HG10001020 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001020
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr09: 13305380 .. 13306225 (+)
RNA-Seq ExpressionHG10001020
SyntenyHG10001020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGGAAATCCCCGTGGGCGCCGAAGATTCAGACATCTAGAGTTTGATGAAGATGATGATGATAATGAGCATGAGGATGGGGAGGGGAATCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGCTGAATCGGAGAATAGTTACGAAGGAGAGAGGGGCAGAGAAAGAGAGCGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCCAACAGATATGGCACTCCACCTGCACAGAAGGAGGCAGTTGATGCGCTGCTCACCGTGAGGGTTGAACTTGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGGTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGCAGGTGGTGGTGGGTCTGCTAGATTCATGGGCGACCTTAATAATGCTAATGGTGGCAGTGGCAGCGAGGAGATTGATGGAAGAACTGCATCTGGGTCTGGTAGAAGGTTTTCGTTTCCATGGCCCTTCAATGGCTTGTTTTCTTCTCAAGGAAACTCATCAGGTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

mRNA sequence

ATGATGGGAAATCCCCGTGGGCGCCGAAGATTCAGACATCTAGAGTTTGATGAAGATGATGATGATAATGAGCATGAGGATGGGGAGGGGAATCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGCTGAATCGGAGAATAGTTACGAAGGAGAGAGGGGCAGAGAAAGAGAGCGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCCAACAGATATGGCACTCCACCTGCACAGAAGGAGGCAGTTGATGCGCTGCTCACCGTGAGGGTTGAACTTGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGGTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGCAGGTGGTGGTGGGTCTGCTAGATTCATGGGCGACCTTAATAATGCTAATGGTGGCAGTGGCAGCGAGGAGATTGATGGAAGAACTGCATCTGGGTCTGGTAGAAGGTTTTCGTTTCCATGGCCCTTCAATGGCTTGTTTTCTTCTCAAGGAAACTCATCAGGTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Coding sequence (CDS)

ATGATGGGAAATCCCCGTGGGCGCCGAAGATTCAGACATCTAGAGTTTGATGAAGATGATGATGATAATGAGCATGAGGATGGGGAGGGGAATCGTGTGAGTAGTGAGCCGACTGAGCTTGATTCAGTCATTAGGAGGAGGAGAAGTTCAGCTACCATTCTTCAGTTGCTCCAAGGTATCAGAGCTGGAATGGCAACTGCTGAATCGGAGAATAGTTACGAAGGAGAGAGGGGCAGAGAAAGAGAGCGGGTTATTCTTATAAATCCTTTCAATCAGACCATTGTAGTTCAAGGAGGTGAGAATCAAAATCAAAACCCCATTGGTTCGCTGGGTGATTATTTCGTAGGACCTGGTTTGGATTTGTTATTGCAACATCTAGCCGAAAATGATCCCAACAGATATGGCACTCCACCTGCACAGAAGGAGGCAGTTGATGCGCTGCTCACCGTGAGGGTTGAACTTGAGGATAGCCGTTTGCAATGTTCAGTGTGTTTGGATGAGTTTGAGGTGGATGAAGAAGCCAAGGAGATGCCATGTAAGCATAAATTTCATACAGGGTGTATTCTGCCATGGCTGGAGCTGCATAGTTCTTGCCCAGTTTGTAGGCATCAATTGCCTGGGGATGAATCCAAGCGTGATGCAGGTGGTGGTGGGTCTGCTAGATTCATGGGCGACCTTAATAATGCTAATGGTGGCAGTGGCAGCGAGGAGATTGATGGAAGAACTGCATCTGGGTCTGGTAGAAGGTTTTCGTTTCCATGGCCCTTCAATGGCTTGTTTTCTTCTCAAGGAAACTCATCAGGTGGAAGTGCAAGTGATTCTCAAAGGGATCACACTTCCATTTGA

Protein sequence

MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGIRAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI
Homology
BLAST of HG10001020 vs. NCBI nr
Match: XP_038901761.1 (E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida])

HSP 1 Score: 520.8 bits (1340), Expect = 7.4e-144
Identity = 262/281 (93.24%), Postives = 266/281 (94.66%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPT+LDSVIRRRRSSATILQLLQGI
Sbjct: 77  MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTDLDSVIRRRRSSATILQLLQGI 136

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQNPIGSLGDYFVGPGLD 120
           RAGMATAESENSYEGER RERERVILINPFNQTIVVQGGENQNQNPIGSLGDYFVGPGLD
Sbjct: 137 RAGMATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNPIGSLGDYFVGPGLD 196

Query: 121 LLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEFEVDEEAKEMPCK 180
           LLLQHLAENDPNRYGTPPAQKEAVDAL TVRVE  DS LQC+VCLDEFEVDEEAKEMPCK
Sbjct: 197 LLLQHLAENDPNRYGTPPAQKEAVDALPTVRVEPVDSCLQCAVCLDEFEVDEEAKEMPCK 256

Query: 181 HKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSEEIDG 240
           HKFHTGCILPWLELHSSCPVCRHQLPGDESKRDA  GGS RFMGDLNNANG    E IDG
Sbjct: 257 HKFHTGCILPWLELHSSCPVCRHQLPGDESKRDA-DGGSTRFMGDLNNANGSGSGEIIDG 316

Query: 241 RTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           R ASG+GRRFSFPWPFNGLFS+QGNSSGGSASDSQRDH SI
Sbjct: 317 RNASGTGRRFSFPWPFNGLFSAQGNSSGGSASDSQRDHASI 356

BLAST of HG10001020 vs. NCBI nr
Match: XP_008449904.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0040122.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK21744.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 502.7 bits (1293), Expect = 2.1e-138
Identity = 258/287 (89.90%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFD+DDDDNEHEDGEGN VSSE TELDSVIRRRRSSATILQLLQGI
Sbjct: 80  MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELDSVIRRRRSSATILQLLQGI 139

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQ--NPIGSLGDYFVGPG 120
           RAG+ATAESENSYEGER RERERVILINPFNQTIVVQGGENQNQ  N IGSLGDYFVGPG
Sbjct: 140 RAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPG 199

Query: 121 LDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVEL-EDSRLQCSVCLDEFEVDEEAKEM 180
           LDLLLQH+AENDPNRYGTPPAQKEAVDAL TVRVEL EDSRLQCSVCLDEFEVDEEAKEM
Sbjct: 200 LDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEM 259

Query: 181 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANG---GSG 240
           PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD  GG +ARFM DLNN NG   G+G
Sbjct: 260 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGNGNG 319

Query: 241 SEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           S EI+ R +S SGRRFSFPWPFNGLF+SQGNSS GSASDSQRDHTSI
Sbjct: 320 SGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI 366

BLAST of HG10001020 vs. NCBI nr
Match: XP_031739817.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus])

HSP 1 Score: 496.9 bits (1278), Expect = 1.1e-136
Identity = 257/284 (90.49%), Postives = 265/284 (93.31%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFD+DDDDNEHEDGEGNRVSSE TELDSVIRRRRSSATILQLLQGI
Sbjct: 80  MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRRRRSSATILQLLQGI 139

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGE--NQNQNPIGSLGDYFVGPG 120
           RAG+ATAESENSYE ER RERERVILINPFNQTIVVQGGE  NQNQN IGSLGDYFVGPG
Sbjct: 140 RAGIATAESENSYE-ERSRERERVILINPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPG 199

Query: 121 LDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVEL-EDSRLQCSVCLDEFEVDEEAKEM 180
           LDLLLQH+AENDPNRYGTPPAQKEAVDAL TVRVEL EDS LQCSVCLDEFEVDEEAKEM
Sbjct: 200 LDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEM 259

Query: 181 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSEE 240
           PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD  GG +ARFM DLNN N G+GS E
Sbjct: 260 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGN-GNGSGE 319

Query: 241 IDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           I+GR +S SGRRFSFPWPFNGLF+SQGNSS GSASDSQRDHTSI
Sbjct: 320 IEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI 361

BLAST of HG10001020 vs. NCBI nr
Match: KAG6570309.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.9 bits (1239), Expect = 3.8e-132
Identity = 254/293 (86.69%), Postives = 261/293 (89.08%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDN---EHEDGEGNRVSSEPTELDSVI-RRRRSSATILQL 60
           MMGNPRGRRRFRHLEFDEDDDDN   EH DGEGNRVSSEPTELDSVI RRRRSSATILQL
Sbjct: 63  MMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTELDSVIRRRRRSSATILQL 122

Query: 61  LQGIRAGMATAESENSYEGERG--------RERERVILINPFNQTIVVQGGENQNQNPIG 120
           LQGIRAGMAT ESENSYEGER         RERERVILINPFNQTIVVQGGE+QN N IG
Sbjct: 123 LQGIRAGMATGESENSYEGERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIG 182

Query: 121 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEF 180
           SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL TVRVE  D+RLQCSVCLDEF
Sbjct: 183 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEF 242

Query: 181 EVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNN 240
           E+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQLP DESKRDA  GGSARFMG    
Sbjct: 243 EMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDA-DGGSARFMG---- 302

Query: 241 ANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           +  GSGS EIDGR ASG+GRRFSFPWPFNGLFSSQGNSSGGS S+SQRDHTSI
Sbjct: 303 SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQRDHTSI 350

BLAST of HG10001020 vs. NCBI nr
Match: KAG7010206.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.9 bits (1239), Expect = 3.8e-132
Identity = 254/293 (86.69%), Postives = 261/293 (89.08%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDN---EHEDGEGNRVSSEPTELDSVI-RRRRSSATILQL 60
           MMGNPRGRRRFRHLEFDEDDDDN   EH DGEGNRVSSEPTELDSVI RRRRSSATILQL
Sbjct: 79  MMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTELDSVIRRRRRSSATILQL 138

Query: 61  LQGIRAGMATAESENSYEGERG--------RERERVILINPFNQTIVVQGGENQNQNPIG 120
           LQGIRAGMAT ESENSYEGER         RERERVILINPFNQTIVVQGGE+QN N IG
Sbjct: 139 LQGIRAGMATGESENSYEGERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIG 198

Query: 121 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEF 180
           SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL TVRVE  D+RLQCSVCLDEF
Sbjct: 199 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEF 258

Query: 181 EVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNN 240
           E+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQLP DESKRDA  GGSARFMG    
Sbjct: 259 EMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDA-DGGSARFMG---- 318

Query: 241 ANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           +  GSGS EIDGR ASG+GRRFSFPWPFNGLFSSQGNSSGGS S+SQRDHTSI
Sbjct: 319 SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSESESQRDHTSI 366

BLAST of HG10001020 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-41
Identity = 114/286 (39.86%), Postives = 163/286 (56.99%), Query Frame = 0

Query: 7   GRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGIRAGMAT 66
           G  ++  +E   +  D++ ED +  R      E +  IRR R ++T+ ++L  I   +A 
Sbjct: 55  GFSQWNPIEHPGETMDSDDEDNDLGR------EFEGFIRRHRRASTLRRVLDSIHDDLAD 114

Query: 67  AESENSYEGERGRERERVILINPFNQTIVVQGG----------ENQNQNPIGSLGDYFVG 126
                       +ER+  ILIN FNQ + +QG           +  + N  G L +Y +G
Sbjct: 115 -----------DQERDSSILINAFNQALALQGSVLDPDEGQGDQGGSTNDDGLLEEYVLG 174

Query: 127 PGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEFEVDEEAKE 186
            GL LLLQHLAE+DP+R GTPPA+KEAV+AL TV++E     + CSVCLD+ EV  +AK+
Sbjct: 175 AGLSLLLQHLAESDPSRNGTPPAKKEAVEALPTVKIE---EVVSCSVCLDDLEVGSQAKQ 234

Query: 187 MPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSE 246
           MPC+HKFH+ CILPWLELHSSCPVCR +LP +E+K D     +   + D +      G  
Sbjct: 235 MPCEHKFHSSCILPWLELHSSCPVCRFELPSEETK-DLNEPSNIGRVEDSHEEVRADG-- 294

Query: 247 EIDGRTASGSGRRFSF-PWPFNGLFSS-QGNSSGGSASDSQRDHTS 281
              G  +  S R ++  PW  N LFS+ +  ++GG ++D Q  HTS
Sbjct: 295 --PGNVSESSNRPWAIVPW-LNELFSTREAQNAGGVSTDQQSPHTS 314

BLAST of HG10001020 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 9.7e-38
Identity = 84/172 (48.84%), Postives = 107/172 (62.21%), Query Frame = 0

Query: 109 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRV---ELEDSRLQCSVCL 168
           ++GDYF+GPGL+ L+Q LAENDPNRYGTPPA K A++AL  V +    L     QC+VC+
Sbjct: 169 NIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCM 228

Query: 169 DEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGD 228
           D+FE   EAK+MPCKH +H  C+LPWLELH+SCPVCRH+LP D+   +    G+    G 
Sbjct: 229 DDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGG 288

Query: 229 LNNANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRD 278
            +  N G  S+  D RT   S  R S PWPF       G + G +A   Q D
Sbjct: 289 NDGDNSGQRSDG-DNRTVERS-FRISLPWPFQA--RGPGPAPGDNAETRQED 336

BLAST of HG10001020 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 8.2e-37
Identity = 106/277 (38.27%), Postives = 137/277 (49.46%), Query Frame = 0

Query: 24  EHEDGEGNR-VSSEPTELDSVIRRRRSSATILQLLQGIRAGMATAESENSYEGERGRERE 83
           E+ED   N+ ++  P   DS        +T+L L+ G  A   +     S  G   + + 
Sbjct: 52  EYEDPNPNQSLNFNPNSSDSFFPMADPFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQA 111

Query: 84  RVILINPFN-------------QTIVVQGG------ENQNQNP----IGSLGDYFVGPGL 143
           R    NP +             QT+   G       EN   +P     G+ GDYF GPGL
Sbjct: 112 RSTQQNPQSDAFDPFTFLQNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGL 171

Query: 144 DLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVE---LEDSRLQCSVCLDEFEVDEEAKE 203
           + L+Q LAENDPNRYGTPPA K A+DAL TV+V    L+    QC+VC+DEFE   + K+
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQ 231

Query: 204 MPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSE 263
           MPCKH FH  C+LPWLELH+SCPVCR +LP D+   +             N + G  GS 
Sbjct: 232 MPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYE-------------NRSQGSQGSG 291

Query: 264 EIDGRTASGSGRRFS--FPWPFNGLFSSQGNSSGGSA 272
           +  G        RFS   PWPF       G+ SG  A
Sbjct: 292 DGQGSVEGQQTPRFSIQLPWPFR---RQDGSGSGSGA 312

BLAST of HG10001020 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.2e-25
Identity = 72/177 (40.68%), Postives = 96/177 (54.24%), Query Frame = 0

Query: 108 GSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRV---ELEDSRLQCSVC 167
           G+ GDYF GPGL+ L + L+     R G PPA + A+DAL T+++    L  S   C VC
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGTTRR-GPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 168 LDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMG 227
            DEFE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP       +G   S     
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS-----ASGPSSSQNRTT 254

Query: 228 DLNNANGGSGSEEIDGRTASGSGRR--FSFPWPF--NGLFSSQGNSSGGSASDSQRD 278
              N    S S   + R  +G+ RR  FS  WPF  +G  SS   + GG+ +    D
Sbjct: 255 PTRNYRSSSSSSSSNSR-ENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNSDTSD 304

BLAST of HG10001020 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-24
Identity = 66/170 (38.82%), Postives = 93/170 (54.71%), Query Frame = 0

Query: 111 GDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRV---ELEDSRLQCSVCLDE 170
           GDYF GPGL+ L++ L+    +R G PPA K ++DAL T+++    L+ S   C VC DE
Sbjct: 134 GDYFFGPGLEELIEQLSSGTHHR-GPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDE 193

Query: 171 FEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLN 230
           FE+  EAK+MPC H +H+ CI+PWL  H+SCPVCR +LP   S                +
Sbjct: 194 FELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS----------------S 253

Query: 231 NANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRD 278
           ++   S +   +GR  S     FS  WPF         SS  S++ ++RD
Sbjct: 254 SSTQSSQNRSTNGRENSRRRNIFSNLWPF--------RSSSSSSTQNRRD 278

BLAST of HG10001020 vs. ExPASy TrEMBL
Match: A0A0A0KWR3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.6e-139
Identity = 258/284 (90.85%), Postives = 266/284 (93.66%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFD+DDDDNEHEDGEGNRVSSE TELDSVIRRRRSSATILQLLQGI
Sbjct: 80  MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNRVSSETTELDSVIRRRRSSATILQLLQGI 139

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGE--NQNQNPIGSLGDYFVGPG 120
           RAG+ATAESENSYEGER RERERVILINPFNQTIVVQGGE  NQNQN IGSLGDYFVGPG
Sbjct: 140 RAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNQNSIGSLGDYFVGPG 199

Query: 121 LDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVEL-EDSRLQCSVCLDEFEVDEEAKEM 180
           LDLLLQH+AENDPNRYGTPPAQKEAVDAL TVRVEL EDS LQCSVCLDEFEVDEEAKEM
Sbjct: 200 LDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSCLQCSVCLDEFEVDEEAKEM 259

Query: 181 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANGGSGSEE 240
           PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD  GG +ARFM DLNN N G+GS E
Sbjct: 260 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGN-GNGSGE 319

Query: 241 IDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           I+GR +S SGRRFSFPWPFNGLF+SQGNSS GSASDSQRDHTSI
Sbjct: 320 IEGRNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI 362

BLAST of HG10001020 vs. ExPASy TrEMBL
Match: A0A1S3BNR7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.0e-138
Identity = 258/287 (89.90%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFD+DDDDNEHEDGEGN VSSE TELDSVIRRRRSSATILQLLQGI
Sbjct: 80  MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELDSVIRRRRSSATILQLLQGI 139

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQ--NPIGSLGDYFVGPG 120
           RAG+ATAESENSYEGER RERERVILINPFNQTIVVQGGENQNQ  N IGSLGDYFVGPG
Sbjct: 140 RAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPG 199

Query: 121 LDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVEL-EDSRLQCSVCLDEFEVDEEAKEM 180
           LDLLLQH+AENDPNRYGTPPAQKEAVDAL TVRVEL EDSRLQCSVCLDEFEVDEEAKEM
Sbjct: 200 LDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEM 259

Query: 181 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANG---GSG 240
           PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD  GG +ARFM DLNN NG   G+G
Sbjct: 260 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGNGNG 319

Query: 241 SEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           S EI+ R +S SGRRFSFPWPFNGLF+SQGNSS GSASDSQRDHTSI
Sbjct: 320 SGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI 366

BLAST of HG10001020 vs. ExPASy TrEMBL
Match: A0A5D3DDU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001160 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.0e-138
Identity = 258/287 (89.90%), Postives = 266/287 (92.68%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQLLQGI 60
           MMGNPRGRRRFRHLEFD+DDDDNEHEDGEGN VSSE TELDSVIRRRRSSATILQLLQGI
Sbjct: 80  MMGNPRGRRRFRHLEFDDDDDDNEHEDGEGNHVSSETTELDSVIRRRRSSATILQLLQGI 139

Query: 61  RAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQ--NPIGSLGDYFVGPG 120
           RAG+ATAESENSYEGER RERERVILINPFNQTIVVQGGENQNQ  N IGSLGDYFVGPG
Sbjct: 140 RAGIATAESENSYEGERSRERERVILINPFNQTIVVQGGENQNQNHNSIGSLGDYFVGPG 199

Query: 121 LDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVEL-EDSRLQCSVCLDEFEVDEEAKEM 180
           LDLLLQH+AENDPNRYGTPPAQKEAVDAL TVRVEL EDSRLQCSVCLDEFEVDEEAKEM
Sbjct: 200 LDLLLQHIAENDPNRYGTPPAQKEAVDALPTVRVELEEDSRLQCSVCLDEFEVDEEAKEM 259

Query: 181 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNNANG---GSG 240
           PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRD  GG +ARFM DLNN NG   G+G
Sbjct: 260 PCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDVDGGSAARFMSDLNNGNGNGNGNG 319

Query: 241 SEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           S EI+ R +S SGRRFSFPWPFNGLF+SQGNSS GSASDSQRDHTSI
Sbjct: 320 SGEIEERNSSESGRRFSFPWPFNGLFTSQGNSSAGSASDSQRDHTSI 366

BLAST of HG10001020 vs. ExPASy TrEMBL
Match: A0A6J1FWF9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.4e-132
Identity = 253/293 (86.35%), Postives = 262/293 (89.42%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDN---EHEDGEGNRVSSEPTELDSVI-RRRRSSATILQL 60
           MMGNPRGRRRFRHLEFDEDDDDN   EH DGEGNRVSSEPTELDSVI RRRRSSATILQL
Sbjct: 80  MMGNPRGRRRFRHLEFDEDDDDNNDQEHGDGEGNRVSSEPTELDSVIRRRRRSSATILQL 139

Query: 61  LQGIRAGMATAESENSYEGERG--------RERERVILINPFNQTIVVQGGENQNQNPIG 120
           LQGIRAGMAT ESENSYEGER         RERERVILINPFNQTIVVQGGE+QN N IG
Sbjct: 140 LQGIRAGMATGESENSYEGERSRERERERERERERVILINPFNQTIVVQGGESQNHNAIG 199

Query: 121 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEF 180
           SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL TVRVE  D+RLQCSVCLDEF
Sbjct: 200 SLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLDEF 259

Query: 181 EVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDLNN 240
           E+DEEAKEMPC+HKFH+GCILPWLELHSSCPVCRHQLP DESKRDA  GGSARFMG  + 
Sbjct: 260 EMDEEAKEMPCRHKFHSGCILPWLELHSSCPVCRHQLPADESKRDA-DGGSARFMG--SG 319

Query: 241 ANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           +  GSGS EIDGR ASG+GRRFSFPWPFNGLFSSQGNSSGGS S+SQRDHTSI
Sbjct: 320 SGSGSGSGEIDGRNASGTGRRFSFPWPFNGLFSSQGNSSGGSGSESQRDHTSI 369

BLAST of HG10001020 vs. ExPASy TrEMBL
Match: A0A6J1JDY5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.0e-132
Identity = 253/298 (84.90%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDN---EHEDGEGNRVSSEPTELDSVI-RRRRSSATILQL 60
           MMGNPRGRRRFRHLEFDEDDD+N   EH DGEGNR SSEP ELDSVI RRRRSSATILQL
Sbjct: 79  MMGNPRGRRRFRHLEFDEDDDNNNEQEHGDGEGNRASSEPAELDSVIRRRRRSSATILQL 138

Query: 61  LQGIRAGMATAESENSYEGERG----------RERERVILINPFNQTIVVQGGENQNQNP 120
           LQGIRAGMAT ESENSYEGER           RERERVILINPFNQTIVVQGGE+QN N 
Sbjct: 139 LQGIRAGMATGESENSYEGERSRERERERERERERERVILINPFNQTIVVQGGESQNHNA 198

Query: 121 IGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLD 180
           IGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDAL TVRVE  D+RLQCSVCLD
Sbjct: 199 IGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALPTVRVECGDNRLQCSVCLD 258

Query: 181 EFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAGGGGSARFMGDL 240
           EFE+DEEAKEMPCKHKFH+GCILPWLELHSSCPVCRHQLP DESKRD   GGSARFMG+L
Sbjct: 259 EFEMDEEAKEMPCKHKFHSGCILPWLELHSSCPVCRHQLPADESKRDT-DGGSARFMGEL 318

Query: 241 NNAN---GGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQRDHTSI 282
           +N N    GSGS EIDGRTASG GRRFSFPWPFNGLFSSQGNSSGGS S+SQRDHT I
Sbjct: 319 SNGNDSGSGSGSGEIDGRTASGIGRRFSFPWPFNGLFSSQGNSSGGSESESQRDHTPI 375

BLAST of HG10001020 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.0e-74
Identity = 162/309 (52.43%), Postives = 200/309 (64.72%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDDN-----EHEDGEGNRVSSE------------------- 60
           MM +PR RRRFR  EF E++DDN      + DG GN  ++                    
Sbjct: 77  MMSSPRRRRRFRRSEFGEENDDNGDDLTTNADGNGNDSNNSNNNVYRHHRARRHGGEIDL 136

Query: 61  PTELDSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERGRERERVILINPFNQTIV 120
             E +S++ RRRRSS  ILQLLQGIRAG+A+    +    +  RER+RVI+INP+NQ++V
Sbjct: 137 DREFESILRRRRRSSGNILQLLQGIRAGIASEYESSDNNWDNSRERDRVIMINPYNQSLV 196

Query: 121 VQGGENQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELE 180
           V   +NQN   + SLGDYF+GPGLDLLLQHLAENDPNR GTPPA+KEAV+AL TV++   
Sbjct: 197 VPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQGTPPARKEAVEALPTVKIM-- 256

Query: 181 DSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPG-----DES 240
              LQCSVCLD+FE   EAKEMPCKHKFH  CI+PWLELHSSCPVCR +LP      DE+
Sbjct: 257 -EPLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPSSADDDDET 316

Query: 241 KRDAGGGGSARFMGDLNNA----NGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNS 276
           K D+      R + + +N     N G+     +    SG+GRRFSFPWPF+GLFSS  +S
Sbjct: 317 KTDSERVLRTRNVRETSNGNVVENVGNADRGREDEVRSGNGRRFSFPWPFSGLFSS-SSS 376

BLAST of HG10001020 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 241.9 bits (616), Expect = 6.2e-64
Identity = 145/293 (49.49%), Postives = 186/293 (63.48%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDD-----DNEHEDGEGNRVSSEPTELDSVIRRRRSSATILQ 60
           +M +P  RRR + +E  ED+      +N  +DGE N +  +  E+  + RRRR SA +LQ
Sbjct: 67  LMNDPVRRRRNQSVESVEDNQNEVQTENNEDDGE-NDLDWQLQEI--LRRRRRHSAAVLQ 126

Query: 61  LLQGIRAGMATAESENSYEGERGRERERVILINPFNQTIVVQGGENQNQNPIGSLGDYFV 120
           LLQGIRAG+ + ESE++  G  G    RVILIN  NQTI VQ   + +  P GSLGDYF+
Sbjct: 127 LLQGIRAGL-SVESEST--GNGGNNPGRVILINTSNQTITVQNSADMDSVPAGSLGDYFI 186

Query: 121 GPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEFEVDEEAK 180
           GPG ++LLQ LAENDPNRYGTPPA+KEAV+AL TV++E     LQCSVCLD+FE+  EAK
Sbjct: 187 GPGFEMLLQRLAENDPNRYGTPPAKKEAVEALATVKIE---ETLQCSVCLDDFEIGTEAK 246

Query: 181 EMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDA------GGGGSARFMGDLNNA 240
            MPC HKFH+ C+LPWLELHSSCPVCR+QLP DE+K D+        G S+      + A
Sbjct: 247 LMPCTHKFHSDCLLPWLELHSSCPVCRYQLPADEAKTDSVTTTSDNNGSSSASATTSHGA 306

Query: 241 NGGSGS----------EEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSAS 273
               G+          EE +    +  G  FS PWPF+ LFSS  +S+  + S
Sbjct: 307 ENSDGNRRQEEEEEEEEEEEEENENNDGSGFSIPWPFSTLFSSSQDSNAPTDS 350

BLAST of HG10001020 vs. TAIR 10
Match: AT4G26400.2 (RING/U-box superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 8.7e-58
Identity = 142/305 (46.56%), Postives = 184/305 (60.33%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDD-NEHEDGEGN-------------------RVSSEPTEL 60
           MM NPR RRRFR  EF  D+D+ N  +D +GN                   R      E 
Sbjct: 73  MMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQQGREIDLDREF 132

Query: 61  DSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERGRERERVILINPFNQTIVVQG- 120
           +S++ RRRRSSATILQLLQGIRAG+A +E E+S         +R +L    NQ+ VVQG 
Sbjct: 133 ESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRDLL----NQSAVVQGS 192

Query: 121 ---GENQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELE 180
               +N+N   + ++GDYFVG  LD LL+HLA+ND  R+G+ PA+KE VD L TV++   
Sbjct: 193 TSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKI--- 252

Query: 181 DSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAG 240
              LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCPVCR++LP D+  +   
Sbjct: 253 SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETKVNP 312

Query: 241 GGGSARFMGDLNNANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQ 281
                R +      N  + + E D R ++ S RRFSFPWPF+GLFSS  +SS  ++  S 
Sbjct: 313 VRPRTRTL----EINVSNENVEDDARNSNVSERRFSFPWPFSGLFSSSSSSSASASGSSH 356

BLAST of HG10001020 vs. TAIR 10
Match: AT4G26400.1 (RING/U-box superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 8.7e-58
Identity = 142/305 (46.56%), Postives = 184/305 (60.33%), Query Frame = 0

Query: 1   MMGNPRGRRRFRHLEFDEDDDD-NEHEDGEGN-------------------RVSSEPTEL 60
           MM NPR RRRFR  EF  D+D+ N  +D +GN                   R      E 
Sbjct: 73  MMSNPRRRRRFRRTEFGVDNDEVNVSDDVDGNDSVDIDRHHHRRHRHRQQGREIDLDREF 132

Query: 61  DSVI-RRRRSSATILQLLQGIRAGMATAESENSYEGERGRERERVILINPFNQTIVVQG- 120
           +S++ RRRRSSATILQLLQGIRAG+A +E E+S         +R +L    NQ+ VVQG 
Sbjct: 133 ESILRRRRRSSATILQLLQGIRAGIA-SEYESS---------DRDLL----NQSAVVQGS 192

Query: 121 ---GENQNQNPIGSLGDYFVGPGLDLLLQHLAENDPNRYGTPPAQKEAVDALLTVRVELE 180
               +N+N   + ++GDYFVG  LD LL+HLA+ND  R+G+ PA+KE VD L TV++   
Sbjct: 193 TSLNQNRNNTSLSAIGDYFVGSSLDHLLEHLADNDSIRHGSLPARKEVVDNLPTVKI--- 252

Query: 181 DSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPWLELHSSCPVCRHQLPGDESKRDAG 240
              LQCS+CLD+F+   EAKEMPCKHKFH  CI+PWLELHSSCPVCR++LP D+  +   
Sbjct: 253 SESLQCSICLDDFDKGSEAKEMPCKHKFHIRCIVPWLELHSSCPVCRYELPPDDETKVNP 312

Query: 241 GGGSARFMGDLNNANGGSGSEEIDGRTASGSGRRFSFPWPFNGLFSSQGNSSGGSASDSQ 281
                R +      N  + + E D R ++ S RRFSFPWPF+GLFSS  +SS  ++  S 
Sbjct: 313 VRPRTRTL----EINVSNENVEDDARNSNVSERRFSFPWPFSGLFSSSSSSSASASGSSH 356

BLAST of HG10001020 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.1e-51
Identity = 123/238 (51.68%), Postives = 154/238 (64.71%), Query Frame = 0

Query: 17  DEDDDDNEHEDGEGNRVSSEPT--ELDSVIR-RRRSSATILQLLQGIRAGMATAESENSY 76
           DED+D+++ +DG+ N         +L+ + R R R S  I+ LLQGIRAG+   ESEN+ 
Sbjct: 81  DEDEDEDDGDDGDQNNDGEIDITHQLEEIRRIRTRHSTAIVNLLQGIRAGL-LIESENNE 140

Query: 77  EGERGRERERVILINPFNQTI-VVQGGENQNQNPIGSLGDYFVGPGLDLLLQHLAEND-P 136
           +       E V+LIN FNQ I V Q   +    P GSLGDYF+GPG + LLQ LAEND  
Sbjct: 141 DNP--DNSELVVLINSFNQRIRVHQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLN 200

Query: 137 NRYGTPPAQKEAVDALLTVRVELEDSRLQCSVCLDEFEVDEEAKEMPCKHKFHTGCILPW 196
           NRYGTPPA KEAV+AL  V++  EDS LQCSVCLD+FE+  EAKEMPCKHKFH+ C+LPW
Sbjct: 201 NRYGTPPATKEAVEALAMVKI--EDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCLLPW 260

Query: 197 LELHSSCPVCRHQLP---GDESKRDAGGGGSARFMGDLNNANGGSGSEEIDGRTASGS 247
           LELHSSCPVCR+ LP    DE K DA    +     D++NA+  S     D  + + S
Sbjct: 261 LELHSSCPVCRYLLPTGDDDEPKTDAETSRNDDNNEDISNASMASNGSSPDSSSNNSS 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901761.17.4e-14493.24E3 ubiquitin-protein ligase SIRP1 [Benincasa hispida] >XP_038901762.1 E3 ubiquit... [more]
XP_008449904.12.1e-13889.90PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008449905.1... [more]
XP_031739817.11.1e-13690.49LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SIRP1 [Cucumis sativus][more]
KAG6570309.13.8e-13286.69E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7010206.13.8e-13286.69E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q6AVN21.5e-4139.86E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
P0CH309.7e-3848.84E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN78.2e-3738.27E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
O221973.2e-2540.68Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q94AK42.1e-2438.82E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0KWR34.6e-13990.85RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G267990 PE... [more]
A0A1S3BNR71.0e-13889.90RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491641 PE=4 ... [more]
A0A5D3DDU31.0e-13889.90RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1FWF92.4e-13286.35RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448781... [more]
A0A6J1JDY57.0e-13284.90RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111483664 P... [more]
Match NameE-valueIdentityDescription
AT5G56340.11.0e-7452.43RING/U-box superfamily protein [more]
AT1G55530.16.2e-6449.49RING/U-box superfamily protein [more]
AT4G26400.28.7e-5846.56RING/U-box superfamily protein [more]
AT4G26400.18.7e-5846.56RING/U-box superfamily protein [more]
AT3G13430.12.1e-5151.68RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 161..201
e-value: 7.0E-8
score: 42.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 160..202
e-value: 2.7E-13
score: 50.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 161..202
score: 12.386007
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 125..208
e-value: 8.0E-21
score: 75.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 226..240
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 208..246
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availablePANTHERPTHR15710:SF22RING/U-BOX SUPERFAMILY PROTEINcoord: 1..257
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 1..257
NoneNo IPR availableCDDcd16669RING-H2_RNF181coord: 160..205
e-value: 1.01577E-24
score: 91.6633
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 154..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001020.1HG10001020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity