HG10001860 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001860
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	gibberellin 20-oxidase-like protein
Location	Chr11: 1146704 .. 1146931 (-)
RNA-Seq Expression	HG10001860
Synteny	HG10001860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGAAGAATCGATTGAGATGTATGAGGAATTACCAGTAATGGACATGGATTGTTGTGAGGAACTGAGCGAATTGAGGCGATCGAGTATGTACAAGGCGTGTAATGAATGGGGGCATTTCTACATCAGAAACCATGGCGTTTCGAAGGAGCTTTACCGGAAACTGAGAGACGTCACCGATGAACTGTTGACGGCGGTGCCGGAAGAGACGAAAGAGGGAAACTGA

mRNA sequence

ATGTCAGAAGAATCGATTGAGATGTATGAGGAATTACCAGTAATGGACATGGATTGTTGTGAGGAACTGAGCGAATTGAGGCGATCGAGTATGTACAAGGCGTGTAATGAATGGGGGCATTTCTACATCAGAAACCATGGCGTTTCGAAGGAGCTTTACCGGAAACTGAGAGACGTCACCGATGAACTGTTGACGGCGGTGCCGGAAGAGACGAAAGAGGGAAACTGA

Coding sequence (CDS)

ATGTCAGAAGAATCGATTGAGATGTATGAGGAATTACCAGTAATGGACATGGATTGTTGTGAGGAACTGAGCGAATTGAGGCGATCGAGTATGTACAAGGCGTGTAATGAATGGGGGCATTTCTACATCAGAAACCATGGCGTTTCGAAGGAGCTTTACCGGAAACTGAGAGACGTCACCGATGAACTGTTGACGGCGGTGCCGGAAGAGACGAAAGAGGGAAACTGA

Protein sequence

MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEETKEGN

Homology

[Close]

BLAST of HG10001860 vs. NCBI nr
Match: XP_004146911.1 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_014948 [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 61/74 (82.43%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM+EEL VMDM+ CE LSE  R S+YKACNEWGHFYI+NHGVSKELY+KLR VT
Sbjct: 1  MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61 DELLTAVPEETKEG 75
          DELLTAVPEETKEG
Sbjct: 61 DELLTAVPEETKEG 74

[Close]

BLAST of HG10001860 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 61/73 (83.56%), Postives = 65/73 (89.04%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM EELPVMDM+CCE LSE  R S+YKACNEWGHFYI+NHGVSKELY KLR VT
Sbjct: 1  MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61 DELLTAVPEETKE 74
          DELLTAVPEE KE
Sbjct: 61 DELLTAVPEEAKE 73

[Close]

BLAST of HG10001860 vs. NCBI nr
Match: KAA0044653.1 (gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 126.3 bits (316), Expect = 1.1e-25
Identity = 61/73 (83.56%), Postives = 65/73 (89.04%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM EELPVMDM+CCE LSE  R S+YKACNEWGHFYI+NHGVSKELY KLR VT
Sbjct: 1  MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61 DELLTAVPEETKE 74
          DELLTAVPEE KE
Sbjct: 61 DELLTAVPEEAKE 73

[Close]

BLAST of HG10001860 vs. NCBI nr
Match: XP_038876797.1 (gibberellin 20-oxidase-like protein isoform X1 [Benincasa hispida])

HSP 1 Score: 125.6 bits (314), Expect = 1.9e-25
Identity = 62/74 (83.78%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MSEESIEMYEELPVMDMDCC++LSE  RS +YKACNEWGHFYIRNHGVSKELY KLR VT
Sbjct: 1  MSEESIEMYEELPVMDMDCCDDLSESSRSCLYKACNEWGHFYIRNHGVSKELYLKLRAVT 60

Query: 61 DELLTAVPEETKEG 75
          DELLT   EE KEG
Sbjct: 61 DELLT---EERKEG 71

[Close]

BLAST of HG10001860 vs. NCBI nr
Match: XP_038876798.1 (gibberellin 20-oxidase-like protein isoform X2 [Benincasa hispida])

HSP 1 Score: 125.6 bits (314), Expect = 1.9e-25
Identity = 62/74 (83.78%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MSEESIEMYEELPVMDMDCC++LSE  RS +YKACNEWGHFYIRNHGVSKELY KLR VT
Sbjct: 1  MSEESIEMYEELPVMDMDCCDDLSESSRSCLYKACNEWGHFYIRNHGVSKELYLKLRAVT 60

Query: 61 DELLTAVPEETKEG 75
          DELLT   EE KEG
Sbjct: 61 DELLT---EERKEG 71

[Close]

BLAST of HG10001860 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-07
Identity = 27/60 (45.00%), Postives = 38/60 (63.33%), Query Frame = 0


Query: 11 ELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEE 70
          ELPV D+   + LSE   +S+  AC EWG FY+ NHGVS+++Y+KLR  +  +     EE
Sbjct: 4  ELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

[Close]

BLAST of HG10001860 vs. ExPASy TrEMBL
Match: A0A5D3D2E7 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00730 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.3e-26
Identity = 61/73 (83.56%), Postives = 65/73 (89.04%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM EELPVMDM+CCE LSE  R S+YKACNEWGHFYI+NHGVSKELY KLR VT
Sbjct: 1  MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61 DELLTAVPEETKE 74
          DELLTAVPEE KE
Sbjct: 61 DELLTAVPEEAKE 73

[Close]

BLAST of HG10001860 vs. ExPASy TrEMBL
Match: A0A5A7TME4 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004080 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.3e-26
Identity = 61/73 (83.56%), Postives = 65/73 (89.04%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM EELPVMDM+CCE LSE  R S+YKACNEWGHFYI+NHGVSKELY KLR VT
Sbjct: 1  MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61 DELLTAVPEETKE 74
          DELLTAVPEE KE
Sbjct: 61 DELLTAVPEEAKE 73

[Close]

BLAST of HG10001860 vs. ExPASy TrEMBL
Match: A0A0A0KW97 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.3e-26
Identity = 61/74 (82.43%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM+EEL VMDM+ CE LSE  R S+YKACNEWGHFYI+NHGVSKELY+KLR VT
Sbjct: 1  MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61 DELLTAVPEETKEG 75
          DELLTAVPEETKEG
Sbjct: 61 DELLTAVPEETKEG 74

[Close]

BLAST of HG10001860 vs. ExPASy TrEMBL
Match: A0A1S3BYJ4 (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.3e-26
Identity = 61/73 (83.56%), Postives = 65/73 (89.04%), Query Frame = 0


Query: 1  MSEESIEMYEELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVT 60
          MS+ESIEM EELPVMDM+CCE LSE  R S+YKACNEWGHFYI+NHGVSKELY KLR VT
Sbjct: 1  MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61 DELLTAVPEETKE 74
          DELLTAVPEE KE
Sbjct: 61 DELLTAVPEEAKE 73

[Close]

BLAST of HG10001860 vs. ExPASy TrEMBL
Match: A0A6J1CJY7 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011696 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.2e-09
Identity = 37/62 (59.68%), Postives = 45/62 (72.58%), Query Frame = 0


Query: 11 ELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEE 70
          ELPV+D+   E+LSE   SS+  ACNEWG FYIRNHGVSKE Y +L  + DEL   +P+E
Sbjct: 9  ELPVIDV--YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF-RLPQE 67

Query: 71 TK 73
          TK
Sbjct: 69 TK 67

[Close]

BLAST of HG10001860 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-08
Identity = 27/60 (45.00%), Postives = 38/60 (63.33%), Query Frame = 0


Query: 11 ELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEE 70
          ELPV D+   + LSE   +S+  AC EWG FY+ NHGVS+++Y+KLR  +  +     EE
Sbjct: 4  ELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

[Close]

BLAST of HG10001860 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.4e-07
Identity = 27/62 (43.55%), Postives = 42/62 (67.74%), Query Frame = 0


Query: 11 ELPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEE 70
          +LPV+D+    E S L  SS+ +AC EWG FY+ NHG+SKE++ K+  ++ ++  A P E
Sbjct: 9  QLPVLDLTQPIESSIL--SSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA-PLE 67

Query: 71 TK 73
          +K
Sbjct: 69 SK 67

[Close]

BLAST of HG10001860 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 1.1e-04
Identity = 25/71 (35.21%), Postives = 41/71 (57.75%), Query Frame = 0


Query: 12  LPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDV---------TDE 71
           +PV+D+   +E  E  R ++ KA  EWG F + NHG+  EL R+L+DV         +++
Sbjct: 43  IPVVDLSDPDE--ESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEK 102

Query: 72  LLTAVPEETKE 74
              A PE++K+
Sbjct: 103 ESVAKPEDSKD 111

[Close]

BLAST of HG10001860 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 43.1 bits (100), Expect = 1.1e-04
Identity = 25/71 (35.21%), Postives = 41/71 (57.75%), Query Frame = 0


Query: 12  LPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDV---------TDE 71
           +PV+D+   +E  E  R ++ KA  EWG F + NHG+  EL R+L+DV         +++
Sbjct: 43  IPVVDLSDPDE--ESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEK 102

Query: 72  LLTAVPEETKE 74
              A PE++K+
Sbjct: 103 ESVAKPEDSKD 111

[Close]

BLAST of HG10001860 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 1.9e-04
Identity = 21/62 (33.87%), Postives = 36/62 (58.06%), Query Frame = 0


Query: 12 LPVMDMDCCEELSELRRSSMYKACNEWGHFYIRNHGVSKELYRKLRDVTDELLTAVPEET 71
          +PV+D      L  L +  + +AC EWG F + NHGV   L  +L+++++ LL+   E+ 
Sbjct: 34 IPVID------LERLDKEILREACKEWGIFRLENHGVPLALTSRLQEISESLLSLPFEKK 89

Query: 72 KE 74
          +E
Sbjct: 94 RE 89

The following BLAST results are available for this feature:

BLAST of HG10001860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146911.1	1.1e-25	82.43	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical p...	[more]
XP_008453897.1	1.1e-25	83.56	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberell...	[more]
KAA0044653.1	1.1e-25	83.56	gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa]	[more]
XP_038876797.1	1.9e-25	83.78	gibberellin 20-oxidase-like protein isoform X1 [Benincasa hispida]	[more]
XP_038876798.1	1.9e-25	83.78	gibberellin 20-oxidase-like protein isoform X2 [Benincasa hispida]	[more]

BLAST of HG10001860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	1.8e-07	45.00	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]

BLAST of HG10001860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D2E7	5.3e-26	83.56	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7TME4	5.3e-26	83.56	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A0A0KW97	5.3e-26	82.43	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A1S3BYJ4	5.3e-26	83.56	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
A0A6J1CJY7	1.2e-09	59.68	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110116...	[more]

BLAST of HG10001860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51310.1	1.3e-08	45.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.1	2.4e-07	43.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G08640.1	1.1e-04	35.21	flavonol synthase 1	[more]
AT5G08640.2	1.1e-04	35.21	flavonol synthase 1	[more]
AT5G58660.1	1.9e-04	33.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..75 e-value: 4.6E-10 score: 41.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 12..73 e-value: 1.6E-10 score: 41.8
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 5..72
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..72
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001860.1	HG10001860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding