HG10001660 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001660
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	glucan endo-1,3-beta-glucosidase 3-like
Location	Chr09: 19208930 .. 19209556 (+)
RNA-Seq Expression	HG10001660
Synteny	HG10001660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAACCAGAAAGCAAGCACACACATTACAAGAATAACAAAACAAAACAAAACAAAGAATTTCAATTCATTGACAAAGAAAACTAAAATCAGTTCATTGTTTTGTGTGTACCTTTTCTTTTCCCCCAATTTGAAACCAGGTTCATCAAGAGCAGATCCCACACAAAAACAGGGGCAACAATTCAGAGCAGCATCCAGAAAATCTTCAACCAATCAAAAGGACATCACGACCCCAATAACGACAGTCCCAACAATCAACATCCCAACCATTCCCATCATAAACCCTACCAGTTCAAACCCCGACACCGTCTCCCCAACCATGACAACCCCAAGCTTTACACCGGCAACCACCGCGAGCGGTGGCTCCAGCTGGTGCATTGCGAGTCTAAGCGCGTCGCAGGCGGCGCTGCAATTAGCTCTGGACTACGCCTGCGGCATGGGCGGTGCCGATTGCTCGTCGATTCAAGCCGGCGGAAACTGCTACAACCCTAATTCGGTTCGAGACCATGCTTCTTACGCTTTCAATAGCTATTATCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACCAATCCCAGTAAGTTTCTCTTTCAAGCTTAA

mRNA sequence

ATGCGTAACCAGAAAGCAAGCACACACATTACAAGAATAACAAAACAAAACAAAACAAAGAATTTCAATTCATTGACAAAGAAAACTAAAATCAGTTCATTGTTTTGTGTGTACCTTTTCTTTTCCCCCAATTTGAAACCAGGTTCATCAAGAGCAGATCCCACACAAAAACAGGGGCAACAATTCAGAGCAGCATCCAGAAAATCTTCAACCAATCAAAAGGACATCACGACCCCAATAACGACAGTCCCAACAATCAACATCCCAACCATTCCCATCATAAACCCTACCAGTTCAAACCCCGACACCGTCTCCCCAACCATGACAACCCCAAGCTTTACACCGGCAACCACCGCGAGCGGTGGCTCCAGCTGGTGCATTGCGAGTCTAAGCGCGTCGCAGGCGGCGCTGCAATTAGCTCTGGACTACGCCTGCGGCATGGGCGGTGCCGATTGCTCGTCGATTCAAGCCGGCGGAAACTGCTACAACCCTAATTCGGTTCGAGACCATGCTTCTTACGCTTTCAATAGCTATTATCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACCAATCCCAGTAAGTTTCTCTTTCAAGCTTAA

Coding sequence (CDS)

ATGCGTAACCAGAAAGCAAGCACACACATTACAAGAATAACAAAACAAAACAAAACAAAGAATTTCAATTCATTGACAAAGAAAACTAAAATCAGTTCATTGTTTTGTGTGTACCTTTTCTTTTCCCCCAATTTGAAACCAGGTTCATCAAGAGCAGATCCCACACAAAAACAGGGGCAACAATTCAGAGCAGCATCCAGAAAATCTTCAACCAATCAAAAGGACATCACGACCCCAATAACGACAGTCCCAACAATCAACATCCCAACCATTCCCATCATAAACCCTACCAGTTCAAACCCCGACACCGTCTCCCCAACCATGACAACCCCAAGCTTTACACCGGCAACCACCGCGAGCGGTGGCTCCAGCTGGTGCATTGCGAGTCTAAGCGCGTCGCAGGCGGCGCTGCAATTAGCTCTGGACTACGCCTGCGGCATGGGCGGTGCCGATTGCTCGTCGATTCAAGCCGGCGGAAACTGCTACAACCCTAATTCGGTTCGAGACCATGCTTCTTACGCTTTCAATAGCTATTATCAGAAGAATCCGCTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACCAATCCCAGTAAGTTTCTCTTTCAAGCTTAA

Protein sequence

MRNQKASTHITRITKQNKTKNFNSLTKKTKISSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPTIPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSKFLFQA

Homology

[Close]

BLAST of HG10001660 vs. NCBI nr
Match: XP_038900831.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-76
Identity = 162/203 (79.80%), Postives = 176/203 (86.70%), Query Frame = 0


Query: 1   MRNQKASTHITRITKQNKTKNFNSLTKKTKISSLFCVYLFFSPNLKPGSSRADPTQKQGQ 60
           M +QKA  +I+   ++ K +      ++ K+  L CVYL FSPNLK GSSR DPTQKQGQ
Sbjct: 2   MHSQKA--NISHTLQETKQRISIHRQRELKLDLLSCVYLSFSPNLKSGSSRTDPTQKQGQ 61

Query: 61  QFRAASRKSSTNQKDITTPITTVPTINIPTIPIINPTSSNPDTVSPTMTTPSFTPA-TTA 120
           QF    RKSS+NQKDITTPITTVPT+NIPT+PIINPTSSNPDTVSP MTTPSFTPA TTA
Sbjct: 62  QF----RKSSSNQKDITTPITTVPTVNIPTMPIINPTSSNPDTVSPAMTTPSFTPATTTA 121

Query: 121 SGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYY 180
           SGGSSWCIAS+SASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYY
Sbjct: 122 SGGSSWCIASVSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYY 181

Query: 181 QKNPLPNSCNFGGTAVITSTNPS 203
           QKNPLPNSCNFGGTA+ITSTNPS
Sbjct: 182 QKNPLPNSCNFGGTAMITSTNPS 198

[Close]

BLAST of HG10001660 vs. NCBI nr
Match: KAA0054595.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo var. makuwa] >TYK27559.1 PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 290.8 bits (743), Expect = 9.3e-75
Identity = 153/174 (87.93%), Postives = 159/174 (91.38%), Query Frame = 0


Query: 31  ISSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPT 90
           I S   ++LF      PGSSRADP QKQG+QFRAA RKSST+QKDITTPITTVPTINIPT
Sbjct: 6   IYSTLILFLFIH-CFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 65

Query: 91  IPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGA 150
           IPIINPTSSNPDTVSP MTTPSFTPATT +GGSSWCIAS SASQAALQLALDYACGMGGA
Sbjct: 66  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 125

Query: 151 DCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSKF 205
           DCSSIQAGGNCYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSKF
Sbjct: 126 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSKF 178

[Close]

BLAST of HG10001660 vs. NCBI nr
Match: XP_008456350.1 (PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo] >XP_016901925.1 PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo])

HSP 1 Score: 286.6 bits (732), Expect = 1.7e-73
Identity = 151/172 (87.79%), Postives = 157/172 (91.28%), Query Frame = 0


Query: 31  ISSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPT 90
           I S   ++LF      PGSSRADP QKQG+QFRAA RKSST+QKDITTPITTVPTINIPT
Sbjct: 6   IYSTLILFLFIH-CFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 65

Query: 91  IPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGA 150
           IPIINPTSSNPDTVSP MTTPSFTPATT +GGSSWCIAS SASQAALQLALDYACGMGGA
Sbjct: 66  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 125

Query: 151 DCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           DCSSIQAGGNCYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 126 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPS 176

[Close]

BLAST of HG10001660 vs. NCBI nr
Match: XP_011656991.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 isoform X1 [Cucumis sativus] >KAE8646882.1 hypothetical protein Csa_021032 [Cucumis sativus])

HSP 1 Score: 285.8 bits (730), Expect = 3.0e-73
Identity = 147/156 (94.23%), Postives = 150/156 (96.15%), Query Frame = 0


Query: 47  PGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPTIPIINPTSSNPDTVSP 106
           PGSSRADPTQKQG+QFRAASRKSST QKDITTPITTVPTINIPTIPIINP SSNPDTVSP
Sbjct: 21  PGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVPTINIPTIPIINPASSNPDTVSP 80

Query: 107 TMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNS 166
            MTTPSFTP+TT SGGSSWCIAS SASQAALQLALDYACG+GGADCSSIQ GGNCYNPNS
Sbjct: 81  AMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYACGIGGADCSSIQGGGNCYNPNS 140

Query: 167 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 141 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 176

[Close]

BLAST of HG10001660 vs. NCBI nr
Match: XP_011656992.1 (major pollen allergen Ole e 10 isoform X2 [Cucumis sativus])

HSP 1 Score: 285.8 bits (730), Expect = 3.0e-73
Identity = 147/156 (94.23%), Postives = 150/156 (96.15%), Query Frame = 0


Query: 47  PGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPTIPIINPTSSNPDTVSP 106
           PGSSRADPTQKQG+QFRAASRKSST QKDITTPITTVPTINIPTIPIINP SSNPDTVSP
Sbjct: 21  PGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVPTINIPTIPIINPASSNPDTVSP 80

Query: 107 TMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNS 166
            MTTPSFTP+TT SGGSSWCIAS SASQAALQLALDYACG+GGADCSSIQ GGNCYNPNS
Sbjct: 81  AMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYACGIGGADCSSIQGGGNCYNPNS 140

Query: 167 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 141 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 176

[Close]

BLAST of HG10001660 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.1e-17
Identity = 52/119 (43.70%), Postives = 64/119 (53.78%), Query Frame = 0


Query: 85  TINIPTIPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYA 144
           T  +P  P+  PT S P + SP        P     G   WC+    A+ A LQ  +DY 
Sbjct: 4   TAGVPDQPVPTPTPSVPTSSSP-------VPKPPTQGNKKWCVPKAEATDAQLQSNIDYV 63

Query: 145 CGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQ-KNPLPNSCNFGGTAVITSTNPS 203
           C   G DC  IQA G C+NPN+VR HASYA NS+YQ K      C+F GT  ITS++PS
Sbjct: 64  CSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPS 115

[Close]

BLAST of HG10001660 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 43/80 (53.75%), Postives = 56/80 (70.00%), Query Frame = 0


Query: 124 SWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQK-N 183
           ++CIA        LQ ALD+ACG G ++CS IQ G +CY PN+V+ HAS+AFNSYYQK  
Sbjct: 380 TYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEG 439

Query: 184 PLPNSCNFGGTAVITSTNPS 203
               SC+F G A+IT+T+PS
Sbjct: 440 RASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of HG10001660 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-15
Identity = 39/80 (48.75%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 125 WCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQKN-P 184
           WC+A  +A  ++LQ A+++ACG GGADC  IQ GG C +P  V+  AS+ FN+YY KN  
Sbjct: 41  WCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFVFNNYYLKNGE 100

Query: 185 LPNSCNFGGTAVITSTNPSK 204
              +CNF   A +TS NPS+
Sbjct: 101 EDEACNFNNNAALTSLNPSQ 120

[Close]

BLAST of HG10001660 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.6e-15
Identity = 40/81 (49.38%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 123 SSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQ-K 182
           +SWC+     S + LQ  LDYACG  GADC+     G+C+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACG-NGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKK 78

Query: 183 NPLPNSCNFGGTAVITSTNPS 203
                SCNF GTA +T+T+PS
Sbjct: 79  GQASESCNFTGTATLTTTDPS 98

[Close]

BLAST of HG10001660 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 45/104 (43.27%), Postives = 68/104 (65.38%), Query Frame = 0


Query: 103 TVSPTMT---TPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGG 162
           +V+PT +   +P+ +P    SGG  WC+A   A+   LQ  ++YACG    DC  IQ+GG
Sbjct: 352 SVAPTPSPNPSPNPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF--VDCKPIQSGG 411

Query: 163 NCYNPNSVRDHASYAFNSYYQKNPLPN-SCNFGGTAVITSTNPS 203
            C++PNS++ HASY  N+YYQ N   + +C+F GT ++TS++PS
Sbjct: 412 ACFSPNSLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPS 453

[Close]

BLAST of HG10001660 vs. ExPASy TrEMBL
Match: A0A5A7ULQ0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold22G00320 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.5e-75
Identity = 153/174 (87.93%), Postives = 159/174 (91.38%), Query Frame = 0


Query: 31  ISSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPT 90
           I S   ++LF      PGSSRADP QKQG+QFRAA RKSST+QKDITTPITTVPTINIPT
Sbjct: 6   IYSTLILFLFIH-CFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 65

Query: 91  IPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGA 150
           IPIINPTSSNPDTVSP MTTPSFTPATT +GGSSWCIAS SASQAALQLALDYACGMGGA
Sbjct: 66  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 125

Query: 151 DCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSKF 205
           DCSSIQAGGNCYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSKF
Sbjct: 126 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSKF 178

[Close]

BLAST of HG10001660 vs. ExPASy TrEMBL
Match: A0A1S4E1R5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC103496317 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.5e-74
Identity = 151/172 (87.79%), Postives = 157/172 (91.28%), Query Frame = 0


Query: 31  ISSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPT 90
           I S   ++LF      PGSSRADP QKQG+QFRAA RKSST+QKDITTPITTVPTINIPT
Sbjct: 6   IYSTLILFLFIH-CFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVPTINIPT 65

Query: 91  IPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGA 150
           IPIINPTSSNPDTVSP MTTPSFTPATT +GGSSWCIAS SASQAALQLALDYACGMGGA
Sbjct: 66  IPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYACGMGGA 125

Query: 151 DCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           DCSSIQAGGNCYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 126 DCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPS 176

[Close]

BLAST of HG10001660 vs. ExPASy TrEMBL
Match: A0A0A0KDA3 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.4e-73
Identity = 147/156 (94.23%), Postives = 150/156 (96.15%), Query Frame = 0


Query: 47  PGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPTIPIINPTSSNPDTVSP 106
           PGSSRADPTQKQG+QFRAASRKSST QKDITTPITTVPTINIPTIPIINP SSNPDTVSP
Sbjct: 21  PGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVPTINIPTIPIINPASSNPDTVSP 80

Query: 107 TMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNS 166
            MTTPSFTP+TT SGGSSWCIAS SASQAALQLALDYACG+GGADCSSIQ GGNCYNPNS
Sbjct: 81  AMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYACGIGGADCSSIQGGGNCYNPNS 140

Query: 167 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 141 VRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 176

[Close]

BLAST of HG10001660 vs. ExPASy TrEMBL
Match: A0A6J1FWZ7 (major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC111448235 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.1e-62
Identity = 136/171 (79.53%), Postives = 147/171 (85.96%), Query Frame = 0


Query: 32  SSLFCVYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVPTINIPTI 91
           ++L      F   L PGSSRADPTQKQG ++RAA   SST+QKDITTPITTVPTINI   
Sbjct: 8   TTLILFLFLFIHCLFPGSSRADPTQKQG-KYRAA---SSTHQKDITTPITTVPTINI--- 67

Query: 92  PIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGAD 151
               PT+SNPD+VSPT+TTPSFTPATTA+GGSSWCIASL ASQA LQLALDYACGMGGAD
Sbjct: 68  ----PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGAD 127

Query: 152 CSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           CS+IQ GG+CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 128 CSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 167

[Close]

BLAST of HG10001660 vs. ExPASy TrEMBL
Match: A0A6J1JFA6 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 9.7e-62
Identity = 139/178 (78.09%), Postives = 150/178 (84.27%), Query Frame = 0


Query: 26  TKKTKISSLFC-VYLFFSPNLKPGSSRADPTQKQGQQFRAASRKSSTNQKDITTPITTVP 85
           T  T I  LF  ++ FF     PGSSRADPTQKQG ++RAA   SST+QKDITTPITTVP
Sbjct: 6   TCTTLILFLFLFIHCFF-----PGSSRADPTQKQG-KYRAA---SSTHQKDITTPITTVP 65

Query: 86  TINIPTIPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYA 145
           TINI       PT+SNPD+VSPT+TTPSFTPATT +GGSSWCIA+L ASQAALQLALDYA
Sbjct: 66  TINI-------PTTSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYA 125

Query: 146 CGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           CGMGGADCS+IQ GG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS
Sbjct: 126 CGMGGADCSAIQTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 167

[Close]

BLAST of HG10001660 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.6e-32
Identity = 73/130 (56.15%), Postives = 89/130 (68.46%), Query Frame = 0


Query: 73  QKDITTPITTVPTINIPTIPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSA 132
           +KDITTP+ T PT   PT  + N  S      +  +T PS        G  SWC+A  + 
Sbjct: 31  EKDITTPLATNPT-TTPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENV 90

Query: 133 SQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGG 192
           ++ ALQ ALDYACG+GGADCS IQ GGNCYNPNS+R HAS+AFNSYYQKNP+P+SCNF G
Sbjct: 91  AKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDG 150

Query: 193 TAVITSTNPS 203
           TA+  S +PS
Sbjct: 151 TAITISADPS 159

[Close]

BLAST of HG10001660 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.6e-32
Identity = 73/130 (56.15%), Postives = 89/130 (68.46%), Query Frame = 0


Query: 73  QKDITTPITTVPTINIPTIPIINPTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSA 132
           +KDITTP+ T PT   PT  + N  S      +  +T PS        G  SWC+A  + 
Sbjct: 31  EKDITTPLATNPT-TTPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENV 90

Query: 133 SQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGG 192
           ++ ALQ ALDYACG+GGADCS IQ GGNCYNPNS+R HAS+AFNSYYQKNP+P+SCNF G
Sbjct: 91  AKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDG 150

Query: 193 TAVITSTNPS 203
           TA+  S +PS
Sbjct: 151 TAITISADPS 159

[Close]

BLAST of HG10001660 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 5.9e-27
Identity = 57/90 (63.33%), Postives = 67/90 (74.44%), Query Frame = 0


Query: 114 TPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHASY 173
           T A    G   WCIA  +AS  +LQ+ALDYACG GGADC  IQ G  CY PN++RDHAS+
Sbjct: 137 TGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASF 196

Query: 174 AFNSYYQKNPLPNSCNFGGTAVITSTNPSK 204
           AFNSYYQK+P  +SCNFGG A +TST+PSK
Sbjct: 197 AFNSYYQKHPGSDSCNFGGAAQLTSTDPSK 226

[Close]

BLAST of HG10001660 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-25
Identity = 71/151 (47.02%), Postives = 90/151 (59.60%), Query Frame = 0


Query: 76  ITTPITTVP----------TINIPTI------PIINPTSSNPDTVSPTMTTPSFTPA--- 135
           I  P+TT+P          TI  PT+      P+ NP +  P T  P+ T P   P    
Sbjct: 65  IVPPVTTIPPPTLTPPPVITIPPPTLTPPVTNPVTNPVTQYPPT-QPSGTVPVPVPVVAP 124

Query: 136 -----TTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSIQAGGNCYNPNSVRDHA 195
                + +  G SWC+A   ASQ +LQ ALDYACG+  ADCS +Q GGNCY+P S++ HA
Sbjct: 125 PVVSNSPSVSGQSWCVAKPGASQVSLQQALDYACGI--ADCSQLQQGGNCYSPISLQSHA 184

Query: 196 SYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 203
           S+AFNSYYQKNP P SC+FGG A + +TNPS
Sbjct: 185 SFAFNSYYQKNPSPQSCDFGGAASLVNTNPS 212

[Close]

BLAST of HG10001660 vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 54/109 (49.54%), Postives = 64/109 (58.72%), Query Frame = 0


Query: 96  PTSSNPDTVSPTMTTPSFTPATTASGGSSWCIASLSASQAALQLALDYACGMGGADCSSI 155
           P + N + V P    PS         G +WC+A+   ++  LQ  LDYACG GGADC  I
Sbjct: 352 PVNDNKEKVVP--VKPSLV-------GQTWCVANGKTTKEKLQEGLDYACGEGGADCRPI 411

Query: 156 QAGGNCYNPNSVRDHASYAFNSYYQKNPL-PNSCNFGGTAVITSTNPSK 204
           Q G  CYNP S+  HASYAFNSYYQKN     +CNFGG A + S  PSK
Sbjct: 412 QPGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPSK 451

The following BLAST results are available for this feature:

BLAST of HG10001660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900831.1	1.3e-76	79.80	PLASMODESMATA CALLOSE-BINDING PROTEIN 3-like isoform X1 [Benincasa hispida]	[more]
KAA0054595.1	9.3e-75	87.93	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo var. makuwa] >TYK2755...	[more]
XP_008456350.1	1.7e-73	87.79	PREDICTED: PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucumis melo] >XP_01690...	[more]
XP_011656991.1	3.0e-73	94.23	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 isoform X1 [Cucumis sativus] >KAE8646882...	[more]
XP_011656992.1	3.0e-73	94.23	major pollen allergen Ole e 10 isoform X2 [Cucumis sativus]	[more]

BLAST of HG10001660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84V39	4.1e-17	43.70	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
O65399	1.0e-15	53.75	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9M2K6	5.0e-15	48.75	PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5...	[more]
Q9SD84	8.6e-15	49.38	PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2...	[more]
P52409	1.1e-14	43.27	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]

BLAST of HG10001660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7ULQ0	4.5e-75	87.93	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S4E1R5	8.5e-74	87.79	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A0A0KDA3	1.4e-73	94.23	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=...	[more]
A0A6J1FWZ7	1.1e-62	79.53	major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC11144823...	[more]
A0A6J1JFA6	9.7e-62	78.09	major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 ...	[more]

BLAST of HG10001660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30933.1	1.6e-32	56.15	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	1.6e-32	56.15	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	5.9e-27	63.33	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	1.9e-25	47.02	Carbohydrate-binding X8 domain superfamily protein	[more]
AT5G55180.2	1.2e-19	49.54	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 124..204 e-value: 2.9E-39 score: 146.4
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 125..194 e-value: 1.8E-20 score: 73.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 119..206 e-value: 2.3E-11 score: 45.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..76
None	No IPR available	PANTHER	PTHR32227:SF338	BETA 1,3-GLUCANASE-LIKE	coord: 48..202
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 48..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001660.1	HG10001660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds