAACTGTAATTTCAGGGTGGATACGGATATGCAAGGAATATCACATTCGAGAAAATAACACTAAAAAATGCCAAAAATCCAATCATTATTGATCAATATTACTCATCTTATGCATATTCAAGAAAAAATAAGGTATATATACAATAAAAATGAAAATTTTTCTTTGTTTTTATTACATATTGTTCATATATATTTATCATATTGATTTTTTTCTTCAGGGAATGGACATAAAAGTGAGTGATGTGACATTTCGTGAAGTTAATGGAACGTCAGCAAATGAAGATGCAATTACTTTGAACTGCTCTCGAGCTCGTTGCACAAATATTATATTGGACGATGTGAGCATAAAAACAACAATTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAAAATGTCGATGGAAAATCTATATATGTTGTCCCAACCGTGCCTTGCTTATCAAAATCCGATTAA

mRNA sequence

ATGAAGCTTCTTTTTGCCTTAGTTTTCATATTTTCTATTGATATTTTAAGTTCATGCACTGTATTGGCTTCATCTGATTTTAATGTTCTTGATTATGGAGCAATTGGAAATGGTAAAACGGACGATTCAAAAGCTTTTCTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGATAATCCAACATTATATGTACCTGATGGAAAAACTTACTTCTTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAACTTAGAGGAACTCTAATGGCTCCAAGCAAGGATGAATGGCCGAGTGACAGTGCAGATAAATGGGTTCAATTTTTTGATATAGAAGGTTTGAATGTCAAAGGAGCTGGACGATTTGATGGTCAAGGCTCTTTTTGGTGGAAAGATTGTGATCATAAACACTGTAAACGACCAACGGTGTGTGATGATTGTATCGCCATAAATAATGGATCTTCCAATATCAACATAATTGGTATCACATGTGGCCCTGGTCATGGCATAAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAATGTGGTTGAAAATGTTCATGTAAGCAACTGCCTCCTACAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGGTGGATACGGATATGCAAGGAATATCACATTCGAGAAAATAACACTAAAAAATGCCAAAAATCCAATCATTATTGATCAATATTACTCATCTTATGCATATTCAAGAAAAAATAAGGGAATGGACATAAAAGTGAGTGATGTGACATTTCGTGAAGTTAATGGAACGTCAGCAAATGAAGATGCAATTACTTTGAACTGCTCTCGAGCTCGTTGCACAAATATTATATTGGACGATGTGAGCATAAAAACAACAATTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAAAATGTCGATGGAAAATCTATATATGTTGTCCCAACCGTGCCTTGCTTATCAAAATCCGATTAA

Coding sequence (CDS)

ATGAAGCTTCTTTTTGCCTTAGTTTTCATATTTTCTATTGATATTTTAAGTTCATGCACTGTATTGGCTTCATCTGATTTTAATGTTCTTGATTATGGAGCAATTGGAAATGGTAAAACGGACGATTCAAAAGCTTTTCTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGATAATCCAACATTATATGTACCTGATGGAAAAACTTACTTCTTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAACAACTTAGAGGAACTCTAATGGCTCCAAGCAAGGATGAATGGCCGAGTGACAGTGCAGATAAATGGGTTCAATTTTTTGATATAGAAGGTTTGAATGTCAAAGGAGCTGGACGATTTGATGGTCAAGGCTCTTTTTGGTGGAAAGATTGTGATCATAAACACTGTAAACGACCAACGGTGTGTGATGATTGTATCGCCATAAATAATGGATCTTCCAATATCAACATAATTGGTATCACATGTGGCCCTGGTCATGGCATAAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAATGTGGTTGAAAATGTTCATGTAAGCAACTGCCTCCTACAAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGGTGGATACGGATATGCAAGGAATATCACATTCGAGAAAATAACACTAAAAAATGCCAAAAATCCAATCATTATTGATCAATATTACTCATCTTATGCATATTCAAGAAAAAATAAGGGAATGGACATAAAAGTGAGTGATGTGACATTTCGTGAAGTTAATGGAACGTCAGCAAATGAAGATGCAATTACTTTGAACTGCTCTCGAGCTCGTTGCACAAATATTATATTGGACGATGTGAGCATAAAAACAACAATTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAAAATGTCGATGGAAAATCTATATATGTTGTCCCAACCGTGCCTTGCTTATCAAAATCCGATTAA

Protein sequence

MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPCKSKQLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWWKDCDHKHCKRPTVCDDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDDVSIKTTIPGKEAKAFCQNVDGKSIYVVPTVPCLSKSD
Homology
BLAST of HG10001899 vs. NCBI nr
Match: XP_038888676.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 553.1 bits (1424), Expect = 1.6e-153
Identity = 281/396 (70.96%), Postives = 299/396 (75.51%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLLFAL+FIF+IDIL+   V ASS+FNVLDYGAIGNGK DDS+AFLKAWNDVCGATED+
Sbjct: 1   MKLLFALIFIFTIDILTLSIVSASSNFNVLDYGAIGNGKADDSEAFLKAWNDVCGATEDS 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY LNPLKFQGPCKS Q    LRGTLMAPSKDEWPSD ADKWVQF+DIEGL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSNQVNFDLRGTLMAPSKDEWPSDGADKWVQFYDIEGL 120

Query: 121 NVKGAGRFDGQGSFWWKDCDHKHCKRPTVC------------------------------ 180
           N+KGAG+FDGQGS WWKDCD KHCKRPT                                
Sbjct: 121 NLKGAGKFDGQGSLWWKDCDRKHCKRPTALFFHNCNGLQVKEMKHINSGRNHISINDCND 180

Query: 181 --------------------------------------DDCIAINNGSSNINIIGITCGP 240
                                                 DDCIAIN GSS+INIIGITCGP
Sbjct: 181 VIFSNIQILAPEESPNTDGIDISKSKNVLIQDSFIATGDDCIAINEGSSDINIIGITCGP 240

Query: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNA 300
           GHGISIGSLGKDG YNVVEN+HVSNCLLQGTQNGVRIKTWQGGYGYA+NITFEKITLKNA
Sbjct: 241 GHGISIGSLGKDGAYNVVENIHVSNCLLQGTQNGVRIKTWQGGYGYAKNITFEKITLKNA 300

Query: 301 KNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDD 325
           KNPIIIDQYY+SYAYSRK KGMDIKVSDVT+REVNGTSANEDAITLNCSRARCTNIILD+
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEDAITLNCSRARCTNIILDN 360

BLAST of HG10001899 vs. NCBI nr
Match: KGN53196.1 (hypothetical protein Csa_014667 [Cucumis sativus])

HSP 1 Score: 545.4 bits (1404), Expect = 3.3e-151
Identity = 273/392 (69.64%), Postives = 298/392 (76.02%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +F++SI ILSS TVL SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATE++
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKG 120
           PTL+VPDGKTY LNPLKFQGPCKSKQL GTLMAP+KDEW SD   +W+QFFDIEGLN++G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 AGRFDGQGSFWWKDCDHKHCKRPT----------------------------VC------ 180
            GRFDGQGS WWKDCDHKHCKRPT                            +C      
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 ----------------------------------DDCIAINNGSSNINIIGITCGPGHGI 240
                                             DDCIAINNGSSNINI GITCGPGHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPI 300
           SIGSLGKDGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKIT+KNAKNPI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 IIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDDVSIK 325
           IIDQYYSSYAYSRK KGMDIKVSDVT+REVNGTSANEDAIT NCS+ARC+NIILD+V+IK
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

BLAST of HG10001899 vs. NCBI nr
Match: XP_031739967.1 (probable polygalacturonase At3g15720 [Cucumis sativus])

HSP 1 Score: 539.7 bits (1389), Expect = 1.8e-149
Identity = 273/396 (68.94%), Postives = 298/396 (75.25%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +F++SI ILSS TVL SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATE++
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY LNPLKFQGPCKSKQ    L GTLMAP+KDEW SD   +W+QFFDIEGL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQVIFYLGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 NVKGAGRFDGQGSFWWKDCDHKHCKRPT----------------------------VC-- 180
           N++G GRFDGQGS WWKDCDHKHCKRPT                            +C  
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 --------------------------------------DDCIAINNGSSNINIIGITCGP 240
                                                 DDCIAINNGSSNINI GITCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKIT+KNA
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDD 325
           KNPIIIDQYYSSYAYSRK KGMDIKVSDVT+REVNGTSANEDAIT NCS+ARC+NIILD+
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

BLAST of HG10001899 vs. NCBI nr
Match: KAA0044700.1 (putative polygalacturonase [Cucumis melo var. makuwa] >TYK16887.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 526.2 bits (1354), Expect = 2.0e-145
Identity = 267/396 (67.42%), Postives = 294/396 (74.24%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +  +SI ILSS TVL+SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGAT+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY L+PLKFQGPCKS Q    L GTLMAP+KDEWP+D   KWVQF D+EGL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 NVKGAGRFDGQGSFWWKDCDHKHCKRPT----------------------------VC-- 180
           NV+G GRFDGQGS WWK CDHK CKRPT                            +C  
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 --------------------------------------DDCIAINNGSSNINIIGITCGP 240
                                                 DDC+AINNGSSNINI  ITCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKITLKN+
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDD 325
           KNPIIIDQYY+SYAYSRK KGMDIKVSDVT+REVNGTSANE+AIT NCS+ARC+NIILD+
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

BLAST of HG10001899 vs. NCBI nr
Match: XP_008454473.1 (PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 261/385 (67.79%), Postives = 289/385 (75.06%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +  +SI ILSS TVL+SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGAT+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY L+PLKFQGPCKS Q    L GTLMAP+KDEWP+D   KWVQF D+EGL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 NVKGAGRFDGQGSFWWK-----DCD----------------------------HKHCKRP 180
           NV+G GRFDGQGS WWK     +C+                            H H   P
Sbjct: 121 NVQGGGRFDGQGSCWWKALFFHNCNGLQMNGMKHVNSAKNHISINLCNDVIFSHLHISAP 180

Query: 181 T------------------------VCDDCIAINNGSSNINIIGITCGPGHGISIGSLGK 240
                                      DDC+AINNGSSNINI  ITCGPGHGISIGSLGK
Sbjct: 181 EDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPGHGISIGSLGK 240

Query: 241 DGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYS 300
           DGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKITLKN+KNPIIIDQYY+
Sbjct: 241 DGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSKNPIIIDQYYT 300

Query: 301 SYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDDVSIKTTIPGKE 325
           SYAYSRK KGMDIKVSDVT+REVNGTSANE+AIT NCS+ARC+NIILD+V+IK T PG+E
Sbjct: 301 SYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNVNIKMTNPGEE 360

BLAST of HG10001899 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 171/389 (43.96%), Postives = 206/389 (52.96%), Query Frame = 0

Query: 11  FSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKT 70
           FS+  L   T  +S+  +V  +GA+G+G TDDS+AFLKAW  VC  T D     VP G T
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG-QFVVPAGMT 69

Query: 71  YFLNPLKFQGPCKSK----QLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDG 130
           + L PLKFQG CKS     Q+ G L+APSK  W  D  D+W+ F DIEGL ++G G  +G
Sbjct: 70  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEING 129

Query: 131 QGSFWW------------KDCD---------------HKH---CKRPTVC---------- 190
           QGS WW            + C+               H H   C   T+           
Sbjct: 130 QGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESS 189

Query: 191 ------------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGE 250
                                   DDCIAIN+G+SNI+I GI CGPGHGISIGSLGKDGE
Sbjct: 190 PNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGE 249

Query: 251 YNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYY---- 310
              VENV V NC  +GT NG RIKTWQGG GYAR ITF  ITL N +NPIIIDQ+Y    
Sbjct: 250 TATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGD 309

Query: 311 SSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCS-RARCTNIILDDVSIKTTI-- 325
           S  A  RK+    ++VS V F    GTS +E  +   CS R  CT I L D+ I+T    
Sbjct: 310 SDNAKDRKSSA--VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSG 369

BLAST of HG10001899 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.5e-53
Identity = 125/373 (33.51%), Postives = 176/373 (47.18%), Query Frame = 0

Query: 28  NVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPCKSK-- 87
           +V ++GA GNG TDD+KAF  AW   C +++    + VP+  T  L P+   GPCK++  
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTAC-SSKVKTRILVPENYTCLLRPIDLSGPCKARLT 113

Query: 88  -QLRGTLMAPS-KDEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWW-KDCDHKH--- 147
            Q+ GT++AP+  D W   +  KW+ F  +  L V+G G  +G G  WW + C H H   
Sbjct: 114 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNP 173

Query: 148 -----------------------------------CKRPTVC------------------ 207
                                              C+R T+                   
Sbjct: 174 CRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHI 233

Query: 208 ----------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVENVH 267
                           DDCI+I   S+ I+I  I CGPGHGISIGSLGK   +  V ++ 
Sbjct: 234 SVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDIT 293

Query: 268 VSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKNKGM 323
           V   ++  T NGVRIKTWQGG G    I F  I + N  NPIIIDQYY        N+  
Sbjct: 294 VDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTS 353

BLAST of HG10001899 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.8e-51
Identity = 132/376 (35.11%), Postives = 176/376 (46.81%), Query Frame = 0

Query: 27  FNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPCK--- 86
           FNV  YGA+G+GK D + AF K WN  C     +    VP  K + +N L F GPC+   
Sbjct: 60  FNVEHYGAVGDGKHDSTDAFEKTWNAACNKL--SAVFLVPANKKFVVNNLVFYGPCQPHF 119

Query: 87  SKQLRGTLMA-PSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWWKD-------- 146
           S ++ GT+ A P   +W +     W+ F  +   N+ G G  DGQG+ WW D        
Sbjct: 120 SFKVDGTIAAYPDPAKWKNSKI--WMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTINGR 179

Query: 147 --CDHKHCKRPTVC---------------------------------------------- 206
             C+ K   RPT                                                
Sbjct: 180 TVCNDK--GRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDSPN 239

Query: 207 ----------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYN 266
                                 DDC+A+  GSSNI I  +TCGPGHG+SIGSLGK    +
Sbjct: 240 TDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNSRS 299

Query: 267 VVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYS 320
            V  VH+       TQNG+RIKTWQGG G A +IT+E + + NA+NPI+I+Q+Y + A +
Sbjct: 300 EVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSAAA 359

BLAST of HG10001899 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 129/370 (34.86%), Postives = 179/370 (48.38%), Query Frame = 0

Query: 23  ASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPC 82
           A++ FNV  YGA+G+GK D ++AF   WN  C   + +  L VP  K +F+N L F+GPC
Sbjct: 55  AATVFNVEQYGAVGDGKHDSTEAFATTWNAAC--KKASAVLLVPANKKFFVNNLVFRGPC 114

Query: 83  K---SKQLRGTLMA-PSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWWKD---- 142
           +   S ++ GT++A P    W +     W+QF  +   N+ G G  DGQG  WW      
Sbjct: 115 QPHLSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV 174

Query: 143 ------CDHKHCKRPTVC------------------------------------------ 202
                 C+ ++  RPT                                            
Sbjct: 175 VNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPR 234

Query: 203 --------------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKD 262
                                     DDCIAI  GSSNI I  + CGPGHGISIGSLG+D
Sbjct: 235 DSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRD 294

Query: 263 GEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSS 310
                V +VHV+      TQNG+RIKTWQGG G A  IT+E + + N++NPI+I+Q+Y +
Sbjct: 295 NSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCT 354

BLAST of HG10001899 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 198.7 bits (504), Expect = 9.8e-50
Identity = 134/360 (37.22%), Postives = 170/360 (47.22%), Query Frame = 0

Query: 28  NVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPCKS--- 87
           +V D+GA G+GKTDD++AF+ AW   C ++     L VP G TY L  ++  GPC S   
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKAC-SSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 128

Query: 88  KQLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKG--AGRFDGQGSFWWKD-CDH---K 147
            Q+ GTL A  K     D   KW+ F  +  L+V G   G  DG G  WW++ C     K
Sbjct: 129 VQIFGTLSASQKRSDYKD-ISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAK 188

Query: 148 HC-KRPTVC--------------------------------------------------- 207
            C K PT                                                     
Sbjct: 189 PCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIH 248

Query: 208 -----------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVENV 267
                            DDCI+I +GS N+ I  ITCGPGHGISIGSLG D     V  V
Sbjct: 249 ITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGV 308

Query: 268 HVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKNKG 309
            V    L GT NGVRIKT+QGG G A NI F+ I + N KNPIIIDQ Y   +     K 
Sbjct: 309 TVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKS 368

BLAST of HG10001899 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.6e-151
Identity = 273/392 (69.64%), Postives = 298/392 (76.02%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +F++SI ILSS TVL SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATE++
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKG 120
           PTL+VPDGKTY LNPLKFQGPCKSKQL GTLMAP+KDEW SD   +W+QFFDIEGLN++G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 121 AGRFDGQGSFWWKDCDHKHCKRPT----------------------------VC------ 180
            GRFDGQGS WWKDCDHKHCKRPT                            +C      
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 181 ----------------------------------DDCIAINNGSSNINIIGITCGPGHGI 240
                                             DDCIAINNGSSNINI GITCGPGHGI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 241 SIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPI 300
           SIGSLGKDGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKIT+KNAKNPI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

Query: 301 IIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDDVSIK 325
           IIDQYYSSYAYSRK KGMDIKVSDVT+REVNGTSANEDAIT NCS+ARC+NIILD+V+IK
Sbjct: 301 IIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIK 360

BLAST of HG10001899 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 9.9e-146
Identity = 267/396 (67.42%), Postives = 294/396 (74.24%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +  +SI ILSS TVL+SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGAT+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY L+PLKFQGPCKS Q    L GTLMAP+KDEWP+D   KWVQF D+EGL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 NVKGAGRFDGQGSFWWKDCDHKHCKRPT----------------------------VC-- 180
           NV+G GRFDGQGS WWK CDHK CKRPT                            +C  
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 --------------------------------------DDCIAINNGSSNINIIGITCGP 240
                                                 DDC+AINNGSSNINI  ITCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKITLKN+
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDD 325
           KNPIIIDQYY+SYAYSRK KGMDIKVSDVT+REVNGTSANE+AIT NCS+ARC+NIILD+
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

BLAST of HG10001899 vs. ExPASy TrEMBL
Match: A0A1S3BYT5 (probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.3e-141
Identity = 261/385 (67.79%), Postives = 289/385 (75.06%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL A +  +SI ILSS TVL+SSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGAT+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL+VPDGKTY L+PLKFQGPCKS Q    L GTLMAP+KDEWP+D   KWVQF D+EGL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 NVKGAGRFDGQGSFWWK-----DCD----------------------------HKHCKRP 180
           NV+G GRFDGQGS WWK     +C+                            H H   P
Sbjct: 121 NVQGGGRFDGQGSCWWKALFFHNCNGLQMNGMKHVNSAKNHISINLCNDVIFSHLHISAP 180

Query: 181 T------------------------VCDDCIAINNGSSNINIIGITCGPGHGISIGSLGK 240
                                      DDC+AINNGSSNINI  ITCGPGHGISIGSLGK
Sbjct: 181 EDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPGHGISIGSLGK 240

Query: 241 DGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYS 300
           DGEYNVVENVHVSNCLL+ TQNG+RIKTW+GGYGYA+NITFEKITLKN+KNPIIIDQYY+
Sbjct: 241 DGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSKNPIIIDQYYT 300

Query: 301 SYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILDDVSIKTTIPGKE 325
           SYAYSRK KGMDIKVSDVT+REVNGTSANE+AIT NCS+ARC+NIILD+V+IK T PG+E
Sbjct: 301 SYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNVNIKMTNPGEE 360

BLAST of HG10001899 vs. ExPASy TrEMBL
Match: A0A6J1GVS6 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111457994 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.1e-131
Identity = 244/397 (61.46%), Postives = 282/397 (71.03%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKL  + VF+F I +LSS T  ASS FNVLDYGA GNGKTDDS+AFLKAWND C +TEDN
Sbjct: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL VP GKT+ LNPLKFQGPCKSK+    L G +MAPSKDEW SD  DKW+QF+DI+ L
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120

Query: 121 NVKGAGRFDGQGSFWW-KDCDHKHCKRPTVC----------------------------- 180
            VKG G+FDGQGS WW KDC HKHCKRPT                               
Sbjct: 121 TVKGDGQFDGQGSSWWDKDC-HKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCN 180

Query: 181 ---------------------------------------DDCIAINNGSSNINIIGITCG 240
                                                  DDCIAIN+GSSNI+IIG+TCG
Sbjct: 181 DVIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCG 240

Query: 241 PGHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKN 300
           PGHGISIGSLGK+G+YNVVENV+VS C+LQ TQNGVRIKTW+GGYGYA+NITFEKITL+N
Sbjct: 241 PGHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLEN 300

Query: 301 AKNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILD 325
            KNPIIIDQ+Y++YAY RKNKG +IKVSDVT+R+V+GTSANE AITL+CSRARCTNII++
Sbjct: 301 VKNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIME 360

BLAST of HG10001899 vs. ExPASy TrEMBL
Match: A0A6J1IWN4 (probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.3e-131
Identity = 243/397 (61.21%), Postives = 282/397 (71.03%), Query Frame = 0

Query: 1   MKLLFALVFIFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDN 60
           MKLL + VF+FS  +LSS T   SS FNVLDYGA GNGK DDS+AFLKAWND C +TEDN
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLYVPDGKTYFLNPLKFQGPCKSKQ----LRGTLMAPSKDEWPSDSADKWVQFFDIEGL 120
           PTL VP GKT+ LNPLKFQGPCKS++    L G +MAPSKDEW +D  DKW+QF+DI+ L
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 NVKGAGRFDGQGSFWW-KDCDHKHCKRPTVC----------------------------- 180
            VKG GRFDGQGS WW KDC HKHCKRPT                               
Sbjct: 121 TVKGDGRFDGQGSSWWDKDC-HKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCN 180

Query: 181 ---------------------------------------DDCIAINNGSSNINIIGITCG 240
                                                  DDC+AIN+GSSNI+IIG+TCG
Sbjct: 181 DVIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCG 240

Query: 241 PGHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKN 300
           PGHGISIGSLGKDG+YNVVENV+VS+C+LQ TQNGVRIKTW+GGYGYA+NITFEKITL+N
Sbjct: 241 PGHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLEN 300

Query: 301 AKNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCSRARCTNIILD 325
            KNPIIIDQ+Y++YAY RKNKG +IKVSDVT+R+V+GTSANE AITL+CSRARCTNII++
Sbjct: 301 VKNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIME 360

BLAST of HG10001899 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-73
Identity = 171/386 (44.30%), Postives = 206/386 (53.37%), Query Frame = 0

Query: 11  FSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKT 70
           FS+  L   T  +S+  +V  +GA+G+G TDDS+AFLKAW  VC  T D     VP G T
Sbjct: 9   FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG-QFVVPAGMT 68

Query: 71  YFLNPLKFQGPCKSK----QLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDG 130
           + L PLKFQG CKS     Q+ G L+APSK  W  D  D+W+ F DIEGL ++G G  +G
Sbjct: 69  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEING 128

Query: 131 QGSFWW---------KDCD---------------HKH---CKRPTVC------------- 190
           QGS WW         + C+               H H   C   T+              
Sbjct: 129 QGSSWWEHKGSRPTFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNT 188

Query: 191 ---------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNV 250
                                DDCIAIN+G+SNI+I GI CGPGHGISIGSLGKDGE   
Sbjct: 189 DGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETAT 248

Query: 251 VENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYY----SSY 310
           VENV V NC  +GT NG RIKTWQGG GYAR ITF  ITL N +NPIIIDQ+Y    S  
Sbjct: 249 VENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDN 308

Query: 311 AYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCS-RARCTNIILDDVSIKTTI--PGK 325
           A  RK+    ++VS V F    GTS +E  +   CS R  CT I L D+ I+T     G+
Sbjct: 309 AKDRKSSA--VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQ 368

BLAST of HG10001899 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 171/389 (43.96%), Postives = 206/389 (52.96%), Query Frame = 0

Query: 11  FSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKT 70
           FS+  L   T  +S+  +V  +GA+G+G TDDS+AFLKAW  VC  T D     VP G T
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDG-QFVVPAGMT 69

Query: 71  YFLNPLKFQGPCKSK----QLRGTLMAPSKDEWPSDSADKWVQFFDIEGLNVKGAGRFDG 130
           + L PLKFQG CKS     Q+ G L+APSK  W  D  D+W+ F DIEGL ++G G  +G
Sbjct: 70  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEING 129

Query: 131 QGSFWW------------KDCD---------------HKH---CKRPTVC---------- 190
           QGS WW            + C+               H H   C   T+           
Sbjct: 130 QGSSWWEHKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESS 189

Query: 191 ------------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGE 250
                                   DDCIAIN+G+SNI+I GI CGPGHGISIGSLGKDGE
Sbjct: 190 PNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGE 249

Query: 251 YNVVENVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYY---- 310
              VENV V NC  +GT NG RIKTWQGG GYAR ITF  ITL N +NPIIIDQ+Y    
Sbjct: 250 TATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGD 309

Query: 311 SSYAYSRKNKGMDIKVSDVTFREVNGTSANEDAITLNCS-RARCTNIILDDVSIKTTI-- 325
           S  A  RK+    ++VS V F    GTS +E  +   CS R  CT I L D+ I+T    
Sbjct: 310 SDNAKDRKSSA--VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSG 369

BLAST of HG10001899 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.9e-70
Identity = 142/348 (40.80%), Postives = 191/348 (54.89%), Query Frame = 0

Query: 27  FNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQGPCKSK- 86
           +NVL +GA G+G+TDDS AFL+AWN  CG  E+  TL++P GKTY L P++F+GPCKS  
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  ---QLRGTLMAPSK-DEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWWKDCDHKHCK 146
              QL G ++APS    W +  +  W+ F  + GL + G+G  +G+GS +W+     +C 
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWEALHICNCN 172

Query: 147 RPTV-----------------C-------------------------------------- 206
             T+                 C                                      
Sbjct: 173 NLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYSTNVNIFDSTIQ 232

Query: 207 --DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVENVHVSNCLLQGTQNGVR 266
             DDCIAIN GSS+INI  + CGPGHGIS+GSLG DGE   V +V+V+ C    T NG R
Sbjct: 233 TGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQCTFNKTTNGAR 292

Query: 267 IKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKNKGMDIKVSDVTFREVNG 312
           IKTWQGG GYARNI+FE ITL N +NPIIIDQ Y+        K   + +S V +    G
Sbjct: 293 IKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAISSVKYVGFQG 352

BLAST of HG10001899 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 248.8 bits (634), Expect = 5.9e-66
Identity = 145/364 (39.84%), Postives = 192/364 (52.75%), Query Frame = 0

Query: 21  VLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGKTYFLNPLKFQG 80
           ++   ++NVL++ A G+G+TDDS+AFL+AW   CG   D  TL +P GKT+ L P  FQG
Sbjct: 18  IVYGKNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQG 77

Query: 81  PCKSK----QLRGTLMAPS-KDEWPSDSADKWVQFFDIEGLNVKGAGRFDGQGSFWWK-- 140
           PCKS     QL GT++APS K  W    +  W++F  + GL + G+G  D +GS +W+  
Sbjct: 78  PCKSSSIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELN 137

Query: 141 -------------DCDH------------------KHCKRPTVC---------------- 200
                         CD+                  K C    +                 
Sbjct: 138 LKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGI 197

Query: 201 ------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVEN 260
                             DDCIAIN+GSSNINI GI CGPGHGIS+GSLG  G    V +
Sbjct: 198 DISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSD 257

Query: 261 VHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKNK 312
           V V++C    T NG RIKTW GG GYARNI+F  ITL N KNPIIIDQ+Y      R  +
Sbjct: 258 VQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHYID--KGRLTE 317

BLAST of HG10001899 vs. TAIR 10
Match: AT5G44840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.8e-65
Identity = 142/366 (38.80%), Postives = 198/366 (54.10%), Query Frame = 0

Query: 10  IFSIDILSSCTVLASSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEDNPTLYVPDGK 69
           +F + +L S  V     ++VL++GA GNG TDDSKAF+KAW+ +CG+  ++ T  +P  K
Sbjct: 8   VFLLTLLHSILV-NGQIYDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNK 67

Query: 70  TYFLNPLKFQGPCKSKQLR----GTLMAP-SKDEWPSDSADKWVQFFDIEGLNVKGAGRF 129
           T+ L PL FQGPCKS  ++    G ++AP +K  W      +WV F +I GL V G+G  
Sbjct: 68  TFLLQPLTFQGPCKSPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTI 127

Query: 130 DGQGSFWWKD-----CDH------------------KHCKRPTVC--------------- 189
            G+GS +WK      C+                     CKR  +                
Sbjct: 128 HGRGSSFWKQLHFQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLVAPHDSPNTDG 187

Query: 190 -------------------DDCIAINNGSSNINIIGITCGPGHGISIGSLGKDGEYNVVE 249
                              DDC+AIN+GS NINI  + CGPGHGIS+GS+G+DGE ++VE
Sbjct: 188 INISESSDVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSVGRDGEESIVE 247

Query: 250 NVHVSNCLLQGTQNGVRIKTWQGGYGYARNITFEKITLKNAKNPIIIDQYYSSYAYSRKN 309
           NV V+NC    T NG RIKTW  G GYA+NI F+ +T +  KNPIIIDQ Y         
Sbjct: 248 NVQVTNCTFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQNYVDKGRLDVE 307

Query: 310 KGMDIKVSDVTFREVNGTSANEDAITLNCSRAR-CTNIILDDVSIKTTIPGKEAKAFCQN 313
           +   + +S+VTF ++ GTS  ++ I ++CS+   C +I+LD + I  T+ G +    C N
Sbjct: 308 ESA-VAISNVTFTDLRGTSKLDEIIKIDCSKVTYCKDIVLDKIDI-ATVDGNKPIVECSN 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888676.11.6e-15370.96probable polygalacturonase At3g15720 [Benincasa hispida][more]
KGN53196.13.3e-15169.64hypothetical protein Csa_014667 [Cucumis sativus][more]
XP_031739967.11.8e-14968.94probable polygalacturonase At3g15720 [Cucumis sativus][more]
KAA0044700.12.0e-14567.42putative polygalacturonase [Cucumis melo var. makuwa] >TYK16887.1 putative polyg... [more]
XP_008454473.11.5e-14067.79PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LW071.6e-7143.96Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q94AJ59.5e-5333.51Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q9FY196.8e-5135.11Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q7M1E71.2e-5034.86Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q8RY299.8e-5037.22Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZ951.6e-15169.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1[more]
A0A5D3CYS89.9e-14667.42Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BYT57.3e-14167.79probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=... [more]
A0A6J1GVS63.1e-13161.46probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114579... [more]
A0A6J1IWN45.3e-13161.21probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259... [more]
Match NameE-valueIdentityDescription
AT3G15720.25.0e-7344.30Pectin lyase-like superfamily protein [more]
AT3G15720.11.1e-7243.96Pectin lyase-like superfamily protein [more]
AT5G27530.13.9e-7040.80Pectin lyase-like superfamily protein [more]
AT4G35670.15.9e-6639.84Pectin lyase-like superfamily protein [more]
AT5G44840.13.8e-6538.80Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 215..236
e-value: 1200.0
score: 6.6
coord: 186..207
e-value: 4.9
score: 16.1
coord: 156..176
e-value: 8200.0
score: 0.7
coord: 249..276
e-value: 1500.0
score: 5.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 146..308
e-value: 5.2E-51
score: 173.6
coord: 62..141
e-value: 1.6E-8
score: 34.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 163..176
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 16..145
e-value: 9.0E-28
score: 99.3
coord: 146..319
e-value: 2.0E-53
score: 183.8
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 146..321
coord: 8..144
NoneNo IPR availablePANTHERPTHR31375:SF232SUBFAMILY NOT NAMEDcoord: 146..321
coord: 8..144
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 15..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001899.1HG10001899.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity