HG10000995 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000995
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionClathrin light chain
LocationChr09: 12925485 .. 12927884 (+)
RNA-Seq ExpressionHG10000995
SyntenyHG10000995
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCTTCGACACTTTCATCAACGAAGAACACGAGCAACAGCATCACCATTCCCCTACGGCGCAACCCTTTGACGATGATGGCTACGTCGGATTTGACCCTCATCTCCCCTCCCAACGCTATGACTCTTCTACCCCTTTCTCTCCCTCCGACGATATTACCTCCGCCGACCAACACCCCCATGCTCCCCCTTCCTTTTTCGAAGACGACGTCATTTCCGACATCCACACCGCTGGAAACACCACTAATCCTAATTTCTCTGACGCTTACGATTTTGGAGTTTCCAATCCTAATCCCGACTATGTATCTCCCTTTGATTCTGTTCACGCCGATGGAGACCATGACAATGCCGCCCCCGCCCCCGCCCCCGCCCCCTTCGGTGGTGGTGGTGGTGGTGGTTTTGACGATGGTGGTATTTTTGTCTCTGATGGACCTGTTCTCCCGGACCCGGGCGAAATGCGGGAGGAAGGCCATGCTCGACGAGAATGGCGACGGTGCGTTTTTTTATTACTATTATTGTTATTTCTTCATGAGAGATTGGATTGGTTTTAGATCTGTGATTTTATCATTTTGGGAAGGATTGAAATGTTTCCTTCTGTGGCTGAATCTGAAATGAAATCGTTTATAAATTTTGAGGTCGTAAAATATAGATCTTTGTTACTAACTTTCATCCCGCATGAGTGCCAATCGTAGAGCTCTGTGTTTACGCAGCTGAAGCGTGGTCTTATGATTTGTACAGGGGTTTCTTAGTTTTTATAACAATTGCCATCTAGCTCGTGTTTGTAGTGGCTTCTCACTAATTGAATCCCTTACTGGTGATTTTTTTTGGAATCTCATTTGCTTCTGGAACTATGAAGAGTTAATCATTTGTTGCAATATATCTGACATCAAATATGTAGGTGTATTTGTTTATTGTAATTCATCTGTGTCCGATACTGTAATGTCCAAACAATAGAGTTCTACGCCTGGTACAAGACAGATATTGGCTGCAGATTTGTCCTAAGTCTAACACCACGTGTATGCATAAATGTCCGACATATTAGTAAGACCAACTATTTACAAGTTTATTACAGTATTTTATGTATTATAGCTATGGGGACAGGAAAAAGGGGTATGCTTAATTCTGTCTGCTTGAAGCCTGAGTCTTTTGGAAGTTTGATGATGTGGAAAATTCTTATCTCTATTATGTATTATGTGTGTGAGCTGGCTTCATGGGCCACAGTGGTTTCATCTTTCAAGTTCCTGGGAAGTAATGGTTACCCAGCTTCCATTTTTTCTTGGAATTTCCATTTTGATCCCTGGCTAATTGTAAAGATTAGGGCTGTGATCGTTTCAAAATTTTCTGTCTTGCTGCACAAGCTGGGTCAAGTGAGGTGGAAGGGGAATTGTTTGGGAACCATGTTTCTCTGCTCTCTCTTATTTTCAAGAGAAATCATTTTCTCATATTTTTTCATCTGTTCTTAGCTGTTTGCTAATAATCTGAAAACTTTATAGAGCAGTCAAAATGCCATAGATCTTGAGGAGAAAGAGAAGAAAGAAAAGGAGATGAGGAATCAAATAATTAATGAAGCTGAAGAGTATAAAGCATCCTTTTATGAGAAAAGGAGGGTCAATTGTGAAACAAATAAGGCTCACAATAGAGAAAGAGAGAAGGTGAAACCTCTGAAGCATTTTGTATATATTCATATTCTTGATCCTGCATAATGTCAATGTCTCCTTTCTATGTATTGTTATTTATTTTTCATTTCTTTGCAGCTTTACCATGCTAACCAGGAAAAATTTCACAAAGAAGCTGACAAACATTACTGGAAAGCAATTGCAGAGATCATCCCACGAGAGGTACCTAATATTGAGAAGAGAAGAGGAAAGAAAGATGCTGACAATAAGCCTTCAATAGTGGTTGTTCAGGGTCCAAAACCTGGAAAACCAACCGATCTGTCACGAATGAGGCAGATTCTGTTAAAGTTGAAACAGACTCCTCCACCTCATATGATGCCTCCTCCCCCCAAACCGGCCAAAGATGACAAGGATGGCAAAGATGGAAAGGATGGCAAAGATGGAAAAGATGGCAAGGATGGAAAGGATAGCAAGGAAAAGGATAGTAAGGATGGCAAGGATGGCAAAGATACAAAGGAAGGGAAGGAGGACAAGGAGGACAAAGGTGGAAAGGAAGCAAAGGAAGCAAAGGAAGGGACAGAAACCAAAGATGCTAAGGAAGAAGGGAAGGAAGTTGAGGACAAGAAAGCTTCTTCACCAGCTGCAGTTCACACACCTACATCTCCTGGAAAAGATATGGATACCAATGGTACTCCCGATCAACCCAAAATAGAGGTTGAGGCTCCTCCTGCGGCTGATACAGAACAAGCTGTTCTGTTGGTGTCACCTTCTGCAGAGTAA

mRNA sequence

ATGGCTTCCTTCGACACTTTCATCAACGAAGAACACGAGCAACAGCATCACCATTCCCCTACGGCGCAACCCTTTGACGATGATGGCTACGTCGGATTTGACCCTCATCTCCCCTCCCAACGCTATGACTCTTCTACCCCTTTCTCTCCCTCCGACGATATTACCTCCGCCGACCAACACCCCCATGCTCCCCCTTCCTTTTTCGAAGACGACGTCATTTCCGACATCCACACCGCTGGAAACACCACTAATCCTAATTTCTCTGACGCTTACGATTTTGGAGTTTCCAATCCTAATCCCGACTATGTATCTCCCTTTGATTCTGTTCACGCCGATGGAGACCATGACAATGCCGCCCCCGCCCCCGCCCCCGCCCCCTTCGGTGGTGGTGGTGGTGGTGGTTTTGACGATGGTGGTATTTTTGTCTCTGATGGACCTGTTCTCCCGGACCCGGGCGAAATGCGGGAGGAAGGCCATGCTCGACGAGAATGGCGACGTATTTTATGTATTATAGCTATGGGGACAGGAAAAAGGGGTATGCTTAATTCTGTCTGCTTGAAGCCTGAGTCTTTTGGAAGTTTGATGATGTGGAAAATTCTTATCTCTATTATGTATTATGTGTGTGAGCTGGCTTCATGGGCCACAGTGGTTTCATCTTTCAAGTTCCTGGGAAGTAATGGTTACCCAGCTTCCATTTTTTCTTGGAATTTCCATTTTGATCCCTGGCTAATTGTAAAGATTAGGGCTGTGATCGTTTCAAAATTTTCTGTCTTGCTGCACAAGCTGGGTCAAAGCAGTCAAAATGCCATAGATCTTGAGGAGAAAGAGAAGAAAGAAAAGGAGATGAGGAATCAAATAATTAATGAAGCTGAAGAGTATAAAGCATCCTTTTATGAGAAAAGGAGGGTCAATTGTGAAACAAATAAGGCTCACAATAGAGAAAGAGAGAAGCTTTACCATGCTAACCAGGAAAAATTTCACAAAGAAGCTGACAAACATTACTGGAAAGCAATTGCAGAGATCATCCCACGAGAGGTACCTAATATTGAGAAGAGAAGAGGAAAGAAAGATGCTGACAATAAGCCTTCAATAGTGGTTGTTCAGGGTCCAAAACCTGGAAAACCAACCGATCTGTCACGAATGAGGCAGATTCTGTTAAAGTTGAAACAGACTCCTCCACCTCATATGATGCCTCCTCCCCCCAAACCGGCCAAAGATGACAAGGATGGCAAAGATGGAAAGGATGGCAAAGATGGAAAAGATGGCAAGGATGGAAAGGATAGCAAGGAAAAGGATAGTAAGGATGGCAAGGATGGCAAAGATACAAAGGAAGGGAAGGAGGACAAGGAGGACAAAGGTGGAAAGGAAGCAAAGGAAGCAAAGGAAGGGACAGAAACCAAAGATGCTAAGGAAGAAGGGAAGGAAGTTGAGGACAAGAAAGCTTCTTCACCAGCTGCAGTTCACACACCTACATCTCCTGGAAAAGATATGGATACCAATGGTACTCCCGATCAACCCAAAATAGAGGTTGAGGCTCCTCCTGCGGCTGATACAGAACAAGCTGTTCTGTTGGTGTCACCTTCTGCAGAGTAA

Coding sequence (CDS)

ATGGCTTCCTTCGACACTTTCATCAACGAAGAACACGAGCAACAGCATCACCATTCCCCTACGGCGCAACCCTTTGACGATGATGGCTACGTCGGATTTGACCCTCATCTCCCCTCCCAACGCTATGACTCTTCTACCCCTTTCTCTCCCTCCGACGATATTACCTCCGCCGACCAACACCCCCATGCTCCCCCTTCCTTTTTCGAAGACGACGTCATTTCCGACATCCACACCGCTGGAAACACCACTAATCCTAATTTCTCTGACGCTTACGATTTTGGAGTTTCCAATCCTAATCCCGACTATGTATCTCCCTTTGATTCTGTTCACGCCGATGGAGACCATGACAATGCCGCCCCCGCCCCCGCCCCCGCCCCCTTCGGTGGTGGTGGTGGTGGTGGTTTTGACGATGGTGGTATTTTTGTCTCTGATGGACCTGTTCTCCCGGACCCGGGCGAAATGCGGGAGGAAGGCCATGCTCGACGAGAATGGCGACGTATTTTATGTATTATAGCTATGGGGACAGGAAAAAGGGGTATGCTTAATTCTGTCTGCTTGAAGCCTGAGTCTTTTGGAAGTTTGATGATGTGGAAAATTCTTATCTCTATTATGTATTATGTGTGTGAGCTGGCTTCATGGGCCACAGTGGTTTCATCTTTCAAGTTCCTGGGAAGTAATGGTTACCCAGCTTCCATTTTTTCTTGGAATTTCCATTTTGATCCCTGGCTAATTGTAAAGATTAGGGCTGTGATCGTTTCAAAATTTTCTGTCTTGCTGCACAAGCTGGGTCAAAGCAGTCAAAATGCCATAGATCTTGAGGAGAAAGAGAAGAAAGAAAAGGAGATGAGGAATCAAATAATTAATGAAGCTGAAGAGTATAAAGCATCCTTTTATGAGAAAAGGAGGGTCAATTGTGAAACAAATAAGGCTCACAATAGAGAAAGAGAGAAGCTTTACCATGCTAACCAGGAAAAATTTCACAAAGAAGCTGACAAACATTACTGGAAAGCAATTGCAGAGATCATCCCACGAGAGGTACCTAATATTGAGAAGAGAAGAGGAAAGAAAGATGCTGACAATAAGCCTTCAATAGTGGTTGTTCAGGGTCCAAAACCTGGAAAACCAACCGATCTGTCACGAATGAGGCAGATTCTGTTAAAGTTGAAACAGACTCCTCCACCTCATATGATGCCTCCTCCCCCCAAACCGGCCAAAGATGACAAGGATGGCAAAGATGGAAAGGATGGCAAAGATGGAAAAGATGGCAAGGATGGAAAGGATAGCAAGGAAAAGGATAGTAAGGATGGCAAGGATGGCAAAGATACAAAGGAAGGGAAGGAGGACAAGGAGGACAAAGGTGGAAAGGAAGCAAAGGAAGCAAAGGAAGGGACAGAAACCAAAGATGCTAAGGAAGAAGGGAAGGAAGTTGAGGACAAGAAAGCTTCTTCACCAGCTGCAGTTCACACACCTACATCTCCTGGAAAAGATATGGATACCAATGGTACTCCCGATCAACCCAAAATAGAGGTTGAGGCTCCTCCTGCGGCTGATACAGAACAAGCTGTTCTGTTGGTGTCACCTTCTGCAGAGTAA

Protein sequence

MASFDTFINEEHEQQHHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQHPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAAPAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRGMLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHFDPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDADNKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDGKDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDKKASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEAPPAADTEQAVLLVSPSAE
Homology
BLAST of HG10000995 vs. NCBI nr
Match: XP_008448281.1 (PREDICTED: clathrin light chain 1 [Cucumis melo])

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-170
Identity = 363/532 (68.23%), Postives = 379/532 (71.24%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKE+RNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEVRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 414

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 414

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA-PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPVADSEQAVPLVSPSAE 414

BLAST of HG10000995 vs. NCBI nr
Match: TYK04938.1 (clathrin light chain 1 [Cucumis melo var. makuwa])

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-170
Identity = 363/532 (68.23%), Postives = 379/532 (71.24%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKE+RNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEVRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 414

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 414

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA-PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPVADSEQAVPLVSPSAE 414

BLAST of HG10000995 vs. NCBI nr
Match: KAA0062081.1 (clathrin light chain 1 [Cucumis melo var. makuwa])

HSP 1 Score: 609.8 bits (1571), Expect = 2.3e-170
Identity = 363/533 (68.11%), Postives = 379/533 (71.11%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLY+ANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYYANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 415

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 415

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA--PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA  PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPPVADSEQAVPLVSPSAE 415

BLAST of HG10000995 vs. NCBI nr
Match: XP_038900435.1 (LOW QUALITY PROTEIN: clathrin light chain 1 [Benincasa hispida])

HSP 1 Score: 606.3 bits (1562), Expect = 2.5e-169
Identity = 367/534 (68.73%), Postives = 381/534 (71.35%), Query Frame = 0

Query: 1   MASFDTFINEE-HEQQHHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFDTF NEE  +QQHHHSPTAQPFDDDGYVG+D  LPS  +DSSTPFSPSDDITSADQ
Sbjct: 66  MASFDTFTNEELEQQQHHHSPTAQPFDDDGYVGYDSSLPSHPHDSSTPFSPSDDITSADQ 125

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
           HPH PPSFFEDDVIS    AG+TTNPNFSDAYDFGVSNPNPDY SPFDSVHADG+HDNAA
Sbjct: 126 HPHPPPSFFEDDVIS----AGDTTNPNFSDAYDFGVSNPNPDYASPFDSVHADGNHDNAA 185

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGGIFVSDGPVLPDP EMREEGHARREWRR             
Sbjct: 186 AA--------GGGGGFDDGGIFVSDGPVLPDPSEMREEGHARREWRR------------- 245

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKE+KEKEMRNQIINEAEEYKASFYE
Sbjct: 306 ---------------------------QNAIDLEEKEQKEKEMRNQIINEAEEYKASFYE 365

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLYHANQEKFHKEAD+HYWKAIAEIIPREVPNIEKRRGKKDAD
Sbjct: 366 KRRVNCETNKAHNREREKLYHANQEKFHKEADQHYWKAIAEIIPREVPNIEKRRGKKDAD 425

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
            KPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMM PPPKPAKD KDGK+       
Sbjct: 426 KKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMM-PPPKPAKDGKDGKE------- 475

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAK-EGTETKDAKEEGKEVED 480
           KD KDGKD++EKDS   KDGKDTKEGKEDK  K  KEAKEAK EGTETKD ++EGKEVED
Sbjct: 486 KDSKDGKDNEEKDS---KDGKDTKEGKEDKGGKEAKEAKEAKEEGTETKDTEDEGKEVED 475

Query: 481 KKASSPAAVHTPTSPGKDMDTNG--TPDQPKIEVEAPPAADTEQAVLLVSPSAE 531
           KKAS PAA HTPTSP K MDT+G  TPDQPKIEVEAPP  D +QAVLLV PSAE
Sbjct: 546 KKASPPAAGHTPTSPAKTMDTDGTLTPDQPKIEVEAPPLVDADQAVLLV-PSAE 475

BLAST of HG10000995 vs. NCBI nr
Match: XP_011656953.1 (clathrin light chain 1 [Cucumis sativus] >KAE8646860.1 hypothetical protein Csa_020622 [Cucumis sativus])

HSP 1 Score: 583.2 bits (1502), Expect = 2.3e-162
Identity = 349/535 (65.23%), Postives = 368/535 (68.79%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ---HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSA 60
           MASFDTF NEEHE +   HHHSPT QPFDDD Y+ FD  LPSQ YDSSTPFSPS DI+S 
Sbjct: 1   MASFDTFTNEEHEHEHHHHHHSPTDQPFDDDAYMAFDSTLPSQPYDSSTPFSPSHDISSP 60

Query: 61  DQHPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDN 120
           D  P  PPSFFED+VISDIH+ GNTTNPN SDAYDFGVSNPNPDYVSPF S  AD DHDN
Sbjct: 61  DHQPQPPPSFFEDNVISDIHSPGNTTNPNLSDAYDFGVSNPNPDYVSPFHSADADADHDN 120

Query: 121 AAPAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGK 180
           AA   APA     GGG FDDGG+F SDGPVLPDP EMREEG+ARREWRR           
Sbjct: 121 AA---APAV----GGGAFDDGGLFASDGPVLPDPSEMREEGNARREWRR----------- 180

Query: 181 RGMLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 HFDPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASF 300
                                        QNAIDLE+KEKKEKEMRNQIINEAEEYKASF
Sbjct: 241 -----------------------------QNAIDLEDKEKKEKEMRNQIINEAEEYKASF 300

Query: 301 YEKRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKD 360
           YEKRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD
Sbjct: 301 YEKRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKD 360

Query: 361 ADNKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGK 420
            D KPSI+VVQGPKPGKPTDL+R+RQILLKLKQTPPPHMMPPPPKPAKD+KDGKDGKDG+
Sbjct: 361 PDKKPSILVVQGPKPGKPTDLARLRQILLKLKQTPPPHMMPPPPKPAKDEKDGKDGKDGE 411

Query: 421 DGKDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVE 480
            GKDG           KD KDGKDTKEG    E+KGG   KEA EGTETKD+KEEGKEV+
Sbjct: 421 GGKDG-----------KDSKDGKDTKEG---TENKGG---KEASEGTETKDSKEEGKEVD 411

Query: 481 DKKASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA--PPAADTEQAVLLVSPSAE 531
           +KKASSPA V TPTSP  +MD NGTPDQP IEVEA  PP AD EQAVLLVSPSAE
Sbjct: 481 EKKASSPATVLTPTSPAINMDANGTPDQPIIEVEAPPPPVADAEQAVLLVSPSAE 411

BLAST of HG10000995 vs. ExPASy Swiss-Prot
Match: Q9SKU1 (Clathrin light chain 1 OS=Arabidopsis thaliana OX=3702 GN=At2g20760 PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 3.1e-69
Identity = 203/452 (44.91%), Postives = 231/452 (51.11%), Query Frame = 0

Query: 27  DDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQHPHAPPSFFEDDVISDIHTAGNTTNPN 86
           DDG      H PS+  D    F   D+ + A Q    PP+  +    S  +  G+  +PN
Sbjct: 5   DDGDFPAQTHSPSEHED----FGGYDNFSEAQQ----PPTQHQSGGFSSFN--GDPASPN 64

Query: 87  FSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAAPAPAPAPFGGGGGGGFDDGGIFVSDGP 146
               Y FG S+PN D+ SPF+S   D +             G GGG G D   IF SDGP
Sbjct: 65  ---GYGFGASSPNHDFSSPFESSVNDAN-------------GNGGGSGGD--AIFASDGP 124

Query: 147 VLPDPGEMREEGHARREWRRILCIIAMGTGKRGMLNSVCLKPESFGSLMMWKILISIMYY 206
           +LPDP EMREEG  RREWRR+                                       
Sbjct: 125 ILPDPNEMREEGFQRREWRRL--------------------------------------- 184

Query: 207 VCELASWATVVSSFKFLGSNGYPASIFSWNFHFDPWLIVKIRAVIVSKFSVLLHKLGQSS 266
                                                                       
Sbjct: 185 ------------------------------------------------------------ 244

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
            N I LEEKEKKEKEMRNQII EAE++K +FYEKR    ETNK  NRE+EKLY ANQEKF
Sbjct: 245 -NTIHLEEKEKKEKEMRNQIITEAEDFKKAFYEKRDKTIETNKTDNREKEKLYWANQEKF 304

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDADNKPSIVVVQGPKPGKPTDLSRMRQILL 386
           HKE DKHYWKAIAE+IPREVPNIEK+RGKKD D KPS+ V+QGPKPGKPTDL RMRQI L
Sbjct: 305 HKEVDKHYWKAIAELIPREVPNIEKKRGKKDPDKKPSVNVIQGPKPGKPTDLGRMRQIFL 322

Query: 387 KLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDGKDGKDGKDSKEKDSKDGKDGKDTKEGK 446
           KLK  PPPHMMPPPP PAKD KDGKD KDGKD K GKDG     KD+K GKD KD K+GK
Sbjct: 365 KLKTNPPPHMMPPPP-PAKDAKDGKDAKDGKDAKTGKDG-----KDAKGGKDAKDLKDGK 322

Query: 447 ----EDKEDKGGKEAKEAKEGTETKDAKEEGK 475
               +  E+K    AK+A   T   DA   G+
Sbjct: 425 PADPKVTEEKRPSPAKDASVETAKPDAAASGE 322

BLAST of HG10000995 vs. ExPASy Swiss-Prot
Match: Q5Z402 (Clathrin light chain 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0731800 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.2e-50
Identity = 111/152 (73.03%), Postives = 123/152 (80.92%), Query Frame = 0

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
           QNAI LEEKEKKEKEMRNQII +A+E+K +F EKR++N ET+K  NREREKLY ANQEKF
Sbjct: 100 QNAILLEEKEKKEKEMRNQIILDAKEFKKAFVEKRKLNVETSKDQNREREKLYLANQEKF 159

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD--NKPSIVVVQGPKPGKPTDLSRMRQI 386
           H  ADK YWKAI+E+IP E+ NIEKR  KKD D   KP IVV+QGPKPGKPTD+SRMRQI
Sbjct: 160 HAGADKQYWKAISELIPHEIANIEKRGAKKDKDKEKKPGIVVIQGPKPGKPTDMSRMRQI 219

Query: 387 LLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDG 417
           LLKLK TPPPHM PPPP  A   KDG  GKDG
Sbjct: 220 LLKLKHTPPPHMKPPPPPAAATGKDGAAGKDG 251

BLAST of HG10000995 vs. ExPASy Swiss-Prot
Match: Q7XKE9 (Clathrin light chain 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0679100 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-43
Identity = 113/183 (61.75%), Postives = 132/183 (72.13%), Query Frame = 0

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
           QNAI LEEKE+KEKE+R QI+ EAEE+K +FYEKR  NCETNK HNREREK++ A QEKF
Sbjct: 118 QNAIVLEEKERKEKELRAQILAEAEEFKKAFYEKRIQNCETNKVHNREREKIFVAGQEKF 177

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDADNKPSIVVVQGPKPGKPTDLSRMRQILL 386
           H EADK YWK+I+E+IP E+  IEK RGKKD D KPSI V+QGPKPGKPTDLSRMRQIL+
Sbjct: 178 HAEADKQYWKSISELIPHEIATIEK-RGKKDKDKKPSITVIQGPKPGKPTDLSRMRQILV 237

Query: 387 KLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDGKDGKDGKDSKEKDSKDGKDGKDTKEGK 446
           KLK  PPPHMM PPP  A  D   KDG   KDG         K  +SK+        E +
Sbjct: 238 KLKHAPPPHMMQPPPASAAKD-GAKDG--AKDGTPAPANGTKKPAESKEKPANGSPAEAE 296

Query: 447 EDK 450
           +++
Sbjct: 298 KEQ 296

BLAST of HG10000995 vs. ExPASy Swiss-Prot
Match: Q6Z3A8 (Clathrin light chain 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0461500 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 5.2e-40
Identity = 106/187 (56.68%), Postives = 134/187 (71.66%), Query Frame = 0

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
           QNA+ LEEKE+KE+E R +II EA+E+K SF EKR++N +TN+A NR+REKL+ A QEKF
Sbjct: 158 QNALMLEEKERKERERRGEIIAEADEFKRSFAEKRKLNGDTNRAQNRDREKLFLAKQEKF 217

Query: 327 HKEADKHYWKAIAEIIPREVPNIE---KRRGKKDAD-----NKPSIVVVQGPKPGKPTDL 386
           H EA+K YWKAIAE++P E+P +E   KRR K+ A+      +P +VVVQG KPGKPTDL
Sbjct: 218 HGEAEKQYWKAIAEMVPHEIPGLEKRGKRREKQSAEANAKAKQPGVVVVQGTKPGKPTDL 277

Query: 387 SRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDGKDGKDGKDSKEKDSKDGKD 446
           SRMRQ+L+KLKQTPPPHM PPPP+PAKD      G D    KDG+  K + E + K    
Sbjct: 278 SRMRQVLMKLKQTPPPHMAPPPPQPAKD-----TGGDTDANKDGEAEKAAGEIEKKAAGG 337

BLAST of HG10000995 vs. ExPASy Swiss-Prot
Match: O04209 (Clathrin light chain 2 OS=Arabidopsis thaliana OX=3702 GN=CLC2 PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.6e-39
Identity = 92/154 (59.74%), Postives = 116/154 (75.32%), Query Frame = 0

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
           QNAI LEEKEK+EKE+  QII EA++YK  F++K  V CE NKA NRE+EKLY  NQEKF
Sbjct: 97  QNAIQLEEKEKREKELLKQIIEEADQYKEEFHKKIEVTCENNKAANREKEKLYLENQEKF 156

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDADN--KPSIVVVQGPKPGKPTDLSRMRQI 386
           + E+ K+YWKAIAE++P+EVP IEKRRGKK+  +  KP++ V+QGPKPGKPTDL+RMRQI
Sbjct: 157 YAESSKNYWKAIAELVPKEVPTIEKRRGKKEQQDPKKPTVSVIQGPKPGKPTDLTRMRQI 216

Query: 387 LLKLKQTPPPHM----MPPPPKPAKDDKDGKDGK 415
           L+KLK  PP H+     PP  + A   K+  + K
Sbjct: 217 LVKLKHNPPSHLKLTSQPPSEEAAAPPKNVPETK 250

BLAST of HG10000995 vs. ExPASy TrEMBL
Match: A0A5D3C0Z6 (Clathrin light chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001510 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 5.0e-171
Identity = 363/532 (68.23%), Postives = 379/532 (71.24%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKE+RNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEVRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 414

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 414

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA-PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPVADSEQAVPLVSPSAE 414

BLAST of HG10000995 vs. ExPASy TrEMBL
Match: A0A1S3BJB2 (Clathrin light chain OS=Cucumis melo OX=3656 GN=LOC103490517 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 5.0e-171
Identity = 363/532 (68.23%), Postives = 379/532 (71.24%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKE+RNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEVRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 414

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 414

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA-PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPVADSEQAVPLVSPSAE 414

BLAST of HG10000995 vs. ExPASy TrEMBL
Match: A0A5A7V8R6 (Clathrin light chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G004290 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 1.1e-170
Identity = 363/533 (68.11%), Postives = 379/533 (71.11%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ-HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQ 60
           MASFD F NEEHEQQ HHHSPTAQPFDDDGY+G+D  LPSQ YDSSTPFSPS D++ A+Q
Sbjct: 1   MASFDAFTNEEHEQQHHHHSPTAQPFDDDGYMGYDSRLPSQPYDSSTPFSPSHDVSPAEQ 60

Query: 61  HPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAA 120
            P APPSFFEDDVISDIHT GNTT PN SDAYDFGVSNPNPDYVSPFDSV AD DHDNAA
Sbjct: 61  QPQAPPSFFEDDVISDIHTPGNTTTPNLSDAYDFGVSNPNPDYVSPFDSVDADADHDNAA 120

Query: 121 PAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRG 180
            A        GGGGGFDDGG+FVSDGPVLPDP EMREEG+ARREWRR             
Sbjct: 121 AAAV------GGGGGFDDGGLFVSDGPVLPDPSEMREEGNARREWRR------------- 180

Query: 181 MLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 DPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300
                                      QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE
Sbjct: 241 ---------------------------QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYE 300

Query: 301 KRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDAD 360
           KRRVNCETNKAHNREREKLY+ANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD D
Sbjct: 301 KRRVNCETNKAHNREREKLYYANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDPD 360

Query: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDG 420
           NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPK AKD+KDGKDGKDG+DG
Sbjct: 361 NKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKLAKDNKDGKDGKDGEDG 415

Query: 421 KDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVEDK 480
           KDGKD K+      KD KDGKDTKEG   KEDKGG    EA  GT TKDAKEEGKEV++K
Sbjct: 421 KDGKDSKE------KDSKDGKDTKEG---KEDKGG---NEATVGTGTKDAKEEGKEVDEK 415

Query: 481 KASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA--PPAADTEQAVLLVSPSAE 531
           KASSPAAV TPTSP  +MD NGTPDQP IEVEA  PP AD+EQAV LVSPSAE
Sbjct: 481 KASSPAAVLTPTSPAINMDANGTPDQPIIEVEAPPPPVADSEQAVPLVSPSAE 415

BLAST of HG10000995 vs. ExPASy TrEMBL
Match: A0A0A0KD26 (Clathrin light chain OS=Cucumis sativus OX=3659 GN=Csa_6G128590 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 1.1e-162
Identity = 349/535 (65.23%), Postives = 368/535 (68.79%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQ---HHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSA 60
           MASFDTF NEEHE +   HHHSPT QPFDDD Y+ FD  LPSQ YDSSTPFSPS DI+S 
Sbjct: 1   MASFDTFTNEEHEHEHHHHHHSPTDQPFDDDAYMAFDSTLPSQPYDSSTPFSPSHDISSP 60

Query: 61  DQHPHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDN 120
           D  P  PPSFFED+VISDIH+ GNTTNPN SDAYDFGVSNPNPDYVSPF S  AD DHDN
Sbjct: 61  DHQPQPPPSFFEDNVISDIHSPGNTTNPNLSDAYDFGVSNPNPDYVSPFHSADADADHDN 120

Query: 121 AAPAPAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGK 180
           AA   APA     GGG FDDGG+F SDGPVLPDP EMREEG+ARREWRR           
Sbjct: 121 AA---APAV----GGGAFDDGGLFASDGPVLPDPSEMREEGNARREWRR----------- 180

Query: 181 RGMLNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNF 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 HFDPWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASF 300
                                        QNAIDLE+KEKKEKEMRNQIINEAEEYKASF
Sbjct: 241 -----------------------------QNAIDLEDKEKKEKEMRNQIINEAEEYKASF 300

Query: 301 YEKRRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKD 360
           YEKRRVNCETNKAHNREREKLYHANQE+FHKEADKHYWKAIAEIIPREVPNIEKRRGKKD
Sbjct: 301 YEKRRVNCETNKAHNREREKLYHANQERFHKEADKHYWKAIAEIIPREVPNIEKRRGKKD 360

Query: 361 ADNKPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKPAKDDKDGKDGKDGK 420
            D KPSI+VVQGPKPGKPTDL+R+RQILLKLKQTPPPHMMPPPPKPAKD+KDGKDGKDG+
Sbjct: 361 PDKKPSILVVQGPKPGKPTDLARLRQILLKLKQTPPPHMMPPPPKPAKDEKDGKDGKDGE 411

Query: 421 DGKDGKDGKDSKEKDSKDGKDGKDTKEGKEDKEDKGGKEAKEAKEGTETKDAKEEGKEVE 480
            GKDG           KD KDGKDTKEG    E+KGG   KEA EGTETKD+KEEGKEV+
Sbjct: 421 GGKDG-----------KDSKDGKDTKEG---TENKGG---KEASEGTETKDSKEEGKEVD 411

Query: 481 DKKASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEA--PPAADTEQAVLLVSPSAE 531
           +KKASSPA V TPTSP  +MD NGTPDQP IEVEA  PP AD EQAVLLVSPSAE
Sbjct: 481 EKKASSPATVLTPTSPAINMDANGTPDQPIIEVEAPPPPVADAEQAVLLVSPSAE 411

BLAST of HG10000995 vs. ExPASy TrEMBL
Match: A0A6J1G701 (Clathrin light chain OS=Cucurbita moschata OX=3662 GN=LOC111451437 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 3.7e-158
Identity = 339/536 (63.25%), Postives = 364/536 (67.91%), Query Frame = 0

Query: 1   MASFDTFINEEHEQQHHHSPTAQPFDDDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQH 60
           MAS+DTF N+EH++Q   SPT++PFDDDGY+G+D H PSQ +DS   FSP     S DQH
Sbjct: 1   MASYDTFSNDEHQEQQQQSPTSRPFDDDGYIGYDTHHPSQSFDSLATFSP-----STDQH 60

Query: 61  PHAPPSFFEDDVISDIHTAGNTTNPNFSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAAP 120
           PH PPSFFEDDVISDIH+AGNT NPN SD YDFGVSNPNP YVSPFD V  D D+ NAA 
Sbjct: 61  PHPPPSFFEDDVISDIHSAGNTNNPNLSDVYDFGVSNPNPGYVSPFDPVETDVDNGNAA- 120

Query: 121 APAPAPFGGGGGGGFDDGGIFVSDGPVLPDPGEMREEGHARREWRRILCIIAMGTGKRGM 180
                      G G  D G F SDGPVLPDP EMREE HARREWRR              
Sbjct: 121 ----------AGIGVIDDGFFASDGPVLPDPSEMREECHARREWRR-------------- 180

Query: 181 LNSVCLKPESFGSLMMWKILISIMYYVCELASWATVVSSFKFLGSNGYPASIFSWNFHFD 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 PWLIVKIRAVIVSKFSVLLHKLGQSSQNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEK 300
                                     QNAIDL EKEKKEKEMRNQIINEAEEYKASFYEK
Sbjct: 241 --------------------------QNAIDLAEKEKKEKEMRNQIINEAEEYKASFYEK 300

Query: 301 RRVNCETNKAHNREREKLYHANQEKFHKEADKHYWKAIAEIIPREVPNIEKRRGKKDADN 360
           RR+NCETNKAHNREREKLYH NQEKFHKEADKH+WKAIAEIIPREVPNIEKRRGKKDA+N
Sbjct: 301 RRINCETNKAHNREREKLYHTNQEKFHKEADKHFWKAIAEIIPREVPNIEKRRGKKDAEN 360

Query: 361 KPSIVVVQGPKPGKPTDLSRMRQILLKLKQTPPPHMMPPPPKP-AKDDKDGKDGKDGKDG 420
           KPSIV+VQGPKPGKPTDL+RMRQILLKLKQTPPPHMMPPPPKP  KD KDGKDGKD KDG
Sbjct: 361 KPSIVIVQGPKPGKPTDLARMRQILLKLKQTPPPHMMPPPPKPDDKDAKDGKDGKDSKDG 418

Query: 421 KDGKDGKDSKE-KDSKDGKDGKDTKEGKEDKEDKGGKEAKE------AKEGTETKDAKEE 480
           KDGKDGKD K+ KD KDGKDGKD KEGKE  EDKGGKEAKE      AKEGTETKDAKE 
Sbjct: 421 KDGKDGKDGKDGKDGKDGKDGKDKKEGKE--EDKGGKEAKEGTETKDAKEGTETKDAKEG 418

Query: 481 GKEVEDKKASSPAAVHTPTSPGKDMDTNGTPDQPKIEVEAPPAADTEQAVLLVSPS 529
           GK+VEDKK+SSPAA H+P SP KD+D NGTP+QPKIE+EAP AAD EQ +LLVSP+
Sbjct: 481 GKKVEDKKSSSPAAAHSPRSPAKDVDANGTPEQPKIEIEAPTAADAEQPILLVSPA 418

BLAST of HG10000995 vs. TAIR 10
Match: AT2G20760.1 (Clathrin light chain protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.2e-70
Identity = 203/452 (44.91%), Postives = 231/452 (51.11%), Query Frame = 0

Query: 27  DDGYVGFDPHLPSQRYDSSTPFSPSDDITSADQHPHAPPSFFEDDVISDIHTAGNTTNPN 86
           DDG      H PS+  D    F   D+ + A Q    PP+  +    S  +  G+  +PN
Sbjct: 5   DDGDFPAQTHSPSEHED----FGGYDNFSEAQQ----PPTQHQSGGFSSFN--GDPASPN 64

Query: 87  FSDAYDFGVSNPNPDYVSPFDSVHADGDHDNAAPAPAPAPFGGGGGGGFDDGGIFVSDGP 146
               Y FG S+PN D+ SPF+S   D +             G GGG G D   IF SDGP
Sbjct: 65  ---GYGFGASSPNHDFSSPFESSVNDAN-------------GNGGGSGGD--AIFASDGP 124

Query: 147 VLPDPGEMREEGHARREWRRILCIIAMGTGKRGMLNSVCLKPESFGSLMMWKILISIMYY 206
           +LPDP EMREEG  RREWRR+                                       
Sbjct: 125 ILPDPNEMREEGFQRREWRRL--------------------------------------- 184

Query: 207 VCELASWATVVSSFKFLGSNGYPASIFSWNFHFDPWLIVKIRAVIVSKFSVLLHKLGQSS 266
                                                                       
Sbjct: 185 ------------------------------------------------------------ 244

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
            N I LEEKEKKEKEMRNQII EAE++K +FYEKR    ETNK  NRE+EKLY ANQEKF
Sbjct: 245 -NTIHLEEKEKKEKEMRNQIITEAEDFKKAFYEKRDKTIETNKTDNREKEKLYWANQEKF 304

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDADNKPSIVVVQGPKPGKPTDLSRMRQILL 386
           HKE DKHYWKAIAE+IPREVPNIEK+RGKKD D KPS+ V+QGPKPGKPTDL RMRQI L
Sbjct: 305 HKEVDKHYWKAIAELIPREVPNIEKKRGKKDPDKKPSVNVIQGPKPGKPTDLGRMRQIFL 322

Query: 387 KLKQTPPPHMMPPPPKPAKDDKDGKDGKDGKDGKDGKDGKDSKEKDSKDGKDGKDTKEGK 446
           KLK  PPPHMMPPPP PAKD KDGKD KDGKD K GKDG     KD+K GKD KD K+GK
Sbjct: 365 KLKTNPPPHMMPPPP-PAKDAKDGKDAKDGKDAKTGKDG-----KDAKGGKDAKDLKDGK 322

Query: 447 ----EDKEDKGGKEAKEAKEGTETKDAKEEGK 475
               +  E+K    AK+A   T   DA   G+
Sbjct: 425 PADPKVTEEKRPSPAKDASVETAKPDAAASGE 322

BLAST of HG10000995 vs. TAIR 10
Match: AT2G40060.1 (Clathrin light chain protein )

HSP 1 Score: 164.9 bits (416), Expect = 1.8e-40
Identity = 92/154 (59.74%), Postives = 116/154 (75.32%), Query Frame = 0

Query: 267 QNAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKF 326
           QNAI LEEKEK+EKE+  QII EA++YK  F++K  V CE NKA NRE+EKLY  NQEKF
Sbjct: 97  QNAIQLEEKEKREKELLKQIIEEADQYKEEFHKKIEVTCENNKAANREKEKLYLENQEKF 156

Query: 327 HKEADKHYWKAIAEIIPREVPNIEKRRGKKDADN--KPSIVVVQGPKPGKPTDLSRMRQI 386
           + E+ K+YWKAIAE++P+EVP IEKRRGKK+  +  KP++ V+QGPKPGKPTDL+RMRQI
Sbjct: 157 YAESSKNYWKAIAELVPKEVPTIEKRRGKKEQQDPKKPTVSVIQGPKPGKPTDLTRMRQI 216

Query: 387 LLKLKQTPPPHM----MPPPPKPAKDDKDGKDGK 415
           L+KLK  PP H+     PP  + A   K+  + K
Sbjct: 217 LVKLKHNPPSHLKLTSQPPSEEAAAPPKNVPETK 250

BLAST of HG10000995 vs. TAIR 10
Match: AT3G51890.1 (Clathrin light chain protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-37
Identity = 88/140 (62.86%), Postives = 101/140 (72.14%), Query Frame = 0

Query: 268 NAIDLEEKEKKEKEMRNQIINEAEEYKASFYEKRRVNCETNKAHNREREKLYHANQEKFH 327
           NA+ LEEKEK+EKEM  QI+  AE+YKA FY KR V  E NK  NRE+EK +  NQEKF+
Sbjct: 107 NALRLEEKEKEEKEMVQQILEAAEQYKAEFYSKRNVTIENNKKLNREKEKFFLENQEKFY 166

Query: 328 KEADKHYWKAIAEIIPREVPNIEKRRGKKDADNKPSIVVVQGPKPGKPTDLSRMRQILLK 387
            EADK+ WKAIAE+IPREVP IE R  KK      +I V+QGPKPGKPTDLSRMRQ+L K
Sbjct: 167 AEADKNNWKAIAELIPREVPVIENRGNKK---KTATITVIQGPKPGKPTDLSRMRQVLTK 226

Query: 388 LKQTPPPHMMPPPPKPAKDD 408
           LK  PP HM P  P P+  D
Sbjct: 227 LKHNPPTHMKPKLPSPSGAD 243

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008448281.11.0e-17068.23PREDICTED: clathrin light chain 1 [Cucumis melo][more]
TYK04938.11.0e-17068.23clathrin light chain 1 [Cucumis melo var. makuwa][more]
KAA0062081.12.3e-17068.11clathrin light chain 1 [Cucumis melo var. makuwa][more]
XP_038900435.12.5e-16968.73LOW QUALITY PROTEIN: clathrin light chain 1 [Benincasa hispida][more]
XP_011656953.12.3e-16265.23clathrin light chain 1 [Cucumis sativus] >KAE8646860.1 hypothetical protein Csa_... [more]
Match NameE-valueIdentityDescription
Q9SKU13.1e-6944.91Clathrin light chain 1 OS=Arabidopsis thaliana OX=3702 GN=At2g20760 PE=2 SV=1[more]
Q5Z4024.2e-5073.03Clathrin light chain 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0731800 ... [more]
Q7XKE91.7e-4361.75Clathrin light chain 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0679100 ... [more]
Q6Z3A85.2e-4056.68Clathrin light chain 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0461500 ... [more]
O042092.6e-3959.74Clathrin light chain 2 OS=Arabidopsis thaliana OX=3702 GN=CLC2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3C0Z65.0e-17168.23Clathrin light chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143... [more]
A0A1S3BJB25.0e-17168.23Clathrin light chain OS=Cucumis melo OX=3656 GN=LOC103490517 PE=3 SV=1[more]
A0A5A7V8R61.1e-17068.11Clathrin light chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G... [more]
A0A0A0KD261.1e-16265.23Clathrin light chain OS=Cucumis sativus OX=3659 GN=Csa_6G128590 PE=3 SV=1[more]
A0A6J1G7013.7e-15863.25Clathrin light chain OS=Cucurbita moschata OX=3662 GN=LOC111451437 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G20760.12.2e-7044.91Clathrin light chain protein [more]
AT2G40060.11.8e-4059.74Clathrin light chain protein [more]
AT3G51890.11.4e-3762.86Clathrin light chain protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 270..292
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 485..499
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 111..130
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 401..482
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 349..530
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..69
NoneNo IPR availablePANTHERPTHR10639:SF33CLATHRIN LIGHT CHAINcoord: 1..167
NoneNo IPR availablePANTHERPTHR10639:SF33CLATHRIN LIGHT CHAINcoord: 267..433
IPR000996Clathrin light chainPANTHERPTHR10639CLATHRIN LIGHT CHAINcoord: 1..167
IPR000996Clathrin light chainPANTHERPTHR10639CLATHRIN LIGHT CHAINcoord: 267..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000995.1HG10000995.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0030132 clathrin coat of coated pit
cellular_component GO:0030130 clathrin coat of trans-Golgi network vesicle
molecular_function GO:0005198 structural molecule activity