HG10000732 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000732
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCalcium uniporter protein
LocationChr09: 8627481 .. 8629974 (+)
RNA-Seq ExpressionHG10000732
SyntenyHG10000732
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTCAAGAAAACCCTAGCTCAGCGTCTCTTTAACATATCCAAAATCTCAGCACAATCCCTCACCAAATGCCGGATTTCGTCGTCATCGCTTGCGGTTCGTGTTCCTTCCAATACTAATACTTCCAACATTGCTCATGATCCCGGAGACCATTCGCTGTTTCGTCGTTTTCTTCACAAGAGGGCAGTTTCTCAGCCGGCGATTTCGCCGGAGCTCCGGGAATTGGCGGCCGCAGGGAACATTGCGGAGAAGTTTAAATCTCTAGGGATTGCACCAGATCGTATCCGACTCGATTGCCTTCGTCCCCCTGCATGCGAGACGTTGGCTTCGGATCCGGATAGGTCCGACGTGAAGGATGGTTTGACAGTAGAGGACGCGAGGAAATTGCTCAGGGTAGCGCGGATGGAGATGGTGAAGAGGAAATTGAGAGAAATTCGGAAATCGTGGATTCCGTACTCGGAATTCGTTAGGGTTTGTGATCAGGGATGTGATAATCCTGATCAAGGTCTGGAGATTGCTCAAATGCTTGATGAATCAGGAACTGTGATCGTCTTAGGAAACGTGGTGTACCTCAGGCCTGAACAGGTGATTTGTTGTTTTCCCATCTTGATTTGTGTTTTTATTTTACTTGTCTTCAAATTGCATTAGAGATATTTTTTTTGTTACTTTCTGTCTTTGAGTTCTACAGATTTTGATGCCGCTTACCAAATAGGTTCTGGTTTTCTTTTTTCACGAGATCTTTATTTTAAAGTTCTAATCCTGGATACTACCGAGAACTGATATTCTTTTCTGGTTATTCTTTTTTTTCTTTTGTTTTCTTCAACAGGCCAGATCTAAAACGTTAAAAGTGGTTCCACGTTTGAGATGTGTTATCTGAAGGATCATATAGACGCCCTTCGAGTCATTCAGTGACAAGATTGTTACATTTTCTAGCTACAATGGATATGGAACACCCTGGGTCGGTTTGGTTAGGGGTAGGGGAAGGGGAGGTCTCTGTTATGGCATTTGGGTCTATTAGAATTACCAAAAATTGACCATAATTTCTGATTCAAATGGATTGGGTTTGAAACTCATAGACGAACTTTTTTAGGCAACTTTCTTGGGAACCGGGGAAGGGTATGGTTTATCTTACAGAAAACAATCTTATGAGAAAAATACAAAGAGAGAGGGAGAGAGAAGACGACAAGTTTAGGATGGAAAAGAAGTCATTGAACTTTGTTTTGATCTGAGGGATTCCGGTCGTACATGAACTTAAGTAAGATATACAGGCTTTGTTATATTAGCTGTAGATGAACAAGGTCTAATTTGATCATGGATTGATCATAGTGGACGGAAGGTCTAACCATGCTTTTACTTTCTGGTATATGAGTTTTTTTTGGGATTAATTTTAGGATTCCCTGCCAGAGAAAGTTGGAAGTGTTTTAAGAAGGGACGAATGTTTTTTCGGTAAAAAATAACGAATGTAATTCAATTTATCTAGAAGGATAGGTTTACACCATTCTTGAATCCGTGGCTCTTTTCCTAAAGTTTTCTATATTTCTTTTCCTTCACCTTTTGGGTGGGGCCATTGGATATTGTAACCCCTTTTATTTGTCCCTTCTCTAACTCTAGTCCAAGAAGCTTACTAACTTCCTGATTATATTTTTGAATTACTGACCTAATGTGTTCAAACATTGACAATTTTCATCCTAGAACACATCTAAGCCACCTCGGCCATTTTTTATCCGGACCCCTTTCTAAATTATGAATTCTAATAATGTTTTTATACATACTGTATTTTTTCTAGCTGTGGTGTTTTTGATCCAGTCCCCTTCCTTTCTGAAGTATAATGCTACCAGTTTTTTTTCTAATATATAATACTGCATTTTGATGTGGCTGCCGATCAAGTTTTTACCTTCAAACGGCTTAGTTCATCCAATGAACCCATTAGAACTAACTATAAATATGCTAGGTAGTACAAGTTTTTACCCAAGTATTTCATATTGTTTGACCTCAGGTAGCTAAAGTCATTGAAGGCCTAATTCCCCTACCATCAGTCATTCCAAATGATGGCATGAGGGAAGAACTTGAAGAAATGGAGATGCAGAAAGCTGGTATTGACAAACGAGCCGAAGCACTCACACGACGAGAGCTCTGGTGCGGATTGGGCTTCCTTGTGGCTCAAACAGCTACATTCATGAGGCTCACCTTCTGGGAGCTCACCTGGGATGTCATGGAGCCAATTTGCTTCTATGTCACCTCAGGTTATTTTATTGCTGGCTATAGCTTCTTCCTCAAAACCTCGAAAGAGCCTTCGTTTGAAGGGTTCTTTCAAAGTCGATTTAGCACAAAGCAGAAGCGCCTGATGAAGCTTCAGAATTTTGATATCGCTCGTTACAACGAACTCCGAAGAGCTTGTTTTCCAAATTCCTCAGCTCTAGGACTTCCTTCTTCAGATTCTATGGTGTATGATAATTCAGCAAAGATGCAGTTGGAATCTATACGTCATTAA

mRNA sequence

ATGGCGTTCAAGAAAACCCTAGCTCAGCGTCTCTTTAACATATCCAAAATCTCAGCACAATCCCTCACCAAATGCCGGATTTCGTCGTCATCGCTTGCGGTTCGTGTTCCTTCCAATACTAATACTTCCAACATTGCTCATGATCCCGGAGACCATTCGCTGTTTCGTCGTTTTCTTCACAAGAGGGCAGTTTCTCAGCCGGCGATTTCGCCGGAGCTCCGGGAATTGGCGGCCGCAGGGAACATTGCGGAGAAGTTTAAATCTCTAGGGATTGCACCAGATCGTATCCGACTCGATTGCCTTCGTCCCCCTGCATGCGAGACGTTGGCTTCGGATCCGGATAGGTCCGACGTGAAGGATGGTTTGACAGTAGAGGACGCGAGGAAATTGCTCAGGGTAGCGCGGATGGAGATGGTGAAGAGGAAATTGAGAGAAATTCGGAAATCGTGGATTCCGTACTCGGAATTCGTTAGGGTTTGTGATCAGGGATGTGATAATCCTGATCAAGGTCTGGAGATTGCTCAAATGCTTGATGAATCAGGAACTGTGATCGTCTTAGGAAACGTGGTGTACCTCAGGCCTGAACAGGTAGCTAAAGTCATTGAAGGCCTAATTCCCCTACCATCAGTCATTCCAAATGATGGCATGAGGGAAGAACTTGAAGAAATGGAGATGCAGAAAGCTGGTATTGACAAACGAGCCGAAGCACTCACACGACGAGAGCTCTGGTGCGGATTGGGCTTCCTTGTGGCTCAAACAGCTACATTCATGAGGCTCACCTTCTGGGAGCTCACCTGGGATGTCATGGAGCCAATTTGCTTCTATGTCACCTCAGGTTATTTTATTGCTGGCTATAGCTTCTTCCTCAAAACCTCGAAAGAGCCTTCGTTTGAAGGGTTCTTTCAAAGTCGATTTAGCACAAAGCAGAAGCGCCTGATGAAGCTTCAGAATTTTGATATCGCTCGTTACAACGAACTCCGAAGAGCTTGTTTTCCAAATTCCTCAGCTCTAGGACTTCCTTCTTCAGATTCTATGGTGTATGATAATTCAGCAAAGATGCAGTTGGAATCTATACGTCATTAA

Coding sequence (CDS)

ATGGCGTTCAAGAAAACCCTAGCTCAGCGTCTCTTTAACATATCCAAAATCTCAGCACAATCCCTCACCAAATGCCGGATTTCGTCGTCATCGCTTGCGGTTCGTGTTCCTTCCAATACTAATACTTCCAACATTGCTCATGATCCCGGAGACCATTCGCTGTTTCGTCGTTTTCTTCACAAGAGGGCAGTTTCTCAGCCGGCGATTTCGCCGGAGCTCCGGGAATTGGCGGCCGCAGGGAACATTGCGGAGAAGTTTAAATCTCTAGGGATTGCACCAGATCGTATCCGACTCGATTGCCTTCGTCCCCCTGCATGCGAGACGTTGGCTTCGGATCCGGATAGGTCCGACGTGAAGGATGGTTTGACAGTAGAGGACGCGAGGAAATTGCTCAGGGTAGCGCGGATGGAGATGGTGAAGAGGAAATTGAGAGAAATTCGGAAATCGTGGATTCCGTACTCGGAATTCGTTAGGGTTTGTGATCAGGGATGTGATAATCCTGATCAAGGTCTGGAGATTGCTCAAATGCTTGATGAATCAGGAACTGTGATCGTCTTAGGAAACGTGGTGTACCTCAGGCCTGAACAGGTAGCTAAAGTCATTGAAGGCCTAATTCCCCTACCATCAGTCATTCCAAATGATGGCATGAGGGAAGAACTTGAAGAAATGGAGATGCAGAAAGCTGGTATTGACAAACGAGCCGAAGCACTCACACGACGAGAGCTCTGGTGCGGATTGGGCTTCCTTGTGGCTCAAACAGCTACATTCATGAGGCTCACCTTCTGGGAGCTCACCTGGGATGTCATGGAGCCAATTTGCTTCTATGTCACCTCAGGTTATTTTATTGCTGGCTATAGCTTCTTCCTCAAAACCTCGAAAGAGCCTTCGTTTGAAGGGTTCTTTCAAAGTCGATTTAGCACAAAGCAGAAGCGCCTGATGAAGCTTCAGAATTTTGATATCGCTCGTTACAACGAACTCCGAAGAGCTTGTTTTCCAAATTCCTCAGCTCTAGGACTTCCTTCTTCAGATTCTATGGTGTATGATAATTCAGCAAAGATGCAGTTGGAATCTATACGTCATTAA

Protein sequence

MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLHKRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKDGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDESGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTRRELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEGFFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLPSSDSMVYDNSAKMQLESIRH
Homology
BLAST of HG10000732 vs. NCBI nr
Match: XP_038900870.1 (calcium uniporter protein 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 662.5 bits (1708), Expect = 2.0e-186
Identity = 337/360 (93.61%), Postives = 346/360 (96.11%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVP NTNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPPNTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 120
           KRAVSQPAISPE RELAA GNIAEKFKSLGIAPDRIRLDCLRPPACETL SDPDRSDVKD
Sbjct: 61  KRAVSQPAISPEFRELAAGGNIAEKFKSLGIAPDRIRLDCLRPPACETLVSDPDRSDVKD 120

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GLTVEDARKLLRVARMEMVKR LREI++SWIP+SEFVRVC QGCD+PDQG+EIAQMLDES
Sbjct: 121 GLTVEDARKLLRVARMEMVKRNLREIQESWIPHSEFVRVCSQGCDDPDQGVEIAQMLDES 180

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTRR 240
           GTVIVLGNVVYLRPEQVAK IEGLIPLPSVIPND +REELEE+EMQKA IDKRAEAL RR
Sbjct: 181 GTVIVLGNVVYLRPEQVAKAIEGLIPLPSVIPNDAIREELEELEMQKAAIDKRAEALARR 240

Query: 241 ELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEGF 300
           ELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEGF
Sbjct: 241 ELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEGF 300

Query: 301 FQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLPSSDSMVYDNSAKMQLESIRH 360
           FQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSS  GLP SDSMVYDNSAKMQLESI H
Sbjct: 301 FQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSPHGLP-SDSMVYDNSAKMQLESIHH 359

BLAST of HG10000732 vs. NCBI nr
Match: XP_016902171.1 (PREDICTED: calcium uniporter protein 2, mitochondrial [Cucumis melo] >KAA0045875.1 calcium uniporter protein 2 [Cucumis melo var. makuwa] >TYK13711.1 calcium uniporter protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 632.9 bits (1631), Expect = 1.7e-177
Identity = 327/362 (90.33%), Postives = 339/362 (93.65%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLA RLFN+SKISAQSLTKCRISSSSLAVRVP NTNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAHRLFNLSKISAQSLTKCRISSSSLAVRVPPNTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRAV-SQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVK 120
           KRAV +QPAISPELRELAA GNI EKFKSLGIA DRIRLDCLRPPA ETLA D DRSDVK
Sbjct: 61  KRAVFTQPAISPELRELAAGGNIVEKFKSLGIASDRIRLDCLRPPASETLAVDRDRSDVK 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
           +GLTVEDARKLLRV RMEMVKR LREI+KSWIPY+EF+RVC QGCD+ DQGLEIAQMLDE
Sbjct: 121 NGLTVEDARKLLRVTRMEMVKRNLREIQKSWIPYTEFIRVCCQGCDDSDQGLEIAQMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           SGTVIVLGNVVYLRPEQVAK IEGLIPLPSVIPND +REELE ME+QKA IDKRAEALTR
Sbjct: 181 SGTVIVLGNVVYLRPEQVAKAIEGLIPLPSVIPNDAIREELEAMEIQKAAIDKRAEALTR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELWCGLGFLVAQTA FMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWCGLGFLVAQTAAFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLP-SSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYN+LRRACFPNSSA  LP SSDSMVYDNS KMQLESI
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNKLRRACFPNSSAPELPSSSDSMVYDNSTKMQLESI 360

BLAST of HG10000732 vs. NCBI nr
Match: XP_011649372.1 (calcium uniporter protein 2, mitochondrial [Cucumis sativus] >KGN62066.1 hypothetical protein Csa_006242 [Cucumis sativus])

HSP 1 Score: 620.9 bits (1600), Expect = 6.8e-174
Identity = 319/362 (88.12%), Postives = 336/362 (92.82%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLA RLFN+SKISAQ+LTKCRISSSSLA+R P +TNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAHRLFNLSKISAQALTKCRISSSSLALRFPPHTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRA-VSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVK 120
           KRA V+QPAISPELRELAA GNI EKFKSLGIA DRIRLDCLRPPA ETLA+D DRSDVK
Sbjct: 61  KRAVVTQPAISPELRELAAGGNIVEKFKSLGIASDRIRLDCLRPPASETLATDWDRSDVK 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
           +GLTVEDARKLLRV RMEMVKR LREI+KSW+PYSEFVRVC QGCD+ DQGLEIAQMLDE
Sbjct: 121 NGLTVEDARKLLRVTRMEMVKRDLREIQKSWVPYSEFVRVCCQGCDDSDQGLEIAQMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           SGTVIVLGNVVYLRPEQV K IEGLIPLPSVIPND +R+E E ME+QKA IDKRAE LTR
Sbjct: 181 SGTVIVLGNVVYLRPEQVTKAIEGLIPLPSVIPNDAIRKEFEAMEIQKAAIDKRAETLTR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELWCGLGFLVAQTA FMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWCGLGFLVAQTAAFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLP-SSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYN+LRRACFPNSSA  LP SSDSMVYDNS KMQL+SI
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNKLRRACFPNSSAQELPSSSDSMVYDNSTKMQLDSI 360

BLAST of HG10000732 vs. NCBI nr
Match: XP_022971703.1 (calcium uniporter protein 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 589.0 bits (1517), Expect = 2.8e-164
Identity = 304/361 (84.21%), Postives = 323/361 (89.47%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MA+KKTLAQRLFNISKIS Q LTKCRISSSSLA RVP NTNT+NIAHDPGDH +FR FLH
Sbjct: 1   MAYKKTLAQRLFNISKISEQFLTKCRISSSSLAARVPPNTNTANIAHDPGDHVVFRCFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 120
           K+AVSQPAISPELRELAA GN+ EKFKSLGIAP RIRLDCLRPPAC++LASDPDRSD+K 
Sbjct: 61  KKAVSQPAISPELRELAAGGNMVEKFKSLGIAPHRIRLDCLRPPACDSLASDPDRSDLKG 120

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GLTVEDARKLLRVARMEMVKRKLRE++KSWIP+SEFVR+C QGCD+P QGLE AQMLDES
Sbjct: 121 GLTVEDARKLLRVARMEMVKRKLREVQKSWIPHSEFVRICGQGCDDPGQGLETAQMLDES 180

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPN-DGMREELEEMEMQKAGIDKRAEALTR 240
           GTVIVLGNVVYLRPEQVAK IEGLIPLP VIPN D  REELEEME +KA IDK+AE L R
Sbjct: 181 GTVIVLGNVVYLRPEQVAKAIEGLIPLPPVIPNDDARREELEEMETKKAVIDKKAEMLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELW GLG LVAQTA FMRLTFWELTWDVMEPICFYVTS YFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWVGLGLLVAQTAAFMRLTFWELTWDVMEPICFYVTSAYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLPSSDSMVYDNSAKMQLESIR 360
           FFQSRFSTKQKRLMKLQNFDIARYNEL +AC P SSA GLPSSDS VY      +LES+ 
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNELCKACPPKSSAQGLPSSDSKVY------ELESLH 355

BLAST of HG10000732 vs. NCBI nr
Match: XP_022928069.1 (calcium uniporter protein 2, mitochondrial-like [Cucurbita moschata] >KAG6571403.1 Calcium uniporter protein 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011167.1 Calcium uniporter protein 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 589.0 bits (1517), Expect = 2.8e-164
Identity = 304/362 (83.98%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MA+KKTLAQRLFNISKIS Q LTKCRISSSSLA RVP NTNT+NIAHDPGDH +FRRFLH
Sbjct: 1   MAYKKTLAQRLFNISKISEQFLTKCRISSSSLAARVPPNTNTANIAHDPGDHVVFRRFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 120
           K+AVSQPAISPELRELAA GN+ EKFKSLG+AP RIRLDCLRPPAC++LASDPDRSD+K 
Sbjct: 61  KKAVSQPAISPELRELAAGGNMVEKFKSLGLAPHRIRLDCLRPPACDSLASDPDRSDLKG 120

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GLTVEDARKLL+VARMEMVKRKLRE++KSWIP+SEFVR+C QGCD+PDQGLE AQMLDES
Sbjct: 121 GLTVEDARKLLKVARMEMVKRKLREVQKSWIPHSEFVRICGQGCDDPDQGLETAQMLDES 180

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPN-DGMREELEEMEMQKAGIDKRAEALTR 240
           GTVIVLGNVVYLRPEQVAK IEGLIPLP VIPN D  REELEEME +KA IDK+AE L R
Sbjct: 181 GTVIVLGNVVYLRPEQVAKAIEGLIPLPPVIPNDDARREELEEMETKKAVIDKKAEMLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELW GLG LVAQTA FMRLTFWELTWDVMEPICFYVTS YFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWVGLGLLVAQTAAFMRLTFWELTWDVMEPICFYVTSAYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNEL-RRACFPNSSALGLPSSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYNEL + AC P SSA GLPSSDS VY      +LES+
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNELCKAACPPKSSAQGLPSSDSKVY------ELESL 356

BLAST of HG10000732 vs. ExPASy Swiss-Prot
Match: O64823 (Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g23790 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.1e-93
Identity = 183/332 (55.12%), Postives = 242/332 (72.89%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAV--RVPS-NTNTSNIAHDPGDHSLFRR 60
           MA +K L+++LFNI+ +++QSL  CRISSSSLAV  RVP+ +T+T+ IA +PGD ++ RR
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAPEPGDLAMSRR 60

Query: 61  FLHKRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSD 120
           F+H  A+ +PA   E+ ++    ++ EK + +  + DRIRLD L PP  ET         
Sbjct: 61  FMHNSAMIRPA---EIMQMPVGESLIEKLREIDGSKDRIRLDGLSPPERET--------- 120

Query: 121 VKDGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQML 180
               LTV D +KLLR A++E+VK KLRE  +SW+PY EFV VC +   +PD G +IA+ML
Sbjct: 121 ---SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSDPDLGSKIAKML 180

Query: 181 DESGTVIVLGNVVYLRPEQVAKVIEGLIPLPSV-IPNDGMREELEEMEMQKAGIDKRAEA 240
           D+S  VIVLG+ V +RP+QV K IEGL+PLP +  PND  R E +E+E +KA ID +A  
Sbjct: 181 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 240

Query: 241 LTRRELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPS 300
           L R+ELW GLG+L+ QTA FMRLTFWEL+WDVMEPICFYVTS YF+AGY+FFL+TSKEPS
Sbjct: 241 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 300

Query: 301 FEGFFQSRFSTKQKRLMKLQNFDIARYNELRR 329
           FEGF+QSRF  KQ++LM    FD+ RYNEL++
Sbjct: 301 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKK 317

BLAST of HG10000732 vs. ExPASy Swiss-Prot
Match: Q1PE15 (Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g36820 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 6.1e-85
Identity = 172/336 (51.19%), Postives = 231/336 (68.75%), Query Frame = 0

Query: 4   KKTLAQRLFNISKISAQSLTKCRISSSS---LAVRVPSNTNTSNIAHDPGDHSLFRRFLH 63
           KK L+ RLFN+SK ++QSL  CR SSSS   +  RVP +   + I  +PGD ++ +RFL+
Sbjct: 5   KKLLSNRLFNMSKTASQSLMNCRTSSSSSLAMRTRVPKDIGEATIDPEPGDLTISQRFLN 64

Query: 64  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 123
           K +++         +++   ++ EK K + +  DRIRLD L  P  ETL           
Sbjct: 65  KFSMNG---IDTTSKMSIGESLMEKLKEMDMNKDRIRLDGLSHPKEETL----------- 124

Query: 124 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 183
           GLTV+D +KLLR A +E++K KL E  K WI YS+F+ VC     +P QG  IA+MLD+S
Sbjct: 125 GLTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDPSQGALIAKMLDDS 184

Query: 184 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSV-IPNDGMREELEEMEMQKAGIDKRAEALTR 243
           G VIV+GN V LRP Q+ K IEGL+PL  +  PND  R+EL E+E  K  ID++A +L R
Sbjct: 185 GNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAHSLVR 244

Query: 244 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 303
           RELW GLG+L+ QTA FMRLTFW+LTWDVMEPICFYV+S YF+AGY+FFLKTS+EPSF+G
Sbjct: 245 RELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREPSFQG 304

Query: 304 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSS 336
           F+QSRF  KQ++LM+ ++FD+ RY+EL++   P  S
Sbjct: 305 FYQSRFEAKQRKLMQSEDFDVGRYDELKKLFNPKPS 326

BLAST of HG10000732 vs. ExPASy Swiss-Prot
Match: Q9LVR5 (Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g66650 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.2e-82
Identity = 172/339 (50.74%), Postives = 226/339 (66.67%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           M+ KK+L Q LFNISK      T  RIS  +        T +  I  D GD  + RRFLH
Sbjct: 1   MSSKKSLVQSLFNISK------TYSRISGLTRM----RPTKSGGIPPDAGDSGIRRRFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIA-PDRIRLDCLRPPACETLASDPDRSDVK 120
           KRA   P I P+       GN+ EK + L ++  +RIRLD + PP     +S     +  
Sbjct: 61  KRAFFSPEIVPK------GGNLMEKLRELTLSNNNRIRLDEMLPPPSPKKSS----PEFF 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
             +TVED +KL+R A ME+VK KLREI K+W+PYSEFVRVC +   +P+QG  +A MLDE
Sbjct: 121 PAVTVEDVKKLMRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSSDPEQGNRVANMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           +G VIVLG +V L+PE++   + GLIP      +   R+E E++E+ K+ IDKRA+ L R
Sbjct: 181 AGNVIVLGKLVCLKPEELTSAMAGLIPTLEPSLDAETRQEFEQLEIIKSDIDKRADDLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           +ELW GLG ++AQT  F RLTFWEL+WDVMEPICFYVTS YF+AGY+FFL+TSKEPSFEG
Sbjct: 241 KELWAGLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGYAFFLRTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALG 339
           F++SRF TKQKRL+K+ +FDI R+ +L++   PN +  G
Sbjct: 301 FYKSRFETKQKRLIKMLDFDIDRFTKLQKMHRPNLTKSG 319

BLAST of HG10000732 vs. ExPASy Swiss-Prot
Match: Q9FJV7 (Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g42610 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.6e-61
Identity = 115/206 (55.83%), Postives = 152/206 (73.79%), Query Frame = 0

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GL+V + +KLLR+ +ME VK +LREI KS + Y EF+++C + C N +QG ++A+ LD S
Sbjct: 73  GLSVSETKKLLRLYQMEKVKERLREIPKSSVSYWEFIQICCESCGNDEQGSQMAKSLDHS 132

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTRR 240
           G V+VLG++V+L P Q+AK +E +I   SV+PND  +EEL ++   K  ID  A  + + 
Sbjct: 133 GCVVVLGDIVFLHPHQIAKSMEAMIKQTSVLPNDPRKEELVQLATTKKSIDIEARRIVQA 192

Query: 241 ELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEGF 300
           EL+CGLGFL  QT  FMRLTFWEL+WDVMEPICF+VT+ +FI GY FFL+TS EPSFEG 
Sbjct: 193 ELYCGLGFLAVQTIGFMRLTFWELSWDVMEPICFFVTTIHFILGYIFFLRTSTEPSFEGL 252

Query: 301 FQSRFSTKQKRLMKLQNFDIARYNEL 327
           F+ RF TKQK+LM+   FD  RYNEL
Sbjct: 253 FRQRFKTKQKKLMERHGFDFLRYNEL 278

BLAST of HG10000732 vs. ExPASy Swiss-Prot
Match: F4I111 (Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g09575 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.6e-35
Identity = 81/229 (35.37%), Postives = 135/229 (58.95%), Query Frame = 0

Query: 105 ACETLAS-DPDRSDVKDGLTVEDARKLLRVARMEMVKRKLREIR-KSWIPYSEFVRVCDQ 164
           +C T+ +   D  + K  +T+ +A+KL+R+  +E +K+KL  +  +  + YS  +    Q
Sbjct: 32  SCVTVKTPSEDEEEKKKEITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLE-ASQ 91

Query: 165 G---CDNPDQGLEIAQMLDESGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREE 224
           G     +PD+    A++LD++G V++  + VYL P++V  +I   +PL      D ++EE
Sbjct: 92  GMGIARSPDEAHVFARVLDDAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEE 151

Query: 225 LEEMEMQKAGIDKRAEALTRRELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSG 284
             ++ + K  ID  A    R+ LWCGL   + Q   F RLTFWE +WDVMEPI F+ T+ 
Sbjct: 152 FNKLRIMKEEIDVLAHRQVRKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATAT 211

Query: 285 YFIAGYSFFLKTSKEPSFEGFFQSRFSTKQKRLMKLQNFDIARYNELRR 329
             I GY++FL TS++P+++ F +  F ++Q++L+K   FD  R+ EL R
Sbjct: 212 GIIVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDCERFKELER 259

BLAST of HG10000732 vs. ExPASy TrEMBL
Match: A0A5A7TWU9 (Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002350 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 8.3e-178
Identity = 327/362 (90.33%), Postives = 339/362 (93.65%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLA RLFN+SKISAQSLTKCRISSSSLAVRVP NTNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAHRLFNLSKISAQSLTKCRISSSSLAVRVPPNTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRAV-SQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVK 120
           KRAV +QPAISPELRELAA GNI EKFKSLGIA DRIRLDCLRPPA ETLA D DRSDVK
Sbjct: 61  KRAVFTQPAISPELRELAAGGNIVEKFKSLGIASDRIRLDCLRPPASETLAVDRDRSDVK 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
           +GLTVEDARKLLRV RMEMVKR LREI+KSWIPY+EF+RVC QGCD+ DQGLEIAQMLDE
Sbjct: 121 NGLTVEDARKLLRVTRMEMVKRNLREIQKSWIPYTEFIRVCCQGCDDSDQGLEIAQMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           SGTVIVLGNVVYLRPEQVAK IEGLIPLPSVIPND +REELE ME+QKA IDKRAEALTR
Sbjct: 181 SGTVIVLGNVVYLRPEQVAKAIEGLIPLPSVIPNDAIREELEAMEIQKAAIDKRAEALTR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELWCGLGFLVAQTA FMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWCGLGFLVAQTAAFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLP-SSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYN+LRRACFPNSSA  LP SSDSMVYDNS KMQLESI
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNKLRRACFPNSSAPELPSSSDSMVYDNSTKMQLESI 360

BLAST of HG10000732 vs. ExPASy TrEMBL
Match: A0A1S4E1R8 (Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103497458 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 8.3e-178
Identity = 327/362 (90.33%), Postives = 339/362 (93.65%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLA RLFN+SKISAQSLTKCRISSSSLAVRVP NTNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAHRLFNLSKISAQSLTKCRISSSSLAVRVPPNTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRAV-SQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVK 120
           KRAV +QPAISPELRELAA GNI EKFKSLGIA DRIRLDCLRPPA ETLA D DRSDVK
Sbjct: 61  KRAVFTQPAISPELRELAAGGNIVEKFKSLGIASDRIRLDCLRPPASETLAVDRDRSDVK 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
           +GLTVEDARKLLRV RMEMVKR LREI+KSWIPY+EF+RVC QGCD+ DQGLEIAQMLDE
Sbjct: 121 NGLTVEDARKLLRVTRMEMVKRNLREIQKSWIPYTEFIRVCCQGCDDSDQGLEIAQMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           SGTVIVLGNVVYLRPEQVAK IEGLIPLPSVIPND +REELE ME+QKA IDKRAEALTR
Sbjct: 181 SGTVIVLGNVVYLRPEQVAKAIEGLIPLPSVIPNDAIREELEAMEIQKAAIDKRAEALTR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELWCGLGFLVAQTA FMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWCGLGFLVAQTAAFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLP-SSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYN+LRRACFPNSSA  LP SSDSMVYDNS KMQLESI
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNKLRRACFPNSSAPELPSSSDSMVYDNSTKMQLESI 360

BLAST of HG10000732 vs. ExPASy TrEMBL
Match: A0A0A0LQ26 (Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_2G295420 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.3e-174
Identity = 319/362 (88.12%), Postives = 336/362 (92.82%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MAFKKTLA RLFN+SKISAQ+LTKCRISSSSLA+R P +TNTSNIAHDPGDHS+FRRFLH
Sbjct: 1   MAFKKTLAHRLFNLSKISAQALTKCRISSSSLALRFPPHTNTSNIAHDPGDHSVFRRFLH 60

Query: 61  KRA-VSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVK 120
           KRA V+QPAISPELRELAA GNI EKFKSLGIA DRIRLDCLRPPA ETLA+D DRSDVK
Sbjct: 61  KRAVVTQPAISPELRELAAGGNIVEKFKSLGIASDRIRLDCLRPPASETLATDWDRSDVK 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
           +GLTVEDARKLLRV RMEMVKR LREI+KSW+PYSEFVRVC QGCD+ DQGLEIAQMLDE
Sbjct: 121 NGLTVEDARKLLRVTRMEMVKRDLREIQKSWVPYSEFVRVCCQGCDDSDQGLEIAQMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           SGTVIVLGNVVYLRPEQV K IEGLIPLPSVIPND +R+E E ME+QKA IDKRAE LTR
Sbjct: 181 SGTVIVLGNVVYLRPEQVTKAIEGLIPLPSVIPNDAIRKEFEAMEIQKAAIDKRAETLTR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELWCGLGFLVAQTA FMRLTFWELTWDVMEPICFYVTSGYFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWCGLGFLVAQTAAFMRLTFWELTWDVMEPICFYVTSGYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLP-SSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYN+LRRACFPNSSA  LP SSDSMVYDNS KMQL+SI
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNKLRRACFPNSSAQELPSSSDSMVYDNSTKMQLDSI 360

BLAST of HG10000732 vs. ExPASy TrEMBL
Match: A0A6J1I7N3 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111470372 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.4e-164
Identity = 304/361 (84.21%), Postives = 323/361 (89.47%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MA+KKTLAQRLFNISKIS Q LTKCRISSSSLA RVP NTNT+NIAHDPGDH +FR FLH
Sbjct: 1   MAYKKTLAQRLFNISKISEQFLTKCRISSSSLAARVPPNTNTANIAHDPGDHVVFRCFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 120
           K+AVSQPAISPELRELAA GN+ EKFKSLGIAP RIRLDCLRPPAC++LASDPDRSD+K 
Sbjct: 61  KKAVSQPAISPELRELAAGGNMVEKFKSLGIAPHRIRLDCLRPPACDSLASDPDRSDLKG 120

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GLTVEDARKLLRVARMEMVKRKLRE++KSWIP+SEFVR+C QGCD+P QGLE AQMLDES
Sbjct: 121 GLTVEDARKLLRVARMEMVKRKLREVQKSWIPHSEFVRICGQGCDDPGQGLETAQMLDES 180

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPN-DGMREELEEMEMQKAGIDKRAEALTR 240
           GTVIVLGNVVYLRPEQVAK IEGLIPLP VIPN D  REELEEME +KA IDK+AE L R
Sbjct: 181 GTVIVLGNVVYLRPEQVAKAIEGLIPLPPVIPNDDARREELEEMETKKAVIDKKAEMLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELW GLG LVAQTA FMRLTFWELTWDVMEPICFYVTS YFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWVGLGLLVAQTAAFMRLTFWELTWDVMEPICFYVTSAYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALGLPSSDSMVYDNSAKMQLESIR 360
           FFQSRFSTKQKRLMKLQNFDIARYNEL +AC P SSA GLPSSDS VY      +LES+ 
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNELCKACPPKSSAQGLPSSDSKVY------ELESLH 355

BLAST of HG10000732 vs. ExPASy TrEMBL
Match: A0A6J1EJS9 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111434963 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.4e-164
Identity = 304/362 (83.98%), Postives = 325/362 (89.78%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           MA+KKTLAQRLFNISKIS Q LTKCRISSSSLA RVP NTNT+NIAHDPGDH +FRRFLH
Sbjct: 1   MAYKKTLAQRLFNISKISEQFLTKCRISSSSLAARVPPNTNTANIAHDPGDHVVFRRFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 120
           K+AVSQPAISPELRELAA GN+ EKFKSLG+AP RIRLDCLRPPAC++LASDPDRSD+K 
Sbjct: 61  KKAVSQPAISPELRELAAGGNMVEKFKSLGLAPHRIRLDCLRPPACDSLASDPDRSDLKG 120

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GLTVEDARKLL+VARMEMVKRKLRE++KSWIP+SEFVR+C QGCD+PDQGLE AQMLDES
Sbjct: 121 GLTVEDARKLLKVARMEMVKRKLREVQKSWIPHSEFVRICGQGCDDPDQGLETAQMLDES 180

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPN-DGMREELEEMEMQKAGIDKRAEALTR 240
           GTVIVLGNVVYLRPEQVAK IEGLIPLP VIPN D  REELEEME +KA IDK+AE L R
Sbjct: 181 GTVIVLGNVVYLRPEQVAKAIEGLIPLPPVIPNDDARREELEEMETKKAVIDKKAEMLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           RELW GLG LVAQTA FMRLTFWELTWDVMEPICFYVTS YFIAGY+FFLKTSKEPSFEG
Sbjct: 241 RELWVGLGLLVAQTAAFMRLTFWELTWDVMEPICFYVTSAYFIAGYTFFLKTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNEL-RRACFPNSSALGLPSSDSMVYDNSAKMQLESI 360
           FFQSRFSTKQKRLMKLQNFDIARYNEL + AC P SSA GLPSSDS VY      +LES+
Sbjct: 301 FFQSRFSTKQKRLMKLQNFDIARYNELCKAACPPKSSAQGLPSSDSKVY------ELESL 356

BLAST of HG10000732 vs. TAIR 10
Match: AT2G23790.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 344.0 bits (881), Expect = 1.5e-94
Identity = 183/332 (55.12%), Postives = 242/332 (72.89%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAV--RVPS-NTNTSNIAHDPGDHSLFRR 60
           MA +K L+++LFNI+ +++QSL  CRISSSSLAV  RVP+ +T+T+ IA +PGD ++ RR
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAPEPGDLAMSRR 60

Query: 61  FLHKRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSD 120
           F+H  A+ +PA   E+ ++    ++ EK + +  + DRIRLD L PP  ET         
Sbjct: 61  FMHNSAMIRPA---EIMQMPVGESLIEKLREIDGSKDRIRLDGLSPPERET--------- 120

Query: 121 VKDGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQML 180
               LTV D +KLLR A++E+VK KLRE  +SW+PY EFV VC +   +PD G +IA+ML
Sbjct: 121 ---SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSDPDLGSKIAKML 180

Query: 181 DESGTVIVLGNVVYLRPEQVAKVIEGLIPLPSV-IPNDGMREELEEMEMQKAGIDKRAEA 240
           D+S  VIVLG+ V +RP+QV K IEGL+PLP +  PND  R E +E+E +KA ID +A  
Sbjct: 181 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 240

Query: 241 LTRRELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPS 300
           L R+ELW GLG+L+ QTA FMRLTFWEL+WDVMEPICFYVTS YF+AGY+FFL+TSKEPS
Sbjct: 241 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 300

Query: 301 FEGFFQSRFSTKQKRLMKLQNFDIARYNELRR 329
           FEGF+QSRF  KQ++LM    FD+ RYNEL++
Sbjct: 301 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKK 317

BLAST of HG10000732 vs. TAIR 10
Match: AT4G36820.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 315.8 bits (808), Expect = 4.3e-86
Identity = 172/336 (51.19%), Postives = 231/336 (68.75%), Query Frame = 0

Query: 4   KKTLAQRLFNISKISAQSLTKCRISSSS---LAVRVPSNTNTSNIAHDPGDHSLFRRFLH 63
           KK L+ RLFN+SK ++QSL  CR SSSS   +  RVP +   + I  +PGD ++ +RFL+
Sbjct: 5   KKLLSNRLFNMSKTASQSLMNCRTSSSSSLAMRTRVPKDIGEATIDPEPGDLTISQRFLN 64

Query: 64  KRAVSQPAISPELRELAAAGNIAEKFKSLGIAPDRIRLDCLRPPACETLASDPDRSDVKD 123
           K +++         +++   ++ EK K + +  DRIRLD L  P  ETL           
Sbjct: 65  KFSMNG---IDTTSKMSIGESLMEKLKEMDMNKDRIRLDGLSHPKEETL----------- 124

Query: 124 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 183
           GLTV+D +KLLR A +E++K KL E  K WI YS+F+ VC     +P QG  IA+MLD+S
Sbjct: 125 GLTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDPSQGALIAKMLDDS 184

Query: 184 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSV-IPNDGMREELEEMEMQKAGIDKRAEALTR 243
           G VIV+GN V LRP Q+ K IEGL+PL  +  PND  R+EL E+E  K  ID++A +L R
Sbjct: 185 GNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAHSLVR 244

Query: 244 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 303
           RELW GLG+L+ QTA FMRLTFW+LTWDVMEPICFYV+S YF+AGY+FFLKTS+EPSF+G
Sbjct: 245 RELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREPSFQG 304

Query: 304 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSS 336
           F+QSRF  KQ++LM+ ++FD+ RY+EL++   P  S
Sbjct: 305 FYQSRFEAKQRKLMQSEDFDVGRYDELKKLFNPKPS 326

BLAST of HG10000732 vs. TAIR 10
Match: AT5G66650.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 307.4 bits (786), Expect = 1.5e-83
Identity = 172/339 (50.74%), Postives = 226/339 (66.67%), Query Frame = 0

Query: 1   MAFKKTLAQRLFNISKISAQSLTKCRISSSSLAVRVPSNTNTSNIAHDPGDHSLFRRFLH 60
           M+ KK+L Q LFNISK      T  RIS  +        T +  I  D GD  + RRFLH
Sbjct: 1   MSSKKSLVQSLFNISK------TYSRISGLTRM----RPTKSGGIPPDAGDSGIRRRFLH 60

Query: 61  KRAVSQPAISPELRELAAAGNIAEKFKSLGIA-PDRIRLDCLRPPACETLASDPDRSDVK 120
           KRA   P I P+       GN+ EK + L ++  +RIRLD + PP     +S     +  
Sbjct: 61  KRAFFSPEIVPK------GGNLMEKLRELTLSNNNRIRLDEMLPPPSPKKSS----PEFF 120

Query: 121 DGLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDE 180
             +TVED +KL+R A ME+VK KLREI K+W+PYSEFVRVC +   +P+QG  +A MLDE
Sbjct: 121 PAVTVEDVKKLMRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSSDPEQGNRVANMLDE 180

Query: 181 SGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTR 240
           +G VIVLG +V L+PE++   + GLIP      +   R+E E++E+ K+ IDKRA+ L R
Sbjct: 181 AGNVIVLGKLVCLKPEELTSAMAGLIPTLEPSLDAETRQEFEQLEIIKSDIDKRADDLVR 240

Query: 241 RELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEG 300
           +ELW GLG ++AQT  F RLTFWEL+WDVMEPICFYVTS YF+AGY+FFL+TSKEPSFEG
Sbjct: 241 KELWAGLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGYAFFLRTSKEPSFEG 300

Query: 301 FFQSRFSTKQKRLMKLQNFDIARYNELRRACFPNSSALG 339
           F++SRF TKQKRL+K+ +FDI R+ +L++   PN +  G
Sbjct: 301 FYKSRFETKQKRLIKMLDFDIDRFTKLQKMHRPNLTKSG 319

BLAST of HG10000732 vs. TAIR 10
Match: AT5G42610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 115/206 (55.83%), Postives = 152/206 (73.79%), Query Frame = 0

Query: 121 GLTVEDARKLLRVARMEMVKRKLREIRKSWIPYSEFVRVCDQGCDNPDQGLEIAQMLDES 180
           GL+V + +KLLR+ +ME VK +LREI KS + Y EF+++C + C N +QG ++A+ LD S
Sbjct: 73  GLSVSETKKLLRLYQMEKVKERLREIPKSSVSYWEFIQICCESCGNDEQGSQMAKSLDHS 132

Query: 181 GTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREELEEMEMQKAGIDKRAEALTRR 240
           G V+VLG++V+L P Q+AK +E +I   SV+PND  +EEL ++   K  ID  A  + + 
Sbjct: 133 GCVVVLGDIVFLHPHQIAKSMEAMIKQTSVLPNDPRKEELVQLATTKKSIDIEARRIVQA 192

Query: 241 ELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSGYFIAGYSFFLKTSKEPSFEGF 300
           EL+CGLGFL  QT  FMRLTFWEL+WDVMEPICF+VT+ +FI GY FFL+TS EPSFEG 
Sbjct: 193 ELYCGLGFLAVQTIGFMRLTFWELSWDVMEPICFFVTTIHFILGYIFFLRTSTEPSFEGL 252

Query: 301 FQSRFSTKQKRLMKLQNFDIARYNEL 327
           F+ RF TKQK+LM+   FD  RYNEL
Sbjct: 253 FRQRFKTKQKKLMERHGFDFLRYNEL 278

BLAST of HG10000732 vs. TAIR 10
Match: AT1G09575.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-36
Identity = 81/229 (35.37%), Postives = 135/229 (58.95%), Query Frame = 0

Query: 105 ACETLAS-DPDRSDVKDGLTVEDARKLLRVARMEMVKRKLREIR-KSWIPYSEFVRVCDQ 164
           +C T+ +   D  + K  +T+ +A+KL+R+  +E +K+KL  +  +  + YS  +    Q
Sbjct: 32  SCVTVKTPSEDEEEKKKEITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLE-ASQ 91

Query: 165 G---CDNPDQGLEIAQMLDESGTVIVLGNVVYLRPEQVAKVIEGLIPLPSVIPNDGMREE 224
           G     +PD+    A++LD++G V++  + VYL P++V  +I   +PL      D ++EE
Sbjct: 92  GMGIARSPDEAHVFARVLDDAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEE 151

Query: 225 LEEMEMQKAGIDKRAEALTRRELWCGLGFLVAQTATFMRLTFWELTWDVMEPICFYVTSG 284
             ++ + K  ID  A    R+ LWCGL   + Q   F RLTFWE +WDVMEPI F+ T+ 
Sbjct: 152 FNKLRIMKEEIDVLAHRQVRKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATAT 211

Query: 285 YFIAGYSFFLKTSKEPSFEGFFQSRFSTKQKRLMKLQNFDIARYNELRR 329
             I GY++FL TS++P+++ F +  F ++Q++L+K   FD  R+ EL R
Sbjct: 212 GIIVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDCERFKELER 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900870.12.0e-18693.61calcium uniporter protein 2, mitochondrial-like [Benincasa hispida][more]
XP_016902171.11.7e-17790.33PREDICTED: calcium uniporter protein 2, mitochondrial [Cucumis melo] >KAA0045875... [more]
XP_011649372.16.8e-17488.12calcium uniporter protein 2, mitochondrial [Cucumis sativus] >KGN62066.1 hypothe... [more]
XP_022971703.12.8e-16484.21calcium uniporter protein 2, mitochondrial-like [Cucurbita maxima][more]
XP_022928069.12.8e-16483.98calcium uniporter protein 2, mitochondrial-like [Cucurbita moschata] >KAG6571403... [more]
Match NameE-valueIdentityDescription
O648232.1e-9355.12Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q1PE156.1e-8551.19Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LVR52.2e-8250.74Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9FJV71.6e-6155.83Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
F4I1112.6e-3535.37Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A5A7TWU98.3e-17890.33Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S4E1R88.3e-17890.33Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103497458 PE=3 SV=1[more]
A0A0A0LQ263.3e-17488.12Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_2G295420 PE=3 SV=1[more]
A0A6J1I7N31.4e-16484.21Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111470372 PE=3 SV=1[more]
A0A6J1EJS91.4e-16483.98Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111434963 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G23790.11.5e-9455.12Protein of unknown function (DUF607) [more]
AT4G36820.14.3e-8651.19Protein of unknown function (DUF607) [more]
AT5G66650.11.5e-8350.74Protein of unknown function (DUF607) [more]
AT5G42610.11.1e-6255.83Protein of unknown function (DUF607) [more]
AT1G09575.11.8e-3635.37Protein of unknown function (DUF607) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 213..240
NoneNo IPR availablePANTHERPTHR13462:SF31CALCIUM UNIPORTER PROTEIN 1, MITOCHONDRIALcoord: 1..334
IPR006769Calcium uniporter protein, C-terminalPFAMPF04678MCUcoord: 168..326
e-value: 1.1E-51
score: 175.4
IPR039055MCU familyPANTHERPTHR13462CALCIUM UNIPORTER PROTEIN, MITOCHONDRIALcoord: 1..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000732.1HG10000732.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0015292 uniporter activity