HG10000675 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000675
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionL-ascorbate oxidase homolog
LocationChr09: 7931251 .. 7937944 (-)
RNA-Seq ExpressionHG10000675
SyntenyHG10000675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGAGGGCAATTTGGTAAATTTGATAAATTTGATATTGGGTATCATATTGGTTTGTTGTAGTTTTGGTGGTTGTTGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTCCAAGTCACGTACGGTACACGTGCTCCTCTTGGTGTTAAGCAAAAGGTTATTCCTTTCTTAATCTTCTTCTAATTTACAATTTCAACTCCATTTTATAATATATTTTTTTATAATTTTATATTTTTGAAAGTCGAGCTTATAAATACTAATTTTAAATATTGAAATTTTGAAACCACTTTTAAAAGTGGATAGAAATAATAATAATAACAACGTCTAAAAAATTATAGTGTTTACCAACTTAAATTTCAAGAACAACAAAATAAAAAAATGTAGGTTTATTTTATTTTTTATTTTATTTTATGCTACACAAATGTTGTGAAACTCTAGTTACTTTTCTTTTTTTTTTTAAAAAAAAAATAGAAAATTAAGGAAAACTATTTATACAAAATAGCAAAATATTTAGATAGTTGTGATAGACGTTGATAGAAGTCTATCAGGGTCTATCAATAATAGAAATGATAGAAGTCTATCCTTAATAGATGCTGATAGAAGTTTATCAATGTCTATCAGTATTTTTTTGTTATTTTTTGTAAATAGTTTGACATTTTTTCTATCTGTGAAAATTTTCCTATAATATAAGCTTGTTAACTAATAATTTTGTTTAGGTTAAGTTTTCCATTCTAATTTTTAATATTTTGAGTTTAGTTTCATTTTGTTTTCCTAACATAATTTTTTTTTTAATTAATTAAAATTTTTATGTGACGTAGTATTCCAACGAAAATCGATAATATGTTTTTCAATTACGAAAGAAGTGTTGTTCATGAAAGGAGTAATTTGGTTAGACATGCGTGTTCAACATCCCAATTCCCTTGAGTTCACAATTTTTTTGCTCAACTCATTTCTTTTTTACTTGAAATTTCATTTGATTTTCTTTATTTTCTACCTAACTTAAATCCTAATACAATAAATTAATCAACTATGGATGGTAGCCATTTTTAAATATGATTAGTTTATTACTAAAAAAAAAACCTTAAACCTTAAACCTTAAATTCTACTACATCTAAGAAAACGTGTGTTTTAGGAGAGAAAAGTATTTTACCATCTAATAATAATAATAATGTCATTTGGAAAGATTTTAACCAAATGACTAAAGTGAAAATAAGTACAAAATATAGGTAAATAATAATTTAACCTATATAATTTTGTTTATTAGTTGAACACAAGTTCCAAAGATCCTAAGTTAAAATTTAAAGAGAAAGCAACTTCCAGAACTGCATTTTCTTTTTAGATTTTAAAAGTATTTTTAAAATATAGTTTTTTTAAGTATTATTTATGAAAATACATAACACTAGAGATAAAGCTATTACCAAATAAGACTGGAGGATAATTATTAATACTCTAGTATTGAAAATTGACAAAGACTTGAAGAAAAAAAAATTTGAAAAATGACATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAATAAAATAAAATAAAAAAACTCATTTTGTAATATTGGTATATTGTGTCGTGCAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATTGAGTGTGCGACCAATGACAACATAATTGTAAATGTCATTAACAAGCTTGATGAGCCTTTCCTCTTTACATGGTTAGTCATCAAATTTAATTATTTTTTTATTTTTTATAAAAATTTTAGTGTATGTAATTTCAAATATCGAAATTTTAGTCCATATTTTTAATAAATTTCAAAATTTAGATCATATTGCTAGGTTTGGTTTAACTTTTTTTTTTTTGAAAAAAAAATCAGATTTCCTTTTGTAGATTTTGGACACATTTGCACGTTGGTGATTGGAAATTATTATTATTATTATTTTGCCAAGTTCGATAACAATTAATTTCAAATTAAAAAATCATCATGGGTTGACCTAGTGGTAAAAAAAAGGAGACATAGTCTCGATCAATGGCTAAGAGGTCATAGGTTCAATCCATGATAACAACCTACTTAGAAATTAATATCATTGTCCCGTGAGATTAATCAAGGTACGCGTAAGCTAACCCGAACACTCACATATATAAAAAAAATCAAACTAAAAATATTGACTAAAATAAGATTTTTATGGAAAGTACATCTAAATATAGAAGAAGTTCCATAGTATACAGGGACCAAATTGTATTTTCACCTTATTTATAATACTAAAATTTACACAAGTTTTGGTGAAAGAATGAACCATCTAATGGCCATTTAATTCATAAAATTTAAAGTTTGTGTCTTTTTGGTCTTTGAACTTTCAAAATTACAAATTTTCGTCTCTAATTTTTAGATTTCTTAATATTTATAGATCTAATATACACAAAATTGAAAGTTGAGAAACCTATTAAACAGTTTTAAAAGCTTATGGATTAAATAGACACAATTCTAAAAGTTGAAAGGCTAAATTTGTAATTTTACCCTTGAAAAATATATTAAATTGATTAAGCATTATGAAAGTGTATATATATAAATATAAATATTTTATTCTATTGGAATGTGAATGTGAATAAATTTAGGAGTGGAATCAAGCAAAGGAGGACATCATGGCAAGATGGGGTTTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACCTTCACATACTTCCCTTCCATCAACTTTCAAAGGGCTGCTGGTGCCTTTGGAGCCTTTGTGATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCCGCCGAGCTCGACCTTCTCATCGGCGACTGGTACAACACTGACCATAAGGTAATTTCTTTCTCATTCTACATTCTTTCTACTCTCTCGAATTACTATATTCTTTAACTAGACTTATAAATGTGGAGATCAAAATTCAAATCTTTACGTAATTTTAAAAAAAGTTATTGCGATAAGTAGTTGAATTACGTTTGCGTTTGGGTTCACATTTTTTAAATTGTGAATCTAAGCTACTAATTGGTATGCCATTTTATGTAGACGTTGCGTAAGAAATTGGATTCGGGAATTTCGCTCCCTCTACCTGATGGTCTTCTTATCAATGGACAACCTAGTACAACACTAAGTCTTAAAAAAGGTAACATTCACTCATTATATATAGTTTCTAACATAATTGTAAATATAAAGTCTTATCTAAATATAACATTTTAAAAAAAGTAATTACTAACTATAATATTATTATGGAATAGGTTTTGGGTCTAGTTTCTATTGGTCTGCAGGTTTTCAAAATACCACACTTTTAGTCCTTAAGTTTTGAGTTTGATTTCAATTTAATTCTTAAGTTTCAAAATTTACAATTTTGCCATTGAGATTTGAGTTTTGTTTCGATTTTGCACCCAAATTTCAAGATTTACATTTTTAACCTCAATTTTTCACTAAATACTCACTTCAATCTTTGGTGAAAATGTATATTATTTAATTTAAAATAATTATACAGTGAAATTTTAAAATTAATTTTGACAATGATGAAAAATAGTGAAACTTAATTAATTATAATTTTTTAATTCTTTTCAATGAATTTATAGACATTAACACTAGAGACAGACAGAGAGCATTTAATGAAAAATCGATGTTCAAAACGTAAATCTTAAAACTTAGGGACCAAATTGAAACAAAATTCAAAATTCCAATGGTAAAATTACAACATTTTGAAACTTAGGAAACTAAATTGAAATCTAACTCAAAACCTAGGGACTAAAAAGGTATTTCTCCCATAATCTTCTAAATAATTCATTAACTACTGATGACAATCAATTGCACGTCTCAATTTATACTAGAAACCACACATCACTCGAGAAGACTAATAAAGTAAAAACGCTAAAGTAAAAGGACTAATGAAAAATTTATGTCATGAGTGTTAATAATTTTTATTTTTTTGTAATTTTTGTAGGGCAAACATACAAGTTGAGGATATCAAATGTTGGAATAGCAACATCAATAAACTTTAGAATTCAAGGACATTTGATGACACTTGTGGAGGTAGAAGGAGCTCATACAGTGCAAGAGGCTTATGAGTCATTGGACATTCATCCAGGCCAATCCGCTGCCGTTTTGGTGTCTCTTAACAACTTACCAAAGGATTATTACTTTGTGGCTTCTTCTCGTTTCACTAAACCTATTCTCACTACCATTGGTATCCTTAGATATGAAGGTTCTTCCATCCGTCCTTCCAAGCCTTTGCCCATTGGACCCACTTACCAACTCCATTGGTCCATGAAGCAGGCCCGAACCATCAGGTTCGCTCTATAAACTTGAAAGTCTCATTTCATAACCGGTTCGTTTTGAAGATTCTACTTGTTTTTCTATGATTTCTAAATTACTATATGTTTTCATCTTTTTTAGTTTCAAAACTTTGACTTGGTTTTAAAAACACTCTTAAAAAGTAACATAATAGAAAAATCAATAGCTCAACACAAAATAGTGCTTATGAGCTTAAATTTCAAAACGGGACCTTCATTTTTCAAAATCAAGTTTTTTTCTTTTATTAAGTAATATGTTAGTATCAATATCACGTTGTCTTTATTGTATTGAAACTATTACACATTCGAAAGTTAATATACAAAACTATTCTCTTAATGAAAAAGTCAAAGGTAATATTGTAATTTTGAATAATAATAGTATTTTCAAACAAAAGAGCAAAATTTAGAAATATTTTTTTGTAATGGCAAATCACAAGAAAGGAAAAAAAATACCCGAAAAAAAATACAAAATTACTGTTAACATTTAATAAGTGTTTCAAATTTATAATATTTCAAACCTAACTGCTCCTTTAGATAGATAGTTCTTGGAACTTTGAATGAAAATCAAGAGATAGGAATGGTGTGGCAAAGACTAGAATAGAAATTTGGATCACTGGATCATTCTAAATCACTAACACACATCGTTCTTAGATCGGTAAGGAAAAAGTGCATTGGAGTGTCTACGTAATATCTTAATTAGTTGAGTTGTGCATTTGGAGTATTTACATAATGCCCTATTCTTTTTTAGTAAGAAACTCAATTTTAAATGAAAATTAGGATATAGGAACGACGTCACAGACAGAGACCTAGAATAGAAATTTGAATCACTAGACCATTCTAAGCGACTAACACACACCATTCTTAGATCCATAAGTAAAACACGTGTTAGAGTGATTATATATCCACCGTATACTTATATAATGTCCTAACTAGTTGTGCTATGCTCGTATAGGTAGTAATAGAGTAGCTAGATCTCTATCCAAAAATATATATTACTTATAGAATATCATGACTTAATGATGATATTGTCACACGGATTTTGTTTACAGATTAAATTTGACAGCAAATGCAGCAAGGCCAAACCCACAAGGATCATTCCATTATGGGAACATAACAGTAGTGAGAAGACTGATATTAGAAAATTCATTGAGAAAGATAAAAGGGGCTGTTCGTTGTGCAGTGAACAATGTGTCTTATGTTGATCCACCAACCCCTTTAAAGTTGGCTGATTGGTTCAATATTGGTGGTGTTTTTGACTTCAAAACCTTCACTGATATCCCAACTCCTAGACCTGCAATGCTTGGAGTCTCTGTTGTTAACATTACTCTTCATGATTTTGTGGAAATTGTGTTCCAAAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACCAGTTTCTATGTTGTTGGGTTAGCACTCCTTTTCATAACCCCCTTTTTTCTTTTCTAACATTCTTATTGGCTTTCTTACACTAAATTTTATTAATTTTTATTCTGTTTGATCAGATTTGGTTCTGGTGTTTGGACTTATGACATGAGAAAGCGTTATAATTTAGTGGATGGTATATCAAGACACACCGTTCAGGTACTTTTGTTACTTTCTTCTGTTGTCTGTGTCATTTTCATCGTTGTATATTTATCTACATTACAAGAATATAAGATTTTTTCGATGCAAAAAAAAGTTAAGAAGTTATCAGCTAAAGCTCTCCCGATGCAAAATAAGTTGTCGGGAAGAACTTTTTCTCGATGCTACTAACATGGTGCCGGAAATTGGCCACTTTATCCGACACCACTTTAAAATAAAGTCGGCCAAGGGTCGAGAAATTGGAAAATTGTCCCACTTTCCAATGCTACTAGTATGGCATTGGAAAAAGGGGCTCAATCCGACACCTTTAAAATGATATCAGAAAACATTGTAACAAAAATTCTTGTAGTATCTATACCCTTTGATTAATTGATAGTGATTAATTGATAGTTTCTTTATTAACCTCCTACTTACTTTTATAGAAGAACTTATTATTTATGTTTAATCTTATTGTCATTTGATCTATGATCATTTTGGCCTCATCTCTTATATATTATCGTATATAATAGTGTAATTGGGTGTATTTTTGGGCAGGTATATCCAAAGTCTTGGACTGCTATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTGAGGTCTACAATATGGTGGAGAAACTATTTGGGTCAACAGTTATATTTCAGAGTTTGGAATGATGAAAAGAGCTTATACACTGAGGCTGACATTCCACTCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

mRNA sequence

ATGAGGGAGGGCAATTTGGTAAATTTGATAAATTTGATATTGGGTATCATATTGGTTTGTTGTAGTTTTGGTGGTTGTTGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTCCAAGTCACGTACGGTACACGTGCTCCTCTTGGTGTTAAGCAAAAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATTGAGTGTGCGACCAATGACAACATAATTGTAAATGTCATTAACAAGCTTGATGAGCCTTTCCTCTTTACATGGAGTGGAATCAAGCAAAGGAGGACATCATGGCAAGATGGGGTTTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACCTTCACATACTTCCCTTCCATCAACTTTCAAAGGGCTGCTGGTGCCTTTGGAGCCTTTGTGATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCCGCCGAGCTCGACCTTCTCATCGGCGACTGGTACAACACTGACCATAAGACGTTGCGTAAGAAATTGGATTCGGGAATTTCGCTCCCTCTACCTGATGGTCTTCTTATCAATGGACAACCTAGTACAACACTAAGTCTTAAAAAAGGGCAAACATACAAGTTGAGGATATCAAATGTTGGAATAGCAACATCAATAAACTTTAGAATTCAAGGACATTTGATGACACTTGTGGAGGTAGAAGGAGCTCATACAGTGCAAGAGGCTTATGAGTCATTGGACATTCATCCAGGCCAATCCGCTGCCGTTTTGGTGTCTCTTAACAACTTACCAAAGGATTATTACTTTGTGGCTTCTTCTCGTTTCACTAAACCTATTCTCACTACCATTGGTATCCTTAGATATGAAGGTTCTTCCATCCGTCCTTCCAAGCCTTTGCCCATTGGACCCACTTACCAACTCCATTGGTCCATGAAGCAGGCCCGAACCATCAGATTAAATTTGACAGCAAATGCAGCAAGGCCAAACCCACAAGGATCATTCCATTATGGGAACATAACAGTAGTGAGAAGACTGATATTAGAAAATTCATTGAGAAAGATAAAAGGGGCTGTTCGTTGTGCAGTGAACAATGTGTCTTATGTTGATCCACCAACCCCTTTAAAGTTGGCTGATTGGTTCAATATTGGTGGTGTTTTTGACTTCAAAACCTTCACTGATATCCCAACTCCTAGACCTGCAATGCTTGGAGTCTCTGTTGTTAACATTACTCTTCATGATTTTGTGGAAATTGTGTTCCAAAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACCAGTTTCTATGTTGTTGGATTTGGTTCTGGTGTTTGGACTTATGACATGAGAAAGCGTTATAATTTAGTGGATGGTATATCAAGACACACCGTTCAGGTATATCCAAAGTCTTGGACTGCTATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTGAGGTCTACAATATGGTGGAGAAACTATTTGGGTCAACAGTTATATTTCAGAGTTTGGAATGATGAAAAGAGCTTATACACTGAGGCTGACATTCCACTCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

Coding sequence (CDS)

ATGAGGGAGGGCAATTTGGTAAATTTGATAAATTTGATATTGGGTATCATATTGGTTTGTTGTAGTTTTGGTGGTTGTTGGGTGAATGCTGAAGACCCATATAGATATTTCACTTTCCAAGTCACGTACGGTACACGTGCTCCTCTTGGTGTTAAGCAAAAGGTAATCCTTGTGAATGGGCAATTTCCAGCCCCAAAGATTGAGTGTGCGACCAATGACAACATAATTGTAAATGTCATTAACAAGCTTGATGAGCCTTTCCTCTTTACATGGAGTGGAATCAAGCAAAGGAGGACATCATGGCAAGATGGGGTTTTAGGGACAAATTGCCCAATCCCTCCAAACTCAAATTGGACATACAAATTCCAAGTTAAAGATCAAATTGGAACCTTCACATACTTCCCTTCCATCAACTTTCAAAGGGCTGCTGGTGCCTTTGGAGCCTTTGTGATTCAACCTAGAAGTGTCATAACAACTCCCTACCCCATTCCCGCCGCCGAGCTCGACCTTCTCATCGGCGACTGGTACAACACTGACCATAAGACGTTGCGTAAGAAATTGGATTCGGGAATTTCGCTCCCTCTACCTGATGGTCTTCTTATCAATGGACAACCTAGTACAACACTAAGTCTTAAAAAAGGGCAAACATACAAGTTGAGGATATCAAATGTTGGAATAGCAACATCAATAAACTTTAGAATTCAAGGACATTTGATGACACTTGTGGAGGTAGAAGGAGCTCATACAGTGCAAGAGGCTTATGAGTCATTGGACATTCATCCAGGCCAATCCGCTGCCGTTTTGGTGTCTCTTAACAACTTACCAAAGGATTATTACTTTGTGGCTTCTTCTCGTTTCACTAAACCTATTCTCACTACCATTGGTATCCTTAGATATGAAGGTTCTTCCATCCGTCCTTCCAAGCCTTTGCCCATTGGACCCACTTACCAACTCCATTGGTCCATGAAGCAGGCCCGAACCATCAGATTAAATTTGACAGCAAATGCAGCAAGGCCAAACCCACAAGGATCATTCCATTATGGGAACATAACAGTAGTGAGAAGACTGATATTAGAAAATTCATTGAGAAAGATAAAAGGGGCTGTTCGTTGTGCAGTGAACAATGTGTCTTATGTTGATCCACCAACCCCTTTAAAGTTGGCTGATTGGTTCAATATTGGTGGTGTTTTTGACTTCAAAACCTTCACTGATATCCCAACTCCTAGACCTGCAATGCTTGGAGTCTCTGTTGTTAACATTACTCTTCATGATTTTGTGGAAATTGTGTTCCAAAACAATGAAAACACTGTCCAAGCTTGGCATTTTGATGGAACCAGTTTCTATGTTGTTGGATTTGGTTCTGGTGTTTGGACTTATGACATGAGAAAGCGTTATAATTTAGTGGATGGTATATCAAGACACACCGTTCAGGTATATCCAAAGTCTTGGACTGCTATATTAGTGTCATTAGACAACAAGGGGATGTGGAACTTGAGGTCTACAATATGGTGGAGAAACTATTTGGGTCAACAGTTATATTTCAGAGTTTGGAATGATGAAAAGAGCTTATACACTGAGGCTGACATTCCACTCAATGCTCTCAAGTGTGGCAAAGCATCCAAGTATTAA

Protein sequence

MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKASKY
Homology
BLAST of HG10000675 vs. NCBI nr
Match: XP_038902225.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.4e-308
Identity = 513/542 (94.65%), Postives = 519/542 (95.76%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           M+EGNLV   NL LGIIL+C   GG WVN EDPYRY+TFQVTYGTRAPLGVKQKVILVNG
Sbjct: 1   MKEGNLV---NLTLGIILICYGLGGGWVNGEDPYRYYTFQVTYGTRAPLGVKQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIEC TNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRAAG FGAFVIQPRSVI TPYPIPAAELDLLIGDWY+TDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRSVIATPYPIPAAELDLLIGDWYDTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKLDSGI LPLP+ LLINGQPSTTL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLDSGIMLPLPNRLLINGQPSTTLILKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE 300
           LVEVEG HTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE
Sbjct: 241 LVEVEGGHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE 300

Query: 301 GSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN 360
           GSSI PSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN
Sbjct: 301 GSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN 360

Query: 361 SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420
           SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTD PT RPAMLGVSVVNI
Sbjct: 361 SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDFPTSRPAMLGVSVVNI 420

Query: 421 TLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYP 480
           TLHDFVEIVFQNNENTVQAWHFDGTSFYVVG+GSGVW YDMRKRYNLVDGISRHTVQVYP
Sbjct: 421 TLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWKYDMRKRYNLVDGISRHTVQVYP 480

Query: 481 KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKAS 540
           KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTE DIP NALKCGKAS
Sbjct: 481 KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTETDIPPNALKCGKAS 539

Query: 541 KY 543
           KY
Sbjct: 541 KY 539

BLAST of HG10000675 vs. NCBI nr
Match: XP_004148037.1 (L-ascorbate oxidase homolog [Cucumis sativus])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.4e-300
Identity = 499/543 (91.90%), Postives = 516/543 (95.03%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREGNLV  ++L + ++LVCCS G  WVNAEDPYRYFTFQVTYGTRAPLG  QKVILVNG
Sbjct: 1   MREGNLVMNLSLGIIVLLVCCSLG--WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIEC TNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYPIPAAE+DLLI DWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKLDSGI LPLP+ LLINGQPS TL+LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKP-ILTTIGILRY 300
           LVEVEGAHTVQE YESLDIHPGQSAAVLVSLNNLPKDYYFV SSRFTKP ILTT+GILRY
Sbjct: 241 LVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRY 300

Query: 301 EGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360
           EGSSI PSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE
Sbjct: 301 EGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360

Query: 361 NSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVN 420
           NSLRKIKGA+RCAVN+VSYVDPPTPLKLADWF I GVFDFKTFTD+PT RPAMLGVSVVN
Sbjct: 361 NSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVN 420

Query: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVY 480
           ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVG+GSGVW+Y MRKRYNLVDGISRHTVQVY
Sbjct: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVY 480

Query: 481 PKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           P+SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTE DIP NALKCGKA
Sbjct: 481 PRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 540

Query: 541 SKY 543
           SKY
Sbjct: 541 SKY 541

BLAST of HG10000675 vs. NCBI nr
Match: XP_008457817.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.7e-299
Identity = 496/542 (91.51%), Postives = 516/542 (95.20%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREG ++NL +L + II VCCS G  WVN EDPYRY+TFQVTYGTRAPLGV QKVILVNG
Sbjct: 1   MREGKMINL-SLGIMIIWVCCSLG--WVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIECATNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYP+P AE+DLLIGDWY+TDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRK+LDSG+ LPLP  LLINGQPS+TL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKQLDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE 300
           LVEVEGAHTVQ+ YESLDIHPGQSAAVLVSLN LPKDYYFV SSRFTKPILTT+GILRYE
Sbjct: 241 LVEVEGAHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKPILTTVGILRYE 300

Query: 301 GSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN 360
           GSSI PSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+N
Sbjct: 301 GSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKN 360

Query: 361 SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420
           SL KIKGAVRCAVNNVSYVDPPTPLKLADWF IGGVFDFKTFTDIPTPRPAMLGVSVVNI
Sbjct: 361 SLTKIKGAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420

Query: 421 TLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYP 480
           TLHDFVEIVFQN+ENTVQAWHFDGTSFYVVG+GSG W+Y MRKRYNLVDGISRHTVQVYP
Sbjct: 421 TLHDFVEIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYP 480

Query: 481 KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKAS 540
           +SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTEADIPLNALKCGKAS
Sbjct: 481 RSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKAS 539

Query: 541 KY 543
           KY
Sbjct: 541 KY 539

BLAST of HG10000675 vs. NCBI nr
Match: KAE8651923.1 (hypothetical protein Csa_006507 [Cucumis sativus])

HSP 1 Score: 1037.7 bits (2682), Expect = 3.5e-299
Identity = 498/542 (91.88%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREGNLV  ++L + ++LVCCS G  WVNAEDPYRYFTFQVTYGTRAPLG  QKVILVNG
Sbjct: 1   MREGNLVMNLSLGIIVLLVCCSLG--WVNAEDPYRYFTFQVTYGTRAPLGFNQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIEC TNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYPIPAAE+DLLI DWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPIPAAEVDLLISDWYNTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKLDSGI LPLP+ LLINGQPS TL+LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLDSGILLPLPNRLLINGQPSLTLTLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKP-ILTTIGILRY 300
           LVEVEGAHTVQE YESLDIHPGQSAAVLVSLNNLPKDYYFV SSRFTKP ILTT+GILRY
Sbjct: 241 LVEVEGAHTVQEVYESLDIHPGQSAAVLVSLNNLPKDYYFVVSSRFTKPTILTTVGILRY 300

Query: 301 EGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360
           EGSSI PSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE
Sbjct: 301 EGSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360

Query: 361 NSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVN 420
           NSLRKIKGA+RCAVN+VSYVDPPTPLKLADWF I GVFDFKTFTD+PT RPAMLGVSVVN
Sbjct: 361 NSLRKIKGALRCAVNSVSYVDPPTPLKLADWFKIDGVFDFKTFTDVPTSRPAMLGVSVVN 420

Query: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVY 480
           ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVG+GSGVW+Y MRKRYNLVDGISRHTVQVY
Sbjct: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGYGSGVWSYKMRKRYNLVDGISRHTVQVY 480

Query: 481 PKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           P+SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTE DIP NALKCGKA
Sbjct: 481 PRSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTETDIPPNALKCGKA 540

Query: 541 SK 542
           SK
Sbjct: 541 SK 540

BLAST of HG10000675 vs. NCBI nr
Match: TYJ99476.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1031.9 bits (2667), Expect = 1.9e-297
Identity = 493/536 (91.98%), Postives = 512/536 (95.52%), Query Frame = 0

Query: 9   LINLILGIIL--VCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPK 68
           +INL LGI++  VCCS G  WVN EDPYRY+TFQVTYGTRAPLGV QKVILVNGQFPAPK
Sbjct: 1   MINLSLGIMIIWVCCSLG--WVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQFPAPK 60

Query: 69  IECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD 128
           IECATNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD
Sbjct: 61  IECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD 120

Query: 129 QIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKK 188
           QIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYP+P AE+DLLIGDWY+TDHKTLRK+
Sbjct: 121 QIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKTLRKQ 180

Query: 189 LDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEG 248
           LDSG+ LPLP  LLINGQPS+TL LKKG TYKLRISNVGIATSINFRIQGHLMTLVEVEG
Sbjct: 181 LDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEG 240

Query: 249 AHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRP 308
           AHTVQ+ YESLDIHPGQSAAVLVSLN LPKDYYFV SSRFTKPILTT+GILRYEGSSI P
Sbjct: 241 AHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKPILTTVGILRYEGSSIPP 300

Query: 309 SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIK 368
           SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NSL KIK
Sbjct: 301 SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNSLTKIK 360

Query: 369 GAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV 428
           GAVRCAVNNVSYVDPPTPLKLADWF IGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV
Sbjct: 361 GAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV 420

Query: 429 EIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAI 488
           EIVFQN+ENTVQAWHFDGTSFYVVG+GSG W+Y MRKRYNLVDGISRHTVQVYP+SWTAI
Sbjct: 421 EIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPRSWTAI 480

Query: 489 LVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKASKY 543
           LVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTEADIPLNALKCGKASKY
Sbjct: 481 LVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASKY 534

BLAST of HG10000675 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 3.0e-160
Identity = 272/521 (52.21%), Postives = 354/521 (67.95%), Query Frame = 0

Query: 28  VNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNIIVNVINKLDEPF 87
           V+AEDPY +  + VTYGT +PLGV Q+VIL+NGQFP P I   +N+N+I+NV N LDEPF
Sbjct: 21  VHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEPF 80

Query: 88  LFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRAAGAFG 147
           L TW+GI+ R+  WQDG  GT CPI P +N+TY FQ KDQIG++ Y+P+    RAAG +G
Sbjct: 81  LLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGYG 140

Query: 148 AFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPLPDGLLINGQ--- 207
              +  R +I  PY  P  +  +LIGDWY   H  L+K LD G ++  PDG++ING+   
Sbjct: 141 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSGK 200

Query: 208 ----PSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEAYESLDIH 267
                +   +LK G+TY++RI NVG+ TSINFRIQ H M LVE+EG+H +Q  Y+SLD+H
Sbjct: 201 GDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSLDVH 260

Query: 268 PGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKPLPIGPTYQLHW 327
            GQ    +V+ N  PKDYY VASSRF K ++TT G+LRYEG     S  LP GP     W
Sbjct: 261 VGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQLPAGPV-GWAW 320

Query: 328 SMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAVRCAVNNVSYVD 387
           S+ Q R+ R NLTA+AARPNPQGS+HYG I + R + L N+  K+ G +R A+N VS+ +
Sbjct: 321 SLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRFALNGVSHTE 380

Query: 388 PPTPLKLADWFNIGG-VFDFKTFTDIPTP---RPAMLGVSVVNITLHDFVEIVFQNNENT 447
           P TPLKLA++F I   VF + T TD PTP   +   +  +V+NIT   FVE+VF+N+E +
Sbjct: 381 PETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTFVEVVFENHEKS 440

Query: 448 VQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAILVSLDNKGMW 507
           VQ+WH DG SF+ V    G WT + RK YNL+D +SRHTVQVYPK W AIL++ DN GMW
Sbjct: 441 VQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMW 500

Query: 508 NLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCG 538
           N+RS    R YLGQQLY  V + EKSL  E ++P  +L+CG
Sbjct: 501 NVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of HG10000675 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.4e-156
Identity = 262/534 (49.06%), Postives = 359/534 (67.23%), Query Frame = 0

Query: 17  ILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNII 76
           +L+C S G   V AEDPY YF + VTYGT APLGV Q+ IL+NGQFP P+I C +N+NI+
Sbjct: 11  LLLCLSVG---VIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIV 70

Query: 77  VNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPS 136
           VNV N LDEPFLFTW+G++ R+ SWQDG  GT CPI P  N+TY+FQVKDQIG+++YFP+
Sbjct: 71  VNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPT 130

Query: 137 INFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPLP 196
               RAAG +GA  +  R++I  P+  PA E ++ +GDWYN  HKTL+K LD G ++  P
Sbjct: 131 TALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRP 190

Query: 197 DGLLINGQPSTT-------LSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHT 256
           DG++ING+ +          +++ G+TY+ R  N+G+ +S+N R QGH M LVE+EG+HT
Sbjct: 191 DGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHT 250

Query: 257 VQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKP 316
           VQ  Y+SLD+H GQ  +VLV+ +  PKDYY V SSRF K  L+++ I+RY       S  
Sbjct: 251 VQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPE 310

Query: 317 LPIGP---TYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIK 376
           LP  P   T  + WSM Q R+ R NLTA+AARPNPQGS+HYG I + R + + NS+ ++ 
Sbjct: 311 LPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVG 370

Query: 377 GAVRCAVNNVSYVDPPTPLKLADWFN-IGGVFDFKTFTDIPTPRPAMLGV--SVVNITLH 436
           G +R  +N +S+ +  TPLKL ++F      F +    D     P+ L +  +V N T  
Sbjct: 371 GKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYR 430

Query: 437 DFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSW 496
           +FVEI+F+N+E T++ +H DG SF+ V    G W+ + RK YNLVDG+SR+ +QVYP SW
Sbjct: 431 NFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSW 490

Query: 497 TAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCG 538
            AI+++ DN GMWNLRS +W + YLG+QLYF V +  +SL  E +IP N   CG
Sbjct: 491 AAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCG 541

BLAST of HG10000675 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 268/557 (48.11%), Postives = 363/557 (65.17%), Query Frame = 0

Query: 7   VNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPK 66
           ++L  ++L +  V  SF  C+  A DPY ++ F+V+Y T +PLGV Q+VI +NG+FP P 
Sbjct: 1   MDLFKILLLVFFVNISF--CF--AADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPT 60

Query: 67  IECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD 126
           I   TN+N++VNV NKLDE  L  W+GI+QRR SWQDGVLGTNCPIPP  NWTY+FQVKD
Sbjct: 61  INVTTNENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKD 120

Query: 127 QIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKK 186
           QIG+F YFPS++FQRA+G FG+FV+ PR++I  P+  P  ++ + IGDWY  +H  LRK 
Sbjct: 121 QIGSFFYFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKA 180

Query: 187 LDSGISLPLPDGLLINGQ--------------PSTTLSLKKGQTYKLRISNVGIATSINF 246
           LD G  L +PDG+LING+                 T+++  G+TY+LR+SNVGI+TS+NF
Sbjct: 181 LDDGKDLGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNF 240

Query: 247 RIQGHLMTLVEVEGAHTVQEAYESLDIHPGQSAAVLVSLN-NLPKDYYFVASSRFTKPIL 306
           RIQGH + L E EG++TVQ+ Y SLDIH GQS + LV+++ N   DYY VAS+R     +
Sbjct: 241 RIQGHNLVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETI 300

Query: 307 ----TTIGILRYEGSSIRPSKPLPIGP--TYQLHWSMKQARTIRLNLTANAARPNPQGSF 366
               T +GIL+Y  S  +    LP GP   +   +SM QAR+IR N++A+ ARPNPQGSF
Sbjct: 301 WRRVTGVGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSF 360

Query: 367 HYGNITVVRRLILENSLR-KIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTD 426
            YG+I V    +L N     I G  R  +N +S+ +P TP++LAD   +  V+    F  
Sbjct: 361 KYGSINVTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKL-DFPK 420

Query: 427 IPTPRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKR 486
            P   PA +  S++N T   F+E+V QNN+  +Q++H  G +F+VVG   G WT + R  
Sbjct: 421 RPLTGPAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGT 480

Query: 487 YNLVDGISRHTVQVYPKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLY 542
           YN  DGI+R T+QVYP +W+AIL+SLDN G WNLR+      YLGQ+ Y RV N +++  
Sbjct: 481 YNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNK 540

BLAST of HG10000675 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.6e-145
Identity = 263/546 (48.17%), Postives = 346/546 (63.37%), Query Frame = 0

Query: 13  ILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATN 72
           +L   L+C +       A DP+  + F+V+Y T +PLGV Q+VI VNGQFP P +   TN
Sbjct: 7   LLASFLLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTN 66

Query: 73  DNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFT 132
            N++VNV N LDEP L TW GI+ RR SWQDGVLGTNCPIPP  N+TY+FQVKDQIG+F 
Sbjct: 67  YNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFF 126

Query: 133 YFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGIS 192
           Y PS+NFQRA+G FG  VI  R +I  P+P P  EL  +IGDWY  DHK LR+ LDSG  
Sbjct: 127 YSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKE 186

Query: 193 LPLPDGLLINGQ-------------PSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLM 252
           L +PDG+LING+                T  ++ G+TY++R+ NVGI+TS+NFRIQ H +
Sbjct: 187 LGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSL 246

Query: 253 TLVEVEGAHTVQEAYESLDIHPGQSAAVLVSLN-NLPKDYYFVASSRFTKPI----LTTI 312
            LVE EG +T Q  +   D+H GQS + LV+++ +   DYY VAS+RF        +T +
Sbjct: 247 LLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGV 306

Query: 313 GILRYEGSSIRPSKPLPIGPT-YQLHWS-MKQARTIRLNLTANAARPNPQGSFHYGNITV 372
            IL Y  S    S PLP+  T     WS M Q +TIR N +A+ ARPNPQGSFHYG I +
Sbjct: 307 AILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINI 366

Query: 373 VRRLILEN-SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPA 432
               IL +     I GA+R  +N +S+V+P TP++LAD   + G +    F D P  RP 
Sbjct: 367 TNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKL-DFPDRPFNRPL 426

Query: 433 MLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGI 492
            L  S++N T   F+++VFQNN+  +Q++H DG SF+VVG   G+W+ D +  YN  D I
Sbjct: 427 RLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAI 486

Query: 493 SRHTVQVYPKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPL 538
           SR T++VYP  WTA+L+SLDN G+WN+R     R YLG++ Y R+ N E+   TE D P 
Sbjct: 487 SRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPD 546

BLAST of HG10000675 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 4.1e-141
Identity = 250/530 (47.17%), Postives = 340/530 (64.15%), Query Frame = 0

Query: 30  AEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNIIVNVINKLDEPFLF 89
           A DPY  + F ++Y T +PLGV Q+VI VNG+FP P I   TN N+ VNV+N LDEP L 
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 90  TWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRAAGAFGAF 149
           TW G++ RR SWQDGVLGTNCPIPPN N+TY FQ+KDQIG++ Y PS+NFQRA+G FGA 
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 150 VIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPLPDGLLINGQ----- 209
           +I  R ++  P+  P  E+  +IGDWY  +H  LR+ LDSG  L +PDG+LING+     
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 210 --------PSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEAYES 269
                      T+++  G+TY++R+ NVGI+TS+NFRIQ H + L+E EG +T Q  +  
Sbjct: 203 NSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTD 262

Query: 270 LDIHPGQSAAVLVSLN-NLPKDYYFVASSRFTKPI----LTTIGILRYEGSSIRPSKPLP 329
            D+H GQS + LV+++ N   DYY VAS+RF        +T +GIL Y  S    S PLP
Sbjct: 263 FDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLP 322

Query: 330 IGPTYQLH-WS-MKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN-SLRKIKGA 389
           +  T   H WS M Q R I+ N +A+ ARPNPQGSFHYG I + R  IL +    KI G 
Sbjct: 323 VSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGK 382

Query: 390 VRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIP-TPRPAMLGVSVVNITLHDFVE 449
           +R  +N +S+V+P TP++LAD   + G +    F D P   +   L  S++N T   F++
Sbjct: 383 LRATLNGISFVNPSTPMRLADDHKVKGDY-MLDFPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 450 IVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAIL 509
           ++FQNN+  +Q++H DG +FYVV    G+W+ D    YN  D ++R TV+VYP +WTA+L
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 510 VSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCG 538
           +SLDN G+WN+R     R YLGQ+ Y R+ N E++  TE D P N + CG
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of HG10000675 vs. ExPASy TrEMBL
Match: A0A1S3C5Y6 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103497410 PE=3 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.3e-299
Identity = 496/542 (91.51%), Postives = 516/542 (95.20%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREG ++NL +L + II VCCS G  WVN EDPYRY+TFQVTYGTRAPLGV QKVILVNG
Sbjct: 1   MREGKMINL-SLGIMIIWVCCSLG--WVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIECATNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYP+P AE+DLLIGDWY+TDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRK+LDSG+ LPLP  LLINGQPS+TL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKQLDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE 300
           LVEVEGAHTVQ+ YESLDIHPGQSAAVLVSLN LPKDYYFV SSRFTKPILTT+GILRYE
Sbjct: 241 LVEVEGAHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKPILTTVGILRYE 300

Query: 301 GSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN 360
           GSSI PSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+N
Sbjct: 301 GSSIPPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKN 360

Query: 361 SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420
           SL KIKGAVRCAVNNVSYVDPPTPLKLADWF IGGVFDFKTFTDIPTPRPAMLGVSVVNI
Sbjct: 361 SLTKIKGAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420

Query: 421 TLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYP 480
           TLHDFVEIVFQN+ENTVQAWHFDGTSFYVVG+GSG W+Y MRKRYNLVDGISRHTVQVYP
Sbjct: 421 TLHDFVEIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYP 480

Query: 481 KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKAS 540
           +SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTEADIPLNALKCGKAS
Sbjct: 481 RSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKAS 539

Query: 541 KY 543
           KY
Sbjct: 541 KY 539

BLAST of HG10000675 vs. ExPASy TrEMBL
Match: A0A5D3BK26 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00410 PE=3 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 9.3e-298
Identity = 493/536 (91.98%), Postives = 512/536 (95.52%), Query Frame = 0

Query: 9   LINLILGIIL--VCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPK 68
           +INL LGI++  VCCS G  WVN EDPYRY+TFQVTYGTRAPLGV QKVILVNGQFPAPK
Sbjct: 1   MINLSLGIMIIWVCCSLG--WVNGEDPYRYYTFQVTYGTRAPLGVAQKVILVNGQFPAPK 60

Query: 69  IECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD 128
           IECATNDNIIVNVINKLDEPFLFTWSG+KQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD
Sbjct: 61  IECATNDNIIVNVINKLDEPFLFTWSGVKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKD 120

Query: 129 QIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKK 188
           QIGTFTYFPSINFQRA+G FGAFVIQPRSVITTPYP+P AE+DLLIGDWY+TDHKTLRK+
Sbjct: 121 QIGTFTYFPSINFQRASGGFGAFVIQPRSVITTPYPLPHAEIDLLIGDWYDTDHKTLRKQ 180

Query: 189 LDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEG 248
           LDSG+ LPLP  LLINGQPS+TL LKKG TYKLRISNVGIATSINFRIQGHLMTLVEVEG
Sbjct: 181 LDSGVLLPLPKRLLINGQPSSTLILKKGLTYKLRISNVGIATSINFRIQGHLMTLVEVEG 240

Query: 249 AHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRP 308
           AHTVQ+ YESLDIHPGQSAAVLVSLN LPKDYYFV SSRFTKPILTT+GILRYEGSSI P
Sbjct: 241 AHTVQDVYESLDIHPGQSAAVLVSLNRLPKDYYFVVSSRFTKPILTTVGILRYEGSSIPP 300

Query: 309 SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIK 368
           SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL+NSL KIK
Sbjct: 301 SKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILKNSLTKIK 360

Query: 369 GAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV 428
           GAVRCAVNNVSYVDPPTPLKLADWF IGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV
Sbjct: 361 GAVRCAVNNVSYVDPPTPLKLADWFKIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFV 420

Query: 429 EIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAI 488
           EIVFQN+ENTVQAWHFDGTSFYVVG+GSG W+Y MRKRYNLVDGISRHTVQVYP+SWTAI
Sbjct: 421 EIVFQNDENTVQAWHFDGTSFYVVGYGSGDWSYKMRKRYNLVDGISRHTVQVYPRSWTAI 480

Query: 489 LVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKASKY 543
           LVSLDNKGMWNLRSTIWWRNYLGQQLY RVWNDEKSLYTEADIPLNALKCGKASKY
Sbjct: 481 LVSLDNKGMWNLRSTIWWRNYLGQQLYVRVWNDEKSLYTEADIPLNALKCGKASKY 534

BLAST of HG10000675 vs. ExPASy TrEMBL
Match: A0A6J1D5Y7 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111017933 PE=3 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 3.5e-297
Identity = 491/542 (90.59%), Postives = 509/542 (93.91%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREGNLV   NLI+GI+L+CCS    WVNAEDPYRYFTFQV YGTRAPLGVKQKVILVNG
Sbjct: 1   MREGNLV---NLIVGIMLICCS--AYWVNAEDPYRYFTFQVMYGTRAPLGVKQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAPKIEC TNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPKIECVTNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRAAG FGAFVIQPRSVI TPYPIP AE DLLI DWY TDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRSVIATPYPIPDAEFDLLISDWYKTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           K+LRKKLDSGI LPLPD LLING P+TTL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KSLRKKLDSGIQLPLPDALLINGLPTTTLILKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYE 300
           LVEVEGAHT+QEAYESLDIHPGQSAAVL+SLNNLPKDY+FVASSRFTKPILTTIGI+RYE
Sbjct: 241 LVEVEGAHTMQEAYESLDIHPGQSAAVLISLNNLPKDYFFVASSRFTKPILTTIGIIRYE 300

Query: 301 GSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILEN 360
           GSSI PSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL N
Sbjct: 301 GSSILPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILAN 360

Query: 361 SLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNI 420
           SL KIKG +RCAVNNVSYVDP TPLKLADWFNI GVFD +TF D PTPRPA LGVSVVNI
Sbjct: 361 SLTKIKGTLRCAVNNVSYVDPTTPLKLADWFNIPGVFDIRTFIDKPTPRPATLGVSVVNI 420

Query: 421 TLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYP 480
           TLHD+VEI+FQNNENTVQ WHFDGTSFYVVG+G G+WTYDMR+RYNLVDGISRHTVQVYP
Sbjct: 421 TLHDYVEIIFQNNENTVQVWHFDGTSFYVVGYGPGIWTYDMRRRYNLVDGISRHTVQVYP 480

Query: 481 KSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKAS 540
           +SWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYF+VWNDEKSLYTEADIPLNALKCGKAS
Sbjct: 481 RSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFKVWNDEKSLYTEADIPLNALKCGKAS 537

Query: 541 KY 543
           KY
Sbjct: 541 KY 537

BLAST of HG10000675 vs. ExPASy TrEMBL
Match: A0A6J1G5M8 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111451104 PE=3 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 2.5e-295
Identity = 488/543 (89.87%), Postives = 510/543 (93.92%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREGNLV   NLILGIIL CC+F   WVNAEDPYRYFTFQVTYGTRAPLGV+QK+ILVNG
Sbjct: 1   MREGNLV---NLILGIILACCTFSTYWVNAEDPYRYFTFQVTYGTRAPLGVQQKIILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAP+IEC TNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPRIECETNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRAAG FGAFVIQPR+VITTPYPIP  ELDLLIGDWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVIQPRTVITTPYPIPNGELDLLIGDWYNTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRKKL+SG+ LPLP+GLLING+ STTL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKKLESGVQLPLPNGLLINGKSSTTLVLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNL-PKDYYFVASSRFTKPILTTIGILRY 300
           LVEVEGAHT+QE YESLDIHPGQSAAVLVSL NL  K YYFVAS+RFTKPIL  IGIL+Y
Sbjct: 241 LVEVEGAHTMQETYESLDIHPGQSAAVLVSLKNLASKSYYFVASTRFTKPILNAIGILKY 300

Query: 301 EGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360
           EGS+  PSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLI+E
Sbjct: 301 EGSTTPPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIIE 360

Query: 361 NSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVN 420
           NSL KIKGA+RCAVNNVSYVDPPTPLKLADWF I GVFD  TFTD PT RPAMLGVSVVN
Sbjct: 361 NSLTKIKGALRCAVNNVSYVDPPTPLKLADWFKIPGVFDINTFTDTPTSRPAMLGVSVVN 420

Query: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVY 480
           ITLHDFVEIVFQN E+TVQ+WHFDGTSFYVVG+GSGVWTY+MRKRYNLVDGISRHTVQVY
Sbjct: 421 ITLHDFVEIVFQNKESTVQSWHFDGTSFYVVGYGSGVWTYNMRKRYNLVDGISRHTVQVY 480

Query: 481 PKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           PKSWTAILVSLDNKGMWNLRST+WWRNYLGQQLYF+VWNDEKSLYTEADIPLNA+KCGKA
Sbjct: 481 PKSWTAILVSLDNKGMWNLRSTVWWRNYLGQQLYFKVWNDEKSLYTEADIPLNAIKCGKA 540

Query: 541 SKY 543
           SKY
Sbjct: 541 SKY 540

BLAST of HG10000675 vs. ExPASy TrEMBL
Match: A0A6J1KZC3 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111499628 PE=3 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 9.6e-295
Identity = 488/543 (89.87%), Postives = 509/543 (93.74%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           MREGNLV   NLILGIIL CC+    WVNAEDPYRYFTFQVTYGTRAPLGV+QKVILVNG
Sbjct: 1   MREGNLV---NLILGIILACCTLNTYWVNAEDPYRYFTFQVTYGTRAPLGVQQKVILVNG 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFPAP+IEC TNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY
Sbjct: 61  QFPAPRIECETNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDH 180
           KFQVKDQIGTFTYFPSINFQRAAG FGAFV+QPRSVITTPYPIP  ELDLLIGDWYNTDH
Sbjct: 121 KFQVKDQIGTFTYFPSINFQRAAGGFGAFVVQPRSVITTPYPIPNGELDLLIGDWYNTDH 180

Query: 181 KTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMT 240
           KTLRK+L+SGI LPLP+GLLING+ STTL LKKG TYKLRISNVGIATSINFRIQGHLMT
Sbjct: 181 KTLRKRLESGIQLPLPNGLLINGKSSTTLVLKKGLTYKLRISNVGIATSINFRIQGHLMT 240

Query: 241 LVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNL-PKDYYFVASSRFTKPILTTIGILRY 300
           LVEVEGAHT+QE YESLDIHPGQSAAVLVSL NL  K YYFVAS+RFTKPIL  IGIL+Y
Sbjct: 241 LVEVEGAHTMQETYESLDIHPGQSAAVLVSLKNLASKSYYFVASTRFTKPILNAIGILKY 300

Query: 301 EGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILE 360
           EGS+  PSKPLPIGPTY LHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLI+E
Sbjct: 301 EGSTTPPSKPLPIGPTYHLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIIE 360

Query: 361 NSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVN 420
           NSL KIKGA+RCAVNNVSYVDPPTPLKLADWF I GVFD  TFTD PT RPAMLGVSVVN
Sbjct: 361 NSLTKIKGALRCAVNNVSYVDPPTPLKLADWFKIPGVFDINTFTDTPTSRPAMLGVSVVN 420

Query: 421 ITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVY 480
           ITLHDFVEIVFQN E+TVQ+WHFDGTSFYVVG+GSGVWTY+MRKRYNLVDGISRHTVQVY
Sbjct: 421 ITLHDFVEIVFQNKESTVQSWHFDGTSFYVVGYGSGVWTYNMRKRYNLVDGISRHTVQVY 480

Query: 481 PKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           PKSWTAILVSLDNKGMWNLRST+WWRNYLGQQLYF+VWNDEKSLYTEADIPLNA+KCGKA
Sbjct: 481 PKSWTAILVSLDNKGMWNLRSTVWWRNYLGQQLYFKVWNDEKSLYTEADIPLNAIKCGKA 540

Query: 541 SKY 543
           SKY
Sbjct: 541 SKY 540

BLAST of HG10000675 vs. TAIR 10
Match: AT2G23630.1 (SKU5 similar 16 )

HSP 1 Score: 758.4 bits (1957), Expect = 3.8e-219
Identity = 356/529 (67.30%), Postives = 417/529 (78.83%), Query Frame = 0

Query: 12  LILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECAT 71
           L+LG +L  C      +NAEDPY +FT+ VTYGTR+PLGV Q+VIL+NGQFP P IE  T
Sbjct: 7   LLLGFLLAYCFSSVFVINAEDPYLFFTWTVTYGTRSPLGVPQQVILINGQFPGPPIEGVT 66

Query: 72  NDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTF 131
           N+NI+VNVINKLDEPFL TW+GIKQR+ SWQDGVLGTNCPI P S+WTY FQ+KDQIGT+
Sbjct: 67  NNNIVVNVINKLDEPFLITWNGIKQRKMSWQDGVLGTNCPIQPKSSWTYHFQLKDQIGTY 126

Query: 132 TYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGI 191
            YF S +  RA+GAFGA  +  RSVI  PYP P A+  LL+ DWY   HK L+++LDS  
Sbjct: 127 AYFASTSMHRASGAFGALNVNQRSVIFVPYPKPDADFTLLVSDWYKMGHKELQRRLDSSR 186

Query: 192 SLPLPDGLLING-QPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTV 251
           +LP PDGLLING       + + G+ Y+ RISNVGI+TSINFRIQGH+MTLVEVEG+HT+
Sbjct: 187 ALPPPDGLLINGASKGLVFTGQHGKIYRFRISNVGISTSINFRIQGHMMTLVEVEGSHTL 246

Query: 252 QEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKPL 311
           QE YESLDIH GQS  VLV+L    KDY+ VAS+RFTKPILTT GIL Y+GS IRPS PL
Sbjct: 247 QEVYESLDIHVGQSVTVLVTLKAPVKDYFIVASTRFTKPILTTTGILSYQGSKIRPSHPL 306

Query: 312 PIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAVR 371
           PIGPTY +HWSMKQARTIRLNLTANAARPNPQGSFHYG I + R  +L N    I G +R
Sbjct: 307 PIGPTYHIHWSMKQARTIRLNLTANAARPNPQGSFHYGTIPINRTFVLANGRAMINGKLR 366

Query: 372 CAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIVF 431
             VN VSYV+P TPLKLADWFNI GVF+FKT  +IPTP P++LG SV ++ LH++VE VF
Sbjct: 367 YTVNRVSYVNPATPLKLADWFNIPGVFNFKTIMNIPTPGPSILGTSVFDVALHEYVEFVF 426

Query: 432 QNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAILVSL 491
           QNNE ++Q+WH DGTS YVVG+GSG W    R+ YNLVD +SRHT QVYP SWT+ILVSL
Sbjct: 427 QNNEGSIQSWHLDGTSAYVVGYGSGTWNMAKRRGYNLVDAVSRHTFQVYPMSWTSILVSL 486

Query: 492 DNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           DNKGMWNLRS IW R YLGQ+LY RVWN+EKSLYTE++ P+N L CGKA
Sbjct: 487 DNKGMWNLRSQIWSRRYLGQELYVRVWNNEKSLYTESEPPVNVLFCGKA 535

BLAST of HG10000675 vs. TAIR 10
Match: AT4G37160.1 (SKU5 similar 15 )

HSP 1 Score: 758.4 bits (1957), Expect = 3.8e-219
Identity = 357/543 (65.75%), Postives = 424/543 (78.08%), Query Frame = 0

Query: 1   MREGNLVNLINLILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNG 60
           M++ NL+ +  L +G +      G   VNAEDPY ++T+ VTYGTR+PLGV Q+VIL+NG
Sbjct: 1   MKQTNLL-VCKLFIGALF---WLGSVLVNAEDPYMFYTWTVTYGTRSPLGVPQQVILING 60

Query: 61  QFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTY 120
           QFP P IE  TN+NI+VN+INKLDEPFL TW+G+KQRRTSWQDGVLGTNCPI PNSNWTY
Sbjct: 61  QFPGPAIEAVTNNNIVVNLINKLDEPFLITWNGVKQRRTSWQDGVLGTNCPIQPNSNWTY 120

Query: 121 KFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWY-NTD 180
           +FQ+KDQIGT+TYF S +  RA+GAFGA  I  RSVITTPYP P  +  LL+ DW+ N  
Sbjct: 121 QFQLKDQIGTYTYFASTSLHRASGAFGALNINQRSVITTPYPTPDGDFTLLVSDWFSNMT 180

Query: 181 HKTLRKKLDSGISLPLPDGLLING-QPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHL 240
           HK LRK LD+G +LPLPD LLING       + ++G+TYK R+SNVGIATSINFRIQ H 
Sbjct: 181 HKDLRKSLDAGSALPLPDALLINGVSKGLIFTGQQGKTYKFRVSNVGIATSINFRIQNHT 240

Query: 241 MTLVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILR 300
           M+L+EVEGAHT+QE+YESLD+H GQS  VLV+L    +DY+ VAS+RFTKP+LTT   LR
Sbjct: 241 MSLIEVEGAHTLQESYESLDVHVGQSMTVLVTLKASVRDYFIVASTRFTKPVLTTTASLR 300

Query: 301 YEGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL 360
           Y+GS      PLPIGPTY +HWSMKQARTIR+NLTANAARPNPQGSFHYG I + R L+L
Sbjct: 301 YQGSKNAAYGPLPIGPTYHIHWSMKQARTIRMNLTANAARPNPQGSFHYGTIPINRTLVL 360

Query: 361 ENSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVV 420
            N+   I G +R  VN +SY++P TPLKLADW+NI GVFDFKT    PT  PA +G SV+
Sbjct: 361 ANAATLIYGKLRYTVNRISYINPTTPLKLADWYNISGVFDFKTIISTPTTGPAHIGTSVI 420

Query: 421 NITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQV 480
           ++ LH+FVEIVFQN+E ++Q+WH DGTS Y VG+GSG W   MRKRYNLVD + RHT QV
Sbjct: 421 DVELHEFVEIVFQNDERSIQSWHMDGTSAYAVGYGSGTWNVTMRKRYNLVDAVPRHTFQV 480

Query: 481 YPKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGK 540
           YP SWT ILVSLDNKGMWNLRS IW R YLGQ+LY RVWNDEKSLYTEA+ PLN L CGK
Sbjct: 481 YPLSWTTILVSLDNKGMWNLRSQIWSRRYLGQELYVRVWNDEKSLYTEAEPPLNVLYCGK 539

Query: 541 ASK 542
           A +
Sbjct: 541 AKR 539

BLAST of HG10000675 vs. TAIR 10
Match: AT5G66920.1 (SKU5 similar 17 )

HSP 1 Score: 660.6 bits (1703), Expect = 1.1e-189
Identity = 317/541 (58.60%), Postives = 388/541 (71.72%), Query Frame = 0

Query: 1   MREGNLVNLIN--LILGIILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILV 60
           M      +L+N  L+LG + +  S     V  E PY+++T+ VTYG  +PLGV Q+VIL+
Sbjct: 3   MASRKTTSLLNHLLLLGALTLLSSL--VIVKGESPYKFYTWTVTYGIISPLGVPQQVILI 62

Query: 61  NGQFPAPKIECATNDNIIVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNW 120
           NGQFP PK+E  TNDNII+N+INKLD+PFL TW+GIKQR+ SWQDGVLGTNCPI PNSN+
Sbjct: 63  NGQFPGPKLEVVTNDNIILNLINKLDQPFLLTWNGIKQRKNSWQDGVLGTNCPIQPNSNF 122

Query: 121 TYKFQVKDQIGTFTYFPSINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNT 180
           TYKFQ KDQIGTF YFPS  F +AAG FGA  +  R  I  PYP+P A+  LL+GDW+ T
Sbjct: 123 TYKFQTKDQIGTFNYFPSTAFHKAAGGFGAINVYARPGIPIPYPLPTADFTLLVGDWFKT 182

Query: 181 DHKTLRKKLDSGISLPLPDGLLINGQPSTTLSLKKGQTYKLRISNVGIATSINFRIQGHL 240
           +HKTL+++LDSG  LP PDG+LINGQ  +T S  +G+TY LRISNVG++++ NFRIQGH 
Sbjct: 183 NHKTLQQRLDSGGVLPFPDGMLINGQTQSTFSGDQGKTYMLRISNVGLSSTFNFRIQGHT 242

Query: 241 MTLVEVEGAHTVQEAYESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILR 300
           M +VEVEG+H +Q  Y+SLDIH GQS AVLV+LN  PKDYY VAS+RF +  L+ +G+LR
Sbjct: 243 MKVVEVEGSHVIQTDYDSLDIHVGQSLAVLVTLNQSPKDYYIVASTRFIRSKLSVMGLLR 302

Query: 301 YEGSSIRPSKPLPIGPTYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLIL 360
           Y  S +  S   P  P  +L WSM+QART R NLTANAARPNPQGSFHYG I+  +  + 
Sbjct: 303 YSNSRVPASGDPPALPPGELVWSMRQARTFRWNLTANAARPNPQGSFHYGMISPTKTFVF 362

Query: 361 ENSLRKIKGAVRCAVNNVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVV 420
            NS   I G  R AVN VSYV   TPLKLAD F I GVF       +P+  P  +  SVV
Sbjct: 363 SNSAPLINGKQRYAVNGVSYVKSETPLKLADHFGISGVFSTNAIQSVPSNSPPTVATSVV 422

Query: 421 NITLHDFVEIVFQNNENTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQV 480
             + HDF+EIVFQNNE ++Q+WH DG  F+VVGFGSG WT   R  +NLVD ++RHT QV
Sbjct: 423 QTSHHDFLEIVFQNNEKSMQSWHLDGYDFWVVGFGSGQWTPAKRSLHNLVDALTRHTTQV 482

Query: 481 YPKSWTAILVSLDNKGMWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGK 540
           YP+SWT ILVSLDN+GMWN+RS IW R Y GQQ Y +VWN  +SL  E + P N   CGK
Sbjct: 483 YPESWTTILVSLDNQGMWNMRSAIWERQYSGQQFYLKVWNSVQSLANEYNPPDNLQLCGK 541

BLAST of HG10000675 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 598.6 bits (1542), Expect = 5.0e-171
Identity = 285/514 (55.45%), Postives = 359/514 (69.84%), Query Frame = 0

Query: 28  VNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNIIVNVINKLDEPF 87
           V AEDPYR+F + +TYG   PLGV+Q+ IL+NG FP P I   TNDN+I+NV N LDEPF
Sbjct: 21  VTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLIINVYNSLDEPF 80

Query: 88  LFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFPSINFQRAAGAFG 147
           L +W+GI+QRR S+ DGV GT CPIPP  N+TY  Q+KDQIG+F YFPS+ F +AAG FG
Sbjct: 81  LLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSLGFHKAAGGFG 140

Query: 148 AFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPLPDGLLINGQPS- 207
              I  R  I  P+P PA +  +LIGDWY  +H  LR +LD+G  LPLPDG+LING+ S 
Sbjct: 141 GIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPDGILINGRSSG 200

Query: 208 TTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEAYESLDIHPGQSAA 267
            TL++++G+TY+ RISNVG+  S+NFRIQ H M +VEVEG HT+Q  + SLD+H GQS +
Sbjct: 201 ATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFSSLDVHVGQSYS 260

Query: 268 VLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKPLPIGPTYQLHWSMKQAR 327
           VLV+ +  P+DYY V SSRFT  +LTT GI RY  S+   S P+P GPT Q+ WS+ QAR
Sbjct: 261 VLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPTIQIDWSLNQAR 320

Query: 328 TIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAVRCAVNNVSYVDPPTPLK 387
            IR NL+A+  RPNPQGS+HYG I   R + L +S  ++ G  R AVN+VS+    TPLK
Sbjct: 321 AIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNSVSFKPADTPLK 380

Query: 388 LADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIVFQNNENTVQAWHFDGTS 447
           +AD+F I GV+   +    PT     L  SV+ +    FVEI+F+N+E+ VQ+WH DG S
Sbjct: 381 IADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSEDIVQSWHLDGYS 440

Query: 448 FYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAILVSLDNKGMWNLRSTIWWRN 507
           F+VVG   G W+ D R  YNL D ++R TVQVYP SWTAIL++LDN GMWNLRS  W R 
Sbjct: 441 FWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGMWNLRSEFWARQ 500

Query: 508 YLGQQLYFRVWNDEKSLYTEADIPLNALKCGKAS 541
           YLGQQLY RV+    SL  E  IP NAL CG+AS
Sbjct: 501 YLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRAS 534

BLAST of HG10000675 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 597.8 bits (1540), Expect = 8.6e-171
Identity = 287/525 (54.67%), Postives = 364/525 (69.33%), Query Frame = 0

Query: 16  IILVCCSFGGCWVNAEDPYRYFTFQVTYGTRAPLGVKQKVILVNGQFPAPKIECATNDNI 75
           I+L C SF    V AE PYR+F + VTYG   PLGV+Q+ IL+NGQFP P I   TNDN+
Sbjct: 11  ILLFCLSFFAA-VTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNL 70

Query: 76  IVNVINKLDEPFLFTWSGIKQRRTSWQDGVLGTNCPIPPNSNWTYKFQVKDQIGTFTYFP 135
           I+NV N LDEPFL +W+G++ RR S+ DG+ GT CPIPP SN+TY  QVKDQIG+F YFP
Sbjct: 71  IINVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFP 130

Query: 136 SINFQRAAGAFGAFVIQPRSVITTPYPIPAAELDLLIGDWYNTDHKTLRKKLDSGISLPL 195
           S+ F +AAG FG   I  R  I  P+  PA +  +LIGDWY  +H  L+ +LD G  LP 
Sbjct: 131 SLAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPS 190

Query: 196 PDGLLINGQPS-TTLSLKKGQTYKLRISNVGIATSINFRIQGHLMTLVEVEGAHTVQEAY 255
           PDG+LING+ +  TL++++G+TY+LRISNVG+  S+NFRIQ H M LVEVEG HT+Q  +
Sbjct: 191 PDGILINGRSNGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTMF 250

Query: 256 ESLDIHPGQSAAVLVSLNNLPKDYYFVASSRFTKPILTTIGILRYEGSSIRPSKPLPIGP 315
            SLD+H GQS +VL++ +  P+DYY V SSRFT  I+TT G+LRY GSS   S P+P GP
Sbjct: 251 SSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGGP 310

Query: 316 TYQLHWSMKQARTIRLNLTANAARPNPQGSFHYGNITVVRRLILENSLRKIKGAVRCAVN 375
           T Q+ WS+ QAR IR NLTA+  RPNPQGS+HYG I ++R ++  +S  +I G  R  VN
Sbjct: 311 TIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGVN 370

Query: 376 NVSYVDPPTPLKLADWFNIGGVFDFKTFTDIPTPRPAMLGVSVVNITLHDFVEIVFQNNE 435
           +VS+V   TPLKLAD+F I GV+   + +D PT     L  SV+ +    F+EIVF+N E
Sbjct: 371 SVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQE 430

Query: 436 NTVQAWHFDGTSFYVVGFGSGVWTYDMRKRYNLVDGISRHTVQVYPKSWTAILVSLDNKG 495
           + VQ++H +G SF+VVG   G W    R  YNL D +SR TVQVYPKSWTAI ++LDN G
Sbjct: 431 DIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNVG 490

Query: 496 MWNLRSTIWWRNYLGQQLYFRVWNDEKSLYTEADIPLNALKCGKA 540
           MWNLRS  W R YLGQQLY RV+    SL  E  IP N+  CG+A
Sbjct: 491 MWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRA 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902225.15.4e-30894.65L-ascorbate oxidase homolog [Benincasa hispida][more]
XP_004148037.15.4e-30091.90L-ascorbate oxidase homolog [Cucumis sativus][more]
XP_008457817.12.7e-29991.51PREDICTED: L-ascorbate oxidase homolog [Cucumis melo][more]
KAE8651923.13.5e-29991.88hypothetical protein Csa_006507 [Cucumis sativus][more]
TYJ99476.11.9e-29791.98L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q006243.0e-16052.21L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291623.4e-15649.06L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.3e-14748.11Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.6e-14548.17Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN64.1e-14147.17Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A1S3C5Y61.3e-29991.51L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103497410 PE=3 SV=1[more]
A0A5D3BK269.3e-29891.98L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1D5Y73.5e-29790.59L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111017933 PE=3 ... [more]
A0A6J1G5M82.5e-29589.87L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111451104 PE=3 S... [more]
A0A6J1KZC39.6e-29589.87L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111499628 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G23630.13.8e-21967.30SKU5 similar 16 [more]
AT4G37160.13.8e-21965.75SKU5 similar 15 [more]
AT5G66920.11.1e-18958.60SKU5 similar 17 [more]
AT1G76160.15.0e-17155.45SKU5 similar 5 [more]
AT1G41830.18.6e-17154.67SKU5-similar 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 33..156
e-value: 3.4E-35
score: 122.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 348..541
e-value: 1.2E-37
score: 131.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 166..338
e-value: 4.2E-31
score: 110.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 166..339
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 353..536
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 29..181
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 41..154
e-value: 4.0E-41
score: 139.8
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 386..520
e-value: 2.7E-26
score: 91.9
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 170..301
e-value: 1.6E-33
score: 116.1
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 10..539
NoneNo IPR availablePANTHERPTHR11709:SF245SKU5 SIMILAR 16coord: 10..539
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..26
score: 5.0
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 36..151
e-value: 2.21396E-71
score: 221.897

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000675.1HG10000675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity