HG10000440 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000440 vs. NCBI nr
Match: XP_038902921.1 (phosphatidylinositol 4-kinase alpha 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 3904.0 bits (10123), Expect = 0.0e+00
Identity = 1971/2037 (96.76%), Postives = 2002/2037 (98.28%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNA+LA+ARFLSKC
Sbjct: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAILAVARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA
Sbjct: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            +VAVYTGEVVASAISSGGGD GISRSFLMVFSKHFLPILPSDANKLVDMVIERYLM GS 
Sbjct: 121  DVAVYTGEVVASAISSGGGDLGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRN-GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240
            APRE MQGNSDSSA  SIDGSSPRN GSHDSGSRVGDDNISSWKS+ATSYGSGILWKSS+
Sbjct: 181  APRELMQGNSDSSASHSIDGSSPRNEGSHDSGSRVGDDNISSWKSTATSYGSGILWKSSS 240

Query: 241  DQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300
            DQLGPMFG NDGGGGGE+LLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD
Sbjct: 241  DQLGPMFGLNDGGGGGEILLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300

Query: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360
            QVR+IAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVY+AAAKLKLKSLGSLDSEG
Sbjct: 301  QVRAIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYKAAAKLKLKSLGSLDSEG 360

Query: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420
            KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI
Sbjct: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420

Query: 421  RLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGR 480
            RLKPLVLTVCAQTDPSDS H  MFETVSKT+CEI+ESCWTKERAPVDTFIMGLTASIRGR
Sbjct: 421  RLKPLVLTVCAQTDPSDSSHGGMFETVSKTMCEIIESCWTKERAPVDTFIMGLTASIRGR 480

Query: 481  NDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540
            NDYEEQVN+EKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR
Sbjct: 481  NDYEEQVNREKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540

Query: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600
            LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP
Sbjct: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600

Query: 601  AGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660
             GFLLIASGL SSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF
Sbjct: 601  TGFLLIASGLTSSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660

Query: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720
            SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG
Sbjct: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720

Query: 721  PYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780
            PYMWDK+WSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA
Sbjct: 721  PYMWDKRWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780

Query: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVF 840
            LATALGGRVDISAM+TISGVKATYLLAVSFLEITRFSSNGGILNGSI+VDASRSAFSCVF
Sbjct: 781  LATALGGRVDISAMNTISGVKATYLLAVSFLEITRFSSNGGILNGSINVDASRSAFSCVF 840

Query: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSM 900
            EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAA+RNSILNAHTCFLIKSM
Sbjct: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAVRNSILNAHTCFLIKSM 900

Query: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSL 960
            CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSL
Sbjct: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSL 960

Query: 961  YQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020
            YQKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW
Sbjct: 961  YQKVVREWIIRSLSHAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020

Query: 1021 TGI-QTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN 1080
            +GI QTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN
Sbjct: 1021 SGILQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN 1080

Query: 1081 TFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAE 1140
            TFGGYQSAGA PTGSGIGIGLQRLITGAFPQ+PQGEDESFNAMLLLKFVQSLQKFVNNAE
Sbjct: 1081 TFGGYQSAGAAPTGSGIGIGLQRLITGAFPQDPQGEDESFNAMLLLKFVQSLQKFVNNAE 1140

Query: 1141 TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV
Sbjct: 1141 TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGCV+LAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM
Sbjct: 1201 FVWTWLVSAAPQLGCVILAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQSQVNLQNFK+GLQLLEDRVYRASLGWFAFEPEWH+INSTNFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKSGLQLLEDRVYRASLGWFAFEPEWHEINSTNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQ+ENYTVGREKR+QL
Sbjct: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQMENYTVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NVTLRAEVSQQVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL
Sbjct: 1501 NVTLRAEVSQQVQSHILDIRYIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSD+F+
Sbjct: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDLFA 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPVVRQQIIDGFTGK 1680
            HILIWH              LQGETCEPE GKEATSTK+NSFQALLPVVRQ IIDGF+ K
Sbjct: 1621 HILIWH--------------LQGETCEPEMGKEATSTKSNSFQALLPVVRQHIIDGFSEK 1680

Query: 1681 ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVKG 1740
            ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGI+RELEKIEMNGDDLYLPTAPSKLVKG
Sbjct: 1681 ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMNGDDLYLPTAPSKLVKG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR 1800
            IQVDSGIPLQSAAKVPIMITFNV DRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMR 1920
            PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGI+TTVALMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGIITTVALMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2023

BLAST of HG10000440 vs. NCBI nr
Match: XP_008457937.1 (PREDICTED: phosphatidylinositol 4-kinase alpha 1 [Cucumis melo])

HSP 1 Score: 3880.5 bits (10062), Expect = 0.0e+00
Identity = 1961/2036 (96.32%), Postives = 1988/2036 (97.64%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEALLEL DLIAQNPSAFFEK++WICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSK 
Sbjct: 1    MEALLELSDLIAQNPSAFFEKLSWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKS 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSIT FYT+FLGYVSKATEITPDFSA
Sbjct: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITSFYTDFLGYVSKATEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGE+VASAISSGGGDSGISR FLMVFSK+FLP+LPSDANKLVDMVIERYLM GS 
Sbjct: 121  EVAVYTGEIVASAISSGGGDSGISRVFLMVFSKNFLPVLPSDANKLVDMVIERYLMTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRN-GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240
            APREQMQGNSDSSAP S+DGS P+N GSHDSGSRVGDDNISSWKSSATSYGSGILWKSS 
Sbjct: 181  APREQMQGNSDSSAPHSVDGSVPQNGGSHDSGSRVGDDNISSWKSSATSYGSGILWKSST 240

Query: 241  DQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300
            DQLGPMFGFNDGGGGGE+LLKQQVS FEEESLESLEKQAIAFE+LAHILDKARIDSWLWD
Sbjct: 241  DQLGPMFGFNDGGGGGEILLKQQVSGFEEESLESLEKQAIAFEMLAHILDKARIDSWLWD 300

Query: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360
            QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG
Sbjct: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360

Query: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420
            KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI
Sbjct: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420

Query: 421  RLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGR 480
            RLKPLVLTVCAQTDPSDS  N+MFETVSKT+C I+ESCWTKERAPVDTFIMGLTASIRGR
Sbjct: 421  RLKPLVLTVCAQTDPSDSSPNSMFETVSKTMCGIIESCWTKERAPVDTFIMGLTASIRGR 480

Query: 481  NDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540
            NDYEEQ NKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR
Sbjct: 481  NDYEEQENKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540

Query: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600
            LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSA APEATTERLETLP
Sbjct: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAAAPEATTERLETLP 600

Query: 601  AGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660
            AGFLLIA GLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF
Sbjct: 601  AGFLLIARGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660

Query: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720
            SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVS AGSVGTTAIQAVSG
Sbjct: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSIAGSVGTTAIQAVSG 720

Query: 721  PYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780
            PYMWDKQWSSAVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA
Sbjct: 721  PYMWDKQWSSAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780

Query: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVF 840
            LATALGGRVDISAM TISGVKATYLLAVSFLEITRFSSNGGILNGS SVDASRSAFSCVF
Sbjct: 781  LATALGGRVDISAMGTISGVKATYLLAVSFLEITRFSSNGGILNGSTSVDASRSAFSCVF 840

Query: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSM 900
            EYLKTPNLVPAVLQCLTALVHRAYETAVSWLE+RISEIGNEA +RNSILNAHTCFLIKS+
Sbjct: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEKRISEIGNEATVRNSILNAHTCFLIKSL 900

Query: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSL 960
            CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSL
Sbjct: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSL 960

Query: 961  YQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020
            YQKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQ TPDVASLLSEIRIGTGKNDCW
Sbjct: 961  YQKVVREWIIRSLSHAPCTSQGLLQEMLCKANTWQRAQQTPDVASLLSEIRIGTGKNDCW 1020

Query: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT 1080
            TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT
Sbjct: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT 1080

Query: 1081 FGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140
            FGGYQSAG  PTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 FGGYQSAGPAPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200
            GGEIDKSQFHETCSQATAFLLANLGTDSK NIEGLSQLLRLLCWCPAYISTSNAMETGVF
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKFNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200

Query: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260
            VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP
Sbjct: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260

Query: 1261 PEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATGS 1320
            PEIDPV QIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSM+LPWKFSHHPAATGS
Sbjct: 1261 PEIDPVGQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMQLPWKFSHHPAATGS 1320

Query: 1321 FFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380
            FFTLMFLGLKFCSCQSQVNLQNFK GLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE
Sbjct: 1321 FFTLMFLGLKFCSCQSQVNLQNFKAGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380

Query: 1381 AQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQLL 1440
            AQSVALFVQYLSNYLKDGSQ DVKGQVRENGN  D NSCHPVWGQ+ENYTVGREKR+QLL
Sbjct: 1381 AQSVALFVQYLSNYLKDGSQTDVKGQVRENGNLADQNSCHPVWGQMENYTVGREKRRQLL 1440

Query: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN 1500
            LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN
Sbjct: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN 1500

Query: 1501 VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560
            VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA
Sbjct: 1501 VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560

Query: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFSH 1620
            PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIF+H
Sbjct: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFAH 1620

Query: 1621 ILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPVVRQQIIDGFTGKA 1680
            ILIWH              LQGETCEPETGKEATSTK+NSFQALLPVVRQ IIDGF+GKA
Sbjct: 1621 ILIWH--------------LQGETCEPETGKEATSTKSNSFQALLPVVRQHIIDGFSGKA 1680

Query: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVKGI 1740
            LDVFQREFDFFDKVTSISGVLFPLPKDERRAGI+RELEKIEMNGDDLYLPTAPSKLVKGI
Sbjct: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMNGDDLYLPTAPSKLVKGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRD 1800
            QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVISLLRD 1800

Query: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRF 1920
            SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGI+TTVALMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGIITTVALMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2022

BLAST of HG10000440 vs. NCBI nr
Match: XP_011649229.1 (phosphatidylinositol 4-kinase alpha 1 [Cucumis sativus])

HSP 1 Score: 3869.3 bits (10033), Expect = 0.0e+00
Identity = 1953/2036 (95.92%), Postives = 1986/2036 (97.54%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEALLEL DLIAQNPSAF+EK++WICSRCPP DALLSDSPRVSRSQLNAVLAIARFLSK 
Sbjct: 1    MEALLELSDLIAQNPSAFYEKLSWICSRCPPSDALLSDSPRVSRSQLNAVLAIARFLSKS 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            ADSVEPRPKAVVLEFIRSIPSSFN SFWPPTYGNDSIT FYT+FLGYVSKATEITPDFSA
Sbjct: 61   ADSVEPRPKAVVLEFIRSIPSSFNQSFWPPTYGNDSITSFYTDFLGYVSKATEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISSGGGDSGISR FLMVFSK+FLPI+PSDANKLVDMVIERYLM GS 
Sbjct: 121  EVAVYTGEVVASAISSGGGDSGISRVFLMVFSKNFLPIMPSDANKLVDMVIERYLMTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRN-GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240
            APRE +QGNSDSSAP S DGS P+N GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA
Sbjct: 181  APREHLQGNSDSSAPHSTDGSIPQNGGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240

Query: 241  DQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300
            DQLGPMFGFNDGGGGGE+LLKQQVS FEEESLESLEKQAIAFELLAHILDKARIDSWLWD
Sbjct: 241  DQLGPMFGFNDGGGGGEILLKQQVSGFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300

Query: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360
            QVRSIAKKQLQSLLIFVKIRKRDWNEQGS LKARINAKLLVYQAAAKLKLKSLGSLDSEG
Sbjct: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSFLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360

Query: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420
            KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI
Sbjct: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420

Query: 421  RLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGR 480
            RLKPLVLTVCAQTDP DS  N+MFETVSKT+C I+E+CWTKERAPVDTFIMGLTASIRGR
Sbjct: 421  RLKPLVLTVCAQTDPYDSSPNSMFETVSKTMCGIIEACWTKERAPVDTFIMGLTASIRGR 480

Query: 481  NDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540
            NDYEEQ NKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR
Sbjct: 481  NDYEEQENKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540

Query: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600
            LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP
Sbjct: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600

Query: 601  AGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660
            AGFLL+A GLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF
Sbjct: 601  AGFLLVAKGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660

Query: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720
            SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVS AGSVGTTAIQAVSG
Sbjct: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSIAGSVGTTAIQAVSG 720

Query: 721  PYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780
            PYMWDKQWSSAVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA
Sbjct: 721  PYMWDKQWSSAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780

Query: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVF 840
            LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGS SVDASRSAFSCVF
Sbjct: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSTSVDASRSAFSCVF 840

Query: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSM 900
            EYLKTPNLVPAVLQCLTALVH+AYETAVSWLEQRISEIGNEA +RNSILNAHTCFLIKS+
Sbjct: 841  EYLKTPNLVPAVLQCLTALVHKAYETAVSWLEQRISEIGNEATVRNSILNAHTCFLIKSL 900

Query: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSL 960
            CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVT+RSL
Sbjct: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTIRSL 960

Query: 961  YQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020
            YQKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW
Sbjct: 961  YQKVVREWIIRSLSHAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020

Query: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT 1080
            TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGM+RLYNT
Sbjct: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMKRLYNT 1080

Query: 1081 FGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140
            FGGYQSAG  PTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 FGGYQSAGPAPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200
            GGEIDKSQFHETCSQATAFLLANLGTDSK NIEGLSQLLRLLCWCPAYISTSNAMETGVF
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKFNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200

Query: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260
            VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP
Sbjct: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260

Query: 1261 PEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATGS 1320
            PEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSM+LPWKFSHHPAATGS
Sbjct: 1261 PEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMQLPWKFSHHPAATGS 1320

Query: 1321 FFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380
            FFTLMFLGLKFCSCQSQVNLQNFK GLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE
Sbjct: 1321 FFTLMFLGLKFCSCQSQVNLQNFKAGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380

Query: 1381 AQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQLL 1440
            AQSVALFVQYLSNYLKDGSQ DVKGQVRENG+ VD N CHP+WGQ+ENYTVGRE+R+QLL
Sbjct: 1381 AQSVALFVQYLSNYLKDGSQTDVKGQVRENGSVVDQNFCHPIWGQMENYTVGRERRRQLL 1440

Query: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN 1500
            LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVE+ARTAYSVDPRIAFSLVSRFPAN
Sbjct: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEHARTAYSVDPRIAFSLVSRFPAN 1500

Query: 1501 VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560
            +TLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA
Sbjct: 1501 ITLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560

Query: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFSH 1620
            PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFSH
Sbjct: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFSH 1620

Query: 1621 ILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPVVRQQIIDGFTGKA 1680
            ILIWH              LQGETCEPETGKEATSTK NSFQALLPVVRQ IIDGF+GKA
Sbjct: 1621 ILIWH--------------LQGETCEPETGKEATSTKTNSFQALLPVVRQHIIDGFSGKA 1680

Query: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVKGI 1740
            LDVFQREFDFFDKVTSISGVLFPLPKDERRAGI+RELEKIEMNGDDLYLPTAPSKLVKGI
Sbjct: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMNGDDLYLPTAPSKLVKGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRD 1800
            QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVISLLRD 1800

Query: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRF 1920
            SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGI+TTVALMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGIITTVALMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2022

BLAST of HG10000440 vs. NCBI nr
Match: XP_038902922.1 (phosphatidylinositol 4-kinase alpha 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 3808.1 bits (9874), Expect = 0.0e+00
Identity = 1934/2037 (94.94%), Postives = 1965/2037 (96.47%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNA+LA+ARFLSKC
Sbjct: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAILAVARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA
Sbjct: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            +VAVYTGEVVASAISSGGGD GISRSFLMVFSKHFLPILPSDANKLVDMVIERYLM GS 
Sbjct: 121  DVAVYTGEVVASAISSGGGDLGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRN-GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240
            APRE MQGNSDSSA  SIDGSSPRN GSHDSGSRVGDDNISSWKS+ATSYGSGILWKSS+
Sbjct: 181  APRELMQGNSDSSASHSIDGSSPRNEGSHDSGSRVGDDNISSWKSTATSYGSGILWKSSS 240

Query: 241  DQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300
            DQLGPMFG NDGGGGGE+LLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD
Sbjct: 241  DQLGPMFGLNDGGGGGEILLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300

Query: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360
            QVR+IAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVY+AAAKLKLKSLGSLDSEG
Sbjct: 301  QVRAIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYKAAAKLKLKSLGSLDSEG 360

Query: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420
            KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI
Sbjct: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420

Query: 421  RLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGR 480
            RLKPLVLTVCAQTDPSDS H  MFETVSKT+CEI+ESCWTKERAPVDTFIMGLTASIRGR
Sbjct: 421  RLKPLVLTVCAQTDPSDSSHGGMFETVSKTMCEIIESCWTKERAPVDTFIMGLTASIRGR 480

Query: 481  NDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540
            NDYEEQVN+EKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR
Sbjct: 481  NDYEEQVNREKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540

Query: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600
            LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP
Sbjct: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600

Query: 601  AGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660
             GFLLIASGL SSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF
Sbjct: 601  TGFLLIASGLTSSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660

Query: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720
            SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG
Sbjct: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720

Query: 721  PYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780
            PYMWDK+WSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA
Sbjct: 721  PYMWDKRWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780

Query: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVF 840
            LATALGGRVDISAM+TISGVKATYLLAVSFLEITRFSSNGGILNGSI+VDASRSAFSCVF
Sbjct: 781  LATALGGRVDISAMNTISGVKATYLLAVSFLEITRFSSNGGILNGSINVDASRSAFSCVF 840

Query: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSM 900
            EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAA+RNSILNAHTCFLIKSM
Sbjct: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAVRNSILNAHTCFLIKSM 900

Query: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSL 960
            CQREDHIREVAVTLLTQFRDKFP                                     
Sbjct: 901  CQREDHIREVAVTLLTQFRDKFP------------------------------------- 960

Query: 961  YQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020
             QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW
Sbjct: 961  -QKVVREWIIRSLSHAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020

Query: 1021 TGI-QTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN 1080
            +GI QTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN
Sbjct: 1021 SGILQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYN 1080

Query: 1081 TFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAE 1140
            TFGGYQSAGA PTGSGIGIGLQRLITGAFPQ+PQGEDESFNAMLLLKFVQSLQKFVNNAE
Sbjct: 1081 TFGGYQSAGAAPTGSGIGIGLQRLITGAFPQDPQGEDESFNAMLLLKFVQSLQKFVNNAE 1140

Query: 1141 TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV
Sbjct: 1141 TGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGCV+LAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM
Sbjct: 1201 FVWTWLVSAAPQLGCVILAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQSQVNLQNFK+GLQLLEDRVYRASLGWFAFEPEWH+INSTNFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKSGLQLLEDRVYRASLGWFAFEPEWHEINSTNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQ+ENYTVGREKR+QL
Sbjct: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQMENYTVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NVTLRAEVSQQVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL
Sbjct: 1501 NVTLRAEVSQQVQSHILDIRYIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSD+F+
Sbjct: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDLFA 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPVVRQQIIDGFTGK 1680
            HILIWH              LQGETCEPE GKEATSTK+NSFQALLPVVRQ IIDGF+ K
Sbjct: 1621 HILIWH--------------LQGETCEPEMGKEATSTKSNSFQALLPVVRQHIIDGFSEK 1680

Query: 1681 ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVKG 1740
            ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGI+RELEKIEMNGDDLYLPTAPSKLVKG
Sbjct: 1681 ALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMNGDDLYLPTAPSKLVKG 1740

Query: 1741 IQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR 1800
            IQVDSGIPLQSAAKVPIMITFNV DRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR
Sbjct: 1741 IQVDSGIPLQSAAKVPIMITFNVVDRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLR 1800

Query: 1801 DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS 1860
            DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS
Sbjct: 1801 DVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGS 1860

Query: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMR 1920
            PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNMR
Sbjct: 1861 PSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNMR 1920

Query: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSGL 1980
            FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGI+TTVALMLDSGL
Sbjct: 1921 FESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGIITTVALMLDSGL 1980

Query: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 PCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 1985

BLAST of HG10000440 vs. NCBI nr
Match: XP_022947643.1 (phosphatidylinositol 4-kinase alpha 1-like isoform X1 [Cucurbita moschata] >KAG6605090.1 Phosphatidylinositol 4-kinase alpha 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035097.1 Phosphatidylinositol 4-kinase alpha 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3691.7 bits (9572), Expect = 0.0e+00
Identity = 1859/2038 (91.22%), Postives = 1940/2038 (95.19%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCDLIA+NP+AF+EK++WICSRCPPPDALL++SPRVSRSQLNA+LAIARFLSKC
Sbjct: 1    MEALFELCDLIAENPTAFYEKLSWICSRCPPPDALLAESPRVSRSQLNAILAIARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            AD  E RPKAVVLEFIRSIPSSF+ SFWP TYGNDSITLFYTEFL YVS+A EITPDFSA
Sbjct: 61   ADCFELRPKAVVLEFIRSIPSSFHPSFWPQTYGNDSITLFYTEFLRYVSRAAEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISS  GDS ISRSFLMV SKHF P+LPSDANK+V+MVIER+L+ GS 
Sbjct: 121  EVAVYTGEVVASAISSSAGDSSISRSFLMVLSKHFFPVLPSDANKIVEMVIERFLLTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRNGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSAD 240
            APRE MQGNSDSSA  SI+GSSP+NG        G+DNISSWKSSA+SYGSG+LWKSSAD
Sbjct: 181  APREHMQGNSDSSAFHSIEGSSPQNG--------GEDNISSWKSSASSYGSGVLWKSSAD 240

Query: 241  QLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWDQ 300
            QLG MFGFNDGGGGGEVLLKQQV+ FEEESLE +EKQAIAFELLAHILDKARIDS LWDQ
Sbjct: 241  QLGSMFGFNDGGGGGEVLLKQQVAIFEEESLECVEKQAIAFELLAHILDKARIDSSLWDQ 300

Query: 301  VRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEGK 360
             RSIAKKQLQSLLIFVKIRKRDWNEQGS+LKARINAKLLVYQAAAKLKLKSLGSLD+EGK
Sbjct: 301  ARSIAKKQLQSLLIFVKIRKRDWNEQGSVLKARINAKLLVYQAAAKLKLKSLGSLDNEGK 360

Query: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLIR 420
            VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLG+IAVAWKGQPLRVLLIR
Sbjct: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGQIAVAWKGQPLRVLLIR 420

Query: 421  LKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRN 480
            LKPLVLTVCAQTDP+DS  N MFETVSKT+CEIVESCWTKERAPVDTFIMGLTASIRGRN
Sbjct: 421  LKPLVLTVCAQTDPTDSSQNTMFETVSKTMCEIVESCWTKERAPVDTFIMGLTASIRGRN 480

Query: 481  DYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRL 540
            DYEEQVNKE+ VPPLQLNGIRLLANLNVAVKK EIVDMILPLFIESLEEGDAS+PC+LRL
Sbjct: 481  DYEEQVNKEREVPPLQLNGIRLLANLNVAVKKPEIVDMILPLFIESLEEGDASTPCVLRL 540

Query: 541  QFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLPA 600
            QFLDA+SRMASLGFEKSYRETVVLMTRSYLSKLSNAAS PPE     PEATTERLETLPA
Sbjct: 541  QFLDAVSRMASLGFEKSYRETVVLMTRSYLSKLSNAASAPPESGGGTPEATTERLETLPA 600

Query: 601  GFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDFS 660
            GF LIASGLA+ KLRSDYRYRLLSLCSDVGLAADSRN+RSGADFLGPLLPAVAEICSDFS
Sbjct: 601  GFFLIASGLANPKLRSDYRYRLLSLCSDVGLAADSRNSRSGADFLGPLLPAVAEICSDFS 660

Query: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGP 720
            PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNS++PSKAISTTVS+AGS+GTTAIQAVSGP
Sbjct: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSALPSKAISTTVSTAGSIGTTAIQAVSGP 720

Query: 721  YMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAAL 780
            YMWDKQWS+AVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTAN+KVVLAQRAAL
Sbjct: 721  YMWDKQWSAAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANDKVVLAQRAAL 780

Query: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVFE 840
            ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSA SCVFE
Sbjct: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAISCVFE 840

Query: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSMC 900
            YLKTPNLVPAVLQCLTALVHRAYETAVSWLE RIS+IGNEAA+RNSILNAHTCFLIKSMC
Sbjct: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEHRISDIGNEAAVRNSILNAHTCFLIKSMC 900

Query: 901  QREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSLY 960
            QREDHIREVAVTLLTQFRD+FPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSLY
Sbjct: 901  QREDHIREVAVTLLTQFRDRFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSLY 960

Query: 961  QKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCWT 1020
            QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDV SLLSEIRIGTGKN+CWT
Sbjct: 961  QKVVREWIIRSLSFAPCTSQGLLQEMLCKANTWQRAQHTPDVVSLLSEIRIGTGKNECWT 1020

Query: 1021 GIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080
            G+QTANIPAVM+AAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF
Sbjct: 1021 GVQTANIPAVMAAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080

Query: 1081 GGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQ-GEDESFNAMLLLKFVQSLQKFVNNAET 1140
            GGYQS G  P GSGIGIGLQRLITGAFPQEPQ  EDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 GGYQSTGTAPAGSGIGIGLQRLITGAFPQEPQAAEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANL-GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            GGEIDKSQFHETCSQATAFLLANL   DSKSNIEGLSQLLRLLCWCPAYIS SNAMETGV
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLDNADSKSNIEGLSQLLRLLCWCPAYISRSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGC+VLAELVDAWLWTIDTKRGLFSS+EA SGPTAKLRPHLYPGDPE+
Sbjct: 1201 FVWTWLVSAAPQLGCIVLAELVDAWLWTIDTKRGLFSSKEALSGPTAKLRPHLYPGDPEL 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPE DPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEFDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQ+QVNL+NF+TGL+LLEDRVYRASLGWF+FEPEWHDINS+NFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQTQVNLRNFETGLRLLEDRVYRASLGWFSFEPEWHDINSSNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALF QYLSN LKDGSQIDVKGQVRENGNS D + CHPVWG ++NY VGREKR+QL
Sbjct: 1381 EAQSVALFAQYLSNSLKDGSQIDVKGQVRENGNSADQSFCHPVWGPMDNYAVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWA PVNTKETTS RPKISSEKW+EYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWANPVNTKETTSLRPKISSEKWIEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NV LR+EVS QVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSI QAIEF+
Sbjct: 1501 NVALRSEVSHQVQSHILDIRSIPEALPYFVSPKAVDENSVLLQQLPHWAACSIVQAIEFI 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            AP+YKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR+DDGKLVEGYLLRAAQRSDIFS
Sbjct: 1561 APSYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRHDDGKLVEGYLLRAAQRSDIFS 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPET-GKEATSTKNNSFQALLPVVRQQIIDGFTG 1680
            HILIWH              LQGETCEPET GK+AT  K ++FQALLPVVRQ+IIDGF+ 
Sbjct: 1621 HILIWH--------------LQGETCEPETAGKDATVGKMSAFQALLPVVRQKIIDGFSP 1680

Query: 1681 KALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVK 1740
             A D+FQREFDFFDKVTSISGVLFP+PKDERRAGI++ELEKIEM+GDDLYLPTAP KLV+
Sbjct: 1681 SARDIFQREFDFFDKVTSISGVLFPIPKDERRAGIRKELEKIEMDGDDLYLPTAPGKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNV DRDGD ND+KPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVIDRDGDHNDIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDVFEAVGL+LYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDVFEAVGLSLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNM 1920
            SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYL+ARRYMDGILTTVALMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLSARRYMDGILTTVALMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2016

BLAST of HG10000440 vs. ExPASy Swiss-Prot
Match: Q9SXA1 (Phosphatidylinositol 4-kinase alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KA1 PE=1 SV=2)

HSP 1 Score: 2902.5 bits (7523), Expect = 0.0e+00
Identity = 1465/2055 (71.29%), Postives = 1714/2055 (83.41%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCD+IA+NP  F EK+AWIC RCP  + LL++SPRVSRS LNAVLA+AR +SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
             +S++ R K+VV EF+ +IP+SF  SFWP ++ +  I+ FY +FL Y+S A +++P+F  
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVAVYTGEVVASAISSGGGDS----GISRSFLMVFSKHFLPILPSDANKLVDMVIERYLM 180
            EVA +TGEVV +AI+   GDS     IS++FL+  S+HF  IL SD +KL+ M+++++++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  -MGSGAPREQMQGNSDSSA--PQSIDGSSPRNGSHDSG----SRVGDD------NISSWK 240
                 +P+EQ Q NS +S     S  GS      + SG    +  GD+      N+SS  
Sbjct: 181  NRAPASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSSKS 240

Query: 241  SSA-TSYGSGILWKSSADQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFE 300
            SS+    G  I+WKS  DQL   FGF++G GG   + +QQV+SFE+ES+ESLEKQ IAF 
Sbjct: 241  SSSVVMNGGSIVWKSGVDQLS--FGFSEGSGGANPVFRQQVASFEDESIESLEKQEIAFR 300

Query: 301  LLAHILDKARIDSWLWDQVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQ 360
            L+ HILDK +IDS L DQVR IAK+QLQS+  F+K RKRDWNEQG +LK R+NAKL VYQ
Sbjct: 301  LITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLKTRVNAKLSVYQ 360

Query: 361  AAAKLKLKSLGSLDSEGKVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVL 420
            AAAK+K+KSL SL+++GK +K+LV ET ALL+DAA+AC+ S+WRK++ CEELF  LL+ +
Sbjct: 361  AAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKACEELFDSLLSGI 420

Query: 421  GKIAVAWKGQPLRVLLIRLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKER 480
             KIAVA  GQPLRVLLIRLKPLVL VCA  D       AM E++ KT C I+ES W K+R
Sbjct: 421  AKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSCVIIESAWAKDR 480

Query: 481  APVDTFIMGLTASIRGRNDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPL 540
            APVD FIMGL +SIR RNDYEEQV++EK VP +QLN IRLLA+LNVAVKK E+ DMILPL
Sbjct: 481  APVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVKKPEVADMILPL 540

Query: 541  FIESLEEGDASSPCLLRLQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE 600
            FIESLEEGDAS+P  LRLQ LDA+SR+A+LGF+KSYRETVVLMTRSYLSKLS+  S   E
Sbjct: 541  FIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLSKLSSVGS--VE 600

Query: 601  GSAPAPEATTERLETLPAGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGA 660
                APEATTER+ETLPAGFL IASGL  +KLRSDYR+RLLSLCSDVGLAA+S++  SG 
Sbjct: 601  SKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAAESKSGGSGV 660

Query: 661  DFLGPLLPAVAEICSDFSPTGDVEPSLLKLFRNLWFYVALFGLAPPI-QNSSVPSKAIST 720
            DFLGPLLPAVAEICSDF PT DVEPSLLKLFRNLWFY+ALFGLAPPI +  + P K+ S 
Sbjct: 661  DFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTPTPPLKSTSN 720

Query: 721  TVSSAGSVGTTAIQAVSGPYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNP 780
            +V+S GS+  TA+QAV GPYMWD QW+ AVQRI+QGTPPLVVS+VKWLEDELELNAL NP
Sbjct: 721  SVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDELELNALHNP 780

Query: 781  VSRQGTANEKVVLAQRAALATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGI 840
             SR+G  NEKV   QR AL+TALGGRVD++AM+TISGVKATYLLAV+FLEI RF SNGGI
Sbjct: 781  GSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEIIRFISNGGI 840

Query: 841  LNGSISVDASRSAFSCVFEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEA 900
            LNG  SV ASRSAFSCVFEYLKTPNL PAV QCLTA+VHRA+ETAVSWLE RIS  G +A
Sbjct: 841  LNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLEDRISLTGKDA 900

Query: 901  AIRNSILNAHTCFLIKSMCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQ 960
              R     AH CFLIKSM QR++H+R+++V LLTQ RDKFPQV+W+SSCLDSLLFS+H+ 
Sbjct: 901  RNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLDSLLFSVHDN 960

Query: 961  SPTTIVNDPAWVVTVRSLYQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPD 1020
            +P+T+VNDPAW   VRSLYQKVVREWII SLSYAPCTSQGLLQ+ LCKANTWQRAQ T D
Sbjct: 961  TPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANTWQRAQTTTD 1020

Query: 1021 VASLLSEIRIGTGKNDCWTGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSAT 1080
            V SLLSEI+IGTGKN+ W+GI+TANIPAVM+AAAAASG NL +++AFNLEVL TG+ SAT
Sbjct: 1021 VVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEVLGTGVVSAT 1080

Query: 1081 VKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAM 1140
            VKCN+ GEIAGMRRLYN+ GG+QS     T SG G GLQRLI+GAF Q PQ ED+SFN M
Sbjct: 1081 VKCNHAGEIAGMRRLYNSIGGFQSGS---TPSGFGGGLQRLISGAFSQAPQPEDDSFNEM 1140

Query: 1141 LLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLL 1200
            L+ +FV+ LQ+FVN AE GGE++KSQF ETCSQATA LL+NLG +SK+N+EG SQLLRLL
Sbjct: 1141 LIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEGFSQLLRLL 1200

Query: 1201 CWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFS 1260
            CWCPAYIST +AMETG+F+WTWLVSAAPQL  +VLAELVDAW+WTIDTKRGLF+S   +S
Sbjct: 1201 CWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGLFASDVRYS 1260

Query: 1261 GPTAKLRPHLYPGDPEMPPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQ 1320
            GP AKLRPHL PG+PE PPE DPV+QI+AHRLWLGF  DRFEVVR+ S EQLLL GR+LQ
Sbjct: 1261 GPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQLLLLGRMLQ 1320

Query: 1321 GSMELPWKFSHHPAATGSFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWF 1380
             S +L W F+ HPAA G+FF+LM LGLKFCSCQ+Q N+Q F++GLQLLEDR+YR SLGWF
Sbjct: 1321 RSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRIYRTSLGWF 1380

Query: 1381 AFEPEWHDINSTNFAQSEAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDL-NSCHP 1440
            A +PEW+D+N  NF  SEA SV++FV +LSN L + SQ D KG+ RE+GN +D+ +  HP
Sbjct: 1381 AHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESGNLIDVTDQYHP 1440

Query: 1441 VWGQIENYTVGREKRKQLLLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYART 1500
            VWG+++NYT+G+EKRKQLLLMLCQHEADRL+VWAQP+++K++  SR KISSEKW EYA+T
Sbjct: 1441 VWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISSEKWTEYAKT 1500

Query: 1501 AYSVDPRIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLL 1560
            A+SVDPRIA S+ SRFPAN ++++EV+Q VQ++I+D+R IPEALPYFV+PK V+ENSVLL
Sbjct: 1501 AFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPKNVEENSVLL 1560

Query: 1561 QQLPHWAACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDG 1620
            QQLPHWAACSITQA+EFL PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQ+LRYDDG
Sbjct: 1561 QQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQSLRYDDG 1620

Query: 1621 KLVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSF 1680
            +LVEGYLLRA QRSDIF+HILIWH              LQGE  + ET K+ +  KN +F
Sbjct: 1621 RLVEGYLLRATQRSDIFAHILIWH--------------LQGEDVQ-ETPKDGSIDKNAAF 1680

Query: 1681 QALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIE 1740
            Q +LP VRQ IIDGF+  ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGI+RELEKIE
Sbjct: 1681 QEILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIE 1740

Query: 1741 MNGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKV 1800
            M GDDLYLPTAP+KLV+GI+VDSGIPLQSAAKVPIMITFNV DRDGD +DVKPQACIFKV
Sbjct: 1741 MQGDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKV 1800

Query: 1801 GDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGET 1860
            GDDCRQDVLALQVI+LLRD+F+A GLNLYLFPYGVLPTG ERGIIEVVPN RSRSQMGET
Sbjct: 1801 GDDCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGET 1860

Query: 1861 TDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRL 1920
            TDGGLYEIFQQDYGPVGS +FE ARENF+ISSAGYAVASLLLQPKDRHNGNLLFDD+GRL
Sbjct: 1861 TDGGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRL 1920

Query: 1921 IHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAAR 1980
            +HIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ F+SLCVKGYLAAR
Sbjct: 1921 VHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAAR 1980

Query: 1981 RYMDGILTTVALMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTT 2036
            R MDGI++TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VC DAYNKWTT
Sbjct: 1981 RQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTT 2028

BLAST of HG10000440 vs. ExPASy Swiss-Prot
Match: Q9C680 (Phosphatidylinositol 4-kinase alpha 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KA2 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 2.0e-196
Identity = 344/434 (79.26%), Postives = 375/434 (86.41%), Query Frame = 0

Query: 1602 LVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQ 1661
            +VEGYLLRAAQRSDIF+H LIWH              LQGE+ E E  K+    KN SFQ
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWH--------------LQGESVE-EIVKDGAFDKNASFQ 174

Query: 1662 ALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEM 1721
             +L  VRQ I+DGFT KAL+ F REFDFF+KVTSISG L PLPK+ER AGI+RELEKI+M
Sbjct: 175  EILSDVRQHIVDGFTPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKM 234

Query: 1722 NGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVG 1781
             G+DLYLPTAP+KLV+ IQVDSGIPLQSAAKVPIMITFNV D DGD NDVKPQACIFKVG
Sbjct: 235  QGEDLYLPTAPNKLVRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVG 294

Query: 1782 DDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETT 1841
            DDCRQDVLALQVI+LL D+F+AVGLNLYLFPYGVLPT        VVPN RSRSQMGETT
Sbjct: 295  DDCRQDVLALQVISLLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETT 354

Query: 1842 DGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLI 1901
            DGGLYEIFQQ+YG VGS +FE AR NF+ISSAGYAVASLLLQPKDRHNGNLLFDD+GRL+
Sbjct: 355  DGGLYEIFQQNYGLVGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLV 414

Query: 1902 HIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARR 1961
            HIDFGFILETSPGGNMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ F+SLCVKGYLAARR
Sbjct: 415  HIDFGFILETSPGGNMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARR 474

Query: 1962 YMDGILTTVALMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTA 2021
            YMD I++TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA FMI VC DAYNKWTTA
Sbjct: 475  YMDEIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTA 525

Query: 2022 GYDLIQYLQQGIEK 2036
            GYDLIQYLQQG+EK
Sbjct: 535  GYDLIQYLQQGVEK 525

HSP 2 Score: 125.2 bits (313), Expect = 8.6e-27
Identity = 56/114 (49.12%), Postives = 79/114 (69.30%), Query Frame = 0

Query: 1   MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
           ME L ELCD+I QNP  F +K+ WIC +CP    L ++SPR S+S LNA+LA+ R LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEI 115
            D+ +   K VVL+F++++P SF+ SFWP +   +SI+ FY  FLGYVS  +++
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQL 114

BLAST of HG10000440 vs. ExPASy Swiss-Prot
Match: O02811 (Phosphatidylinositol 4-kinase alpha OS=Bos taurus OX=9913 GN=PI4KA PE=2 SV=2)

HSP 1 Score: 578.9 bits (1491), Expect = 2.2e-163
Identity = 459/1721 (26.67%), Postives = 750/1721 (43.58%), Query Frame = 0

Query: 426  LTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRNDYEEQ 485
            L V +      S++  + +     +C  +++  T +   V+ F+    AS+  R    ++
Sbjct: 549  LNVASGKKSQPSMYEQLRDIAIDNVCRCLKAGLTVDPVIVEAFL----ASLSNRLYISQE 608

Query: 486  VNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRLQFLDA 545
             +K+  + P   + IR L ++ VA++ +     ++   ++ L++     P  L +  +D 
Sbjct: 609  SDKDAHLIP--DHTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQ 668

Query: 546  ISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE----GSAPAPEATTERLETLPAG 605
            +  +   G +  Y+E   L  +  +   S   S   +    G      A    L      
Sbjct: 669  LGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALAN---- 728

Query: 606  FLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNAR-----------SGADFLGPLLP 665
               IA+ +    L  +    LL L   +GL     + R           S A  LG L+P
Sbjct: 729  ---IAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIP 788

Query: 666  AVAEICSDFSPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVG 725
             +A +     P  + +P L KLFR+ W Y  L G                          
Sbjct: 789  VIAVLTRRLPPIKEPKPRLQKLFRDFWLYSVLMGF------------------------- 848

Query: 726  TTAIQAVSGPYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANE 785
                 AV G  +W ++W   V  I+  +P L   + + L   L+ N+      +  T   
Sbjct: 849  -----AVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS----AMKNDTVTP 908

Query: 786  KVVLAQRAALATALGGRVDISAM-STISGVKATYLLAVSFLEITRFSSNGGILNGSISVD 845
              +   R+ +   L    ++SA+   +    +TYLL+V  LE  R      +L  S   D
Sbjct: 909  AELSELRSTIVNLLDPAPEVSALIGKLDFAMSTYLLSVYRLEYMR------VLRSS---D 968

Query: 846  ASR-SAFSCVFEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSIL 905
             +R     C FE          ++QC+ A+  + ++  ++ + ++     NE       L
Sbjct: 969  PARFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----L 1028

Query: 906  NAHTCFLIKSMCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLL-----------FS 965
              H  FL+ S       IR VA   L+   DKFP ++W+ + L ++L             
Sbjct: 1029 ERHAQFLLVSFNHVHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSAD 1088

Query: 966  MHNQSPTTIVNDPAWVVTVRSLY---QKVVREWIIR-------SLSYAPCTSQGLLQEML 1025
            +H   P   + D  + +TV   Y   + +V+++  R       ++ +AP  ++  LQE L
Sbjct: 1089 IHKDQPYYDIPDVPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYL 1148

Query: 1026 CKANTWQR--AQHTPDVASLLSEIRIGTGKNDCWTGI---QTANIPAVMSAAAAASGGNL 1085
             K   W    +QHT  +A     +    G N   T +   Q    PA +    +    +L
Sbjct: 1149 SKHQNWVSGLSQHT-GLAMATESVLHYAGYNKQSTSLGATQLTERPACVKKDYSNFMASL 1208

Query: 1086 NLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRL 1145
            NL                  +  Y GE+ GM R                           
Sbjct: 1209 NL------------------RNRYAGEVYGMIRF-------------------------- 1268

Query: 1146 ITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLAN 1205
                   +  G     N M++ +   +L          G  D  Q+ +   + TA L+++
Sbjct: 1269 ------SDATGHTSDLNKMMVQELKAAL----------GAGDAQQYTQAMFKLTAMLISS 1328

Query: 1206 LGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDA 1265
               D         QLL  LCW P  +   + MET +  W WL++    +    + E+  A
Sbjct: 1329 RDCD--------PQLLHHLCWGPLQMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGA 1388

Query: 1266 WLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMPPEIDPVEQIIAHRLWLGFFFDRF 1325
            W  T++ K GLFS+    + P A         +   P    P  ++  H +W+ F   RF
Sbjct: 1389 WQMTVEQKFGLFSAEMKEADPLA-------ASEASQPKPCAP--EVTPHYIWIDFLVQRF 1448

Query: 1326 EVVRYQSVEQLLLFGRLLQGSMEL-----PWKFSHHPAATGSFFTLMFLGLKFCSCQSQV 1385
            E+ +Y S +Q+ +F  LLQ S+ L         + H AA G  F L+ LGL         
Sbjct: 1449 EIAKYCSSDQVEIFCSLLQRSLSLSIGGTAGSMNRHVAAIGPRFKLLTLGLSLLHADVLP 1508

Query: 1386 NLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSEAQSVALFVQYLSN--YLK 1445
            N     T   +L +++Y  +  +F+  P +         +  +  +  +    S+  YL 
Sbjct: 1509 N----ATIRNVLREKIYSTAFDYFSCPPRFPTQGEKRLREDISVMIKFWTAMFSDKKYLT 1568

Query: 1446 DGSQIDVKGQVRENGNSVDLNS-CHPVWGQIENY-------TVGRE-------------- 1505
                +    Q   +   + + S      G I  Y       T+ ++              
Sbjct: 1569 ASQLVPPDNQDTRSNLDIAVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLH 1628

Query: 1506 ----KRKQLLLMLCQHEADRLEVWAQPVNTKET-------------TSSRPKISSEKWVE 1565
                KR+ LLL L   E +RL  W  P++  E               S    +S ++W +
Sbjct: 1629 KYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGESSVANWRSKYISLSEKQWKD 1688

Query: 1566 YARTAYSVDPRIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDEN 1625
                A+S+ P +A  L +RF     +  EV++ V+     +  +PEA+ + V+   +D +
Sbjct: 1689 NVNLAWSISPHLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDAD 1748

Query: 1626 SVLLQQLPHWAACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR 1685
            +  L  +  WA       + + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALR
Sbjct: 1749 APELSHVLCWAPADPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALR 1808

Query: 1686 YDDGKLVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTK 1745
            YD    V  Y+L AA +S + +H  IW+++        +  D +G   +P+ G       
Sbjct: 1809 YDKMGYVREYILWAASQSQLLAHQFIWNMK------TNIYVDEEGHQKDPDIGD------ 1868

Query: 1746 NNSFQALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPK-DERRAGIQRE 1805
                  LL  + ++I    +G A D +QREFDFF+K+T++S ++ P PK DER+      
Sbjct: 1869 ------LLEQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSA 1928

Query: 1806 LEKIEMNGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNV-------------- 1865
            L ++++     YLP+ P  +V  I   SG P+QSAAK P +  F V              
Sbjct: 1929 LSEVKVQ-PGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLR 1988

Query: 1866 ---------ADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRDVFEAVGLNLYLFP 1925
                     + ++ D   +  QA IFKVGDDCRQD+LALQ+I L +++F+ VGL+L++FP
Sbjct: 1989 CRSDPEEEGSMQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFP 2048

Query: 1926 YGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSPSFEAARENFIISS 1985
            Y V+ T P  G+IE +P+  SR Q+G  TD G+Y+ F + YG   + +F+ AR NFI S 
Sbjct: 2049 YRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSM 2100

Query: 1986 AGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRFESAHFKLSHEMTQ 2034
            A Y++   LLQ KDRHNGN++ D  G LIHIDFGF+ E+SPGGN+ +E    KL+ EM  
Sbjct: 2109 AAYSLLLFLLQIKDRHNGNIMLDKKGHLIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVM 2100

BLAST of HG10000440 vs. ExPASy Swiss-Prot
Match: P42356 (Phosphatidylinositol 4-kinase alpha OS=Homo sapiens OX=9606 GN=PI4KA PE=1 SV=4)

HSP 1 Score: 575.9 bits (1483), Expect = 1.8e-162
Identity = 461/1721 (26.79%), Postives = 752/1721 (43.70%), Query Frame = 0

Query: 426  LTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRNDYEEQ 485
            L V +      S++  + +     +C  +++  T +   V+ F+    AS+  R    ++
Sbjct: 549  LNVMSGKKSQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQE 608

Query: 486  VNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRLQFLDA 545
             +K+  + P   + IR L ++ VA++ +     ++   ++ L++     P  L +  +D 
Sbjct: 609  SDKDAHLIP--DHTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQ 668

Query: 546  ISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE----GSAPAPEATTERLETLPAG 605
            +  +   G +  Y+E   L  +  +   S   S   +    G      A    L      
Sbjct: 669  LGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALAN---- 728

Query: 606  FLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNAR-----------SGADFLGPLLP 665
               IA+ +    L  +    LL L   +GL     + R           S A  LG L+P
Sbjct: 729  ---IAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIP 788

Query: 666  AVAEICSDFSPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVG 725
             +A +     P  + +P L KLFR+ W Y  L G                          
Sbjct: 789  VIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGF------------------------- 848

Query: 726  TTAIQAVSGPYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANE 785
                 AV G  +W ++W   V  I+  +P L   + + L   L+ N+      +  T   
Sbjct: 849  -----AVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNS----AMKNDTVTP 908

Query: 786  KVVLAQRAALATALGGRVDISAM-STISGVKATYLLAVSFLEITRFSSNGGILNGSISVD 845
              +   R+ +   L    ++SA+ + +    +TYLL+V  LE  R      +L    S D
Sbjct: 909  AELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMR------VLR---STD 968

Query: 846  ASR-SAFSCVFEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSIL 905
              R     C FE          ++QC+ A+  + ++  ++ +  +     NE       L
Sbjct: 969  PDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEE-----L 1028

Query: 906  NAHTCFLIKSMCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLL-----------FS 965
              H  FL+ +       IR VA   L+   DKFP ++W+ + L ++L             
Sbjct: 1029 ERHAQFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSAD 1088

Query: 966  MHNQSPTTIVNDPAWVVTVRSLY---QKVVREWIIR-------SLSYAPCTSQGLLQEML 1025
            +H   P   + D  + +TV   Y   + +V+++  R       ++ +AP  ++  LQE L
Sbjct: 1089 IHKDQPYYDIPDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYL 1148

Query: 1026 CKANTWQR--AQHTPDVASLLSEIRI-GTGKNDCWTG-IQTANIPAVMSAAAAASGGNLN 1085
             K   W    +QHT    +  S +   G  K +   G  Q +  PA +    +    +LN
Sbjct: 1149 NKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTLGATQLSERPACVKKDYSNFMASLN 1208

Query: 1086 LTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRLI 1145
            L                  +  Y GE+ GM R   T                        
Sbjct: 1209 L------------------RNRYAGEVYGMIRFSGT------------------------ 1268

Query: 1146 TGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLANL 1205
                     G+    N M+    VQ L   ++ +          + +   + TA L+++ 
Sbjct: 1269 --------TGQMSDLNKMM----VQDLHSALDRSH------PQHYTQAMFKLTAMLISSK 1328

Query: 1206 GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDAW 1265
              D         QLL  LCW P  +   + MET +  W WL++    +    + E+  AW
Sbjct: 1329 DCD--------PQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAW 1388

Query: 1266 LWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPE-MPPEIDPVEQIIAHRLWLGFFFDRF 1325
              T++ K GLFS+    + P A          P+  PPE+ P      H +W+ F   RF
Sbjct: 1389 HMTVEQKFGLFSAEIKEADPLAASE----ASQPKPCPPEVTP------HYIWIDFLVQRF 1448

Query: 1326 EVVRYQSVEQLLLFGRLLQGSMEL-----PWKFSHHPAATGSFFTLMFLGLKFCSCQSQV 1385
            E+ +Y S +Q+ +F  LLQ SM L         + H AA G  F L+ LGL         
Sbjct: 1449 EIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVP 1508

Query: 1386 NLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSEAQSVALFVQYLSN--YLK 1445
            N     T   +L +++Y  +  +F+  P++         +  +  +  +    S+  YL 
Sbjct: 1509 N----ATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLT 1568

Query: 1446 DGSQIDVKGQVRENGNSVDLNS-CHPVWGQIENY-------TVGRE-------------- 1505
                +    Q   +   + + S      G I  Y       T+ ++              
Sbjct: 1569 ASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLH 1628

Query: 1506 ----KRKQLLLMLCQHEADRLEVWAQPVNTKET-------------TSSRPKISSEKWVE 1565
                KR+ LLL L   E +RL  W  P++  E               S    +S ++W +
Sbjct: 1629 KYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKD 1688

Query: 1566 YARTAYSVDPRIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDEN 1625
                A+S+ P +A  L +RF     +  EV++ V+     +  +PEA+ + V+   +D +
Sbjct: 1689 NVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDAD 1748

Query: 1626 SVLLQQLPHWAACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR 1685
            +  L  +  WA       + + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALR
Sbjct: 1749 APELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALR 1808

Query: 1686 YDDGKLVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTK 1745
            YD    V  Y+L AA +S + +H  IW+++        +  D +G   +P+ G       
Sbjct: 1809 YDKMGYVREYILWAASKSQLLAHQFIWNMK------TNIYLDEEGHQKDPDIGD------ 1868

Query: 1746 NNSFQALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPK-DERRAGIQRE 1805
                  LL  + ++I    +G A D +QREFDFF+K+T++S ++ P PK DER+      
Sbjct: 1869 ------LLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSA 1928

Query: 1806 LEKIEMNGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNV-------------- 1865
            L ++++     YLP+ P  +V  I   SG P+QSAAK P +  F V              
Sbjct: 1929 LSEVKVQ-PGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLR 1988

Query: 1866 ---------ADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRDVFEAVGLNLYLFP 1925
                     + ++ D   +  QA IFKVGDDCRQD+LALQ+I L +++F+ VGL+L++FP
Sbjct: 1989 CRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFP 2048

Query: 1926 YGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSPSFEAARENFIISS 1985
            Y V+ T P  G+IE +P+  SR Q+G  TD G+Y+ F + YG   + +F+ AR NFI S 
Sbjct: 2049 YRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSM 2100

Query: 1986 AGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRFESAHFKLSHEMTQ 2034
            A Y++   LLQ KDRHNGN++ D  G +IHIDFGF+ E+SPGGN+ +E    KL+ EM  
Sbjct: 2109 AAYSLLLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVM 2100

BLAST of HG10000440 vs. ExPASy Swiss-Prot
Match: O08662 (Phosphatidylinositol 4-kinase alpha OS=Rattus norvegicus OX=10116 GN=Pi4ka PE=1 SV=2)

HSP 1 Score: 573.2 bits (1476), Expect = 1.2e-161
Identity = 462/1711 (27.00%), Postives = 750/1711 (43.83%), Query Frame = 0

Query: 437  SIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRNDYEEQVNKEKGVPPLQ 496
            S++  + +     +C  +++  T +   V+ F+    AS+  R    ++ +K+  + P  
Sbjct: 554  SMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFL----ASLSNRLYISQESDKDAHLIP-- 613

Query: 497  LNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRLQFLDAISRMASLGFEK 556
             + IR L ++ VA++ +     ++   ++ L++     P  L +  +D +  +   G + 
Sbjct: 614  DHTIRALGHIAVALRDT---PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQY 673

Query: 557  SYRETVVLMTRSYLSKLSNAASPPPE----GSAPAPEATTERLETLPAGFLLIASGLASS 616
             Y+E   L  +  +   S   S   +    G      A    L         IA+ +   
Sbjct: 674  IYQEVWNLFQQISVKASSVVYSATKDYKDHGYRHCSLAVINALAN-------IAANIQEE 733

Query: 617  KLRSDYRYRLLSLCSDVGLAADSRNAR-----------SGADFLGPLLPAVAEICSDFSP 676
             L  +    LL L   +GL     + R           S A  LG L+P +A +     P
Sbjct: 734  HLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVLIPVIAVLTRRLPP 793

Query: 677  TGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGPY 736
              + +P L KLFR+ W Y  L G                               AV G  
Sbjct: 794  IKEAKPRLQKLFRDFWLYSVLMGF------------------------------AVEGSG 853

Query: 737  MWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELN-ALQNPVSRQGTANEKVVLAQRAAL 796
            +W ++W   V  I+  +P L   + + L   L+ N A++N        NE      R+ +
Sbjct: 854  LWPEEWYEGVCEIATKSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELNE-----LRSTI 913

Query: 797  ATALGGRVDISAM-STISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASR-SAFSCV 856
               L    ++SA+ + +    +TYLL+V  LE  R      +L    S D  R     C 
Sbjct: 914  INLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMR------VLR---STDPDRFQVMFCY 973

Query: 857  FEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKS 916
            FE          ++QC+ A+  + ++  ++ + ++     NE       L  H  FL+ +
Sbjct: 974  FEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEE-----LERHAQFLLVN 1033

Query: 917  MCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLL-----------FSMHNQSPTTIV 976
                   IR VA   L+   DKFP ++W+ + L ++L             +H   P   +
Sbjct: 1034 FNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDI 1093

Query: 977  NDPAWVVTVRSLY---QKVVREWIIR-------SLSYAPCTSQGLLQEMLCKANTWQR-- 1036
             D  + +TV   Y   + +V+++  R       ++ +AP  ++  LQE L K   W    
Sbjct: 1094 PDAPYRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGL 1153

Query: 1037 AQHTPDVASLLSEIRI-GTGKNDCWTGI-QTANIPAVMSAAAAASGGNLNLTKAFNLEVL 1096
            +QHT    +  S +   G  K +   G+ Q    PA +    +    +LNL         
Sbjct: 1154 SQHTGLAMATESILHFAGYNKQNTTLGVTQLTERPACVKKDYSNFMASLNL--------- 1213

Query: 1097 STGIGSATVKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQG 1156
                     +  Y GE+ GM R     G               + +Q LIT      PQ 
Sbjct: 1214 ---------RNRYAGEVHGMIRFSGATGQMSDLNK--------MMVQDLITALDHSHPQ- 1273

Query: 1157 EDESFNAMLLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEG 1216
                        + Q++ K                       TA L+++   D       
Sbjct: 1274 -----------HYTQAMFKL----------------------TAMLISSKDCD------- 1333

Query: 1217 LSQLLRLLCWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGL 1276
              QLL  LCW P  +   + MET +  W WL++    +    + E+  AW  T++ K GL
Sbjct: 1334 -PQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMREMAGAWHMTVEQKFGL 1393

Query: 1277 FSSREAFSGPTAKLRPHLYPGDPE-MPPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQ 1336
            FS+    + P A          P   PPE+ P      H +W+ F   RFE+ +Y S +Q
Sbjct: 1394 FSAETKEADPLAASE----ASQPRPCPPEVTP------HYIWIDFLVQRFEIAKYCSSDQ 1453

Query: 1337 LLLFGRLLQGSMEL-----PWKFSHHPAATGSFFTLMFLGLKFCSCQSQVNLQNFKTGLQ 1396
            + +F  LLQ SM L         + H AA G  F L+ LGL         N     T   
Sbjct: 1454 VEIFSSLLQRSMSLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPN----ATIRN 1513

Query: 1397 LLEDRVYRASLGWFAFEPEWHDINSTNFAQSEAQSVALFVQYLSN--YLKDGSQIDVKGQ 1456
            +L +++Y  +  +F+  P++         +  +  +  +    S+  YL     +    Q
Sbjct: 1514 VLREKIYSTAFDYFSCPPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQ 1573

Query: 1457 VRENGNSVDLNS-CHPVWGQIENY-------TVGRE------------------KRKQLL 1516
               +   + + S      G I  Y       T+ ++                  KR+ LL
Sbjct: 1574 DTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLL 1633

Query: 1517 LMLCQHEADRLEVWAQPVNTKET-------------TSSRPKISSEKWVEYARTAYSVDP 1576
            L L   E +RL  W  P++  E               S    +S ++W +    A+++ P
Sbjct: 1634 LSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAWTISP 1693

Query: 1577 RIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHW 1636
             +A  L +RF     +  EV++ V+     +  +PEA+ + V+   +D ++  L  +  W
Sbjct: 1694 YLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVLCW 1753

Query: 1637 AACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGY 1696
            A       + + +  Y  HP    Y ++VL S+PP+ + F++PQ+VQALRYD    V  Y
Sbjct: 1754 APTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREY 1813

Query: 1697 LLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPV 1756
            +L AA +S + +H  IW+++        +  D +G   +P+ G             LL  
Sbjct: 1814 ILWAAAKSQLLAHQFIWNMK------TNIYLDEEGHQKDPDIGD------------LLEQ 1873

Query: 1757 VRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPK-DERRAGIQRELEKIEMNGDD 1816
            + ++I    +G A D +QREFDFF+K+T++S ++ P PK DER+      L ++++    
Sbjct: 1874 LVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKPYPKGDERKKACLSALSEVKVQ-PG 1933

Query: 1817 LYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNV-----------------------A 1876
             YLP+ P  +V  I   SG P+QSAAK P +  F V                        
Sbjct: 1934 CYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKEGLQCRSDTEDECR 1993

Query: 1877 DRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPER 1936
             ++ D   +  QA IFKVGDDCRQD+LALQ+I L +++F+ VGL+L++FPY V+ T P  
Sbjct: 1994 RQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGC 2053

Query: 1937 GIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLL 1996
            G+IE +P+  SR Q+G  TD G+Y+ F + YG   + +F+ AR NFI S A Y++   LL
Sbjct: 2054 GVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLL 2094

Query: 1997 QPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS 2034
            Q KDRHNGN++ D  G +IHIDFGF+ E+SPGGN+ +E    KL+ EM  ++   G M++
Sbjct: 2114 QIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWE-PDIKLTDEMVMIM--GGKMEA 2094

BLAST of HG10000440 vs. ExPASy TrEMBL
Match: A0A1S3C6N5 (phosphatidylinositol 4-kinase alpha 1 OS=Cucumis melo OX=3656 GN=LOC103497500 PE=4 SV=1)

HSP 1 Score: 3880.5 bits (10062), Expect = 0.0e+00
Identity = 1961/2036 (96.32%), Postives = 1988/2036 (97.64%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEALLEL DLIAQNPSAFFEK++WICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSK 
Sbjct: 1    MEALLELSDLIAQNPSAFFEKLSWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKS 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSIT FYT+FLGYVSKATEITPDFSA
Sbjct: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITSFYTDFLGYVSKATEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGE+VASAISSGGGDSGISR FLMVFSK+FLP+LPSDANKLVDMVIERYLM GS 
Sbjct: 121  EVAVYTGEIVASAISSGGGDSGISRVFLMVFSKNFLPVLPSDANKLVDMVIERYLMTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRN-GSHDSGSRVGDDNISSWKSSATSYGSGILWKSSA 240
            APREQMQGNSDSSAP S+DGS P+N GSHDSGSRVGDDNISSWKSSATSYGSGILWKSS 
Sbjct: 181  APREQMQGNSDSSAPHSVDGSVPQNGGSHDSGSRVGDDNISSWKSSATSYGSGILWKSST 240

Query: 241  DQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWD 300
            DQLGPMFGFNDGGGGGE+LLKQQVS FEEESLESLEKQAIAFE+LAHILDKARIDSWLWD
Sbjct: 241  DQLGPMFGFNDGGGGGEILLKQQVSGFEEESLESLEKQAIAFEMLAHILDKARIDSWLWD 300

Query: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360
            QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG
Sbjct: 301  QVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEG 360

Query: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420
            KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI
Sbjct: 361  KVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLI 420

Query: 421  RLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGR 480
            RLKPLVLTVCAQTDPSDS  N+MFETVSKT+C I+ESCWTKERAPVDTFIMGLTASIRGR
Sbjct: 421  RLKPLVLTVCAQTDPSDSSPNSMFETVSKTMCGIIESCWTKERAPVDTFIMGLTASIRGR 480

Query: 481  NDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540
            NDYEEQ NKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR
Sbjct: 481  NDYEEQENKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLR 540

Query: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLP 600
            LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSA APEATTERLETLP
Sbjct: 541  LQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAAAPEATTERLETLP 600

Query: 601  AGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660
            AGFLLIA GLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF
Sbjct: 601  AGFLLIARGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDF 660

Query: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSG 720
            SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVS AGSVGTTAIQAVSG
Sbjct: 661  SPTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSIAGSVGTTAIQAVSG 720

Query: 721  PYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780
            PYMWDKQWSSAVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA
Sbjct: 721  PYMWDKQWSSAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAA 780

Query: 781  LATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVF 840
            LATALGGRVDISAM TISGVKATYLLAVSFLEITRFSSNGGILNGS SVDASRSAFSCVF
Sbjct: 781  LATALGGRVDISAMGTISGVKATYLLAVSFLEITRFSSNGGILNGSTSVDASRSAFSCVF 840

Query: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSM 900
            EYLKTPNLVPAVLQCLTALVHRAYETAVSWLE+RISEIGNEA +RNSILNAHTCFLIKS+
Sbjct: 841  EYLKTPNLVPAVLQCLTALVHRAYETAVSWLEKRISEIGNEATVRNSILNAHTCFLIKSL 900

Query: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSL 960
            CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSL
Sbjct: 901  CQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSL 960

Query: 961  YQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCW 1020
            YQKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQ TPDVASLLSEIRIGTGKNDCW
Sbjct: 961  YQKVVREWIIRSLSHAPCTSQGLLQEMLCKANTWQRAQQTPDVASLLSEIRIGTGKNDCW 1020

Query: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT 1080
            TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT
Sbjct: 1021 TGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNT 1080

Query: 1081 FGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140
            FGGYQSAG  PTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 FGGYQSAGPAPTGSGIGIGLQRLITGAFPQEPQGEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200
            GGEIDKSQFHETCSQATAFLLANLGTDSK NIEGLSQLLRLLCWCPAYISTSNAMETGVF
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLGTDSKFNIEGLSQLLRLLCWCPAYISTSNAMETGVF 1200

Query: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260
            VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP
Sbjct: 1201 VWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEMP 1260

Query: 1261 PEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATGS 1320
            PEIDPV QIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSM+LPWKFSHHPAATGS
Sbjct: 1261 PEIDPVGQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMQLPWKFSHHPAATGS 1320

Query: 1321 FFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380
            FFTLMFLGLKFCSCQSQVNLQNFK GLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE
Sbjct: 1321 FFTLMFLGLKFCSCQSQVNLQNFKAGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQSE 1380

Query: 1381 AQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQLL 1440
            AQSVALFVQYLSNYLKDGSQ DVKGQVRENGN  D NSCHPVWGQ+ENYTVGREKR+QLL
Sbjct: 1381 AQSVALFVQYLSNYLKDGSQTDVKGQVRENGNLADQNSCHPVWGQMENYTVGREKRRQLL 1440

Query: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN 1500
            LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN
Sbjct: 1441 LMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPAN 1500

Query: 1501 VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560
            VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA
Sbjct: 1501 VTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFLA 1560

Query: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFSH 1620
            PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIF+H
Sbjct: 1561 PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFAH 1620

Query: 1621 ILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQALLPVVRQQIIDGFTGKA 1680
            ILIWH              LQGETCEPETGKEATSTK+NSFQALLPVVRQ IIDGF+GKA
Sbjct: 1621 ILIWH--------------LQGETCEPETGKEATSTKSNSFQALLPVVRQHIIDGFSGKA 1680

Query: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVKGI 1740
            LDVFQREFDFFDKVTSISGVLFPLPKDERRAGI+RELEKIEMNGDDLYLPTAPSKLVKGI
Sbjct: 1681 LDVFQREFDFFDKVTSISGVLFPLPKDERRAGIRRELEKIEMNGDDLYLPTAPSKLVKGI 1740

Query: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALLRD 1800
            QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVI+LLRD
Sbjct: 1741 QVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVISLLRD 1800

Query: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860
            VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP
Sbjct: 1801 VFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVGSP 1860

Query: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNMRF 1920
            SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNMRF
Sbjct: 1861 SFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNMRF 1920

Query: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSGLP 1980
            ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGI+TTVALMLDSGLP
Sbjct: 1921 ESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGIITTVALMLDSGLP 1980

Query: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 CFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2022

BLAST of HG10000440 vs. ExPASy TrEMBL
Match: A0A6J1G768 (phosphatidylinositol 4-kinase alpha 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451444 PE=4 SV=1)

HSP 1 Score: 3691.7 bits (9572), Expect = 0.0e+00
Identity = 1859/2038 (91.22%), Postives = 1940/2038 (95.19%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCDLIA+NP+AF+EK++WICSRCPPPDALL++SPRVSRSQLNA+LAIARFLSKC
Sbjct: 1    MEALFELCDLIAENPTAFYEKLSWICSRCPPPDALLAESPRVSRSQLNAILAIARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            AD  E RPKAVVLEFIRSIPSSF+ SFWP TYGNDSITLFYTEFL YVS+A EITPDFSA
Sbjct: 61   ADCFELRPKAVVLEFIRSIPSSFHPSFWPQTYGNDSITLFYTEFLRYVSRAAEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISS  GDS ISRSFLMV SKHF P+LPSDANK+V+MVIER+L+ GS 
Sbjct: 121  EVAVYTGEVVASAISSSAGDSSISRSFLMVLSKHFFPVLPSDANKIVEMVIERFLLTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRNGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSAD 240
            APRE MQGNSDSSA  SI+GSSP+NG        G+DNISSWKSSA+SYGSG+LWKSSAD
Sbjct: 181  APREHMQGNSDSSAFHSIEGSSPQNG--------GEDNISSWKSSASSYGSGVLWKSSAD 240

Query: 241  QLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWDQ 300
            QLG MFGFNDGGGGGEVLLKQQV+ FEEESLE +EKQAIAFELLAHILDKARIDS LWDQ
Sbjct: 241  QLGSMFGFNDGGGGGEVLLKQQVAIFEEESLECVEKQAIAFELLAHILDKARIDSSLWDQ 300

Query: 301  VRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEGK 360
             RSIAKKQLQSLLIFVKIRKRDWNEQGS+LKARINAKLLVYQAAAKLKLKSLGSLD+EGK
Sbjct: 301  ARSIAKKQLQSLLIFVKIRKRDWNEQGSVLKARINAKLLVYQAAAKLKLKSLGSLDNEGK 360

Query: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLIR 420
            VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLG+IAVAWKGQPLRVLLIR
Sbjct: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGQIAVAWKGQPLRVLLIR 420

Query: 421  LKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRN 480
            LKPLVLTVCAQTDP+DS  N MFETVSKT+CEIVESCWTKERAPVDTFIMGLTASIRGRN
Sbjct: 421  LKPLVLTVCAQTDPTDSSQNTMFETVSKTMCEIVESCWTKERAPVDTFIMGLTASIRGRN 480

Query: 481  DYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRL 540
            DYEEQVNKE+ VPPLQLNGIRLLANLNVAVKK EIVDMILPLFIESLEEGDAS+PC+LRL
Sbjct: 481  DYEEQVNKEREVPPLQLNGIRLLANLNVAVKKPEIVDMILPLFIESLEEGDASTPCVLRL 540

Query: 541  QFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLPA 600
            QFLDA+SRMASLGFEKSYRETVVLMTRSYLSKLSNAAS PPE     PEATTERLETLPA
Sbjct: 541  QFLDAVSRMASLGFEKSYRETVVLMTRSYLSKLSNAASAPPESGGGTPEATTERLETLPA 600

Query: 601  GFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDFS 660
            GF LIASGLA+ KLRSDYRYRLLSLCSDVGLAADSRN+RSGADFLGPLLPAVAEICSDFS
Sbjct: 601  GFFLIASGLANPKLRSDYRYRLLSLCSDVGLAADSRNSRSGADFLGPLLPAVAEICSDFS 660

Query: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGP 720
            PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNS++PSKAISTTVS+AGS+GTTAIQAVSGP
Sbjct: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSALPSKAISTTVSTAGSIGTTAIQAVSGP 720

Query: 721  YMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAAL 780
            YMWDKQWS+AVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTAN+KVVLAQRAAL
Sbjct: 721  YMWDKQWSAAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANDKVVLAQRAAL 780

Query: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVFE 840
            ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSA SCVFE
Sbjct: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAISCVFE 840

Query: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSMC 900
            YLKTPNLVPAVLQCLTALVHRAYETAVSWLE RIS+IGNEAA+RNSILNAHTCFLIKSMC
Sbjct: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEHRISDIGNEAAVRNSILNAHTCFLIKSMC 900

Query: 901  QREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSLY 960
            QREDHIREVAVTLLTQFRD+FPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSLY
Sbjct: 901  QREDHIREVAVTLLTQFRDRFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSLY 960

Query: 961  QKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCWT 1020
            QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDV SLLSEIRIGTGKN+CWT
Sbjct: 961  QKVVREWIIRSLSFAPCTSQGLLQEMLCKANTWQRAQHTPDVVSLLSEIRIGTGKNECWT 1020

Query: 1021 GIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080
            G+QTANIPAVM+AAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF
Sbjct: 1021 GVQTANIPAVMAAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080

Query: 1081 GGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQ-GEDESFNAMLLLKFVQSLQKFVNNAET 1140
            GGYQS G  P GSGIGIGLQRLITGAFPQEPQ  EDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 GGYQSTGTAPAGSGIGIGLQRLITGAFPQEPQAAEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANL-GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            GGEIDKSQFHETCSQATAFLLANL   DSKSNIEGLSQLLRLLCWCPAYIS SNAMETGV
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLDNADSKSNIEGLSQLLRLLCWCPAYISRSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGC+VLAELVDAWLWTIDTKRGLFSS+EA SGPTAKLRPHLYPGDPE+
Sbjct: 1201 FVWTWLVSAAPQLGCIVLAELVDAWLWTIDTKRGLFSSKEALSGPTAKLRPHLYPGDPEL 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPE DPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEFDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQ+QVNL+NF+TGL+LLEDRVYRASLGWF+FEPEWHDINS+NFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQTQVNLRNFETGLRLLEDRVYRASLGWFSFEPEWHDINSSNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALF QYLSN LKDGSQIDVKGQVRENGNS D + CHPVWG ++NY VGREKR+QL
Sbjct: 1381 EAQSVALFAQYLSNSLKDGSQIDVKGQVRENGNSADQSFCHPVWGPMDNYAVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWA PVNTKETTS RPKISSEKW+EYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWANPVNTKETTSLRPKISSEKWIEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NV LR+EVS QVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSI QAIEF+
Sbjct: 1501 NVALRSEVSHQVQSHILDIRSIPEALPYFVSPKAVDENSVLLQQLPHWAACSIVQAIEFI 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            AP+YKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR+DDGKLVEGYLLRAAQRSDIFS
Sbjct: 1561 APSYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRHDDGKLVEGYLLRAAQRSDIFS 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPET-GKEATSTKNNSFQALLPVVRQQIIDGFTG 1680
            HILIWH              LQGETCEPET GK+AT  K ++FQALLPVVRQ+IIDGF+ 
Sbjct: 1621 HILIWH--------------LQGETCEPETAGKDATVGKMSAFQALLPVVRQKIIDGFSP 1680

Query: 1681 KALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVK 1740
             A D+FQREFDFFDKVTSISGVLFP+PKDERRAGI++ELEKIEM+GDDLYLPTAP KLV+
Sbjct: 1681 SARDIFQREFDFFDKVTSISGVLFPIPKDERRAGIRKELEKIEMDGDDLYLPTAPGKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNV DRDGD ND+KPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVIDRDGDHNDIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDVFEAVGL+LYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDVFEAVGLSLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNM 1920
            SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYL+ARRYMDGILTTVALMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLSARRYMDGILTTVALMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2016

BLAST of HG10000440 vs. ExPASy TrEMBL
Match: A0A6J1L2P9 (phosphatidylinositol 4-kinase alpha 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499868 PE=4 SV=1)

HSP 1 Score: 3686.7 bits (9559), Expect = 0.0e+00
Identity = 1856/2038 (91.07%), Postives = 1939/2038 (95.14%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCDLIA+NP+AF+EK++WICSRCPPPDALL++SPRVSRSQLNA+LAIARFLSKC
Sbjct: 1    MEALFELCDLIAENPTAFYEKLSWICSRCPPPDALLAESPRVSRSQLNAILAIARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            AD  E RPKAVVLEFIRSI SSF+ SFWP TYGNDSITLFYTEFL YVS+A EITPDFSA
Sbjct: 61   ADCFEIRPKAVVLEFIRSISSSFHPSFWPQTYGNDSITLFYTEFLRYVSRAAEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISS  GDS ISRSFLMV SKHFLP+LPSDANK+VDMVIER+L+ GS 
Sbjct: 121  EVAVYTGEVVASAISSSSGDSSISRSFLMVLSKHFLPVLPSDANKIVDMVIERFLLTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRNGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSAD 240
            APRE MQGNSDSSA  SI+GSSP NG        G+DNISSWKSSATSYGSG+LWKSSAD
Sbjct: 181  APREHMQGNSDSSAFHSIEGSSPGNG--------GEDNISSWKSSATSYGSGVLWKSSAD 240

Query: 241  QLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWDQ 300
            QLG MFGFNDGGGGGEVLLKQQV+ FEEESLE +EKQAIAFELLAHILDKARIDS LWDQ
Sbjct: 241  QLGSMFGFNDGGGGGEVLLKQQVAIFEEESLECVEKQAIAFELLAHILDKARIDSSLWDQ 300

Query: 301  VRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEGK 360
             RSIAKKQLQSLLIFVKIRKRDWNEQGS+LKARINAKLLVYQAAAKLKLKSLGSLD+EGK
Sbjct: 301  ARSIAKKQLQSLLIFVKIRKRDWNEQGSVLKARINAKLLVYQAAAKLKLKSLGSLDNEGK 360

Query: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLIR 420
            VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLG+IAVAWKGQPLRVLLIR
Sbjct: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGQIAVAWKGQPLRVLLIR 420

Query: 421  LKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRN 480
            LKPLVLTVCAQTDP+++  N MFETVSKT+CEIVESCWTKERAPVDTFIMGLTASIRGRN
Sbjct: 421  LKPLVLTVCAQTDPTENSQNTMFETVSKTMCEIVESCWTKERAPVDTFIMGLTASIRGRN 480

Query: 481  DYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRL 540
            DYEEQVNKE+ VPPLQLNGIRLLANLNVAVKK EIVDMILPLFIESLEEGDAS+PC+LRL
Sbjct: 481  DYEEQVNKEREVPPLQLNGIRLLANLNVAVKKPEIVDMILPLFIESLEEGDASTPCVLRL 540

Query: 541  QFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLPA 600
            QFLDA+SRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE     PEATTERLETLPA
Sbjct: 541  QFLDAVSRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEDGGGTPEATTERLETLPA 600

Query: 601  GFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDFS 660
            GF LIASGLA+ KLRSDYRYRLLSLCSDVGLAADSRN+RSGADFLGPLLPAVAEICSDFS
Sbjct: 601  GFFLIASGLANPKLRSDYRYRLLSLCSDVGLAADSRNSRSGADFLGPLLPAVAEICSDFS 660

Query: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGP 720
            PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNS++PSKAISTTVS+AGSVGTTAIQAVSGP
Sbjct: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSALPSKAISTTVSTAGSVGTTAIQAVSGP 720

Query: 721  YMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAAL 780
            YMWDKQWS+AVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTAN+KVVLAQRAAL
Sbjct: 721  YMWDKQWSAAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANDKVVLAQRAAL 780

Query: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVFE 840
            ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGS+SVDASRSA SCVFE
Sbjct: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSLSVDASRSAISCVFE 840

Query: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSMC 900
            YLKTPNLVPAVLQCLTALVHRAYETAVSWLE RIS+IGNEAA+RNSILNAHTCFLIKSMC
Sbjct: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEHRISDIGNEAAVRNSILNAHTCFLIKSMC 900

Query: 901  QREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSLY 960
            QREDHIREVAVTLLTQFRD+FPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSLY
Sbjct: 901  QREDHIREVAVTLLTQFRDRFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSLY 960

Query: 961  QKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCWT 1020
            QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDV SLLSEIRIGTGKN+CWT
Sbjct: 961  QKVVREWIIRSLSFAPCTSQGLLQEMLCKANTWQRAQHTPDVVSLLSEIRIGTGKNECWT 1020

Query: 1021 GIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080
            G+QTANIPAVM+AAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF
Sbjct: 1021 GVQTANIPAVMAAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080

Query: 1081 GGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQ-GEDESFNAMLLLKFVQSLQKFVNNAET 1140
            GGYQS G  P GSG+GIGLQRLITGAFPQEPQ  EDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 GGYQSTGTAPAGSGLGIGLQRLITGAFPQEPQAAEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANL-GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            GGEIDKSQFHETCSQATAFLLANL   DSKSNIEGLSQLLRLLCWCPAYIS SNAMETGV
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLDNADSKSNIEGLSQLLRLLCWCPAYISRSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGC+VLAELVDAWLWTIDTKRGLFSS+EA SGPTAKLRPHLYPGDPE+
Sbjct: 1201 FVWTWLVSAAPQLGCIVLAELVDAWLWTIDTKRGLFSSKEALSGPTAKLRPHLYPGDPEL 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPE DPVEQII+HRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEFDPVEQIISHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQ+QVNL+NF+TGL+LLEDRVYR SLGWF+FEPEWHDINS+NFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQTQVNLRNFETGLRLLEDRVYRTSLGWFSFEPEWHDINSSNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALF QYLSN LKDGSQIDVKGQVRENGNS D + CHPVWG ++NY VGREKR+QL
Sbjct: 1381 EAQSVALFAQYLSNSLKDGSQIDVKGQVRENGNSADQSFCHPVWGPMDNYAVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWA PVNTKETTS RPKIS+EKWVEYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWANPVNTKETTSLRPKISTEKWVEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NV LR+EVS QVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSI QAIEF+
Sbjct: 1501 NVALRSEVSHQVQSHILDIRSIPEALPYFVSPKAVDENSVLLQQLPHWAACSIVQAIEFI 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            AP+YKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR+DDGKLVEGYLLRAAQRSDIFS
Sbjct: 1561 APSYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRHDDGKLVEGYLLRAAQRSDIFS 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPET-GKEATSTKNNSFQALLPVVRQQIIDGFTG 1680
            HILIWH              LQGETCEPET GK+AT  K ++FQALLPVVRQ+IIDGF+ 
Sbjct: 1621 HILIWH--------------LQGETCEPETAGKDATVGKMSAFQALLPVVRQKIIDGFSP 1680

Query: 1681 KALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVK 1740
             A D+FQREFDFFDKVTSISGVLFP+PKDERRAGI++ELEKIEM+GDDLYLPTAP KLV+
Sbjct: 1681 SARDIFQREFDFFDKVTSISGVLFPIPKDERRAGIRKELEKIEMDGDDLYLPTAPGKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNV DRDGD ND+KPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVIDRDGDHNDIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDVFEAVG++LYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDVFEAVGISLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNM 1920
            SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYL+ARRYMDGILTTVALMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLSARRYMDGILTTVALMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2016

BLAST of HG10000440 vs. ExPASy TrEMBL
Match: A0A6J1G7F1 (phosphatidylinositol 4-kinase alpha 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451444 PE=4 SV=1)

HSP 1 Score: 3685.2 bits (9555), Expect = 0.0e+00
Identity = 1858/2038 (91.17%), Postives = 1939/2038 (95.14%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCDLIA+NP+AF+EK++WICSRCPPPDALL++SPRVSRSQLNA+LAIARFLSKC
Sbjct: 1    MEALFELCDLIAENPTAFYEKLSWICSRCPPPDALLAESPRVSRSQLNAILAIARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            AD  E RPKAVVLEFIRSIPSSF+ SFWP TYGNDSITLFYTEFL YVS+A EITPDFSA
Sbjct: 61   ADCFELRPKAVVLEFIRSIPSSFHPSFWPQTYGNDSITLFYTEFLRYVSRAAEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISS  GDS ISRSFLMV SKHF P+LPSDANK+V+MVIER+L+ GS 
Sbjct: 121  EVAVYTGEVVASAISSSAGDSSISRSFLMVLSKHFFPVLPSDANKIVEMVIERFLLTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRNGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSAD 240
            APRE MQGNSDSSA  SI+GSSP+NG        G+DNISSWKSSA+SYGSG+LWKSSAD
Sbjct: 181  APREHMQGNSDSSAFHSIEGSSPQNG--------GEDNISSWKSSASSYGSGVLWKSSAD 240

Query: 241  QLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWDQ 300
            QLG MFGFNDGGGGGEVLLKQQV+ FEEESLE +EKQAIAFELLAHILDKARIDS LWDQ
Sbjct: 241  QLGSMFGFNDGGGGGEVLLKQQVAIFEEESLECVEKQAIAFELLAHILDKARIDSSLWDQ 300

Query: 301  VRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEGK 360
             RSIAKKQLQSLLIFVKIRKRDWNEQGS+LKARINAKLLVYQAAAKLKLKSLGSLD+EGK
Sbjct: 301  ARSIAKKQLQSLLIFVKIRKRDWNEQGSVLKARINAKLLVYQAAAKLKLKSLGSLDNEGK 360

Query: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLIR 420
            VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLG+IAVAWKGQPLRVLLIR
Sbjct: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGQIAVAWKGQPLRVLLIR 420

Query: 421  LKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRN 480
            LKPLVLTVCAQTDP+DS  N MFETVSKT+CEIVESCWTKERAPVDTFIMGLTASIRGRN
Sbjct: 421  LKPLVLTVCAQTDPTDSSQNTMFETVSKTMCEIVESCWTKERAPVDTFIMGLTASIRGRN 480

Query: 481  DYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRL 540
            DYEEQVNKE+ VPPLQLNGIRLLANLNVAVKK EIVDMILPLFIESLEEGDAS+PC+LRL
Sbjct: 481  DYEEQVNKEREVPPLQLNGIRLLANLNVAVKKPEIVDMILPLFIESLEEGDASTPCVLRL 540

Query: 541  QFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLPA 600
            QFLDA+SRMASLGFEKSYRETVVLMTRSYLSKLSNAAS PPE     PEATTERLETLPA
Sbjct: 541  QFLDAVSRMASLGFEKSYRETVVLMTRSYLSKLSNAASAPPESGGGTPEATTERLETLPA 600

Query: 601  GFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDFS 660
            GF LIASGLA+ KLRSDYRYRLLSLCSDVGLAADSRN+ SGADFLGPLLPAVAEICSDFS
Sbjct: 601  GFFLIASGLANPKLRSDYRYRLLSLCSDVGLAADSRNS-SGADFLGPLLPAVAEICSDFS 660

Query: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGP 720
            PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNS++PSKAISTTVS+AGS+GTTAIQAVSGP
Sbjct: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSALPSKAISTTVSTAGSIGTTAIQAVSGP 720

Query: 721  YMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAAL 780
            YMWDKQWS+AVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTAN+KVVLAQRAAL
Sbjct: 721  YMWDKQWSAAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANDKVVLAQRAAL 780

Query: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVFE 840
            ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSA SCVFE
Sbjct: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAISCVFE 840

Query: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSMC 900
            YLKTPNLVPAVLQCLTALVHRAYETAVSWLE RIS+IGNEAA+RNSILNAHTCFLIKSMC
Sbjct: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEHRISDIGNEAAVRNSILNAHTCFLIKSMC 900

Query: 901  QREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSLY 960
            QREDHIREVAVTLLTQFRD+FPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSLY
Sbjct: 901  QREDHIREVAVTLLTQFRDRFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSLY 960

Query: 961  QKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCWT 1020
            QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDV SLLSEIRIGTGKN+CWT
Sbjct: 961  QKVVREWIIRSLSFAPCTSQGLLQEMLCKANTWQRAQHTPDVVSLLSEIRIGTGKNECWT 1020

Query: 1021 GIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080
            G+QTANIPAVM+AAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF
Sbjct: 1021 GVQTANIPAVMAAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080

Query: 1081 GGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQ-GEDESFNAMLLLKFVQSLQKFVNNAET 1140
            GGYQS G  P GSGIGIGLQRLITGAFPQEPQ  EDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 GGYQSTGTAPAGSGIGIGLQRLITGAFPQEPQAAEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANL-GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            GGEIDKSQFHETCSQATAFLLANL   DSKSNIEGLSQLLRLLCWCPAYIS SNAMETGV
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLDNADSKSNIEGLSQLLRLLCWCPAYISRSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGC+VLAELVDAWLWTIDTKRGLFSS+EA SGPTAKLRPHLYPGDPE+
Sbjct: 1201 FVWTWLVSAAPQLGCIVLAELVDAWLWTIDTKRGLFSSKEALSGPTAKLRPHLYPGDPEL 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPE DPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEFDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQ+QVNL+NF+TGL+LLEDRVYRASLGWF+FEPEWHDINS+NFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQTQVNLRNFETGLRLLEDRVYRASLGWFSFEPEWHDINSSNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALF QYLSN LKDGSQIDVKGQVRENGNS D + CHPVWG ++NY VGREKR+QL
Sbjct: 1381 EAQSVALFAQYLSNSLKDGSQIDVKGQVRENGNSADQSFCHPVWGPMDNYAVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWA PVNTKETTS RPKISSEKW+EYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWANPVNTKETTSLRPKISSEKWIEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NV LR+EVS QVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSI QAIEF+
Sbjct: 1501 NVALRSEVSHQVQSHILDIRSIPEALPYFVSPKAVDENSVLLQQLPHWAACSIVQAIEFI 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            AP+YKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR+DDGKLVEGYLLRAAQRSDIFS
Sbjct: 1561 APSYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRHDDGKLVEGYLLRAAQRSDIFS 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPET-GKEATSTKNNSFQALLPVVRQQIIDGFTG 1680
            HILIWH              LQGETCEPET GK+AT  K ++FQALLPVVRQ+IIDGF+ 
Sbjct: 1621 HILIWH--------------LQGETCEPETAGKDATVGKMSAFQALLPVVRQKIIDGFSP 1680

Query: 1681 KALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVK 1740
             A D+FQREFDFFDKVTSISGVLFP+PKDERRAGI++ELEKIEM+GDDLYLPTAP KLV+
Sbjct: 1681 SARDIFQREFDFFDKVTSISGVLFPIPKDERRAGIRKELEKIEMDGDDLYLPTAPGKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNV DRDGD ND+KPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVIDRDGDHNDIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDVFEAVGL+LYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDVFEAVGLSLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNM 1920
            SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYL+ARRYMDGILTTVALMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLSARRYMDGILTTVALMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2015

BLAST of HG10000440 vs. ExPASy TrEMBL
Match: A0A6J1L099 (phosphatidylinositol 4-kinase alpha 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499868 PE=4 SV=1)

HSP 1 Score: 3680.2 bits (9542), Expect = 0.0e+00
Identity = 1855/2038 (91.02%), Postives = 1938/2038 (95.09%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCDLIA+NP+AF+EK++WICSRCPPPDALL++SPRVSRSQLNA+LAIARFLSKC
Sbjct: 1    MEALFELCDLIAENPTAFYEKLSWICSRCPPPDALLAESPRVSRSQLNAILAIARFLSKC 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
            AD  E RPKAVVLEFIRSI SSF+ SFWP TYGNDSITLFYTEFL YVS+A EITPDFSA
Sbjct: 61   ADCFEIRPKAVVLEFIRSISSSFHPSFWPQTYGNDSITLFYTEFLRYVSRAAEITPDFSA 120

Query: 121  EVAVYTGEVVASAISSGGGDSGISRSFLMVFSKHFLPILPSDANKLVDMVIERYLMMGSG 180
            EVAVYTGEVVASAISS  GDS ISRSFLMV SKHFLP+LPSDANK+VDMVIER+L+ GS 
Sbjct: 121  EVAVYTGEVVASAISSSSGDSSISRSFLMVLSKHFLPVLPSDANKIVDMVIERFLLTGSC 180

Query: 181  APREQMQGNSDSSAPQSIDGSSPRNGSHDSGSRVGDDNISSWKSSATSYGSGILWKSSAD 240
            APRE MQGNSDSSA  SI+GSSP NG        G+DNISSWKSSATSYGSG+LWKSSAD
Sbjct: 181  APREHMQGNSDSSAFHSIEGSSPGNG--------GEDNISSWKSSATSYGSGVLWKSSAD 240

Query: 241  QLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFELLAHILDKARIDSWLWDQ 300
            QLG MFGFNDGGGGGEVLLKQQV+ FEEESLE +EKQAIAFELLAHILDKARIDS LWDQ
Sbjct: 241  QLGSMFGFNDGGGGGEVLLKQQVAIFEEESLECVEKQAIAFELLAHILDKARIDSSLWDQ 300

Query: 301  VRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQAAAKLKLKSLGSLDSEGK 360
             RSIAKKQLQSLLIFVKIRKRDWNEQGS+LKARINAKLLVYQAAAKLKLKSLGSLD+EGK
Sbjct: 301  ARSIAKKQLQSLLIFVKIRKRDWNEQGSVLKARINAKLLVYQAAAKLKLKSLGSLDNEGK 360

Query: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGKIAVAWKGQPLRVLLIR 420
            VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLG+IAVAWKGQPLRVLLIR
Sbjct: 361  VAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVLGQIAVAWKGQPLRVLLIR 420

Query: 421  LKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKERAPVDTFIMGLTASIRGRN 480
            LKPLVLTVCAQTDP+++  N MFETVSKT+CEIVESCWTKERAPVDTFIMGLTASIRGRN
Sbjct: 421  LKPLVLTVCAQTDPTENSQNTMFETVSKTMCEIVESCWTKERAPVDTFIMGLTASIRGRN 480

Query: 481  DYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPLFIESLEEGDASSPCLLRL 540
            DYEEQVNKE+ VPPLQLNGIRLLANLNVAVKK EIVDMILPLFIESLEEGDAS+PC+LRL
Sbjct: 481  DYEEQVNKEREVPPLQLNGIRLLANLNVAVKKPEIVDMILPLFIESLEEGDASTPCVLRL 540

Query: 541  QFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEGSAPAPEATTERLETLPA 600
            QFLDA+SRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE     PEATTERLETLPA
Sbjct: 541  QFLDAVSRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPEDGGGTPEATTERLETLPA 600

Query: 601  GFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGADFLGPLLPAVAEICSDFS 660
            GF LIASGLA+ KLRSDYRYRLLSLCSDVGLAADSRN+ SGADFLGPLLPAVAEICSDFS
Sbjct: 601  GFFLIASGLANPKLRSDYRYRLLSLCSDVGLAADSRNS-SGADFLGPLLPAVAEICSDFS 660

Query: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSSVPSKAISTTVSSAGSVGTTAIQAVSGP 720
            PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNS++PSKAISTTVS+AGSVGTTAIQAVSGP
Sbjct: 661  PTGDVEPSLLKLFRNLWFYVALFGLAPPIQNSALPSKAISTTVSTAGSVGTTAIQAVSGP 720

Query: 721  YMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANEKVVLAQRAAL 780
            YMWDKQWS+AVQ ISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTAN+KVVLAQRAAL
Sbjct: 721  YMWDKQWSAAVQLISQGTPPLVVSTVKWLEDELELNALQNPVSRQGTANDKVVLAQRAAL 780

Query: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSISVDASRSAFSCVFE 840
            ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGS+SVDASRSA SCVFE
Sbjct: 781  ATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGILNGSLSVDASRSAISCVFE 840

Query: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEAAIRNSILNAHTCFLIKSMC 900
            YLKTPNLVPAVLQCLTALVHRAYETAVSWLE RIS+IGNEAA+RNSILNAHTCFLIKSMC
Sbjct: 841  YLKTPNLVPAVLQCLTALVHRAYETAVSWLEHRISDIGNEAAVRNSILNAHTCFLIKSMC 900

Query: 901  QREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQSPTTIVNDPAWVVTVRSLY 960
            QREDHIREVAVTLLTQFRD+FPQVMWNSSCLDSLLFSMHN SPTTIVNDPAWVVTVRSLY
Sbjct: 901  QREDHIREVAVTLLTQFRDRFPQVMWNSSCLDSLLFSMHNHSPTTIVNDPAWVVTVRSLY 960

Query: 961  QKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPDVASLLSEIRIGTGKNDCWT 1020
            QKVVREWIIRSLS+APCTSQGLLQEMLCKANTWQRAQHTPDV SLLSEIRIGTGKN+CWT
Sbjct: 961  QKVVREWIIRSLSFAPCTSQGLLQEMLCKANTWQRAQHTPDVVSLLSEIRIGTGKNECWT 1020

Query: 1021 GIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080
            G+QTANIPAVM+AAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF
Sbjct: 1021 GVQTANIPAVMAAAAAASGGNLNLTKAFNLEVLSTGIGSATVKCNYVGEIAGMRRLYNTF 1080

Query: 1081 GGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQ-GEDESFNAMLLLKFVQSLQKFVNNAET 1140
            GGYQS G  P GSG+GIGLQRLITGAFPQEPQ  EDESFNAMLLLKFVQSLQKFVNNAET
Sbjct: 1081 GGYQSTGTAPAGSGLGIGLQRLITGAFPQEPQAAEDESFNAMLLLKFVQSLQKFVNNAET 1140

Query: 1141 GGEIDKSQFHETCSQATAFLLANL-GTDSKSNIEGLSQLLRLLCWCPAYISTSNAMETGV 1200
            GGEIDKSQFHETCSQATAFLLANL   DSKSNIEGLSQLLRLLCWCPAYIS SNAMETGV
Sbjct: 1141 GGEIDKSQFHETCSQATAFLLANLDNADSKSNIEGLSQLLRLLCWCPAYISRSNAMETGV 1200

Query: 1201 FVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFSGPTAKLRPHLYPGDPEM 1260
            FVWTWLVSAAPQLGC+VLAELVDAWLWTIDTKRGLFSS+EA SGPTAKLRPHLYPGDPE+
Sbjct: 1201 FVWTWLVSAAPQLGCIVLAELVDAWLWTIDTKRGLFSSKEALSGPTAKLRPHLYPGDPEL 1260

Query: 1261 PPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSMELPWKFSHHPAATG 1320
            PPE DPVEQII+HRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGS+ELPWKFSHHPAATG
Sbjct: 1261 PPEFDPVEQIISHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQGSIELPWKFSHHPAATG 1320

Query: 1321 SFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWFAFEPEWHDINSTNFAQS 1380
            SFFTLMFLGLKFCSCQ+QVNL+NF+TGL+LLEDRVYR SLGWF+FEPEWHDINS+NFAQS
Sbjct: 1321 SFFTLMFLGLKFCSCQTQVNLRNFETGLRLLEDRVYRTSLGWFSFEPEWHDINSSNFAQS 1380

Query: 1381 EAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDLNSCHPVWGQIENYTVGREKRKQL 1440
            EAQSVALF QYLSN LKDGSQIDVKGQVRENGNS D + CHPVWG ++NY VGREKR+QL
Sbjct: 1381 EAQSVALFAQYLSNSLKDGSQIDVKGQVRENGNSADQSFCHPVWGPMDNYAVGREKRRQL 1440

Query: 1441 LLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYARTAYSVDPRIAFSLVSRFPA 1500
            LLMLCQHEADRLEVWA PVNTKETTS RPKIS+EKWVEYARTAYSVDPRIAFSLVSRFPA
Sbjct: 1441 LLMLCQHEADRLEVWANPVNTKETTSLRPKISTEKWVEYARTAYSVDPRIAFSLVSRFPA 1500

Query: 1501 NVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLLQQLPHWAACSITQAIEFL 1560
            NV LR+EVS QVQSHILDIR IPEALPYFVSPKAVDENSVLLQQLPHWAACSI QAIEF+
Sbjct: 1501 NVALRSEVSHQVQSHILDIRSIPEALPYFVSPKAVDENSVLLQQLPHWAACSIVQAIEFI 1560

Query: 1561 APAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDGKLVEGYLLRAAQRSDIFS 1620
            AP+YKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALR+DDGKLVEGYLLRAAQRSDIFS
Sbjct: 1561 APSYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRHDDGKLVEGYLLRAAQRSDIFS 1620

Query: 1621 HILIWHLQVFIVYSNRLSFDLQGETCEPET-GKEATSTKNNSFQALLPVVRQQIIDGFTG 1680
            HILIWH              LQGETCEPET GK+AT  K ++FQALLPVVRQ+IIDGF+ 
Sbjct: 1621 HILIWH--------------LQGETCEPETAGKDATVGKMSAFQALLPVVRQKIIDGFSP 1680

Query: 1681 KALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEMNGDDLYLPTAPSKLVK 1740
             A D+FQREFDFFDKVTSISGVLFP+PKDERRAGI++ELEKIEM+GDDLYLPTAP KLV+
Sbjct: 1681 SARDIFQREFDFFDKVTSISGVLFPIPKDERRAGIRKELEKIEMDGDDLYLPTAPGKLVR 1740

Query: 1741 GIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVGDDCRQDVLALQVIALL 1800
            GIQVDSGIPLQSAAKVPIMITFNV DRDGD ND+KPQACIFKVGDDCRQDVLALQVI+LL
Sbjct: 1741 GIQVDSGIPLQSAAKVPIMITFNVIDRDGDHNDIKPQACIFKVGDDCRQDVLALQVISLL 1800

Query: 1801 RDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860
            RDVFEAVG++LYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG
Sbjct: 1801 RDVFEAVGISLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETTDGGLYEIFQQDYGPVG 1860

Query: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLIHIDFGFILETSPGGNM 1920
            SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFD+LGRLIHIDFGFILETSPGGNM
Sbjct: 1861 SPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDNLGRLIHIDFGFILETSPGGNM 1920

Query: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARRYMDGILTTVALMLDSG 1980
            RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYL+ARRYMDGILTTVALMLDSG
Sbjct: 1921 RFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLSARRYMDGILTTVALMLDSG 1980

Query: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2036
            LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK
Sbjct: 1981 LPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTAGYDLIQYLQQGIEK 2015

BLAST of HG10000440 vs. TAIR 10
Match: AT1G49340.2 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2902.5 bits (7523), Expect = 0.0e+00
Identity = 1465/2055 (71.29%), Postives = 1714/2055 (83.41%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCD+IA+NP  F EK+AWIC RCP  + LL++SPRVSRS LNAVLA+AR +SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
             +S++ R K+VV EF+ +IP+SF  SFWP ++ +  I+ FY +FL Y+S A +++P+F  
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVAVYTGEVVASAISSGGGDS----GISRSFLMVFSKHFLPILPSDANKLVDMVIERYLM 180
            EVA +TGEVV +AI+   GDS     IS++FL+  S+HF  IL SD +KL+ M+++++++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  -MGSGAPREQMQGNSDSSA--PQSIDGSSPRNGSHDSG----SRVGDD------NISSWK 240
                 +P+EQ Q NS +S     S  GS      + SG    +  GD+      N+SS  
Sbjct: 181  NRAPASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSSKS 240

Query: 241  SSA-TSYGSGILWKSSADQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFE 300
            SS+    G  I+WKS  DQL   FGF++G GG   + +QQV+SFE+ES+ESLEKQ IAF 
Sbjct: 241  SSSVVMNGGSIVWKSGVDQLS--FGFSEGSGGANPVFRQQVASFEDESIESLEKQEIAFR 300

Query: 301  LLAHILDKARIDSWLWDQVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQ 360
            L+ HILDK +IDS L DQVR IAK+QLQS+  F+K RKRDWNEQG +LK R+NAKL VYQ
Sbjct: 301  LITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLKTRVNAKLSVYQ 360

Query: 361  AAAKLKLKSLGSLDSEGKVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVL 420
            AAAK+K+KSL SL+++GK +K+LV ET ALL+DAA+AC+ S+WRK++ CEELF  LL+ +
Sbjct: 361  AAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKACEELFDSLLSGI 420

Query: 421  GKIAVAWKGQPLRVLLIRLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKER 480
             KIAVA  GQPLRVLLIRLKPLVL VCA  D       AM E++ KT C I+ES W K+R
Sbjct: 421  AKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSCVIIESAWAKDR 480

Query: 481  APVDTFIMGLTASIRGRNDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPL 540
            APVD FIMGL +SIR RNDYEEQV++EK VP +QLN IRLLA+LNVAVKK E+ DMILPL
Sbjct: 481  APVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVKKPEVADMILPL 540

Query: 541  FIESLEEGDASSPCLLRLQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE 600
            FIESLEEGDAS+P  LRLQ LDA+SR+A+LGF+KSYRETVVLMTRSYLSKLS+  S   E
Sbjct: 541  FIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLSKLSSVGS--VE 600

Query: 601  GSAPAPEATTERLETLPAGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGA 660
                APEATTER+ETLPAGFL IASGL  +KLRSDYR+RLLSLCSDVGLAA+S++  SG 
Sbjct: 601  SKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAAESKSGGSGV 660

Query: 661  DFLGPLLPAVAEICSDFSPTGDVEPSLLKLFRNLWFYVALFGLAPPI-QNSSVPSKAIST 720
            DFLGPLLPAVAEICSDF PT DVEPSLLKLFRNLWFY+ALFGLAPPI +  + P K+ S 
Sbjct: 661  DFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTPTPPLKSTSN 720

Query: 721  TVSSAGSVGTTAIQAVSGPYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNP 780
            +V+S GS+  TA+QAV GPYMWD QW+ AVQRI+QGTPPLVVS+VKWLEDELELNAL NP
Sbjct: 721  SVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDELELNALHNP 780

Query: 781  VSRQGTANEKVVLAQRAALATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGI 840
             SR+G  NEKV   QR AL+TALGGRVD++AM+TISGVKATYLLAV+FLEI RF SNGGI
Sbjct: 781  GSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEIIRFISNGGI 840

Query: 841  LNGSISVDASRSAFSCVFEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEA 900
            LNG  SV ASRSAFSCVFEYLKTPNL PAV QCLTA+VHRA+ETAVSWLE RIS  G +A
Sbjct: 841  LNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLEDRISLTGKDA 900

Query: 901  AIRNSILNAHTCFLIKSMCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQ 960
              R     AH CFLIKSM QR++H+R+++V LLTQ RDKFPQV+W+SSCLDSLLFS+H+ 
Sbjct: 901  RNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLDSLLFSVHDN 960

Query: 961  SPTTIVNDPAWVVTVRSLYQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPD 1020
            +P+T+VNDPAW   VRSLYQKVVREWII SLSYAPCTSQGLLQ+ LCKANTWQRAQ T D
Sbjct: 961  TPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANTWQRAQTTTD 1020

Query: 1021 VASLLSEIRIGTGKNDCWTGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSAT 1080
            V SLLSEI+IGTGKN+ W+GI+TANIPAVM+AAAAASG NL +++AFNLEVL TG+ SAT
Sbjct: 1021 VVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEVLGTGVVSAT 1080

Query: 1081 VKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAM 1140
            VKCN+ GEIAGMRRLYN+ GG+QS     T SG G GLQRLI+GAF Q PQ ED+SFN M
Sbjct: 1081 VKCNHAGEIAGMRRLYNSIGGFQSGS---TPSGFGGGLQRLISGAFSQAPQPEDDSFNEM 1140

Query: 1141 LLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLL 1200
            L+ +FV+ LQ+FVN AE GGE++KSQF ETCSQATA LL+NLG +SK+N+EG SQLLRLL
Sbjct: 1141 LIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEGFSQLLRLL 1200

Query: 1201 CWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFS 1260
            CWCPAYIST +AMETG+F+WTWLVSAAPQL  +VLAELVDAW+WTIDTKRGLF+S   +S
Sbjct: 1201 CWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGLFASDVRYS 1260

Query: 1261 GPTAKLRPHLYPGDPEMPPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQ 1320
            GP AKLRPHL PG+PE PPE DPV+QI+AHRLWLGF  DRFEVVR+ S EQLLL GR+LQ
Sbjct: 1261 GPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQLLLLGRMLQ 1320

Query: 1321 GSMELPWKFSHHPAATGSFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWF 1380
             S +L W F+ HPAA G+FF+LM LGLKFCSCQ+Q N+Q F++GLQLLEDR+YR SLGWF
Sbjct: 1321 RSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRIYRTSLGWF 1380

Query: 1381 AFEPEWHDINSTNFAQSEAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDL-NSCHP 1440
            A +PEW+D+N  NF  SEA SV++FV +LSN L + SQ D KG+ RE+GN +D+ +  HP
Sbjct: 1381 AHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESGNLIDVTDQYHP 1440

Query: 1441 VWGQIENYTVGREKRKQLLLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYART 1500
            VWG+++NYT+G+EKRKQLLLMLCQHEADRL+VWAQP+++K++  SR KISSEKW EYA+T
Sbjct: 1441 VWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISSEKWTEYAKT 1500

Query: 1501 AYSVDPRIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLL 1560
            A+SVDPRIA S+ SRFPAN ++++EV+Q VQ++I+D+R IPEALPYFV+PK V+ENSVLL
Sbjct: 1501 AFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPKNVEENSVLL 1560

Query: 1561 QQLPHWAACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDG 1620
            QQLPHWAACSITQA+EFL PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQ+LRYDDG
Sbjct: 1561 QQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQSLRYDDG 1620

Query: 1621 KLVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSF 1680
            +LVEGYLLRA QRSDIF+HILIWH              LQGE  + ET K+ +  KN +F
Sbjct: 1621 RLVEGYLLRATQRSDIFAHILIWH--------------LQGEDVQ-ETPKDGSIDKNAAF 1680

Query: 1681 QALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIE 1740
            Q +LP VRQ IIDGF+  ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGI+RELEKIE
Sbjct: 1681 QEILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIE 1740

Query: 1741 MNGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKV 1800
            M GDDLYLPTAP+KLV+GI+VDSGIPLQSAAKVPIMITFNV DRDGD +DVKPQACIFKV
Sbjct: 1741 MQGDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKV 1800

Query: 1801 GDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGET 1860
            GDDCRQDVLALQVI+LLRD+F+A GLNLYLFPYGVLPTG ERGIIEVVPN RSRSQMGET
Sbjct: 1801 GDDCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGET 1860

Query: 1861 TDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRL 1920
            TDGGLYEIFQQDYGPVGS +FE ARENF+ISSAGYAVASLLLQPKDRHNGNLLFDD+GRL
Sbjct: 1861 TDGGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRL 1920

Query: 1921 IHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAAR 1980
            +HIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ F+SLCVKGYLAAR
Sbjct: 1921 VHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAAR 1980

Query: 1981 RYMDGILTTVALMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTT 2036
            R MDGI++TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VC DAYNKWTT
Sbjct: 1981 RQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTT 2028

BLAST of HG10000440 vs. TAIR 10
Match: AT1G49340.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 2902.5 bits (7523), Expect = 0.0e+00
Identity = 1465/2055 (71.29%), Postives = 1714/2055 (83.41%), Query Frame = 0

Query: 1    MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
            MEAL ELCD+IA+NP  F EK+AWIC RCP  + LL++SPRVSRS LNAVLA+AR +SK 
Sbjct: 1    MEALTELCDIIAKNPKQFSEKLAWICGRCPQTEWLLAESPRVSRSHLNAVLAVARIISKN 60

Query: 61   ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEITPDFSA 120
             +S++ R K+VV EF+ +IP+SF  SFWP ++ +  I+ FY +FL Y+S A +++P+F  
Sbjct: 61   PESIDNRAKSVVNEFLSAIPASFRRSFWPHSFPSQLISSFYCDFLSYLSCAADLSPEFGT 120

Query: 121  EVAVYTGEVVASAISSGGGDS----GISRSFLMVFSKHFLPILPSDANKLVDMVIERYLM 180
            EVA +TGEVV +AI+   GDS     IS++FL+  S+HF  IL SD +KL+ M+++++++
Sbjct: 121  EVARFTGEVVIAAIAPSSGDSDGDPAISKAFLVALSQHFPSILQSDGDKLITMLLDQFVL 180

Query: 181  -MGSGAPREQMQGNSDSSA--PQSIDGSSPRNGSHDSG----SRVGDD------NISSWK 240
                 +P+EQ Q NS +S     S  GS      + SG    +  GD+      N+SS  
Sbjct: 181  NRAPASPKEQRQQNSANSETDTSSSQGSPISTNRYPSGKTEMASPGDEVASHGSNLSSKS 240

Query: 241  SSA-TSYGSGILWKSSADQLGPMFGFNDGGGGGEVLLKQQVSSFEEESLESLEKQAIAFE 300
            SS+    G  I+WKS  DQL   FGF++G GG   + +QQV+SFE+ES+ESLEKQ IAF 
Sbjct: 241  SSSVVMNGGSIVWKSGVDQLS--FGFSEGSGGANPVFRQQVASFEDESIESLEKQEIAFR 300

Query: 301  LLAHILDKARIDSWLWDQVRSIAKKQLQSLLIFVKIRKRDWNEQGSLLKARINAKLLVYQ 360
            L+ HILDK +IDS L DQVR IAK+QLQS+  F+K RKRDWNEQG +LK R+NAKL VYQ
Sbjct: 301  LITHILDKVKIDSKLQDQVRFIAKRQLQSMSAFLKSRKRDWNEQGQVLKTRVNAKLSVYQ 360

Query: 361  AAAKLKLKSLGSLDSEGKVAKKLVFETFALLMDAAEACILSIWRKLRVCEELFSCLLAVL 420
            AAAK+K+KSL SL+++GK +K+LV ET ALL+DAA+AC+ S+WRK++ CEELF  LL+ +
Sbjct: 361  AAAKMKIKSLVSLETDGKTSKRLVLETLALLLDAADACLTSVWRKMKACEELFDSLLSGI 420

Query: 421  GKIAVAWKGQPLRVLLIRLKPLVLTVCAQTDPSDSIHNAMFETVSKTLCEIVESCWTKER 480
             KIAVA  GQPLRVLLIRLKPLVL VCA  D       AM E++ KT C I+ES W K+R
Sbjct: 421  AKIAVARGGQPLRVLLIRLKPLVLAVCALPD-----QGAMLESIFKTSCVIIESAWAKDR 480

Query: 481  APVDTFIMGLTASIRGRNDYEEQVNKEKGVPPLQLNGIRLLANLNVAVKKSEIVDMILPL 540
            APVD FIMGL +SIR RNDYEEQV++EK VP +QLN IRLLA+LNVAVKK E+ DMILPL
Sbjct: 481  APVDNFIMGLASSIRERNDYEEQVDREKQVPAVQLNVIRLLADLNVAVKKPEVADMILPL 540

Query: 541  FIESLEEGDASSPCLLRLQFLDAISRMASLGFEKSYRETVVLMTRSYLSKLSNAASPPPE 600
            FIESLEEGDAS+P  LRLQ LDA+SR+A+LGF+KSYRETVVLMTRSYLSKLS+  S   E
Sbjct: 541  FIESLEEGDASTPSFLRLQLLDAVSRIATLGFDKSYRETVVLMTRSYLSKLSSVGS--VE 600

Query: 601  GSAPAPEATTERLETLPAGFLLIASGLASSKLRSDYRYRLLSLCSDVGLAADSRNARSGA 660
                APEATTER+ETLPAGFL IASGL  +KLRSDYR+RLLSLCSDVGLAA+S++  SG 
Sbjct: 601  SKTSAPEATTERVETLPAGFLTIASGLMDTKLRSDYRHRLLSLCSDVGLAAESKSGGSGV 660

Query: 661  DFLGPLLPAVAEICSDFSPTGDVEPSLLKLFRNLWFYVALFGLAPPI-QNSSVPSKAIST 720
            DFLGPLLPAVAEICSDF PT DVEPSLLKLFRNLWFY+ALFGLAPPI +  + P K+ S 
Sbjct: 661  DFLGPLLPAVAEICSDFDPTMDVEPSLLKLFRNLWFYIALFGLAPPIVKTPTPPLKSTSN 720

Query: 721  TVSSAGSVGTTAIQAVSGPYMWDKQWSSAVQRISQGTPPLVVSTVKWLEDELELNALQNP 780
            +V+S GS+  TA+QAV GPYMWD QW+ AVQRI+QGTPPLVVS+VKWLEDELELNAL NP
Sbjct: 721  SVNSVGSMSATALQAVGGPYMWDNQWALAVQRIAQGTPPLVVSSVKWLEDELELNALHNP 780

Query: 781  VSRQGTANEKVVLAQRAALATALGGRVDISAMSTISGVKATYLLAVSFLEITRFSSNGGI 840
             SR+G  NEKV   QR AL+TALGGRVD++AM+TISGVKATYLLAV+FLEI RF SNGGI
Sbjct: 781  GSRRGNGNEKVASTQRLALSTALGGRVDVAAMNTISGVKATYLLAVAFLEIIRFISNGGI 840

Query: 841  LNGSISVDASRSAFSCVFEYLKTPNLVPAVLQCLTALVHRAYETAVSWLEQRISEIGNEA 900
            LNG  SV ASRSAFSCVFEYLKTPNL PAV QCLTA+VHRA+ETAVSWLE RIS  G +A
Sbjct: 841  LNGESSVSASRSAFSCVFEYLKTPNLTPAVSQCLTAIVHRAFETAVSWLEDRISLTGKDA 900

Query: 901  AIRNSILNAHTCFLIKSMCQREDHIREVAVTLLTQFRDKFPQVMWNSSCLDSLLFSMHNQ 960
              R     AH CFLIKSM QR++H+R+++V LLTQ RDKFPQV+W+SSCLDSLLFS+H+ 
Sbjct: 901  RNRELTTYAHACFLIKSMSQRDEHVRDISVNLLTQLRDKFPQVLWHSSCLDSLLFSVHDN 960

Query: 961  SPTTIVNDPAWVVTVRSLYQKVVREWIIRSLSYAPCTSQGLLQEMLCKANTWQRAQHTPD 1020
            +P+T+VNDPAW   VRSLYQKVVREWII SLSYAPCTSQGLLQ+ LCKANTWQRAQ T D
Sbjct: 961  TPSTVVNDPAWTAAVRSLYQKVVREWIIISLSYAPCTSQGLLQDKLCKANTWQRAQTTTD 1020

Query: 1021 VASLLSEIRIGTGKNDCWTGIQTANIPAVMSAAAAASGGNLNLTKAFNLEVLSTGIGSAT 1080
            V SLLSEI+IGTGKN+ W+GI+TANIPAVM+AAAAASG NL +++AFNLEVL TG+ SAT
Sbjct: 1021 VVSLLSEIKIGTGKNELWSGIRTANIPAVMAAAAAASGANLKVSEAFNLEVLGTGVVSAT 1080

Query: 1081 VKCNYVGEIAGMRRLYNTFGGYQSAGAQPTGSGIGIGLQRLITGAFPQEPQGEDESFNAM 1140
            VKCN+ GEIAGMRRLYN+ GG+QS     T SG G GLQRLI+GAF Q PQ ED+SFN M
Sbjct: 1081 VKCNHAGEIAGMRRLYNSIGGFQSGS---TPSGFGGGLQRLISGAFSQAPQPEDDSFNEM 1140

Query: 1141 LLLKFVQSLQKFVNNAETGGEIDKSQFHETCSQATAFLLANLGTDSKSNIEGLSQLLRLL 1200
            L+ +FV+ LQ+FVN AE GGE++KSQF ETCSQATA LL+NLG +SK+N+EG SQLLRLL
Sbjct: 1141 LIARFVRLLQQFVNTAEKGGEVEKSQFRETCSQATALLLSNLGGESKTNVEGFSQLLRLL 1200

Query: 1201 CWCPAYISTSNAMETGVFVWTWLVSAAPQLGCVVLAELVDAWLWTIDTKRGLFSSREAFS 1260
            CWCPAYIST +AMETG+F+WTWLVSAAPQL  +VLAELVDAW+WTIDTKRGLF+S   +S
Sbjct: 1201 CWCPAYISTPDAMETGIFIWTWLVSAAPQLVSLVLAELVDAWIWTIDTKRGLFASDVRYS 1260

Query: 1261 GPTAKLRPHLYPGDPEMPPEIDPVEQIIAHRLWLGFFFDRFEVVRYQSVEQLLLFGRLLQ 1320
            GP AKLRPHL PG+PE PPE DPV+QI+AHRLWLGF  DRFEVVR+ S EQLLL GR+LQ
Sbjct: 1261 GPAAKLRPHLSPGEPEDPPESDPVDQIVAHRLWLGFLIDRFEVVRHNSAEQLLLLGRMLQ 1320

Query: 1321 GSMELPWKFSHHPAATGSFFTLMFLGLKFCSCQSQVNLQNFKTGLQLLEDRVYRASLGWF 1380
             S +L W F+ HPAA G+FF+LM LGLKFCSCQ+Q N+Q F++GLQLLEDR+YR SLGWF
Sbjct: 1321 RSTDLEWCFTRHPAAAGTFFSLMLLGLKFCSCQTQGNMQKFRSGLQLLEDRIYRTSLGWF 1380

Query: 1381 AFEPEWHDINSTNFAQSEAQSVALFVQYLSNYLKDGSQIDVKGQVRENGNSVDL-NSCHP 1440
            A +PEW+D+N  NF  SEA SV++FV +LSN L + SQ D KG+ RE+GN +D+ +  HP
Sbjct: 1381 AHQPEWYDVNIPNFCHSEALSVSVFVHFLSNELSESSQSDSKGKPRESGNLIDVTDQYHP 1440

Query: 1441 VWGQIENYTVGREKRKQLLLMLCQHEADRLEVWAQPVNTKETTSSRPKISSEKWVEYART 1500
            VWG+++NYT+G+EKRKQLLLMLCQHEADRL+VWAQP+++K++  SR KISSEKW EYA+T
Sbjct: 1441 VWGEMDNYTLGKEKRKQLLLMLCQHEADRLDVWAQPISSKDSPYSRLKISSEKWTEYAKT 1500

Query: 1501 AYSVDPRIAFSLVSRFPANVTLRAEVSQQVQSHILDIRCIPEALPYFVSPKAVDENSVLL 1560
            A+SVDPRIA S+ SRFPAN ++++EV+Q VQ++I+D+R IPEALPYFV+PK V+ENSVLL
Sbjct: 1501 AFSVDPRIALSVASRFPANASVKSEVTQLVQTNIVDLRTIPEALPYFVTPKNVEENSVLL 1560

Query: 1561 QQLPHWAACSITQAIEFLAPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQALRYDDG 1620
            QQLPHWAACSITQA+EFL PAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQ+LRYDDG
Sbjct: 1561 QQLPHWAACSITQALEFLTPAYKGHPRVMAYVLRVLESYPPERVTFFMPQLVQSLRYDDG 1620

Query: 1621 KLVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSF 1680
            +LVEGYLLRA QRSDIF+HILIWH              LQGE  + ET K+ +  KN +F
Sbjct: 1621 RLVEGYLLRATQRSDIFAHILIWH--------------LQGEDVQ-ETPKDGSIDKNAAF 1680

Query: 1681 QALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIE 1740
            Q +LP VRQ IIDGF+  ALD+F REFDFFDKVTSISGVLFPLPK+ERRAGI+RELEKIE
Sbjct: 1681 QEILPQVRQHIIDGFSPNALDMFTREFDFFDKVTSISGVLFPLPKEERRAGIRRELEKIE 1740

Query: 1741 MNGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKV 1800
            M GDDLYLPTAP+KLV+GI+VDSGIPLQSAAKVPIMITFNV DRDGD +DVKPQACIFKV
Sbjct: 1741 MQGDDLYLPTAPNKLVRGIRVDSGIPLQSAAKVPIMITFNVIDRDGDHSDVKPQACIFKV 1800

Query: 1801 GDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGET 1860
            GDDCRQDVLALQVI+LLRD+F+A GLNLYLFPYGVLPTG ERGIIEVVPN RSRSQMGET
Sbjct: 1801 GDDCRQDVLALQVISLLRDIFQAAGLNLYLFPYGVLPTGAERGIIEVVPNTRSRSQMGET 1860

Query: 1861 TDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRL 1920
            TDGGLYEIFQQDYGPVGS +FE ARENF+ISSAGYAVASLLLQPKDRHNGNLLFDD+GRL
Sbjct: 1861 TDGGLYEIFQQDYGPVGSTTFETARENFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRL 1920

Query: 1921 IHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAAR 1980
            +HIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKS+TW+ F+SLCVKGYLAAR
Sbjct: 1921 VHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAAR 1980

Query: 1981 RYMDGILTTVALMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTT 2036
            R MDGI++TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA+FMI VC DAYNKWTT
Sbjct: 1981 RQMDGIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAAHFMIHVCTDAYNKWTT 2028

BLAST of HG10000440 vs. TAIR 10
Match: AT1G51040.1 (Protein kinase superfamily protein )

HSP 1 Score: 688.7 bits (1776), Expect = 1.4e-197
Identity = 344/434 (79.26%), Postives = 375/434 (86.41%), Query Frame = 0

Query: 1602 LVEGYLLRAAQRSDIFSHILIWHLQVFIVYSNRLSFDLQGETCEPETGKEATSTKNNSFQ 1661
            +VEGYLLRAAQRSDIF+H LIWH              LQGE+ E E  K+    KN SFQ
Sbjct: 115  MVEGYLLRAAQRSDIFAHTLIWH--------------LQGESVE-EIVKDGAFDKNASFQ 174

Query: 1662 ALLPVVRQQIIDGFTGKALDVFQREFDFFDKVTSISGVLFPLPKDERRAGIQRELEKIEM 1721
             +L  VRQ I+DGFT KAL+ F REFDFF+KVTSISG L PLPK+ER AGI+RELEKI+M
Sbjct: 175  EILSDVRQHIVDGFTPKALNFFSREFDFFEKVTSISGALLPLPKEERVAGIRRELEKIKM 234

Query: 1722 NGDDLYLPTAPSKLVKGIQVDSGIPLQSAAKVPIMITFNVADRDGDPNDVKPQACIFKVG 1781
             G+DLYLPTAP+KLV+ IQVDSGIPLQSAAKVPIMITFNV D DGD NDVKPQACIFKVG
Sbjct: 235  QGEDLYLPTAPNKLVRVIQVDSGIPLQSAAKVPIMITFNVVDLDGDHNDVKPQACIFKVG 294

Query: 1782 DDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRSRSQMGETT 1841
            DDCRQDVLALQVI+LL D+F+AVGLNLYLFPYGVLPT        VVPN RSRSQMGETT
Sbjct: 295  DDCRQDVLALQVISLLGDIFQAVGLNLYLFPYGVLPT--------VVPNTRSRSQMGETT 354

Query: 1842 DGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHNGNLLFDDLGRLI 1901
            DGGLYEIFQQ+YG VGS +FE AR NF+ISSAGYAVASLLLQPKDRHNGNLLFDD+GRL+
Sbjct: 355  DGGLYEIFQQNYGLVGSTTFETARANFLISSAGYAVASLLLQPKDRHNGNLLFDDVGRLV 414

Query: 1902 HIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGVMKSETWNLFLSLCVKGYLAARR 1961
            HIDFGFILETSPGGNMRFE+AHFKLSHEMTQLLDPSGVMKS+TW+ F+SLCVKGYLAARR
Sbjct: 415  HIDFGFILETSPGGNMRFENAHFKLSHEMTQLLDPSGVMKSKTWHQFVSLCVKGYLAARR 474

Query: 1962 YMDGILTTVALMLDSGLPCFSRGDPIGNLRKRFHPEMSEREAANFMIRVCVDAYNKWTTA 2021
            YMD I++TV +ML+SGLPCFSRGDPIGNLRKRFHPEMSEREAA FMI VC DAYNKWTTA
Sbjct: 475  YMDEIISTVQMMLESGLPCFSRGDPIGNLRKRFHPEMSEREAALFMINVCTDAYNKWTTA 525

Query: 2022 GYDLIQYLQQGIEK 2036
            GYDLIQYLQQG+EK
Sbjct: 535  GYDLIQYLQQGVEK 525

HSP 2 Score: 125.2 bits (313), Expect = 6.1e-28
Identity = 56/114 (49.12%), Postives = 79/114 (69.30%), Query Frame = 0

Query: 1   MEALLELCDLIAQNPSAFFEKIAWICSRCPPPDALLSDSPRVSRSQLNAVLAIARFLSKC 60
           ME L ELCD+I QNP  F +K+ WIC +CP    L ++SPR S+S LNA+LA+ R LSK 
Sbjct: 1   MERLNELCDIIEQNPKQFLDKLPWICQQCPQSKLLRAESPRFSQSHLNAILAVTRILSKI 60

Query: 61  ADSVEPRPKAVVLEFIRSIPSSFNHSFWPPTYGNDSITLFYTEFLGYVSKATEI 115
            D+ +   K VVL+F++++P SF+ SFWP +   +SI+ FY  FLGYVS  +++
Sbjct: 61  VDTTDENAKFVVLDFLQTVPKSFHRSFWPYSLSLESISAFYCSFLGYVSCLSQL 114

BLAST of HG10000440 vs. TAIR 10
Match: AT5G64070.1 (phosphatidylinositol 4-OH kinase beta1 )

HSP 1 Score: 206.5 bits (524), Expect = 2.1e-52
Identity = 111/266 (41.73%), Postives = 163/266 (61.28%), Query Frame = 0

Query: 1774 QACIFKVGDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRS 1833
            ++ I K GDDCRQ+ LA+Q+I+   D+F+  GL L+L PY VL T     +IE +P+  S
Sbjct: 859  RSIIVKSGDDCRQEHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 918

Query: 1834 ----RSQMGETTDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHN 1893
                +S+    T   L + F   Y    SPSF+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 919  IHSIKSRYPNIT--SLRDFFVAKY-KENSPSFKLAQRNFVESMAGYSLVCYLLQVKDRHN 978

Query: 1894 GNLLFDDLGRLIHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLD-PSGVMKSETWNLF 1953
            GNLL D+ G +IHIDFGF+L  SPGG + FESA FKL+ E+ +++D  +  + SE ++ F
Sbjct: 979  GNLLLDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDADGVPSEFFDYF 1038

Query: 1954 LSLCVKGYLAARRYMDGILTTVALMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFM 2013
              LC++G+L  R++ + I+  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +
Sbjct: 1039 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1098

Query: 2014 IRVCVDAYNKWTTAGYDLIQYLQQGI 2034
            + +   + + W T  YD  Q +  GI
Sbjct: 1099 LSLISSSLDAWRTRQYDYYQRVLNGI 1120

BLAST of HG10000440 vs. TAIR 10
Match: AT5G09350.1 (phosphatidylinositol 4-OH kinase beta2 )

HSP 1 Score: 205.7 bits (522), Expect = 3.6e-52
Identity = 110/266 (41.35%), Postives = 162/266 (60.90%), Query Frame = 0

Query: 1774 QACIFKVGDDCRQDVLALQVIALLRDVFEAVGLNLYLFPYGVLPTGPERGIIEVVPNVRS 1833
            ++ I K GDDCRQ+ LA+Q+I+   D+F+  GL L+L PY VL T     +IE +P+  S
Sbjct: 854  RSIIVKSGDDCRQEHLAVQLISHFFDIFQEAGLPLWLRPYEVLVTSSYTALIETIPDTAS 913

Query: 1834 ----RSQMGETTDGGLYEIFQQDYGPVGSPSFEAARENFIISSAGYAVASLLLQPKDRHN 1893
                +S+    T   L + F   +    SPSF+ A+ NF+ S AGY++   LLQ KDRHN
Sbjct: 914  IHSIKSRYPNIT--SLRDFFDAKF-KENSPSFKLAQRNFVESMAGYSLVCYLLQIKDRHN 973

Query: 1894 GNLLFDDLGRLIHIDFGFILETSPGGNMRFESAHFKLSHEMTQLLDPSGV-MKSETWNLF 1953
            GNLL D+ G +IHIDFGF+L  SPGG + FESA FKL+ E+ +++D     + SE ++ F
Sbjct: 974  GNLLMDEEGHIIHIDFGFMLSNSPGG-VNFESAPFKLTRELLEVMDSDAEGLPSEFFDYF 1033

Query: 1954 LSLCVKGYLAARRYMDGILTTVALMLDSGLPCFSRGD-PIGNLRKRFHPEMSEREAANFM 2013
              LC++G+L  R++ + I+  V ++ DSG PCF  G   I NLRKRFH  ++E +  + +
Sbjct: 1034 KVLCIQGFLTCRKHAERIILLVEMLQDSGFPCFKGGPRTIQNLRKRFHLSLTEEQCVSLV 1093

Query: 2014 IRVCVDAYNKWTTAGYDLIQYLQQGI 2034
            + +   + + W T  YD  Q +  GI
Sbjct: 1094 LSLISSSLDAWRTRQYDYYQRVLNGI 1115