HG10001257 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001257
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	DNA polymerase epsilon subunit 3
Location	Chr09: 15471628 .. 15473807 (+)
RNA-Seq Expression	HG10001257
Synteny	HG10001257

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAAAAAGTAGGTGCCGGGACTGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGGTCGAATCTTCATCCATTATCTCTCTGCTACGTAAGCTCCGCAACCTGCCCTAATTTGTTTAATTCCGAATCAAATTCTATCCATTCTGATGTTCGAAACTAGGGCTCGTCGTTTCTGTGCTCTTTACCTTTAAAACGAAAGCGAAACAGATTAAGGCGAGAAATCTATTGTTGTTATGGCGCTAGGGTTAAGAAAATGTTGTGTATTGGTTAATTCTTGCGTTCGTATTAAGTGGTAGATCTCTAAGCAGGTCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTAAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGTCTCTTAAGGCTTCCCTCAATGGTGAGTAAGCTTTCCACCGCTGTGTATACATTTTCTTTTAAAGAAAAAAAAACCATTTCTCATTGGAGCCTGAAATTGCACGCTTAATTGGAGAATTATGTTTTTGATAGTTTTCACTTGCTAGACATTGTTCAAAAGGATAGTATAAAGCTTCTCTTCGACCTGCAGTACAGTTGCGCGTTAGCTAAACACATGAATCTTATTTCCGATGAAGTTTTCTGTTTTACCCTTTCTTTACTATTTAAATGCTAGCATGGTCTAGTCTTTATGCGATTGCTTGATTTCCCTGCCTAAAAATGCCTTGTATCTCTAGACATTCAATCTCGACTTTAGATCATTTTCAGTAACACTTCAAGAGTGTGAGGTATGCACGGTTAAACGAAGCCCTATGCAAAGAGGATGTTGACATTATAAGACCAATCCTTATCGGTTCGAACATACAAGATGAGTGAATTTGGTATTGTGGTAGGAATAGGACAGACGTTATCAATGTGAAAAGTGGATACAAGTTGCAAATGAATATTTAATGCAAGTCTGAATGACTCTGCCGTTAGTTTCATTAGTTGAGTTACGCATGTAATATAAAAGGCTGCATGAAATTCATCTCAGTTCTCTTAGTTTTACTTGATTGAATTTCGATTCTGAGAGAGCTGGTGAGATCTCAAAGAACTCATGCACACCTTTTATTAAAGGATTATATGATTTGATTATATTAAATAAGCTCCTACGAAATATATCTTCGATGCTTATGAGTCCTTGGAAGTTTTTGTTTGATGTCTTTTTATTTACTTGTATCCAGAAATGCTATTGAAAAATTGAGTTCTTTAAGCTTTGCTATATAGCGATGAGTTAGAGTTGGGATTTACTTTCTGGATTTCTTTTAGAAGTACATACTTAGAGCGCTCCAAGGAGTATGTAAAGTGAGCAATAACGAGGTTTTGTTTATTGAATTCTGGAATAGTGGTATTCACAATGTGCTTTAAGTGTTTCTAATTTCGTTTGTAGTTGGATATATGAGGATATTGAGTCTAATCACACTTTACGCTCTTGGATGTAGAGATGCTTTCTTAATGCATATATAGAAAGATCTATAAATTCTAATGTTACAATACAAGTTCAATGTGTAGATACTTTTCTTATCTGTTCTCTTGATTAGCATTATGTTATCTTTGTTGCTTGCTTAATTATTAGTTGGTCATGTGACAGTATGTCTATTCTTGTTGGATTGTATATATGAATGTCATAATGTCGAAGTATTTCGTTTGAAGTCTGAACAATTGCCGTGCTTAATTTAACGGAAAAGATGTGATATTCAATTGCGTGCAGAAAGCAATATTAATATCCACAGGCTATGATTTTCTATCTTATGGTCACCAAATTCGTGTTCATACTTATCCAAGTTTAGTATTAAGATAAGCAGCTGAACCTTTTTAATGATTTGTTCATTGTGCAACCTTTGCTTGGCTGAATGTAGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGCGAAAAAAAAGAGGAAAGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGCATAGATGGCATCGGAAACAATGATAATGATGAAGAGGAAGAGGAAGAGGAAGAGGAAGATGAAAATGGCGCCAGTGATAACTGA

mRNA sequence

ATGGAGAAAAAAGTAGGTGCCGGGACTGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGGTCGAATCTTCATCCATTATCTCTCTGCTACGTCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTAAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGTCTCTTAAGGCTTCCCTCAATGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGCGAAAAAAAAGAGGAAAGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGCATAGATGGCATCGGAAACAATGATAATGATGAAGAGGAAGAGGAAGAGGAAGAGGAAGATGAAAATGGCGCCAGTGATAACTGA

Coding sequence (CDS)

ATGGAGAAAAAAGTAGGTGCCGGGACTGAAGAGCTTCCGAAGACTATAGTTCGCCGAGTGGTCAAAGAGAAGCTCTCTCAGTGCTCTCGGGACCAGGACATCTCCGTCAACAAAGACTCGCTTCTTGCCTTTTGCGAGAGCGGTCGAATCTTCATCCATTATCTCTCTGCTACGTCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTAAAAGCTCTAGAAGATATGGAATTTCCCGAATTGGTTAGGTCTCTTAAGGCTTCCCTCAATGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCGTCCAGAGAAAAAGAAGCGAAAAAAAAGAGGAAAGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGCATAGATGGCATCGGAAACAATGATAATGATGAAGAGGAAGAGGAAGAGGAAGAGGAAGATGAAAATGGCGCCAGTGATAACTGA

Protein sequence

MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN

Homology

[Close]

BLAST of HG10001257 vs. NCBI nr
Match: XP_004140162.1 (DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymerase epsilon subunit D [Cucumis sativus] >KGN48033.1 hypothetical protein Csa_004222 [Cucumis sativus])

HSP 1 Score: 256.1 bits (653), Expect = 2.0e-64
Identity = 143/161 (88.82%), Postives = 153/161 (95.03%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKK+G G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCES RIFIHYLSAT+N
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASL+EFRRKNAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN 162
           KVEEPTVEGTDGESID +GN+DND + +E+E+EDEN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDND-DNDEDEDEDENRASDD 160

[Close]

BLAST of HG10001257 vs. NCBI nr
Match: XP_038900596.1 (DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymerase epsilon subunit 3 [Benincasa hispida])

HSP 1 Score: 255.0 bits (650), Expect = 4.4e-64
Identity = 142/161 (88.20%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKK+GA  EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCES RIFIHYLSATS 
Sbjct: 1   MEKKIGAAIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSY 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFRRKNAGKKAAASREKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAASREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN 162
           K+EEPTVEGTDGES+DG+GN+DND    E+  EDENGASD+
Sbjct: 121 KIEEPTVEGTDGESVDGVGNDDND----EDGNEDENGASDD 157

[Close]

BLAST of HG10001257 vs. NCBI nr
Match: XP_008449581.1 (PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo])

HSP 1 Score: 252.7 bits (644), Expect = 2.2e-63
Identity = 142/161 (88.20%), Postives = 148/161 (91.93%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKK+ AG EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCES RIFIHYLSAT+N
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFRRKNAGKKAAA REKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN 162
           KVEEPTVEGTDGESIDG+G++DND      E+EDEN ASDN
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDND------EDEDENEASDN 155

[Close]

BLAST of HG10001257 vs. NCBI nr
Match: KAG7035929.1 (DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235.3 bits (599), Expect = 3.6e-58
Identity = 138/165 (83.64%), Postives = 146/165 (88.48%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKKVGA  EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCES RIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIDGIGN----NDNDEEEEEEEEEDENGASDN 162
             EEPTVEGTDGES D + N    ND DE+E E+E+E ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDKDEDEVEDEDEVENGGSDD 165

[Close]

BLAST of HG10001257 vs. NCBI nr
Match: XP_022152961.1 (DNA polymerase epsilon subunit 3 [Momordica charantia])

HSP 1 Score: 235.0 bits (598), Expect = 4.7e-58
Identity = 138/166 (83.13%), Postives = 148/166 (89.16%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKKV A  EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCES RIFIHYLSATSN
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVR LKASLNEFRRK+AGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDN------DEEEEEEEEEDENGASD 161
           K+EEPTVE    ES D +GN+DN      DE+E E+E+E+ENGASD
Sbjct: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASD 163

[Close]

BLAST of HG10001257 vs. ExPASy Swiss-Prot
Match: Q3SZN5 (DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 50/145 (34.48%), Postives = 84/145 (57.93%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +  +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V  LK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIDGIGNNDNDEEEEEEEEEDEN 157
            E  D   + DNDE+EE  EEE++N
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQN 140

[Close]

BLAST of HG10001257 vs. ExPASy Swiss-Prot
Match: Q9NRF9 (DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 50/145 (34.48%), Postives = 84/145 (57.93%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +  +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V  LK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIDGIGNNDNDEEEEEEEEEDEN 157
            E  D   + DNDE+EE  EEE++N
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQN 140

[Close]

BLAST of HG10001257 vs. ExPASy Swiss-Prot
Match: Q5R4W3 (DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-12
Identity = 50/145 (34.48%), Postives = 84/145 (57.93%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +  +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V  LK +L  +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIDGIGNNDNDEEEEEEEEEDEN 157
            E  D   + DNDE+EE  EEE++N
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQN 140

[Close]

BLAST of HG10001257 vs. ExPASy Swiss-Prot
Match: Q9JKP7 (DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.9e-10
Identity = 48/149 (32.21%), Postives = 83/149 (55.70%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +  +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEP----TV 131
            A DVL A+E+MEF   +  LK +L  +RR   GKK A+ ++K+ K K+  EE      V
Sbjct: 70  NASDVLSAMEEMEFQRFITPLKEALEAYRRDEKGKKEASEQKKKDKDKKDSEEQDKSREV 129

Query: 132 EGTDGESIDGIGNNDNDEEEEEEEEEDEN 157
           E  D E +        DE+++ EEEE +N
Sbjct: 130 EEEDEERL--------DEDDQNEEEEIDN 145

[Close]

BLAST of HG10001257 vs. ExPASy Swiss-Prot
Match: Q642A5 (DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.8e-10
Identity = 48/150 (32.00%), Postives = 82/150 (54.67%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +  +F+ Y ++ +N+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V  LK +L  +RR+  GKK A+ ++K+ K K+  EE      +
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEEQDKSREE 129

Query: 132 GESIDGIGNNDNDEEEEEEEEEDENGASDN 162
                     D DEE  +EEE++E    DN
Sbjct: 130 ---------EDEDEERLDEEEQNEEEEVDN 145

[Close]

BLAST of HG10001257 vs. ExPASy TrEMBL
Match: A0A0A0KEV7 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425700 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 9.5e-65
Identity = 143/161 (88.82%), Postives = 153/161 (95.03%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKK+G G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCES RIFIHYLSAT+N
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASL+EFRRKNAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN 162
           KVEEPTVEGTDGESID +GN+DND + +E+E+EDEN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDND-DNDEDEDEDENRASDD 160

[Close]

BLAST of HG10001257 vs. ExPASy TrEMBL
Match: A0A1S3BNA4 (DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.0e-63
Identity = 142/161 (88.20%), Postives = 148/161 (91.93%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKK+ AG EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCES RIFIHYLSAT+N
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFRRKNAGKKAAA REKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDNDEEEEEEEEEDENGASDN 162
           KVEEPTVEGTDGESIDG+G++DND      E+EDEN ASDN
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDND------EDEDENEASDN 155

[Close]

BLAST of HG10001257 vs. ExPASy TrEMBL
Match: A0A6J1DJA4 (DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.3e-58
Identity = 138/166 (83.13%), Postives = 148/166 (89.16%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKKV A  EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCES RIFIHYLSATSN
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVR LKASLNEFRRK+AGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIDGIGNNDN------DEEEEEEEEEDENGASD 161
           K+EEPTVE    ES D +GN+DN      DE+E E+E+E+ENGASD
Sbjct: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASD 163

[Close]

BLAST of HG10001257 vs. ExPASy TrEMBL
Match: A0A6J1K184 (DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 6.6e-58
Identity = 136/169 (80.47%), Postives = 147/169 (86.98%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKKVGA  EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCES R+FIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIDGIGN--------NDNDEEEEEEEEEDENGASDN 162
             EEPTVEGTDGES D + N        ND DE+E+E+++E ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDKDEVENGGSDD 169

[Close]

BLAST of HG10001257 vs. ExPASy TrEMBL
Match: A0A6J1H495 (DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.3e-57
Identity = 135/165 (81.82%), Postives = 146/165 (88.48%), Query Frame = 0


Query: 1   MEKKVGAGTEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSN 60
           MEKKVGA  EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCES RIFIHYLSATSN
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVR LKASLNEFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIDGIGN----NDNDEEEEEEEEEDENGASDN 162
             EEPTVEGTDGES D + N    ND+  +++E+E+E ENG SD+
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDEDEDEVENGGSDD 165

[Close]

BLAST of HG10001257 vs. TAIR 10
Match: AT2G27470.1 (nuclear factor Y, subunit B11 )

HSP 1 Score: 139.0 bits (349), Expect = 3.2e-33
Identity = 88/174 (50.57%), Postives = 115/174 (66.09%), Query Frame = 0


Query: 10  EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDICKESKRQ 69
           +ELP  IVRRVVK+KLS+CS D D+S++K++LLAF ES RIFIHYLSAT+ND CK+++RQ
Sbjct: 9   DELPLAIVRRVVKKKLSECSPDYDVSIHKEALLAFSESARIFIHYLSATANDFCKDARRQ 68

Query: 70  TIKAEDVLKALEDMEFPELVRSLKASLNEFRRKNAGKKA-------------------AA 129
           T+KA+DV KALE+M+F E +  LK+SL +F++KNAGKKA                    A
Sbjct: 69  TMKADDVFKALEEMDFSEFLEPLKSSLEDFKKKNAGKKAGAAAASYPAGGAALKSSSGTA 128

Query: 130 SREKEAKKKRKVEEPTVEGTDGESI-------DGIGNNDNDEEEEEEEEEDENG 158
           S+ KE KK+++ E  T +G     I       D    NDN EEE   +EEDENG
Sbjct: 129 SKPKETKKRKQEEPSTQKGARKSKIDEETKRNDEETENDNTEEENGNDEEDENG 182

[Close]

BLAST of HG10001257 vs. TAIR 10
Match: AT5G23090.2 (nuclear factor Y, subunit B13 )

HSP 1 Score: 48.9 bits (115), Expect = 4.4e-06
Identity = 31/98 (31.63%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 5   VGAGTEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDI 64
           VG   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFR 101
           C +  ++TI  E VLKAL+ + F E +  + A+  + +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of HG10001257 vs. TAIR 10
Match: AT5G23090.1 (nuclear factor Y, subunit B13 )

HSP 1 Score: 48.9 bits (115), Expect = 4.4e-06
Identity = 31/98 (31.63%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 5   VGAGTEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDI 64
           VG   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SND+
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFR 101
           C +  ++TI  E VLKAL+ + F E +  + A+  + +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of HG10001257 vs. TAIR 10
Match: AT5G08190.1 (nuclear factor Y, subunit B12 )

HSP 1 Score: 48.1 bits (113), Expect = 7.5e-06
Identity = 31/98 (31.63%), Postives = 53/98 (54.08%), Query Frame = 0


Query: 5   VGAGTEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESGRIFIHYLSATSNDI 64
           VG   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ SN++
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PADVRVARDAQDLLIECCVEFINLISSESNEV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRSLKASLNEFR 101
           C +  ++TI  E VLKAL+ + F E V  + A+  + +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

The following BLAST results are available for this feature:

BLAST of HG10001257 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140162.1	2.0e-64	88.82	DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymeras...	[more]
XP_038900596.1	4.4e-64	88.20	DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymer...	[more]
XP_008449581.1	2.2e-63	88.20	PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo]	[more]
KAG7035929.1	3.6e-58	83.64	DNA polymerase epsilon subunit 3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022152961.1	4.7e-58	83.13	DNA polymerase epsilon subunit 3 [Momordica charantia]	[more]

BLAST of HG10001257 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZN5	1.4e-12	34.48	DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1	[more]
Q9NRF9	1.4e-12	34.48	DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1	[more]
Q5R4W3	1.4e-12	34.48	DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1	[more]
Q9JKP7	2.9e-10	32.21	DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1	[more]
Q642A5	3.8e-10	32.00	DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=...	[more]

BLAST of HG10001257 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEV7	9.5e-65	88.82	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4257...	[more]
A0A1S3BNA4	1.0e-63	88.20	DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV...	[more]
A0A6J1DJA4	2.3e-58	83.13	DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 ...	[more]
A0A6J1K184	6.6e-58	80.47	DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=...	[more]
A0A6J1H495	3.3e-57	81.82	DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 P...	[more]

BLAST of HG10001257 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27470.1	3.2e-33	50.57	nuclear factor Y, subunit B11	[more]
AT5G23090.2	4.4e-06	31.63	nuclear factor Y, subunit B13	[more]
AT5G23090.1	4.4e-06	31.63	nuclear factor Y, subunit B13	[more]
AT5G08190.1	7.5e-06	31.63	nuclear factor Y, subunit B12	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 11..80 e-value: 4.3E-8 score: 33.3
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 6..161 e-value: 1.3E-30 score: 108.0
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 12..119
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 102..161
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..161
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 102..128
None	No IPR available	PANTHER	PTHR46172	DNA POLYMERASE EPSILON SUBUNIT 3	coord: 3..154

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001257.1	HG10001257.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006974	cellular response to DNA damage stimulus
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0042766	nucleosome mobilization
cellular_component	GO:0008623	CHRAC
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0031490	chromatin DNA binding
molecular_function	GO:0046982	protein heterodimerization activity