HG10000463 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000463
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionisoflavone reductase-like protein
LocationChr09: 5474177 .. 5476206 (+)
RNA-Seq ExpressionHG10000463
SyntenyHG10000463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAGAATCTCAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGAAAATTCATAGTGCAAGCCAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGTATCGAATCATCAATCATTTCAAAAGCTTAGGGGTCAATTTTCTCTTCGTAAGTAGATTGTCCTCTATTTCTCTGGATTTTGTTGTTTCGTTGCTCAGAATTCTTGTGGAATCGATTGCTGAATAATGTTCCTGATTCTTTTTCTATTTTGTTCGCGAATGAGTAAACTAGGTTTGATTTGTTTCAGTCGAAGTGATTACTTATAATGTAGCATTTACTGTTGGTTCTTTTGAGTTATTCACATCATTAATTCTCAAATTCTGTGGAATCATAATGAATTTGATTGGAAATAGTATTTAATTTCGCTAAAACGCTAAAAATTGGGGCAGGGCGATCTCTTTGACAATGAGAGTCTGGTTAAGGCGATTAAGCAGGTTGATGTAGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTAAAGGTAACTTTAAACATTTTGCCTACAACTATGTCATTGATTGTGGTTATTTTGCTGCAAAATCCATTCTCCTAGTAGAGTTTGTATGTTTTGGGAATTTTACGAATTAGAATTTGTGTGGTACAGAGATTCTTTCCTTCCGAGTTCGGCAACGACGCGGATCATATCGATGCAGTGGAGCCAGCAAAATCAATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTGTGGAGGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAACCGTCCCACCTAGAGACAAAGTCGTAATATTAGGAGATGGAACTCCAAAAGGTACAATATCTTACTTGTAAGATGATATAATATTAAATTTACTTTTGCCCATTAGCTTAAGTTTTTGAGTCAAAAGGTGATTTAAAATGGTATTAAAGCAGGAGGTTTTGTGTTTAAACTACTGCAATGTTATTTCCTCCTCAATTAATATTGATTTTCACTCGTGAGGTCTTTTGTTGTGGGGTGTGTTAGATGACATAATATTAAATTTATCTTTACCCATTAGTTTAAGCTTTTGGGTCAATCGTTGATTTAACATTACTTTCATTCTCTTGAGTTCTGATGGAACTTTTTCTTTTGTCGGAGGGATTGTTGTACAATACTGTATCCCATTTTCTGGAGTAGAATGGTTTTCAAAGTGGCAACCCCCCCCCCCTGGAAATCAAATAGATAATGATGCGTTTCATTGGTTTTTTATTCAGCTAAACCTTATGCATAACATAACATGGACACATGTTGTATATTATTGTCTGCAATATGGATTTTGTTTCCATAGCCTCCCACACTATTCATTCCTTGATTGAAGGGAGGTATAGATTTTGTTTCAACAGCCATTCACCCAATTCATTCTGTGTCTGTTTTGTTTCATGAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACAATCCGAGCAATAGATGATCCAAGGACCCTCAACAAGATCATGTACCTTAGACCCCCAGCCAACATCTACTCAACCAATGATCTCATCTCTCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGGATCTACGTCCCAGAGGAGGAAGTGTTGAAGAAAATCAAAGGTGGGTTTATTTAGCAAAGCAACAAATTTTCAATTCTTATTCTACCATATGCTTCTAAAACACAGACTGATTAACACATTTTGTTTTTGCTAATCATCTTTTTCAGAGACTTCATATCCATTTAATATAGAATTAGCACTCTGTCACACTGCTCAGGTGAAGGGATCTCAAACTAATTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCATTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTTGACCAGTTTTTCTAA

mRNA sequence

ATGGCTCAGAATCTCAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGAAAATTCATAGTGCAAGCCAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGTATCGAATCATCAATCATTTCAAAAGCTTAGGGGTCAATTTTCTCTTCGGCGATCTCTTTGACAATGAGAGTCTGGTTAAGGCGATTAAGCAGGTTGATGTAGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTAAAGAGATTCTTTCCTTCCGAGTTCGGCAACGACGCGGATCATATCGATGCAGTGGAGCCAGCAAAATCAATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTGTGGAGGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAACCGTCCCACCTAGAGACAAAGTCGTAATATTAGGAGATGGAACTCCAAAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACAATCCGAGCAATAGATGATCCAAGGACCCTCAACAAGATCATGTACCTTAGACCCCCAGCCAACATCTACTCAACCAATGATCTCATCTCTCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGGATCTACGTCCCAGAGGAGGAAGTGTTGAAGAAAATCAAAGAGACTTCATATCCATTTAATATAGAATTAGCACTCTGTCACACTGCTCAGGTGAAGGGATCTCAAACTAATTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCATTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTTGACCAGTTTTTCTAA

Coding sequence (CDS)

ATGGCTCAGAATCTCAAGGTTCTTATCATCGGAGGAACTGGCTACATCGGAAAATTCATAGTGCAAGCCAGCGCCAAAGCCGGTCATCCTACCTATGCATTGATCCGCAGGTCTTCGCTCGAAAGCCCCGCCAAGTATCGAATCATCAATCATTTCAAAAGCTTAGGGGTCAATTTTCTCTTCGGCGATCTCTTTGACAATGAGAGTCTGGTTAAGGCGATTAAGCAGGTTGATGTAGTGATATCCACCCTCGGTGGTCATATGGTTCCCCATCAACATAAGATCCTATCTGCTATCAAACAAGCTGGCAATGTAAAGAGATTCTTTCCTTCCGAGTTCGGCAACGACGCGGATCATATCGATGCAGTGGAGCCAGCAAAATCAATGTATGCTGCAAAGGCTGAATTTCGTAGGGCTGTGGAGGCTGAGGGAATCCCACATACATTTGTGGTATGCAATTTCTTCGATGGTTACTTTCTTAGCAACTTGTCTCAGCCAGATGCAACCGTCCCACCTAGAGACAAAGTCGTAATATTAGGAGATGGAACTCCAAAAGTGATTTACAACAAGGAAGAAGACGTTGGGACGTACACAATCCGAGCAATAGATGATCCAAGGACCCTCAACAAGATCATGTACCTTAGACCCCCAGCCAACATCTACTCAACCAATGATCTCATCTCTCTGTGGGAGAGAAAAATTGGCAAGAGTCTTAAAAGGATCTACGTCCCAGAGGAGGAAGTGTTGAAGAAAATCAAAGAGACTTCATATCCATTTAATATAGAATTAGCACTCTGTCACACTGCTCAGGTGAAGGGATCTCAAACTAATTTTGATATTGAGCCATCTTTTGGAGTGGAAGCTTCAGCATTGTACCCAGATGTTCGATATACAACTGTGGAAGAATACCTTGACCAGTTTTTCTAA

Protein sequence

MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLFGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKRIYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVEEYLDQFF
Homology
BLAST of HG10000463 vs. NCBI nr
Match: XP_038900572.1 (phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida])

HSP 1 Score: 607.4 bits (1565), Expect = 6.6e-170
Identity = 298/307 (97.07%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIR+SSLESP+K R++NHFKSLGVNFL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLESPSKLRVLNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG
Sbjct: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAI+DPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAINDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLK I+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKNIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 307

BLAST of HG10000463 vs. NCBI nr
Match: XP_008460945.1 (PREDICTED: isoflavone reductase-like protein [Cucumis melo])

HSP 1 Score: 601.3 bits (1549), Expect = 4.7e-168
Identity = 298/307 (97.07%), Postives = 303/307 (98.70%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 305

BLAST of HG10000463 vs. NCBI nr
Match: KAA0045560.1 (isoflavone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 599.4 bits (1544), Expect = 1.8e-167
Identity = 297/306 (97.06%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQ 307
           EEYLDQ
Sbjct: 301 EEYLDQ 304

BLAST of HG10000463 vs. NCBI nr
Match: TYK28833.1 (isoflavone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 599.4 bits (1544), Expect = 1.8e-167
Identity = 297/306 (97.06%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQ 307
           EEYLDQ
Sbjct: 301 EEYLDQ 304

BLAST of HG10000463 vs. NCBI nr
Match: XP_004148838.1 (phenylcoumaran benzylic ether reductase POP1 [Cucumis sativus] >KGN61803.1 hypothetical protein Csa_006751 [Cucumis sativus])

HSP 1 Score: 597.8 bits (1540), Expect = 5.2e-167
Identity = 296/307 (96.42%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMYAAKAEFRRA+EAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKG QTNF IEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 305

BLAST of HG10000463 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 8.8e-125
Identity = 210/302 (69.54%), Postives = 257/302 (85.10%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLFGDLF 65
           K+L IGGTGYIGKFIV+ASAKAGHPT+ L+R S+L +PAK  +I +FK+LGVNFL GDLF
Sbjct: 4   KILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDLF 63

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q +I++AIK+AGNVKRFFPSEFGND D ++AVEP
Sbjct: 64  DHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVEP 123

Query: 126 AKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILGDGTPK 185
           AKS +A KA  RRA+EAEGIP+T+V  NFF GYFL + +QP AT PPRDKVVILGDG PK
Sbjct: 124 AKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNPK 183

Query: 186 VIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKRIYVPE 245
            ++NKE+D+ TYTI+A+DDPRTLNKI+Y++PPAN  S NDL+SLWE+KIGK+L+RIYVPE
Sbjct: 184 AVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVPE 243

Query: 246 EEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVEEYLD 305
           E++LK I+E S P N+ L++ H+  VKG  TNF+IEPSFGVEAS LYPDV+YTTV+EYL 
Sbjct: 244 EQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYLK 303

Query: 306 QF 308
           QF
Sbjct: 304 QF 305

BLAST of HG10000463 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 5.7e-124
Identity = 210/307 (68.40%), Postives = 258/307 (84.04%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MA   ++L IGGTGYIGKFIV+ASAKAG+PTY L+R +SL  PAK ++I +FK+LGVNF+
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            GDL+D+ESLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPSEFGND D  
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
            AVEPAKS +  KA+ RRAVEAEGIP+T+V  NFF GYFL  L+QP A+  PRDKVVILG
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DG PK I+NKE+D+GTYTIRA+DDPRTLNK++Y+RPPAN  S N+L+SLWE+KIGK+L+R
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEE++LK I+E + P N+ L++ H   VKG  TNF+IEPSFGVEA+ALYPDV+YTTV
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 301 EEYLDQF 308
           +EYL+QF
Sbjct: 301 DEYLNQF 307

BLAST of HG10000463 vs. ExPASy Swiss-Prot
Match: Q9T030 (Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCBER1 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 9.1e-122
Identity = 204/307 (66.45%), Postives = 252/307 (82.08%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           M    K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L SP++   I +FK+LGV FL
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            GDL D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFPSEFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
             VEPAKS YA KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP AT  PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DG PK ++NKEED+GTYTI A+DDPRTLNKI+Y+RPP N YS NDL+SLWE KIGK+L+R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEE++LK+I E+S P N+ L+LCH   VKG  T+F+IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQF 308
           +E L+Q+
Sbjct: 301 DEILNQY 307

BLAST of HG10000463 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-121
Identity = 206/308 (66.88%), Postives = 256/308 (83.12%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           M    K+LIIGGTGYIGKFIV+AS K GHPT+AL+R +++  P K +++  F++LGV+ L
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           +GDL+D++SLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPSEFGND DH+
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 121 DAVEPAKSM-YAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVIL 180
           +AVEPAKS+ +A KA  RRAVEAEGIP+T+V  N F+GYFL  L QP AT PPRDKV+I 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 181 GDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLK 240
           GDG PK I+NKEED+GTYTI+A+DDPRTLNKI+YLRP  NIYS N+L++LWE+KIGK+L+
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 241 RIYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTT 300
           +IYVPEE++LK I+E   P NI L + H+  VKG  TNF+IEPSFGVEAS LYP+V+YTT
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 301 VEEYLDQF 308
           VEEYLDQF
Sbjct: 301 VEEYLDQF 308

BLAST of HG10000463 vs. ExPASy Swiss-Prot
Match: E1U332 (Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.6e-118
Identity = 202/307 (65.80%), Postives = 251/307 (81.76%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MA   K+LIIGGTGYIGKFIV+ASAK+ HPT+AL R S++  P K +II  FK+ GV  L
Sbjct: 1   MADKTKILIIGGTGYIGKFIVEASAKSEHPTFALARESTISDPVKGKIIQGFKNSGVTIL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            GDL+D+ESLVKAIKQVDVVIST+G   +  Q KI++AIK+AGNVKRFFPS+FG D D  
Sbjct: 61  TGDLYDHESLVKAIKQVDVVISTVGQLQLADQVKIIAAIKEAGNVKRFFPSDFGTDVDRC 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
            AVEPAKS +  K++ RRA+EAEGIP+TFV  N+F GY L  L QP+ T PPRDKV+ILG
Sbjct: 121 HAVEPAKSSFEIKSQIRRAIEAEGIPYTFVSANYFAGYSLPTLVQPEVTAPPRDKVIILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DG  K ++N+E D+GTYTI+A+DD RTLNKI+Y++PP NIYS N+L++LWE+KIGK+L++
Sbjct: 181 DGNAKAVFNEENDIGTYTIKAVDDARTLNKILYIKPPKNIYSFNELVALWEKKIGKTLEK 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEE+VLK+I+E+ +P NI +A+ H+A VKG  TNF IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEQVLKQIQESPFPINIVMAINHSAFVKGDLTNFKIEPSFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 307

BLAST of HG10000463 vs. ExPASy TrEMBL
Match: A0A1S3CE33 (isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.3e-168
Identity = 298/307 (97.07%), Postives = 303/307 (98.70%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 305

BLAST of HG10000463 vs. ExPASy TrEMBL
Match: A0A5A7TR10 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00570 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 8.7e-168
Identity = 297/306 (97.06%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQ 307
           EEYLDQ
Sbjct: 301 EEYLDQ 304

BLAST of HG10000463 vs. ExPASy TrEMBL
Match: A0A5D3DYL6 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00390 PE=4 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 8.7e-168
Identity = 297/306 (97.06%), Postives = 302/306 (98.69%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY+AKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYSAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQ 307
           EEYLDQ
Sbjct: 301 EEYLDQ 304

BLAST of HG10000463 vs. ExPASy TrEMBL
Match: A0A0A0LJ01 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248130 PE=4 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.5e-167
Identity = 296/307 (96.42%), Postives = 301/307 (98.05%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQ  KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAK RI+NHFKSLGVNFL
Sbjct: 1   MAQ--KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKNRILNHFKSLGVNFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMYAAKAEFRRA+EAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYAAKAEFRRAIEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDL+SLWERKIGKSLKR
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLVSLWERKIGKSLKR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEEEVLKKI+ETSYP NIELALCHTAQVKG QTNF IEPSFGVEASALYPDVRYTTV
Sbjct: 241 IYVPEEEVLKKIRETSYPLNIELALCHTAQVKGCQTNFSIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 305

BLAST of HG10000463 vs. ExPASy TrEMBL
Match: A0A6J1FXY6 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.5e-164
Identity = 287/307 (93.49%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIR+SSL S AK +IINHFKSLGV FL
Sbjct: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRKSSLGSSAKSQIINHFKSLGVKFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
           FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFP+EFGNDADHI
Sbjct: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPTEFGNDADHI 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
           DAVEPAKSMY AKAEFRR VEAEGIPHTFVVCNFFDGYFLSNLSQPDA+VPPRDKVVILG
Sbjct: 121 DAVEPAKSMYTAKAEFRRVVEAEGIPHTFVVCNFFDGYFLSNLSQPDASVPPRDKVVILG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMY+RPPANIYSTNDL+SLWERKIGK+L+R
Sbjct: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYIRPPANIYSTNDLVSLWERKIGKTLRR 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           +YVPEEEVLK I+E SYP NIELALCHTAQV+GSQTNFDIEPSFGVEASALYPDVRYTTV
Sbjct: 241 VYVPEEEVLKNIQEASYPLNIELALCHTAQVRGSQTNFDIEPSFGVEASALYPDVRYTTV 300

Query: 301 EEYLDQF 308
           EEYLDQF
Sbjct: 301 EEYLDQF 307

BLAST of HG10000463 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 438.0 bits (1125), Expect = 6.5e-123
Identity = 204/307 (66.45%), Postives = 252/307 (82.08%), Query Frame = 0

Query: 1   MAQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFL 60
           M    K+L IGGTGYIGK+IV+ASA++GHPT  L+R S+L SP++   I +FK+LGV FL
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  FGDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHI 120
            GDL D+ SLV +IKQ DVVIST+G  ++ HQ+KI+SAIK+AGNVKRFFPSEFGND D +
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 DAVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATVPPRDKVVILG 180
             VEPAKS YA KA+ RR +EAEGIP+T+V CNFF GYFL  L+QP AT  PRDKV++LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKR 240
           DG PK ++NKEED+GTYTI A+DDPRTLNKI+Y+RPP N YS NDL+SLWE KIGK+L+R
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 IYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTV 300
           IYVPEE++LK+I E+S P N+ L+LCH   VKG  T+F+IEPSFGVEAS LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 EEYLDQF 308
           +E L+Q+
Sbjct: 301 DEILNQY 307

BLAST of HG10000463 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 378.6 bits (971), Expect = 4.7e-105
Identity = 183/304 (60.20%), Postives = 230/304 (75.66%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLFGDLF 65
           K+L+IGGTGYIGKF+V+ASAKAGH T+AL+R ++L  P K + +  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVEP 126

Query: 126 AKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDATVPPRDKVVILGDGT 185
           AKS +A K + RR +EAEGIP+T+ V   F GY+L  L Q  P  T PPRDKV ILGDG 
Sbjct: 127 AKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDGN 186

Query: 186 PKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKRIYV 245
            K + NKEED+  YTI+A+DDPRTLNKI+Y++P  N  S N++++LWE+KIGKSL++ ++
Sbjct: 187 AKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTHL 246

Query: 246 PEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVEEY 305
           PEE++LK I+E+  P N+ L++ H   V G  TN  IEPSFGVEAS LYPDV+YT+V+EY
Sbjct: 247 PEEQLLKSIQESPIPINVVLSINHAVFVNG-DTNISIEPSFGVEASELYPDVKYTSVDEY 306

Query: 306 LDQF 308
           L  F
Sbjct: 307 LSYF 309

BLAST of HG10000463 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 1.4e-104
Identity = 178/308 (57.79%), Postives = 230/308 (74.68%), Query Frame = 0

Query: 2   AQNLKVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLF 61
           ++  K+L+IGGTG+IGK I++AS KAGH T AL+R +SL  P K + + +FK  GV  L 
Sbjct: 3   SEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLH 62

Query: 62  GDLFDNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHID 121
           GDL D+ESLVKAIKQ DVVIST+G   +  Q KI+SAIK+AGNVKRF PSEFG D D   
Sbjct: 63  GDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSS 122

Query: 122 AVEPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ--PDATVPPRDKVVIL 181
           AVEPAKS +  K + RR +EAEGIP+T++V N+F GY+L  L Q  P  T PPRDKV I 
Sbjct: 123 AVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIF 182

Query: 182 GDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLK 241
           GDG  K + NKEED+  YTI+A+DDPRTLNK +Y+ PP N  S N++++LWE+KIGKS++
Sbjct: 183 GDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVE 242

Query: 242 RIYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTT 301
           +IY+ EE++ K I+E+  PFN+ L++ H   VKG QTNF IEPSFG EAS LYPD++YT+
Sbjct: 243 KIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTS 302

Query: 302 VEEYLDQF 308
           ++EYL  F
Sbjct: 303 IDEYLSYF 310

BLAST of HG10000463 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.7e-99
Identity = 179/318 (56.29%), Postives = 230/318 (72.33%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLFGDLF 65
           K+L+IGGTGY+G+FIV+ SAKAG+PT+AL+R +SL  P K + I  FK LGV  L GDL 
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 66  DNESLVKAIKQVDVVISTLGGHMVPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAVEP 125
           D+ESLVKAIKQVDVVIST+G   +  Q KI+SAIK+AGNVKRF P+EFG D +   AVEP
Sbjct: 67  DHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAVEP 126

Query: 126 AKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQ----------------PDAT 185
           AKS++A K + RRA+EAEGIP+T+VV N   G++L  L Q                 D  
Sbjct: 127 AKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGDKN 186

Query: 186 VPPRDKVVILGDGTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISL 245
           VPPRDKV ILGDG  KV+ NKEEDV  Y I+A+DD RTLNK +Y+ PP NI S N++++L
Sbjct: 187 VPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVTL 246

Query: 246 WERKIGKSLKRIYVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEAS 305
           WE+KIGKSL++ ++ EE++LK I+    P ++  ++ H   VKG QT+F IEP FG EAS
Sbjct: 247 WEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEEAS 306

Query: 306 ALYPDVRYTTVEEYLDQF 308
            LYPDV+YT+++EYL QF
Sbjct: 307 VLYPDVKYTSIDEYLSQF 321

BLAST of HG10000463 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 331.3 bits (848), Expect = 8.5e-91
Identity = 165/306 (53.92%), Postives = 216/306 (70.59%), Query Frame = 0

Query: 6   KVLIIGGTGYIGKFIVQASAKAGHPTYALIRRSSLESPAKYRIINHFKSLGVNFLFGDLF 65
           K+L+IG TG IGK +V+ SAK+GH T+AL+R +SL  P K +++  FK LGV  L+G L 
Sbjct: 4   KILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSLS 63

Query: 66  DNESLVKAIKQVDVVISTLGGHM--VPHQHKILSAIKQAGNVKRFFPSEFGNDADHIDAV 125
           D ESLVKAIKQVDVVIS +G     + +Q  I+ AIK++GNVKRF PSEFGND D   A+
Sbjct: 64  DKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVAI 123

Query: 126 EPAKSMYAAKAEFRRAVEAEGIPHTFVVCNFFDGYFLSNLSQPDATV--PPRDKVVILGD 185
           EP  S +  KA+ RRA+EA  IP+T+VV   F G F+  L Q    +  PPRDKV I   
Sbjct: 124 EPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYDT 183

Query: 186 GTPKVIYNKEEDVGTYTIRAIDDPRTLNKIMYLRPPANIYSTNDLISLWERKIGKSLKRI 245
           G  K I N EED+  YT++A+DDPRTLNKI+Y+ PP  I S ND++ LWE KIGK+L++ 
Sbjct: 184 GNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEKT 243

Query: 246 YVPEEEVLKKIKETSYPFNIELALCHTAQVKGSQTNFDIEPSFGVEASALYPDVRYTTVE 305
           YV EEE+LK I+E+  P +  + L HT  VK   T+F I+PSFGVEAS LYP+V+YT+V+
Sbjct: 244 YVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSVD 303

Query: 306 EYLDQF 308
           E+L++F
Sbjct: 304 EFLNRF 309

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900572.16.6e-17097.07phenylcoumaran benzylic ether reductase POP1-like [Benincasa hispida][more]
XP_008460945.14.7e-16897.07PREDICTED: isoflavone reductase-like protein [Cucumis melo][more]
KAA0045560.11.8e-16797.06isoflavone reductase-like protein [Cucumis melo var. makuwa][more]
TYK28833.11.8e-16797.06isoflavone reductase-like protein [Cucumis melo var. makuwa][more]
XP_004148838.15.2e-16796.42phenylcoumaran benzylic ether reductase POP1 [Cucumis sativus] >KGN61803.1 hypot... [more]
Match NameE-valueIdentityDescription
B9HRL78.8e-12569.54Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
O813555.7e-12468.40Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
Q9T0309.1e-12266.45Phenylcoumaran benzylic ether reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PCB... [more]
B2WSN01.2e-12166.88Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
E1U3323.6e-11865.80Isoflavone reductase-like protein OS=Olea europaea OX=4146 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CE332.3e-16897.07isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103499680 PE=4 S... [more]
A0A5A7TR108.7e-16897.06Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3DYL68.7e-16897.06Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0LJ012.5e-16796.42NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248130 PE=4 S... [more]
A0A6J1FXY61.5e-16493.49isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448291 ... [more]
Match NameE-valueIdentityDescription
AT4G39230.16.5e-12366.45NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.14.7e-10560.20NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.11.4e-10457.79NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.11.7e-9956.29NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.18.5e-9153.92NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 7..271
e-value: 4.4E-75
score: 252.2
NoneNo IPR availableGENE3D3.40.50.720coord: 6..294
e-value: 3.1E-133
score: 445.4
NoneNo IPR availableGENE3D3.90.25.10coord: 154..304
e-value: 3.1E-133
score: 445.4
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 1..307
NoneNo IPR availablePANTHERPTHR43349:SF35PHENYLCOUMARAN BENZYLIC ETHER REDUCTASE 1coord: 1..307
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 6..303
e-value: 8.23286E-85
score: 254.535
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000463.1HG10000463.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor