HG10000960 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000960
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptioncoumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like
LocationChr09: 12494232 .. 12495671 (+)
RNA-Seq ExpressionHG10000960
SyntenyHG10000960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAGTCCTCTTGTTTTTCAGTAAAGATTATAGAGAAGTGCTTGGTTTGTCCACCGCCGGGCTCCGTTCCCACAACCCATCTCCCGCTCAGCTACTTCGACATAATTTGGCTACGACATGCACCCAAATGCCGCCTTTTCTTCTACAAATTTCCACACTCCACCGCTCATTTTACCAACACCATCCTCCCATCTCTCAAACAATCTCTTTCCCTTACTCTCCAACACTTCTTCCCTCTTTCTGCCAATCTCATCATCCCCCCCCAACCTCAGAAACCCCATCTCCTCTATACCCCTCAAGACTCTATTCATCTAGCGATAGCCGAATCTGATTCTCCCCATTTCCATTCCCTTCTACCTGATCATCCAAAAGATGCCAAAGATGTGTACCAATTATTCCAAGATTGGCCTGAGTTTCGCTTCTTGTCAGATGGTACTCAAGTCATTCCTCTCTTAACTCTACAAATCACCATCTTCCCCTGTCAAGCCCTTTCCATAGGTGCATCATTTCTCCACGTGGGCGTCGACGGACCTGCACTCCATCATTTCATCAAATCATGGGCGTCCATTTTCGCAGGAAAAGAGCTTGATGCCCCACTACCGTTTCACGATCGAACTATTGTCCAAGATCCACATGGACTTGAATCCACTTTTCTTCGTCTTCAGTGGAGCATTGCTGGACCCATCAATTTTTATACTGAGCCCATCAATCAGCGTGTTGGTGACAAGGTTCGAGCAACTCTCTCCTTGTCCAAAGACCAAATTGAGAGACTAAAAAATTGGGGGAATTCACAAATGACCAAATTACCCCAACCCTTAGAAATGGAATCTCCATATTTGTCGACTTTTGTAGTGACATGCTCTCTGGTTTGGGTTTGTTATGTCGCGTCACGTGAAGGAGGAGAAAGCGAATTAGTTTGCAAAGACGAATTGTACTACTTTTCATTTGTAGCGAATTGTCGTAATGGACGACTTGGGTATCCAATACCTTCGGGGTATTTTGGGAATTGTCTGGCATTCTGCATTGCAGCAGTAAAGAAGAGTGAGCTTTTGGGGGAAAATGGGATGGTTATGGGAATGAAAGCAATTGGGAAAAGAGTGAGGGAATTGGAAAAAGAGGCAGTGAAAGACATAGTGGAGGAGATGTCCATATGGATGAAGTGTGTAGAAACTGAAAGTATGGTTGCAGTAGTTGGGTCGCCGAGGTTGGGTGCGTATGATACGGATTTTGGGTGGGGGAGACCCATAAAGACTGAGGTGGTTCAAATTGATAATGGGAGATTATTTTCTTTGGGAGAATCAAGAGATGAAAAAGGTGGAATTGAAGTTGGGTTGGCTTTGAGTTCGGCTTATATGACTCGCTTTGTTGCCGTGTGGGAACAAAATCTAAAGCTTTTCGATTGCCTTTCAGAGACGCATTATATGAGATTTTTATAG

mRNA sequence

ATGGCCGAGTCCTCTTGTTTTTCAGTAAAGATTATAGAGAAGTGCTTGGTTTGTCCACCGCCGGGCTCCGTTCCCACAACCCATCTCCCGCTCAGCTACTTCGACATAATTTGGCTACGACATGCACCCAAATGCCGCCTTTTCTTCTACAAATTTCCACACTCCACCGCTCATTTTACCAACACCATCCTCCCATCTCTCAAACAATCTCTTTCCCTTACTCTCCAACACTTCTTCCCTCTTTCTGCCAATCTCATCATCCCCCCCCAACCTCAGAAACCCCATCTCCTCTATACCCCTCAAGACTCTATTCATCTAGCGATAGCCGAATCTGATTCTCCCCATTTCCATTCCCTTCTACCTGATCATCCAAAAGATGCCAAAGATGTGTACCAATTATTCCAAGATTGGCCTGAGTTTCGCTTCTTGTCAGATGGTACTCAAGTCATTCCTCTCTTAACTCTACAAATCACCATCTTCCCCTGTCAAGCCCTTTCCATAGGTGCATCATTTCTCCACGTGGGCGTCGACGGACCTGCACTCCATCATTTCATCAAATCATGGGCGTCCATTTTCGCAGGAAAAGAGCTTGATGCCCCACTACCGTTTCACGATCGAACTATTGTCCAAGATCCACATGGACTTGAATCCACTTTTCTTCGTCTTCAGTGGAGCATTGCTGGACCCATCAATTTTTATACTGAGCCCATCAATCAGCGTGTTGGTGACAAGGTTCGAGCAACTCTCTCCTTGTCCAAAGACCAAATTGAGAGACTAAAAAATTGGGGGAATTCACAAATGACCAAATTACCCCAACCCTTAGAAATGGAATCTCCATATTTGTCGACTTTTGTAGTGACATGCTCTCTGGTTTGGGTTTGTTATGTCGCGTCACGTGAAGGAGGAGAAAGCGAATTAGTTTGCAAAGACGAATTGTACTACTTTTCATTTGTAGCGAATTGTCGTAATGGACGACTTGGGTATCCAATACCTTCGGGGTATTTTGGGAATTGTCTGGCATTCTGCATTGCAGCAGTAAAGAAGAGTGAGCTTTTGGGGGAAAATGGGATGGTTATGGGAATGAAAGCAATTGGGAAAAGAGTGAGGGAATTGGAAAAAGAGGCAGTGAAAGACATAGTGGAGGAGATGTCCATATGGATGAAGTGTGTAGAAACTGAAAGTATGGTTGCAGTAGTTGGGTCGCCGAGGTTGGGTGCGTATGATACGGATTTTGGGTGGGGGAGACCCATAAAGACTGAGGTGGTTCAAATTGATAATGGGAGATTATTTTCTTTGGGAGAATCAAGAGATGAAAAAGGTGGAATTGAAGTTGGGTTGGCTTTGAGTTCGGCTTATATGACTCGCTTTGTTGCCGTGTGGGAACAAAATCTAAAGCTTTTCGATTGCCTTTCAGAGACGCATTATATGAGATTTTTATAG

Coding sequence (CDS)

ATGGCCGAGTCCTCTTGTTTTTCAGTAAAGATTATAGAGAAGTGCTTGGTTTGTCCACCGCCGGGCTCCGTTCCCACAACCCATCTCCCGCTCAGCTACTTCGACATAATTTGGCTACGACATGCACCCAAATGCCGCCTTTTCTTCTACAAATTTCCACACTCCACCGCTCATTTTACCAACACCATCCTCCCATCTCTCAAACAATCTCTTTCCCTTACTCTCCAACACTTCTTCCCTCTTTCTGCCAATCTCATCATCCCCCCCCAACCTCAGAAACCCCATCTCCTCTATACCCCTCAAGACTCTATTCATCTAGCGATAGCCGAATCTGATTCTCCCCATTTCCATTCCCTTCTACCTGATCATCCAAAAGATGCCAAAGATGTGTACCAATTATTCCAAGATTGGCCTGAGTTTCGCTTCTTGTCAGATGGTACTCAAGTCATTCCTCTCTTAACTCTACAAATCACCATCTTCCCCTGTCAAGCCCTTTCCATAGGTGCATCATTTCTCCACGTGGGCGTCGACGGACCTGCACTCCATCATTTCATCAAATCATGGGCGTCCATTTTCGCAGGAAAAGAGCTTGATGCCCCACTACCGTTTCACGATCGAACTATTGTCCAAGATCCACATGGACTTGAATCCACTTTTCTTCGTCTTCAGTGGAGCATTGCTGGACCCATCAATTTTTATACTGAGCCCATCAATCAGCGTGTTGGTGACAAGGTTCGAGCAACTCTCTCCTTGTCCAAAGACCAAATTGAGAGACTAAAAAATTGGGGGAATTCACAAATGACCAAATTACCCCAACCCTTAGAAATGGAATCTCCATATTTGTCGACTTTTGTAGTGACATGCTCTCTGGTTTGGGTTTGTTATGTCGCGTCACGTGAAGGAGGAGAAAGCGAATTAGTTTGCAAAGACGAATTGTACTACTTTTCATTTGTAGCGAATTGTCGTAATGGACGACTTGGGTATCCAATACCTTCGGGGTATTTTGGGAATTGTCTGGCATTCTGCATTGCAGCAGTAAAGAAGAGTGAGCTTTTGGGGGAAAATGGGATGGTTATGGGAATGAAAGCAATTGGGAAAAGAGTGAGGGAATTGGAAAAAGAGGCAGTGAAAGACATAGTGGAGGAGATGTCCATATGGATGAAGTGTGTAGAAACTGAAAGTATGGTTGCAGTAGTTGGGTCGCCGAGGTTGGGTGCGTATGATACGGATTTTGGGTGGGGGAGACCCATAAAGACTGAGGTGGTTCAAATTGATAATGGGAGATTATTTTCTTTGGGAGAATCAAGAGATGAAAAAGGTGGAATTGAAGTTGGGTTGGCTTTGAGTTCGGCTTATATGACTCGCTTTGTTGCCGTGTGGGAACAAAATCTAAAGCTTTTCGATTGCCTTTCAGAGACGCATTATATGAGATTTTTATAG

Protein sequence

MAESSCFSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWASIFAGKELDAPLPFHDRTIVQDPHGLESTFLRLQWSIAGPINFYTEPINQRVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASREGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMGMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLFDCLSETHYMRFL
Homology
BLAST of HG10000960 vs. NCBI nr
Match: XP_023512859.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650.2 bits (1676), Expect = 1.4e-182
Identity = 318/469 (67.80%), Postives = 368/469 (78.46%), Query Frame = 0

Query: 1   MAESSCFSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFT 60
           MA+SSCFSVK+I+KCLV PPPGSVP T LPL++FD+I LR+AP  RLFFY+FPHSTAHFT
Sbjct: 1   MADSSCFSVKVIDKCLVSPPPGSVPATLLPLTFFDLICLRYAPTRRLFFYEFPHSTAHFT 60

Query: 61  NTILPSLKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLL 120
           + ILPSLK+SLSL+LQHFFP +ANLI+PP P KPH LYTP DS+ LAIAESD  HFH+L+
Sbjct: 61  HAILPSLKRSLSLSLQHFFPFAANLILPPHPHKPHFLYTPHDSVQLAIAESDPSHFHALI 120

Query: 121 PDHPKDAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPA 180
           PDHPKDA+DVY LFQDWP    LSDGTQVIPL+ LQITIFP  ALS+  SFLHV  DGPA
Sbjct: 121 PDHPKDARDVYPLFQDWPMSCLLSDGTQVIPLMALQITIFPYHALSLCVSFLHVAADGPA 180

Query: 181 LHHFIKSWASIFAGKELDAPLPFHDRTIVQDPHGLESTFLRLQWSIAGPINFYTEPINQR 240
           LHHFI+SWASI AG  L+ PLP HDRT+VQDP GLES +LR     + PINF +EPI+QR
Sbjct: 181 LHHFIRSWASISAGNGLNGPLPSHDRTVVQDPEGLESAYLRHH---SEPINFDSEPISQR 240

Query: 241 VGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASRE 300
           + DKVRAT SLSK QIERLKNW NSQ+TK        S   S+FVVT S+VWV ++ SRE
Sbjct: 241 LSDKVRATFSLSKPQIERLKNWANSQITK--------STQFSSFVVTSSVVWVSFIMSRE 300

Query: 301 GGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMG 360
                   +DE   F+F ANCRN RLGY IP  YFGNC AFC+ AV+KSELLGENG+V+ 
Sbjct: 301 LISGGSGAEDEACCFAFAANCRNERLGYRIPVEYFGNCFAFCLTAVEKSELLGENGLVVA 360

Query: 361 MKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTE 420
           MKAI KRV E+ KE +KD++ EMS W + VE  + +   GSPRLGAYD +FGWGRPIKTE
Sbjct: 361 MKAIEKRVGEVNKETLKDVMAEMSAWKEFVERGAALCAAGSPRLGAYDANFGWGRPIKTE 420

Query: 421 VVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLFDC 470
           VVQ+D GRLFS+GESR+E GGIEVGL L SA+M  FVAVWEQNLKL  C
Sbjct: 421 VVQVDRGRLFSIGESRNENGGIEVGLGLGSAHMASFVAVWEQNLKLLGC 458

BLAST of HG10000960 vs. NCBI nr
Match: XP_022140618.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Momordica charantia])

HSP 1 Score: 500.0 bits (1286), Expect = 2.3e-137
Identity = 245/385 (63.64%), Postives = 292/385 (75.84%), Query Frame = 0

Query: 90  QPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAKDVYQLFQDWPEFRFLSDG-TQ 149
           QPQKPH+L +P+DS+ L+IAESDS HFH+L+ DH KDA DVYQL QDWP FR  SDG TQ
Sbjct: 14  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 73

Query: 150 VIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWASIFAGKELDAPLPFHDRTI 209
            IPLLTL++TIFP Q LSIG SFLHV VDGPA HHF+KSWASI  G++ D P P HDRT+
Sbjct: 74  AIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 133

Query: 210 VQDPHGLESTFLRLQWSIAGPINFYTEPINQRVGDKVRATLSLSKDQIERLKNWGNSQMT 269
           V+DP+GLEST L+LQWS+AGPINF +EPI++R+ D VRAT  LSK QIE LK+W  +   
Sbjct: 134 VRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARA--- 193

Query: 270 KLPQPLEMESPYLSTFVVTCSLVWVCYVASREG----GESELVCKDELYYFSFVANCRNG 329
                   ESP+ S+FV  CSLVWVC++ SREG    G+   VC      F+F A+CR  
Sbjct: 194 --------ESPHFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVC-----CFAFAADCRK- 253

Query: 330 RLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMGMKAIGKRVRELEKEAVKDIVEEMS 389
           RLGYP+P+GYFGNCLAFC A V+K ELLG +G+++GMKA GKRV E E E +K+  E  S
Sbjct: 254 RLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWAS 313

Query: 390 IWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEV 449
           +W K  E   + AV GSPRLGAYD DFGWGRPIKT+V+ ID+G LFSLGESR+E GGIEV
Sbjct: 314 VWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEV 373

Query: 450 GLALSSAYMTRFVAVWEQNLKLFDC 470
           GLALS+A+M RFVAVWEQ L LF C
Sbjct: 374 GLALSTAHMARFVAVWEQTLNLFSC 381

BLAST of HG10000960 vs. NCBI nr
Match: XP_023533558.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.8 bits (1161), Expect = 7.2e-123
Identity = 229/466 (49.14%), Postives = 306/466 (65.67%), Query Frame = 0

Query: 7   FSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPS 66
           ++ K++    + PP GSVPTT LPLS+ D IW+   P  RLFF++FPHST HF +++ P+
Sbjct: 44  YTAKLLNLYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPT 103

Query: 67  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 126
           LK+SLSL+LQHFFPL+ NLI+PPQP KPH+LYTP DS+ L +AES    F +L+ D+P+D
Sbjct: 104 LKRSLSLSLQHFFPLAGNLILPPQPHKPHILYTPHDSVPLTVAESTGGDFTTLIADYPRD 163

Query: 127 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 186
           AK  Y    D PE R L DG++ IP   +QITIFP Q L I  +F HV  DG A+HHFIK
Sbjct: 164 AKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAIHHFIK 223

Query: 187 SWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYTEPINQ 246
           SWASI          + PLP H+R  ++D  GLES FL+    W S+  P+  Y   I+Q
Sbjct: 224 SWASISRNGGTSDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVP-YKPIIDQ 283

Query: 247 RVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASR 306
              D+VR TL L++  IE+LK W +++        E++   LS+F+VTCSLVW C + S 
Sbjct: 284 APVDRVRTTLVLTRPMIEKLKTWVSAESN---NDEELKPLRLSSFIVTCSLVWGCLIKST 343

Query: 307 EGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVM 366
           +   S+   +DEL YF F+A+CRN R+  P+P  YFGNCL   +  +KKSEL+  N +VM
Sbjct: 344 QSEASDSEDEDELCYFGFLADCRN-RVKDPLPPTYFGNCLDVGVVELKKSELVKRNSIVM 403

Query: 367 GMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKT 426
             KAIG RV E E E +K   +  S+W    + +  V + GSP+L AYDTDFGWGR +K+
Sbjct: 404 AAKAIGNRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAMKS 463

Query: 427 EVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           EV+ +DN    SL +SRDE+GGIE+GL L +  M RF+A+WEQNLK
Sbjct: 464 EVLHVDNSESISLQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLK 504

BLAST of HG10000960 vs. NCBI nr
Match: XP_022971000.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 447.6 bits (1150), Expect = 1.4e-121
Identity = 227/466 (48.71%), Postives = 305/466 (65.45%), Query Frame = 0

Query: 7   FSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPS 66
           ++ K++    + PP GSVPTT LP+S+ D IW+   P  RLFF++FPHST HF +++ P+
Sbjct: 6   YTAKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPT 65

Query: 67  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 126
           LK SLSL+LQHFFPL+ NLI+PPQPQKPH+LYTP DS+ L + ES    F++L+ D+P+D
Sbjct: 66  LKSSLSLSLQHFFPLAGNLILPPQPQKPHILYTPHDSVPLTVTESTGGDFNTLIADYPRD 125

Query: 127 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 186
           AK  Y    D PE R L DG++ IP   +QITIFP Q L I  +F HV  DG A+HHFIK
Sbjct: 126 AKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHVVADGIAIHHFIK 185

Query: 187 SWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYTEPINQ 246
           SWASI          + PLP H+R  ++D  GLES FL+    W S+  P+  Y   I+Q
Sbjct: 186 SWASISRNGGTSDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVP-YKPIIDQ 245

Query: 247 RVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASR 306
              D+VR TL L++  IE+LK W +++        E++   LS+F+VTCSLVW C + S 
Sbjct: 246 APVDRVRTTLVLTRPMIEKLKTWVSAESN---NDEELKPLRLSSFIVTCSLVWGCLIKST 305

Query: 307 EGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVM 366
           +   S+   +DEL +F F+A+CRN RL  P+P  YFGNCL   +  +KKSEL+  N  VM
Sbjct: 306 QSEVSDSEDEDELCHFGFLADCRN-RLKDPLPPTYFGNCLDVGVVELKKSELVKRNSTVM 365

Query: 367 GMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKT 426
             KAIG RV E E E +K   +  S+W    + +  V + GSP+L AYDTDFGWGR +K+
Sbjct: 366 AAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRAYDTDFGWGRAMKS 425

Query: 427 EVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           EV+ +D+    SL +SRDE+GGIE+GL L +  M RF+A+WEQNLK
Sbjct: 426 EVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNLK 466

BLAST of HG10000960 vs. NCBI nr
Match: XP_022947400.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 446.0 bits (1146), Expect = 4.0e-121
Identity = 226/466 (48.50%), Postives = 306/466 (65.67%), Query Frame = 0

Query: 7   FSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPS 66
           ++ K++    + PP GSVPTT LPLS+ D IW+   P  RLFF++FPHST HF +++ P+
Sbjct: 44  YTAKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPT 103

Query: 67  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 126
           LK+SLSL+LQHFFPL+ NLI+PP P KPH+LYTP DS+ L + ES    F++L+ D+P+D
Sbjct: 104 LKRSLSLSLQHFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIADYPRD 163

Query: 127 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 186
           AK  Y    D PE   L DG++ IP   +QITIFP Q L I  +F HV  DG A+HHFIK
Sbjct: 164 AKHFYPFVPDMPESCLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAIHHFIK 223

Query: 187 SWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYTEPINQ 246
           SWASI     A    + PLP H+R  ++D  GLES FL+    W S+  P+  Y   I+Q
Sbjct: 224 SWASISRNGGASDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVP-YKPIIDQ 283

Query: 247 RVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASR 306
              D+VR TL L++  IE+LK W +++        E +   LS+F+VTCSLVW C + S 
Sbjct: 284 APVDRVRTTLVLTRPMIEKLKTWVSAESN---NDEESKPLRLSSFIVTCSLVWGCLIKST 343

Query: 307 EGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVM 366
           +   S+ V +D+L YF F+A+CRN R+  P+P  YFGNCL   +  +KKSEL+  N +VM
Sbjct: 344 QSEASDSVDEDKLCYFGFLADCRN-RVKDPLPPTYFGNCLDVGVVELKKSELVKRNSIVM 403

Query: 367 GMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKT 426
             KAIGKRV E E E +K   +  S+W    + +  V + GSP+L AYDTDFGWGR +K+
Sbjct: 404 AAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAMKS 463

Query: 427 EVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           EV+ +D+    S+ +SRDE+GGIE+GL L +  M RF+A+WEQNLK
Sbjct: 464 EVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLK 504

BLAST of HG10000960 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 5.1e-79
Identity = 180/479 (37.58%), Postives = 262/479 (54.70%), Query Frame = 0

Query: 10  KIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQ 69
           KIIE C + PP G+VP+T LPL++FD  WL       LFF+ + +ST  F    +P+LK 
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 70  SLSLTLQHFFPLSANLIIPPQPQKPHLLYTP-QDSIHLAIAESDSPHFHSLLPDHPKDAK 129
           SLS+TLQHFFP +  LIIPP+P  P+L Y   QDS+   +AES    F  L  D PKD  
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 130 DVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSW 189
            ++ +    P      +G Q+ P++ +Q+TIFP   + IG S  HV  DG    HF+K W
Sbjct: 129 VLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYW 188

Query: 190 ASI--FAGKE----LDAPLPFHD-RTIVQDPHGLESTFLRLQWS-IAGPINFYTEPINQR 249
            S+   +GK+    L   LP H  R +++DP  + +  L   WS  +   + +  P N  
Sbjct: 189 MSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQNSAKHSSHVTPENM- 248

Query: 250 VGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYV---- 309
               VRAT +LS+ QI+ LK+W   Q        E +SP +STFVVT + +WV  +    
Sbjct: 249 ----VRATFTLSRKQIDNLKSWVTEQS-------ENQSP-VSTFVVTLAFIWVSLIKTLV 308

Query: 310 --ASREGGESELVCKDELYYFSFVANCRNGRLGY--PIPSGYFGNCLAFCIAAVKKSELL 369
             +  +  E +   KDE+++     +CRN RL Y  PIP  YFGNC+A  I +VKK +LL
Sbjct: 309 QDSETKANEED---KDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLL 368

Query: 370 GENGMVMGMKAIGKRVRELEKEAVKDIVEEMSIW----MKCVETESMVAVVGSPRLGAYD 429
           GE  ++    AI  R++++      D+++    W     K V +    ++ G+P+LG YD
Sbjct: 369 GEKCVLAASDAITARIKDM---LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYD 428

Query: 430 TDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLF 468
            DFG G+P K E+V I+ G   +  ESRD   G+E+G+AL    M  F ++ +Q +K F
Sbjct: 429 MDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKF 467

BLAST of HG10000960 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.3e-78
Identity = 178/472 (37.71%), Postives = 260/472 (55.08%), Query Frame = 0

Query: 11  IIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQS 70
           IIE C + PP G+V +T LPL++FD  WL       LFF+ + +ST  F    +P+LK S
Sbjct: 10  IIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 71  LSLTLQHFFPLSANLIIPPQPQKPHLLYTP-QDSIHLAIAESDSPHFHSLLPDHPKDAKD 130
           LS+TLQHFFP +  LIIPP+P  P+L Y   +DS+   +AES    F  L  D PKD   
Sbjct: 70  LSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLKSDSPKDISV 129

Query: 131 VYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWA 190
           ++ +    P      +G Q+ P++ +Q+TIFP   + IG S  HV  DG    HF+K W 
Sbjct: 130 LHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYWM 189

Query: 191 SI--FAGKE----LDAPLPFHD-RTIVQDPHGLESTFLRLQWS-IAGPINFYTEPINQRV 250
           S+   +GK+    L   LP H  R I++DP  + +  L   WS  +   + +  P N   
Sbjct: 190 SLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQNSAKHSSHVTPENM-- 249

Query: 251 GDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASREG 310
              VRAT +LS+ QI+ LK+W   Q        E +SP +STFVVT + +WV  + +   
Sbjct: 250 ---VRATFTLSRKQIDNLKSWVTEQS-------ENQSP-VSTFVVTLAFIWVSLIKTLV- 309

Query: 311 GESELVCKDELYYFSFVANCRNGRLGY--PIPSGYFGNCLAFCIAAVKKSELLGENGMVM 370
            +SE   KDE+++     +CRN RL Y  PIP  YFGNC+A  I +VKK +LLGE  ++ 
Sbjct: 310 QDSETEAKDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMA 369

Query: 371 GMKAIGKRVRELEKEAVKDIVEEMSIW----MKCVETESMVAVVGSPRLGAYDTDFGWGR 430
              AI  R++++      D+++    W     K V +    ++ G+P+LG YD DFG G+
Sbjct: 370 ASDAITARIKDM---LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGK 429

Query: 431 PIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLF 468
           P K E+V I+ G   +  ESRD   G+E+G+AL    M  F ++ ++ +K F
Sbjct: 430 PCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKF 463

BLAST of HG10000960 vs. ExPASy Swiss-Prot
Match: Q9ZWR8 (Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.3e-71
Identity = 167/470 (35.53%), Postives = 257/470 (54.68%), Query Frame = 0

Query: 9   VKIIEKCLVCPPPGSVPT-THLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSL 68
           VK++EKC V PP  +      LP+++FDI WL       L FY FP+   HF +T++P+L
Sbjct: 7   VKVLEKCQVTPPSDTTDVELSLPVTFFDIPWLHLNKMQSLLFYDFPYPRTHFLDTVIPNL 66

Query: 69  KQSLSLTLQHFFPLSANLIIP-PQPQKPHLLYTPQ--DSIHLAIAESDSPHFHSLLPDHP 128
           K SLSLTL+H+ PLS NL++P    + P   Y+    DSI L +AESD   F  L     
Sbjct: 67  KASLSLTLKHYVPLSGNLLMPIKSGEMPKFQYSRDEGDSITLIVAESDQ-DFDYLKGHQL 126

Query: 129 KDAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHF 188
            D+ D++ LF   P         +VIPL+ +Q+T+FP + +++  +  H   D  +   F
Sbjct: 127 VDSNDLHGLFYVMPRVIRTMQDYKVIPLVAVQVTVFPNRGIAVALTAHHSIADAKSFVMF 186

Query: 189 IKSWASIFA-GKELDA----PLPFHDRTIVQDPHGLESTFLRLQWSIAGPIN-FYTEPIN 248
           I +WA I   GK+ D      LP  DR+I++D +GLE TF      +    + F ++P  
Sbjct: 187 INAWAYINKFGKDADLLSANLLPSFDRSIIKDLYGLEETFWNEMQDVLEMFSRFGSKPPR 246

Query: 249 QRVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVAS 308
               +KVRAT  LS  +I++LKN   +     P      +  ++TF +TC  VW C V S
Sbjct: 247 ---FNKVRATYVLSLAEIQKLKNKVLNLRGSEP------TIRVTTFTMTCGYVWTCMVKS 306

Query: 309 REGGESELVCKD--ELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENG 368
           ++   SE    D  EL YFSF A+CR G L  P P  YFGNCLA C+A     EL+G+ G
Sbjct: 307 KDDVVSEESSNDENELEYFSFTADCR-GLLTPPCPPNYFGNCLASCVAKATHKELVGDKG 366

Query: 369 MVMGMKAIGKRV-RELEKEAVKDIVEEMSIWM---KCVETESMVAVVGSPRLGAYDTDFG 428
           +++ + AIG+ + + L  E  K ++ +   W+     + ++  + + GSP+  +Y  DFG
Sbjct: 367 LLVAVAAIGEAIEKRLHNE--KGVLADAKTWLSESNGIPSKRFLGITGSPKFDSYGVDFG 426

Query: 429 WGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQ 463
           WG+P K ++  +D   L  + +SRD + G+E+G++L   +M  F  ++E+
Sbjct: 427 WGKPAKFDITSVDYAELIYVIQSRDFEKGVEIGVSLPKIHMDAFAKIFEE 463

BLAST of HG10000960 vs. ExPASy Swiss-Prot
Match: Q589Y0 (Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.6e-70
Identity = 161/471 (34.18%), Postives = 255/471 (54.14%), Query Frame = 0

Query: 11  IIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQS 70
           +IE+C V P PGS     LPL+YFD +WL      R+ FYK P S   F  TI+P+LK S
Sbjct: 4   VIEQCQVVPSPGSATELTLPLTYFDHVWLAFHRMRRILFYKLPISRPDFVQTIIPTLKDS 63

Query: 71  LSLTLQHFFPLSANLIIPPQ-PQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAKD 130
           LSLTL+++ PL+ N+  P      P L Y   +S+ +  +ESD   F+ L+  HP++ KD
Sbjct: 64  LSLTLKYYLPLAGNVACPQDWSGYPELRYVTGNSVSVIFSESDM-DFNYLIGYHPRNTKD 123

Query: 131 VYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWA 190
            Y       E +  + G Q+ P+L +Q+T+FP   +SIG +  HV  DG  +  F+++WA
Sbjct: 124 FYHFVPQLAEPKD-APGVQLAPVLAIQVTLFPNHGISIGFTNHHVAGDGATIVKFVRAWA 183

Query: 191 SI--FAGKE---LDAPLPFHDRTIVQDPHGLESTF---LRLQWSIAGPINFYTEPINQRV 250
            +  F G E    +  +PF+DR++++DP+G+  +    ++    +    +  T P     
Sbjct: 184 LLNKFGGDEQFLANEFIPFYDRSVIKDPNGVGMSIWNEMKKYKHMMKMSDVVTPP----- 243

Query: 251 GDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASREG 310
            DKVR T  +++  I +LKN   ++  KL         ++++F VTC+ VW C + S E 
Sbjct: 244 -DKVRGTFIITRHDIGKLKNLVLTRRPKL--------THVTSFTVTCAYVWTCIIKS-EA 303

Query: 311 GESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMGM 370
              E + ++ + +F   A+CR  +   P+P  YFGN L   +A  ++ +L G+ G  + +
Sbjct: 304 ATGEEIDENGMEFFGCAADCR-AQFNPPLPPSYFGNALVGYVARTRQVDLAGKEGFTIAV 363

Query: 371 KAIGKRVRELEKEAVKDIVEEMSI---WMK---CVETESMVAVVGSPRLGAYDTDFGWGR 430
           + IG+ +R+  K+      EE  +   W K    V+ +  ++V GSP+L  Y  DFGWGR
Sbjct: 364 ELIGEAIRKRMKD------EEWILSGSWFKEYDKVDAKRSLSVAGSPKLDLYAADFGWGR 423

Query: 431 PIKTEVVQIDN--GRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNL 465
           P K E V IDN  G   SL +S+D  G +E+GL+LS   M  F A++   +
Sbjct: 424 PEKLEFVSIDNDDGISMSLSKSKDSDGDLEIGLSLSKTRMNAFAAMFTHGI 450

BLAST of HG10000960 vs. ExPASy Swiss-Prot
Match: Q8GSN8 (Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase OS=Dahlia pinnata OX=101596 GN=3MAT PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.2e-65
Identity = 166/470 (35.32%), Postives = 241/470 (51.28%), Query Frame = 0

Query: 8   SVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSL 67
           ++ I+E   + PPP ++    LPL++FDI WL   P   L+FY FP+S +HFT T++P+L
Sbjct: 6   NLTILEHSRISPPPSTIGHRSLPLTFFDIAWLLFPPVHHLYFYHFPYSKSHFTETVIPNL 65

Query: 68  KQSLSLTLQHFFPLSANLIIPPQP----QKPHLLYTPQDSIHLAIAESDSPHFHSLLPDH 127
           K SLS+TLQH+FP    LI+ P P    +KP + +   DS+ L  AE+ +  F+ L  +H
Sbjct: 66  KHSLSITLQHYFPFVGKLIVYPNPHDSTRKPEIRHVEGDSVALTFAET-TLDFNDLSANH 125

Query: 128 PKDAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHH 187
           P+  ++ Y L          SD    +P+ ++Q+T FP   +SIG +  H   D      
Sbjct: 126 PRKCENFYPLVPPLGNAVKESDYV-TLPVFSVQVTYFPNSGISIGLTNHHSLSDANTRFG 185

Query: 188 FIKSWASIFAGKELDAPL------PFHDRTIVQDPHGLESTFLRLQWSIAGPINFYTEP- 247
           F+K+WAS+    E D P       P  DR +V +P   E+   RL  +  G   FY  P 
Sbjct: 186 FLKAWASVCETGE-DQPFLKNGSPPVFDRVVV-NPQLYEN---RLNQTRLG--TFYQAPS 245

Query: 248 INQRVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYV 307
           +     D+VRAT  L++  I  LK      +T+LP  LE    Y S+F VTC  +W C V
Sbjct: 246 LVGSSSDRVRATFVLARTHISGLK---KQVLTQLPM-LE----YTSSFTVTCGYIWSCIV 305

Query: 308 ASREGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENG 367
            S      E   +DEL  F     CR+ RL  P+P  YFGNC A CI  +K   L GENG
Sbjct: 306 KSLV-NMGEKKGEDELEQFIVSVGCRS-RLDPPLPENYFGNCSAPCIVTIKNGVLKGENG 365

Query: 368 MVMGMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETES-MVAVVGSPRLGAYDTDFGWGR 427
            VM  K IG+ + ++  +    I+E    W    +  +  + + G+P+L  YD DFGWG+
Sbjct: 366 FVMAAKLIGEGISKMVNKK-GGILEYADRWYDGFKIPARKMGISGTPKLNFYDIDFGWGK 425

Query: 428 PIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
            +K EVV ID     SL   ++     E+G+   S  M  F  ++   L+
Sbjct: 426 AMKYEVVSIDYSASVSLSACKESAQDFEIGVCFPSMQMEAFGKIFNDGLE 455

BLAST of HG10000960 vs. ExPASy TrEMBL
Match: A0A6J1CIE1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111011225 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 1.1e-137
Identity = 245/385 (63.64%), Postives = 292/385 (75.84%), Query Frame = 0

Query: 90  QPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAKDVYQLFQDWPEFRFLSDG-TQ 149
           QPQKPH+L +P+DS+ L+IAESDS HFH+L+ DH KDA DVYQL QDWP FR  SDG TQ
Sbjct: 14  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 73

Query: 150 VIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWASIFAGKELDAPLPFHDRTI 209
            IPLLTL++TIFP Q LSIG SFLHV VDGPA HHF+KSWASI  G++ D P P HDRT+
Sbjct: 74  AIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 133

Query: 210 VQDPHGLESTFLRLQWSIAGPINFYTEPINQRVGDKVRATLSLSKDQIERLKNWGNSQMT 269
           V+DP+GLEST L+LQWS+AGPINF +EPI++R+ D VRAT  LSK QIE LK+W  +   
Sbjct: 134 VRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARA--- 193

Query: 270 KLPQPLEMESPYLSTFVVTCSLVWVCYVASREG----GESELVCKDELYYFSFVANCRNG 329
                   ESP+ S+FV  CSLVWVC++ SREG    G+   VC      F+F A+CR  
Sbjct: 194 --------ESPHFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVC-----CFAFAADCRK- 253

Query: 330 RLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMGMKAIGKRVRELEKEAVKDIVEEMS 389
           RLGYP+P+GYFGNCLAFC A V+K ELLG +G+++GMKA GKRV E E E +K+  E  S
Sbjct: 254 RLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWAS 313

Query: 390 IWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEV 449
           +W K  E   + AV GSPRLGAYD DFGWGRPIKT+V+ ID+G LFSLGESR+E GGIEV
Sbjct: 314 VWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEV 373

Query: 450 GLALSSAYMTRFVAVWEQNLKLFDC 470
           GLALS+A+M RFVAVWEQ L LF C
Sbjct: 374 GLALSTAHMARFVAVWEQTLNLFSC 381

BLAST of HG10000960 vs. ExPASy TrEMBL
Match: A0A6J1I4H8 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469803 PE=3 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 6.6e-122
Identity = 227/466 (48.71%), Postives = 305/466 (65.45%), Query Frame = 0

Query: 7   FSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPS 66
           ++ K++    + PP GSVPTT LP+S+ D IW+   P  RLFF++FPHST HF +++ P+
Sbjct: 6   YTAKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPT 65

Query: 67  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 126
           LK SLSL+LQHFFPL+ NLI+PPQPQKPH+LYTP DS+ L + ES    F++L+ D+P+D
Sbjct: 66  LKSSLSLSLQHFFPLAGNLILPPQPQKPHILYTPHDSVPLTVTESTGGDFNTLIADYPRD 125

Query: 127 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 186
           AK  Y    D PE R L DG++ IP   +QITIFP Q L I  +F HV  DG A+HHFIK
Sbjct: 126 AKHFYPFVPDMPESRLLPDGSRAIPNFAIQITIFPNQGLCICIAFHHVVADGIAIHHFIK 185

Query: 187 SWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYTEPINQ 246
           SWASI          + PLP H+R  ++D  GLES FL+    W S+  P+  Y   I+Q
Sbjct: 186 SWASISRNGGTSDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVP-YKPIIDQ 245

Query: 247 RVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASR 306
              D+VR TL L++  IE+LK W +++        E++   LS+F+VTCSLVW C + S 
Sbjct: 246 APVDRVRTTLVLTRPMIEKLKTWVSAESN---NDEELKPLRLSSFIVTCSLVWGCLIKST 305

Query: 307 EGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVM 366
           +   S+   +DEL +F F+A+CRN RL  P+P  YFGNCL   +  +KKSEL+  N  VM
Sbjct: 306 QSEVSDSEDEDELCHFGFLADCRN-RLKDPLPPTYFGNCLDVGVVELKKSELVKRNSTVM 365

Query: 367 GMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKT 426
             KAIG RV E E E +K   +  S+W    + +  V + GSP+L AYDTDFGWGR +K+
Sbjct: 366 AAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRAYDTDFGWGRAMKS 425

Query: 427 EVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           EV+ +D+    SL +SRDE+GGIE+GL L +  M RF+A+WEQNLK
Sbjct: 426 EVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNLK 466

BLAST of HG10000960 vs. ExPASy TrEMBL
Match: A0A6J1G6S5 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451276 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.9e-121
Identity = 226/466 (48.50%), Postives = 306/466 (65.67%), Query Frame = 0

Query: 7   FSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPS 66
           ++ K++    + PP GSVPTT LPLS+ D IW+   P  RLFF++FPHST HF +++ P+
Sbjct: 44  YTAKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMDSVFPT 103

Query: 67  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 126
           LK+SLSL+LQHFFPL+ NLI+PP P KPH+LYTP DS+ L + ES    F++L+ D+P+D
Sbjct: 104 LKRSLSLSLQHFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIADYPRD 163

Query: 127 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 186
           AK  Y    D PE   L DG++ IP   +QITIFP Q L I  +F HV  DG A+HHFIK
Sbjct: 164 AKHFYPFVPDMPESCLLPDGSRAIPNFAIQITIFPNQGLCICIAFQHVVADGIAIHHFIK 223

Query: 187 SWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYTEPINQ 246
           SWASI     A    + PLP H+R  ++D  GLES FL+    W S+  P+  Y   I+Q
Sbjct: 224 SWASISRNGGASDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVP-YKPIIDQ 283

Query: 247 RVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASR 306
              D+VR TL L++  IE+LK W +++        E +   LS+F+VTCSLVW C + S 
Sbjct: 284 APVDRVRTTLVLTRPMIEKLKTWVSAESN---NDEESKPLRLSSFIVTCSLVWGCLIKST 343

Query: 307 EGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVM 366
           +   S+ V +D+L YF F+A+CRN R+  P+P  YFGNCL   +  +KKSEL+  N +VM
Sbjct: 344 QSEASDSVDEDKLCYFGFLADCRN-RVKDPLPPTYFGNCLDVGVVELKKSELVKRNSIVM 403

Query: 367 GMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKT 426
             KAIGKRV E E E +K   +  S+W    + +  V + GSP+L AYDTDFGWGR +K+
Sbjct: 404 AAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAMKS 463

Query: 427 EVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           EV+ +D+    S+ +SRDE+GGIE+GL L +  M RF+A+WEQNLK
Sbjct: 464 EVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNLK 504

BLAST of HG10000960 vs. ExPASy TrEMBL
Match: A0A1Q3B9H9 (Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08224 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 4.3e-121
Identity = 230/463 (49.68%), Postives = 299/463 (64.58%), Query Frame = 0

Query: 9   VKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLK 68
           +K++E+  + PPPGSVPTT LPL++FD+ WL   P  RLFFY+FPHST HF+ T LP LK
Sbjct: 24  IKVLEQSRISPPPGSVPTTTLPLTFFDMAWLLCQPMQRLFFYEFPHSTLHFSQTTLPILK 83

Query: 69  QSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAK 128
            SLSLTLQHFFPL+ANL+ PP P KPH+LY+  DSI   + ES +   H ++ +H +D +
Sbjct: 84  TSLSLTLQHFFPLAANLLCPPPPHKPHILYSDVDSIEFTVVES-TCDMHQVIGNHARDVR 143

Query: 129 DVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSW 188
           +++      P     SDGT+V+PLL +Q+T+FP   + IG  F HV  DG A +HF+KSW
Sbjct: 144 ELHPFVPRLPPSCVSSDGTRVLPLLAVQVTVFPFSGVCIGVEFSHVAADGRAFNHFMKSW 203

Query: 189 ASIFAGK-----ELDAPLPFHDRTIVQDPHGLESTFLRLQWSIAGPINFYTEPINQRVGD 248
           ASI          +D  LP H R  ++DP G+ES  L+  W  A        PIN  + D
Sbjct: 204 ASICRSNGNSLTYVDNWLPAHGRDQIEDPQGIESILLKDWWDWASNWEEDKSPINDVLAD 263

Query: 249 KVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASREGGE 308
           KVRATL L + QIERLK W +          E    +LSTFVVTCSL+WVC V S+E   
Sbjct: 264 KVRATLVLGRGQIERLKLWVS---LNCMNHNEQGQAHLSTFVVTCSLIWVCSVKSQESEV 323

Query: 309 SELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSELLGENGMVMGMKA 368
           S L   DEL YF FVA+C N RL + IP+ YFGNCLA C+  +K S+++GE+G+V   KA
Sbjct: 324 SNLHNNDELCYFCFVADCCN-RLDFSIPATYFGNCLAICVVTLKMSDIMGEDGIVAATKA 383

Query: 369 IGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTEVVQ 428
           IG +VRELE  A++   + +S W +  E   +V V GSPRL  Y+TDFGWG+PIK+EVV 
Sbjct: 384 IGNKVRELESGALRGADKWLSCWKEISEAGHVVTVAGSPRLRVYETDFGWGKPIKSEVVH 443

Query: 429 IDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKL 467
           ID  R   + ESRDE+GGIE+GLALS A +  F A +E+ L L
Sbjct: 444 IDMSRGIYICESRDEEGGIEIGLALSKAQINVFNAFFEEALML 481

BLAST of HG10000960 vs. ExPASy TrEMBL
Match: A0A6J1CHV4 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111011063 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 7.3e-121
Identity = 232/476 (48.74%), Postives = 307/476 (64.50%), Query Frame = 0

Query: 2   AESSCFSVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTN 61
           A+   ++ K++  C + PP GSVPTTHLPLS+ D++W+   P  RLFFY FPHST HF +
Sbjct: 3   ADGPDYTAKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIH 62

Query: 62  TILPSLKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLP 121
            I P+LK+SLSL LQHFFPL+ N I+PP P +PH+L TP DS+ L +AES    F  L+ 
Sbjct: 63  AIFPTLKRSLSLALQHFFPLAGNFILPPPPHRPHILCTPHDSVPLTVAESSGCDFDRLIA 122

Query: 122 DHPKDAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPAL 181
           DHP+ AK  Y L  D P  R   DG +V+P+  LQIT+FP Q L I  +F HV  DG A 
Sbjct: 123 DHPRPAKHFYPLVPDLPNSRASPDGARVVPVFALQITVFPNQGLCISIAFHHVVADGMAA 182

Query: 182 HHFIKSWASIF----AGKELDAPLPFHDRTIVQDPHGLESTFLR--LQW-SIAGPINFYT 241
           H F+KS ASI         LD PLP+HDR  V+DP+GLES FL+    W S+  P+    
Sbjct: 183 HDFLKSXASIHRNGGGSGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLWNPVP--D 242

Query: 242 EP-INQRVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWV 301
           +P I+    DKVRAT  ++K QIERLK W ++Q        E++   LS  +VTCSL+W 
Sbjct: 243 KPIIDLAPADKVRATFVINKTQIERLKTWISNQAAH-DDNEELKPLRLSALIVTCSLIWG 302

Query: 302 CYVASREG--GESELVCK-DELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVKKSE 361
           C + S EG   +++L  + DEL +F  +A+CRN RL  P+P  YFGNCL      +KKSE
Sbjct: 303 CLIKSTEGEFAQNDLPDELDELCFFVLLADCRN-RLKDPLPPTYFGNCLDMGAVVLKKSE 362

Query: 362 LLGENGMVMGMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGAYDTD 421
           LL  N  V+  KAIGKRV E E E +K   +  S W +C+E++ ++ + GSP+L  YDTD
Sbjct: 363 LLKSNSSVVAAKAIGKRVEEFESEPLKTADKWFSEW-RCLESKHLLTIAGSPKLRVYDTD 422

Query: 422 FGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKL 467
           FGWG+P K+EV+ +D+    SL E RDEKGGIEVGL L+  +M RF+A+WE+NL +
Sbjct: 423 FGWGKPKKSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLNV 473

BLAST of HG10000960 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-80
Identity = 180/479 (37.58%), Postives = 262/479 (54.70%), Query Frame = 0

Query: 10  KIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQ 69
           KIIE C + PP G+VP+T LPL++FD  WL       LFF+ + +ST  F    +P+LK 
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 70  SLSLTLQHFFPLSANLIIPPQPQKPHLLYTP-QDSIHLAIAESDSPHFHSLLPDHPKDAK 129
           SLS+TLQHFFP +  LIIPP+P  P+L Y   QDS+   +AES    F  L  D PKD  
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 130 DVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSW 189
            ++ +    P      +G Q+ P++ +Q+TIFP   + IG S  HV  DG    HF+K W
Sbjct: 129 VLHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYW 188

Query: 190 ASI--FAGKE----LDAPLPFHD-RTIVQDPHGLESTFLRLQWS-IAGPINFYTEPINQR 249
            S+   +GK+    L   LP H  R +++DP  + +  L   WS  +   + +  P N  
Sbjct: 189 MSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQNSAKHSSHVTPENM- 248

Query: 250 VGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYV---- 309
               VRAT +LS+ QI+ LK+W   Q        E +SP +STFVVT + +WV  +    
Sbjct: 249 ----VRATFTLSRKQIDNLKSWVTEQS-------ENQSP-VSTFVVTLAFIWVSLIKTLV 308

Query: 310 --ASREGGESELVCKDELYYFSFVANCRNGRLGY--PIPSGYFGNCLAFCIAAVKKSELL 369
             +  +  E +   KDE+++     +CRN RL Y  PIP  YFGNC+A  I +VKK +LL
Sbjct: 309 QDSETKANEED---KDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLL 368

Query: 370 GENGMVMGMKAIGKRVRELEKEAVKDIVEEMSIW----MKCVETESMVAVVGSPRLGAYD 429
           GE  ++    AI  R++++      D+++    W     K V +    ++ G+P+LG YD
Sbjct: 369 GEKCVLAASDAITARIKDM---LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYD 428

Query: 430 TDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLF 468
            DFG G+P K E+V I+ G   +  ESRD   G+E+G+AL    M  F ++ +Q +K F
Sbjct: 429 MDFGLGKPCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKF 467

BLAST of HG10000960 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.3e-79
Identity = 178/472 (37.71%), Postives = 260/472 (55.08%), Query Frame = 0

Query: 11  IIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTAHFTNTILPSLKQS 70
           IIE C + PP G+V +T LPL++FD  WL       LFF+ + +ST  F    +P+LK S
Sbjct: 10  IIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKHS 69

Query: 71  LSLTLQHFFPLSANLIIPPQPQKPHLLYTP-QDSIHLAIAESDSPHFHSLLPDHPKDAKD 130
           LS+TLQHFFP +  LIIPP+P  P+L Y   +DS+   +AES    F  L  D PKD   
Sbjct: 70  LSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLKSDSPKDISV 129

Query: 131 VYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWA 190
           ++ +    P      +G Q+ P++ +Q+TIFP   + IG S  HV  DG    HF+K W 
Sbjct: 130 LHGVLPKLPPPHVSPEGIQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKYWM 189

Query: 191 SI--FAGKE----LDAPLPFHD-RTIVQDPHGLESTFLRLQWS-IAGPINFYTEPINQRV 250
           S+   +GK+    L   LP H  R I++DP  + +  L   WS  +   + +  P N   
Sbjct: 190 SLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQNSAKHSSHVTPENM-- 249

Query: 251 GDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVASREG 310
              VRAT +LS+ QI+ LK+W   Q        E +SP +STFVVT + +WV  + +   
Sbjct: 250 ---VRATFTLSRKQIDNLKSWVTEQS-------ENQSP-VSTFVVTLAFIWVSLIKTLV- 309

Query: 311 GESELVCKDELYYFSFVANCRNGRLGY--PIPSGYFGNCLAFCIAAVKKSELLGENGMVM 370
            +SE   KDE+++     +CRN RL Y  PIP  YFGNC+A  I +VKK +LLGE  ++ 
Sbjct: 310 QDSETEAKDEVFHLMINVDCRN-RLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMA 369

Query: 371 GMKAIGKRVRELEKEAVKDIVEEMSIW----MKCVETESMVAVVGSPRLGAYDTDFGWGR 430
              AI  R++++      D+++    W     K V +    ++ G+P+LG YD DFG G+
Sbjct: 370 ASDAITARIKDM---LSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGK 429

Query: 431 PIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLF 468
           P K E+V I+ G   +  ESRD   G+E+G+AL    M  F ++ ++ +K F
Sbjct: 430 PCKMEIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKF 463

BLAST of HG10000960 vs. TAIR 10
Match: AT3G29680.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-62
Identity = 161/471 (34.18%), Postives = 245/471 (52.02%), Query Frame = 0

Query: 8   SVKIIEKCLVCPPPGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPHSTA-HFTNTILPS 67
           ++ +I+   V     SV    LPL++FD++WL+  P  R+ FYK   S+   F ++ILP 
Sbjct: 2   ALNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPK 61

Query: 68  LKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKD 127
           L+QSLSL L HF PLS +L   PQ  KPH++  P+D++ L + ES++   +       K+
Sbjct: 62  LEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYI----SSKE 121

Query: 128 AKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIK 187
            +   +L    PE +  SD      LL+LQIT+FP Q  SIG +  HV +DG     F K
Sbjct: 122 LRLETELRPLVPELQVSSDSAS---LLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHK 181

Query: 188 SWASI----FAGKELDAPLPFHDRTIVQDPHGLESTFLRLQWSIAGPINF----YTEPIN 247
           SWA I       ++ D P    DRT++  P GLE    +L   I+   ++       P  
Sbjct: 182 SWAHICKHGTTPQDFDLPTVL-DRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAK 241

Query: 248 QRVGDKVRATLSLSKDQIERLKNWGNSQMTKLPQPLEMESPYLSTFVVTCSLVWVCYVAS 307
           +   D VR TL L++  IE+LK    ++ T+          +LSTFVV+ + V  C V S
Sbjct: 242 EIDNDVVRVTLELTEVDIEKLKERAKNESTR-------SDLHLSTFVVSYAYVLTCMVKS 301

Query: 308 REGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNC-LAFCIAAVKKSELLGENGM 367
             G  +  V       F + A+ RN RL  P+P  YFGNC L       K +  LG++G 
Sbjct: 302 CGGDANRPV------RFMYAADFRN-RLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGY 361

Query: 368 VMGMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESM-VAVVGSPRLGAYDTDFGWGRP 427
           V G++ +   VR L    ++ I E      K ++ ++  V V GS + G Y +DFGWGRP
Sbjct: 362 VNGVEILSDSVRGLGSRNIESIWEVYEDGTKNMKLDTQNVTVTGSNQFGIYGSDFGWGRP 421

Query: 428 IKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLKLF 468
           +KT+V+ +     FS+   RDE GG+E+G++L    M  F++++  +  ++
Sbjct: 422 VKTDVMSLYKNNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of HG10000960 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-62
Identity = 145/443 (32.73%), Postives = 227/443 (51.24%), Query Frame = 0

Query: 29  LPLSYFDIIWLRHAPKCRLFFYKFPH-STAHFTNTILPSLKQSLSLTLQHFFPLSANLII 88
           LPL++FD++WL+H    R+ FYK    + + F + I+P+LK SLS +L H+ PL+ ++I 
Sbjct: 26  LPLTFFDLLWLKHKAVERVIFYKLTDVNRSLFDSVIVPNLKSSLSSSLSHYLPLAGHIIW 85

Query: 89  PPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPKDAKDVYQLFQDWPEFRFLSDGT 148
            P   KP ++YT  D++   +AES+S  F  L    P  + +++ L    PE +   D  
Sbjct: 86  EPHDPKPKIVYTQNDAVSFTVAESNS-DFSLLTGKEPFSSTELHPLV---PELQNSDDSA 145

Query: 149 QVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFIKSWASIFAGKELDAP---LPFH 208
            V+   + Q+T+FP Q   IG +  H   DG     F+KSWA +   ++   P   +PF+
Sbjct: 146 AVV---SFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHLCKHQDSSLPDDLIPFY 205

Query: 209 DRTIVQDPHGLESTFLRLQWSIAGPINFYTEPINQRVGDKVRATLSLSKDQIERLKNWGN 268
           DRT+++ P  +++  L++  SI  P +    P  +   D VR T  L+++ IE+L++   
Sbjct: 206 DRTVIKGPPEIDTKVLKIWHSIHKPKSLKLLPRPEIESDVVRYTFELTRENIEKLRD--- 265

Query: 269 SQMTKLP-QPLEMESPYLSTFVVTCSLVWVCYVASREGGESELVCKDELYYFSFVANCRN 328
               KL  +     S  LSTFV+T S V+ C + S  GG+      +    + F  +CR 
Sbjct: 266 ----KLKRESSSFSSVRLSTFVITFSYVFTCLIGS--GGDD----PNRPVGYRFAVDCRR 325

Query: 329 GRLGYPIPSGYFGNCLAFCI-AAVKKSELLGENGMVMGMKAIGKRVRELEKEAVKDIVEE 388
                PIP  YFGNC+   +   +     LGE G V+  + I   V EL+      I E 
Sbjct: 326 LIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEELDSNVAWKIPEL 385

Query: 389 MSIWMKCVETESMVAVVGSPRLGAYDTDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGI 448
           +  + K       V+V GS R G Y  DFGWG+P K+ +V ID     S+ ESRD  GG+
Sbjct: 386 LETYEKAPVDSQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQRGKISIAESRDGSGGV 445

Query: 449 EVGLALSSAYMTRFVAVWEQNLK 466
           E+G +L    M   + +  + +K
Sbjct: 446 EIGFSLKKQEMNVLIDLLHKGIK 448

BLAST of HG10000960 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-59
Identity = 149/479 (31.11%), Postives = 233/479 (48.64%), Query Frame = 0

Query: 8   SVKIIEKCLVCPP-PGSVPTTHLPLSYFDIIWLRHAPKCRLFFYKFPH-STAHFTNTILP 67
           S+K+I+   V P    S  +  LPL++FD++W +     R+ FYK    S   F + I+P
Sbjct: 9   SLKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVP 68

Query: 68  SLKQSLSLTLQHFFPLSANLIIPPQPQKPHLLYTPQDSIHLAIAESDSPHFHSLLPDHPK 127
           +LK SLS +L H+ PL+  L+  P   KP ++YTP D++   +AES++  F  L    P 
Sbjct: 69  NLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNA-DFSRLTGKEPF 128

Query: 128 DAKDVYQLFQDWPEFRFLSDGTQVIPLLTLQITIFPCQALSIGASFLHVGVDGPALHHFI 187
              ++Y L    PE     D    +   + Q+T+FP Q   I  +  H  +DG    +F+
Sbjct: 129 PTTELYPLV---PELHVSDDSASAV---SFQVTLFPNQGFCISVNAHHAVLDGKTTTNFL 188

Query: 188 KSWASIFAGKELDAP---LPFHDRTIVQDPHGLESTFLRLQWSIAGPINFYTEPINQR-- 247
           KSWA     ++   P   +P +DRT+++DP  L++  L     +A       EP N +  
Sbjct: 189 KSWARTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSL 248

Query: 248 -------VG-DKVRATLSLSKDQIERL-----KNWGNSQMTKLPQPLEMESPYLSTFVVT 307
                  +G D  R TL+L+++ I++L     K   +S ++  P+ L      LSTFV+ 
Sbjct: 249 KLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELR-----LSTFVIV 308

Query: 308 CSLVWVCYVASREGGESELVCKDELYYFSFVANCRNGRLGYPIPSGYFGNCLAFCIAAVK 367
            S    C + +R G  S  V       + F  +CR+  +  P+PS YFGNC++ C     
Sbjct: 309 YSYALTCLIKARGGDPSRPV------GYGFAVDCRS-LMVPPVPSSYFGNCVSACFKMSL 368

Query: 368 KSE-LLGENGMVMGMKAIGKRVRELEKEAVKDIVEEMSIWMKCVETESMVAVVGSPRLGA 427
            +E  + E G +   + +   V  L++     I E +  +        +++V GS R G 
Sbjct: 369 TAETFMSEEGFLAAARMVSDSVEALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGV 428

Query: 428 YDTDFGWGRPIKTEVVQIDNGRLFSLGESRDEKGGIEVGLALSSAYMTRFVAVWEQNLK 466
           Y  DFGWGRP K  VV ID G   S  ESRD  GG+E+G +L    M   V +  + L+
Sbjct: 429 YGLDFGWGRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512859.11.4e-18267.80coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_022140618.12.3e-13763.64coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Mom... [more]
XP_023533558.17.2e-12349.14coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_022971000.11.4e-12148.71coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
XP_022947400.14.0e-12148.50coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cuc... [more]
Match NameE-valueIdentityDescription
Q9ZWB45.1e-7937.58Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabid... [more]
Q9LR733.3e-7837.71Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabid... [more]
Q9ZWR81.3e-7135.53Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1[more]
Q589Y09.6e-7034.18Phenolic glucoside malonyltransferase 1 OS=Nicotiana tabacum OX=4097 GN=mat1 PE=... [more]
Q8GSN83.2e-6535.32Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase OS=Dahlia ... [more]
Match NameE-valueIdentityDescription
A0A6J1CIE11.1e-13763.64coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=M... [more]
A0A6J1I4H86.6e-12248.71coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A6J1G6S51.9e-12148.50coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=C... [more]
A0A1Q3B9H94.3e-12149.68Transferase domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3... [more]
A0A6J1CHV47.3e-12148.74LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltr... [more]
Match NameE-valueIdentityDescription
AT1G03940.13.6e-8037.58HXXXD-type acyl-transferase family protein [more]
AT1G03495.12.3e-7937.71HXXXD-type acyl-transferase family protein [more]
AT3G29680.11.2e-6234.18HXXXD-type acyl-transferase family protein [more]
AT5G39090.14.4e-6232.73HXXXD-type acyl-transferase family protein [more]
AT5G39050.12.1e-5931.11HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 8..230
e-value: 6.3E-57
score: 194.6
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 242..467
e-value: 1.2E-45
score: 157.3
IPR003480TransferasePFAMPF02458Transferasecoord: 8..458
e-value: 7.0E-42
score: 143.6
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 9..467
NoneNo IPR availablePANTHERPTHR31625:SF52COUMAROYL-COA:ANTHOCYANIDIN 3-O-GLUCOSIDE-6''-O-COUMAROYLTRANSFERASE 1-RELATEDcoord: 9..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000960.1HG10000960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups