HG10000583 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000583 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1500.7 bits (3884), Expect = 0.0e+00
Identity = 757/772 (98.06%), Postives = 761/772 (98.58%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MAAFLS SLSPFFFLLLL L STVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES
Sbjct: 1   MAAFLSTSLSPFFFLLLLTLHSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120
           PKILHVYDTVFHGFSATLTQ QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL
Sbjct: 61  PKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180
           WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA
Sbjct: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180

Query: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240
           RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI
Sbjct: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240

Query: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDP 300
           AKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLDP
Sbjct: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360
           IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI
Sbjct: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360

Query: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420
           SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG
Sbjct: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420

Query: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQ 480
           LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NP ATIAFQ
Sbjct: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIATIAFQ 480

Query: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTE 540
           GTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTE
Sbjct: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTE 540

Query: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600
           FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS
Sbjct: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600

Query: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLP 660
           TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPV CPAKKPLP
Sbjct: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAKKPLP 660

Query: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFS 720
           ENLNYPSIV+VFSSLSKGWSTKSFIRT TNVGP+NS YRAKIEAPKGVTVKVKPSKLVFS
Sbjct: 661 ENLNYPSIVSVFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSKLVFS 720

Query: 721 ATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
            TVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 TTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

BLAST of HG10000583 vs. NCBI nr
Match: XP_004147036.1 (subtilisin-like protease SBT1.6 [Cucumis sativus] >KGN61925.1 hypothetical protein Csa_006141 [Cucumis sativus])

HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 738/772 (95.60%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MAAFLS SLS FFF  LL L+STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF +S
Sbjct: 1   MAAFLSTSLSSFFFSFLL-LLSTVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQS 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120
           P+ILHVYDTVFHGFSATLTQDQVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL
Sbjct: 61  PQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180
           WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA
Sbjct: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180

Query: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240
           RFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYASGI
Sbjct: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 240

Query: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDP 300
           AKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLDP
Sbjct: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360
           IAIG+YGAAS+GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGRKI
Sbjct: 301 IAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKI 360

Query: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420
            GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V GKIVICDRGSSPRVAKG
Sbjct: 361 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKG 420

Query: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQ 480
           LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQ
Sbjct: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 480

Query: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTE 540
           GTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFD RKTE
Sbjct: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRKTE 540

Query: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600
           FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK S
Sbjct: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKPS 600

Query: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLP 660
           TPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKPLP
Sbjct: 601 TPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLP 660

Query: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFS 720
           ENLNYPSIV VFSSLSKGWSTKSFIRTATNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS
Sbjct: 661 ENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFS 720

Query: 721 ATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
            TVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 TTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of HG10000583 vs. NCBI nr
Match: KAG6571024.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 741/773 (95.86%), Postives = 756/773 (97.80%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAE 60
           MAA+LS  L  FFF LL++   TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAE
Sbjct: 1   MAAYLS-PLFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAE 60

Query: 61  SPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120
           S KILHVYDTVFHGFSATLTQ QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG
Sbjct: 61  SHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120

Query: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVG 180
           LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIVG
Sbjct: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVG 180

Query: 181 ARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 240
           ARFFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASG
Sbjct: 181 ARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASG 240

Query: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLD 300
           IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLD
Sbjct: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 300

Query: 301 PIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRK 360
           PIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR+
Sbjct: 301 PIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRR 360

Query: 361 ISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAK 420
           ISGVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK VAGKIVICDRGSSPRVAK
Sbjct: 361 ISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAK 420

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAF 480
           GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSSPNPTATIAF
Sbjct: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAF 480

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
           QGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKT
Sbjct: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKT 540

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKA 600
           EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKA
Sbjct: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKA 600

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL 660
           STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL
Sbjct: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL 660

Query: 661 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 720
           PENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPANSVYRAKIEAPKGVTVKVKPSKLVF
Sbjct: 661 PENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 720

Query: 721 SATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           SA  KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 SAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 772

BLAST of HG10000583 vs. NCBI nr
Match: XP_008457681.1 (PREDICTED: subtilisin-like protease SBT1.6 [Cucumis melo])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/772 (95.60%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MAAFLS SLSPFFF  LL L+STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF +S
Sbjct: 1   MAAFLSTSLSPFFFSFLL-LLSTVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQS 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120
           P+ILHVYDTVFHGFSATLTQ+QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL
Sbjct: 61  PQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180
           WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA
Sbjct: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180

Query: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240
           RFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYASGI
Sbjct: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 240

Query: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDP 300
           AKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLDP
Sbjct: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360
           IAIGAYGAAS+GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGRKI
Sbjct: 301 IAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKI 360

Query: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420
            GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V GKIVICDRGSSPRVAKG
Sbjct: 361 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKG 420

Query: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQ 480
           LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQ
Sbjct: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 480

Query: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTE 540
           GTIIGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTE
Sbjct: 481 GTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTE 540

Query: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600
           FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEESTGK S
Sbjct: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGKPS 600

Query: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLP 660
           TPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKPLP
Sbjct: 601 TPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLP 660

Query: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFS 720
           ENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS
Sbjct: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFS 720

Query: 721 ATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
            TVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 TTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of HG10000583 vs. NCBI nr
Match: XP_022943985.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 740/774 (95.61%), Postives = 756/774 (97.67%), Query Frame = 0

Query: 1   MAAFLS-ISLSPFFFLLLLILISTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAA+LS +    FFF LL++   TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ES KILHVYDTVFHGFSATLTQ QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+AS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 KISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVA 420
           +ISGVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK VAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           FSA  KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of HG10000583 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 567/764 (74.21%), Postives = 664/764 (86.91%), Query Frame = 0

Query: 15  LLLLILIS----TVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTV 74
           ++LL+ +S    + +A    KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TV
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTV 65

Query: 75  FHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 134
           FHGFSA +T D+ D +  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDV
Sbjct: 66  FHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDV 125

Query: 135 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAG 194
           IIGVFDTGI PERRSFSD+NLGPIP+RW+GVCE+G +F+ +NCNRKI+GARFF+KG +  
Sbjct: 126 IIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ-- 185

Query: 195 SNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARL 254
              A  I GIN T+E+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPKAR+
Sbjct: 186 ---AAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARI 245

Query: 255 AVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 314
           A YKVCWK+SGC DSDILAAFD AV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS
Sbjct: 246 AAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 305

Query: 315 RGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSLYAGAP 374
           +G+FVSSSAGN+GPNGMSVTNLAPWVTTVGA TIDRNFPA   LG+G ++ GVSLYAG P
Sbjct: 306 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVP 365

Query: 375 LNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGLVVKKAGGVG 434
           LNG M+P+VYPGKSG+ S SLCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVG
Sbjct: 366 LNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVG 425

Query: 435 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQGTIIGIKPAP 494
           MILANG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS PNP A+I F+GTI+GIKPAP
Sbjct: 426 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 485

Query: 495 VVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 554
           V+ASFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMA
Sbjct: 486 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 545

Query: 555 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTPYDFGAGHV 614
           CPHVSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK++TPYD+G+GH+
Sbjct: 546 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHL 605

Query: 615 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCP-AKKPLPENLNYPSIV 674
           NLG AM+PGLVYDITN DYI FLCSIGYGPK IQVITRTPV CP  +KP P NLNYPSI 
Sbjct: 606 NLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSIT 665

Query: 675 AVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSATVKKQSFV 734
           AVF +  +G  +K+ IRTATNVG A +VYRA+IE+P+GVTV VKP +LVF++ VK++S+ 
Sbjct: 666 AVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYA 725

Query: 735 VAISADNQNLALGDVGAVFGWLSWSD-GKHVVRSPLVVTQLEPL 773
           V ++ + +N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 726 VTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of HG10000583 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 3.9e-246
Identity = 452/779 (58.02%), Postives = 548/779 (70.35%), Query Frame = 0

Query: 12  FFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESPKILHVY 71
           FFFLL L   S+ ++ S   T+I  VD  +KPS+FPTH+HWYTS  A      P I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 72  DTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 131
           DTVFHGFSA LT      +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 132 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSK 191
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 192 GHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 251
           G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 252 PKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIAIGA 311
           PKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 312 YGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSL 371
           +GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ ISGVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 372 YAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGL 431
           Y G  L+ G MYPLVY G        S SLC+E SLDP  V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 432 VVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SSPNPTA 491
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 492 TIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFD 551
           TI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 552 TRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEES 611
            R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 612 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPA 671
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DYINFLC+  Y    I  ITR    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 672 KKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVK 731
            +      NLNYPS   VF    +   +  FIRT TNVG ++SVY  KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 732 PSKLVFSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of HG10000583 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 9.0e-211
Identity = 393/768 (51.17%), Postives = 523/768 (68.10%), Query Frame = 0

Query: 7   ISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS---EFAESPKI 66
           +S + FF LL L      S+ S   T+I  + +   PS F  H +WY S     ++S ++
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65

Query: 67  LHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGLWS 126
           L+ Y+   HGFS  LTQ++ D++   P V++V  + R +LHTTR+P FLGL  +   L+ 
Sbjct: 66  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125

Query: 127 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARF 186
           ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA  CNRK++GARF
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185

Query: 187 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 246
           F++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG A+
Sbjct: 186 FARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 245

Query: 247 GVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIA 306
           G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D +A
Sbjct: 246 GMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDGVA 305

Query: 307 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISG 366
           IGA+ A  RG+ VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG+  +G
Sbjct: 306 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 365

Query: 367 VSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGL 426
           VSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV KG 
Sbjct: 366 VSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGD 425

Query: 427 VVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQG 486
           VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++ PNPTA+I+  G
Sbjct: 426 VVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILG 485

Query: 487 TIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEF 546
           T++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL  D+R+ EF
Sbjct: 486 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 545

Query: 547 NILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAST 606
           NI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +TGK ST
Sbjct: 546 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 605

Query: 607 PYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTC-PAKKPLP 666
           P+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y    I+ ++R   TC P+K    
Sbjct: 606 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 665

Query: 667 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEA-PKGVTVKVKPSKLVF 726
            +LNYPS    F+    G     + RT T+VG A + Y  K+ +   GV + V+P+ L F
Sbjct: 666 ADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNF 725

Query: 727 SATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 768
               +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 726 KEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of HG10000583 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 4.3e-205
Identity = 383/766 (50.00%), Postives = 508/766 (66.32%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MA+  S S S       L L+   +A    KT+I RV+   KP  F TH+ WYTS+    
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK---KTYIIRVNHSDKPESFLTHHDWYTSQLNSE 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDT-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120
             +L+ Y T FHGFSA L   + D+ +    S+L +FED    LHTTR+P+FLGL ++ G
Sbjct: 61  SSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 120

Query: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVG 180
           +       + VIIGV DTG+ PE RSF D ++  IP +WKG CE+G+ F +K CN+K++G
Sbjct: 121 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 180

Query: 181 ARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 240
           AR FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G
Sbjct: 181 ARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 240

Query: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLD 300
            A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D
Sbjct: 241 TARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRD 300

Query: 301 PIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRK 360
            IAIGA+ A  RGVFVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG++
Sbjct: 301 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 360

Query: 361 ISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAK 420
           ++GVSLY+G  +      LVY  K    S +LC+  SLD   V GKIV+CDRG + RV K
Sbjct: 361 LTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEK 420

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAF 480
           G VV+ AGG+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S   PTA + F
Sbjct: 421 GAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVF 480

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
           +GT++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T
Sbjct: 481 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 540

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKA 600
           +FNI+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    
Sbjct: 541 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 600

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYG-PKMIQVITRTPVTCPAKKP 660
           S PY  G+GHV+   A+ PGLVYDI+  +YI FLCS+ Y    ++ ++ R  V C  K  
Sbjct: 601 SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
            P  LNYPS    FS L  G     + R  TNVG A+SVY+  +     V + VKPSKL 
Sbjct: 661 DPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLS 720

Query: 721 FSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 765
           F +  +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 721 FKSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of HG10000583 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.0e-198
Identity = 384/789 (48.67%), Postives = 507/789 (64.26%), Query Frame = 0

Query: 11  PFFFLLLLILISTVSAD-----SPLKTFIFRVDRFSKPSVFPTHYHWYTS---------- 70
           PF F++L I +  + A+     S  KT++  +D+ + P  +  H  WY+S          
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 71  --EFAESPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLG 130
             E   + +IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 131 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAK 190
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F  +
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 191 NCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQA 250
           NCNRKIVGAR F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 251 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDG 310
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 311 VSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAV 370
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 371 VTLGNGRKISGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKAVAGKIV 430
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + VAGKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 431 ICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYA 490
           ICDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 491 SSSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAV 550
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 551 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 610
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 611 RQPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVIT 670
            +P+T+ S    S+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 671 R-TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIE 730
           + +  TC    AK   P NLNYP+I A+F   +      +  RT TNVGP  S Y+  + 
Sbjct: 670 KHSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVS 729

Query: 731 APKGVTVKVKPSKLVFSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSP 773
             KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP
Sbjct: 730 PFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSP 780

BLAST of HG10000583 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1469.1 bits (3802), Expect = 0.0e+00
Identity = 738/772 (95.60%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MAAFLS SLS FFF  LL L+STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF +S
Sbjct: 1   MAAFLSTSLSSFFFSFLL-LLSTVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQS 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120
           P+ILHVYDTVFHGFSATLTQDQVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL
Sbjct: 61  PQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180
           WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA
Sbjct: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180

Query: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240
           RFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYASGI
Sbjct: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 240

Query: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDP 300
           AKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLDP
Sbjct: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360
           IAIG+YGAAS+GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGRKI
Sbjct: 301 IAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKI 360

Query: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420
            GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V GKIVICDRGSSPRVAKG
Sbjct: 361 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKG 420

Query: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQ 480
           LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQ
Sbjct: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 480

Query: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTE 540
           GTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFD RKTE
Sbjct: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRKTE 540

Query: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600
           FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK S
Sbjct: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKPS 600

Query: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLP 660
           TPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKPLP
Sbjct: 601 TPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLP 660

Query: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFS 720
           ENLNYPSIV VFSSLSKGWSTKSFIRTATNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS
Sbjct: 661 ENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFS 720

Query: 721 ATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
            TVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 TTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of HG10000583 vs. ExPASy TrEMBL
Match: A0A1S3C7D0 (subtilisin-like protease SBT1.6 OS=Cucumis melo OX=3656 GN=LOC103497323 PE=3 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 738/772 (95.60%), Postives = 752/772 (97.41%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MAAFLS SLSPFFF  LL L+STVS+ SPLKTFI R+DRFSKPSVFPTHYHWYTSEF +S
Sbjct: 1   MAAFLSTSLSPFFFSFLL-LLSTVSSLSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFTQS 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120
           P+ILHVYDTVFHGFSATLTQ+QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL
Sbjct: 61  PQILHVYDTVFHGFSATLTQEQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGL 120

Query: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180
           WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA
Sbjct: 121 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGA 180

Query: 181 RFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 240
           RFFSKGHEAG+NAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRH+FQASLEGYASGI
Sbjct: 181 RFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYASGI 240

Query: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDP 300
           AKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLDP
Sbjct: 241 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDP 300

Query: 301 IAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKI 360
           IAIGAYGAAS+GVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFP+VVTLGNGRKI
Sbjct: 301 IAIGAYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGRKI 360

Query: 361 SGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKG 420
            GVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPK V GKIVICDRGSSPRVAKG
Sbjct: 361 YGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVAKG 420

Query: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQ 480
           LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSS NPTATIAFQ
Sbjct: 421 LVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIAFQ 480

Query: 481 GTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTE 540
           GTIIGIKPAPVVASFSARGPNGL PEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRKTE
Sbjct: 481 GTIIGIKPAPVVASFSARGPNGLTPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKTE 540

Query: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAS 600
           FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASI DNRRQPMTEESTGK S
Sbjct: 541 FNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASIIDNRRQPMTEESTGKPS 600

Query: 601 TPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPLP 660
           TPYDFGAGHVNLGLAMDPGL+YDITNTDYINFLCSIGYGPKMIQVITRTPV CP KKPLP
Sbjct: 601 TPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKPLP 660

Query: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFS 720
           ENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGP+NSVYR KIEAPKGVTVKVKPSKLVFS
Sbjct: 661 ENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPSNSVYRVKIEAPKGVTVKVKPSKLVFS 720

Query: 721 ATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
            TVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 TTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

BLAST of HG10000583 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 740/774 (95.61%), Postives = 756/774 (97.67%), Query Frame = 0

Query: 1   MAAFLS-ISLSPFFFLLLLILISTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFA 60
           MAA+LS +    FFF LL++   TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFA
Sbjct: 1   MAAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ES KILHVYDTVFHGFSATLTQ QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIV 180

Query: 181 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+AS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360
           DPIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 KISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVA 420
           +ISGVSLYAGAPLNGTM+PLVYPGKSGVLSVSLCM+NSLDPK VAGKIVICDRGSSPRVA
Sbjct: 361 RISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSSPNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIA 480

Query: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRK 540
           FQGTIIGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTDAVGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600
           TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
           LPENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPANSVYRAKIEAPKGVTVKVKPSKLV
Sbjct: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           FSA  KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 FSAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

BLAST of HG10000583 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 737/773 (95.34%), Postives = 753/773 (97.41%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPL-KTFIFRVDRFSKPSVFPTHYHWYTSEFAE 60
           MAA+LS     FFF LL++   TVSADSPL KTFIFRVDRFSKPSVFPTHYHWYTSEFAE
Sbjct: 1   MAAYLS---PLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAE 60

Query: 61  SPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120
           S KILHVYDTVFHGFSATLTQ QVD+IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG
Sbjct: 61  SHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120

Query: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVG 180
           LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTA+NCNRKIVG
Sbjct: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVG 180

Query: 181 ARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 240
           ARFFSKGHEAGSNAAGPIIGINDTIE+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASG
Sbjct: 181 ARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASG 240

Query: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLD 300
           IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD AVNDGVDVISISIGGGDGVSSPYYLD
Sbjct: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 300

Query: 301 PIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRK 360
           PIAIGAYGAASRG+FVSSS GNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR+
Sbjct: 301 PIAIGAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRR 360

Query: 361 ISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAK 420
           ISGVSLYAGAPLN TM+PLVYPGKSGVLSVSLCM+NSLDPK VAGKIVICDRGSSPRVAK
Sbjct: 361 ISGVSLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAK 420

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAF 480
           GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGD+MKAYASSS NPTATIAF
Sbjct: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAF 480

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
           QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT
Sbjct: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKA 600
           EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTAS TDNRRQPMTEESTGKA
Sbjct: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKA 600

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL 660
           STPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYGPKMIQVITRTPVTCPAKKPL
Sbjct: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPL 660

Query: 661 PENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 720
           PENLNYPSIVAVFSSLSKGWSTKSFIRT TNVGPANSVYRAKIEAPKGVTVKVKPSKLVF
Sbjct: 661 PENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 720

Query: 721 SATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           SA +KKQSFVVA+SADNQNLALGDVGAVFGW+SWSDGKHVVRSPLVVTQLEPL
Sbjct: 721 SAAMKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

BLAST of HG10000583 vs. ExPASy TrEMBL
Match: A0A5D3BLE2 (Subtilisin-like protease SBT1.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001210 PE=3 SV=1)

HSP 1 Score: 1436.8 bits (3718), Expect = 0.0e+00
Identity = 714/741 (96.36%), Postives = 726/741 (97.98%), Query Frame = 0

Query: 32  TFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTVFHGFSATLTQDQVDTIGKHPS 91
           TFI R+DRFSKPSVFPTHYHWYTSEF +SP+ILHVYDTVFHGFSATLTQ+QVD+IGKHPS
Sbjct: 2   TFIVRIDRFSKPSVFPTHYHWYTSEFTQSPQILHVYDTVFHGFSATLTQEQVDSIGKHPS 61

Query: 92  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 151
           VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL
Sbjct: 62  VLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNL 121

Query: 152 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRD 211
           GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAG+NAAGPIIGINDTIEYRSPRD
Sbjct: 122 GPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTIEYRSPRD 181

Query: 212 ADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 271
           ADGHGTHTASTAAGRH+FQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF
Sbjct: 182 ADGHGTHTASTAAGRHSFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAF 241

Query: 272 DVAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTN 331
           D AVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS+GVFVSSSAGNDGPNGMSVTN
Sbjct: 242 DAAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAASKGVFVSSSAGNDGPNGMSVTN 301

Query: 332 LAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 391
           LAPWVTTVGAGTIDRNFP+VVTLGNGRKI GVSLYAGAPLNGTMYPLVYPGKSGVLSVSL
Sbjct: 302 LAPWVTTVGAGTIDRNFPSVVTLGNGRKIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSL 361

Query: 392 CMENSLDPKAVAGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 451
           CMENSLDPK V GKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP
Sbjct: 362 CMENSLDPKVVTGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLP 421

Query: 452 ACAVGSDEGDAMKAYASSSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPD 511
           ACAVGSDEGDAMKAYASSS NPTATIAFQGTIIGIKPAPVVASFSARGPNGL PEILKPD
Sbjct: 422 ACAVGSDEGDAMKAYASSSTNPTATIAFQGTIIGIKPAPVVASFSARGPNGLTPEILKPD 481

Query: 512 LIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 571
           +IAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA
Sbjct: 482 IIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPA 541

Query: 572 ALRSAMMTTASITDNRRQPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYIN 631
           ALRSAMMTTASI DNRRQPMTEESTGK STPYDFGAGHVNLGLAMDPGL+YDITNTDYIN
Sbjct: 542 ALRSAMMTTASIIDNRRQPMTEESTGKPSTPYDFGAGHVNLGLAMDPGLIYDITNTDYIN 601

Query: 632 FLCSIGYGPKMIQVITRTPVTCPAKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNV 691
           FLCSIGYGPKMIQVITRTPV CP KKPLPENLNYPSIVAVFSSLSKGWSTKSFIRT TNV
Sbjct: 602 FLCSIGYGPKMIQVITRTPVRCPTKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTVTNV 661

Query: 692 GPANSVYRAKIEAPKGVTVKVKPSKLVFSATVKKQSFVVAISADNQNLALGDVGAVFGWL 751
           GP+NSVYR KIEAPKGVTVKVKPSKLVFS TVKKQSFVVAISADNQNLALGDVGAVFGWL
Sbjct: 662 GPSNSVYRVKIEAPKGVTVKVKPSKLVFSTTVKKQSFVVAISADNQNLALGDVGAVFGWL 721

Query: 752 SWSDGKHVVRSPLVVTQLEPL 773
           SWSDGKHVVRSPLVVTQLEPL
Sbjct: 722 SWSDGKHVVRSPLVVTQLEPL 742

BLAST of HG10000583 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 567/764 (74.21%), Postives = 664/764 (86.91%), Query Frame = 0

Query: 15  LLLLILIS----TVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAESPKILHVYDTV 74
           ++LL+ +S    + +A    KTFIFR+D  S PS+FPTHYHWY++EFAE  +I+HVY TV
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVYHTV 65

Query: 75  FHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDV 134
           FHGFSA +T D+ D +  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYGSDV
Sbjct: 66  FHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDV 125

Query: 135 IIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSKGHEAG 194
           IIGVFDTGI PERRSFSD+NLGPIP+RW+GVCE+G +F+ +NCNRKI+GARFF+KG +  
Sbjct: 126 IIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQ-- 185

Query: 195 SNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARL 254
              A  I GIN T+E+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPKAR+
Sbjct: 186 ---AAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARI 245

Query: 255 AVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIAIGAYGAAS 314
           A YKVCWK+SGC DSDILAAFD AV DGVDVISISIGGGDG++SPYYLDPIAIG+YGAAS
Sbjct: 246 AAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 305

Query: 315 RGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSLYAGAP 374
           +G+FVSSSAGN+GPNGMSVTNLAPWVTTVGA TIDRNFPA   LG+G ++ GVSLYAG P
Sbjct: 306 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVP 365

Query: 375 LNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGLVVKKAGGVG 434
           LNG M+P+VYPGKSG+ S SLCMEN+LDPK V GKIVICDRGSSPRVAKGLVVKKAGGVG
Sbjct: 366 LNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVG 425

Query: 435 MILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQGTIIGIKPAP 494
           MILANG SNGEGLVGDAHL+PACAVGS+EGD +KAYASS PNP A+I F+GTI+GIKPAP
Sbjct: 426 MILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAP 485

Query: 495 VVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNILSGTSMA 554
           V+ASFS RGPNGL+PEILKPDLIAPGVNILAAWTDAVGPTGL  D RKTEFNILSGTSMA
Sbjct: 486 VIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMA 545

Query: 555 CPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKASTPYDFGAGHV 614
           CPHVSGAAALLKSAHPDWSPA +RSAMMTT ++ DN  + + +ESTGK++TPYD+G+GH+
Sbjct: 546 CPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHL 605

Query: 615 NLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCP-AKKPLPENLNYPSIV 674
           NLG AM+PGLVYDITN DYI FLCSIGYGPK IQVITRTPV CP  +KP P NLNYPSI 
Sbjct: 606 NLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSIT 665

Query: 675 AVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSATVKKQSFV 734
           AVF +  +G  +K+ IRTATNVG A +VYRA+IE+P+GVTV VKP +LVF++ VK++S+ 
Sbjct: 666 AVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYA 725

Query: 735 VAISADNQNLALGDVGAVFGWLSWSD-GKHVVRSPLVVTQLEPL 773
           V ++ + +N+ LG+ GAVFG ++W D GKHVVRSP+VVTQ++ L
Sbjct: 726 VTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of HG10000583 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 852.4 bits (2201), Expect = 2.8e-247
Identity = 452/779 (58.02%), Postives = 548/779 (70.35%), Query Frame = 0

Query: 12  FFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA----ESPKILHVY 71
           FFFLL L   S+ ++ S   T+I  VD  +KPS+FPTH+HWYTS  A      P I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 72  DTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 131
           DTVFHGFSA LT      +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 132 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARFFSK 191
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 192 GHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 251
           G+EA +        +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 252 PKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIAIGA 311
           PKARLA YKVCW NSGC+DSDILAAFD AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 312 YGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISGVSL 371
           +GA  RG+FVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V LGNG+ ISGVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 372 YAGAPLN-GTMYPLVYPGK---SGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGL 431
           Y G  L+ G MYPLVY G        S SLC+E SLDP  V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 432 VVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYAS------SSPNPTA 491
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 492 TIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFD 551
           TI F+GT +GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 552 TRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEES 611
            R+TEFNILSGTSMACPHVSG AALLK+AHPDWSPAA+RSA++TTA   DN  +PM +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 612 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPA 671
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DYINFLC+  Y    I  ITR    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 672 KKPLPE--NLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVK 731
            +      NLNYPS   VF    +   +  FIRT TNVG ++SVY  KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 732 PSKLVFSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 773
           P KL F    +K SFVV +      L+ G      G + WSDGK  V SPLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of HG10000583 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 734.9 bits (1896), Expect = 6.4e-212
Identity = 393/768 (51.17%), Postives = 523/768 (68.10%), Query Frame = 0

Query: 7   ISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTS---EFAESPKI 66
           +S + FF LL L      S+ S   T+I  + +   PS F  H +WY S     ++S ++
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAEL 65

Query: 67  LHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGLWS 126
           L+ Y+   HGFS  LTQ++ D++   P V++V  + R +LHTTR+P FLGL  +   L+ 
Sbjct: 66  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFP 125

Query: 127 DSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVGARF 186
           ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG CE GT FTA  CNRK++GARF
Sbjct: 126 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 185

Query: 187 FSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAK 246
           F++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG A+
Sbjct: 186 FARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTAR 245

Query: 247 GVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLDPIA 306
           G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    S YY D +A
Sbjct: 246 GMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDGVA 305

Query: 307 IGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRKISG 366
           IGA+ A  RG+ VS SAGN GP+  S++N+APW+TTVGAGT+DR+FPA+  LGNG+  +G
Sbjct: 306 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 365

Query: 367 VSLYAGAPLNGTMYPLVYPGK-SGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAKGL 426
           VSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV KG 
Sbjct: 366 VSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGD 425

Query: 427 VVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAFQG 486
           VVK AGGVGMILAN  +NGE LV DAHLLPA  VG   GD ++ Y ++ PNPTA+I+  G
Sbjct: 426 VVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILG 485

Query: 487 TIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEF 546
           T++G+KP+PVVA+FS+RGPN + P ILKPDLIAPGVNILAAWT A GPTGL  D+R+ EF
Sbjct: 486 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 545

Query: 547 NILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKAST 606
           NI+SGTSM+CPHVSG AALLKS HP+WSPAA+RSA+MTTA  T    +P+ + +TGK ST
Sbjct: 546 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 605

Query: 607 PYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTC-PAKKPLP 666
           P+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y    I+ ++R   TC P+K    
Sbjct: 606 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 665

Query: 667 ENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEA-PKGVTVKVKPSKLVF 726
            +LNYPS    F+    G     + RT T+VG A + Y  K+ +   GV + V+P+ L F
Sbjct: 666 ADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNF 725

Query: 727 SATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVT 768
               +K+S+ V  + D+   +  +    FG + WSDGKHVV SP+ ++
Sbjct: 726 KEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

BLAST of HG10000583 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 716.1 bits (1847), Expect = 3.1e-206
Identity = 383/766 (50.00%), Postives = 508/766 (66.32%), Query Frame = 0

Query: 1   MAAFLSISLSPFFFLLLLILISTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFAES 60
           MA+  S S S       L L+   +A    KT+I RV+   KP  F TH+ WYTS+    
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK---KTYIIRVNHSDKPESFLTHHDWYTSQLNSE 60

Query: 61  PKILHVYDTVFHGFSATLTQDQVDT-IGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 120
             +L+ Y T FHGFSA L   + D+ +    S+L +FED    LHTTR+P+FLGL ++ G
Sbjct: 61  SSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 120

Query: 121 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIVG 180
           +       + VIIGV DTG+ PE RSF D ++  IP +WKG CE+G+ F +K CN+K++G
Sbjct: 121 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 180

Query: 181 ARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 240
           AR FSKG +  S       G +   E  SPRD DGHGTHT++TAAG     AS  GYA+G
Sbjct: 181 ARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 240

Query: 241 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDGVSSPYYLD 300
            A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S+PYY D
Sbjct: 241 TARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYYRD 300

Query: 301 PIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRK 360
            IAIGA+ A  RGVFVS SAGN GP   SV N+APWV TVGAGT+DR+FPA   LGNG++
Sbjct: 301 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 360

Query: 361 ISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVAK 420
           ++GVSLY+G  +      LVY  K    S +LC+  SLD   V GKIV+CDRG + RV K
Sbjct: 361 LTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEK 420

Query: 421 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSPNPTATIAF 480
           G VV+ AGG+GMI+AN  ++GE LV D+HLLPA AVG   GD ++ Y  S   PTA + F
Sbjct: 421 GAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVF 480

Query: 481 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKT 540
           +GT++ +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+DA+GPTGLD D+R+T
Sbjct: 481 KGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRT 540

Query: 541 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGKA 600
           +FNI+SGTSM+CPH+SG A LLK+AHP+WSP+A++SA+MTTA + DN   P+ + +    
Sbjct: 541 QFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSL 600

Query: 601 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYG-PKMIQVITRTPVTCPAKKP 660
           S PY  G+GHV+   A+ PGLVYDI+  +YI FLCS+ Y    ++ ++ R  V C  K  
Sbjct: 601 SNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS 660

Query: 661 LPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIEAPKGVTVKVKPSKLV 720
            P  LNYPS    FS L  G     + R  TNVG A+SVY+  +     V + VKPSKL 
Sbjct: 661 DPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLS 720

Query: 721 FSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPL 765
           F +  +K+ + V      + +++ +  A FG ++WS+ +H VRSP+
Sbjct: 721 FKSVGEKKRYTVTF-VSKKGVSMTN-KAEFGSITWSNPQHEVRSPV 747

BLAST of HG10000583 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 694.9 bits (1792), Expect = 7.4e-200
Identity = 384/789 (48.67%), Postives = 507/789 (64.26%), Query Frame = 0

Query: 11  PFFFLLLLILISTVSAD-----SPLKTFIFRVDRFSKPSVFPTHYHWYTS---------- 70
           PF F++L I +  + A+     S  KT++  +D+ + P  +  H  WY+S          
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 71  --EFAESPKILHVYDTVFHGFSATLTQDQVDTIGKHPSVLAVFEDRRRQLHTTRSPQFLG 130
             E   + +IL+ Y T FHG +A LTQ++ + + +   V+AV  + R +LHTTRSP FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 131 LRNQRG--LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAK 190
           L  Q    +W++     DV++GV DTGI PE  SF+D  + P+P  W+G CETG +F  +
Sbjct: 130 LERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKR 189

Query: 191 NCNRKIVGARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQA 250
           NCNRKIVGAR F +G+EA +        I++ +EY+SPRD DGHGTHTA+T AG     A
Sbjct: 190 NCNRKIVGARVFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGA 249

Query: 251 SLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDVAVNDGVDVISISIGGGDG 310
           +L G+A G A+G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GGG  
Sbjct: 250 NLFGFAYGTARGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGGG-- 309

Query: 311 VSSPYYLDPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAV 370
             S Y  D ++I  +GA   GVFVS SAGN GP+ +S+TN++PW+TTVGA T+DR+FPA 
Sbjct: 310 -VSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPAT 369

Query: 371 VTLGNGRKISGVSLYAGAPL--NGTMYPLVYPGK--SGVLSVSLCMENSLDPKAVAGKIV 430
           V +G  R   GVSLY G  +      YPLVY G+  S     S C++ +LD + VAGKIV
Sbjct: 370 VKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIV 429

Query: 431 ICDRGSSPRVAKGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYA 490
           ICDRG +PRV KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA
Sbjct: 430 ICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYA 489

Query: 491 SSSPNPTATIAFQGTIIGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAV 550
            +S   TA++   GT IGIKP+PVVA+FS+RGPN L+ EILKPDL+APGVNILAAWT  +
Sbjct: 490 MTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDM 549

Query: 551 GPTGLDFDTRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNR 610
            P+ L  D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSPAA++SA+MTTA + DN 
Sbjct: 550 APSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNM 609

Query: 611 RQPMTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVIT 670
            +P+T+ S    S+PYD GAGH++   A DPGLVYDI   +Y  FLC+    P  ++V T
Sbjct: 610 FKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFT 669

Query: 671 R-TPVTCP---AKKPLPENLNYPSIVAVFSSLSKGWSTKSFIRTATNVGPANSVYRAKIE 730
           + +  TC    AK   P NLNYP+I A+F   +      +  RT TNVGP  S Y+  + 
Sbjct: 670 KHSNRTCKHTLAKN--PGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVS 729

Query: 731 APKGVTVKVKPSKLVFSATVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSP 773
             KG +V V+P  L F++  +K S+ V      +          FG L W    H VRSP
Sbjct: 730 PFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSP 780