HG10000958 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000958 vs. NCBI nr
Match: XP_038901814.1 (uncharacterized GPI-anchored protein At4g28100-like [Benincasa hispida])

HSP 1 Score: 545.4 bits (1404), Expect = 3.0e-151
Identity = 281/304 (92.43%), Postives = 289/304 (95.07%), Query Frame = 0

Query: 1   MISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQA 60
           MISL LILAT+LLISTTLFPISVA+L+RG+FSVPAFPV+TE Q CRLDLSDELFGGVSQA
Sbjct: 1   MISLMLILATILLISTTLFPISVAVLVRGQFSVPAFPVETETQTCRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAP PSAEIDLPLMPDDSQKCVESLQSSL R
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPTPSAEIDLPLMPDDSQKCVESLQSSLAR 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNAS 180
           RNI+IPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPT AVRDLE NC+NAS
Sbjct: 121 RNIKIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTAAVRDLEKNCQNAS 180

Query: 181 YSGCTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKG  D STTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 181 YSGCTKCLGALQKVKGVRDSSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240

Query: 241 IMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLVCLLFV 300
           IMYSAHPPHNSKCSPDQENMPLAVD LQF+DAHSQSQ SSI M PFLRLLLTPLVCLL V
Sbjct: 241 IMYSAHPPHNSKCSPDQENMPLAVDYLQFDDAHSQSQ-SSIFMLPFLRLLLTPLVCLL-V 300

Query: 301 IVWP 305
           IVWP
Sbjct: 301 IVWP 302

BLAST of HG10000958 vs. NCBI nr
Match: XP_004139964.1 (uncharacterized GPI-anchored protein At4g28100 [Cucumis sativus] >KGN46678.1 hypothetical protein Csa_020848 [Cucumis sativus])

HSP 1 Score: 534.3 bits (1375), Expect = 7.0e-148
Identity = 269/303 (88.78%), Postives = 284/303 (93.73%), Query Frame = 0

Query: 4   LTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQACNG 63
           ++++    L+ISTTLFPISVA+ +RG++SVPAFPV+TENQ CRLDLSDELFGGVSQACNG
Sbjct: 2   ISILAIATLVISTTLFPISVAVSVRGQYSVPAFPVETENQACRLDLSDELFGGVSQACNG 61

Query: 64  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLRRNI 123
           NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSL+RRNI
Sbjct: 62  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNI 121

Query: 124 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNASYSG 183
           RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PT AVRDLETNCRNASYSG
Sbjct: 122 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSG 181

Query: 184 CTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 243
           CTKCLGALQKVKGAGDRSTT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY
Sbjct: 182 CTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 241

Query: 244 SAHPPHNSKCSPDQENMPLAVDSLQFEDAH--SQSQSSSILMFPFLRLLLTPLVCLLFVI 303
           SAHPPHNS CSPDQENMPLAVDSLQF+DAH  S SQ SSI +F FLRLLLTP VCL  V 
Sbjct: 242 SAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLLLTPFVCLFLVK 301

Query: 304 VWP 305
           +WP
Sbjct: 302 IWP 304

BLAST of HG10000958 vs. NCBI nr
Match: XP_008448226.1 (PREDICTED: uncharacterized GPI-anchored protein At4g28100-like [Cucumis melo] >TYJ96632.1 putative GPI-anchored protein [Cucumis melo var. makuwa])

HSP 1 Score: 530.8 bits (1366), Expect = 7.8e-147
Identity = 268/302 (88.74%), Postives = 282/302 (93.38%), Query Frame = 0

Query: 4   LTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQACNG 63
           ++++     LISTTLFPISVA+++RG+FSVPAFPV+TENQ CRLDLSDELFGGVSQACNG
Sbjct: 2   ISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNG 61

Query: 64  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLRRNI 123
           NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSL+RRNI
Sbjct: 62  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNI 121

Query: 124 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNASYSG 183
           RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PTTAVRDLETNCRNASYSG
Sbjct: 122 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYSG 181

Query: 184 CTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 243
           CTKCLGALQKVKGAGDRSTT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY
Sbjct: 182 CTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 241

Query: 244 SAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSS-SILMFPFLRLLLTPLVCLLFVIV 303
           SAH PHNS CSPDQENMPLAVDSLQF+DAHS S S  SI MF F RL+LTP VCL  V +
Sbjct: 242 SAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSIFMFSFFRLVLTPFVCLFLVKI 301

Query: 304 WP 305
           WP
Sbjct: 302 WP 303

BLAST of HG10000958 vs. NCBI nr
Match: KAG7034939.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 245/295 (83.05%), Postives = 267/295 (90.51%), Query Frame = 0

Query: 1   MISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQA 60
           M+SL ++++++L+I +TLF +SVA  + G  SVPA PV+ E QVCRLDLSDELFGGVSQA
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SL++
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NATPT+AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 180

Query: 181 YSGCTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D STTERA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 181 YSGCTKCLGALQKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240

Query: 241 IMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLV 296
           IMYSAHPPH+SKCSPDQENMPLAVDSLQFE+  S + S S  M+PFL L   P +
Sbjct: 241 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFI 294

BLAST of HG10000958 vs. NCBI nr
Match: XP_022947263.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita moschata])

HSP 1 Score: 486.5 bits (1251), Expect = 1.7e-133
Identity = 246/298 (82.55%), Postives = 268/298 (89.93%), Query Frame = 0

Query: 1   MISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQA 60
           M+SL ++++++L+I +TLF +SVA  + G  SVPA PV+ E QVCRLDLSDELFGGVSQA
Sbjct: 106 MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 165

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SL++
Sbjct: 166 CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 225

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NATPT+AVRDLE NCRNAS
Sbjct: 226 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 285

Query: 181 YSGCTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D STTERA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 286 YSGCTKCLGALQKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 345

Query: 241 IMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLVCLL 299
           IMYSAHPPH+SKCSPDQENMPLAVDSLQFE+  S + S S  M+PFL L   P +  L
Sbjct: 346 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFL 402

BLAST of HG10000958 vs. ExPASy Swiss-Prot
Match: Q9SUC9 (Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 GN=At4g28100 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.0e-106
Identity = 206/303 (67.99%), Postives = 245/303 (80.86%), Query Frame = 0

Query: 2   ISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQAC 61
           ++L ++L   LL ST L  + V  +     +VPAFPV+T+ Q CRLDLS+ELFGGV++AC
Sbjct: 5   LTLLILLLCSLLFSTVLSNLLVEPVQPN--TVPAFPVETQAQSCRLDLSNELFGGVNEAC 64

Query: 62  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP---SAEIDLPLMPDDSQKCVESLQSSL 121
             NLDRSRCCPVLAAWLFAAHARSAL++ APAP   S++ D P+ PDDSQKCV +LQS+L
Sbjct: 65  GRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVNTLQSAL 124

Query: 122 LRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNL-SGFQNATPTTAVRDLETNCR 181
           L + I+IPQPNSSCDA+LCFCGIRLHQISSLSCPAAFN+ SGF+NATPT AV++LE  CR
Sbjct: 125 LTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNATPTAAVKNLEKECR 184

Query: 182 NASYSGCTKCLGALQ--KVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVS 241
           N+SYSGCT+CLGALQ  KV+G   ++TTER  KM ++DCQLMGLTWLLARNKTAYIPTVS
Sbjct: 185 NSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQLMGLTWLLARNKTAYIPTVS 244

Query: 242 AVLRAIMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLV 299
           AVLRAIMYS HPPH +KCSPDQENMPLAVDSLQF+ + S S S    + PFL L+L   +
Sbjct: 245 AVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFS-SSSHLFGVLPFLPLVLCIFL 304

BLAST of HG10000958 vs. ExPASy TrEMBL
Match: A0A0A0KAY3 (SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.4e-148
Identity = 269/303 (88.78%), Postives = 284/303 (93.73%), Query Frame = 0

Query: 4   LTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQACNG 63
           ++++    L+ISTTLFPISVA+ +RG++SVPAFPV+TENQ CRLDLSDELFGGVSQACNG
Sbjct: 2   ISILAIATLVISTTLFPISVAVSVRGQYSVPAFPVETENQACRLDLSDELFGGVSQACNG 61

Query: 64  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLRRNI 123
           NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSL+RRNI
Sbjct: 62  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNI 121

Query: 124 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNASYSG 183
           RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PT AVRDLETNCRNASYSG
Sbjct: 122 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNASYSG 181

Query: 184 CTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 243
           CTKCLGALQKVKGAGDRSTT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY
Sbjct: 182 CTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 241

Query: 244 SAHPPHNSKCSPDQENMPLAVDSLQFEDAH--SQSQSSSILMFPFLRLLLTPLVCLLFVI 303
           SAHPPHNS CSPDQENMPLAVDSLQF+DAH  S SQ SSI +F FLRLLLTP VCL  V 
Sbjct: 242 SAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSSIFIFSFLRLLLTPFVCLFLVK 301

Query: 304 VWP 305
           +WP
Sbjct: 302 IWP 304

BLAST of HG10000958 vs. ExPASy TrEMBL
Match: A0A5D3B9U0 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00450 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.8e-147
Identity = 268/302 (88.74%), Postives = 282/302 (93.38%), Query Frame = 0

Query: 4   LTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQACNG 63
           ++++     LISTTLFPISVA+++RG+FSVPAFPV+TENQ CRLDLSDELFGGVSQACNG
Sbjct: 2   ISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNG 61

Query: 64  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLRRNI 123
           NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSL+RRNI
Sbjct: 62  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNI 121

Query: 124 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNASYSG 183
           RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PTTAVRDLETNCRNASYSG
Sbjct: 122 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYSG 181

Query: 184 CTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 243
           CTKCLGALQKVKGAGDRSTT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY
Sbjct: 182 CTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 241

Query: 244 SAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSS-SILMFPFLRLLLTPLVCLLFVIV 303
           SAH PHNS CSPDQENMPLAVDSLQF+DAHS S S  SI MF F RL+LTP VCL  V +
Sbjct: 242 SAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSIFMFSFFRLVLTPFVCLFLVKI 301

Query: 304 WP 305
           WP
Sbjct: 302 WP 303

BLAST of HG10000958 vs. ExPASy TrEMBL
Match: A0A1S3BJT3 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=LOC103490480 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.8e-147
Identity = 268/302 (88.74%), Postives = 282/302 (93.38%), Query Frame = 0

Query: 4   LTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQACNG 63
           ++++     LISTTLFPISVA+++RG+FSVPAFPV+TENQ CRLDLSDELFGGVSQACNG
Sbjct: 2   ISILAIATFLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQACNG 61

Query: 64  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLRRNI 123
           NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSL+RRNI
Sbjct: 62  NLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVRRNI 121

Query: 124 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNASYSG 183
           RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNA+PTTAVRDLETNCRNASYSG
Sbjct: 122 RIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNASYSG 181

Query: 184 CTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 243
           CTKCLGALQKVKGAGDRSTT RARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY
Sbjct: 182 CTKCLGALQKVKGAGDRSTTARARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMY 241

Query: 244 SAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSS-SILMFPFLRLLLTPLVCLLFVIV 303
           SAH PHNS CSPDQENMPLAVDSLQF+DAHS S S  SI MF F RL+LTP VCL  V +
Sbjct: 242 SAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSIFMFSFFRLVLTPFVCLFLVKI 301

Query: 304 WP 305
           WP
Sbjct: 302 WP 303

BLAST of HG10000958 vs. ExPASy TrEMBL
Match: A0A6J1G647 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=3662 GN=LOC111451182 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 8.1e-134
Identity = 246/298 (82.55%), Postives = 268/298 (89.93%), Query Frame = 0

Query: 1   MISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQA 60
           M+SL ++++++L+I +TLF +SVA  + G  SVPA PV+ E QVCRLDLSDELFGGVSQA
Sbjct: 106 MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 165

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SL++
Sbjct: 166 CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 225

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NATPT+AVRDLE NCRNAS
Sbjct: 226 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 285

Query: 181 YSGCTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D STTERA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 286 YSGCTKCLGALQKVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 345

Query: 241 IMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLVCLL 299
           IMYSAHPPH+SKCSPDQENMPLAVDSLQFE+  S + S S  M+PFL L   P +  L
Sbjct: 346 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFL 402

BLAST of HG10000958 vs. ExPASy TrEMBL
Match: A0A6J1HZY7 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 GN=LOC111469629 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.9e-133
Identity = 246/300 (82.00%), Postives = 269/300 (89.67%), Query Frame = 0

Query: 1   MISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQA 60
           M+SL ++++++L+I +TLF +SVA  + G  SVPA PV+ E QVCRLDLSDELFGGVSQA
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLLR 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SL++
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATPTTAVRDLETNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NATPT AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNAS 180

Query: 181 YSGCTKCLGALQKVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240
           YSGCTKCLGALQKVKGA D +TTERA KMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA
Sbjct: 181 YSGCTKCLGALQKVKGARDTNTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRA 240

Query: 241 IMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLVCLLFV 300
           IMYSAHPPH+SKCSPDQENMPLAVDSLQFE+  S + S S  M+PFL     PL  +LF+
Sbjct: 241 IMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFL-----PLTAVLFI 294

BLAST of HG10000958 vs. TAIR 10
Match: AT4G28100.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G18050.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 384.8 bits (987), Expect = 6.4e-107
Identity = 206/303 (67.99%), Postives = 245/303 (80.86%), Query Frame = 0

Query: 2   ISLTLILATVLLISTTLFPISVALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVSQAC 61
           ++L ++L   LL ST L  + V  +     +VPAFPV+T+ Q CRLDLS+ELFGGV++AC
Sbjct: 5   LTLLILLLCSLLFSTVLSNLLVEPVQPN--TVPAFPVETQAQSCRLDLSNELFGGVNEAC 64

Query: 62  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP---SAEIDLPLMPDDSQKCVESLQSSL 121
             NLDRSRCCPVLAAWLFAAHARSAL++ APAP   S++ D P+ PDDSQKCV +LQS+L
Sbjct: 65  GRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVNTLQSAL 124

Query: 122 LRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNL-SGFQNATPTTAVRDLETNCR 181
           L + I+IPQPNSSCDA+LCFCGIRLHQISSLSCPAAFN+ SGF+NATPT AV++LE  CR
Sbjct: 125 LTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNATPTAAVKNLEKECR 184

Query: 182 NASYSGCTKCLGALQ--KVKGAGDRSTTERARKMFNRDCQLMGLTWLLARNKTAYIPTVS 241
           N+SYSGCT+CLGALQ  KV+G   ++TTER  KM ++DCQLMGLTWLLARNKTAYIPTVS
Sbjct: 185 NSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQLMGLTWLLARNKTAYIPTVS 244

Query: 242 AVLRAIMYSAHPPHNSKCSPDQENMPLAVDSLQFEDAHSQSQSSSILMFPFLRLLLTPLV 299
           AVLRAIMYS HPPH +KCSPDQENMPLAVDSLQF+ + S S S    + PFL L+L   +
Sbjct: 245 AVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFS-SSSHLFGVLPFLPLVLCIFL 304

BLAST of HG10000958 vs. TAIR 10
Match: AT3G18050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G28100.1); Has 67 Blast hits to 66 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 192.2 bits (487), Expect = 6.1e-49
Identity = 127/336 (37.80%), Postives = 188/336 (55.95%), Query Frame = 0

Query: 2   ISLTLILATVLLISTTLFPISV---ALLIRGRFSVPAFPVDTENQVCRLDLSDELFGGVS 61
           ISL +I    +L   +  P++V    +      ++PAFP  ++   C LDL ++LF G+ 
Sbjct: 9   ISLIIIFLFTILSPVSPEPVTVQPFRVKSSPPATIPAFPEQSDFSGCPLDLPEDLFHGIK 68

Query: 62  QACNG-NLDRSRCCPVLAAWLFAAH------------ARSALEVSAPAPSAEIDLPLMPD 121
            AC+G  L + +CCPVL AWL++A+            AR++   SA   + E D+PL+PD
Sbjct: 69  SACSGKKLHKGKCCPVLGAWLYSAYSTTALSRSISAAARNSSSSSAAVTTPEEDMPLLPD 128

Query: 122 DSQKCVESLQSSLLRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNATP 181
           DS+ CV+ L  SL +R I + +PN +CD V C+CGIRLH    LSC  AF ++       
Sbjct: 129 DSETCVDGLGKSLRQRGIELTRPNETCDVVYCYCGIRLH---PLSCSEAFRVNDEGRLVG 188

Query: 182 TTAVRDLETNCRNASYS---------GCTKCLGALQKV---KGAGDRSTTE----RARKM 241
              V  LET+C + S++         GC KCL +L K+   K +G R+ ++    R  KM
Sbjct: 189 DERVDRLETDCLSGSHNNADGFSPLLGCNKCLNSLYKLNPKKTSGTRNPSKEDRNRTAKM 248

Query: 242 FNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHNSKCSPDQENMPLAVDSLQF 301
            N+DC LMGLTWLLA+N+TAY PTV++VLRA+M + H      C+   + MPLAVDS +F
Sbjct: 249 HNKDCVLMGLTWLLAKNRTAYFPTVTSVLRAVMLN-HDGVPRSCALGSDGMPLAVDSSEF 308

Query: 302 EDAHSQSQSSSILMFP--FLRLLLTPLVCLLFVIVW 304
            +      S + L +P   +  LL  ++ L+ +  W
Sbjct: 309 SNG-----SPTSLQYPHHLVHFLLYSVITLVLIRSW 335