HG10000922 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000922
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMechanosensitive ion channel protein
LocationChr09: 11608837 .. 11611786 (+)
RNA-Seq ExpressionHG10000922
SyntenyHG10000922
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGAAGCAGTACACAAATCTGATTGGAAAGAGGTTATCGTCAAGGTCGATCAGGACGATATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGAAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAGTAAGTTAATTCATTTCATACAGCCTAATACTGCTATCGTTTGAATTGACTGTTTAAAAAGTGTTATTGTAAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTTTTATTATTACTATGAAACGACTGGATAAATTGTGTTTTATGAAAAAATGTTCATTCAAAATTGTGGCTAGCTCCAATTAATGAACCTTTTACTCCATTCTTAATTTTCCCCCCTTTGTTAGATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGGTAAGAGCTATCTTATCAAAGTCTTAAATATATTTTTATGTTCTCATTCCACATGTTATTGTTGCAAATTCAATATGGGTGTTGTCTTGATATTAAATTAGTGTTGGTCTAATGATATTATACCTAAAAATTAAAATTGTTGCAGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGGTAATTCTTCTCTTCAACTAATTATTTTAATTCTACATTCCCATCTCATATTCTTTGATTTAAACTGTAGTTCCTTCAAATTTCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGGTATGTGGAGAAAAGAAAACACAGTTTACTAAAAATTCATTTTATTATTGTTTTTTATTTTTTTTTTATTTTTCTTTGTCTTTGCAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

mRNA sequence

ATGGTGGAAGCAGTACACAAATCTGATTGGAAAGAGGTTATCGTCAAGGTCGATCAGGACGATATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGAAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

Coding sequence (CDS)

ATGGTGGAAGCAGTACACAAATCTGATTGGAAAGAGGTTATCGTCAAGGTCGATCAGGACGATATGTCGGGTAATGCTTCGTTGAGAGGTATGAAGTTGGTGAACACCAGTGGGAAGATTTGGCAGGAGTCCAGCAATGATTTCTGGAATGATAGTGATAATAGACTAAATAATGGCGAAGTTACGAATGGTAATGGTGGTGGTGGTAGGAGTGAGGATTTTGAGCTTAGGCAACAAAGGAAGGATGTGGAAGATCCGCCGTCGAAGCTTATTGGACAGTTGCTTCACAAGAAGAAGGGGTCCGGAGAGATATCTTTAGATATGGATATGGAAATGCTGGAACTACCACAGGATAAGACTCTGCTTCCTATGGTCGCGGATTCTCCGATGCGAAGACGGCACAGAGACTCTCTGCCGGTTGATGAAGAACATAGAGGAAAGCAACCGCAGCAGCGTGATCTTCGAGCGCATGGCAGCAATGGTGAAGATGATGGTGGTGCTGAGGTTTTGAGATGCACATCTAATTCGTCCTTTGGAAGAGATGTGTCGTTCCAGAGGAAATCAGGGCAAGTTCGCCGTGGTTTACTGTCAAAAGCCATAGATGAGGAAGACGATGATCCATTCTTGGAAGAAGATGTTCCTGATAAGTATAAGAAGGGTAATCTAGGTGTTTTGACTCTGTTACAGTGGGCGAGTTTGATTTTGATAATCGCAGCATTGATTTGCACTTTGGCAATTCGTAACTGGAGGAGAAAGAAACTATGGAAGCTTGAACTATGGAAATGGGAGGTTATGATTCTTGTTCTGATATGTGGAAGGTTGGTTTCTGGCTGGGGGATTCGGATAATTGTCTTCTTCATTGAAAGAAACTTTCTACTGCGTAAGAGGGTTCTATACTTTGTTTATGGAATCAGAAAGGCAGTCCAGAATTGTCTTTGGTTGGGTCTAGTTTTAATTGCTTGGAACTTCCTGTTTGATGACAAAGTTCAAAGAGAGGTAAAGAGCAATGCCTTAGAATATGTGACCAAAGTATTGGTATGTCTTCTTGTAAGCACTTTGGTTTGGCTAGTAAAAACGCTAATGGTGAAGGTCTTGGCCTCTTTTTTCCATGTGAGTACATATTTTGATAGGATTCAAGCTGCATTGTTTGACCAATATGTAATTGGGACACTGTTGGGGCCTCCATTGATGGAGATTTCAAAGAATGAGGAAGAAGAAGAGAGGGTTGCAGAAGAGGTTATAAAGTTAGAGAATGCAGGGGCTACCATCCCTCCTGATTTAAAGGCAACAGCTCAAAAGGGTGGGAGGGTGATAGGGGAGGGTATAACAATTGATCACTTGCATAAGCTTAGTCCTAAGAATGTGTCTGCCTGGAATATGAAGAGGTTGATGAAAATCATTAGACATGTGACTCTTTCAACTTTGGATGAACAAATCAAAGATTCAATAACTCATGAGAACGAATCAACCACACAGATCAAGAGTGGATATGAGGCAAAAGTTGCTGCTAAGAAGATTTTTCTGAACGTGGCTAGGCATGGTTCTAAATATATCTACGTAGAGGACTTGAAGCGTTTCATGGACGAAGATAAGGCTTCAAAAACCATGGGACTGTTTGAAGAAGCACGTGAGAGTAGGAAAATAAGCAAATCGTCATTGAAAAACTGGGTGATTTATGCCTCTAGGGAACGAACGGCTCTAGCTTCGACATTAGACGATTCCAAAACAGCTGTGAAAAAACTTCACCGCATGGTGAACATTTTAATTTCTGTCATTATTCTGGTTATTTGGCTATTGATTCTTGGTATAGCCACTAGCAAATTTCTCCTATTCGCGACCTCTCAACTTGTCCTTGTCGCATTTGTATTTGGAAATACTTGCAAGACTGTTTTTGAATCAATCATCTTCTTATTTGTAATGCATCCATTTGATGTTGGAAACCTATGCGAAATCAATGGTGTGCAGATGATTGTAAAAGAAATGAACATTTTGACGACCATTTTTCTAAGATATGACAACCAGAAGATCATAATTCCGAACAGTCTTCTCGCGACAATGGCTATCCACAACTTCTATCGTAGTCCTGACATGGGAGATGCTGTAGAATTCTGTCTTCACATATGTACTCCGCCTGAGAAAATTGCTATTATGAGACAAAGAATTATAAGTTACATTGAGGGCAAGAAAAAGCATTGGTGTCCTGCGCCAAGGATTTTCCTCAAGGATGTAGAAGAGTTAAACAGAATCAGAATAGCAATATGTCTTACTCATAGAATGAACCATCAAGACATGGGCAAGAGGTGGACTAGGAGAGCTCTTTTGGTCGAAGAACTCGTTAAAATTTTCCAAGAGATCGACTTGCAATACCGTCTCTCAGGGGGCTAG

Protein sequence

MVEAVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGGGGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVADSPMRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTSNSSFGRDVSFQRKSGQVRRGLLSKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATAQKGGRVIGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKIFQEIDLQYRLSGG
Homology
BLAST of HG10000922 vs. NCBI nr
Match: XP_038900754.1 (mechanosensitive ion channel protein 6-like [Benincasa hispida])

HSP 1 Score: 1264.6 bits (3271), Expect = 0.0e+00
Identity = 683/864 (79.05%), Postives = 726/864 (84.03%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVDQDD    ASLR  +LVNTSG+IW+ESS DFWNDSDNR +NG+       
Sbjct: 49  SDRKEVIVKVDQDD----ASLRAKELVNTSGRIWRESSYDFWNDSDNRRDNGDCV----- 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
            GRSEDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKTLLP VA+
Sbjct: 109 -GRSEDFEFRQDRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLLPAVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDSLPVDEEHRG+QP+QRD RA+GSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDSLPVDEEHRGQQPKQRDRRAYGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR-----------------------------KSGQVRRGL 247
           G AEVLRCTSNSSF RDVSFQR                             KSGQVR GL
Sbjct: 229 GAAEVLRCTSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEYQDRRSGRVPTKSGQVRSGL 288

Query: 248 LSKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLW 307
           LSKA+DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAALICTL IR WRRKKLW
Sbjct: 289 LSKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALICTLTIRYWRRKKLW 348

Query: 308 KLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLG 367
           KLELWKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLG
Sbjct: 349 KLELWKWEVMILVLICGRLVSGWGIRLIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLG 408

Query: 368 LVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYF 427
           LVLIAWNFLFDDKVQREVKSNALEYVTKVL+CLLVSTLVWLVKTLMVKVLAS FHVSTYF
Sbjct: 409 LVLIAWNFLFDDKVQREVKSNALEYVTKVLICLLVSTLVWLVKTLMVKVLASSFHVSTYF 468

Query: 428 DRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATA---- 487
           DRIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEVIKL+NAGATIPPDLKATA    
Sbjct: 469 DRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTT 528

Query: 488 QKGGRVIG------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           QKGGRVIG                        +GITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 QKGGRVIGSGGLQKSPRGRSGKLSRTLSKKGDDGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH  LSTLDEQIKD+ T E+ESTTQIKS YEAKVAAKKIFLNVAR GSKYIY+EDL RFM
Sbjct: 589 RHGALSTLDEQIKDA-TDEDESTTQIKSEYEAKVAAKKIFLNVARQGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A E+RKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACENRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+S+IILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSIIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTT FLRYDNQKII PNSLLAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTSFLRYDNQKIIFPNSLLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
           CTPPEKIAIM+QRIISYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 CTPPEKIAIMKQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. NCBI nr
Match: XP_008448342.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1241.5 bits (3211), Expect = 0.0e+00
Identity = 674/864 (78.01%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. NCBI nr
Match: TYK23963.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1241.5 bits (3211), Expect = 0.0e+00
Identity = 674/864 (78.01%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. NCBI nr
Match: KAA0061966.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 673/864 (77.89%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKV++ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVNEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. NCBI nr
Match: XP_004140046.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 672/864 (77.78%), Postives = 718/864 (83.10%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   +LR   LVN SG IW+ESS DFWNDSDNR N GE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---TLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              ++DFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNS F RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SKA+DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEVIKL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTT+IKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD +EFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRIISYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 1.1e-238
Identity = 465/850 (54.71%), Postives = 601/850 (70.71%), Query Frame = 0

Query: 4   AVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTN 63
           AV  +D +EVIVK+D ++   N  + G     T GKIW++ S DFW D +  LN G    
Sbjct: 2   AVDAADRREVIVKIDGEN-GNNNGVSG----ETVGKIWRDGSYDFWTDGEGNLNKGHNAA 61

Query: 64  G---------NGGGGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLE 123
                      G   + E FE R+     EDPP+KLIGQ LHK++ SGEI LDMD+ M E
Sbjct: 62  AVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDE 121

Query: 124 LPQDKTLLPMVADSPMRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTS 183
           L Q + L P V++SP     RD  PV          +RD R++ +N  DDG  EV++C+ 
Sbjct: 122 L-QSRGLTP-VSESPRVSTKRD--PVG---------RRDSRSN-TNNNDDG--EVVKCSG 181

Query: 184 NSS----------------------------FGRDVSFQR--KSGQVRRGLLSKAI---- 243
           N++                               D+   R  KSGQ++ G   K+     
Sbjct: 182 NNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQG 241

Query: 244 DEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELW 303
           +EE+DDPF  ED+P++Y+K  L +  +L+W SLILIIA  +CTLAI + R+KKLW+L+LW
Sbjct: 242 EEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLW 301

Query: 304 KWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIA 363
           KWE M+LVLICGRLVS W ++I+VFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVL+A
Sbjct: 302 KWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLA 361

Query: 364 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQA 423
           W+FLFD+KV +   + AL  VTK+ VCLLV  L+WLVKTL+VKVLAS FH+STYFDRIQ 
Sbjct: 362 WHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQE 421

Query: 424 ALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATAQK------- 483
           +LF QYVI TL GPPL+EI KNEEEEER++ EV K +N G     ++++ AQK       
Sbjct: 422 SLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGV---EIQSGAQKSPMKTGK 481

Query: 484 ------------GGRVIGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKD 543
                       GG    +GITID LHKL+PKNVSAW MKRLM IIR+ +L+TLDEQ++D
Sbjct: 482 SPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQD 541

Query: 544 SITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEE 603
             + +++   QI+S +EAK+AA+KIF NVA+ GSK+IY  D+ RF+ +D+A KT+ LFE 
Sbjct: 542 P-SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEG 601

Query: 604 ARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLIL 663
           A E+ +ISKSSLKNWV+ A RER ALA TL+D+KTAV +LH+MVNI++ +IILVIWL+IL
Sbjct: 602 ASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIIL 661

Query: 664 GIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEM 723
           GI ++KFL+  +SQ+V+VAF+FGN CK VFESII+LFV+HPFDVG+ CEI+GVQM+V+EM
Sbjct: 662 GITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEM 721

Query: 724 NILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRI 783
           NILTT+FLR+DNQK++ PNSLL T +I N+YRSPDMGD +EF +HI TP EKI +++QRI
Sbjct: 722 NILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRI 781

Query: 784 ISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKI 792
            SYIEGKK HW PAP I  KD+E LN +RIA+  THRMNHQDMG++W RR+ LVEE+ KI
Sbjct: 782 TSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKI 822

BLAST of HG10000922 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 8.6e-233
Identity = 452/851 (53.11%), Postives = 593/851 (69.68%), Query Frame = 0

Query: 4   AVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTN 63
           AV  +D ++ IV ++  + SG     G       G IW+ESS DFW D +   N+ +  +
Sbjct: 3   AVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGDD 62

Query: 64  GNGGGGRSEDFELRQQRK----DVEDPPSKLIGQLLHKKKGSG-EISLDMDMEMLELPQD 123
            +  GG S  F  R +R+    ++ DPPSKLIGQ LHK++ SG EISLD+++ M EL  +
Sbjct: 63  EDEDGG-SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 122

Query: 124 KTLLPMVADSPMRRR----HRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGG-----AEV 183
               P  A +  RR        S PV  + +    ++R  R       D+ G     AEV
Sbjct: 123 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDEAEV 182

Query: 184 LRCTS-------NSSFGR---------------DVSFQRKSGQVRRGLLSKA-------- 243
           L+C S       N +  R               ++   R+SG  + G L K+        
Sbjct: 183 LKCGSKKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGR 242

Query: 244 ---IDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 303
               +EE++DPFL+ED+P+++K+  L     L+W SL+LI+ +L+C+L I N +RK  WK
Sbjct: 243 NGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWK 302

Query: 304 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 363
           L+LWKWEV +LVLICGRLVS W +RIIVF +E+NF  RKRVLYFVYG+RK+VQNCLWLGL
Sbjct: 303 LDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGL 362

Query: 364 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 423
           VL+AW+FLFD KV+RE +S AL YVT+VLVCLLV+ ++WLVKT++VKVLAS FH+STYFD
Sbjct: 363 VLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 422

Query: 424 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLEN-AGATIPPDLKATAQKGG 483
           RIQ +LF QYVI TL GPPLMEI + EEEE++VAE+V  LE  AGA +PP LKAT +   
Sbjct: 423 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 482

Query: 484 RV---------------IGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIK 543
           +V                GEGI ID L +++ KNVSAWNMKRLM II    +STLD+ ++
Sbjct: 483 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 542

Query: 544 DSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFE 603
           D+ T E+E  T I+S YEAK AA+KIF NV   GS+YIY+ED  RF+ E++A + M LFE
Sbjct: 543 DT-TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 602

Query: 604 EARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLI 663
            A ES KISKS LKNWV+ A RER ALA TL+D+KTAV +LHR++N++I +II++IWLLI
Sbjct: 603 GASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 662

Query: 664 LGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKE 723
           LGIAT++FLL  +SQL+LVAFVFGN+CKT+FE+IIFLFVMHPFDVG+ CEI+GVQ++V+E
Sbjct: 663 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 722

Query: 724 MNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQR 783
           MNILTT+FLRYDNQKII PNS+L T  I N+YRSPDMGDAVEFC+HI TPPEKI  ++QR
Sbjct: 723 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 782

Query: 784 IISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVK 792
           I+SY++ KK +W PAP I    +++LN ++IA+ LTHRMNHQDMG+R+ RR LL+EE+ K
Sbjct: 783 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 842

BLAST of HG10000922 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 796.6 bits (2056), Expect = 2.6e-229
Identity = 407/661 (61.57%), Postives = 529/661 (80.03%), Query Frame = 0

Query: 168 EVLRCTSNSSFGRDVSFQR----------------------KSGQVRRGLLSKAIDEEDD 227
           EV+RCTSN SF R                            +SGQ++ GLL+  IDEE D
Sbjct: 222 EVVRCTSNMSFQRKSELISRVKTRSRLQDPPREEETPYSGWRSGQLKSGLLAD-IDEE-D 281

Query: 228 DPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELWKWEVM 287
           DP  EEDVPD+YK+G L  +TLLQW SL+ IIAAL C+L+I++W++ ++W L LWKWEV 
Sbjct: 282 DPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVF 341

Query: 288 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIAWNFLF 347
           +LVLICGRLVSGWGIRI+VFFIERNFLLRKRVLYFVYG+R+AVQNCLWLGLVL+AW+FLF
Sbjct: 342 LLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLF 401

Query: 348 DDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQAALFDQ 407
           D KVQRE +S  L YVTK+LVC L+ST++WL+KTL+VKVLAS FHVSTYFDRIQ ALF+Q
Sbjct: 402 DKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQ 461

Query: 408 YVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATA---QKGGRVIG--- 467
           YVI TL GPP++E+S+ EEEEER  +E+ K++NAGA +PPDL A A    K GRV+    
Sbjct: 462 YVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVMNPKL 521

Query: 468 ----------EGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKDSITHENES 527
                      GI+++HLH+++ KN+SAWNMKRLMKI+R+V+L+TLDEQ+ +S T+E+ES
Sbjct: 522 SPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLES-TYEDES 581

Query: 528 TTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEEARESRKIS 587
           T QI+S  EAK AA+KIF NV + G+KYIY+EDL RF+ ED+A KTMGLFE A E+++IS
Sbjct: 582 TRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRIS 641

Query: 588 KSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLILGIATSKFL 647
           KS+LKNW++ A RER ALA TL+D+KTAV KLH M+NI+ +++I+VIWL++L IA+SK L
Sbjct: 642 KSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVL 701

Query: 648 LFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEMNILTTIFL 707
           LF +SQ+VL+AF+FGNT KTVFESIIFLF++HP+DVG+ CEI+ VQ++V+EMNILTT+FL
Sbjct: 702 LFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFL 761

Query: 708 RYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRIISYIEGKK 767
           RYDN KI+ PNSLL   +I+N+YRSPDMGDA+EFC+HI TP EKI++++QRI +YI+ K 
Sbjct: 762 RYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKP 821

Query: 768 KHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKIFQEIDLQY 791
           ++W P  +I +KD+E+L+ +R+AI   HR+NHQDM +RWTRRA+LVEE++KI  E+D+Q+
Sbjct: 822 EYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQH 879

BLAST of HG10000922 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.6e-215
Identity = 433/863 (50.17%), Postives = 572/863 (66.28%), Query Frame = 0

Query: 11  KEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFW-NDSDNRLNNGEVTNGNGGGG 70
           ++ +V++D +D               S K W+ESS +FW ND  ++   GE  +G     
Sbjct: 10  RDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGEEDDG----- 69

Query: 71  RSEDFELRQQRKDVE-DPPSKLIGQLLHKKKGSG-EISLDMDMEMLELPQDKTLLPMVAD 130
            S DF  R   K  E DPPSKLI Q L+K+K SG EISLDM+  M EL Q  T+ P+ + 
Sbjct: 70  -SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNTVPPLSST 129

Query: 131 SPMRRRHRDSLPVDEEHR---GKQPQQRDLRA-------HGSNGEDD----GGAEVLRCT 190
           +        + PV   +R   G   ++R  R         G + ED+     G+EV++CT
Sbjct: 130 AVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCT 189

Query: 191 SNSSFGRDVSFQR------------------------KSGQVRRGLLSKAID-------- 250
           SN S  R  +  +                        +SG +  G   +           
Sbjct: 190 SNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGG 249

Query: 251 ----EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKL 310
               EE++DPF EED+P+  +K  + V  +++W  LILIIA+LIC+L I   R K LW L
Sbjct: 250 SKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDL 309

Query: 311 ELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLV 370
            LWKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYFVYGIRK VQNCLWLGLV
Sbjct: 310 ALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLV 369

Query: 371 LIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDR 430
           LIAW+FLFD KV+RE++S  L+YVTKVL+CLLV+ ++WL+KTL+VKVLAS FH+STYFDR
Sbjct: 370 LIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDR 429

Query: 431 IQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATI----------PPDL 490
           IQ +LF QYVI TL GPP +EI     EEE+VA +V   E  G  +          PP +
Sbjct: 430 IQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQV 489

Query: 491 ---KATAQKGGRVIG----------------EGITIDHLHKLSPKNVSAWNMKRLMKIIR 550
                  QK    +G                EGI IDHL +++ KNVSAW MK+LM +I+
Sbjct: 490 TVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIK 549

Query: 551 HVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMD 610
             TLSTLDEQI+D+ T E++  TQI+S +EAK+AA+KIF NVA  GS+YIY+ED  RF+ 
Sbjct: 550 KGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLS 609

Query: 611 EDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNIL 670
           ED++ + M LFE A E  KISKS LKNWV+ A RER ALA TL+D+KTAV +LHR+V++L
Sbjct: 610 EDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVL 669

Query: 671 ISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNL 730
           +S++IL+IWLLILGIAT+KFLL  +SQL+LV FVFGN+CKT+FE++IF+FVMHPFDVG+ 
Sbjct: 670 VSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDR 729

Query: 731 CEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHIC 790
           CEI+GVQMIV+EMNILTT+FLR+DNQKI+ PNSLL T  I N+YRSPDM DA+EF +HI 
Sbjct: 730 CEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIA 789

Query: 791 TPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRW 792
           TPPEK   +RQRI+SY++ KK HW P+P I  +D+  LN ++IA+  TH+MNHQ+MG+R+
Sbjct: 790 TPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERY 849

BLAST of HG10000922 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 3.1e-206
Identity = 374/621 (60.23%), Postives = 487/621 (78.42%), Query Frame = 0

Query: 188 SGQVRRGLLSKAID--EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLA 247
           S Q+R GLL +  D  EE+DD   EEDVP +Y+K  +  +TLLQW SLI ++ AL+ +L 
Sbjct: 193 SDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLG 252

Query: 248 IRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIR 307
           +  WR   LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYG++
Sbjct: 253 LHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVK 312

Query: 308 KAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVL 367
            AVQNCLWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L+ST++WL+KTL+VKVL
Sbjct: 313 TAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVL 372

Query: 368 ASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPP 427
           AS FHVSTYFDRIQ ALF  Y+I TL GPP++E+S+ EEEE+R  +E+ K++  GA + P
Sbjct: 373 ASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSP 432

Query: 428 DLKATA---QKGGRVIG-------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRH 487
           +L + A   +K G  +               GIT+D LHK++ KNVSAWNMKRLMKI+R+
Sbjct: 433 ELCSAAFPQEKSGSTMNMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRN 492

Query: 488 VTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDE 547
           V+LSTLDEQ   + T E+EST QI+S  EAK AA+KIF NVA+ G+K+IY+EDL RF+  
Sbjct: 493 VSLSTLDEQALQN-TCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRV 552

Query: 548 DKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILI 607
           D+A KTM LFE A  ++KI+KS+LKNW++ A RER ALA TL+D+KTAV KLH M++ L 
Sbjct: 553 DEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLT 612

Query: 608 SVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLC 667
           +++I+VIWL++L IATSK+LLF TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVG+  
Sbjct: 613 AIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRL 672

Query: 668 EINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICT 727
            I+ V+M+V+EMNILTT+FLR DN KI+ PN LL   AIHN+ RSPDMGD V  C+HI T
Sbjct: 673 LIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITT 732

Query: 728 PPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWT 787
           PPEKIA ++QRI SYI+ K ++W P   + +KDVE+LN +RIAI L H++NHQ+MG+R+T
Sbjct: 733 PPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFT 792

Query: 788 RRALLVEELVKIFQEIDLQYR 791
           RRALL+EE++KI  E+D+QYR
Sbjct: 793 RRALLIEEVIKILLELDIQYR 812

BLAST of HG10000922 vs. ExPASy TrEMBL
Match: A0A5D3DK11 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00230 PE=3 SV=1)

HSP 1 Score: 1241.5 bits (3211), Expect = 0.0e+00
Identity = 674/864 (78.01%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. ExPASy TrEMBL
Match: A0A1S3BIW1 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490566 PE=3 SV=1)

HSP 1 Score: 1241.5 bits (3211), Expect = 0.0e+00
Identity = 674/864 (78.01%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. ExPASy TrEMBL
Match: A0A5A7V645 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G002610 PE=3 SV=1)

HSP 1 Score: 1239.6 bits (3206), Expect = 0.0e+00
Identity = 673/864 (77.89%), Postives = 720/864 (83.33%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKV++ D S   SLR   LVN SG+IW+ESS DFWNDSDNR NNGE     GG
Sbjct: 49  SDRKEVIVKVNEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              +EDFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 ARTTEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNSSF RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SK +DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKVLDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLL+STLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEV+KL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTTQIKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD VEFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. ExPASy TrEMBL
Match: A0A0A0KE62 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_6G124170 PE=3 SV=1)

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 672/864 (77.78%), Postives = 718/864 (83.10%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S   +LR   LVN SG IW+ESS DFWNDSDNR N GE     GG
Sbjct: 49  SDRKEVIVKVDEADAS---TLRATDLVNGSGTIWRESSYDFWNDSDNRRNYGE-----GG 108

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
              ++DFE RQ RKDVEDPPSKLIGQ LHK+K SGE+SLDMDMEMLELPQDKT L  VA+
Sbjct: 109 AKTTDDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAE 168

Query: 128 SP-----------------------MRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SP                       MRRRHRDS P+DEEHRG+QP+Q D RAHGSNGEDD
Sbjct: 169 SPMRRSSRELKVSFESISEISENDSMRRRHRDS-PLDEEHRGQQPRQCDRRAHGSNGEDD 228

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEVLRC+SNS F RDVSFQR                            KSGQVR GL+
Sbjct: 229 GAAEVLRCSSNSFFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLI 288

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SKA+DEEDDDPFLEED+PD+YKK NLGVLTLLQWASLILIIAAL+CTL IR WRRKKLWK
Sbjct: 289 SKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWK 348

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LE+WKWEVMILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 349 LEVWKWEVMILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGL 408

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLAS FHVSTYFD
Sbjct: 409 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFD 468

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKA----TAQ 487
           RIQ ALF+QYVI TL GPPL+EI KNEEEEER+AEEVIKL+NAGATIPPDLKA    TAQ
Sbjct: 469 RIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTAQ 528

Query: 488 KGGRVIG-------------------------EGITIDHLHKLSPKNVSAWNMKRLMKII 547
           KGGRVIG                         EGITIDHLHKLSPKNVSAWNMKRLM I+
Sbjct: 529 KGGRVIGSGGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIV 588

Query: 548 RHVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFM 607
           RH TLSTLDEQIKD+  HE+ESTT+IKS YEAKVAAKKIFLNVAR+GSKYIY+EDL RFM
Sbjct: 589 RHGTLSTLDEQIKDT-AHEDESTTEIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFM 648

Query: 608 DEDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNI 667
           ++D+ASKTMGLFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNI
Sbjct: 649 EKDEASKTMGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNI 708

Query: 668 LISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGN 727
           L+SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVG+
Sbjct: 709 LVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGD 768

Query: 728 LCEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHI 787
            CEI+GVQMIV+EMNILTTIFLRYDNQKII PNS+LAT AIHNFYRSPDMGD +EFCLHI
Sbjct: 769 RCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGIEFCLHI 828

Query: 788 CTPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKR 792
            TPPEKIAIMRQRIISYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+R
Sbjct: 829 STPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGER 888

BLAST of HG10000922 vs. ExPASy TrEMBL
Match: A0A6J1FSP8 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111448441 PE=3 SV=1)

HSP 1 Score: 1205.3 bits (3117), Expect = 0.0e+00
Identity = 655/862 (75.99%), Postives = 713/862 (82.71%), Query Frame = 0

Query: 8   SDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTNGNGG 67
           SD KEVIVKVD+ D S NAS R ++ VN+SG+IW+ESS DFWNDSD+R NNGE +NG+GG
Sbjct: 56  SDRKEVIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGG 115

Query: 68  GGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLELPQDKTLLPMVAD 127
            G  EDFE RQQRKDVEDPPSKLIGQ LHK+K SGE+ LDMDMEMLELPQDKT LP VA+
Sbjct: 116 KG--EDFEFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKT-LPTVAE 175

Query: 128 SPM-----------------------RRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDD 187
           SPM                       RRRHRDS PVDEEHR + P  RD R HG NG DD
Sbjct: 176 SPMRRSSKELKVSFESISEISESDSLRRRHRDS-PVDEEHRVQHPPLRDHRPHG-NGHDD 235

Query: 188 GGAEVLRCTSNSSFGRDVSFQR----------------------------KSGQVRRGLL 247
           G AEV+ CTSNSSF R+VSFQR                            KSGQ+R GLL
Sbjct: 236 GAAEVVSCTSNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLL 295

Query: 248 SKAIDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 307
           SKA+DEEDDDPFLEED+PD+YKK NLGVLT+LQWASLILIIAALICTL IR WRRKKLWK
Sbjct: 296 SKALDEEDDDPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWK 355

Query: 308 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 367
           LELWKWEVMILVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYG+RKAVQNCLWLGL
Sbjct: 356 LELWKWEVMILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGL 415

Query: 368 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 427
           VLIAWNFLFDDKVQREVKS+ALEYVTKVL+CLLVSTLVWLVKTL+VKVLAS FHVSTYFD
Sbjct: 416 VLIAWNFLFDDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFD 475

Query: 428 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATA---QK 487
           RIQ AL++QYVI TL GPPL+EI KN+EEE ++AEEVIKL+NAGATIPPDL+A+A    +
Sbjct: 476 RIQDALYNQYVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPE 535

Query: 488 GGRVIG------------------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRH 547
           GGRVIG                        EGITIDHLH+LSPKNVSAWNMKRLM I+RH
Sbjct: 536 GGRVIGSGGLQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRH 595

Query: 548 VTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDE 607
             LSTLDEQIKDS  HE+ESTTQIKS YEAKVAAK+IF NVA  GSKYIY+EDL RFM E
Sbjct: 596 GALSTLDEQIKDS-AHEDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVE 655

Query: 608 DKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILI 667
           D+ASKTM LFE A ESRKISKSSLKNWV+ A RER ALA TL+D+KTAV KLHRMVNIL+
Sbjct: 656 DEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILV 715

Query: 668 SVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLC 727
           SVIILVIWLLILGIATSKFLLF TSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVG+ C
Sbjct: 716 SVIILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRC 775

Query: 728 EINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICT 787
           EI+GVQMIV+EMNILTT+FLR+DNQKII PNS+LAT AIHN+YRSPDMGDAVEFCLHI T
Sbjct: 776 EIDGVQMIVEEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHIST 835

Query: 788 PPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWT 792
           PPEKIAIMRQRI+SYIEGKK+HWCPAP I LKDVEELNR+RIAI LTHRMNHQDMG+RWT
Sbjct: 836 PPEKIAIMRQRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWT 895

BLAST of HG10000922 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 827.8 bits (2137), Expect = 7.5e-240
Identity = 465/850 (54.71%), Postives = 601/850 (70.71%), Query Frame = 0

Query: 4   AVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTN 63
           AV  +D +EVIVK+D ++   N  + G     T GKIW++ S DFW D +  LN G    
Sbjct: 2   AVDAADRREVIVKIDGEN-GNNNGVSG----ETVGKIWRDGSYDFWTDGEGNLNKGHNAA 61

Query: 64  G---------NGGGGRSEDFELRQQRKDVEDPPSKLIGQLLHKKKGSGEISLDMDMEMLE 123
                      G   + E FE R+     EDPP+KLIGQ LHK++ SGEI LDMD+ M E
Sbjct: 62  AVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDE 121

Query: 124 LPQDKTLLPMVADSPMRRRHRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGGAEVLRCTS 183
           L Q + L P V++SP     RD  PV          +RD R++ +N  DDG  EV++C+ 
Sbjct: 122 L-QSRGLTP-VSESPRVSTKRD--PVG---------RRDSRSN-TNNNDDG--EVVKCSG 181

Query: 184 NSS----------------------------FGRDVSFQR--KSGQVRRGLLSKAI---- 243
           N++                               D+   R  KSGQ++ G   K+     
Sbjct: 182 NNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQG 241

Query: 244 DEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKLELW 303
           +EE+DDPF  ED+P++Y+K  L +  +L+W SLILIIA  +CTLAI + R+KKLW+L+LW
Sbjct: 242 EEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLW 301

Query: 304 KWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLVLIA 363
           KWE M+LVLICGRLVS W ++I+VFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVL+A
Sbjct: 302 KWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLA 361

Query: 364 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDRIQA 423
           W+FLFD+KV +   + AL  VTK+ VCLLV  L+WLVKTL+VKVLAS FH+STYFDRIQ 
Sbjct: 362 WHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQE 421

Query: 424 ALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPPDLKATAQK------- 483
           +LF QYVI TL GPPL+EI KNEEEEER++ EV K +N G     ++++ AQK       
Sbjct: 422 SLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGV---EIQSGAQKSPMKTGK 481

Query: 484 ------------GGRVIGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIKD 543
                       GG    +GITID LHKL+PKNVSAW MKRLM IIR+ +L+TLDEQ++D
Sbjct: 482 SPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQD 541

Query: 544 SITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFEE 603
             + +++   QI+S +EAK+AA+KIF NVA+ GSK+IY  D+ RF+ +D+A KT+ LFE 
Sbjct: 542 P-SLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEG 601

Query: 604 ARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLIL 663
           A E+ +ISKSSLKNWV+ A RER ALA TL+D+KTAV +LH+MVNI++ +IILVIWL+IL
Sbjct: 602 ASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIIL 661

Query: 664 GIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKEM 723
           GI ++KFL+  +SQ+V+VAF+FGN CK VFESII+LFV+HPFDVG+ CEI+GVQM+V+EM
Sbjct: 662 GITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEM 721

Query: 724 NILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQRI 783
           NILTT+FLR+DNQK++ PNSLL T +I N+YRSPDMGD +EF +HI TP EKI +++QRI
Sbjct: 722 NILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRI 781

Query: 784 ISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVKI 792
            SYIEGKK HW PAP I  KD+E LN +RIA+  THRMNHQDMG++W RR+ LVEE+ KI
Sbjct: 782 TSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKI 822

BLAST of HG10000922 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 808.1 bits (2086), Expect = 6.1e-234
Identity = 452/851 (53.11%), Postives = 593/851 (69.68%), Query Frame = 0

Query: 4   AVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTN 63
           AV  +D ++ IV ++  + SG     G       G IW+ESS DFW D +   N+ +  +
Sbjct: 3   AVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGDD 62

Query: 64  GNGGGGRSEDFELRQQRK----DVEDPPSKLIGQLLHKKKGSG-EISLDMDMEMLELPQD 123
            +  GG S  F  R +R+    ++ DPPSKLIGQ LHK++ SG EISLD+++ M EL  +
Sbjct: 63  EDEDGG-SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 122

Query: 124 KTLLPMVADSPMRRR----HRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGG-----AEV 183
               P  A +  RR        S PV  + +    ++R  R       D+ G     AEV
Sbjct: 123 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDEAEV 182

Query: 184 LRCTS-------NSSFGR---------------DVSFQRKSGQVRRGLLSKA-------- 243
           L+C S       N +  R               ++   R+SG  + G L K+        
Sbjct: 183 LKCGSKKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGR 242

Query: 244 ---IDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 303
               +EE++DPFL+ED+P+++K+  L     L+W SL+LI+ +L+C+L I N +RK  WK
Sbjct: 243 NGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWK 302

Query: 304 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 363
           L+LWKWEV +LVLICGRLVS W +RIIVF +E+NF  RKRVLYFVYG+RK+VQNCLWLGL
Sbjct: 303 LDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGL 362

Query: 364 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 423
           VL+AW+FLFD KV+RE +S AL YVT+VLVCLLV+ ++WLVKT++VKVLAS FH+STYFD
Sbjct: 363 VLLAWHFLFDKKVERETRSTALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFD 422

Query: 424 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLEN-AGATIPPDLKATAQKGG 483
           RIQ +LF QYVI TL GPPLMEI + EEEE++VAE+V  LE  AGA +PP LKAT +   
Sbjct: 423 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 482

Query: 484 RV---------------IGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIK 543
           +V                GEGI ID L +++ KNVSAWNMKRLM II    +STLD+ ++
Sbjct: 483 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 542

Query: 544 DSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFE 603
           D+ T E+E  T I+S YEAK AA+KIF NV   GS+YIY+ED  RF+ E++A + M LFE
Sbjct: 543 DT-TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 602

Query: 604 EARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLI 663
            A ES KISKS LKNWV+ A RER ALA TL+D+KTAV +LHR++N++I +II++IWLLI
Sbjct: 603 GASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 662

Query: 664 LGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKE 723
           LGIAT++FLL  +SQL+LVAFVFGN+CKT+FE+IIFLFVMHPFDVG+ CEI+GVQ++V+E
Sbjct: 663 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 722

Query: 724 MNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQR 783
           MNILTT+FLRYDNQKII PNS+L T  I N+YRSPDMGDAVEFC+HI TPPEKI  ++QR
Sbjct: 723 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 782

Query: 784 IISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVK 792
           I+SY++ KK +W PAP I    +++LN ++IA+ LTHRMNHQDMG+R+ RR LL+EE+ K
Sbjct: 783 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 842

BLAST of HG10000922 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 750.7 bits (1937), Expect = 1.2e-216
Identity = 433/863 (50.17%), Postives = 572/863 (66.28%), Query Frame = 0

Query: 11  KEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFW-NDSDNRLNNGEVTNGNGGGG 70
           ++ +V++D +D               S K W+ESS +FW ND  ++   GE  +G     
Sbjct: 10  RDFVVRIDGEDN------------GDSEKFWRESSINFWHNDKSSKPPGGEEDDG----- 69

Query: 71  RSEDFELRQQRKDVE-DPPSKLIGQLLHKKKGSG-EISLDMDMEMLELPQDKTLLPMVAD 130
            S DF  R   K  E DPPSKLI Q L+K+K SG EISLDM+  M EL Q  T+ P+ + 
Sbjct: 70  -SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPEL-QKNTVPPLSST 129

Query: 131 SPMRRRHRDSLPVDEEHR---GKQPQQRDLRA-------HGSNGEDD----GGAEVLRCT 190
           +        + PV   +R   G   ++R  R         G + ED+     G+EV++CT
Sbjct: 130 AVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCT 189

Query: 191 SNSSFGRDVSFQR------------------------KSGQVRRGLLSKAID-------- 250
           SN S  R  +  +                        +SG +  G   +           
Sbjct: 190 SNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGG 249

Query: 251 ----EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWKL 310
               EE++DPF EED+P+  +K  + V  +++W  LILIIA+LIC+L I   R K LW L
Sbjct: 250 SKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDL 309

Query: 311 ELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGLV 370
            LWKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+VLYFVYGIRK VQNCLWLGLV
Sbjct: 310 ALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLV 369

Query: 371 LIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFDR 430
           LIAW+FLFD KV+RE++S  L+YVTKVL+CLLV+ ++WL+KTL+VKVLAS FH+STYFDR
Sbjct: 370 LIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDR 429

Query: 431 IQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATI----------PPDL 490
           IQ +LF QYVI TL GPP +EI     EEE+VA +V   E  G  +          PP +
Sbjct: 430 IQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFEIVGRKLSPLGPKAVSSPPQV 489

Query: 491 ---KATAQKGGRVIG----------------EGITIDHLHKLSPKNVSAWNMKRLMKIIR 550
                  QK    +G                EGI IDHL +++ KNVSAW MK+LM +I+
Sbjct: 490 TVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIK 549

Query: 551 HVTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMD 610
             TLSTLDEQI+D+ T E++  TQI+S +EAK+AA+KIF NVA  GS+YIY+ED  RF+ 
Sbjct: 550 KGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLS 609

Query: 611 EDKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNIL 670
           ED++ + M LFE A E  KISKS LKNWV+ A RER ALA TL+D+KTAV +LHR+V++L
Sbjct: 610 EDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRLHRIVDVL 669

Query: 671 ISVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNL 730
           +S++IL+IWLLILGIAT+KFLL  +SQL+LV FVFGN+CKT+FE++IF+FVMHPFDVG+ 
Sbjct: 670 VSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDR 729

Query: 731 CEINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHIC 790
           CEI+GVQMIV+EMNILTT+FLR+DNQKI+ PNSLL T  I N+YRSPDM DA+EF +HI 
Sbjct: 730 CEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAIEFFVHIA 789

Query: 791 TPPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRW 792
           TPPEK   +RQRI+SY++ KK HW P+P I  +D+  LN ++IA+  TH+MNHQ+MG+R+
Sbjct: 790 TPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNHQNMGERY 849

BLAST of HG10000922 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 741.5 bits (1913), Expect = 7.1e-214
Identity = 428/851 (50.29%), Postives = 561/851 (65.92%), Query Frame = 0

Query: 4   AVHKSDWKEVIVKVDQDDMSGNASLRGMKLVNTSGKIWQESSNDFWNDSDNRLNNGEVTN 63
           AV  +D ++ IV ++  + SG     G       G IW+ESS DFW D +   N+ +  +
Sbjct: 3   AVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFW-DGEKGKNDKKGDD 62

Query: 64  GNGGGGRSEDFELRQQRK----DVEDPPSKLIGQLLHKKKGSG-EISLDMDMEMLELPQD 123
            +  GG S  F  R +R+    ++ DPPSKLIGQ LHK++ SG EISLD+++ M EL  +
Sbjct: 63  EDEDGG-SFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 122

Query: 124 KTLLPMVADSPMRRR----HRDSLPVDEEHRGKQPQQRDLRAHGSNGEDDGG-----AEV 183
               P  A +  RR        S PV  + +    ++R  R       D+ G     AEV
Sbjct: 123 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTSLGGSSDEEGRNRDEAEV 182

Query: 184 LRCTS-------NSSFGR---------------DVSFQRKSGQVRRGLLSKA-------- 243
           L+C S       N +  R               ++   R+SG  + G L K+        
Sbjct: 183 LKCGSKKPMLSRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGR 242

Query: 244 ---IDEEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLAIRNWRRKKLWK 303
               +EE++DPFL+ED+P+++K+  L     L+W SL+LI+ +L+C+L I N +RK  WK
Sbjct: 243 NGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWK 302

Query: 304 LELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIRKAVQNCLWLGL 363
           L+LWKWEV +LVLICGRLVS W +RIIVF +E+NF  RKRVLYFVYG+RK+VQNCLWLGL
Sbjct: 303 LDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGL 362

Query: 364 VLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASFFHVSTYFD 423
           VL+AW+FLFD KV+RE +S AL                                  TYFD
Sbjct: 363 VLLAWHFLFDKKVERETRSTALR---------------------------------TYFD 422

Query: 424 RIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLEN-AGATIPPDLKATAQKGG 483
           RIQ +LF QYVI TL GPPLMEI + EEEE++VAE+V  LE  AGA +PP LKAT +   
Sbjct: 423 RIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFM 482

Query: 484 RV---------------IGEGITIDHLHKLSPKNVSAWNMKRLMKIIRHVTLSTLDEQIK 543
           +V                GEGI ID L +++ KNVSAWNMKRLM II    +STLD+ ++
Sbjct: 483 KVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQ 542

Query: 544 DSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDEDKASKTMGLFE 603
           D+ T E+E  T I+S YEAK AA+KIF NV   GS+YIY+ED  RF+ E++A + M LFE
Sbjct: 543 DT-TQEDEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFE 602

Query: 604 EARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILISVIILVIWLLI 663
            A ES KISKS LKNWV  A RER ALA TL+D+KTAV +LHR++N++I +II++IWLLI
Sbjct: 603 GASESDKISKSCLKNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLI 662

Query: 664 LGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLCEINGVQMIVKE 723
           LGIAT++FLL  +SQL+LVAFVFGN+CKT+FE+IIFLFVMHPFDVG+ CEI+GVQ++V+E
Sbjct: 663 LGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEE 722

Query: 724 MNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICTPPEKIAIMRQR 783
           MNILTT+FLRYDNQKII PNS+L T  I N+YRSPDMGDAVEFC+HI TPPEKI  ++QR
Sbjct: 723 MNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQR 782

Query: 784 IISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWTRRALLVEELVK 792
           I+SY++ KK +W PAP I    +++LN ++IA+ LTHRMNHQDMG+R+ RR LL+EE+ K
Sbjct: 783 ILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGK 814

BLAST of HG10000922 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 719.9 bits (1857), Expect = 2.2e-207
Identity = 374/621 (60.23%), Postives = 487/621 (78.42%), Query Frame = 0

Query: 188 SGQVRRGLLSKAID--EEDDDPFLEEDVPDKYKKGNLGVLTLLQWASLILIIAALICTLA 247
           S Q+R GLL +  D  EE+DD   EEDVP +Y+K  +  +TLLQW SLI ++ AL+ +L 
Sbjct: 193 SDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLG 252

Query: 248 IRNWRRKKLWKLELWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGIR 307
           +  WR   LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYG++
Sbjct: 253 LHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVK 312

Query: 308 KAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVL 367
            AVQNCLWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L+ST++WL+KTL+VKVL
Sbjct: 313 TAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVL 372

Query: 368 ASFFHVSTYFDRIQAALFDQYVIGTLLGPPLMEISKNEEEEERVAEEVIKLENAGATIPP 427
           AS FHVSTYFDRIQ ALF  Y+I TL GPP++E+S+ EEEE+R  +E+ K++  GA + P
Sbjct: 373 ASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSP 432

Query: 428 DLKATA---QKGGRVIG-------------EGITIDHLHKLSPKNVSAWNMKRLMKIIRH 487
           +L + A   +K G  +               GIT+D LHK++ KNVSAWNMKRLMKI+R+
Sbjct: 433 ELCSAAFPQEKSGSTMNMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRN 492

Query: 488 VTLSTLDEQIKDSITHENESTTQIKSGYEAKVAAKKIFLNVARHGSKYIYVEDLKRFMDE 547
           V+LSTLDEQ   + T E+EST QI+S  EAK AA+KIF NVA+ G+K+IY+EDL RF+  
Sbjct: 493 VSLSTLDEQALQN-TCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRV 552

Query: 548 DKASKTMGLFEEARESRKISKSSLKNWVIYASRERTALASTLDDSKTAVKKLHRMVNILI 607
           D+A KTM LFE A  ++KI+KS+LKNW++ A RER ALA TL+D+KTAV KLH M++ L 
Sbjct: 553 DEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLT 612

Query: 608 SVIILVIWLLILGIATSKFLLFATSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGNLC 667
           +++I+VIWL++L IATSK+LLF TSQ+VL+AF+FGN+ KTVFESIIFLF++HP+DVG+  
Sbjct: 613 AIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRL 672

Query: 668 EINGVQMIVKEMNILTTIFLRYDNQKIIIPNSLLATMAIHNFYRSPDMGDAVEFCLHICT 727
            I+ V+M+V+EMNILTT+FLR DN KI+ PN LL   AIHN+ RSPDMGD V  C+HI T
Sbjct: 673 LIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITT 732

Query: 728 PPEKIAIMRQRIISYIEGKKKHWCPAPRIFLKDVEELNRIRIAICLTHRMNHQDMGKRWT 787
           PPEKIA ++QRI SYI+ K ++W P   + +KDVE+LN +RIAI L H++NHQ+MG+R+T
Sbjct: 733 PPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFT 792

Query: 788 RRALLVEELVKIFQEIDLQYR 791
           RRALL+EE++KI  E+D+QYR
Sbjct: 793 RRALLIEEVIKILLELDIQYR 812

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900754.10.0e+0079.05mechanosensitive ion channel protein 6-like [Benincasa hispida][more]
XP_008448342.10.0e+0078.01PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo][more]
TYK23963.10.0e+0078.01mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa][more]
KAA0061966.10.0e+0077.89mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa][more]
XP_004140046.10.0e+0077.78mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9SYM11.1e-23854.71Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LH748.6e-23353.11Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
F4IME22.6e-22961.57Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 P... [more]
Q9LPG31.6e-21550.17Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
F4IME13.1e-20660.23Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 P... [more]
Match NameE-valueIdentityDescription
A0A5D3DK110.0e+0078.01Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BIW10.0e+0078.01Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490566 PE=... [more]
A0A5A7V6450.0e+0077.89Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KE620.0e+0077.78Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_6G124170 ... [more]
A0A6J1FSP80.0e+0075.99Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC1114484... [more]
Match NameE-valueIdentityDescription
AT1G78610.17.5e-24054.71mechanosensitive channel of small conductance-like 6 [more]
AT3G14810.16.1e-23453.11mechanosensitive channel of small conductance-like 5 [more]
AT1G53470.11.2e-21650.17mechanosensitive channel of small conductance-like 4 [more]
AT3G14810.27.1e-21450.29mechanosensitive channel of small conductance-like 5 [more]
AT2G17000.12.2e-20760.23Mechanosensitive ion channel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 399..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 123..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..164
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..87
NoneNo IPR availablePANTHERPTHR31618:SF16MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 440..791
coord: 5..128
coord: 160..430
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 4..794
e-value: 6.1E-239
score: 793.6
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 440..791
coord: 160..430
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 5..128
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 642..691
e-value: 3.2E-11
score: 45.0
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 584..784
e-value: 1.8E-21
score: 76.6
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 632..691

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000922.1HG10000922.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity