HG10000962 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000962
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionvesicle-associated protein 4-2-like
LocationChr09: 12567046 .. 12569300 (+)
RNA-Seq ExpressionHG10000962
SyntenyHG10000962
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATGTATGTCTCCGGTGGCTCTTTTCTCTTGTGCTTGGTTGATTTTCTGCCTCGTGGGGTACTTGGTTTTTGGTTTATTTATAGGGTTTAGTGTTTCCTTGATTAAGGTAGTTGATTGTTTCATCTGTGTCGTCATGTTTCTGGGTTCATAATGAGAATTTTGTTGCCTTTTCGTTCTGTTCTTTAACTGTGTTTGGTTTCTGAATGACAGAATGTCTTTTTTGCTTCTTTCCTCTTTATCAATGTGGAAGCTCTAATTTTTGGTTTCTTGCTTTTCTGATCCGGCAATGAACTGAGTTAAAAATGAATTTTAATGTGTTGATTTAGTTATCCTCCCCTGTTCCTTGGTGAAGCTTACAAAAGGATGACGATTGATGACTACTTAGTTACTCTCTAACAGATGTTGTCTGAGTTTAAAACTCATGCACCCAAAAAGGATATGTTCTTGTTTGGGTTTATAGAATGTGTTTTTTCTTTTCTTTTTGTTCATTTTTTCAGATCGCTTCCTCTCTATTTGGTTATATAGGATAGTTTGTTTCGTCGTCTAACAAGAATGGGGCCTTTGAATTGCATATATTACTTGTTGTATCATTTTAGTTGTTGACTAGATTTGTTTTTCCTCACAACTGTGTTTTGATTTCATTCTTTCATTTCTTCTTTCCCCTTAAAGTTGAACCTGGGAAGCAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAAGTATGGATTATTTCCCTCTCGAACTTTTATCAGTATGAATACTGAGTTGTTTAGTTTTATTATAGATGCCAAACTACCTTTGTTATATTAATCTACCCAATGCAGCCATCTTACTTTTGATCCCTTTAATTGTTGTAGTTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGGTAAGACATTTGTCTGGCATTTGATCTGTATCCTTAGAATTCAACATTGCAAGTAGAAACAATGTAATTAAATCATTTGGAATTTTGCTGAGGTTCCCATATCTGTGACTTGACTTCAGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGGTATGTCTTGCAATCTCAAATTCTGAAATAGTTACTGTTTTTCCATGCTTAGAGTTTGTAACCAATGCTTGTATCATTATTTCTCAAGATGAGTATTTACTATTTCAATGATCTATGAAGATTAATGTCTGCTGACATTTCAAGATAACTTCCTGAGATATAGTTTTGTTGCATTCACAATGACGAAAATGAATGTTAGTCTAATTAAGTACTTTTAGTTGCATATTTATCAATCCATGATGCAATCTTCTAGTTTTCTTATGTGAAAGCCTAATTATAATTCAGTCTAGTATTATAACTTTCTGATGGTAATTTCAAATAATGGCATTTTACAGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGTAAGTAGTAACAGGAGATAAACACAATATCAAGTACAGCCATGATTGCTTCTCGCATCAATCTTCTTATCGGTTCGGATTCTTCTCCTTTATTCTTTTTCAGGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGGTGAGATAACTAGACTGTTGTCCACATCTTCATCAAACCTTACTGAGTTTATTTGAACATTTACATTCTCTGACATACTTGATGCTATGGTTCTTCATTTGTAGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAG

mRNA sequence

ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATTTGAACCTGGGAAGCAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAATTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAG

Coding sequence (CDS)

ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATTTGAACCTGGGAAGCAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAATTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAG

Protein sequence

MAVANEKSGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHSSSHPGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV
Homology
BLAST of HG10000962 vs. NCBI nr
Match: XP_011656923.2 (vesicle-associated protein 4-2 [Cucumis sativus] >KGN46696.2 hypothetical protein Csa_020729 [Cucumis sativus])

