HG10000383 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000383
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein, putative
LocationChr09: 4557742 .. 4558714 (-)
RNA-Seq ExpressionHG10000383
SyntenyHG10000383
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCCAACAAGAAGAAATCATTTTCGAATTCCTCCCGGTTTTCCGCGTCTTCAAAAACGGCCGCGTCGACCGCCTCCTTCTCACCGACCGCGTTCCTCCGTCCACCGATCAACTCACCGGAGTCCAATCCAAAGACTTTCTCATCGATCCACAAACCGGAGTCTCCGTCCGTCTCTACCTCCCCAAACTTTCAGATTCCACCATAAAGCTTCCGCTTCTAATCTACATCCACGGCGGCGCTTTCTCCGTCGGCTCCCCTGCCTCTCCGATGTTCCACAACCATCTGATCTCCCTCACCGCCCAAGCAAACGCCGTCGCTCTCTCCGTCGACTACCGCCTAGCACCAGAGAATAATCTCCCGGCGGCATACGACGACGCTTGGACAGCGGTGGAATGGGCGGCGTCGCACTCGAATCGCAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTGAATCGCGTGTTTGTGGTAGGGGAGAGCGCTGGGGCCAATATTTCGTACAATGTAATGGGCCGGGCCAGCGAAAATGAAAATGGGCTTAATTGAATGAAGATAATTGGGCTGGGGCTTGTGCATCCGTATTTCATGATCGACATGCCCGATAGGTTGATAGAGTATTTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGACTGAACCCGAAATCCAACCCGAAACTAGGAAAGTTCCGGTGCAGCGGCGTCCTTATATGCGCTGCGGAAATTGATCCTTTGAAATATGGGGCGGTTGGTGTTCACGCGGCTTTGAAGGAGAGTGGGTTTAAAGGGTCGGTGGAGATGATGGTGAGCGAGGGAGAGGACCATGGTTTCCATCTCTTTATTCCCAACACTGAGAAGGCTCAAGCATTTGGTTACAAAGCTAGCTTCCTTTATCCAATCAACATTACCATAACTACTGTAGTTTGTTTATTTATTTTTCATACTAAGAAAATTTTCCTAGTCTAA

mRNA sequence

ATGGATTCCCAACAAGAAGAAATCATTTTCGAATTCCTCCCGGTTTTCCGCGTCTTCAAAAACGGCCGCGTCGACCGCCTCCTTCTCACCGACCGCGTTCCTCCGTCCACCGATCAACTCACCGGAGTCCAATCCAAAGACTTTCTCATCGATCCACAAACCGGAGTCTCCGTCCGTCTCTACCTCCCCAAACTTTCAGATTCCACCATAAAGCTTCCGCTTCTAATCTACATCCACGGCGGCGCTTTCTCCGTCGGCTCCCCTGCCTCTCCGATGTTCCACAACCATCTGATCTCCCTCACCGCCCAAGCAAACGCCGTCGCTCTCTCCGTCGACTACCGCCTAGCACCAGAGAATAATCTCCCGGCGGCATACGACGACGCTTGGACAGCGGTGGAATGGGCGGCGTCGCACTCGAATCGCAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTGAATCGCGTGTTTGTGGTAGGGGAGAGCGCTGGGGCCAATATTTCGTACAATAGTATTTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGACTGAACCCGAAATCCAACCCGAAACTAGGAAAGTTCCGGTGCAGCGGCGTCCTTATATGCGCTGCGGAAATTGATCCTTTGAAATATGGGGCGGTTGGTGTTCACGCGGCTTTGAAGGAGAGTGGGTTTAAAGGGTCGGTGGAGATGATGGTGAGCGAGGGAGAGGACCATGGTTTCCATCTCTTTATTCCCAACACTGAGAAGGCTCAAGCATTTGGTTACAAAGCTAGCTTCCTTTATCCAATCAACATTACCATAACTACTGTAGTTTGTTTATTTATTTTTCATACTAAGAAAATTTTCCTAGTCTAA

Coding sequence (CDS)

ATGGATTCCCAACAAGAAGAAATCATTTTCGAATTCCTCCCGGTTTTCCGCGTCTTCAAAAACGGCCGCGTCGACCGCCTCCTTCTCACCGACCGCGTTCCTCCGTCCACCGATCAACTCACCGGAGTCCAATCCAAAGACTTTCTCATCGATCCACAAACCGGAGTCTCCGTCCGTCTCTACCTCCCCAAACTTTCAGATTCCACCATAAAGCTTCCGCTTCTAATCTACATCCACGGCGGCGCTTTCTCCGTCGGCTCCCCTGCCTCTCCGATGTTCCACAACCATCTGATCTCCCTCACCGCCCAAGCAAACGCCGTCGCTCTCTCCGTCGACTACCGCCTAGCACCAGAGAATAATCTCCCGGCGGCATACGACGACGCTTGGACAGCGGTGGAATGGGCGGCGTCGCACTCGAATCGCAACGGCCCTGAGATTTGGCTGAACGAGCATGCGGATTTGAATCGCGTGTTTGTGGTAGGGGAGAGCGCTGGGGCCAATATTTCGTACAATAGTATTTTCCCGACGTCGGCGGGTCTGGAGGATCCGAGACTGAACCCGAAATCCAACCCGAAACTAGGAAAGTTCCGGTGCAGCGGCGTCCTTATATGCGCTGCGGAAATTGATCCTTTGAAATATGGGGCGGTTGGTGTTCACGCGGCTTTGAAGGAGAGTGGGTTTAAAGGGTCGGTGGAGATGATGGTGAGCGAGGGAGAGGACCATGGTTTCCATCTCTTTATTCCCAACACTGAGAAGGCTCAAGCATTTGGTTACAAAGCTAGCTTCCTTTATCCAATCAACATTACCATAACTACTGTAGTTTGTTTATTTATTTTTCATACTAAGAAAATTTTCCTAGTCTAA

Protein sequence

MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIFPTSAGLEDPRLNPKSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQAFGYKASFLYPINITITTVVCLFIFHTKKIFLV
Homology
BLAST of HG10000383 vs. NCBI nr
Match: XP_038900484.1 (probable carboxylesterase 2 [Benincasa hispida] >XP_038900485.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900486.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900487.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900488.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900490.1 probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-97
Identity = 188/295 (63.73%), Postives = 222/295 (75.25%), Query Frame = 0

Query: 6   EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYLPKL 65
           +E++FEFLPVFRVF++GRV+RL  TDR+PPSTD LTGVQSKD LIDPQT +SVR+YLP +
Sbjct: 9   DEVVFEFLPVFRVFQSGRVERLFPTDRLPPSTDPLTGVQSKDILIDPQTQLSVRIYLPHI 68

Query: 66  SDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLPAAY 125
           SDST KLPLLIYIHGGAF++GS ASP++H HLISL A++NAVA+SV+YRLAPEN LPAAY
Sbjct: 69  SDSTKKLPLLIYIHGGAFAIGSVASPIYHPHLISLAAESNAVAVSVEYRLAPENPLPAAY 128

Query: 126 DDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------------- 185
           DDAW AVEWAASHSN +G EIWLN+HAD +RVFV+G+SAGANISYN              
Sbjct: 129 DDAWAAVEWAASHSNGDGAEIWLNQHADFSRVFVIGDSAGANISYNVVDRASESDNGPKG 188

Query: 186 -----------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVLICAAEI 245
                                   +FPTSAGLEDPR+NP +NP+LGK RC  VLI AAE+
Sbjct: 189 MKIIGLGLVHPFFMIDKPDKLIEYVFPTSAGLEDPRMNPWANPRLGKLRCERVLILAAEL 248

Query: 246 DPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQAFGYK-ASF 263
           D LK  A G+HAAL+ESGF+GSVE+  +EG+DH FHL  P TEKA A   K ASF
Sbjct: 249 DSLKDRARGIHAALRESGFEGSVEIEETEGKDHVFHLLKPETEKAVALVKKIASF 303

BLAST of HG10000383 vs. NCBI nr
Match: XP_023532126.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.1 bits (910), Expect = 5.5e-94
Identity = 189/302 (62.58%), Postives = 220/302 (72.85%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAFS+GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFSIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAW AVEW ASHSNR+G E+WLNEHAD +RVF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP LG+FRC+ VL
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQAFGYK-AS 264
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+ EKA A   K AS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of HG10000383 vs. NCBI nr
Match: XP_023007510.1 (probable carboxylesterase 2 [Cucurbita maxima])

HSP 1 Score: 350.9 bits (899), Expect = 1.0e-92
Identity = 187/302 (61.92%), Postives = 219/302 (72.52%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAF++GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAWTAVEW ASHSNR+G E+WLNEHAD + VF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP L +FRC+ VL
Sbjct: 181 DNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQAFGYK-AS 264
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+ EKA A   K AS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of HG10000383 vs. NCBI nr
Match: KAG6605131.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 350.1 bits (897), Expect = 1.8e-92
Identity = 183/293 (62.46%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAF++GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAW AVEW ASHSNR+G E+WLNEHAD +RVF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP LG+ RC+ VL
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQA 256
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+ EKA A
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVA 293

BLAST of HG10000383 vs. NCBI nr
Match: XP_022948164.1 (probable carboxylesterase 2 [Cucurbita moschata])

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-92
Identity = 183/293 (62.46%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAF++GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAW AVEW ASHSNR+G E+WLNEHAD +RVF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP LG+FRC+ VL
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQA 256
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+  KA A
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVA 293

BLAST of HG10000383 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.3e-50
Identity = 115/308 (37.34%), Postives = 161/308 (52.27%), Query Frame = 0

Query: 5   QEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYLPK 64
           + EI  EFLP  R++K+GRV+RL+ TD +P S D    V SKD +  P+  +SVRL+LP 
Sbjct: 2   ESEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPH 61

Query: 65  LSDSTI---KLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNL 124
            S       KLPLLIYIHGGA+ + SP SP++HN+L  +   AN +A+SV YR APE+ +
Sbjct: 62  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 121

Query: 125 PAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF------- 184
           PAAY+D W+A++W  +HSN +GP  W+N+HAD  +VF+ G+SAG NIS++          
Sbjct: 122 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK 181

Query: 185 --------------------------------------------PTSA-GLEDPRLNPK- 244
                                                       P S  G +DP  N   
Sbjct: 182 LDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNG 241

Query: 245 SNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIP 257
           S        C  VL+  A  D      +   A L++  ++G+VE++  EGEDH FHL  P
Sbjct: 242 SGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNP 301

BLAST of HG10000383 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 5.3e-47
Identity = 115/317 (36.28%), Postives = 170/317 (53.63%), Query Frame = 0

Query: 6   EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPS-TDQLTGVQSKDFLIDPQTGVSVRLYLPK 65
           +EI  E  P+ RV+K+G V+R L +  VPPS  D  TGV +KD +I     +S R+YLPK
Sbjct: 11  KEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISARVYLPK 70

Query: 66  LSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLPAA 125
           L+++T KLP+L+Y HGGAF + S  S +   +L  + ++AN + +S++YRLAPE+ LPAA
Sbjct: 71  LNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAA 130

Query: 126 YDDAWTAVEWAASHS-NRNGP---EIWLNEHADLNRVFVVGESAGANISYN--------- 185
           Y+D W A++W  SHS N N P   + WL +H D NR ++ G+++GANI++N         
Sbjct: 131 YEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEA 190

Query: 186 -----------SIFP-------------------------------TSAGLEDPRLNP-- 245
                      S FP                                  G+++P +NP  
Sbjct: 191 LPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGIDNPLINPLA 250

Query: 246 KSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFI 264
              P L    C  +L+  A  D L+   +  + A+KESG+KG VE+   EGE+H F ++ 
Sbjct: 251 PGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEEHCFQIYH 310

BLAST of HG10000383 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.0e-47
Identity = 108/303 (35.64%), Postives = 164/303 (54.13%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           M+S ++++  E LP   V  +G V+RL  T+  PP  D +TGV SKD +I+P+TG+S R+
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENN 120
           Y P       K+PL++Y HGGAF + S + P +H  L  +  QAN +A+SV+YRLAPE+ 
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF------ 180
           LP AY+D+WTA++   + +     E W+N++ADL+ +F+VG+SAGANIS++  F      
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 ------------------------------------------PTSAGLEDPRLNP--KSN 240
                                                     P+  G +DP +NP    +
Sbjct: 181 QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 240

Query: 241 PKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNT 254
           P LG   C  V+I  AE D L       +  L +S +KG VE+M ++ +DH FH+F P+ 
Sbjct: 241 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 298

BLAST of HG10000383 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.6e-46
Identity = 113/320 (35.31%), Postives = 163/320 (50.94%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           MDS   EI  +  P+ +++K+GR++RL+    VPPS++   GV SKD +      +SVR+
Sbjct: 1   MDS---EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRI 60

Query: 61  YLPK--LSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPE 120
           YLP+   +++  KLPLL+Y HGG F + +  SP +H  L +  + +N VA+SVDYR APE
Sbjct: 61  YLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPE 120

Query: 121 NNLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF---- 180
           + +   +DD+WTA++W  +H   +G E WLN+HAD +RVF+ G+SAGANI ++       
Sbjct: 121 HPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAK 180

Query: 181 --------------------------------------------------PTSA-GLEDP 240
                                                             P S  G +DP
Sbjct: 181 EKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDP 240

Query: 241 RLN--PKSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDH 262
            LN     +  L    C  VL+  AE D L     G  A L++SG+KG VE++ SEGEDH
Sbjct: 241 LLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDH 300

BLAST of HG10000383 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.5e-46
Identity = 109/307 (35.50%), Postives = 163/307 (53.09%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           MDS   EI F++ P FR+FKNG ++RL+    VPPS +   GV SKD +  P+  +S+R+
Sbjct: 1   MDS---EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRI 60

Query: 61  YLPKLS---DSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAP 120
           YLP+ S       K+PLL+Y HGG F + +  SP++H  L S  +  + +A+SV+YR AP
Sbjct: 61  YLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAP 120

Query: 121 ENNLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF--- 180
           E+ +P  Y+D+W A++W  +H  R+GPE WLN+HAD ++VF+ G+SAGANI+++      
Sbjct: 121 EHPIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVD 180

Query: 181 ----------------------------------------------PTSA-GLEDPRLNP 240
                                                         P S  G+EDP +N 
Sbjct: 181 KEKLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV 240

Query: 241 KSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFI 255
             +   G   C  VL+  A  D L  G     A L++SG+ G V++M ++ E H FHL  
Sbjct: 241 VGSDLTG-LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 300

BLAST of HG10000383 vs. ExPASy TrEMBL
Match: A0A6J1L361 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 5.0e-93
Identity = 187/302 (61.92%), Postives = 219/302 (72.52%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAF++GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAWTAVEW ASHSNR+G E+WLNEHAD + VF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP L +FRC+ VL
Sbjct: 181 DNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQAFGYK-AS 264
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+ EKA A   K AS
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKVAS 300

BLAST of HG10000383 vs. ExPASy TrEMBL
Match: A0A6J1G929 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.1e-92
Identity = 183/293 (62.46%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 1   MDSQQ-EEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   EE++F+FL VFRVFK+GRV+RL+  D VPPSTD  TGVQSKD LIDP T +SVR
Sbjct: 1   MDSTAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLPK+S S  KLPLLIYIHGGAF++GS AS ++H+HLISL A+ANAVA+SV+YRLAPEN
Sbjct: 61  IYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPEN 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAW AVEW ASHSNR+G E+WLNEHAD +RVF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRASEN 180

Query: 181 -----------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVL 240
                                         IFPTS GLEDPR+NP +NP LG+FRC+ VL
Sbjct: 181 DNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNRVL 240

Query: 241 ICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKAQA 256
           I AAE D L   A G HAAL+ESG+KGSVEM+ +EGEDH FHLF P+  KA A
Sbjct: 241 IIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVA 293

BLAST of HG10000383 vs. ExPASy TrEMBL
Match: A0A6J1D5P6 (probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.5e-92
Identity = 181/288 (62.85%), Postives = 214/288 (74.31%), Query Frame = 0

Query: 1   MDSQ-QEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVR 60
           MDS   +E++FEF PVFRVF++GRV+RL+  DRVPPSTDQ TGVQSKD LIDPQTGVSVR
Sbjct: 1   MDSDAADEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVSVR 60

Query: 61  LYLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPEN 120
           +YLP + DST KLPLLIYIHGGAF++GS  S ++H HLISL A+ANAVALSV+YRLAPE+
Sbjct: 61  IYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEH 120

Query: 121 NLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN-------- 180
            LPAAYDDAWT+VEWAASH+NR+G EIWLN HAD +RVF+VG+SAGANISYN        
Sbjct: 121 PLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAGNG 180

Query: 181 --------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVLICA 240
                                      IFPTSAGLEDPR+NP++NP+L   RC+ V+I  
Sbjct: 181 PAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQVVILV 240

Query: 241 AEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKA 254
           AE D LK    G HAAL+ESG+KGSVE++ +EGE H FHLF P  EKA
Sbjct: 241 AENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKA 287

BLAST of HG10000383 vs. ExPASy TrEMBL
Match: A0A6P5TIN3 (probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 3.4e-73
Identity = 143/286 (50.00%), Postives = 191/286 (66.78%), Query Frame = 0

Query: 3   SQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYL 62
           S   EI  EF  +FR++K+GRV+RL  T+ VPPSTD  TGV+SKD ++ P++G+S R++L
Sbjct: 5   SSSTEISVEFPTIFRIYKDGRVERLKGTETVPPSTDLETGVRSKDVVLSPESGLSARIFL 64

Query: 63  PKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLP 122
           PKL D T KLPLL++IHGGAF + SP SP++HNH   LT++AN V LSV+YR APE+ LP
Sbjct: 65  PKLPDPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLP 124

Query: 123 AAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN----------- 182
            AY+D+W A++WAA+HSNRNGPEIWLN++ D +RVFV G+SAGA ++++           
Sbjct: 125 VAYEDSWEALQWAAAHSNRNGPEIWLNDYVDFDRVFVGGDSAGATLTHHVVHRAGVHGLS 184

Query: 183 ------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVLICAAE 242
                                    +FP   GLEDPR+NP  +PKLG+  C  VLI  AE
Sbjct: 185 GAKIVGMILFHPFFGNEKPDKLLEVVFPACNGLEDPRVNPGKDPKLGEIGCGRVLIFVAE 244

Query: 243 IDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKA 254
            D L+  A   + ALK+SG+ G VE++ SEGEDH FHLF P+ +KA
Sbjct: 245 KDFLRDRAWAYYEALKKSGWGGMVEILESEGEDHVFHLFNPSCDKA 290

BLAST of HG10000383 vs. ExPASy TrEMBL
Match: A0A314Y2B2 (Putative carboxylesterase 5 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_20876 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 2.9e-72
Identity = 141/286 (49.30%), Postives = 191/286 (66.78%), Query Frame = 0

Query: 3   SQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYL 62
           S   EI  EF  +FR++K+GRV+RL  T+ VPPSTD  TGV+SKD ++ P++G+S R++L
Sbjct: 5   SSSTEISVEFPTIFRIYKDGRVERLKGTETVPPSTDPETGVRSKDIVLSPESGLSARIFL 64

Query: 63  PKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLP 122
           PKL+D T KLPLL++IHGGAF + SP SP++HNH   LT++AN V LSV+YR APE+ LP
Sbjct: 65  PKLTDPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLP 124

Query: 123 AAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYN----------- 182
           AAY+D+W A++WAA+HSNRNGPE WLN++ D +RVFV G+SAGA ++++           
Sbjct: 125 AAYEDSWEALQWAAAHSNRNGPETWLNDYVDFDRVFVGGDSAGATLTHHVVHRAGIHGLS 184

Query: 183 ------------------------SIFPTSAGLEDPRLNPKSNPKLGKFRCSGVLICAAE 242
                                    +FP   GL+DPR+NP  +PKL +  C  VLI  AE
Sbjct: 185 GAKIVGMILFHPFFGNGKPDKLLEVVFPACNGLDDPRVNPGKDPKLREIGCGRVLIFVAE 244

Query: 243 IDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTEKA 254
            D L+  A   + ALK+SG+ G VE++ SEGEDH FHLF P+ +KA
Sbjct: 245 KDFLRDRAWAYYEALKKSGWGGMVEILESEGEDHVFHLFNPSCDKA 290

BLAST of HG10000383 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 200.7 bits (509), Expect = 1.6e-51
Identity = 115/308 (37.34%), Postives = 161/308 (52.27%), Query Frame = 0

Query: 5   QEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYLPK 64
           + EI  EFLP  R++K+GRV+RL+ TD +P S D    V SKD +  P+  +SVRL+LP 
Sbjct: 2   ESEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPH 61

Query: 65  LSDSTI---KLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNL 124
            S       KLPLLIYIHGGA+ + SP SP++HN+L  +   AN +A+SV YR APE+ +
Sbjct: 62  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPV 121

Query: 125 PAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF------- 184
           PAAY+D W+A++W  +HSN +GP  W+N+HAD  +VF+ G+SAG NIS++          
Sbjct: 122 PAAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK 181

Query: 185 --------------------------------------------PTSA-GLEDPRLNPK- 244
                                                       P S  G +DP  N   
Sbjct: 182 LDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNG 241

Query: 245 SNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIP 257
           S        C  VL+  A  D      +   A L++  ++G+VE++  EGEDH FHL  P
Sbjct: 242 SGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNP 301

BLAST of HG10000383 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 6.4e-48
Identity = 108/303 (35.64%), Postives = 164/303 (54.13%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           M+S ++++  E LP   V  +G V+RL  T+  PP  D +TGV SKD +I+P+TG+S R+
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  YLPKLSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENN 120
           Y P       K+PL++Y HGGAF + S + P +H  L  +  QAN +A+SV+YRLAPE+ 
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 LPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF------ 180
           LP AY+D+WTA++   + +     E W+N++ADL+ +F+VG+SAGANIS++  F      
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 ------------------------------------------PTSAGLEDPRLNP--KSN 240
                                                     P+  G +DP +NP    +
Sbjct: 181 QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 240

Query: 241 PKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNT 254
           P LG   C  V+I  AE D L       +  L +S +KG VE+M ++ +DH FH+F P+ 
Sbjct: 241 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 298

BLAST of HG10000383 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.9e-47
Identity = 113/320 (35.31%), Postives = 163/320 (50.94%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           MDS   EI  +  P+ +++K+GR++RL+    VPPS++   GV SKD +      +SVR+
Sbjct: 1   MDS---EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRI 60

Query: 61  YLPK--LSDSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPE 120
           YLP+   +++  KLPLL+Y HGG F + +  SP +H  L +  + +N VA+SVDYR APE
Sbjct: 61  YLPEKAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPE 120

Query: 121 NNLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF---- 180
           + +   +DD+WTA++W  +H   +G E WLN+HAD +RVF+ G+SAGANI ++       
Sbjct: 121 HPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAK 180

Query: 181 --------------------------------------------------PTSA-GLEDP 240
                                                             P S  G +DP
Sbjct: 181 EKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDP 240

Query: 241 RLN--PKSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDH 262
            LN     +  L    C  VL+  AE D L     G  A L++SG+KG VE++ SEGEDH
Sbjct: 241 LLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDH 300

BLAST of HG10000383 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-47
Identity = 109/307 (35.50%), Postives = 163/307 (53.09%), Query Frame = 0

Query: 1   MDSQQEEIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRL 60
           MDS   EI F++ P FR+FKNG ++RL+    VPPS +   GV SKD +  P+  +S+R+
Sbjct: 1   MDS---EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRI 60

Query: 61  YLPKLS---DSTIKLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAP 120
           YLP+ S       K+PLL+Y HGG F + +  SP++H  L S  +  + +A+SV+YR AP
Sbjct: 61  YLPQNSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAP 120

Query: 121 ENNLPAAYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNSIF--- 180
           E+ +P  Y+D+W A++W  +H  R+GPE WLN+HAD ++VF+ G+SAGANI+++      
Sbjct: 121 EHPIPTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVD 180

Query: 181 ----------------------------------------------PTSA-GLEDPRLNP 240
                                                         P S  G+EDP +N 
Sbjct: 181 KEKLPPENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV 240

Query: 241 KSNPKLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFI 255
             +   G   C  VL+  A  D L  G     A L++SG+ G V++M ++ E H FHL  
Sbjct: 241 VGSDLTG-LGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 300

BLAST of HG10000383 vs. TAIR 10
Match: AT1G49650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.4e-47
Identity = 114/305 (37.38%), Postives = 155/305 (50.82%), Query Frame = 0

Query: 7   EIIFEFLPVFRVFKNGRVDRLLLTDRVPPSTDQLTGVQSKDFLIDPQTGVSVRLYLPKLS 66
           EII E  P  RV+K+GR++RL  T+ VP S +    V SKD +  P   +SVRL+LP  S
Sbjct: 60  EIISEHPPFVRVYKDGRIERLSGTETVPASLNPRNDVVSKDVVYSPGHNLSVRLFLPHKS 119

Query: 67  DSTI---KLPLLIYIHGGAFSVGSPASPMFHNHLISLTAQANAVALSVDYRLAPENNLPA 126
                  KLPLLIY HGGA+   SP SP++HN L  +   AN +A+SV YR APE+ +PA
Sbjct: 120 TQLAAGNKLPLLIYFHGGAWINESPFSPIYHNFLTEVVKSANCLAVSVQYRRAPEDPVPA 179

Query: 127 AYDDAWTAVEWAASHSNRNGPEIWLNEHADLNRVFVVGESAGANISYNS----------- 186
           AY+D W+A++W  SHS  +G E W+N++AD  RVF+ G+SAG NIS++            
Sbjct: 180 AYEDTWSAIQWIFSHSCGSGEEDWINKYADFERVFLAGDSAGGNISHHMAMRAGKEKLKP 239

Query: 187 ---------------------------------------IFPTSA-GLEDPRLN-PKSNP 246
                                                  + P S  G +DP  N   S  
Sbjct: 240 RIKGTVIVHPAIWGKDPVDEHDVQDREIRDGVAEVWEKIVSPNSVDGADDPWFNVVGSGS 299

Query: 247 KLGKFRCSGVLICAAEIDPLKYGAVGVHAALKESGFKGSVEMMVSEGEDHGFHLFIPNTE 257
                 C  VL+  A  D      +   A LK+SG+KG VE++  E E+H FHL  P++E
Sbjct: 300 NFSGMGCDKVLVEVAGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEEHCFHLLNPSSE 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900484.11.1e-9763.73probable carboxylesterase 2 [Benincasa hispida] >XP_038900485.1 probable carboxy... [more]
XP_023532126.15.5e-9462.58probable carboxylesterase 2 [Cucurbita pepo subsp. pepo][more]
XP_023007510.11.0e-9261.92probable carboxylesterase 2 [Cucurbita maxima][more]
KAG6605131.11.8e-9262.46putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022948164.12.3e-9262.46probable carboxylesterase 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FX942.3e-5037.34Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1[more]
Q5NUF45.3e-4736.282-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Q9SX789.0e-4735.64Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9SMN02.6e-4635.31Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9LMA74.5e-4635.50Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L3615.0e-9361.92probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=... [more]
A0A6J1G9291.1e-9262.46probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 S... [more]
A0A6J1D5P62.5e-9262.85probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 ... [more]
A0A6P5TIN33.4e-7350.00probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1[more]
A0A314Y2B22.9e-7249.30Putative carboxylesterase 5 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn... [more]
Match NameE-valueIdentityDescription
AT1G49660.11.6e-5137.34carboxyesterase 5 [more]
AT1G47480.16.4e-4835.64alpha/beta-Hydrolases superfamily protein [more]
AT3G48690.11.9e-4735.31alpha/beta-Hydrolases superfamily protein [more]
AT1G19190.13.2e-4735.50alpha/beta-Hydrolases superfamily protein [more]
AT1G49650.15.4e-4737.38alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 16..171
e-value: 4.3E-43
score: 149.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 172..264
e-value: 1.4E-9
score: 39.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 15..253
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 75..171
e-value: 1.9E-29
score: 103.0
NoneNo IPR availablePANTHERPTHR23024:SF414CARBOXYLESTERASE 13-RELATEDcoord: 171..257
coord: 5..172
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 5..172
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 171..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000383.1HG10000383.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity