HG10000335 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000335
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAminoacyl-tRNA hydrolase
LocationChr09: 3939902 .. 3943478 (-)
RNA-Seq ExpressionHG10000335
SyntenyHG10000335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTGGCATGGCTGAGTGCCATTGTAGTTGGGGCTGGCTGCCTTGTTTTGGGATACTATATTGGTTCGAAGTATCCTCCTCGCATCTTTGTCAAAGCCAAATTAGCCAAAAGCAATGAATCCAGCAAAAATGGGAAGAAGGATAAGCCTAAAGAGCCACTGGAGGTTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTCTGCCCCTGATTGTCTTTGTCTATCTCTGTTCCTCTCGTGATATCTATTAGAAAATCATTCTTGATTACTTTAAGATGTTCTCTCTCTCTCTCTCTCTCTCTAAAAAATGTAACAATACTTCACTGTGTCTCTTCAGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCAGCCCAATGCAGGTATTTTATTACCACCTTGTGGTTGCTCTTTCATTACTTGATATTTTAAATGCTAATTGCTAACAATGACAGGTTTTAAATTTTACTGCTGCTTGCATAGTGTGATTGCTGGAGTTGTTTTGTTTTGTTTTTGGTTTTTTAAATTAAAAACTAGTACTAAGAATTGGGCATATATAGTGTTTAGAAGTTTAGGTTAACAGCCCACTGCTCACCTGGTATATTAAAATCCTCACAATGACTATAATAATTTAATAAAAATTTATCAACAACTATAATAACATGAGACGTTTGGGATGAAAATAGGATTCAAGTCAAAGCTGTCAAAAAGCAGAATTTTGAAGATAATAGTGCTTCTATTCTTTTGAAATATTACACTGTCAAAATCAGGAAAATTATTGATGAATGGAATCTAAAAAACAACAGTAAATTTGCTAAGGGGAAGAATGCAGAAAATCCTCGTAAATATACTTTCAGAAAATCAGCAAACTTAGACTAAATCTCTATTGGAAATGAGACAGTGGAGGAAAAGTGACATAAACATGAAAAAGGTTGGGTATGATAAAGTGGGAAGTAAAGCAGGTTGGGTCAGAAATTGAGAAAGGTGAAGAGGGTGCATCATCAATACATCTTTCTTTCTTCATGGGATTGAGGCAAGGGGATTCAGTTACCCCTTTTCTTTACTATGAGGTTCAACCTAGGGAGGGGTGGAGTCTTGACAATATTATCCTATGAATACCAGCCATTTTTAAGATTAGTTTACTGTTTAAGGAGGATTCTCTCGTCCTTGATTATCTTTCCACAGATTTCGATGCGTGCCTACCCATTCAAAGGGATGGAGATTGTGTTTACTCAAAATAGTCCTATGTCATAAGGATTCAAGCTCGAGGGCAAAACGCCAAAGCCATTTCGCCAATAGAGCTTTGTTGCATAACCTTAAATTACCAATCTCAAGCACCTCTTCCCTCCCTCCCTCCCTTCCATTCACAAAATTCCCCACGACTTTTCAGCAAACTAGATGAGAACCATTCCCTTCTTCCACCCTTTCCCAAAGGAAGTCTCTCATGAGCTTCTCCTTGCTTTTACAAACCACAAAACAGGCGCTTGAAAAAGAGAGAGGTTAGATAGGAATTCCACTCAACACAAATCTGATCAAAGTAAGTCTACTAGCTTTAGAGAAAAAAGTTCTCTTCCACGAGGCTAACGTTTTCTGAATCTTCTCAATGAGACCATTCTAGAAAGAGATATCTCTTGGGTTACCTCCGAGAGGAAGACCCAGGTAGGAGGAAGGGAAAGAGCCAACCTCACAGCCCATCTAAGCTTCATCGAATCACAATTTAACCCCAAAATCTGACATTTACTCTGGTTAATTTTAAGCCTCGACATTGTCTCAAAGAACCTTAAAATGTGATTTAGAATAAGGAAGGACTCCTCTTTGCCAAAACTAAAGAACATTGTATGGTTAGCAAATTGCAGATGAGACAATGCAACCTCCTTAAAACCAACCTTAAAAGACTCAAGAATGCTTTCTTCCACTCCGCTAATAACATATGGCTCAGAACATCCACAACCCATATTACATTATTGCAATTATCAGCCAACTTTGTGGCCGATGTTTACCAGAAGAATTACCAATCCTACACAAACATTTTATGTTACTACTACTTGAGAGTCCAAGATTTCATCTTATTTACTACGAGTATGACTATATAAGTACCGACTTATTACTTGTTTAATAGTTAAATTATGAGAATCGAGACTAGTGATCTAATTTTCACAAATCTTTGCATTTTAAACTTTTCAGTTAATGTTGTAAATTGGATTTGGAATCATTTTTAATCTTACTTGCTTTTATTAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCCTTGAATAGGTATGTATTTACAGAATAGTCCACCCTATATTAATATGTGTTGCATACTTTGTTCTTTGTACATTTATTTGATTTTATTTACATGTAGATGGGAGATGTGTGCGCAACCCAAGGTGGTTCTGAAGATAGAAAGTGAGGAAGACATGCTAGTTTTGCAAGTAACAATTCCACTATGATTCGGGCTGGTTTAAGCAATTGATTATTTATATTAGGAGGTGTAAGCAATTGATTCGGGCTGGTTTCTTGCTGAAACCAATTTTTTTCCAAACTGACCAACTCACTATTTTTTTTTATTTGACTGGATTAATCGGCTATTGTACTTCAAAAGAGCCAAACTATTAATTAAGACGTATGTTCCTTGCTTGCACCCTGCCAACCCCCTTCATATTTATTAAGACATTTATCCTTGTACCACCTTACCTATAGTCGGCTTCTTTTTCGCTCCATTAGATCTATTTTTCTTACCAACTCTAGCATAGTGCCATGAGCATAATATTATACAATTCGTTCTAGTGTTTATTTCTGGATAGCCTTCTTTATAATCTTTCCTTTTCTTCCCAGTTAATTTTTCATCTATCTGAGACAATTCAATGTTGCTTGCTCTTTTCTATATCTCATCTATTCTTTGAGGCCTTTGAGTTAGTTATTTCATTTTTCAAATATTTATATTATCGTACATCAGGAGAGGGCAAAATCATTGAAGCTACCAACACATATTACCATTGATGCCGGAAGAACTCAGATTGCTCCAAGTAAGTTATATGAAATTCACTCAAGTAGATCGATTTTTTCCACAGCAAATTTATCAAGTTCACCAATTTTTCACCTTTTTGTTCTTTCAGATTCGAGAACGGTGATGGCAATTCTTGGTAAGTAGATTTCTTCCATGCAACGTTTATAAATCAAAGAGTCTTTGTTTCTGCTGTATTCAATTTGAGCATTCCTTTTTTAGCAAAATTCCTAAGATAGGAAGAATGCTCCCTCAAACTTATTTTGAAATAAAACGTTCACTACTTTAAGACCTTGCCATTGGTTTCTATCTATATTCTACCAACTCAGCAATCACTCTTTTGTTTGACTCCTATTTTTGGTTAGCTTAACTGGTGGATCTACTTAACAATTTCATTTTGGTGCTAACTAAAATTAATATATACTAATTTGGTTCCATTGAATATGATAGCATACACTTCTTACTTCCAAATTGCAGGCCCTGTTGAAGTTGTTGATGATGTAACTGGTGGGTTGAAACTTTTGTAG

mRNA sequence

ATGGATCTGGCATGGCTGAGTGCCATTGTAGTTGGGGCTGGCTGCCTTGTTTTGGGATACTATATTGGTTCGAAGTATCCTCCTCGCATCTTTGTCAAAGCCAAATTAGCCAAAAGCAATGAATCCAGCAAAAATGGGAAGAAGGATAAGCCTAAAGAGCCACTGGAGGTTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCAGCCCAATGCAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCCTTGAATAGATGGGAGATGTGTGCGCAACCCAAGGTGGTTCTGAAGATAGAAAGTGAGGAAGACATGCTAGTTTTGCAAGAGAGGGCAAAATCATTGAAGCTACCAACACATATTACCATTGATGCCGGAAGAACTCAGATTGCTCCAAATTCGAGAACGGTGATGGCAATTCTTGGCCCTGTTGAAGTTGTTGATGATGTAACTGGTGGGTTGAAACTTTTGTAG

Coding sequence (CDS)

ATGGATCTGGCATGGCTGAGTGCCATTGTAGTTGGGGCTGGCTGCCTTGTTTTGGGATACTATATTGGTTCGAAGTATCCTCCTCGCATCTTTGTCAAAGCCAAATTAGCCAAAAGCAATGAATCCAGCAAAAATGGGAAGAAGGATAAGCCTAAAGAGCCACTGGAGGTTGAAAGTTTGGCTGACATTCTCGATGACTTCAAGATGGTTTTAGTTGTGAGAAATGATTTGAAAATGGGAAAAGGAAAAATTGCAGCCCAATGCAGTCATGCAACTTTGGGTCTCTATAAGAAGCTCCATTATCGTGCACCAAAAGCCTTGAATAGATGGGAGATGTGTGCGCAACCCAAGGTGGTTCTGAAGATAGAAAGTGAGGAAGACATGCTAGTTTTGCAAGAGAGGGCAAAATCATTGAAGCTACCAACACATATTACCATTGATGCCGGAAGAACTCAGATTGCTCCAAATTCGAGAACGGTGATGGCAATTCTTGGCCCTGTTGAAGTTGTTGATGATGTAACTGGTGGGTTGAAACTTTTGTAG

Protein sequence

MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Homology
BLAST of HG10000335 vs. NCBI nr
Match: XP_038901927.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 342.8 bits (878), Expect = 1.8e-90
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNG-KKDKPKEPLEVES 60
           MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFV+AKLAKSNES +NG KKDKPKEPLEVE+
Sbjct: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVRAKLAKSNESGRNGKKKDKPKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

BLAST of HG10000335 vs. NCBI nr
Match: XP_004148093.1 (peptidyl-tRNA hydrolase 2, mitochondrial [Cucumis sativus] >XP_008459200.1 PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial-like [Cucumis melo] >KAA0042983.1 peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa] >KGN63396.1 hypothetical protein Csa_022142 [Cucumis sativus])

HSP 1 Score: 342.0 bits (876), Expect = 3.0e-90
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNG-KKDKPKEPLEVES 60
           MDLAWLSAIVVGAGCLVLGYYIGSKYPPR+FVKAKLAKSNESS+NG KKDK KEPLEVE+
Sbjct: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRVFVKAKLAKSNESSRNGKKKDKTKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

BLAST of HG10000335 vs. NCBI nr
Match: XP_022148652.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Momordica charantia])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 172/180 (95.56%), Postives = 177/180 (98.33%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSAIVVGAGCLVLGYYIGSKYPPRIF KAKLAKSNESS++GKKDKPKE LE+ESL
Sbjct: 1   MDLTWLSAIVVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMA+LGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

BLAST of HG10000335 vs. NCBI nr
Match: KAG7035146.1 (Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 3.4e-89
Identity = 172/180 (95.56%), Postives = 177/180 (98.33%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSAIVVGAGCLVLGY IGSKYPPRIF+KAKLAKSNESS++GKK KPKEPLEVE+L
Sbjct: 1   MDLTWLSAIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRSGKKGKPKEPLEVENL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADIL+DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILEDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

BLAST of HG10000335 vs. NCBI nr
Match: XP_022947215.1 (peptidyl-tRNA hydrolase 2, mitochondrial-like [Cucurbita moschata] >XP_022947216.1 peptidyl-tRNA hydrolase 2, mitochondrial-like [Cucurbita moschata] >XP_022947217.1 peptidyl-tRNA hydrolase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 338.2 bits (866), Expect = 4.4e-89
Identity = 172/180 (95.56%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSAIVVGAGCLVLGY IGSKYPPRIF+KAKLAKSNESS+ GKK KPKEPLEVE+L
Sbjct: 1   MDLTWLSAIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRTGKKGKPKEPLEVENL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADIL+DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILEDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

BLAST of HG10000335 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.8e-30
Identity = 73/137 (53.28%), Postives = 91/137 (66.42%), Query Frame = 0

Query: 47  KKDKPKEPLEVESLADILDD---FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRA 106
           K    K   + ES A IL D   +KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R 
Sbjct: 42  KSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRN 101

Query: 107 PKALNRWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI 166
           P+ L +WE C QPKVV+K   EE ++ L   AK L L   +  DAGRTQIAP S+TV+ I
Sbjct: 102 PEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGI 161

Query: 167 -LGPVEVVDDVTGGLKL 180
             GP +++D VTG LKL
Sbjct: 162 GPGPADLIDKVTGHLKL 178

BLAST of HG10000335 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-29
Identity = 70/128 (54.69%), Postives = 88/128 (68.75%), Query Frame = 0

Query: 56  EVESLADIL---DDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEM 115
           + E+ A IL    ++KM+LVVRNDLKMGKGK+AAQCSHA +  YK++  R P+ L  WE 
Sbjct: 51  DTETEASILGESGEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEY 110

Query: 116 CAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI-LGPVEVVD 175
           C QPKVV+K   EE ++ L   AK L L   +  DAGRTQIAP SRTV+ I  GP +++D
Sbjct: 111 CGQPKVVVKAPDEETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADLID 170

Query: 176 DVTGGLKL 180
            VTG LKL
Sbjct: 171 KVTGHLKL 178

BLAST of HG10000335 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-29
Identity = 65/115 (56.52%), Postives = 83/115 (72.17%), Query Frame = 0

Query: 66  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESE 125
           ++KM+LVVR DLKMGKGK+AAQCSHA +  YK+   R+P+ L  WE C QPKVV+K   E
Sbjct: 66  EYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDE 125

Query: 126 EDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI-LGPVEVVDDVTGGLKL 180
           + ++ L   AK+L L   +  DAGRTQI P SRTV+ I  GPVE++D+VTG LKL
Sbjct: 126 DTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKL 180

BLAST of HG10000335 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 69/180 (38.33%), Postives = 106/180 (58.89%), Query Frame = 0

Query: 10  VVGAGCLVLGYYIGSKYPPR-------IFVKAKLAKSNESSKNGKKDKPKEPLEVESLAD 69
           ++    ++L +++G +Y  +       +   A    S ESS +   +     +  +    
Sbjct: 11  IINGLAVMLSFFVGYRYALKRGDAKDSVTEGAATPFSQESSVSSGSE---ASVSDKGYGG 70

Query: 70  ILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKI 129
           + D+FKMVLVVRNDLKMGKGKIAAQC H  +G Y++   R P+ L  WE C   K+ +++
Sbjct: 71  LNDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRV 130

Query: 130 ESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEV--VDDVTGGLKLL 181
           ESE +++ +++ A+  +L T +  DAGRTQI  NS+TV+A+ GP     +D VTG LKLL
Sbjct: 131 ESEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAV-GPAAAADIDRVTGHLKLL 186

BLAST of HG10000335 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-23
Identity = 77/193 (39.90%), Postives = 105/193 (54.40%), Query Frame = 0

Query: 7   SAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKP---KEPLEVESLADI 66
           +A++VGA   V+G+ IG KY      +   +K+   + N +K+ P       + ES  ++
Sbjct: 15  AAVLVGA---VVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESETEL 74

Query: 67  LD----------------DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKAL 126
           LD                  KMVLVVR DL M KGKIAAQC+HA L  YK      P  +
Sbjct: 75  LDMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLV 134

Query: 127 NRWEMCAQPKVVLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI-LGP 180
             WE   Q K+ L+ ++EE + +LQ +A SL L   +  DAGRTQIA  S TV+ I  GP
Sbjct: 135 RIWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGPGP 194

BLAST of HG10000335 vs. ExPASy TrEMBL
Match: A0A5A7TI30 (Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00570 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNG-KKDKPKEPLEVES 60
           MDLAWLSAIVVGAGCLVLGYYIGSKYPPR+FVKAKLAKSNESS+NG KKDK KEPLEVE+
Sbjct: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRVFVKAKLAKSNESSRNGKKKDKTKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

BLAST of HG10000335 vs. ExPASy TrEMBL
Match: A0A0A0LTR6 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G437060 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNG-KKDKPKEPLEVES 60
           MDLAWLSAIVVGAGCLVLGYYIGSKYPPR+FVKAKLAKSNESS+NG KKDK KEPLEVE+
Sbjct: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRVFVKAKLAKSNESSRNGKKKDKTKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

BLAST of HG10000335 vs. ExPASy TrEMBL
Match: A0A1S3C945 (Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103498393 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.5e-90
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNG-KKDKPKEPLEVES 60
           MDLAWLSAIVVGAGCLVLGYYIGSKYPPR+FVKAKLAKSNESS+NG KKDK KEPLEVE+
Sbjct: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRVFVKAKLAKSNESSRNGKKKDKTKEPLEVEN 60

Query: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120
           LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV
Sbjct: 61  LADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVV 120

Query: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180
           LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL
Sbjct: 121 LKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKL 180

BLAST of HG10000335 vs. ExPASy TrEMBL
Match: A0A6J1D5N0 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.6e-90
Identity = 172/180 (95.56%), Postives = 177/180 (98.33%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSAIVVGAGCLVLGYYIGSKYPPRIF KAKLAKSNESS++GKKDKPKE LE+ESL
Sbjct: 1   MDLTWLSAIVVGAGCLVLGYYIGSKYPPRIFFKAKLAKSNESSRSGKKDKPKEALEIESL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLH+RAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHHRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMA+LGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAVLGPVEVVDDVTGGLKLL 180

BLAST of HG10000335 vs. ExPASy TrEMBL
Match: A0A6J1G686 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.1e-89
Identity = 172/180 (95.56%), Postives = 176/180 (97.78%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSAIVVGAGCLVLGY IGSKYPPRIF+KAKLAKSNESS+ GKK KPKEPLEVE+L
Sbjct: 1   MDLTWLSAIVVGAGCLVLGYCIGSKYPPRIFIKAKLAKSNESSRTGKKGKPKEPLEVENL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADIL+DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL
Sbjct: 61  ADILEDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL
Sbjct: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180

BLAST of HG10000335 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 248.1 bits (632), Expect = 5.6e-66
Identity = 134/182 (73.63%), Postives = 150/182 (82.42%), Query Frame = 0

Query: 1   MDLAW-LSAIVVGAGCLVLGYYIGS-KYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVE 60
           MDL W LS ++VGA    LGYYI + +   RIF+ +K    +  S   KK K KEPLE+E
Sbjct: 1   MDLVWLLSVLLVGA---ALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIE 60

Query: 61  SLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKV 120
            LAD   +FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE CAQPKV
Sbjct: 61  KLADFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKV 120

Query: 121 VLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLK 180
           V+KIESEE+MLVLQERAK+LKLPTHITIDAG+TQIAPNSRTVMAILGPV+ VD+VTGGLK
Sbjct: 121 VVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLK 179

BLAST of HG10000335 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 248.1 bits (632), Expect = 5.6e-66
Identity = 134/182 (73.63%), Postives = 150/182 (82.42%), Query Frame = 0

Query: 1   MDLAW-LSAIVVGAGCLVLGYYIGS-KYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVE 60
           MDL W LS ++VGA    LGYYI + +   RIF+ +K    +  S   KK K KEPLE+E
Sbjct: 1   MDLVWLLSVLLVGA---ALGYYISTLRQTRRIFLSSKSVAIDAGSSGNKKTKSKEPLEIE 60

Query: 61  SLADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKV 120
            LAD   +FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKALNRWE CAQPKV
Sbjct: 61  KLADFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLLQRAPKALNRWEYCAQPKV 120

Query: 121 VLKIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLK 180
           V+KIESEE+MLVLQERAK+LKLPTHITIDAG+TQIAPNSRTVMAILGPV+ VD+VTGGLK
Sbjct: 121 VVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSRTVMAILGPVDNVDEVTGGLK 179

BLAST of HG10000335 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 242.7 bits (618), Expect = 2.3e-64
Identity = 132/180 (73.33%), Postives = 144/180 (80.00%), Query Frame = 0

Query: 1   MDLAWLSAIVVGAGCLVLGYYIGSKYPPRIFVKAKLAKSNESSKNGKKDKPKEPLEVESL 60
           MDL WLSA++VGA    LGY IG++    +             K+ K    K PLE+E L
Sbjct: 1   MDLTWLSALIVGA---ALGYCIGTRRSKPVVAAV--------DKDIKTQSSKSPLEIEKL 60

Query: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVL 120
           ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKL  RAPKAL+ WE CAQPKVV+
Sbjct: 61  ADILDDFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVV 120

Query: 121 KIESEEDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDDVTGGLKLL 180
           KIE E++ML LQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVD+VTGGLKLL
Sbjct: 121 KIEDEDEMLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGPVEVVDEVTGGLKLL 169

BLAST of HG10000335 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 111.3 bits (277), Expect = 8.1e-25
Identity = 58/116 (50.00%), Postives = 80/116 (68.97%), Query Frame = 0

Query: 66  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESE 125
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE   QPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 126 EDMLVLQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI-LGPVEVVDDVTGGLKLL 181
           ++M  + E A+S+ LPT +  DAGRT++A  SRTV+A+  GP E+VD +TG L LL
Sbjct: 138 QEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPKELVDSITGRLALL 193

BLAST of HG10000335 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.0e-11
Identity = 31/63 (49.21%), Postives = 43/63 (68.25%), Query Frame = 0

Query: 66  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLHYRAPKALNRWEMCAQPKVVLKIESE 125
           + KMVLVVR DLKM  GKIA+QC+HA  G+Y +L       L RWE   QPK+V+  +++
Sbjct: 78  ELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLRRWEENGQPKIVVTCKNQ 137

Query: 126 EDM 129
           ++M
Sbjct: 138 QEM 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901927.11.8e-9097.24peptidyl-tRNA hydrolase 2, mitochondrial-like [Benincasa hispida][more]
XP_004148093.13.0e-9097.24peptidyl-tRNA hydrolase 2, mitochondrial [Cucumis sativus] >XP_008459200.1 PREDI... [more]
XP_022148652.11.2e-8995.56peptidyl-tRNA hydrolase 2, mitochondrial-like isoform X1 [Momordica charantia][more]
KAG7035146.13.4e-8995.56Peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022947215.14.4e-8995.56peptidyl-tRNA hydrolase 2, mitochondrial-like [Cucurbita moschata] >XP_022947216... [more]
Match NameE-valueIdentityDescription
Q9Y3E52.8e-3053.28Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S... [more]
Q3ZBL51.4e-2954.69Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=... [more]
Q8R2Y81.8e-2956.52Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ... [more]
O970672.1e-2538.33Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ... [more]
O138301.5e-2339.90Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Match NameE-valueIdentityDescription
A0A5A7TI301.5e-9097.24Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A0A0LTR61.5e-9097.24Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G437060 PE=4 SV=1[more]
A0A1S3C9451.5e-9097.24Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103498393 PE=4 SV=1[more]
A0A6J1D5N05.6e-9095.56Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111017259 PE=4 SV=... [more]
A0A6J1G6862.1e-8995.56Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451148 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G03010.15.6e-6673.63Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT3G03010.25.6e-6673.63Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT5G16870.12.3e-6473.33Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT4G32900.28.1e-2550.00Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT4G32900.13.0e-1149.21Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002833Peptidyl-tRNA hydrolase, PTH2TIGRFAMTIGR00283TIGR00283coord: 68..180
e-value: 1.6E-33
score: 113.4
IPR002833Peptidyl-tRNA hydrolase, PTH2PFAMPF01981PTH2coord: 68..180
e-value: 2.8E-41
score: 140.5
IPR002833Peptidyl-tRNA hydrolase, PTH2PANTHERPTHR12649PEPTIDYL-TRNA HYDROLASE 2coord: 3..180
IPR002833Peptidyl-tRNA hydrolase, PTH2CDDcd02430PTH2coord: 67..180
e-value: 1.97648E-62
score: 186.575
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 65..180
e-value: 2.1E-44
score: 152.3
IPR023476Peptidyl-tRNA hydrolase II domain superfamilySUPERFAMILY102462Peptidyl-tRNA hydrolase IIcoord: 65..180
NoneNo IPR availablePANTHERPTHR12649:SF19OSJNBA0060D06.11 PROTEINcoord: 3..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000335.1HG10000335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity