HG10001159 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001159
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA-5-taurinomethyluridine 2-sulfurtransferase
Location	Chr09: 14512105 .. 14517317 (-)
RNA-Seq Expression	HG10001159
Synteny	HG10001159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGAGACTGCGAGTAAAGCCCGTCGTTTGGTGCTCTCTTCCAATCCCTCAGTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGCCACAACCCTCGGCTCTTCCTCACCCAGTTCTCCGCCTTATCTGCCCTGCATAAAACCTCCGAATCTTCATCACCCAAAGGTCTCTCCGATGCCGACTTGGTGCCCTTCCTCTCATGTTCGATGCCTAGCCATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCTTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTGCTTCCTCTTCGTTCTTTTTATGGAATAGATTATATGCTTGTTGTGTTTTCTTGCAAATTAAGGTAGCTGATATTGAAAATGCTGGCGCAATTATGAGTAATCAAATGGTCATTTCAGCACGAAGTATTGTAGCAAAATTAATTGGCGTTTAGGATCATAATTAATGACGAGTGTGGAATTGTTTCTGTTTTTTTTATGCATCATCTGTGTTGGCAGAAGGTAATAGCCTTTTCATGTGACCGTACTATGATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCTTATAAATCCATCTAGACTTAAAAAGTGTAATTGGCCAACGAAGGTCTTAATGAAATAAAGGTTTCCTTGGGAAATTGATCATAGTTCAAGATGTCCATAAGTTATTTCACCATACGGCGCTAAGCGTAATTATAGGTTCTATATAATGGGGAAAATACCTGTCTTATTTTTCTGCCGGAGTTTGTGCTCAAGAGAGATCTTAAGAATTGAAAAAAGTACATTGTTTTTCAGGTTGGTGTACCATTGGAAGTCGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTAAGTGCCTTTACAGTTACAATTGTGTGCTATAACTTTGTTGGAGATCTTAATGATATTAGTACATCCAATCAATTTGGTATAGTTGTGCCAGCTGACCTTCTTGCTCATTCACTCTTTTCTAATTGGATAGGATGAAGTAGTTTTAGGTTCTCTTTTCTGTCTACTGAATATTGCTTAGTTCATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCAGACGTCCTTTGTAACACAAGAATAAAGTTTGGTGTGTTCATTAGATGCACTAGAAAATTTGGCTATATCGTTATGTTATCTTTTACTTCTTTATTTTTTATTTGTATGTTTGTGATTTCTTTTGTATTAATATGATTTAGGACATCCCCTGCTTTTTCCAGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACAAGGTATCTGGAAGTGAAAACGATTGCATGGGCCTTTCTTTCAATCTAACCTATCCAATAGGGCGTATCAGTCATAACTTGCTTAACTTTAATATTTTGTAGTCGATTGTTTTTGTAACACTTTAGGACACTATTTTGTTATTTTCTTGGCCTTTTTTATATTCTACGCATGACAGCTAGTGATGTCTTTAGTAATTCAACTTGAAGTCTCATGTATAAACTCAGCAGAACAAATATGACCTATATTGAATTACATGGGCCAATATTTGTTGTAATCCTTCTAGAAACAACATCATTAATAAGTACTATGTATTGCTTACCTAGTTCAAGGTTTATCGTCAAACACATTTAACCCTTTGTTGTTCCTTCTTGCTCCAAACTATAGATTTCTTATTTTACTCCACTATTATTGTTTTTTAGGTCAAGGATCAAACTTATTTTCTATCACATCTCTCACAGAATCAGCTTAAACGGCTTTTATTTCCTCTTGGTTGTATTCCGAAGGTTTGTTCAAGCTTACTTTACAGCATTTATTATCATGTATTTTTTAAGATATCACTTATGTTTCAGACTTCTGAGTGTTATCCGAGAGGTTCAGCTATTGTAAATTTGTATGATTTGGTACTGGCCTTCTAAGCTTCTACAGACATAAACACCTAGCGGCTAACTTGGTTGCATTCAGTTGAGGAATTTATGCATTTAATAAAAGTTAAAAATTGTATTGGACTTTGGTAAACCAATTGGTGTAGATATCATATTAGAGCTTATTGGATGTTATCCTGCTGTCTGAAGCTATTAAGTTTTTAACTACTCTTGTGTGCCAAACTTATATAAGTGCTTCAATTTGTAAGTTCATATCATGAGTTAGCACTTGCTCCTATCTCAACTAGCTACATTCTTTCAACTAATGCACCATCTTTTTTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATTTTGTTAATATTTTTCTAGAGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGACGAGGTTCGCAAACTTGCTGCAAAGTTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTCCTTGGGAAGGTACCTTTGGATGTGGTGATTGAAACATCTGTTTACTTGGAGAACTTGCAAAAACCATTCCTCAATGCCAGTATTTCTAACTATTTGGTGTTGCATCTTAAGAATATTCTATTACTTGTTATAACTATTGAATATGATTGAGGCTAGCTTAAAGAATTTAGTATAAGATTTGCTAACTCTTGAAATTTTCTGATGGCTAAACTATCCCTGAGCTAGTTTTATATTTCCCTATTCTTCCTGACAGCAAAAATTTTCATTCATATAGTTTATGAAGATGGCGCTCATGTAAGGTATCTTGTGGGTATAATTCATGTAGAACCTGTTTCTTTGATTTGTAGATAAAATTTAGTGAGTTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTTTCAGTTTCTTTTCATTAAGAATCAATTCAATGTTCTCACAAGTTCACATTCTTTACTTACGTCTCTTGCTGAGCTATATTACAGTGACGATTTTAGCTTTTATGCCTTGGTCTAATGCAGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCAAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTTTGCCCCCAGACCAGTTTACTGGGCTTCAATGTAAGGTAAAGTTTCATCTTCCATCTCCCATATTTCTCGTTAGTTTCAGTACTTCAAGGTTGGCCTGGCGGTAACGGTATAACAGAGAAGGCAATTGTCTTAACATTTTATTCAAACCTTTGCTGAGAAGCCATCAGTTTATTCCTGCAAGTTTTTGTTGGAAGTTTGAACAAGCATGCTTGGGAAAATTGATAATCCGGAAATTTTGACTATTACGTAAAAAATTGGATCTTCCATGATTATATAATAGAACAAGCCAAACAAAGAAGACAGGTTGGTTGACCATTCTGCATGCCTTCTTCTAGACATTATGGGGCGAGCGTAATAAATGCATTTTTAGAGATTCCTTTTTATTTATTTTTTAATACTTTCATGGATTTAGTTTTGTCTACTGCGTTATATTGGTGCAAAACTAGGCACTTTTTTAAGAATTTTAGTCATTTTTATTTAGTTTCCAATTGAAAATATCTTATGTAATCACCTTTAGGTGCTTGGAGTCTCTCCTTATTTCATTTACTCAATGAAATGTTTCTTCTCTAAAAAAAAATTGATAATCCAGAAATGTTGACTATTACGTAAAAATTCGATCTTCCATGATTATGTAATAGAACAAGCATCTTGACATCATGGGCTTTTCTTTCAAAGAAAGGATGTAAGTTTAGAATTCAATTTTATTTGCTAAGGGACTGTTTAGGGGGCCTTTCTTTTCTAGATGGGAAACGTATTAGAAATAGTATGGGGATGCTATGTGTCTTGGGCATGCTTGTCCAGGGCGTATTTGCCTATGGCCGCATGCCCAAACCATCCTCAACCATGTCATATGTATGTCTTACTATTAATTTATCAAGTTGCTTTGTTGTATGTTGTGTTTTTATTTTTCTTGCGACGAGGTAGCATTTCCAGCGTGCAAGCTTGCCAGTCTAGTTTTTGCTTAGAATGTACTATAGGGGGAACACACCTGTCATCCCCCGACCAAGTATTGTTGTACTCTTTGCAATCAAAGCTTTTCCTTGCAATTGTCAGTCTTCAATGCTTTCTTTAAAGATGTTTTCAATGTTATTTCTCTCTCCCGTTTTATAAAACCATTTTCTCTTGAAACGGGCGGGGAGGCTAGACCCTATAGCGAGATTGTCCGCGATTTCTGGAATTGTACGGCTGACTTGCTTGCCGGGTTAGAGCAAGTGCCGCACAATCGCTCGTCTCGTATTTGAAAAGTTGCGATTGTGACACTATGCCTGCTATATACCGCTCATTTGCTTTGAGATTGTGTTATCGGACTCTAGCTTCTTATCATGTGATTTCCTGTTTCCCATTGTTTCATGATTTTCTACAAATTTTTAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACGATGAGGATGGTCATGAAGATATTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACGAATGGAAGATAAATCGAAGCTCGGTAAACCAATAACGATAAAAGTGAAACCAGAGAGTGATCTTAATGAATCTGAAGCTAAAGATGATACAAAAATACACGGCGAGTTAAGAAATCAAGAAACCACTGTGGTTGAGCAAGTAAGAGAAGTTTCTTCAGATGAAGCAGTTGCCACAGCCCCAGTGAACTGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGATATTTTAG

mRNA sequence

ATGCTGAGACTGCGAGTAAAGCCCGTCGTTTGGTGCTCTCTTCCAATCCCTCAGTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGCCACAACCCTCGGCTCTTCCTCACCCAGTTCTCCGCCTTATCTGCCCTGCATAAAACCTCCGAATCTTCATCACCCAAAGGTCTCTCCGATGCCGACTTGGTGCCCTTCCTCTCATGTTCGATGCCTAGCCATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCTTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGGTGTACCATTGGAAGTCGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCAGACGTCCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACAAGGTCAAGGATCAAACTTATTTTCTATCACATCTCTCACAGAATCAGCTTAAACGGCTTTTATTTCCTCTTGGTTGTATTCCGAAGGACGAGGTTCGCAAACTTGCTGCAAAGTTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTCCTTGGGAAGATAAAATTTAGTGAGTTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCAAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTTTGCCCCCAGACCAGTTTACTGGGCTTCAATGTAAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACGATGAGGATGGTCATGAAGATATTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACGAATGGAAGATAAATCGAAGCTCGGTAAACCAATAACGATAAAAGTGAAACCAGAGAGTGATCTTAATGAATCTGAAGCTAAAGATGATACAAAAATACACGGCGAGTTAAGAAATCAAGAAACCACTGTGGTTGAGCAAGTAAGAGAAGTTTCTTCAGATGAAGCAGTTGCCACAGCCCCAGTGAACTGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGATATTTTAG

Coding sequence (CDS)

ATGCTGAGACTGCGAGTAAAGCCCGTCGTTTGGTGCTCTCTTCCAATCCCTCAGTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGCCACAACCCTCGGCTCTTCCTCACCCAGTTCTCCGCCTTATCTGCCCTGCATAAAACCTCCGAATCTTCATCACCCAAAGGTCTCTCCGATGCCGACTTGGTGCCCTTCCTCTCATGTTCGATGCCTAGCCATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCTTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGGTGTACCATTGGAAGTCGTTCATTTGACAGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCAGACGTCCTTTGTAACACAAGAATAAAGTTTGGTGCATTCATGGATGCAATAAGCAATATGGAGTTTGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCACCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACAAGGTCAAGGATCAAACTTATTTTCTATCACATCTCTCACAGAATCAGCTTAAACGGCTTTTATTTCCTCTTGGTTGTATTCCGAAGGACGAGGTTCGCAAACTTGCTGCAAAGTTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTCCTTGGGAAGATAAAATTTAGTGAGTTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCAAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTTTGCCCCCAGACCAGTTTACTGGGCTTCAATGTAAGGTCCGACACGGCCCGAGTTTCTATAGTTGCAATTTGACAATGGAACACGATGAGGATGGTCATGAAGATATTGCAGTTGTCCACCTAAGCGAAGACGACCAAGGCCTTGCAGCAGGCCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACGAATGGAAGATAAATCGAAGCTCGGTAAACCAATAACGATAAAAGTGAAACCAGAGAGTGATCTTAATGAATCTGAAGCTAAAGATGATACAAAAATACACGGCGAGTTAAGAAATCAAGAAACCACTGTGGTTGAGCAAGTAAGAGAAGTTTCTTCAGATGAAGCAGTTGCCACAGCCCCAGTGAACTGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSPKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF

Homology

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BLAST of HG10001159 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 1046.6 bits (2705), Expect = 7.4e-302
Identity = 508/537 (94.60%), Postives = 520/537 (96.83%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSP 60
           MLRLRVKPVVWCSLPI  FTSLPLSPSILRPVLRR NPRLFLT+FSA SAL KT ESSSP
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60

Query: 61  KGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSDADLVPFLSCSMPS+PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQV VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360

Query: 361 RRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQ 420
           RRVFRVGSLKWLTG+PPDQF+GLQCKVRHGPSFYSCNLTMEH+EDG EDIAVVHLSEDDQ
Sbjct: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPE 480
           GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENA+MEDKSKLGKPI IKVKPE
Sbjct: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           S L ESEAKDDTKIHGE  NQETTV++Q+ EVSSDEA+ TAP+ WLGSLRKKLLQ+F
Sbjct: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

[Close]

BLAST of HG10001159 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1017.3 bits (2629), Expect = 4.8e-293
Identity = 490/537 (91.25%), Postives = 511/537 (95.16%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSP 60
           MLRLRV P VWCS+PI  FTSLPL+PSILRP+LRRHNPRLFLT+FSA SA+H TS SSS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQV VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQ 420
           RRVFRVGSLKWL+G PPDQFT LQCKVRHGPSFYSCNLTMEH EDGHED+AVVHLSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPE 480
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 SDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           S + ES+AKDDT+IH +LRN+E TVVEQ+REVS DEA+AT  + WLGSL+KKLLQIF
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of HG10001159 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1014.6 bits (2622), Expect = 3.1e-292
Identity = 488/538 (90.71%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSAL-HKTSESSS 60
           MLRLRV P VWCS+PI  FTSLPLSPSILRP+LR HNPRLF T+FSA SA+ H TS+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+ VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDD 420
           RRRVFRVGSLKWL+G+PPDQFTGLQCKVRHGP FYSCNLTMEH+EDGHED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +S +NES+AKDDTKI  E RN+E TVVEQ+R+VS DEA+AT P+ WLGSLRKKLLQIF
Sbjct: 482 DSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of HG10001159 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.7e-291
Identity = 485/538 (90.15%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSAL-HKTSESSS 60
           MLRLRV P VWCS+PI  FTSLPLSPSILRP+LR HNPRLF T+FSA SA+ H TS+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+ VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDD 420
           RRRVFRVGSLKWL+G+PPD+FTGLQCKVRHGP FYSCNLTMEH++DGHED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +S +NES+AKDDTKI  + RN+E TVVEQ+R+VS DEA+AT P+ WLGSLRKKLLQIF
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of HG10001159 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 996.5 bits (2575), Expect = 8.8e-287
Identity = 484/537 (90.13%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSP 60
           MLRLRV P VWC+LPI  FTSLPL PSILRPVLRRH PRLFLT+FSA SA+HK SESSS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 61  KGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSD DL PFLSCSMP HPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQV VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKD VKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 361 RRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQ 420
           RR+FRVGSLKWL+GL PDQFT LQCKVRHGPSFYSCNLTME +ED  ED+AVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPE 480
           GLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E A+MEDKSKLGKPI IKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +   E E KDDTKIHG L+NQ+TTVVEQ  EV+SDEA+ TAP+ WLGSLRKKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of HG10001159 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.8e-101
Identity = 171/365 (46.85%), Postives = 248/365 (67.95%), Query Frame = 0


Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 141 CDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C +  VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        DK   L+ +KDK+KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+ G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 381 FTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of HG10001159 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.5e-100
Identity = 168/365 (46.03%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 141 CDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++  VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +   +L+ +KDK+KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+   P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 381 FTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  +     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of HG10001159 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.5e-100
Identity = 168/365 (46.03%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 141 CDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++  VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +   +L+ +KDK+KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+   P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 381 FTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  +     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of HG10001159 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.0e-100
Identity = 167/365 (45.75%), Postives = 249/365 (68.22%), Query Frame = 0


Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 141 CDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++  +P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +K   L+ +KDK+KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+ G+P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 381 FTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  +     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]

BLAST of HG10001159 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 355.5 bits (911), Expect = 1.0e-96
Identity = 168/366 (45.90%), Postives = 243/366 (66.39%), Query Frame = 0


Query: 81  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 141 CDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C +  VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            + +GHYA +     +  +   +L+ +KDK+KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGLPPDQ 380
           F+TIGQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W+    P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 381 FTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
              L  K+RHG     C + M  +     DI  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 441 GVILES 446
             IL++
Sbjct: 361 AQILQT 352

[Close]

BLAST of HG10001159 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.3e-293
Identity = 490/537 (91.25%), Postives = 511/537 (95.16%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSP 60
           MLRLRV P VWCS+PI  FTSLPL+PSILRP+LRRHNPRLFLT+FSA SA+H TS SSS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQV VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQ 420
           RRVFRVGSLKWL+G PPDQFT LQCKVRHGPSFYSCNLTMEH EDGHED+AVVHLSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPE 480
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 SDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           S + ES+AKDDT+IH +LRN+E TVVEQ+REVS DEA+AT  + WLGSL+KKLLQIF
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]

BLAST of HG10001159 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.5e-292
Identity = 488/538 (90.71%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSAL-HKTSESSS 60
           MLRLRV P VWCS+PI  FTSLPLSPSILRP+LR HNPRLF T+FSA SA+ H TS+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+ VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDD 420
           RRRVFRVGSLKWL+G+PPDQFTGLQCKVRHGP FYSCNLTMEH+EDGHED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +S +NES+AKDDTKI  E RN+E TVVEQ+R+VS DEA+AT P+ WLGSLRKKLLQIF
Sbjct: 482 DSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of HG10001159 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.7e-291
Identity = 485/538 (90.15%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSAL-HKTSESSS 60
           MLRLRV P VWCS+PI  FTSLPLSPSILRP+LR HNPRLF T+FSA SA+ H TS+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+ VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDD 420
           RRRVFRVGSLKWL+G+PPD+FTGLQCKVRHGP FYSCNLTMEH++DGHED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +S +NES+AKDDTKI  + RN+E TVVEQ+R+VS DEA+AT P+ WLGSLRKKLLQIF
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of HG10001159 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.7e-291
Identity = 485/538 (90.15%), Postives = 512/538 (95.17%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSAL-HKTSESSS 60
           MLRLRV P VWCS+PI  FTSLPLSPSILRP+LR HNPRLF T+FSA SA+ H TS+SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP++PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+ VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKD VKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDD 420
           RRRVFRVGSLKWL+G+PPD+FTGLQCKVRHGP FYSCNLTMEH++DGHED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENA+MEDKSKLGKPI IKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +S +NES+AKDDTKI  + RN+E TVVEQ+R+VS DEA+AT P+ WLGSLRKKLLQIF
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]

BLAST of HG10001159 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.3e-287
Identity = 484/537 (90.13%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 1   MLRLRVKPVVWCSLPIPQFTSLPLSPSILRPVLRRHNPRLFLTQFSALSALHKTSESSSP 60
           MLRLRV P VWC+LPI  FTSLPL PSILRPVLRRH PRLFLT+FSA SA+HK SESSS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 61  KGLSDADLVPFLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSD DL PFLSCSMP HPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQV VPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKD VKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 361 RRVFRVGSLKWLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQ 420
           RR+FRVGSLKWL+GL PDQFT LQCKVRHGPSFYSCNLTME +ED  ED+AVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKPE 480
           GLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E A+MEDKSKLGKPI IKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SDLNESEAKDDTKIHGELRNQETTVVEQVREVSSDEAVATAPVNWLGSLRKKLLQIF 538
           +   E E KDDTKIHG L+NQ+TTVVEQ  EV+SDEA+ TAP+ WLGSLRKKLLQ+F
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]

BLAST of HG10001159 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 704.5 bits (1817), Expect = 6.5e-203
Identity = 334/424 (78.77%), Postives = 367/424 (86.56%), Query Frame = 0


Query: 71  FLSCSMPSHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWD 130
           +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++CPW+
Sbjct: 72  YLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWE 131

Query: 131 DDLKYAKAVCDQVGVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 190
           DDLKYAK VC+QV VPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM
Sbjct: 132 DDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 191

Query: 191 DAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDKVKDQTYFLSHLSQNQLKRLLF 250
           DAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+D VKDQTYFLSHLSQ QLKRLLF
Sbjct: 192 DAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLLF 251

Query: 251 PLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGD 310
           PLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAESGD
Sbjct: 252 PLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAESGD 311

Query: 311 FLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLK 370
           FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR+FRVGSL+
Sbjct: 312 FLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLR 371

Query: 371 WLTGLPPDQFTGLQCKVRHGPSFYSCNLTMEHDEDGHEDIAVVHLSEDDQGLAAGQFAAF 430
           W +G P  +   L+CKVRHGP FYSC+  ME    G  D+AVVHL EDDQGLAAGQFAAF
Sbjct: 372 WNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQFAAF 431

Query: 431 YKGRRCIGSGVILESWDDQGFPVCPKAVENARMEDKSKLGKPITIKVKP-----ESDLNE 490
           Y+G  CIGSGVILESWDDQ FPVC KA++ A +EDK+KLGKP+ I   P     E+D  E
Sbjct: 432 YEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMPTMSSVEADTGE 491

The following BLAST results are available for this feature:

BLAST of HG10001159 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901654.1	7.4e-302	94.60	tRNA-specific 2-thiouridylase MnmA [Benincasa hispida]	[more]
XP_004142116.1	4.8e-293	91.25	uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy...	[more]
KAA0041410.1	3.1e-292	90.71	tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa]	[more]
XP_008449720.1	7.7e-291	90.15	PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp...	[more]
XP_022944568.1	8.8e-287	90.13	uncharacterized protein LOC111448983 [Cucurbita moschata]	[more]

BLAST of HG10001159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0SMH1	1.8e-101	46.85	tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN...	[more]
O51625	1.5e-100	46.03	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ...	[more]
B7J2N7	1.5e-100	46.03	tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598...	[more]
Q660I8	2.0e-100	45.75	tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496...	[more]
B2S125	1.0e-96	45.90	tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314...	[more]

BLAST of HG10001159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX16	2.3e-293	91.25	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7TDN6	1.5e-292	90.71	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3B8G8	3.7e-291	90.15	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3BNL8	3.7e-291	90.15	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1FY63	4.3e-287	90.13	tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of HG10001159 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51310.1	6.5e-203	78.77	transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023382	Adenine nucleotide alpha hydrolase-like domains	GENE3D	2.30.30.280		coord: 295..354 e-value: 7.0E-19 score: 69.0
None	No IPR available	PFAM	PF03054	tRNA_Me_trans	coord: 82..442 e-value: 8.1E-127 score: 422.9
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 361..450 e-value: 9.9E-14 score: 52.7
None	No IPR available	PANTHER	PTHR43052	FAMILY NOT NAMED	coord: 65..475
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 82..327
IPR004506	tRNA-specific 2-thiouridylase	TIGRFAM	TIGR00420	TIGR00420	coord: 82..442 e-value: 4.2E-111 score: 369.2
IPR004506	tRNA-specific 2-thiouridylase	HAMAP	MF_00144	tRNA_thiouridyl_MnmA	coord: 81..443 score: 33.535908
IPR004506	tRNA-specific 2-thiouridylase	CDD	cd01998	tRNA_Me_trans	coord: 82..442 e-value: 6.14276E-162 score: 462.376
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 72..294 e-value: 5.0E-86 score: 289.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001159.1	HG10001159.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061708	tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0016740	transferase activity