HSP 1 Score: 479.2 bits (1232), Expect = 2.3e-131
Identity = 242/268 (90.30%), Postives = 248/268 (92.54%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP------SSASSSSLIHRVHLQSHSHKPAEHS 60
           MAVA+EKSG D KVW+LCSIPFWQNSNAP      SS+SSSS+IHRVHLQS+ HKPAEHS
Sbjct: 1   MAVADEKSGPDGKVWSLCSIPFWQNSNAPSSSSSSSSSSSSSVIHRVHLQSNGHKPAEHS 60

Query: 61  SSHPGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTT 120
           SSHPGVVSAIAKSL PTKRRLRLDPSN LYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTT
Sbjct: 61  SSHPGVVSAIAKSLCPTKRRLRLDPSNNLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTT 120

Query: 121 APKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYV 180
           APKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK D+DYV
Sbjct: 121 APKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDLDYV 180

Query: 181 PELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIV 240
           PELFDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIV
Sbjct: 181 PELFDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIV 240

Query: 241 GGGLVLDEWKERRERYLAKQQVGGVGLV 263
           GGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 GGGLVLDEWKERRERYLAKQQVGGVDLV 268

BLAST of HG10000962 vs. NCBI nr
Match: XP_038900695.1 (vesicle-associated protein 4-2-like [Benincasa hispida])

HSP 1 Score: 477.2 bits (1227), Expect = 8.8e-131
Identity = 239/253 (94.47%), Postives = 246/253 (97.23%), Query Frame = 0

Query: 11  DEKVWTLCSIPFWQNSNAP-SSASSSSLIHRVHLQSHSHKPAEHSSSHPGVVSAIAKSLL 70
           DEKV TLCSIPFWQNSNAP SS+SSSS+IHRVHLQS+ HKPAEHSSSHPGVVS+IAKSLL
Sbjct: 5   DEKVLTLCSIPFWQNSNAPSSSSSSSSVIHRVHLQSNGHKPAEHSSSHPGVVSSIAKSLL 64

Query: 71  PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILA 130
           PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILA
Sbjct: 65  PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILA 124

Query: 131 PDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQ 190
           PDESIIATVFKFVEPLDNNDKHVDHK+RVKFKIVSLKVKG+MDYVPELFDEQ+DHVAVEQ
Sbjct: 125 PDESIIATVFKFVEPLDNNDKHVDHKKRVKFKIVSLKVKGNMDYVPELFDEQRDHVAVEQ 184

Query: 191 ILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRER 250
           ILQVVFLDPDHSNPALEKVFRQLAEAEAA E RQKPAEE DPKIVGGGLVLDEWKERRER
Sbjct: 185 ILQVVFLDPDHSNPALEKVFRQLAEAEAATEMRQKPAEETDPKIVGGGLVLDEWKERRER 244

Query: 251 YLAKQQVGGVGLV 263
           YLAKQQVGGV LV
Sbjct: 245 YLAKQQVGGVDLV 257

BLAST of HG10000962 vs. NCBI nr
Match: XP_008448221.1 (PREDICTED: vesicle-associated protein 4-2-like [Cucumis melo] >TYK02188.1 vesicle-associated protein 4-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 476.1 bits (1224), Expect = 2.0e-130
Identity = 241/265 (90.94%), Postives = 246/265 (92.83%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120

Query: 121 SCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 180
           SCYMRPPGGILAPDES IATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK DMDYVPEL
Sbjct: 121 SCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPEL 180

Query: 181 FDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGG 240
           FDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGGG
Sbjct: 181 FDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGGG 240

Query: 241 LVLDEWKERRERYLAKQQVGGVGLV 263
           LVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 LVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of HG10000962 vs. NCBI nr
Match: KAA0047916.1 (vesicle-associated protein 4-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 473.8 bits (1218), Expect = 9.7e-130
Identity = 240/265 (90.57%), Postives = 245/265 (92.45%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSK HVAFKFQTTAPK
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKTHVAFKFQTTAPK 120

Query: 121 SCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 180
           SCYMRPPGGILAPDES IATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK DMDYVPEL
Sbjct: 121 SCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPEL 180

Query: 181 FDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGG 240
           FDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGGG
Sbjct: 181 FDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGGG 240

Query: 241 LVLDEWKERRERYLAKQQVGGVGLV 263
           LVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 LVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of HG10000962 vs. NCBI nr
Match: XP_022970681.1 (vesicle-associated protein 4-2-like [Cucurbita maxima])

HSP 1 Score: 438.0 bits (1125), Expect = 5.9e-119
Identity = 222/264 (84.09%), Postives = 241/264 (91.29%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSN-APSSASSSSLIHRVHLQSHSHKPAEHSSSHP- 60
           MAVA++KS  D KVW+LCSIPFWQN N + SS SSS+ IHRV+ QS+ HKPAE+S S+P 
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKS 120
           G VS IAKSLLPT+RRLRLDPSNKLYFP+EPGKQVKSAIEI+NTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELF 180
           CYMRPPGGILAP ESIIATVFKFVEPL+NN+K VDHKRRVKFKIVSLKVKG+MDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDHKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGL 240
           DEQ+DHVAVEQILQVVFLDP HSNPALEK+FRQLA AEAA E RQKPAEE DPK+VG GL
Sbjct: 181 DEQRDHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VLDEWKERRERYLAKQQVGGVGLV 263
           V+DEWKERRERYLAKQQVGGV LV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of HG10000962 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 5.6e-88
Identity = 170/272 (62.50%), Postives = 203/272 (74.63%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFK 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP+EPGKQV+SAI+IKNTSK+HVAFK
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFK 146

Query: 128 FQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGD 187
           FQTTAPKSC+MRPPG ILAP E+IIATVFKFVEP +NN+K +D + RVKFKI+SLKVKG 
Sbjct: 147 FQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGP 206

Query: 188 MDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEAD 247
           MDYVPELFDEQKD V+ EQIL+V+FLDP+ SNPALEK+ RQLAEA+AA+E R+KP EE  
Sbjct: 207 MDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETG 266

Query: 248 PKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 263
           PK++G GLV+DEWKERRERYLA+QQ  G   V
Sbjct: 267 PKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of HG10000962 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 4.8e-79
Identity = 151/251 (60.16%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 12  EKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHS--SSHPGVVSAIAKSLL 71
           + ++ LC  PFWQ  +  SS+S+ +  ++ +   H ++  + S  S  P  +S++A+SLL
Sbjct: 15  KNLFRLC--PFWQRRSTTSSSSTQN-PNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 74

Query: 72  PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILA 131
           P +RRLRLDPS+ LYFP+EPGKQV+SAI++KNTSK+H AFKFQTTAPKSCYMRPPGG+LA
Sbjct: 75  PARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLA 134

Query: 132 PDESIIATVFKFVEPLDNNDKH-VDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVE 191
           P ES+ ATVFKFVE  +NN+K  ++ K +VKFKI+SLKVK  ++YVPELFDEQKD VAVE
Sbjct: 135 PGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVE 194

Query: 192 QILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRE 251
           Q+L+V+F+D D  + ALEK+ RQL EAEAA+E R+KP  E  P++VG GLV+DEWKERRE
Sbjct: 195 QVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERRE 254

Query: 252 RYLAKQQVGGV 260
           +YLA+QQV  V
Sbjct: 255 KYLARQQVESV 262

BLAST of HG10000962 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 3.4e-77
Identity = 161/285 (56.49%), Postives = 204/285 (71.58%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFW-QNSNAPSSASSSSL------IHRVHL-QSHSHKPAE 60
           MA+  +KS  D + W    +PF   NS APS++SSSS+      +   HL Q++ H+   
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 61  HSSSHPGVV-----------------SAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKS 120
                P VV                 S++A+SLLPTKRRL+LDPS KLYFP+EPGKQV+S
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQVRS 120

Query: 121 AIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHK 180
           AI+IKNTSK+HVAFKFQTT PKSC+MRP G ILAP E IIATVFKFVEP +NN+K ++ K
Sbjct: 121 AIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPMEQK 180

Query: 181 RRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEA 240
             VKFKI+SLK+K   DY+PELF+EQKDHV+ EQ+++VVFLDP++ N  +EK+  QLAEA
Sbjct: 181 SGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQLAEA 240

Query: 241 EAAIETRQKPAEE-ADPKIVGGGLVLDEWKERRERYLAKQQVGGV 260
           +AA E R+K +E    PK +G GLV+DEWK+RRERYLA+QQ GGV
Sbjct: 241 DAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGV 284

BLAST of HG10000962 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-06
Identity = 34/143 (23.78%), Postives = 63/143 (44.06%), Query Frame = 0

Query: 85  FPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEP 144
           FP E  KQ   ++ + N + N+VAFK QTT PK   ++P  G++ P  S    V      
Sbjct: 16  FPIELNKQGSCSLNLTNKTDNYVAFKAQTTKPKMYCVKPSVGVVLPRSSCEVLVVMQALK 75

Query: 145 LDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPA 204
               D+    K   + K+V      D +   E+F ++  H   E I +++++ P      
Sbjct: 76  EAPADRQCKDKLLFQCKVVEPGTM-DKEVTSEMFSKEAGHRVEETIFKIIYVAPPQPQSP 135

Query: 205 LEKVFRQLAEAEAAIETRQKPAE 228
           +++     +   A++  +   +E
Sbjct: 136 VQEGLEDGSSPSASVSDKGNASE 157

BLAST of HG10000962 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.4e-06
Identity = 48/185 (25.95%), Postives = 85/185 (45.95%), Query Frame = 0

Query: 81  NKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFK 140
           ++L F FE  KQ    +++ N ++N+VAFK +TT+PK  ++RP  G++ P +S I  V  
Sbjct: 15  DELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDSCIIRVTL 74

Query: 141 FVEPLDNNDKHVDHKRRVKFKIVSLKV--KGDMDYVP-ELFDEQKDHVAVEQILQVVFLD 200
             +     +   D + + KF + S  V    D+D +P + F +       E  L+V ++ 
Sbjct: 75  QAQ----REYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVSYIT 134

Query: 201 PDHSNPALEK-VFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQV 260
           P  +  + E        ++   I T Q+  EE D  +     +  E +  R R   +   
Sbjct: 135 PSTTQRSSESGATNGDGQSSETISTIQRLKEERDAAVKQTQQLQHELETVRRRRNQRNSG 194

Query: 261 GGVGL 262
            G+ L
Sbjct: 195 NGLSL 195

BLAST of HG10000962 vs. ExPASy TrEMBL
Match: A0A0A0KE43 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 6.6e-132
Identity = 242/266 (90.98%), Postives = 248/266 (93.23%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP----SSASSSSLIHRVHLQSHSHKPAEHSSS 60
           MAVA+EKSG D KVW+LCSIPFWQNSNAP    SS+SSSS+IHRVHLQS+ HKPAEHSSS
Sbjct: 1   MAVADEKSGPDGKVWSLCSIPFWQNSNAPSSSSSSSSSSSVIHRVHLQSNGHKPAEHSSS 60

Query: 61  HPGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAP 120
           HPGVVSAIAKSL PTKRRLRLDPSN LYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAP
Sbjct: 61  HPGVVSAIAKSLCPTKRRLRLDPSNNLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAP 120

Query: 121 KSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPE 180
           KSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK D+DYVPE
Sbjct: 121 KSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDLDYVPE 180

Query: 181 LFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGG 240
           LFDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGG
Sbjct: 181 LFDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGG 240

Query: 241 GLVLDEWKERRERYLAKQQVGGVGLV 263
           GLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 GLVLDEWKERRERYLAKQQVGGVDLV 266

BLAST of HG10000962 vs. ExPASy TrEMBL
Match: A0A5D3BT76 (Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold388G00900 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 9.5e-131
Identity = 241/265 (90.94%), Postives = 246/265 (92.83%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120

Query: 121 SCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 180
           SCYMRPPGGILAPDES IATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK DMDYVPEL
Sbjct: 121 SCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPEL 180

Query: 181 FDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGG 240
           FDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGGG
Sbjct: 181 FDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGGG 240

Query: 241 LVLDEWKERRERYLAKQQVGGVGLV 263
           LVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 LVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of HG10000962 vs. ExPASy TrEMBL
Match: A0A1S3BJS8 (vesicle-associated protein 4-2-like OS=Cucumis melo OX=3656 GN=LOC103490476 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 9.5e-131
Identity = 241/265 (90.94%), Postives = 246/265 (92.83%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120

Query: 121 SCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 180
           SCYMRPPGGILAPDES IATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK DMDYVPEL
Sbjct: 121 SCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPEL 180

Query: 181 FDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGG 240
           FDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGGG
Sbjct: 181 FDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGGG 240

Query: 241 LVLDEWKERRERYLAKQQVGGVGLV 263
           LVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 LVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of HG10000962 vs. ExPASy TrEMBL
Match: A0A5A7U105 (Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001980 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.7e-130
Identity = 240/265 (90.57%), Postives = 245/265 (92.45%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPK 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSK HVAFKFQTTAPK
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKTHVAFKFQTTAPK 120

Query: 121 SCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 180
           SCYMRPPGGILAPDES IATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVK DMDYVPEL
Sbjct: 121 SCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPEL 180

Query: 181 FDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGG 240
           FDEQKD VAVEQILQ VFLDPDHSNPALEKV RQLAEAEAA E R KPAEE DPKIVGGG
Sbjct: 181 FDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGGG 240

Query: 241 LVLDEWKERRERYLAKQQVGGVGLV 263
           LVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 LVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of HG10000962 vs. ExPASy TrEMBL
Match: A0A6J1I192 (vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.9e-119
Identity = 222/264 (84.09%), Postives = 241/264 (91.29%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSN-APSSASSSSLIHRVHLQSHSHKPAEHSSSHP- 60
           MAVA++KS  D KVW+LCSIPFWQN N + SS SSS+ IHRV+ QS+ HKPAE+S S+P 
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKS 120
           G VS IAKSLLPT+RRLRLDPSNKLYFP+EPGKQVKSAIEI+NTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELF 180
           CYMRPPGGILAP ESIIATVFKFVEPL+NN+K VDHKRRVKFKIVSLKVKG+MDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDHKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGL 240
           DEQ+DHVAVEQILQVVFLDP HSNPALEK+FRQLA AEAA E RQKPAEE DPK+VG GL
Sbjct: 181 DEQRDHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VLDEWKERRERYLAKQQVGGVGLV 263
           V+DEWKERRERYLAKQQVGGV LV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of HG10000962 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 4.0e-89
Identity = 170/272 (62.50%), Postives = 203/272 (74.63%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFK 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP+EPGKQV+SAI+IKNTSK+HVAFK
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFK 146

Query: 128 FQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGD 187
           FQTTAPKSC+MRPPG ILAP E+IIATVFKFVEP +NN+K +D + RVKFKI+SLKVKG 
Sbjct: 147 FQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGP 206

Query: 188 MDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEAD 247
           MDYVPELFDEQKD V+ EQIL+V+FLDP+ SNPALEK+ RQLAEA+AA+E R+KP EE  
Sbjct: 207 MDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETG 266

Query: 248 PKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 263
           PK++G GLV+DEWKERRERYLA+QQ  G   V
Sbjct: 267 PKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of HG10000962 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.0e-85
Identity = 170/291 (58.42%), Postives = 203/291 (69.76%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPF-------------------EPGK 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP+                   EPGK
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPWFVSEDLRSIDTSLKFLSFNEPGK 146

Query: 128 QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKH 187
           QV+SAI+IKNTSK+HVAFKFQTTAPKSC+MRPPG ILAP E+IIATVFKFVEP +NN+K 
Sbjct: 147 QVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPENNEKP 206

Query: 188 VDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQ 247
           +D + RVKFKI+SLKVKG MDYVPELFDEQKD V+ EQIL+V+FLDP+ SNPALEK+ RQ
Sbjct: 207 MDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEKLKRQ 266

Query: 248 LAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 263
           LAEA+AA+E R+KP EE  PK++G GLV+DEWKERRERYLA+QQ  G   V
Sbjct: 267 LAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADSV 314

BLAST of HG10000962 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 295.8 bits (756), Expect = 3.4e-80
Identity = 151/251 (60.16%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 12  EKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHS--SSHPGVVSAIAKSLL 71
           + ++ LC  PFWQ  +  SS+S+ +  ++ +   H ++  + S  S  P  +S++A+SLL
Sbjct: 15  KNLFRLC--PFWQRRSTTSSSSTQN-PNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 74

Query: 72  PTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILA 131
           P +RRLRLDPS+ LYFP+EPGKQV+SAI++KNTSK+H AFKFQTTAPKSCYMRPPGG+LA
Sbjct: 75  PARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLA 134

Query: 132 PDESIIATVFKFVEPLDNNDKH-VDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVE 191
           P ES+ ATVFKFVE  +NN+K  ++ K +VKFKI+SLKVK  ++YVPELFDEQKD VAVE
Sbjct: 135 PGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVE 194

Query: 192 QILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRE 251
           Q+L+V+F+D D  + ALEK+ RQL EAEAA+E R+KP  E  P++VG GLV+DEWKERRE
Sbjct: 195 QVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERRE 254

Query: 252 RYLAKQQVGGV 260
           +YLA+QQV  V
Sbjct: 255 KYLARQQVESV 262

BLAST of HG10000962 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 289.7 bits (740), Expect = 2.4e-78
Identity = 161/285 (56.49%), Postives = 204/285 (71.58%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFW-QNSNAPSSASSSSL------IHRVHL-QSHSHKPAE 60
           MA+  +KS  D + W    +PF   NS APS++SSSS+      +   HL Q++ H+   
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 61  HSSSHPGVV-----------------SAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKS 120
                P VV                 S++A+SLLPTKRRL+LDPS KLYFP+EPGKQV+S
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQVRS 120

Query: 121 AIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHK 180
           AI+IKNTSK+HVAFKFQTT PKSC+MRP G ILAP E IIATVFKFVEP +NN+K ++ K
Sbjct: 121 AIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPMEQK 180

Query: 181 RRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEA 240
             VKFKI+SLK+K   DY+PELF+EQKDHV+ EQ+++VVFLDP++ N  +EK+  QLAEA
Sbjct: 181 SGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQLAEA 240

Query: 241 EAAIETRQKPAEE-ADPKIVGGGLVLDEWKERRERYLAKQQVGGV 260
           +AA E R+K +E    PK +G GLV+DEWK+RRERYLA+QQ GGV
Sbjct: 241 DAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGV 284

BLAST of HG10000962 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-53
Identity = 111/187 (59.36%), Postives = 132/187 (70.59%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFK 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP+EPGKQV+SAI+IKNTSK+HVAFK
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPYEPGKQVRSAIKIKNTSKSHVAFK 146

Query: 128 FQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGD 178
           FQTTAPKSC+MRPPG ILAP E+IIATVFKFVEP +NN+K +D + RVKFKI+SLKVKG 
Sbjct: 147 FQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGP 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011656923.22.3e-13190.30vesicle-associated protein 4-2 [Cucumis sativus] >KGN46696.2 hypothetical protei... [more]
XP_038900695.18.8e-13194.47vesicle-associated protein 4-2-like [Benincasa hispida][more]
XP_008448221.12.0e-13090.94PREDICTED: vesicle-associated protein 4-2-like [Cucumis melo] >TYK02188.1 vesicl... [more]
KAA0047916.19.7e-13090.57vesicle-associated protein 4-2-like [Cucumis melo var. makuwa][more]
XP_022970681.15.9e-11984.09vesicle-associated protein 4-2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VYN25.6e-8862.50Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q1ECE04.8e-7960.16Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q8LPQ73.4e-7756.49Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q9SYC92.4e-0623.78Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
Q9LVU15.4e-0625.95Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KE436.6e-13290.98MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV... [more]
A0A5D3BT769.5e-13190.94Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BJS89.5e-13190.94vesicle-associated protein 4-2-like OS=Cucumis melo OX=3656 GN=LOC103490476 PE=4... [more]
A0A5A7U1054.7e-13090.57Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A6J1I1922.9e-11984.09vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 ... [more]
Match NameE-valueIdentityDescription
AT4G21450.14.0e-8962.50PapD-like superfamily protein [more]
AT4G21450.32.0e-8558.42PapD-like superfamily protein [more]
AT5G54110.13.4e-8060.16membrane-associated mannitol-induced [more]
AT4G05060.12.4e-7856.49PapD-like superfamily protein [more]
AT4G21450.21.2e-5359.36PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 69..199
e-value: 7.0E-29
score: 102.3
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 75..185
e-value: 2.2E-29
score: 101.5
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 74..196
score: 22.121742
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 35..59
NoneNo IPR availablePANTHERPTHR10809:SF101VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 35..259
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 35..259
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 64..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000962.1HG10000962.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane