HG10000346 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000346
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmalonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like
LocationChr09: 4031251 .. 4032609 (-)
RNA-Seq ExpressionHG10000346
SyntenyHG10000346
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTTTTGGAAGTCTGCAAAATTGCTCCCTCACCTTCTCCTTCCACTGATTTCTCTCTTCCTCTCACTTTCTTCGATCTCATGTTCTTCCTAAGCCCTCCCACAGAGCGCATTCTCTTTTACTCCTTCCCCCAAAATGAACATCTTCTTCTTCAATTCGATTCAGTACTCCAAAATCTCAAACATTCTCTCTCTCTTACACTCTCCCATTTCCTCCCTCTCGCTGGAAATCTCGTTTGGCCAAATCACTCTCCACACCCTTTTATTCTTTACAAACCTGGCGACTCTGTTTCTCTCACCATTGCCAAAACTGATGCTGATTTCAACCATCTCTCTTCAAGTCATCTCAAAAAAGCAAACCAATCTCATTTTCTCGTCCCACATCTCCCAATATCCCATACAATTTCTCCAGCCATGTCTCTTCAAATCACTCTGTTTCCCAATTCTGGATTCTCCATTGGCATTATAACCAACCATGTCATTGCTGATGGCAAAACTTCCACCATGTTCTTGAAATCATGGGCTTCCATTTCTAAAACTAACAATAATCATCATCTCCCTCCTCCAATTTTTGATAGGACGGCTGCTATCAATGATCCAATTAACGGGTTGCAATCACTTTACAATAAGTATTTCAAAACATTCATTTCAAATCCCAAAAGCCGCCTTACATCACTTACCTCAAAAATGATAATCTCTGACGATGTGGTTAGTGGCACGTTTGAGCTTACACGTGCTGACATAGAGCATCTAAGGAGAAAAGTGAATGAAGTTTCTTCATCGATTCGTCATTTAACAACGTTTGTATTAACATTCGCTTATGTCTCGACATGTCTTGTAAAATCACAGGGAGTTGAAGGAGAAAGTAAGATTGCTTTGTCGTTCTTGGTGGATTGGCGGTCTCGTGTGGGAACTTTCGGAGGGGTTAATTATTTTGGTAATGGGGTAAGTGGGTATGCGGTGTTTGTTAAAGGAAGAGAATTTTTGGAAGAAAATGCTGTGGTAATGGTTGCGAAGAAGATAAGTGAGGGAATAACAGAGATAACAGAGAATGTGGAGAATGAGAAGAGTTTAGTGGAAATGTTTGAAGAAAATGCAATGCAATGGAGAAAGGAGATGCCCATAGCTAAAACCATTGTAGTGGGAGGTTCGCCAAGATTTGGAGTTTATGAGATTGATTTTGGGTGGGGAAGTTGTAAGAAAGTGGAGATGGTTTCGATAAACCCAAGTGGAAGTTTTTCAATGGCGGATAATAAGAATGGGAATGGAGGAGTGGAACTTGGAATTGCTCTTTCGGAGAATCTTATGAATGTTTTCTCTTCTCTGTTTTTTGATGGACTGAAAGAAAAGTGTTAA

mRNA sequence

ATGGAGGTTTTGGAAGTCTGCAAAATTGCTCCCTCACCTTCTCCTTCCACTGATTTCTCTCTTCCTCTCACTTTCTTCGATCTCATGTTCTTCCTAAGCCCTCCCACAGAGCGCATTCTCTTTTACTCCTTCCCCCAAAATGAACATCTTCTTCTTCAATTCGATTCAGTACTCCAAAATCTCAAACATTCTCTCTCTCTTACACTCTCCCATTTCCTCCCTCTCGCTGGAAATCTCGTTTGGCCAAATCACTCTCCACACCCTTTTATTCTTTACAAACCTGGCGACTCTGTTTCTCTCACCATTGCCAAAACTGATGCTGATTTCAACCATCTCTCTTCAAGTCATCTCAAAAAAGCAAACCAATCTCATTTTCTCGTCCCACATCTCCCAATATCCCATACAATTTCTCCAGCCATGTCTCTTCAAATCACTCTGTTTCCCAATTCTGGATTCTCCATTGGCATTATAACCAACCATGTCATTGCTGATGGCAAAACTTCCACCATGTTCTTGAAATCATGGGCTTCCATTTCTAAAACTAACAATAATCATCATCTCCCTCCTCCAATTTTTGATAGGACGGCTGCTATCAATGATCCAATTAACGGGTTGCAATCACTTTACAATAAGTATTTCAAAACATTCATTTCAAATCCCAAAAGCCGCCTTACATCACTTACCTCAAAAATGATAATCTCTGACGATGTGGTTAGTGGCACGTTTGAGCTTACACGTGCTGACATAGAGCATCTAAGGAGAAAAGTGAATGAAGTTTCTTCATCGATTCGTCATTTAACAACGTTTGTATTAACATTCGCTTATGTCTCGACATGTCTTGTAAAATCACAGGGAGTTGAAGGAGAAAGTAAGATTGCTTTGTCGTTCTTGGTGGATTGGCGGTCTCGTGTGGGAACTTTCGGAGGGGTTAATTATTTTGGTAATGGGGTAAGTGGGTATGCGGTGTTTGTTAAAGGAAGAGAATTTTTGGAAGAAAATGCTGTGGTAATGGTTGCGAAGAAGATAAGTGAGGGAATAACAGAGATAACAGAGAATGTGGAGAATGAGAAGAGTTTAGTGGAAATGTTTGAAGAAAATGCAATGCAATGGAGAAAGGAGATGCCCATAGCTAAAACCATTGTAGTGGGAGGTTCGCCAAGATTTGGAGTTTATGAGATTGATTTTGGGTGGGGAAGTTGTAAGAAAGTGGAGATGGTTTCGATAAACCCAAGTGGAAGTTTTTCAATGGCGGATAATAAGAATGGGAATGGAGGAGTGGAACTTGGAATTGCTCTTTCGGAGAATCTTATGAATGTTTTCTCTTCTCTGTTTTTTGATGGACTGAAAGAAAAGTGTTAA

Coding sequence (CDS)

ATGGAGGTTTTGGAAGTCTGCAAAATTGCTCCCTCACCTTCTCCTTCCACTGATTTCTCTCTTCCTCTCACTTTCTTCGATCTCATGTTCTTCCTAAGCCCTCCCACAGAGCGCATTCTCTTTTACTCCTTCCCCCAAAATGAACATCTTCTTCTTCAATTCGATTCAGTACTCCAAAATCTCAAACATTCTCTCTCTCTTACACTCTCCCATTTCCTCCCTCTCGCTGGAAATCTCGTTTGGCCAAATCACTCTCCACACCCTTTTATTCTTTACAAACCTGGCGACTCTGTTTCTCTCACCATTGCCAAAACTGATGCTGATTTCAACCATCTCTCTTCAAGTCATCTCAAAAAAGCAAACCAATCTCATTTTCTCGTCCCACATCTCCCAATATCCCATACAATTTCTCCAGCCATGTCTCTTCAAATCACTCTGTTTCCCAATTCTGGATTCTCCATTGGCATTATAACCAACCATGTCATTGCTGATGGCAAAACTTCCACCATGTTCTTGAAATCATGGGCTTCCATTTCTAAAACTAACAATAATCATCATCTCCCTCCTCCAATTTTTGATAGGACGGCTGCTATCAATGATCCAATTAACGGGTTGCAATCACTTTACAATAAGTATTTCAAAACATTCATTTCAAATCCCAAAAGCCGCCTTACATCACTTACCTCAAAAATGATAATCTCTGACGATGTGGTTAGTGGCACGTTTGAGCTTACACGTGCTGACATAGAGCATCTAAGGAGAAAAGTGAATGAAGTTTCTTCATCGATTCGTCATTTAACAACGTTTGTATTAACATTCGCTTATGTCTCGACATGTCTTGTAAAATCACAGGGAGTTGAAGGAGAAAGTAAGATTGCTTTGTCGTTCTTGGTGGATTGGCGGTCTCGTGTGGGAACTTTCGGAGGGGTTAATTATTTTGGTAATGGGGTAAGTGGGTATGCGGTGTTTGTTAAAGGAAGAGAATTTTTGGAAGAAAATGCTGTGGTAATGGTTGCGAAGAAGATAAGTGAGGGAATAACAGAGATAACAGAGAATGTGGAGAATGAGAAGAGTTTAGTGGAAATGTTTGAAGAAAATGCAATGCAATGGAGAAAGGAGATGCCCATAGCTAAAACCATTGTAGTGGGAGGTTCGCCAAGATTTGGAGTTTATGAGATTGATTTTGGGTGGGGAAGTTGTAAGAAAGTGGAGATGGTTTCGATAAACCCAAGTGGAAGTTTTTCAATGGCGGATAATAAGAATGGGAATGGAGGAGTGGAACTTGGAATTGCTCTTTCGGAGAATCTTATGAATGTTTTCTCTTCTCTGTTTTTTGATGGACTGAAAGAAAAGTGTTAA

Protein sequence

MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISKTNNNHHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMIISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKIALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKEKC
Homology
BLAST of HG10000346 vs. NCBI nr
Match: XP_038901948.1 (phenolic glucoside malonyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 753.1 bits (1943), Expect = 1.4e-213
Identity = 382/449 (85.08%), Postives = 412/449 (91.76%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVCKI+PS SP +DF LPLT FDLMFFLSPPTERILFYS P N H LL FDS LQ 
Sbjct: 10  MEVLEVCKISPSQSPPSDFCLPLTLFDLMFFLSPPTERILFYS-PSNHH-LLPFDSFLQI 69

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLT+AKTDADFNHLSS+ LK A
Sbjct: 70  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTVAKTDADFNHLSSNQLKIA 129

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
           NQSHFLVP+LP S +++PAMSLQITLFPNSGFSIGI TNHV+ADG+TST+FLKSWASISK
Sbjct: 130 NQSHFLVPYLPKSQSMAPAMSLQITLFPNSGFSIGITTNHVVADGRTSTLFLKSWASISK 189

Query: 181 TNNNHHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMIISDDVVSG 240
           TNNN+H P P+FDRTAA NDPINGLQSLYNKYF+TFISNPKSRLTSLTSK+++SDDVVSG
Sbjct: 190 TNNNNHRPTPVFDRTAATNDPINGLQSLYNKYFETFISNPKSRLTSLTSKVVLSDDVVSG 249

Query: 241 TFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKIALSFLVDW 300
           TFEL RAD+++LRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQ +EG SKIALSFLVDW
Sbjct: 250 TFELKRADLDNLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQRIEGGSKIALSFLVDW 309

Query: 301 RSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITENVENEKSLV 360
           R RV T GGVNYFGNGVSGYAV  +GREF EENA+  VAKKISEGITEITE V NEK LV
Sbjct: 310 RPRVETLGGVNYFGNGVSGYAVIFEGREFEEENAMATVAKKISEGITEITE-VVNEKRLV 369

Query: 361 EMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSGSFSMADNK 420
           EM EENAM+WR EMPI KTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSGSFSMA+++
Sbjct: 370 EMLEENAMRWRNEMPIDKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSGSFSMAESR 429

Query: 421 NGNGGVELGIALSENLMNVFSSLFFDGLK 450
           NGNGGVE+GIAL EN+MNVFSSLFFDG+K
Sbjct: 430 NGNGGVEVGIALPENVMNVFSSLFFDGVK 455

BLAST of HG10000346 vs. NCBI nr
Match: XP_022943466.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita moschata])

HSP 1 Score: 574.3 bits (1479), Expect = 9.1e-160
Identity = 304/458 (66.38%), Postives = 361/458 (78.82%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ F+SPPTERILFYS P N+  LL+FDS+LQ 
Sbjct: 9   MEVLEVCNITPSTSPPTHFSLPLTFSDLVQFISPPTERILFYSLPAND--LLRFDSILQT 68

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP+HS  PFILY PGDSVSLTIAKTDADF+HL S+H++KA
Sbjct: 69  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTDADFDHLLSNHVRKA 128

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP SGFSIGIIT+HV+ DGKTSTMFLKSWASI  
Sbjct: 129 KESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASICS 188

Query: 181 T-----NNNHHLPP---PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMI 240
           T     NN   LPP   P FDRTAA  DP NGL+ LY K++  F+  PK  +  LT K +
Sbjct: 189 TLNCTNNNLPTLPPELTPFFDRTAA-KDP-NGLERLYVKHYGAFVRQPK--ILVLTPKRV 248

Query: 241 ISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKI 300
           ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A +STC+VK++ +E +SKI
Sbjct: 249 ISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSKI 308

Query: 301 ALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITEN 360
           AL F VD R R+G  GG+NYFGNGV G   F++ +E  EEN + MVA KIS+ I E+ ++
Sbjct: 309 ALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEKS 368

Query: 361 VENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSG 420
           V+ E  +VEM EE   +W +EMPI+K IV GGSPRFGVYEIDFGWG+ KKVEMVSINP+G
Sbjct: 369 VK-ENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPNG 428

Query: 421 SFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           SFSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 429 SFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVKE 459

BLAST of HG10000346 vs. NCBI nr
Match: KAG7010988.1 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 572.8 bits (1475), Expect = 2.7e-159
Identity = 301/459 (65.58%), Postives = 362/459 (78.87%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ F+SPPTERILFYS P N+  L +FDS+LQ 
Sbjct: 1   MEVLEVCNITPSTSPPTHFSLPLTFSDLVQFMSPPTERILFYSLPAND--LPRFDSILQT 60

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP HSP PFI+Y PGDSVSLTIAKT+ADF+HL S+H++KA
Sbjct: 61  LKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSNHVRKA 120

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+PAMSLQITLFP SGFSIGIIT+HV+ DGKTSTMFLKSWASI  
Sbjct: 121 KESHFLVPQLPTSDTIAPAMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASICS 180

Query: 181 T---NNNHHLP------PPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T    NN++LP       P FDRTAA  DP NGL+ LY K++  F+  PK  +  LT K 
Sbjct: 181 TLNCTNNNNLPTLPPELTPFFDRTAA-KDP-NGLERLYVKHYGAFVRQPK--ILVLTPKR 240

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESK 300
           +ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A +STC+VK++ +E +SK
Sbjct: 241 VISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSK 300

Query: 301 IALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
           IAL F VD R R+G  GG+NYFGNGV G   F++ +E  EEN + MVA KIS+ I E+ +
Sbjct: 301 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEK 360

Query: 361 NVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           +V+ E  +VEM EE   +W +EMPI+K IV GGSPRFGVYEIDFGWG+ KKVEMVSINP+
Sbjct: 361 SVK-ENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPN 420

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           G+FSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 421 GNFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVKE 452

BLAST of HG10000346 vs. NCBI nr
Match: XP_023512233.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.6 bits (1472), Expect = 5.9e-159
Identity = 301/459 (65.58%), Postives = 361/459 (78.65%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ F+SPPTERILFYS P N+  L +FDS+LQ 
Sbjct: 50  MEVLEVCNITPSTSPLTHFSLPLTFSDLVQFISPPTERILFYSLPAND--LPRFDSILQT 109

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP HSP PFI+Y PGDSVSLTIAKT+ADF+HL S+H++KA
Sbjct: 110 LKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSNHVRKA 169

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP SGFSIGIIT+HV+ DGKTSTMFLKSWASI  
Sbjct: 170 KESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASICS 229

Query: 181 T---NNNHHLP------PPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T    NN++LP       P FDRTAA  DP NGL+ LY K++  F+  PK  +  LT K 
Sbjct: 230 TLNCTNNNNLPTLPPELTPFFDRTAA-KDP-NGLERLYVKHYGAFVRQPK--ILVLTPKR 289

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESK 300
           +ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A +STC+VK++ +E +SK
Sbjct: 290 VISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSK 349

Query: 301 IALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
           IAL F VD R R+G  GG+NYFGNGV G   F++ +E  EEN + MVA KIS+ I E+ +
Sbjct: 350 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEK 409

Query: 361 NVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           +V+ E  +VEM EE   +W +EMPI+K IV GGSPRFGVYEIDFGWG+ KKVEMVSINP+
Sbjct: 410 SVK-ENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPN 469

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           GSFSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 470 GSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVKE 501

BLAST of HG10000346 vs. NCBI nr
Match: XP_023522403.1 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.6 bits (1472), Expect = 5.9e-159
Identity = 301/459 (65.58%), Postives = 361/459 (78.65%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ F+SPPTERILFYS P N+  L +FDS+LQ 
Sbjct: 6   MEVLEVCNITPSTSPLTHFSLPLTFSDLVQFISPPTERILFYSLPAND--LPRFDSILQT 65

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP HSP PFI+Y PGDSVSLTIAKT+ADF+HL S+H++KA
Sbjct: 66  LKHSLSLTLSHFLPLAGNLVWPQHSPEPFIVYNPGDSVSLTIAKTEADFDHLLSNHVRKA 125

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP SGFSIGIIT+HV+ DGKTSTMFLKSWASI  
Sbjct: 126 KESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASICS 185

Query: 181 T---NNNHHLP------PPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T    NN++LP       P FDRTAA  DP NGL+ LY K++  F+  PK  +  LT K 
Sbjct: 186 TLNCTNNNNLPTLPPELTPFFDRTAA-KDP-NGLERLYVKHYGAFVRQPK--ILVLTPKR 245

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESK 300
           +ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A +STC+VK++ +E +SK
Sbjct: 246 VISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSK 305

Query: 301 IALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
           IAL F VD R R+G  GG+NYFGNGV G   F++ +E  EEN + MVA KIS+ I E+ +
Sbjct: 306 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEK 365

Query: 361 NVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           +V+ E  +VEM EE   +W +EMPI+K IV GGSPRFGVYEIDFGWG+ KKVEMVSINP+
Sbjct: 366 SVK-ENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPN 425

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           GSFSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 426 GSFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVKE 457

BLAST of HG10000346 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 7.4e-72
Identity = 177/471 (37.58%), Postives = 267/471 (56.69%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTD-FSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVL- 60
           ++V++V ++ PS S S++  +LPLTFFDL+++     ER++FY           FDSV+ 
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPF--FDSVIV 69

Query: 61  QNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLK 120
            NLK SLS +LSH+LPLAG LVW    P P I+Y P D+VS T+A+++ADF+ L+     
Sbjct: 70  PNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPF 129

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
              + + LVP L +S   + A+S Q+TLFPN GF I +  +H + DGKT+T FLKSWA  
Sbjct: 130 PTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWART 189

Query: 181 SKTNNNHHLPP---PIFDRTAAINDPIN---GLQSLYNKYFKTFI-----SNPKSRLTSL 240
            K N +  LP    P++DRT  I DP++    + + +++  K F       NPKS    L
Sbjct: 190 CK-NQDSFLPQDLIPVYDRT-VIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKS--LKL 249

Query: 241 TSKMIISDDVVSGTFELTRADIEHLRRKVNEVSSSIR--------HLTTFVLTFAYVSTC 300
                I  DV   T  LTR DI+ LR ++ + SSS           L+TFV+ ++Y  TC
Sbjct: 250 LWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTC 309

Query: 301 LVKSQGVEGESKIALSFLVDWRSRVGTFGGVNYFGNGVSG-YAVFVKGREFLEENAVVMV 360
           L+K++G +    +   F VD RS +      +YFGN VS  + + +    F+ E   +  
Sbjct: 310 LIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAA 369

Query: 361 AKKISEGITEITENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWG 420
           A+ +S+ +  + ENV     + E+ E     +    P  + + V GS RFGVY +DFGWG
Sbjct: 370 ARMVSDSVEALDENV--ALKIPEILE----GFTTLSPGTQVLSVAGSTRFGVYGLDFGWG 429

Query: 421 SCKKVEMVSINPSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
             +KV +VSI+   + S A++++G+GGVELG +L ++ M+V   L   GL+
Sbjct: 430 RPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of HG10000346 vs. ExPASy Swiss-Prot
Match: Q9LJB4 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis thaliana OX=3702 GN=5MAT PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.8e-70
Identity = 174/460 (37.83%), Postives = 262/460 (56.96%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           + +LEV ++  SP  S   +LPLT+FDL +    P +R+LFY  P+     L   S++  
Sbjct: 8   VNILEVVQV--SPPSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPE-----LTRSSLISK 67

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPG--DSVSLTIAKTDADFNHLSSSHLK 120
           LK SLS TL H+LPLAG LVW +    P I+Y P   D+V LT+A+++ D +HLS    +
Sbjct: 68  LKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPR 127

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
            A + H LVP LP+S   +  +++Q+T FPN GFS+G+  +H + DGKT+ MFLK+WA  
Sbjct: 128 PATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHN 187

Query: 181 SKTNNN---HHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMIISD 240
            K       H L P + DR   + DP      L N++     SN K  L    SK II  
Sbjct: 188 CKQEQEALPHDLVPSL-DR-IIVQDPTGLETKLLNRWISA--SNNKPSLKLFPSK-IIGS 247

Query: 241 DVVSGTFELTRADIEHLRRKVNEVSSSIR-HLTTFVLTFAYVSTCLVKSQGVEGESKIAL 300
           D++  T+ LTR DI+ LR +V   S + +  L+TFV+T+AYV TC+VK +G +    + +
Sbjct: 248 DILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCV 307

Query: 301 SFLVDWRSRVGTFGGVNYFGNGVSGYAVF-VKGREFLEE---NAVVMVAKKISEGITEI- 360
            F  D+RSR+       +FGN + G   F VK    LEE      +   + ++  +  + 
Sbjct: 308 GFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLC 367

Query: 361 TENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSIN 420
            EN+  EK+++  FE     +++  P  + I V GS R G+Y  DFGWG   KVE+V+I+
Sbjct: 368 PENI--EKNMLLPFE----AFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTID 427

Query: 421 PSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
              S S++++ +G+GGVE+G+ L ++ +  F SLF  GL+
Sbjct: 428 KDASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE 449

BLAST of HG10000346 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.8e-70
Identity = 171/454 (37.67%), Postives = 259/454 (57.05%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           + V+++ +++   +      LPLTFFDL++    P ER+ FY   ++      F S+L  
Sbjct: 3   LNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSF-FSSILPK 62

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           L+ SLSL LSHFLPL+G+L W    P P I+  P D+VSLT+ +++ADF+++SS  L+  
Sbjct: 63  LEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLE 122

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +   LVP L +S   +  +SLQITLFPN GFSIG   +HV+ DGKT++ F KSWA I K
Sbjct: 123 TELRPLVPELQVSSDSASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICK 182

Query: 181 --TNNNHHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPK--SRLTSLTSKMIISDD 240
             T       P + DRT  IN P    Q ++     ++IS  K  +R  +L     I +D
Sbjct: 183 HGTTPQDFDLPTVLDRT-VINVPAGLEQKIFQ--LSSYISEEKDYARTLTLPPAKEIDND 242

Query: 241 VVSGTFELTRADIEHLR-RKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKIALS 300
           VV  T ELT  DIE L+ R  NE + S  HL+TFV+++AYV TC+VKS G +    +   
Sbjct: 243 VVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRPVRFM 302

Query: 301 FLVDWRSRVGTFGGVNYFGNGV-----SGYAVFVKGREFLEENAVVMVAKKISEGITEIT 360
           +  D+R+R+     + YFGN V     +GY    K   FL ++  V   + +S+ +  + 
Sbjct: 303 YAADFRNRLDPPVPLTYFGNCVLPIDFNGY----KATTFLGKDGYVNGVEILSDSVRGL- 362

Query: 361 ENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINP 420
               N +S+ E++E+     + +    + + V GS +FG+Y  DFGWG   K +++S+  
Sbjct: 363 -GSRNIESIWEVYEDGTKNMKLD---TQNVTVTGSNQFGIYGSDFGWGRPVKTDVMSLYK 422

Query: 421 SGSFSMADNKNGNGGVELGIALSENLMNVFSSLF 445
           +  FSM+  ++  GG+E+GI+L +  MNVF SLF
Sbjct: 423 NNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLF 443

BLAST of HG10000346 vs. ExPASy Swiss-Prot
Match: Q9LRQ8 (Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.9e-67
Identity = 174/467 (37.26%), Postives = 260/467 (55.67%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFS---------LPLTFFDLMFFLSPPTERILFYSFPQN--EH 60
           + V+E  ++ P     TD+S         LPLTFFDL + L  P +R+ FY   ++  +H
Sbjct: 3   LHVIETARVTP-----TDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDH 62

Query: 61  LLLQFDSVLQNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADF 120
                  +L  LK SLSL L ++LPL G++ W  + P P I+      V +TIA++DADF
Sbjct: 63  F---HSIILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADF 122

Query: 121 NHLSSSHLKKANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTST 180
           +HLS    +  ++ H LVP LP+S   + A S+QITLFPN GFSIG+  +H + DGKTS+
Sbjct: 123 SHLSGYGQRPLSELHALVPKLPVSDDSATAFSIQITLFPNQGFSIGVAAHHAVLDGKTSS 182

Query: 181 MFLKSWASISKTNNNHHLP---PPIFDRTAAINDPINGLQSLYN--KYFKTFISNPKSRL 240
            F+K+WA I K      +P    P +DR + I  P    + +    +  K   +N +S L
Sbjct: 183 TFIKAWAQICK-QELQSMPENLTPSYDR-SLIKYPTYLDEKMIELVRSLKEDQTNIRS-L 242

Query: 241 TSLTSKMIISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQ 300
           TSL S   + DDVV  T  L+RADIE LR +V  VS S+ HL+TFV+ +AY  TC VK++
Sbjct: 243 TSLPSSK-LGDDVVLATLVLSRADIERLREQVKNVSPSL-HLSTFVIAYAYAWTCFVKAR 302

Query: 301 GVEGESKIALSFLVDWRSRVGTFGGVNYFGNGVSGYAVF-VKGREFLEENAVVMVAKKIS 360
           G   +  ++L F+ D+R R+       YFGN +     +  K  EF+EE   V  A+ IS
Sbjct: 303 GGNKDRSVSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEIIS 362

Query: 361 EGITEITENVENEKSLVEMFEE--NAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCK 420
           + +  ++      +++ + F E  +   W  +        + GS R GVYE DFGWG   
Sbjct: 363 DLVKGLSS--RKIETIADTFVEGFSFQSWSTQFG-----TIAGSTRLGVYEADFGWGRPV 422

Query: 421 KVEMVSINPSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGL 449
           KV++VSI+   + +MA+ ++ +GGVE+G+ L +  M+   S F +GL
Sbjct: 423 KVDIVSIDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGL 449

BLAST of HG10000346 vs. ExPASy Swiss-Prot
Match: Q9FNP9 (Agmatine coumaroyltransferase OS=Arabidopsis thaliana OX=3702 GN=ACT PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 5.3e-62
Identity = 148/454 (32.60%), Postives = 250/454 (55.07%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLL-LQFDSVLQ 60
           ++V+++ +++P+ +      +PL+FFDL +    PTE++ FY   ++     + + S+L 
Sbjct: 3   LKVIKISRVSPATASVDPLIVPLSFFDLQWLKLNPTEQVFFYKLTESSSSRDVFYSSILP 62

Query: 61  NLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKK 120
            L+ SLSL L+HF    G+L W +  P P ++   GD++SLT+A+TDADF+ +S   L+ 
Sbjct: 63  KLERSLSLILTHFRLFTGHLKWDSQDPKPHLVVLSGDTLSLTVAETDADFSRISGRGLRP 122

Query: 121 ANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASIS 180
             +   L+P LPI       +SLQ+TLFP  GF IG   +HV+ DGKT+  F K+WA   
Sbjct: 123 ELELRPLIPELPIYSDSGAVVSLQVTLFPKQGFCIGTTAHHVVLDGKTAEKFNKAWAHTC 182

Query: 181 KTNNNHHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSL----TSKMIISD 240
           K      + P + DR + +N P  GL+    +       + K    +L      ++   D
Sbjct: 183 KHGTIPKILPTVLDR-SVVNVPA-GLEQKMLELLPYLTEDDKENGRTLKLPPVKEINAKD 242

Query: 241 DVVSGTFELTRADIEHLRRKVNEVSSSIR-HLTTFVLTFAYVSTCLVKSQGVEGESKIAL 300
           +V+  T E++  +IE L+ +  + S+    HL+TFV+TFA+V TC+VK++  +    +  
Sbjct: 243 NVLRITIEISPENIEKLKERAKKESTRAELHLSTFVVTFAHVWTCMVKARSGDPNRPVRF 302

Query: 301 SFLVDWRSRVGTFGGVNYFGNGVSGYAVF-VKGREFLEENAVVMVAKKISEGITEITENV 360
            +  D+R+R+     V YFG  V     +  K +EF+ E+  V   + +S+ +  +    
Sbjct: 303 MYAADFRNRLEPPVPVTYFGTCVLAMDFYKYKAKEFMGEDGFVNTVEILSDSVKRLAS-- 362

Query: 361 ENEKSLVEMFEE--NAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           +  +S  +++EE    M+W  ++     +VV GS + G+YE DFGWG     E +SI  +
Sbjct: 363 QGVESTWKVYEEGTKTMKWGTQL-----LVVNGSNQIGMYETDFGWGRPIHTETMSIYKN 422

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFF 446
             FSM+  ++G GGVE+GI+L +  M+ F SLF+
Sbjct: 423 DEFSMSKRRDGIGGVEIGISLKKLEMDTFLSLFY 447

BLAST of HG10000346 vs. ExPASy TrEMBL
Match: A0A6J1FRS6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448225 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 4.4e-160
Identity = 304/458 (66.38%), Postives = 361/458 (78.82%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ F+SPPTERILFYS P N+  LL+FDS+LQ 
Sbjct: 9   MEVLEVCNITPSTSPPTHFSLPLTFSDLVQFISPPTERILFYSLPAND--LLRFDSILQT 68

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP+HS  PFILY PGDSVSLTIAKTDADF+HL S+H++KA
Sbjct: 69  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTDADFDHLLSNHVRKA 128

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP SGFSIGIIT+HV+ DGKTSTMFLKSWASI  
Sbjct: 129 KESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIITSHVVVDGKTSTMFLKSWASICS 188

Query: 181 T-----NNNHHLPP---PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMI 240
           T     NN   LPP   P FDRTAA  DP NGL+ LY K++  F+  PK  +  LT K +
Sbjct: 189 TLNCTNNNLPTLPPELTPFFDRTAA-KDP-NGLERLYVKHYGAFVRQPK--ILVLTPKRV 248

Query: 241 ISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKI 300
           ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A +STC+VK++ +E +SKI
Sbjct: 249 ISDDVVHATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALISTCIVKAERIEPKSKI 308

Query: 301 ALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITEN 360
           AL F VD R R+G  GG+NYFGNGV G   F++ +E  EEN + MVA KIS+ I E+ ++
Sbjct: 309 ALFFAVDMRPRMGALGGLNYFGNGVIGLGEFLEAKELEEENGMAMVANKISDRIMEMEKS 368

Query: 361 VENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPSG 420
           V+ E  +VEM EE   +W +EMPI+K IV GGSPRFGVYEIDFGWG+ KKVEMVSINP+G
Sbjct: 369 VK-ENKVVEMVEEILGRWGREMPISKMIVAGGSPRFGVYEIDFGWGNSKKVEMVSINPNG 428

Query: 421 SFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           SFSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 429 SFSMTESRSGNGGVEVGVILPKDVMNVFSSLFYEEVKE 459

BLAST of HG10000346 vs. ExPASy TrEMBL
Match: A0A6J1J6U6 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111483993 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 2.0e-157
Identity = 299/459 (65.14%), Postives = 360/459 (78.43%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PS SP T FSLPLTF DL+ FLSPPTERILFY+   N   L +FDS+LQ 
Sbjct: 10  MEVLEVCNITPSTSPPTHFSLPLTFSDLVQFLSPPTERILFYTLHANH--LPRFDSILQT 69

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP+HSP PFILY  GDSVSLTIAKT+ADF+HLSS+H++KA
Sbjct: 70  LKHSLSLTLSHFLPLAGNLVWPHHSPDPFILYNHGDSVSLTIAKTEADFHHLSSNHVRKA 129

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+PAMSLQITLFP SGFSIGIITNHV+ DGKTSTMFLKSWASI  
Sbjct: 130 TESHFLVPQLPTSDTIAPAMSLQITLFPKSGFSIGIITNHVVVDGKTSTMFLKSWASICS 189

Query: 181 T---NNNHHLP------PPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T    NN++LP       P FDRTAA  DP NGLQ LY K++  F+  PK  +  LT K 
Sbjct: 190 TLNCTNNNNLPTLPSELTPFFDRTAA-KDP-NGLQRLYVKHYGAFVRQPK--ILGLTPKR 249

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESK 300
           +ISDDVV  TFELTR DIE LRRKV   S+S RHLTTFVLT+A VSTC+V+++ +E +SK
Sbjct: 250 VISDDVVYATFELTRIDIEKLRRKVATNSTSTRHLTTFVLTYALVSTCIVQAERIEPKSK 309

Query: 301 IALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
           IAL F VD R R+G  GG+NYFGNGV G    ++ +E  EEN + MVA KIS+ I E+ +
Sbjct: 310 IALFFAVDMRPRMGALGGLNYFGNGVIGLGEILEAKELEEENGMAMVANKISDRIMEMEK 369

Query: 361 NVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           +V+ E  +VEM EE + +W +EMP++K IV GGSPRFGVYEIDFGWG+ KKVEM S++P+
Sbjct: 370 SVK-ENKVVEMVEEISDRWGREMPVSKMIVAGGSPRFGVYEIDFGWGNSKKVEMASMDPN 429

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           GSFSM ++++GNGGVE+G+ L +++MNVFSSLF++ +KE
Sbjct: 430 GSFSMTESRSGNGGVEVGVVLPKDVMNVFSSLFYEEVKE 461

BLAST of HG10000346 vs. ExPASy TrEMBL
Match: A0A6J1FXM3 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448598 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 3.3e-155
Identity = 298/459 (64.92%), Postives = 356/459 (77.56%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           MEVLEVC I PSPSP T FSLPLTFFDL+ FL+PPTERILFY+ P N+  L +FDS+LQ 
Sbjct: 22  MEVLEVCNITPSPSPPTHFSLPLTFFDLVQFLAPPTERILFYTLPAND--LPRFDSILQT 81

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLSLTLSHFLPLAGNLVWP+HS  PFILY PGDSVSLTIAKT+ADF+HLSS+H++KA
Sbjct: 82  LKHSLSLTLSHFLPLAGNLVWPHHSQDPFILYNPGDSVSLTIAKTEADFHHLSSNHVRKA 141

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP SGFSIGI+TNHV+ DGKTSTMFLKSWASI  
Sbjct: 142 KESHFLVPQLPTSDTIAPPMSLQITLFPKSGFSIGIVTNHVVVDGKTSTMFLKSWASICS 201

Query: 181 T-----NNNHHLPP---PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMI 240
           T     NN   LPP   P FDRTAA  DP NGLQ LY KY+  F+  PK  +  LT K +
Sbjct: 202 TLNCTNNNLPTLPPELTPFFDRTAA-RDP-NGLQRLYVKYYGAFVRQPK--ILGLTPKRV 261

Query: 241 ISDDVVSGTFELTRADIEHLRRKVNEVSSSI-RHLTTFVLTFAYVSTCLVKSQGVEGESK 300
           ISDDVV  TFELTR DIE LR KV   SSS  R L+TF+L F+ +STC+VK++ +E +SK
Sbjct: 262 ISDDVVYATFELTRTDIEKLRTKVVATSSSTPRRLSTFMLAFSLISTCIVKAERIEPKSK 321

Query: 301 IALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
           IAL F +D R R+G  GG+NYFGN VS   VF++ +E  EEN + MVA KIS+ + EI E
Sbjct: 322 IALFFAMDMRPRMGALGGLNYFGNCVSTLGVFLEAKELEEENGMAMVANKISDRMMEI-E 381

Query: 361 NVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINPS 420
           N   E  +VEM EE   +W +EMPI+K I VGGSPRFGVYEIDFGWG+ KKVEM S++P+
Sbjct: 382 NSVKENKVVEMVEEILGRWGREMPISKMIGVGGSPRFGVYEIDFGWGNSKKVEMASMDPN 441

Query: 421 GSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLKE 451
           G+FS+ ++++GNGGVE+GIAL  ++MNV SSLF++ +KE
Sbjct: 442 GTFSITESRSGNGGVEVGIALPNDVMNVLSSLFYEEVKE 473

BLAST of HG10000346 vs. ExPASy TrEMBL
Match: A0A6J1FVL4 (malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111448866 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 1.3e-148
Identity = 286/459 (62.31%), Postives = 343/459 (74.73%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           +E+LE CKI PSPSP T FSLPLT+FDL FFLSPPT+RILFYS P +E  LL F S+LQ 
Sbjct: 14  LEILEQCKIGPSPSPPTPFSLPLTYFDLSFFLSPPTQRILFYSLPPHE--LLHFYSILQT 73

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLS  LSHFLPLAGNLVWP  SP PFI Y PGDS+SLTIAKT ADF+HLSS+H +KA
Sbjct: 74  LKHSLSHALSHFLPLAGNLVWPPQSPEPFICYNPGDSLSLTIAKTHADFHHLSSNHARKA 133

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+P MSLQITLFP  GFSIGIITNHV++D KTSTMFLKSWASI  
Sbjct: 134 KESHFLVPDLPTSDTIAPVMSLQITLFPKCGFSIGIITNHVVSDAKTSTMFLKSWASICS 193

Query: 181 T--NNNHHLPP-------PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T  N N+  PP       P FDRT A  DP NGL +LY K F  F+ NPK  L  LT K 
Sbjct: 194 TLNNTNNKNPPTLPSELTPCFDRTPA-TDP-NGLHTLYVKSFAIFVPNPK--LLGLTPKE 253

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSI-RHLTTFVLTFAYVSTCLVKSQGVEGES 300
           +ISDDVV  TFELTR DIE LRR+V   SSS  R L+TF+L F+ VSTC+VK+Q  E ES
Sbjct: 254 VISDDVVYATFELTRIDIEKLRRRVVATSSSTPRRLSTFMLAFSLVSTCIVKAQRTEPES 313

Query: 301 KIALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEIT 360
           KI L FLVDWR+R+   G +NYFGNGV+GY +FV+ RE   EN + M++ KIS+ + EI 
Sbjct: 314 KIGLIFLVDWRARMDVLGWLNYFGNGVNGYGLFVEARELEGENGMAMISNKISDALAEIE 373

Query: 361 ENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINP 420
           +N++  K  VEM +E + +W+K MPI K ++V GSPR GVY+IDFGWG  KKVE+ SI+P
Sbjct: 374 KNMKENKG-VEMLDEISERWKKAMPIDKFVLVAGSPRLGVYDIDFGWGRSKKVELASISP 433

Query: 421 SGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
           +G FSMA+++NG+GGVELGI+L    ++ F SLF + +K
Sbjct: 434 NGVFSMAESRNGDGGVELGISLPSEAVDKFCSLFSETMK 465

BLAST of HG10000346 vs. ExPASy TrEMBL
Match: A0A6J1JFR0 (phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483994 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.7e-143
Identity = 284/459 (61.87%), Postives = 335/459 (72.98%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           ME+LEVCKI+PSPSP T FSLPLT FDL FF +PPT+ ILFYS   + H LL  DS+L N
Sbjct: 3   MEILEVCKISPSPSPPTPFSLPLTLFDLSFFSAPPTQHILFYSL--SPHQLLHLDSILLN 62

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           LKHSLS  LSHFLPLAG+LVWP  SP PFILY PGDSVSLTIAKT ADF+ LSS+H +KA
Sbjct: 63  LKHSLSHALSHFLPLAGSLVWPPQSPDPFILYNPGDSVSLTIAKTHADFHLLSSNHARKA 122

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +SHFLVP LP S TI+PAMSLQITLFP SGF IGIITNHV++D KTSTMFLKSWASI  
Sbjct: 123 TESHFLVPQLPTSDTIAPAMSLQITLFPKSGFCIGIITNHVVSDAKTSTMFLKSWASICS 182

Query: 181 T--NNNHHLPP-------PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKM 240
           T  N N+  PP       P FDRT+A  DP NGL ++Y K F+ F+     +L  L  K 
Sbjct: 183 TLNNTNNKNPPTLPSELTPCFDRTSA-TDP-NGLHTIYVKSFEIFV----PKLLGLAPKE 242

Query: 241 IISDDVVSGTFELTRADIEHLRRKVNEVSSSI-RHLTTFVLTFAYVSTCLVKSQGVEGES 300
           +ISDDVV  TFELT  DIE +RR+V   SSS  R LTT +L F+  STC+VK+Q +  E 
Sbjct: 243 VISDDVVYATFELTCIDIEKVRRRVVATSSSTPRRLTTLMLAFSLASTCIVKAQRIAPEC 302

Query: 301 KIALSFLVDWRSRVGTFGGVNYFGNGVSGYAVFVKGREFLEENAVVMVAKKISEGITEIT 360
           KI L FLVDWR+R+   GG+NYFGN VS Y VF + RE  EEN + M++ KISE I EI 
Sbjct: 303 KIGLIFLVDWRARMDMLGGLNYFGNCVSAYGVFAEARELEEENGMAMISNKISEEIEEIE 362

Query: 361 ENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINP 420
           +N   E  +VEM E  + +WRKEMPI K I+V GSPR GVY+IDFGWG  KKVE VSI+P
Sbjct: 363 KN-GKENKIVEMLEAISERWRKEMPIDKLIIVAGSPRLGVYDIDFGWGRSKKVEQVSISP 422

Query: 421 SGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
           +G FSMA+++NG+GGVELGIAL    M+ F SLF + +K
Sbjct: 423 NGVFSMAESRNGDGGVELGIALPPQAMDKFCSLFSETVK 452

BLAST of HG10000346 vs. TAIR 10
Match: AT5G39090.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 282.3 bits (721), Expect = 6.7e-76
Identity = 180/459 (39.22%), Postives = 260/459 (56.64%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPS-PSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVL- 60
           +  + V ++ PS S  S   +LPLTFFDL++      ER++FY        L  FDSV+ 
Sbjct: 5   LNFIHVSRVTPSNSNSSASLTLPLTFFDLLWLKHKAVERVIFYKLTDVNRSL--FDSVIV 64

Query: 61  QNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLK 120
            NLK SLS +LSH+LPLAG+++W  H P P I+Y   D+VS T+A++++DF+ L+     
Sbjct: 65  PNLKSSLSSSLSHYLPLAGHIIWEPHDPKPKIVYTQNDAVSFTVAESNSDFSLLTGKEPF 124

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
            + + H LVP L  S   +  +S Q+TLFPN GF IG+ T+H ++DGKT+T FLKSWA +
Sbjct: 125 SSTELHPLVPELQNSDDSAAVVSFQVTLFPNQGFCIGVTTHHAVSDGKTTTTFLKSWAHL 184

Query: 181 SKTNNNHHLPP---PIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMIISD 240
            K + +  LP    P +DRT      I G   +  K  K + S  K +   L  +  I  
Sbjct: 185 CK-HQDSSLPDDLIPFYDRTV-----IKGPPEIDTKVLKIWHSIHKPKSLKLLPRPEIES 244

Query: 241 DVVSGTFELTRADIEHLRRKVNEVSSSIR--HLTTFVLTFAYVSTCLVKSQGVEGESKIA 300
           DVV  TFELTR +IE LR K+   SSS     L+TFV+TF+YV TCL+ S G +    + 
Sbjct: 245 DVVRYTFELTRENIEKLRDKLKRESSSFSSVRLSTFVITFSYVFTCLIGSGGDDPNRPVG 304

Query: 301 LSFLVDWRSRVGTFG-GVNYFGNGV-SGYAVFVKGREFLEENAVVMVAKKISEGITEITE 360
             F VD R  +      + YFGN V S   + +    FL E   V+ A+ IS+ + E+  
Sbjct: 305 YRFAVDCRRLIDDPPIPLTYFGNCVYSAVKIPLDAGMFLGEQGFVVAARLISDSVEELDS 364

Query: 361 NVE-NEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINP 420
           NV      L+E +E+  +        ++ + V GS RFG+Y +DFGWG   K  +VSI+ 
Sbjct: 365 NVAWKIPELLETYEKAPVD-------SQFVSVAGSTRFGIYGLDFGWGKPFKSLLVSIDQ 424

Query: 421 SGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
            G  S+A++++G+GGVE+G +L +  MNV   L   G+K
Sbjct: 425 RGKISIAESRDGSGGVEIGFSLKKQEMNVLIDLLHKGIK 448

BLAST of HG10000346 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 272.7 bits (696), Expect = 5.3e-73
Identity = 177/471 (37.58%), Postives = 267/471 (56.69%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTD-FSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVL- 60
           ++V++V ++ PS S S++  +LPLTFFDL+++     ER++FY           FDSV+ 
Sbjct: 10  LKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPF--FDSVIV 69

Query: 61  QNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLK 120
            NLK SLS +LSH+LPLAG LVW    P P I+Y P D+VS T+A+++ADF+ L+     
Sbjct: 70  PNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKEPF 129

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
              + + LVP L +S   + A+S Q+TLFPN GF I +  +H + DGKT+T FLKSWA  
Sbjct: 130 PTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWART 189

Query: 181 SKTNNNHHLPP---PIFDRTAAINDPIN---GLQSLYNKYFKTFI-----SNPKSRLTSL 240
            K N +  LP    P++DRT  I DP++    + + +++  K F       NPKS    L
Sbjct: 190 CK-NQDSFLPQDLIPVYDRT-VIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKS--LKL 249

Query: 241 TSKMIISDDVVSGTFELTRADIEHLRRKVNEVSSSIR--------HLTTFVLTFAYVSTC 300
                I  DV   T  LTR DI+ LR ++ + SSS           L+TFV+ ++Y  TC
Sbjct: 250 LWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTC 309

Query: 301 LVKSQGVEGESKIALSFLVDWRSRVGTFGGVNYFGNGVSG-YAVFVKGREFLEENAVVMV 360
           L+K++G +    +   F VD RS +      +YFGN VS  + + +    F+ E   +  
Sbjct: 310 LIKARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAA 369

Query: 361 AKKISEGITEITENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWG 420
           A+ +S+ +  + ENV     + E+ E     +    P  + + V GS RFGVY +DFGWG
Sbjct: 370 ARMVSDSVEALDENV--ALKIPEILE----GFTTLSPGTQVLSVAGSTRFGVYGLDFGWG 429

Query: 421 SCKKVEMVSINPSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
             +KV +VSI+   + S A++++G+GGVELG +L ++ M+V   L   GL+
Sbjct: 430 RPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKGLE 468

BLAST of HG10000346 vs. TAIR 10
Match: AT3G29680.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-71
Identity = 171/454 (37.67%), Postives = 259/454 (57.05%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           + V+++ +++   +      LPLTFFDL++    P ER+ FY   ++      F S+L  
Sbjct: 3   LNVIKISRVSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSF-FSSILPK 62

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLKKA 120
           L+ SLSL LSHFLPL+G+L W    P P I+  P D+VSLT+ +++ADF+++SS  L+  
Sbjct: 63  LEQSLSLVLSHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEADFSYISSKELRLE 122

Query: 121 NQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASISK 180
            +   LVP L +S   +  +SLQITLFPN GFSIG   +HV+ DGKT++ F KSWA I K
Sbjct: 123 TELRPLVPELQVSSDSASLLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICK 182

Query: 181 --TNNNHHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPK--SRLTSLTSKMIISDD 240
             T       P + DRT  IN P    Q ++     ++IS  K  +R  +L     I +D
Sbjct: 183 HGTTPQDFDLPTVLDRT-VINVPAGLEQKIFQ--LSSYISEEKDYARTLTLPPAKEIDND 242

Query: 241 VVSGTFELTRADIEHLR-RKVNEVSSSIRHLTTFVLTFAYVSTCLVKSQGVEGESKIALS 300
           VV  T ELT  DIE L+ R  NE + S  HL+TFV+++AYV TC+VKS G +    +   
Sbjct: 243 VVRVTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGDANRPVRFM 302

Query: 301 FLVDWRSRVGTFGGVNYFGNGV-----SGYAVFVKGREFLEENAVVMVAKKISEGITEIT 360
           +  D+R+R+     + YFGN V     +GY    K   FL ++  V   + +S+ +  + 
Sbjct: 303 YAADFRNRLDPPVPLTYFGNCVLPIDFNGY----KATTFLGKDGYVNGVEILSDSVRGL- 362

Query: 361 ENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSINP 420
               N +S+ E++E+     + +    + + V GS +FG+Y  DFGWG   K +++S+  
Sbjct: 363 -GSRNIESIWEVYEDGTKNMKLD---TQNVTVTGSNQFGIYGSDFGWGRPVKTDVMSLYK 422

Query: 421 SGSFSMADNKNGNGGVELGIALSENLMNVFSSLF 445
           +  FSM+  ++  GG+E+GI+L +  MNVF SLF
Sbjct: 423 NNEFSMSARRDEIGGLEIGISLKKCEMNVFLSLF 443

BLAST of HG10000346 vs. TAIR 10
Match: AT3G29590.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.3e-71
Identity = 174/460 (37.83%), Postives = 262/460 (56.96%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTDFSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVLQN 60
           + +LEV ++  SP  S   +LPLT+FDL +    P +R+LFY  P+     L   S++  
Sbjct: 8   VNILEVVQV--SPPSSNSLTLPLTYFDLGWLKLHPVDRVLFYHVPE-----LTRSSLISK 67

Query: 61  LKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPG--DSVSLTIAKTDADFNHLSSSHLK 120
           LK SLS TL H+LPLAG LVW +    P I+Y P   D+V LT+A+++ D +HLS    +
Sbjct: 68  LKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGDEPR 127

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
            A + H LVP LP+S   +  +++Q+T FPN GFS+G+  +H + DGKT+ MFLK+WA  
Sbjct: 128 PATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAWAHN 187

Query: 181 SKTNNN---HHLPPPIFDRTAAINDPINGLQSLYNKYFKTFISNPKSRLTSLTSKMIISD 240
            K       H L P + DR   + DP      L N++     SN K  L    SK II  
Sbjct: 188 CKQEQEALPHDLVPSL-DR-IIVQDPTGLETKLLNRWISA--SNNKPSLKLFPSK-IIGS 247

Query: 241 DVVSGTFELTRADIEHLRRKVNEVSSSIR-HLTTFVLTFAYVSTCLVKSQGVEGESKIAL 300
           D++  T+ LTR DI+ LR +V   S + +  L+TFV+T+AYV TC+VK +G +    + +
Sbjct: 248 DILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVCV 307

Query: 301 SFLVDWRSRVGTFGGVNYFGNGVSGYAVF-VKGREFLEE---NAVVMVAKKISEGITEI- 360
            F  D+RSR+       +FGN + G   F VK    LEE      +   + ++  +  + 
Sbjct: 308 GFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEGKGFITAVETLTGWVNGLC 367

Query: 361 TENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGWGSCKKVEMVSIN 420
            EN+  EK+++  FE     +++  P  + I V GS R G+Y  DFGWG   KVE+V+I+
Sbjct: 368 PENI--EKNMLLPFE----AFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTID 427

Query: 421 PSGSFSMADNKNGNGGVELGIALSENLMNVFSSLFFDGLK 450
              S S++++ +G+GGVE+G+ L ++ +  F SLF  GL+
Sbjct: 428 KDASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIGLE 449

BLAST of HG10000346 vs. TAIR 10
Match: AT5G39080.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-69
Identity = 174/466 (37.34%), Postives = 261/466 (56.01%), Query Frame = 0

Query: 1   MEVLEVCKIAPSPSPSTD-FSLPLTFFDLMFFLSPPTERILFYSFPQNEHLLLQFDSVL- 60
           + ++EV ++ PS S S +  SLPLT+FDL+++     ER++FY           FDSV+ 
Sbjct: 5   LNIIEVARVTPSNSDSAESLSLPLTYFDLIYYKLRAVERVIFYRITNVTRPF--FDSVIV 64

Query: 61  QNLKHSLSLTLSHFLPLAGNLVWPNHSPHPFILYKPGDSVSLTIAKTDADFNHLSSSHLK 120
            NLK SLS  LSH+LPLAG L+W      P I+Y   D VS ++A+T+ADF+ LS +   
Sbjct: 65  PNLKTSLSSCLSHYLPLAGKLIWEPLDHKPTIVYSQNDDVSFSVAETNADFSSLSGNEPF 124

Query: 121 KANQSHFLVPHLPISHTISPAMSLQITLFPNSGFSIGIITNHVIADGKTSTMFLKSWASI 180
            + + + LVP L  S   +  +S Q+TLFPN GF IG+  +H + DGKT+TMFLKSWA I
Sbjct: 125 PSTELYPLVPALQSSDDSASIVSFQVTLFPNQGFCIGVSAHHAVLDGKTTTMFLKSWAHI 184

Query: 181 SKTNNNHHLPP---PIFDRTAAINDPINGLQSLYNKY--FKTFI------SNPKSRLTSL 240
            K + +  LP    P +DRT  I  P +    + N++  F   +      +NPKS    L
Sbjct: 185 CK-HQDFSLPQDLIPTYDRT-VIKSPTDSENKVLNEWRSFTKILAGGKEPANPKS--LKL 244

Query: 241 TSKMIISDDVVSGTFELTRADI----EHLRRKVNEVSSSIR-----HLTTFVLTFAYVST 300
                I  DVV  T +LTR DI    E L+R+V+  SSS        L+TFV+ ++YV  
Sbjct: 245 NPSFEIGPDVVRYTLQLTREDIQTLRERLKREVSSSSSSTSSSKELRLSTFVIVYSYVLV 304

Query: 301 CLVKSQGVEGESKIALSFLVDWRSRVGTFGGVNYFGNGVSGYA-VFVKGREFLEENAVVM 360
           C+++++G E    +  +F VD RS +      NYFGN ++G + + +  + F+ E  ++ 
Sbjct: 305 CIIRARGGEPHRPVGYAFSVDCRSLMNP-PTPNYFGNCIAGCSRMMLTAKMFMGEEGLLA 364

Query: 361 VAKKISEGITEITENVENEKSLVEMFEENAMQWRKEMPIAKTIVVGGSPRFGVYEIDFGW 420
            A  +S+ I E  E+   +         + + +    P  + I+V GS RFGVYE+DFGW
Sbjct: 365 AATMVSDSIEEWDESFAWK-------IPDFVAYATLPPETQLILVSGSNRFGVYELDFGW 424

Query: 421 GSCKKVEMVSINPSGSFSMADNKNGNGGVELGIALSENLMNVFSSL 444
           G   KV +VSI+P    SMA++++ NG VE+G +L ++ M+    L
Sbjct: 425 GRPDKVMVVSISPGNGISMAESRDQNGSVEIGFSLKKHEMDTLIDL 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901948.11.4e-21385.08phenolic glucoside malonyltransferase 1-like [Benincasa hispida][more]
XP_022943466.19.1e-16066.38malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [... [more]
KAG7010988.12.7e-15965.58Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase, partial [Cucur... [more]
XP_023512233.15.9e-15965.58malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like [Cucurbita... [more]
XP_023522403.15.9e-15965.58malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like, partial [... [more]
Match NameE-valueIdentityDescription
Q940Z57.4e-7237.58Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1... [more]
Q9LJB41.8e-7037.83Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis ... [more]
Q9LRQ71.8e-7037.67BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2... [more]
Q9LRQ81.9e-6737.26Phenolic glucoside malonyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PMAT2... [more]
Q9FNP95.3e-6232.60Agmatine coumaroyltransferase OS=Arabidopsis thaliana OX=3702 GN=ACT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FRS64.4e-16066.38malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1J6U62.0e-15765.14malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1FXM33.3e-15564.92malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1FVL41.3e-14862.31malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase-like OS=Cucurbi... [more]
A0A6J1JFR01.7e-14361.87phenolic glucoside malonyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G39090.16.7e-7639.22HXXXD-type acyl-transferase family protein [more]
AT5G39050.15.3e-7337.58HXXXD-type acyl-transferase family protein [more]
AT3G29680.11.3e-7137.67HXXXD-type acyl-transferase family protein [more]
AT3G29590.11.3e-7137.83HXXXD-type acyl-transferase family protein [more]
AT5G39080.12.1e-6937.34HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 346..373
NoneNo IPR availablePANTHERPTHR31625FAMILY NOT NAMEDcoord: 1..449
NoneNo IPR availablePANTHERPTHR31625:SF50MALONYL-COA:ISOFLAVONE 7-O-GLUCOSIDE MALONYLTRANSFERASEcoord: 1..449
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 233..452
e-value: 1.8E-41
score: 143.7
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..220
e-value: 7.4E-61
score: 207.5
IPR003480TransferasePFAMPF02458Transferasecoord: 9..442
e-value: 2.7E-53
score: 181.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000346.1HG10000346.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups