HG10000577 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000577 vs. NCBI nr
Match: XP_038902519.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 802.0 bits (2070), Expect = 2.8e-228
Identity = 420/494 (85.02%), Postives = 432/494 (87.45%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+KL PSTT LKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLKLRPSTTALKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIACD FPTW ILLIGSLEGLIGYG QWLVVSQRISPLPYWQMCIFLCLGG
Sbjct: 61  VGKAFGLLAGIACDRFPTWAILLIGSLEGLIGYGVQWLVVSQRISPLPYWQMCIFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSS+PS FLL+L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSNPSPFLLMLA 180

Query: 181 FVPFAVCLFAMFFLA-------------------------------------DFIKNSGR 240
           FVPFAVCLFAMFFL                                      DFIKNSGR
Sbjct: 181 FVPFAVCLFAMFFLREIPPPPSTTAADTQQESDYFSVFNALAVVVAVYLLCFDFIKNSGR 240

Query: 241 LVSQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA 300
           L+SQLFS GLL LLGSPLVIPIY+FFKSW+SN+DRSD+EARIEEPLLKEE V   VKEEA
Sbjct: 241 LISQLFSFGLLFLLGSPLVIPIYSFFKSWNSNRDRSDLEARIEEPLLKEEVVTEAVKEEA 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
            EIA+EEQR PVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 GEIAKEEQRLPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSL SIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGY+LMAMAMPGSL
Sbjct: 361 VSMFVSLISIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGSVIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSVIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. NCBI nr
Match: XP_022970978.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 775.4 bits (2001), Expect = 2.8e-220
Identity = 406/494 (82.19%), Postives = 431/494 (87.25%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS KLPPSTTVLKWLGFVSAVW+QAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWIQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIA D FPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMC FLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 FVPFAVCLFAMFFLA----------------------------------DFIKNSGRLVS 240
            VPFAVCLFAMFFL                                   DFIKNSG++VS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKE---EA 300
           QLFS+GLLILLGSPLVIPIY+FFKSW+SNQ + D+EA+IEEPLLKEEA+AA  +    + 
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSFFKSWNSNQTQLDLEAKIEEPLLKEEALAAAEQSTVAKE 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           EEIA EE+  PVIGEEHTIFEA+KT+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 EEIA-EERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILMT+GYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. NCBI nr
Match: XP_022985694.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 405/493 (82.15%), Postives = 428/493 (86.82%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+KLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSD LKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDVLKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIACD FPTWVILLIGSLEGL+GYGAQWLVVSQRISPLPYWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGIACDRFPTWVILLIGSLEGLLGYGAQWLVVSQRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS+DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPSSFLVMLS 180

Query: 181 FVPFAVCLFAMFFLA-----------------------------------DFIKNSGRLV 240
            VPFAVCLFAMFFL                                    DFIKN G +V
Sbjct: 181 VVPFAVCLFAMFFLREIPPPTTAADTQEESNFFAIFNALAVIVAVYLLCFDFIKNFGTVV 240

Query: 241 SQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEA-VAAPVKEEAE 300
           SQLFS+GLLILLGSPL+IP+Y+FFK+ HSN  +SD+E + EEPLLKEEA VAAPVK   E
Sbjct: 241 SQLFSVGLLILLGSPLIIPMYSFFKNCHSNSAQSDLETQTEEPLLKEEATVAAPVK---E 300

Query: 301 EIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADV 360
           EI EEEQ+ PVIGEEHTI +AVKTIDFWVLF+SFLCGVGTGLAVMNNMGQIGLALGYADV
Sbjct: 301 EITEEEQKRPVIGEEHTIIQAVKTIDFWVLFISFLCGVGTGLAVMNNMGQIGLALGYADV 360

Query: 361 SMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSLY 420
           SMFVSL SIWGFFGRILSG+LSEHFLKK GTPRPLWNAASQILMT+GYILMAMAMPGSLY
Sbjct: 361 SMFVSLISIWGFFGRILSGSLSEHFLKKLGTPRPLWNAASQILMTIGYILMAMAMPGSLY 420

Query: 421 IGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME 458
           IGSVIVGICYG+RLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME
Sbjct: 421 IGSVIVGICYGIRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME 480

BLAST of HG10000577 vs. NCBI nr
Match: XP_008457674.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 407/494 (82.39%), Postives = 427/494 (86.44%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+ LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAG+A D FPTWVILLIGS+EGL+GYGAQWLVVS+RISPL YWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLVVSRRISPLSYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLL+L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 FVPFAVCLFAMFFLA-------------------------------------DFIKNSGR 240
            VPFAVCLFAMFFL                                      DFIKNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LVSQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA 300
           L+SQL+S+GLLILLGSPL+IPIY+FFKSW+S + R D    +EEPL+KEEAV   VKEE 
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLD----LEEPLVKEEAVTGAVKEET 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
            EIA  EQRAPVIGEEHTIFEAV+TIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 GEIAMIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGY+LMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGSVIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSVIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. NCBI nr
Match: XP_023532459.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.1 bits (1995), Expect = 1.4e-219
Identity = 404/494 (81.78%), Postives = 427/494 (86.44%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS KLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIA D FPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMC FLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 FVPFAVCLFAMFFLA----------------------------------DFIKNSGRLVS 240
            VPFAVCLFAMFFL                                   DFIKNSG++VS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA--- 300
           QLFS+GLLILLGSPLVIPIY+ FKSW+SNQ + D+EA+IEEPLLKEEA AA  ++     
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLLKEEAAAAAAEQSTVVK 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           EE   EE+  PVIGEEHTIFEA+KT+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 EEEITEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILMT+GYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-50
Identity = 149/492 (30.28%), Postives = 249/492 (50.61%), Query Frame = 0

Query: 6   LPPSTTVL----KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDV 65
           LPP  T+     KW   V+A+W+QA +G N+ FS YS  LKS++ ++Q++LN L+VA D+
Sbjct: 31  LPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDL 90

Query: 66  GKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLC--LG 125
           GKAFG  +GIA  +FP  V+L   +  G +GYG QWLV++  I+ LPY    +FLC  L 
Sbjct: 91  GKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIIT-LPY--SLVFLCCLLA 150

Query: 126 GNSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLL 185
           G S  W NTA  + CIR+F  NR     +   + G+S A+++    A+  S  + +LLL 
Sbjct: 151 GLSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLN 210

Query: 186 VFVPFAVCLFAMF-------------------------------FLADF---IKNSGRLV 245
             VP  V   A++                                +  F   + +S    
Sbjct: 211 SLVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS 270

Query: 246 SQLFSIGLLILLGSPLVIPIYTFFKSWH--------SNQDRSDMEARIEEPLLKEEAVAA 305
           ++L  IG ++LL  PL  P+  + + +         +++    +   I+E  LK +  + 
Sbjct: 271 ARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDE--LKNQKTSV 330

Query: 306 PVKEEAEEIAEEEQRAPV-IGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIG 365
             K   E +   ++   V +G+EH+    +  ++FW+ ++++ CG   GL   NN+GQI 
Sbjct: 331 SSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 390

Query: 366 LALGYADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMA 425
            +LG    ++ V++ S + FFGR+LS    +   K+    R  W A + +   + + L+A
Sbjct: 391 QSLGQNSTTL-VTIYSSFSFFGRLLSAA-PDFMHKRFRLTRTGWFAIALLPTPIAFFLLA 450

Query: 426 M--AMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSG 447
           +  +   +L   + ++G+  G   +  V   S+LFG    G+ +NILI N+PIGS L+ G
Sbjct: 451 VSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY-G 510

BLAST of HG10000577 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 9.2e-09
Identity = 111/493 (22.52%), Postives = 193/493 (39.15%), Query Frame = 0

Query: 28  SGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGL-LAGIACDWFPTWVILLIGS 87
           +G+   FS Y   L + +N TQL++N +S+A  +     + LAG  CD +    + L   
Sbjct: 94  AGSITAFSLYGPLLLTRLNYTQLRVNEVSIAAGISMYLPVSLAGYLCDRYSPSPLTLFAG 153

Query: 88  LEGLIGYGAQWLVVSQRISP------LPYWQMCIFLCLGGNSTTWMNTAVLVTCIRNF-- 147
           +   +GY     V      P       P+W M +     G +T  M  A + TC +NF  
Sbjct: 154 IAFGLGYSLAAFVYKSGPPPDAGGNGWPFWVMVVAFIAIGVATCSMYLAAVTTCAKNFGR 213

Query: 148 RKNRGPVSGILKGYVGLSTAIFTD-----LCFALFSSDPSSFLLLLVFVPFAVCLFAMFF 207
            K++G +  +     GLS    +      LC  L  S+     +   F+  A+ L  +  
Sbjct: 214 GKHKGIILAVPIAAFGLSGMWQSQVGTYFLCERLKDSNCGDVDVYKYFLFLAILLLTIGV 273

Query: 208 LADFI---------KNSGRLVSQLFSIGLL----ILLGSPLVIPIYTFFKSWHSNQDRSD 267
           +  F          K     V +L   GLL           V   Y  F   H +    D
Sbjct: 274 IGTFALRIVDDEEEKYIDEAVEELERSGLLAESEFFRPRSEVQAAYGTFSDDHEDNGSVD 333

Query: 268 MEARIEEPLLKEEAVAAPVKEEAEEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLC 327
            ++      + EE   A  +E+  E  E  ++  ++  E  IF A  T+  W L + F  
Sbjct: 334 DQS----VTISEELREAARREKEREEEERRKKNWLLNYETRIFLADHTM--WWLALGFFL 393

Query: 328 GVGTGLAVMNNMGQIGLALGYADV-----------SMFVSLTSIWGFFGRILSGTLSE-- 387
             G G A +NN+G I   L                S  V++ ++     R+L+G+LS+  
Sbjct: 394 VTGPGEAYLNNLGTIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTSTIARLLTGSLSDLF 453

Query: 388 ------HFLKKAGTPRP-------------LWNAASQILMTVGYILMAMAMPGS----LY 447
                 HF     T  P              +   S +L+++G++L+A  +P       +
Sbjct: 454 APTARRHFTVDQETAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLLASPLPTHHPELSH 513

Query: 448 IGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME 458
           + + +VG+ YG   S+     S ++G++ +G  + I+ +    G+ ++     G +Y   
Sbjct: 514 LTTALVGLGYGSIFSLVPIIISVVWGVENFGTNWGIVAMFPAAGAAMW-----GVIYSRA 573

BLAST of HG10000577 vs. ExPASy TrEMBL
Match: A0A6J1I5G9 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469786 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.3e-220
Identity = 406/494 (82.19%), Postives = 431/494 (87.25%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS KLPPSTTVLKWLGFVSAVW+QAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWIQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIA D FPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMC FLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 FVPFAVCLFAMFFLA----------------------------------DFIKNSGRLVS 240
            VPFAVCLFAMFFL                                   DFIKNSG++VS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKE---EA 300
           QLFS+GLLILLGSPLVIPIY+FFKSW+SNQ + D+EA+IEEPLLKEEA+AA  +    + 
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSFFKSWNSNQTQLDLEAKIEEPLLKEEALAAAEQSTVAKE 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           EEIA EE+  PVIGEEHTIFEA+KT+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 EEIA-EERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILMT+GYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. ExPASy TrEMBL
Match: A0A6J1JE03 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483674 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.1e-220
Identity = 405/493 (82.15%), Postives = 428/493 (86.82%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+KLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSD LKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDVLKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIACD FPTWVILLIGSLEGL+GYGAQWLVVSQRISPLPYWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGIACDRFPTWVILLIGSLEGLLGYGAQWLVVSQRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS+DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPSSFLVMLS 180

Query: 181 FVPFAVCLFAMFFLA-----------------------------------DFIKNSGRLV 240
            VPFAVCLFAMFFL                                    DFIKN G +V
Sbjct: 181 VVPFAVCLFAMFFLREIPPPTTAADTQEESNFFAIFNALAVIVAVYLLCFDFIKNFGTVV 240

Query: 241 SQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEA-VAAPVKEEAE 300
           SQLFS+GLLILLGSPL+IP+Y+FFK+ HSN  +SD+E + EEPLLKEEA VAAPVK   E
Sbjct: 241 SQLFSVGLLILLGSPLIIPMYSFFKNCHSNSAQSDLETQTEEPLLKEEATVAAPVK---E 300

Query: 301 EIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADV 360
           EI EEEQ+ PVIGEEHTI +AVKTIDFWVLF+SFLCGVGTGLAVMNNMGQIGLALGYADV
Sbjct: 301 EITEEEQKRPVIGEEHTIIQAVKTIDFWVLFISFLCGVGTGLAVMNNMGQIGLALGYADV 360

Query: 361 SMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSLY 420
           SMFVSL SIWGFFGRILSG+LSEHFLKK GTPRPLWNAASQILMT+GYILMAMAMPGSLY
Sbjct: 361 SMFVSLISIWGFFGRILSGSLSEHFLKKLGTPRPLWNAASQILMTIGYILMAMAMPGSLY 420

Query: 421 IGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME 458
           IGSVIVGICYG+RLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME
Sbjct: 421 IGSVIVGICYGIRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDME 480

BLAST of HG10000577 vs. ExPASy TrEMBL
Match: A0A1S3C607 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497317 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.1e-220
Identity = 407/494 (82.39%), Postives = 427/494 (86.44%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+ LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAG+A D FPTWVILLIGS+EGL+GYGAQWLVVS+RISPL YWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLVVSRRISPLSYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLL+L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 FVPFAVCLFAMFFLA-------------------------------------DFIKNSGR 240
            VPFAVCLFAMFFL                                      DFIKNSGR
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 LVSQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA 300
           L+SQL+S+GLLILLGSPL+IPIY+FFKSW+S + R D    +EEPL+KEEAV   VKEE 
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLD----LEEPLVKEEAVTGAVKEET 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
            EIA  EQRAPVIGEEHTIFEAV+TIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 GEIAMIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGY+LMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGSVIVGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSVIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. ExPASy TrEMBL
Match: A0A6J1G8P6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451839 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.7e-220
Identity = 404/494 (81.78%), Postives = 428/494 (86.64%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS KLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIA D FPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMC FLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL++L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 FVPFAVCLFAMFFLA----------------------------------DFIKNSGRLVS 240
            VPFAVCLFAMFFL                                   DFIKNSG++VS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA--- 300
           QLFS+GLLILLGSPLVIPIY+F KSW+SNQ + D+EA+IEEPLLKEEA AA V++     
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSFIKSWNSNQTQLDLEAKIEEPLLKEEATAAAVEQSTVVK 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           EE   EE+  PVIGEEHTIFEA+KT+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 EEEITEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILMT+GYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. ExPASy TrEMBL
Match: A0A0A0LLL4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264630 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.5e-219
Identity = 406/494 (82.19%), Postives = 425/494 (86.03%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MGS+ LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD
Sbjct: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFGLLAGIA D FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMCIFLC+GG
Sbjct: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLL+L 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 FVPFAVCLFAMFFLA-------------------------------------DFIKNSGR 240
            VP AVCLFAMFFL                                      DF+KNSGR
Sbjct: 181 LVPLAVCLFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240

Query: 241 LVSQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEA 300
           L+SQL+SIGLLILLGSPL+IPIY+FFKSW+S + R D    +EEPL+KEE V   VKEEA
Sbjct: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLD----LEEPLVKEEVVTGAVKEEA 300

Query: 301 EEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
            E A  EQRAPVIGEEHTIFEAV+TIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 GETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILSGT+SEHFLKKAGTPRPLWNAASQILMTVGY+LMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMPGSL 420

Query: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 458
           YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

BLAST of HG10000577 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 638.3 bits (1645), Expect = 4.9e-183
Identity = 339/497 (68.21%), Postives = 388/497 (78.07%), Query Frame = 0

Query: 1   MGSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60
           MG  +   S++ LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLMNL QL+LNNLSVAKD
Sbjct: 1   MGFQRSISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKD 60

Query: 61  VGKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGG 120
           VGKAFG+LAG+A D  PT VILLIG  EGL+GYG QWLVVS+ I P+PYWQMCIFLC+GG
Sbjct: 61  VGKAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLV 180
           NSTTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLC ALFS+DP+SFL+LL 
Sbjct: 121 NSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLA 180

Query: 181 FVPFAVCLFAMFFLA-----------------------------------DFIKNSGRLV 240
            VPFAVCL A+FFL                                    D I     + 
Sbjct: 181 VVPFAVCLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVF 240

Query: 241 SQLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAP-----VK 300
           S  F+  LL LL SP+ IP ++F KS +   ++ D+E RI+EPLL+ E  AA      V 
Sbjct: 241 SVAFASILLFLLASPIAIPFHSFIKSLNYG-EQDDLEGRIQEPLLRSEIAAAEKEVIVVA 300

Query: 301 EEAEEIAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
             A E  E++++ PV+GE+HTI EAV T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 AVAAEEEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMP 420
           Y +VS+FVS+TSIWGFFGRILSGTLSE+FLKKAGTPRPLWNAASQILM VGYILMA+A+P
Sbjct: 361 YTNVSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVP 420

Query: 421 GSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 458
            SLYIGS++VG+CYGVRL++TVPTASELFGLKYYGLIYNIL+LNLP+GSFLFSGLLAGFL
Sbjct: 421 NSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFL 480

BLAST of HG10000577 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 625.5 bits (1612), Expect = 3.3e-179
Identity = 336/516 (65.12%), Postives = 386/516 (74.81%), Query Frame = 0

Query: 2   GSMKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDV 61
           G+     S++ LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLMNLTQL+LN+LSVAKDV
Sbjct: 4   GTSSSSSSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDV 63

Query: 62  GKAFGLLAGIACDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGGN 121
           GKAFG+LAG+A D   T VILLIGS EGL+GYG QWLVVS+ I P+PYWQMC+FLC+GGN
Sbjct: 64  GKAFGILAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGN 123

Query: 122 STTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLVF 181
           STTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLC ALFSSDP+SFL+LL  
Sbjct: 124 STTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSV 183

Query: 182 VPFAVCLFAMFFLA-----------------------------------DFIKNSGRLVS 241
           VPFAVCL A+FFL                                    D I       S
Sbjct: 184 VPFAVCLTAVFFLREIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFS 243

Query: 242 QLFSIGLLILLGSPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLK-------EEAVAA-- 301
             F+  LLILL SP+ +P + F +S     D  D+E RI+EPLL+       EE +    
Sbjct: 244 IAFASILLILLASPVAVPFHAFIRS--KVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAA 303

Query: 302 ------------PVKEEAEE----IAEEEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCG 361
                       P+  E EE    I   E++ PV+GEEHTI EA+ T+DFWVLFVSFLCG
Sbjct: 304 AAADNELPPSLKPLSNEEEENHGTIVTTEKKRPVLGEEHTIMEAMLTVDFWVLFVSFLCG 363

Query: 362 VGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWN 421
           VGTGLAVMNNMGQIGLALGY DVS+FVS+TSIWGFFGRILSGT+SEHF+KKAGTPRPLWN
Sbjct: 364 VGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLWN 423

Query: 422 AASQILMTVGYILMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNIL 458
           AA+QI+M VGY+LMA+A+PGSLYIGS++VG+CYGVRL++TVPTASELFGLKYYGLIYNIL
Sbjct: 424 AAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNIL 483

BLAST of HG10000577 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 9.3e-81
Identity = 179/511 (35.03%), Postives = 276/511 (54.01%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIAC 73
           +WL FV+A+W+Q+ +G  Y F + S  +KS +N  Q +L+ L VAKD+G + G +AG   
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 74  DWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGGNSTTWMNTAVLVT 133
           +  P W  LL+G+++ LIGYG  WL+V+ R   LP W MC+ + +G N  T+ NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 134 CIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLVFVPFAVCLFAMFF 193
            ++NF K+RGPV GILKG+ GL  AI + +   + SS+P+S +L++   P  V +  MFF
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFF 195

Query: 194 -------------------------------------LADFIKNSGRLVSQLFSIGLLIL 253
                                                + D +  S  +++ +F+I L ++
Sbjct: 196 IRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVIT-VFTIVLFVI 255

Query: 254 LGSPLVIPIYT-FFKSWHSNQD---------RSDMEARIEEPLL---------KEEAVAA 313
           L  P+++PI T FF   +   D         R D E  ++ P L          ++    
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLL 315

Query: 314 PVKEEAEEIAE----------------EEQRAPVIGEEHTIFEAVKTIDFWVLFVSFLCG 373
           P  E  + IA                   +R P  GE+ T+ +A+   DFW++F S L G
Sbjct: 316 PASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLG 375

Query: 374 VGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWN 433
            G+GL V++N+GQ+  +LGY +  + VS+ SIW F GRI  G  SE  ++    PRP+  
Sbjct: 376 SGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPVAM 435

Query: 434 AASQILMTVGYILMAMAMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNIL 453
           A +Q++M+VG+I  A   PG++YIG++++G+ YG   ++   TASELFGLK +G +YN L
Sbjct: 436 AVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYNFL 495

BLAST of HG10000577 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.4e-75
Identity = 174/488 (35.66%), Postives = 269/488 (55.12%), Query Frame = 0

Query: 15  WLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIACD 74
           W+G  +A WVQ  +G+  TF  YS ALKS++  +Q Q+  L VA D+G+  GLL G A +
Sbjct: 14  WVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDLGENMGLLPGYASN 73

Query: 75  WFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGGNSTTWMNTAVLVTC 134
             P W +LLIG+    +G+G  WL VSQ +  LP+W + + L L  NS +W  TA LVT 
Sbjct: 74  KLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATNSNSWFGTASLVTN 133

Query: 135 IRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLVFVPFAVCLFAMFFL 194
           +RNF  +RGPV+G+LKGY+G+S A FT L   +        LL L      +CL  M+F+
Sbjct: 134 MRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTVGIPVICLTVMYFI 193

Query: 195 ADFIKNSGR--------------------------LVSQLFSIG----------LLILLG 254
              I  +G                           ++S++F +           +++LL 
Sbjct: 194 RPCIPATGEDPSEPMYFAFLLVTSILFAAYLVVTTVLSEVFILPSILKYVLVAIMVLLLL 253

Query: 255 SPLVIPI-YTFFKS------WHSNQDRSDMEARIEEPLLKEEAVAA---PVKEEAEE--- 314
           SPL +PI  T F+S        S+ + +  E   EEPLL     A+   P+ E  +E   
Sbjct: 254 SPLAVPIKMTLFRSNAKSSPLGSSDNLAKEEGTHEEPLLTPSTSASNLGPIFEGDDESDM 313

Query: 315 ---IAEEE-----QRAPVIGEEHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGL 374
              +AE E     +R P  GE+    +     DFW+L+  +  G+G+G+ V NN+ QIG 
Sbjct: 314 EILLAEAEGAVKKKRKPRRGEDFKFGQVFVKADFWLLWFVYFLGMGSGVTVSNNLAQIGF 373

Query: 375 ALGYADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAM 434
           A G  D ++ + L S + F GR+ SG +SEHF++    PR LW  A+Q++M   ++L AM
Sbjct: 374 AFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTLPRTLWMGAAQLVMVFTFLLFAM 433

Query: 435 AMPGSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLA 446
           A+  ++Y+ + ++GIC G +  +++ T SELFGL+++G+ +N ++L  P+G+ +FS +LA
Sbjct: 434 AIDHTIYVATALIGICMGFQF-LSIATISELFGLRHFGINFNFILLGNPLGATIFSAILA 493

BLAST of HG10000577 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 7.6e-75
Identity = 168/480 (35.00%), Postives = 274/480 (57.08%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTF-SNYSDALKSLMNLTQLQLNNLSVAKDVGKAFGLLAGIA 73
           +WL FV A+WVQ+++G  Y F  + S A+K+ +   Q Q+  L VAK++G A G ++G  
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 74  CDWFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCIFLCLGGNSTTWMNTAVLV 133
            +  P+WV+LL+G+ + L GYG  WLVV+ ++  LP W + + + +G N  T+ NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 134 TCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLLLVFVPFAVCLFAMF 193
           +CI NF ++RGPV GILKG+ GLS AI T + + +F+    S ++L+V +   V + A+ 
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQV-YLMFNPSHDSSVILMVALGPPVVVLALL 195

Query: 194 FLADFIKNSGR-----------------LVSQLFSIGLLIL-----------------LG 253
           F+   ++ S R                 +V  ++ +GLL+L                 L 
Sbjct: 196 FIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILV 255

Query: 254 SPLVIPIYTFFKSWHSNQDRSDMEARIEEPLLKEEAVAAPVKEEAEEIAEEEQRAPVIGE 313
             +V+P+   F S   +   +    + EE     +   A    E  +   E++RAP IGE
Sbjct: 256 IFMVVPVLVPFSSVFIS-GNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKKRAPCIGE 315

Query: 314 EHTIFEAVKTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFG 373
           + T+ +A+   DFW++F+S + GVG+G+ +++N+GQI  +LGY++  +FVSL SI  F G
Sbjct: 316 DFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLISISNFLG 375

Query: 374 RILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYILMAMAMPGSLYIGSVIVGICYGVRL 433
           R+  G  SE  ++K   PR L  +  Q +M++G I  A+  PG +Y+ ++++G+ YG   
Sbjct: 376 RVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAHW 435

Query: 434 SVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATDGAAGTHASEGI 459
           ++   + S++FGLK +G +YN  I  LPIGSF+FSG++A  +YD  A   A  T  +E +
Sbjct: 436 AIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPTTETESL 493