HG10001069 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001069
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Ubiquitin-conjugating enzyme family protein
Location	Chr09: 13725573 .. 13728026 (+)
RNA-Seq Expression	HG10001069
Synteny	HG10001069

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGGAAGTCGAGACCACCGGCCGCCACTGCCTCTCTTTGGTGGGGGGAGATCGTCTTCTTCAAACTCCTTAGCACCTCCAACTTCATGGGCGTCAACAACCTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGACTGAGTTTAACTTGGACCCACCTTCCGATTGCTCCGCCGGCCCTAAGGGTGACAATCTTTACCACTGGTTCGCTACCATTTTTGGCCCTCCAGGTAGCTGCCACCCTTCTCTTTCTTTACTTTTTGGGGTTTATCATTTCTCTACTCGTACGGTTTTTGGTTTCTTGTTCTTCTTTTACTCCCTCGCTTTGCCCGATGATTTCTTGTTTGTAGATGAGACATTCACTTTTCAGCCAGATTTTTATGATAAACATCGCTTTAATTGGCTATGGCTGTTAGGGTTCCACTTTCCTGCTGCTTTATTATTATTTTTTTTTAAAAGAAGCTTTTTATCATCTTCGGTGCAAGAGTTCTCGTTCAGTGAGCTATTACACTCTCTTTCTTTGTTAGAAATTCTTTAGTTCTGGGGCTGGATTTTTAGCCATCTAAAGATATGTTGCTTTTGTTTACAATTCCTGAACAGATGCCTCACTGAAACTCTAATGGGCTCTTATTACCTGATTGCTAAACATATTCTTTGCCTTCAACTACCAAAGGTTTAGTTACAATTCTTAAGAAAGTGATTAGGGGAGAAGATGTTCACTATTCAGAGGAGTGTATATACTTGGTGTGCAATGCACCTCTGTCTCTGTGTGTTTTGTGCAAACAATAAAGATTGGATTAGTTGCCAAGTAAATGGCGTAAGAGTTGGAGAGAAACTACATTTAGATTAAAGTTGTCTTACCAAAAAGAAATTTTAGATAAAGTTGTTGAACACACAATCTTCCAGATGAAATTTGAGCAAGAAACTGAAATATAATCCAAAAATACACAAGGTGCTGCCATGGACTTCCTACCAAGTTACATGCTCAAACCCAATTTACATCTATATATCTGATATCGTCCAAAAATACATAAAATGATTAGAGGAGATGCTCACTGATGGAGTTCGATTACCCGGCCACTTGATATCAAAAACTCCGAAGGATTTGGAAGAATGTCCTCTAGCTATATAGCCACATGTCACTGTTGTATACACATGCTTAAATGAATGAAATGAGAAATGAGATCCGTGTCAGGATTTCAACTAGTGTTTTAGACAATAAACGTTTTTTATTATATGAGGGCTTTGTATGAATACTATCTCCAATAAATCAGGAAAACTTACTGATCAATTTCATCTAGCCTTTTCCGACCGAAGTAATTTTGACAAATATATTTGTCTTGTTTTTAGGAACACCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTACTCTTGCTCTTATCCTTAAATTTTAGGATACAAAAACTAAATTCTCTTACCCTCATCATGAGAATCGGATGATCATTCTTGACCTGATGCTACACTGTTCTGAGTTTTATACTCATGTTTATTTATTAATAAGGATTGGGTTTGCAGGTTGTATTTAAAACCCGTATCTACCACTGCAATGTCGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCCGCGTTAACCATCGCAAAGGTGTTAATTGCGGTGAAATCAATTTTTGCAAATCCCAACCCTTGTGAGTTACCTCACTTATTTGGACTCATTATTTATGTCACTTTAGACTTGCTTTAGTTGAGTTCATATGCCTTTCTATTTCAAGAGCTTCTTTAAAGCTAAACCTGCCTTTTTTTCATTTGGTTCTTGTAAATTGAGAAATAGGTCATTGTTGTAAGCAAAAACAAACCAAAAATAAAATAAAAATTGAGACATAGATAATTCTTTGCTTCACACTGCATTGAGTTCTTTAACATCAGTTACAATTGCAATCCACCTAAAGTTGCAGAGGGGAGTTTCTACTTTTTATCAATATTAAAGATAAATTGTGACCAATTAGCTTGTCTTTTGTGCGTCTTTAAGCGCTGGGAGTGTAAAATGAAAGGATACAGATGCTTGGTAAGGTTGGGTTTTCAAAGGGGGAGGGATTCAGTGATTGTTGGAAGGAATTTACTGGGGAGTACAAACTTGGGGGGGTGGGGGAGGTCCTGATCGGAGAGATGGCTGGTCCGTTTTCCACGAAAATATAACGGGAATGAAAAGACAGACGTAGAATGTCAATGTGCTAACTTTTCTATACAACTAATGGATCTAGTTGATGGCAACTCTGAAATCCCACATTCATTTTGGATTTTACTAATTTTGCAGATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGGAAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTTGCTAAGTGA

mRNA sequence

ATGGTGGGAAGTCGAGACCACCGGCCGCCACTGCCTCTCTTTGGTGGGGGGAGATCGTCTTCTTCAAACTCCTTAGCACCTCCAACTTCATGGGCGTCAACAACCTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGACTGAGTTTAACTTGGACCCACCTTCCGATTGCTCCGCCGGCCCTAAGGGTGACAATCTTTACCACTGGTTCGCTACCATTTTTGGCCCTCCAGGAACACCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTTGTATTTAAAACCCGTATCTACCACTGCAATGTCGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCCGCGTTAACCATCGCAAAGGTGTTAATTGCGGTGAAATCAATTTTTGCAAATCCCAACCCTTATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGGAAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTTGCTAAGTGA

Coding sequence (CDS)

ATGGTGGGAAGTCGAGACCACCGGCCGCCACTGCCTCTCTTTGGTGGGGGGAGATCGTCTTCTTCAAACTCCTTAGCACCTCCAACTTCATGGGCGTCAACAACCTCGGTTTCTGCTTCAGGCAAGAGAATTCAAAGAGAAATGACTGAGTTTAACTTGGACCCACCTTCCGATTGCTCCGCCGGCCCTAAGGGTGACAATCTTTACCACTGGTTCGCTACCATTTTTGGCCCTCCAGGAACACCATATGAAGGTGGCATATTTTTCCTGGACATAATTTTTCCAAGTGATTATCCATTTAATCCTCCAAAGGTTGTATTTAAAACCCGTATCTACCACTGCAATGTCGATTCCACTGGTAATGTAAGTTTGGACATTTTGAAGGATAACTGGAGTCCCGCGTTAACCATCGCAAAGGTGTTAATTGCGGTGAAATCAATTTTTGCAAATCCCAACCCTTATGATCCAAATGCACCTGGTGTTGCTCACTTGTACTTGGCAGACAGAGGAAAACACGACGAGATAGCTGCTGAGTGGACGCTACGTTTTGCTAAGTGA

Protein sequence

MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTLRFAK

Homology

[Close]

BLAST of HG10001069 vs. NCBI nr
Match: XP_038902201.1 (constitutive photomorphogenesis protein 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 377.9 bits (969), Expect = 5.1e-101
Identity = 179/185 (96.76%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP LPLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPQLPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS G
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           N SLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NASLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. NCBI nr
Match: XP_038902200.1 (constitutive photomorphogenesis protein 10 isoform X1 [Benincasa hispida])

HSP 1 Score: 375.9 bits (964), Expect = 1.9e-100
Identity = 178/184 (96.74%), Postives = 179/184 (97.28%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP LPLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPQLPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS G
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSAG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           N SLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NASLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFA 185
           LRFA
Sbjct: 181 LRFA 184

[Close]

BLAST of HG10001069 vs. NCBI nr
Match: XP_022943567.1 (constitutive photomorphogenesis protein 10 [Cucurbita moschata] >XP_022986179.1 constitutive photomorphogenesis protein 10 [Cucurbita maxima] >XP_023512726.1 constitutive photomorphogenesis protein 10 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7010225.1 Constitutive photomorphogenesis protein 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.0 bits (959), Expect = 7.4e-100
Identity = 177/185 (95.68%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP  PLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKD+WSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

[Close]

BLAST of HG10001069 vs. NCBI nr
Match: KAG6570333.1 (Constitutive photomorphogenesis protein 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 374.0 bits (959), Expect = 7.4e-100
Identity = 177/185 (95.68%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP  PLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS+G
Sbjct: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKD+WSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. NCBI nr
Match: XP_004142171.1 (constitutive photomorphogenesis protein 10 [Cucumis sativus] >KGN54065.1 hypothetical protein Csa_018040 [Cucumis sativus])

HSP 1 Score: 372.1 bits (954), Expect = 2.8e-99
Identity = 178/185 (96.22%), Postives = 179/185 (96.76%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRPPLP  GGGRSSSS SLA PTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. ExPASy Swiss-Prot
Match: Q9LJD7 (Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana OX=3702 GN=COP10 PE=1 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 1.8e-69
Identity = 126/184 (68.48%), Postives = 144/184 (78.26%), Query Frame = 0


Query: 3   GSRDHRP-PLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSA 62
           GS   RP P  ++ G   ++S       SW + TSVSASGKRIQREM E N+DPP DCSA
Sbjct: 6   GSGRLRPLPTAMYAGYSGTAS-------SWVAKTSVSASGKRIQREMAELNIDPPPDCSA 65

Query: 63  GPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGN 122
           GPKGDNLYHW ATI GP GTPYEGGIFFLDIIFPSDYPF PPK+VFKTRIYHCNVD+ G+
Sbjct: 66  GPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGD 125

Query: 123 VSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTL 182
           +S++IL+D+WSPALTI KVL A++SIF  P PY P  P +A LYL DR KHDE+A EWTL
Sbjct: 126 LSVNILRDSWSPALTITKVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKEWTL 182

Query: 183 RFAK 186
           RFAK
Sbjct: 186 RFAK 182

[Close]

BLAST of HG10001069 vs. ExPASy Swiss-Prot
Match: Q2T9X7 (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus OX=9913 GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.9e-53
Identity = 101/182 (55.49%), Postives = 126/182 (69.23%), Query Frame = 0


Query: 5   RDHRPPLPLFGGGRSSSSNSLAPPTSWAS--TTSVSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S  T  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GPPG+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ S+  + NP DP    +A  YL +R +HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

[Close]

BLAST of HG10001069 vs. ExPASy Swiss-Prot
Match: Q969T4 (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens OX=9606 GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 1.9e-53
Identity = 101/182 (55.49%), Postives = 126/182 (69.23%), Query Frame = 0


Query: 5   RDHRPPLPLFGGGRSSSSNSLAPPTSWAS--TTSVSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S  T  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GPPG+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ S+  + NP DP    +A  YL +R +HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

[Close]

BLAST of HG10001069 vs. ExPASy Swiss-Prot
Match: P52483 (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus OX=10090 GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 210.3 bits (534), Expect = 1.9e-53
Identity = 101/182 (55.49%), Postives = 126/182 (69.23%), Query Frame = 0


Query: 5   RDHRPPLPLFGGGRSSSSNSLAPPTSWAS--TTSVSASGKRIQREMTEFNLDPPSDCSAG 64
           RD   P P     R  S+      T  +S  T  +S S KRIQ+E+ E  LDPP +CSAG
Sbjct: 25  RDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAG 84

Query: 65  PKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGNV 124
           PKGDN+Y W +TI GPPG+ YEGG+FFLDI F SDYPF PPKV F+TRIYHCN++S G +
Sbjct: 85  PKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVI 144

Query: 125 SLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTLR 184
            LDILKDNWSPALTI+KVL+++ S+  + NP DP    +A  YL +R +HD IA +WT R
Sbjct: 145 CLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKR 204

[Close]

BLAST of HG10001069 vs. ExPASy Swiss-Prot
Match: Q96LR5 (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens OX=9606 GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.5e-52
Identity = 89/152 (58.55%), Postives = 115/152 (75.66%), Query Frame = 0


Query: 33  STTSVSASGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDI 92
           +   +S S KRIQ+E+ E  LDPP +CSAGPKGDN+Y W +TI GPPG+ YEGG+FFLDI
Sbjct: 49  TAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDI 108

Query: 93  IFPSDYPFNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKSIFANPN 152
            F  DYPF PPKV F+TRIYHCN++S G + LDILKDNWSPALTI+KVL+++ S+  + N
Sbjct: 109 TFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCN 168

Query: 153 PYDPNAPGVAHLYLADRGKHDEIAAEWTLRFA 185
           P DP    +A  Y+ +R +HD +A +WT R+A
Sbjct: 169 PADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200

[Close]

BLAST of HG10001069 vs. ExPASy TrEMBL
Match: A0A6J1JFC7 (constitutive photomorphogenesis protein 10 OS=Cucurbita maxima OX=3661 GN=LOC111483999 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 3.6e-100
Identity = 177/185 (95.68%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP  PLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKD+WSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

[Close]

BLAST of HG10001069 vs. ExPASy TrEMBL
Match: A0A6J1FTD1 (constitutive photomorphogenesis protein 10 OS=Cucurbita moschata OX=3662 GN=LOC111448300 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 3.6e-100
Identity = 177/185 (95.68%), Postives = 180/185 (97.30%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRP  PLFGGGRSSSS SLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 12  MVGNRDHRPQQPLFGGGRSSSSTSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 71

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDS+G
Sbjct: 72  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSSG 131

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKD+WSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 132 NVSLDILKDSWSPALTIAKVLIAVKSIFTNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 191

Query: 181 LRFAK 186
           LRFAK
Sbjct: 192 LRFAK 196

[Close]

BLAST of HG10001069 vs. ExPASy TrEMBL
Match: A0A0A0KWU2 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280420 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.4e-99
Identity = 178/185 (96.22%), Postives = 179/185 (96.76%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RDHRPPLP  GGGRSSSS SLA PTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDHRPPLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. ExPASy TrEMBL
Match: A0A5D3DDP3 (Constitutive photomorphogenesis protein 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00610 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.8e-97
Identity = 175/185 (94.59%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RD RP LP  GGGRSSSS SLA PTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDQRPQLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFVNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. ExPASy TrEMBL
Match: A0A1S3BMW7 (constitutive photomorphogenesis protein 10 OS=Cucumis melo OX=3656 GN=LOC103491591 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.8e-97
Identity = 175/185 (94.59%), Postives = 176/185 (95.14%), Query Frame = 0


Query: 1   MVGSRDHRPPLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60
           MVG+RD RP LP  GGGRSSSS SLA PTSWASTTSVSASGKRIQREMTEFNLDPPSDCS
Sbjct: 1   MVGNRDQRPQLPPLGGGRSSSSISLAAPTSWASTTSVSASGKRIQREMTEFNLDPPSDCS 60

Query: 61  AGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120
           AGPKGDNLYHWFATIFGP GTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG
Sbjct: 61  AGPKGDNLYHWFATIFGPSGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTG 120

Query: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWT 180
           NVSLDILKDNWSPALTIAKVLIAVKSIF NPNPYDPNAPGVAHLYLADR KHDEIAAEWT
Sbjct: 121 NVSLDILKDNWSPALTIAKVLIAVKSIFVNPNPYDPNAPGVAHLYLADRAKHDEIAAEWT 180

Query: 181 LRFAK 186
           LRFAK
Sbjct: 181 LRFAK 185

[Close]

BLAST of HG10001069 vs. TAIR 10
Match: AT3G13550.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 263.5 bits (672), Expect = 1.3e-70
Identity = 126/184 (68.48%), Postives = 144/184 (78.26%), Query Frame = 0


Query: 3   GSRDHRP-PLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSA 62
           GS   RP P  ++ G   ++S       SW + TSVSASGKRIQREM E N+DPP DCSA
Sbjct: 6   GSGRLRPLPTAMYAGYSGTAS-------SWVAKTSVSASGKRIQREMAELNIDPPPDCSA 65

Query: 63  GPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGN 122
           GPKGDNLYHW ATI GP GTPYEGGIFFLDIIFPSDYPF PPK+VFKTRIYHCNVD+ G+
Sbjct: 66  GPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGD 125

Query: 123 VSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGVAHLYLADRGKHDEIAAEWTL 182
           +S++IL+D+WSPALTI KVL A++SIF  P PY P  P +A LYL DR KHDE+A EWTL
Sbjct: 126 LSVNILRDSWSPALTITKVLQAIRSIFLKPEPYSPALPVIARLYLTDREKHDEVAKEWTL 182

Query: 183 RFAK 186
           RFAK
Sbjct: 186 RFAK 182

[Close]

BLAST of HG10001069 vs. TAIR 10
Match: AT3G13550.2 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 215.7 bits (548), Expect = 3.1e-56
Identity = 104/152 (68.42%), Postives = 120/152 (78.95%), Query Frame = 0


Query: 3   GSRDHRP-PLPLFGGGRSSSSNSLAPPTSWASTTSVSASGKRIQREMTEFNLDPPSDCSA 62
           GS   RP P  ++ G   ++S       SW + TSVSASGKRIQREM E N+DPP DCSA
Sbjct: 6   GSGRLRPLPTAMYAGYSGTAS-------SWVAKTSVSASGKRIQREMAELNIDPPPDCSA 65

Query: 63  GPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFNPPKVVFKTRIYHCNVDSTGN 122
           GPKGDNLYHW ATI GP GTPYEGGIFFLDIIFPSDYPF PPK+VFKTRIYHCNVD+ G+
Sbjct: 66  GPKGDNLYHWIATIIGPSGTPYEGGIFFLDIIFPSDYPFKPPKLVFKTRIYHCNVDTAGD 125

Query: 123 VSLDILKDNWSPALTIAKVLIAVKSIFANPNP 154
           +S++IL+D+WSPALTI KVL A++SIF  P P
Sbjct: 126 LSVNILRDSWSPALTITKVLQAIRSIFLKPEP 150

[Close]

BLAST of HG10001069 vs. TAIR 10
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 187.2 bits (474), Expect = 1.2e-47
Identity = 77/145 (53.10%), Postives = 108/145 (74.48%), Query Frame = 0


Query: 40  SGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYP 99
           + KRI +E+ +   DPPS+CSAGP  ++++HW ATI GPP +PY GG+F + I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 100 FNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAP 159
           F PPKV FKT++YH N++S G++ LDILK+ WSPALTI+KVL+++ S+  +PNP DP  P
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLADRGKHDEIAAEWTLRFA 185
            +AH+Y  DR K++  A  WT ++A
Sbjct: 122 EIAHMYKTDRSKYESTARSWTQKYA 146

[Close]

BLAST of HG10001069 vs. TAIR 10
Match: AT3G08690.2 (ubiquitin-conjugating enzyme 11 )

HSP 1 Score: 187.2 bits (474), Expect = 1.2e-47
Identity = 77/145 (53.10%), Postives = 108/145 (74.48%), Query Frame = 0


Query: 40  SGKRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYP 99
           + KRI +E+ +   DPPS+CSAGP  ++++HW ATI GPP +PY GG+F + I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 100 FNPPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAP 159
           F PPKV FKT++YH N++S G++ LDILK+ WSPALTI+KVL+++ S+  +PNP DP  P
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 160 GVAHLYLADRGKHDEIAAEWTLRFA 185
            +AH+Y  DR K++  A  WT ++A
Sbjct: 122 EIAHMYKTDRSKYESTARSWTQKYA 146

[Close]

BLAST of HG10001069 vs. TAIR 10
Match: AT2G16740.1 (ubiquitin-conjugating enzyme 29 )

HSP 1 Score: 186.0 bits (471), Expect = 2.7e-47
Identity = 76/143 (53.15%), Postives = 108/143 (75.52%), Query Frame = 0


Query: 42  KRIQREMTEFNLDPPSDCSAGPKGDNLYHWFATIFGPPGTPYEGGIFFLDIIFPSDYPFN 101
           +RI +E+ E   DPP  CSAGP G++++HW ATI GP  +PY GG+F ++I FP DYPF 
Sbjct: 4   RRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDYPFK 63

Query: 102 PPKVVFKTRIYHCNVDSTGNVSLDILKDNWSPALTIAKVLIAVKSIFANPNPYDPNAPGV 161
           PPKVVF+T+++H N++S GN+ LDILKD WSPALTI+KVL+++ S+  +PNP DP  P +
Sbjct: 64  PPKVVFRTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 123

Query: 162 AHLYLADRGKHDEIAAEWTLRFA 185
           AH+Y  D+ K++ +A  WT ++A
Sbjct: 124 AHIYKTDKTKYEAMARSWTQKYA 146

The following BLAST results are available for this feature:

BLAST of HG10001069 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902201.1	5.1e-101	96.76	constitutive photomorphogenesis protein 10 isoform X2 [Benincasa hispida]	[more]
XP_038902200.1	1.9e-100	96.74	constitutive photomorphogenesis protein 10 isoform X1 [Benincasa hispida]	[more]
XP_022943567.1	7.4e-100	95.68	constitutive photomorphogenesis protein 10 [Cucurbita moschata] >XP_022986179.1 ...	[more]
KAG6570333.1	7.4e-100	95.68	Constitutive photomorphogenesis protein 10, partial [Cucurbita argyrosperma subs...	[more]
XP_004142171.1	2.8e-99	96.22	constitutive photomorphogenesis protein 10 [Cucumis sativus] >KGN54065.1 hypothe...	[more]

BLAST of HG10001069 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJD7	1.8e-69	68.48	Constitutive photomorphogenesis protein 10 OS=Arabidopsis thaliana OX=3702 GN=CO...	[more]
Q2T9X7	1.9e-53	55.49	Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus OX=9913 GN=UBE2E3 PE=2 SV=1	[more]
Q969T4	1.9e-53	55.49	Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens OX=9606 GN=UBE2E3 PE=1 SV=1	[more]
P52483	1.9e-53	55.49	Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus OX=10090 GN=Ube2e3 PE=1 SV=2	[more]
Q96LR5	3.5e-52	58.55	Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens OX=9606 GN=UBE2E2 PE=1 SV=1	[more]

BLAST of HG10001069 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JFC7	3.6e-100	95.68	constitutive photomorphogenesis protein 10 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1FTD1	3.6e-100	95.68	constitutive photomorphogenesis protein 10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KWU2	1.4e-99	96.22	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280420 PE...	[more]
A0A5D3DDP3	2.8e-97	94.59	Constitutive photomorphogenesis protein 10 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BMW7	2.8e-97	94.59	constitutive photomorphogenesis protein 10 OS=Cucumis melo OX=3656 GN=LOC1034915...	[more]

BLAST of HG10001069 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13550.1	1.3e-70	68.48	Ubiquitin-conjugating enzyme family protein	[more]
AT3G13550.2	3.1e-56	68.42	Ubiquitin-conjugating enzyme family protein	[more]
AT3G08690.1	1.2e-47	53.10	ubiquitin-conjugating enzyme 11	[more]
AT3G08690.2	1.2e-47	53.10	ubiquitin-conjugating enzyme 11	[more]
AT2G16740.1	2.7e-47	53.15	ubiquitin-conjugating enzyme 29	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 42..185 e-value: 1.6E-60 score: 217.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..40
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..40
None	No IPR available	PANTHER	PTHR24068:SF62	UBIQUITIN-CONJUGATING ENZYME E2 E2	coord: 27..184
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 27..184
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 30..185 e-value: 8.4E-59 score: 199.7
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 29..184
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 43..178 e-value: 1.8E-42 score: 144.3
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 39..185 score: 50.829475
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 42..180 e-value: 6.4277E-61 score: 183.943

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001069.1	HG10001069.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0010099	regulation of photomorphogenesis
cellular_component	GO:0005634	nucleus
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity