HG10000313 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000313 vs. NCBI nr
Match: XP_038901378.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 780/827 (94.32%), Postives = 795/827 (96.13%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPIS SE P PLLTRNTNA AQ RIGP+SRFSPRSSLIRLNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEASD        H HGHHH HHGCQHH  GD DGVELTGAQKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEASD--------HRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+SHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+SFEYFP
Sbjct: 481 IGGDKSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVR STYGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRASTYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPRP VVDAIAEL+D+GKLRVMMLTGDHDSSAWKVANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA RASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSIS VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNS+RALNQPSWSWKQDFQNL+ + RSKLN  PETSSGTI+TATS
Sbjct: 781 VCLNSVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTATS 819

BLAST of HG10000313 vs. NCBI nr
Match: XP_008462778.1 (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis melo])

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 778/827 (94.07%), Postives = 794/827 (96.01%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ SE P PLLT NTNA +Q RIGPVSRFSP SSLI LNRFPIC+KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEASD    H  GHHHGHHH HHGCQHH SGD D VELTG QKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEASD----HRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFP
Sbjct: 481 IGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IKDAVR STYGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNS+RALN PSWSWKQDFQNLI +ARSKLNTTPETSSGTI+TATS
Sbjct: 781 VCLNSVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of HG10000313 vs. NCBI nr
Match: XP_004151907.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis sativus] >KAE8652344.1 hypothetical protein Csa_021970 [Cucumis sativus])

HSP 1 Score: 1500.3 bits (3883), Expect = 0.0e+00
Identity = 775/827 (93.71%), Postives = 793/827 (95.89%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ SE P PLLT NTNA +Q R+GPVSRFSP SSLI LNRFP  +KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEAS     H HGHHHGHHH HHGCQHH SGD DGVELTGAQKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEAS----GHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEV+PLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFP
Sbjct: 481 IGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR IKDAVR STYGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNS+RALN PSWSWKQD QNLI DARSKLNTTPE SSGTI+TATS
Sbjct: 781 VCLNSVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 823

BLAST of HG10000313 vs. NCBI nr
Match: XP_038901377.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1497.6 bits (3876), Expect = 0.0e+00
Identity = 780/841 (92.75%), Postives = 795/841 (94.53%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPIS SE P PLLTRNTNA AQ RIGP+SRFSPRSSLIRLNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEASD        H HGHHH HHGCQHH  GD DGVELTGAQKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEASD--------HRHGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+SHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+SFEYFP
Sbjct: 481 IGGDKSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFP 540

Query: 593 GRGLIATLHGIK--------------SGIGGKLRKASLGSVDFITSFCKSENESRKIKDA 652
           GRGLIATLHGIK              SGIGGKLRKASLGSVDFITSFCKSENESRKIKDA
Sbjct: 541 GRGLIATLHGIKVEGEEYLHSISILQSGIGGKLRKASLGSVDFITSFCKSENESRKIKDA 600

Query: 653 VRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSA 712
           VR STYGSEFVHAALSVDQKVTLIHLEDQPRP VVDAIAEL+D+GKLRVMMLTGDHDSSA
Sbjct: 601 VRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSA 660

Query: 713 WKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAH 772
           WKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA 
Sbjct: 661 WKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLAR 720

Query: 773 RASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLP 832
           RASATATAVADVLLLQDSIS VPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLP
Sbjct: 721 RASATATAVADVLLLQDSISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLP 780

Query: 833 LWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTAT 880
           LWLTVLLHEGGTLLVCLNS+RALNQPSWSWKQDFQNL+ + RSKLN  PETSSGTI+TAT
Sbjct: 781 LWLTVLLHEGGTLLVCLNSVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTAT 833

BLAST of HG10000313 vs. NCBI nr
Match: XP_023533453.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 742/827 (89.72%), Postives = 770/827 (93.11%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ S+P   LLTR TNA A+ RIGPVS FS RSS+IRL R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
             FRCAA+ASD RH H       HHH HH CQHH  GDGDGVELTGAQKAFVRFA AIRW
Sbjct: 61  --FRCAADASDHRHGH-------HHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAKAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLHLCCGSAALF+TAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATIT
Sbjct: 241 SHPDFALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFP
Sbjct: 481 IGGDNSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPR  V+DAIAEL+D+  LRVMMLTGDHDSSAWK+ANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRAGVLDAIAELQDQANLRVMMLTGDHDSSAWKIANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNSIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 VCLNSIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 805

BLAST of HG10000313 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 574/802 (71.57%), Postives = 653/802 (81.42%), Query Frame = 0

Query: 81  RIGPVSRFS--PRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHHHG-----HH 140
           R+  V+ FS  P  +L+R     I       PR    R      D  H HHH      H+
Sbjct: 25  RLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRA---VEDHHHDHHHDDEQDHHN 84

Query: 141 HGHHHGHHGCQHHSSGDGDGVEL---TGAQKAFVRFADAIRWTDLANYLREHLHLCCGSA 200
           H HHH  HGC          VEL   +  QK    FA AI W  LANYLREHLHLCC +A
Sbjct: 85  HHHHHHQHGC--------CSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAA 144

Query: 201 ALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAA 260
           A+F+ AA CPYL P+P +K LQN F+ V FPLVGVSASLDAL D++GGKVNIHVLMALAA
Sbjct: 145 AMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAA 204

Query: 261 FASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPD 320
           FAS+FMGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+
Sbjct: 205 FASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPN 264

Query: 321 TSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGER 380
            SDL ++ VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPL+ K G+R
Sbjct: 265 ISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDR 324

Query: 381 VPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVL 440
           VPGGARNLDGR+IVKATKAW +STL++IVQLTEEA  NKPKLQRWL+EFGENYSKVVVVL
Sbjct: 325 VPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVL 384

Query: 441 SVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGI 500
           S+A+A +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGI
Sbjct: 385 SLAIAFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGI 444

Query: 501 LLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEK 560
           LLKG  VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEK
Sbjct: 445 LLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEK 504

Query: 561 EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKS-GIGG 620
           EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSI V+SFEYFPGRGL AT++G+K+     
Sbjct: 505 EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEES 564

Query: 621 KLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPR 680
           +LRKASLGS++FITS  KSE+ES++IKDAV  S+YG +FVHAALSVDQKVTLIHLEDQPR
Sbjct: 565 RLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPR 624

Query: 681 PEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISRE 740
           P V   IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE
Sbjct: 625 PGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIARE 684

Query: 741 MGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQ 800
            GGGLIMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQ
Sbjct: 685 AGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQ 744

Query: 801 TTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWK 860
           TTSLVKQNV LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWK
Sbjct: 745 TTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWK 804

Query: 861 QDFQNLICDARSKLNTTPETSS 872
           QD  +LI   RS+  T+  ++S
Sbjct: 805 QDIVHLINKLRSQEPTSSSSNS 814

BLAST of HG10000313 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 3.4e-73
Identity = 198/682 (29.03%), Postives = 348/682 (51.03%), Query Frame = 0

Query: 166 FADAIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGV 225
           F D   +TD  +++R++  L    + LF+       ++          +FI   F + G 
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFIAVGYASQIMNGEDFYLTNALFIFAIF-IGGY 131

Query: 226 SASLDALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMI 285
           S   +   +L   +  +  LM +A   + F+G   EG +++++F ++   E Y   ++  
Sbjct: 132 SLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKARQ 191

Query: 286 DVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVY 345
            ++ L +  P  ALV     D          R V V D+++G  +++  G+ + +D  V 
Sbjct: 192 SIRSLMDIAPKEALVRRSGTD----------RMVHVDDIQIGDIMIIKPGQKIAMDGHVV 251

Query: 346 QGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEA 405
           +G + +    +TGE  P++  + + V  G  N +G + V  TK  E++T+S+I+ L EEA
Sbjct: 252 KGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEEA 311

Query: 406 QLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVA 465
           Q  +   Q +++ F + Y+  ++V++  +A + PLLF         +   VY+ L ++V 
Sbjct: 312 QGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLF------GGNWETWVYQGLSVLVV 371

Query: 466 ASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTG-GLVFK 525
             PCAL  + P+A   AI + A+ G+L+KGG  L+ +     +AFDKTGTLT G  +V  
Sbjct: 372 GCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGTLTKGVPVVTD 431

Query: 526 AIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK--DLP 585
            IE      I  ++++               + AA+E+ + HP+  A++ +   +  DL 
Sbjct: 432 YIELTEATNIQHNKNYI--------------IMAALEQLSQHPLASAIIKYGETREMDLT 491

Query: 586 SISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR-KIKDAVR 645
           SI+V+ F    G+G+  T+ G    +G  +    L +  F  S  +  ++ + K K A+ 
Sbjct: 492 SINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLASQFTDSIHRQVSDLQLKGKTAML 551

Query: 646 TSTYGSEFVHAALSVDQK-VTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAW 705
             T            +QK ++++ + D+ R      I  L + G  + +MLTGD+ ++A 
Sbjct: 552 FGT------------NQKLISIVAVADEVRSSSQHVIKRLHELGIEKTIMLTGDNQATAQ 611

Query: 706 KVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHR 765
            +   VG++E+   L P+DKL ++K++     G + MVG+GINDAPALAAATVGI +   
Sbjct: 612 AIGQQVGVSEIEGELMPQDKLDYIKQLKINF-GKVAMVGDGINDAPALAAATVGIAMGGA 671

Query: 766 ASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPL 825
            + TA   ADV L+ D +  +PF +  SR+T  ++KQN+T +L   L+A L  + G+L L
Sbjct: 672 GTDTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIALLLVIPGWLTL 707

Query: 826 WLTVLLHEGGTLLVCLNSIRAL 842
           W+ ++   G TLLV LN +R +
Sbjct: 732 WIAIMADMGATLLVTLNGLRLM 707

BLAST of HG10000313 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.5e-73
Identity = 184/632 (29.11%), Postives = 328/632 (51.90%), Query Frame = 0

Query: 214 VFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 273
           +   +A  + G +   +   +L      +  LM +A   +  +G   EG +++++F  + 
Sbjct: 117 ILYVIAIVVGGFNLFKEGFANLIKLDFTMESLMTIAIIGASIIGEWAEGSIVVILFAFSE 176

Query: 274 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 333
           + E Y   ++   ++ L +  P  AL+            D   + + V D+++G  +++ 
Sbjct: 177 VLERYSMDKARQSIRSLMDIAPKEALI----------RRDDVEQMIAVSDIQIGDIMIIK 236

Query: 334 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 393
            G+ + +D  V +G + I    +TGE  P++ KV + V  G  N +G + VK TK  E++
Sbjct: 237 PGQKIAMDGVVIKGYSAINQSAITGESIPVEKKVDDEVFAGTLNEEGLLEVKVTKHVEDT 296

Query: 394 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 453
           T+S+I+ L EEAQ  +   Q ++++F + Y+  ++++++ V ++ PL F   +       
Sbjct: 297 TISKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMLIALLVVVVPPLFFGGDWDTW---- 356

Query: 454 GSVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 513
             VY+ L L+V   PC+L  + P++   AI + A+ G+L+KGG  L+ +     +AFDKT
Sbjct: 357 --VYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLQAIAFDKT 416

Query: 514 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 573
           GTLT G  V     P   H                 E+ +L++  A+E  + HP+  A++
Sbjct: 417 GTLTKGKPVVTDFIPYSEH---------------MDEQNSLSIITALETMSQHPLASAII 476

Query: 574 DHSV--GKDLPSISVDSFEYFPGRGLIATLHGIKSGIG-GKLRKASLGSVDFITSFCKSE 633
             ++    D  SI +D+F    G+G+   ++GI   IG  KL ++SL     I+   +S 
Sbjct: 477 SKAMIDNVDYKSIEIDNFSSITGKGVKGEVNGITYYIGSSKLFESSLEKSQSISQTYQSL 536

Query: 634 NESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMM 693
            +  K         +G+E           + +I + D+ R    + IA+L   G    +M
Sbjct: 537 QKQGK-----TAMLFGTE--------SNILAIIAVADEVRESSKEVIAQLHKLGIAHTIM 596

Query: 694 LTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAA 753
           LTGD++ +A  +   +G++++   L PEDKL ++KE+ ++  G + M+G+G+NDAPALAA
Sbjct: 597 LTGDNNDTAQFIGKEIGVSDIKAELMPEDKLTYIKEL-KQTYGKVAMIGDGVNDAPALAA 656

Query: 754 ATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLAS 813
           +TVGI +    + TA   ADV L+ D +  +PF +  SR+T  ++KQN+T +L   LLA 
Sbjct: 657 STVGIAMGGAGTDTALETADVALMGDDLKKLPFIVNLSRKTLKIIKQNITFSLGIKLLAL 703

Query: 814 LPSVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 842
           L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 717 LLVLPGWLTLWIAIVADMGATLLVTLNGLRLM 703

BLAST of HG10000313 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.5e-71
Identity = 177/605 (29.26%), Postives = 318/605 (52.56%), Query Frame = 0

Query: 241 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 300
           ++  LM +A   +  +G   E  +++V+F ++   E +   ++   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 301 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 360
           +    + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 361 KPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFG 420
            P+   V + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++++F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 421 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 480
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 399

Query: 481 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESH 540
            AI + A+KG+L+KGG  L+ + +   +AFDKTGTLT      K +  +   K+  D+  
Sbjct: 400 SAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQVE 459

Query: 541 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDL--PSISVDSFEYFPGRGLI 600
                    EKE  ++  A+E  + HP+  A++  +   ++    + V+ F    GRG+ 
Sbjct: 460 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQ 519

Query: 601 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQ 660
             + G    IG       L   DF   F   EN+ + +++  +T+          +  DQ
Sbjct: 520 GNIDGTTYYIGSPRLFKELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIGTDQ 579

Query: 661 KVT-LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 720
            +  +I + D+ R    + I +L   G  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 580 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMP 639

Query: 721 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 780
           +DKL ++K++  E  G + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 640 QDKLDYIKKMKAE-HGNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 699

Query: 781 ISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 840
           +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 700 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 721

Query: 841 SIRAL 842
           S+R +
Sbjct: 760 SLRLM 721

BLAST of HG10000313 vs. ExPASy Swiss-Prot
Match: P20021 (Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.6e-70
Identity = 179/606 (29.54%), Postives = 318/606 (52.48%), Query Frame = 0

Query: 241 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 300
           ++  LM +A   +  +G   E  +++++F ++   E +   +S   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 301 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 360
                + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 361 KPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFG 420
            P+   V + V  G  N +G I VK TK  E++T+++I+ L EEAQ  +   Q ++++F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 421 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 480
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 400

Query: 481 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESH 540
            AI + A+KG+L+KGG  L+ + +  TVAFDKTGTLT      K +  +   ++  D+  
Sbjct: 401 SAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLT------KGVPVVTDFEVLNDQVE 460

Query: 541 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLP--SISVDSFEYFPGRGLI 600
                    EKE  ++  A+E  + HP+  A++  +   ++P  ++ V+ F    GRG+ 
Sbjct: 461 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIK 520

Query: 601 ATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQ 660
             ++G    IG       L   DF   F   EN  + +++  +T         A +   +
Sbjct: 521 GIVNGTTYYIGSPKLFKELNVSDFSLGF---ENNVKILQNQGKT---------AMIIGTE 580

Query: 661 KVTL--IHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLK 720
           K  L  I + D+ R    + I +L   G  + +MLTGD+  +A  +   VG++++   L 
Sbjct: 581 KTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTANAIGTHVGVSDIQSELM 640

Query: 721 PEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQD 780
           P+DKL ++K++  E    + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D
Sbjct: 641 PQDKLDYIKKMQSEY-DNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGD 700

Query: 781 SISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCL 840
            +S +PF +  SR+T +++K N+T A+   ++A L  + G+L LW+ +L   G T+LV L
Sbjct: 701 DLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVAL 722

Query: 841 NSIRAL 842
           NS+R +
Sbjct: 761 NSLRLM 722

BLAST of HG10000313 vs. ExPASy TrEMBL
Match: A0A1S3CI84 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501066 PE=3 SV=1)

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 778/827 (94.07%), Postives = 794/827 (96.01%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ SE P PLLT NTNA +Q RIGPVSRFSP SSLI LNRFPIC+KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEASD    H  GHHHGHHH HHGCQHH SGD D VELTG QKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEASD----HRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFP
Sbjct: 481 IGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IKDAVR STYGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNS+RALN PSWSWKQDFQNLI +ARSKLNTTPETSSGTI+TATS
Sbjct: 781 VCLNSVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of HG10000313 vs. ExPASy TrEMBL
Match: A0A0A0LN89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433360 PE=3 SV=1)

HSP 1 Score: 1480.3 bits (3831), Expect = 0.0e+00
Identity = 769/827 (92.99%), Postives = 787/827 (95.16%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ SE P PLLT NTNA +Q R+GPVSRFSP SSLI LNRFP  +KKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
            HFRCAAEAS     H HGHHHGHHH HHGCQHH SGD DGVELTGAQKAFVRFA+AIRW
Sbjct: 61  GHFRCAAEAS----GHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATIT
Sbjct: 241 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGEV+PLQIKVGERVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+S FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISV+S EYFP
Sbjct: 481 IGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESR IKDAVR STYGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPRP V+DAIAEL+  GKLRVMMLTGDHDSSAWKVANAVGINEVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLG     L VLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNS+RALN PSWSWKQD QNLI DARSKLNTTPE SSGTI+TATS
Sbjct: 781 VCLNSVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 818

BLAST of HG10000313 vs. ExPASy TrEMBL
Match: A0A6J1L1A5 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499516 PE=3 SV=1)

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 739/827 (89.36%), Postives = 771/827 (93.23%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL +PI+ S+P   LLTR TNA A+ RIGPVS FS RSS IRL R             
Sbjct: 1   MDTLFIPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSAIRLKR------------- 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
             FRCAA+ASD RH HH      HHH HH CQHH  GDGDGVELTGAQKAFV FA+AIRW
Sbjct: 61  --FRCAADASDHRHGHH------HHHHHHSCQHHCGGDGDGVELTGAQKAFVGFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLHLCCGSAALF+TAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATIT
Sbjct: 241 SHPDFALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQ NKPKL
Sbjct: 301 VEHLTGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQQNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD+SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFP
Sbjct: 481 IGGDKSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIK+AVR S++GSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSFGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPR  V+DAIAEL+D+ KLRVMMLTGDHDSSAWK+ANAVGI+EVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGISEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNSIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 VCLNSIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 806

BLAST of HG10000313 vs. ExPASy TrEMBL
Match: A0A6J1G6Z6 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451327 PE=3 SV=1)

HSP 1 Score: 1420.2 bits (3675), Expect = 0.0e+00
Identity = 739/827 (89.36%), Postives = 769/827 (92.99%), Query Frame = 0

Query: 53  MDTLVVPISLSEPPRPLLTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRF 112
           MDTL VPI+ S+P   LLTR TNA A+ RIGPVS FS RSS+IRL R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 113 RHFRCAAEASDRRHSHHHGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRW 172
             FRCAA+ASD        H HGHHH HH CQHH  GDGDGVELTGAQKAFVRFA+AIRW
Sbjct: 61  --FRCAADASD--------HRHGHHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAEAIRW 120

Query: 173 TDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDAL 232
           TDLANYLREHLHLCCGSAALF+TAAACPYLVPKPAVK LQNVFIAVAFPLVGVSASLDAL
Sbjct: 121 TDLANYLREHLHLCCGSAALFITAAACPYLVPKPAVKLLQNVFIAVAFPLVGVSASLDAL 180

Query: 233 TDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKE 292
           TD+SGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKE
Sbjct: 181 TDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKE 240

Query: 293 SHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATIT 352
           SHPDFALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATIT
Sbjct: 241 SHPDFALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATIT 300

Query: 353 VEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKL 412
           VEHLTGE+KPLQIKVG+RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKL
Sbjct: 301 VEHLTGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKL 360

Query: 413 QRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA 472
           QRWL+EFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALA
Sbjct: 361 QRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALA 420

Query: 473 AAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHK 532
           AAPLAYAIAISSCARKGILLKGGHVLDAMASC TVAFDKTGTLTTGGLVFKAIEPIYGHK
Sbjct: 421 AAPLAYAIAISSCARKGILLKGGHVLDAMASCDTVAFDKTGTLTTGGLVFKAIEPIYGHK 480

Query: 533 IGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFP 592
           IGGD SH ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISV+SFEYFP
Sbjct: 481 IGGDNSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFP 540

Query: 593 GRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAA 652
           GRGLIATLHGIKSGIGG LRKASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAA
Sbjct: 541 GRGLIATLHGIKSGIGGPLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAA 600

Query: 653 LSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYY 712
           LSVDQKVTLIHLEDQPR  V+DAIAEL+D+ KLRVMMLTGDHDSSAWK+ANAVGI+EVYY
Sbjct: 601 LSVDQKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGIDEVYY 660

Query: 713 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLL 772
           SLKPEDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLL
Sbjct: 661 SLKPEDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLL 720

Query: 773 LQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLL 832
           LQDSISGVPFCIAKSRQTT+LVKQNVTLALSSILLASLPS+LGFLPLWLTVLLHEGGTLL
Sbjct: 721 LQDSISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLL 780

Query: 833 VCLNSIRALNQPSWSWKQDFQNLICDARSKLNTTPETSSGTIRTATS 880
           VCLNSIRALN PSWSWKQDFQNLI +ARS L+TTPE SSGTIRTA+S
Sbjct: 781 VCLNSIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 804

BLAST of HG10000313 vs. ExPASy TrEMBL
Match: A0A6J1DBA2 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018894 PE=3 SV=1)

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 717/809 (88.63%), Postives = 751/809 (92.83%), Query Frame = 0

Query: 70  LTRNTNAVAQWRIGPVSRFSPRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHH 129
           + R TN  A     PV RFS RSSLI +    +  K   YPR R   CA E +       
Sbjct: 11  IRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRLRRIPCAPEGA------- 70

Query: 130 HGHHHGHHHGHHGCQHHSSGDGDGVELTGAQKAFVRFADAIRWTDLANYLREHLHLCCGS 189
            GHHHGH   HHGCQHH   DGDGVE TGAQKAF+RFA AIRWT+LANYLREHL LCCGS
Sbjct: 71  -GHHHGH---HHGCQHHC--DGDGVEPTGAQKAFIRFAKAIRWTELANYLREHLQLCCGS 130

Query: 190 AALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALA 249
           AALF+TAAACPYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTD+SGGKVNIHVLMALA
Sbjct: 131 AALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDISGGKVNIHVLMALA 190

Query: 250 AFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLP 309
           AFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPDFALVLD+NDDNLP
Sbjct: 191 AFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPDFALVLDMNDDNLP 250

Query: 310 DTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGE 369
           DTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPL+IKVGE
Sbjct: 251 DTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLEIKVGE 310

Query: 370 RVPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVV 429
           RVPGGARNLDGRIIVKATK WEESTLSRIVQLTEEAQLNKPKLQRWL+EFGENYSKVVVV
Sbjct: 311 RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVV 370

Query: 430 LSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKG 489
           LSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKG
Sbjct: 371 LSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKG 430

Query: 490 ILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCE 549
           ILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+++H ASCCIPSCE
Sbjct: 431 ILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGEKTHLASCCIPSCE 490

Query: 550 KEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGG 609
           KEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISV+SFEYFPGRGLIATLHGIKSGIGG
Sbjct: 491 KEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGLIATLHGIKSGIGG 550

Query: 610 KLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPR 669
           KLRKASLGSVDFITSFCKSENESRKIK+AVR S+YGSEFVHAALSVDQKVTLIHLEDQPR
Sbjct: 551 KLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVDQKVTLIHLEDQPR 610

Query: 670 PEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISRE 729
           P +++AI+EL+D  K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISRE
Sbjct: 611 PGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISRE 670

Query: 730 MGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQ 789
           MGGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+IS VPFCIAKSRQ
Sbjct: 671 MGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDNISCVPFCIAKSRQ 730

Query: 790 TTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWK 849
           TTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWK
Sbjct: 731 TTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWK 790

Query: 850 QDFQNLICDARSKLNTTPETSSGTIRTAT 879
           QDFQNL+ DARS++  T +TSSGTI+TA+
Sbjct: 791 QDFQNLVRDARSRVLATRQTSSGTIQTAS 806

BLAST of HG10000313 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 574/802 (71.57%), Postives = 653/802 (81.42%), Query Frame = 0

Query: 81  RIGPVSRFS--PRSSLIRLNRFPICYKKLSYPRFRHFRCAAEASDRRHSHHHG-----HH 140
           R+  V+ FS  P  +L+R     I       PR    R      D  H HHH      H+
Sbjct: 25  RLARVNSFSILPPKTLLRQKPLRISASLNLPPRSIRLRA---VEDHHHDHHHDDEQDHHN 84

Query: 141 HGHHHGHHGCQHHSSGDGDGVEL---TGAQKAFVRFADAIRWTDLANYLREHLHLCCGSA 200
           H HHH  HGC          VEL   +  QK    FA AI W  LANYLREHLHLCC +A
Sbjct: 85  HHHHHHQHGC--------CSVELKAESKPQKMLFGFAKAIGWVRLANYLREHLHLCCSAA 144

Query: 201 ALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDLSGGKVNIHVLMALAA 260
           A+F+ AA CPYL P+P +K LQN F+ V FPLVGVSASLDAL D++GGKVNIHVLMALAA
Sbjct: 145 AMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVNIHVLMALAA 204

Query: 261 FASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPD 320
           FAS+FMGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL+++V++ N+P+
Sbjct: 205 FASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLIEVHNGNVPN 264

Query: 321 TSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGER 380
            SDL ++ VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVKPL+ K G+R
Sbjct: 265 ISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVKPLEAKAGDR 324

Query: 381 VPGGARNLDGRIIVKATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVL 440
           VPGGARNLDGR+IVKATKAW +STL++IVQLTEEA  NKPKLQRWL+EFGENYSKVVVVL
Sbjct: 325 VPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGENYSKVVVVL 384

Query: 441 SVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIAISSCARKGI 500
           S+A+A +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA AISSCARKGI
Sbjct: 385 SLAIAFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATAISSCARKGI 444

Query: 501 LLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEK 560
           LLKG  VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   +CCIP+CEK
Sbjct: 445 LLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVITCCIPNCEK 504

Query: 561 EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKS-GIGG 620
           EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSI V+SFEYFPGRGL AT++G+K+     
Sbjct: 505 EALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVNGVKTVAEES 564

Query: 621 KLRKASLGSVDFITSFCKSENESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPR 680
           +LRKASLGS++FITS  KSE+ES++IKDAV  S+YG +FVHAALSVDQKVTLIHLEDQPR
Sbjct: 565 RLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVTLIHLEDQPR 624

Query: 681 PEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKLAHVKEISRE 740
           P V   IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL HVK I+RE
Sbjct: 625 PGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKLNHVKNIARE 684

Query: 741 MGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQ 800
            GGGLIMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GVPFC+AKSRQ
Sbjct: 685 AGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGVPFCVAKSRQ 744

Query: 801 TTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWK 860
           TTSLVKQNV LAL+SI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNS+R LN PSWSWK
Sbjct: 745 TTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRGLNDPSWSWK 804

Query: 861 QDFQNLICDARSKLNTTPETSS 872
           QD  +LI   RS+  T+  ++S
Sbjct: 805 QDIVHLINKLRSQEPTSSSSNS 814

BLAST of HG10000313 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 205.7 bits (522), Expect = 1.5e-52
Identity = 180/660 (27.27%), Postives = 316/660 (47.88%), Query Frame = 0

Query: 215 FIAVAFPLVGVSASL-DALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 274
           ++AVA    G+   L  A   +   +++I++L+ +   A++ M + +E   ++ +F ++ 
Sbjct: 122 WLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISD 181

Query: 275 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 334
             E   + ++   ++ L    P  A++ +  ++            V V +V+V + + V 
Sbjct: 182 WLETRASYKATSVMQSLMSLAPQKAIIAETGEE------------VEVDEVKVDTVVAVK 241

Query: 335 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 394
           AGE++P+D  V  G   +  + LTGE  P+  +    V  G  NL+G I VK T    + 
Sbjct: 242 AGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDC 301

Query: 395 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 454
            ++++ +L EEAQ +K K QR +++  + Y+  ++++S  VA++ P++ K         +
Sbjct: 302 VVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK-----VHNLK 361

Query: 455 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 514
              + AL ++V+  PC L  + P+A   A++  A  G+L+K    LD ++    VAFDKT
Sbjct: 362 HWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKT 421

Query: 515 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 574
           GT+T G  +                  F S       +  L   +++E  ++HP+   +V
Sbjct: 422 GTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIV 481

Query: 575 DH--SVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 634
           D+  SV  +     V+ ++ FPG G+   + G    IG K   +  G        C +  
Sbjct: 482 DYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAG--------CSTVP 541

Query: 635 ESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMML 694
           E       +   T G + V      ++     +L D  R  V  A+AEL+  G ++  ML
Sbjct: 542 E-------IEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLG-IKTAML 601

Query: 695 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 754
           TGD+ ++A      +G  ++ V+  L PEDK   ++E  +E  G   MVG+G+NDAPALA
Sbjct: 602 TGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGVNDAPALA 661

Query: 755 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLA 814
            A +GI +    SA AT   +++L+ + I  +P  +  +R+    V +NV   LS IL A
Sbjct: 662 TADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENV--CLSIILKA 721

Query: 815 SLPSV--LGFLPLWLTVLLHEGGTLLVCLNSIRALNQPSWSWKQDFQNLIC--DARSKLN 865
            + ++   G   +W  VL+  G  LLV  NS+  L +     K+   N  C   + SKLN
Sbjct: 722 GILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLRE-----KKKIGNKKCYRASTSKLN 723

BLAST of HG10000313 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 204.5 bits (519), Expect = 3.4e-52
Identity = 172/634 (27.13%), Postives = 307/634 (48.42%), Query Frame = 0

Query: 215 FIAVAFPLVGVSASL-DALTDLSGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 274
           ++AVA  + G+   L  A+  L+  +++I++L+ +   A+I M +  E  +++ +F +A 
Sbjct: 112 WLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAE 171

Query: 275 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 334
             +   + ++   ++ L    P  A++ +  ++            V V +++  + I V 
Sbjct: 172 WLQSRASYKASAVMQSLMSLAPQKAVIAETGEE------------VEVDELKTNTVIAVK 231

Query: 335 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKAWEES 394
           AGE++P+D  V  G   +  + LTGE  P+       V  G  NL+G I V  T   E+ 
Sbjct: 232 AGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDC 291

Query: 395 TLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 454
            ++++ +L EEAQ +K + QR++++  + Y+  ++++S+    I P   K         +
Sbjct: 292 VVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-PFALK-----VHNLK 351

Query: 455 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 514
             V+ AL ++V+A PC L  + P+A   A++  A  G+L+KG   L+ +A    VAFDKT
Sbjct: 352 HWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKT 411

Query: 515 GTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 574
           GT+T G  +                  F S       +  L   ++ E  ++HP+  AVV
Sbjct: 412 GTITRGEFIV---------------MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVV 471

Query: 575 DH--SVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKLRKASLGSVDFITSFCKSEN 634
           D+  SV  +    +V+ ++ FPG G+   + G +  IG K   +  G        C S  
Sbjct: 472 DYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAG--------CLSVP 531

Query: 635 ESRKIKDAVRTSTYGSEFVHAALSVDQKVTLIHLEDQPRPEVVDAIAELRDKGKLRVMML 694
           +       +   T G + +      +    + +L D  R  V  A+ EL+  G +++ ML
Sbjct: 532 D-------IDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLG-IKIAML 591

Query: 695 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 754
           TGD+ ++A      +G  ++ V   L PEDK   +K++ RE  G   MVG+G+NDAPALA
Sbjct: 592 TGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE-EGPTAMVGDGLNDAPALA 651

Query: 755 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLVKQNVTLALSSILLA 814
            A +GI +    SA AT   +++L+ + I  +P  I  +++    V +NV ++++     
Sbjct: 652 TADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAI 695

Query: 815 SLPSVLGFLPLWLTVLLHEGGTLLVCLNSIRALN 843
              +  G   +W  VL   G  LLV LNS+  L+
Sbjct: 712 LALAFAGHPLIWAAVLADVGTCLLVILNSMLLLS 695

BLAST of HG10000313 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.7 bits (491), Expect = 6.1e-49
Identity = 200/700 (28.57%), Postives = 319/700 (45.57%), Query Frame = 0

Query: 169 AIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFI---AVAFPLVGV 228
           A  WT +A        LCCGS    +  +   ++        L N ++        L+G 
Sbjct: 180 AFAWTLVA--------LCCGSHTSHILHSLGIHIAHGGIWDLLHNSYVKGGLAVGALLGP 239

Query: 229 SASL--DALTDLSGGKVNIHVLMALAAFAS-----IFMGNPL---------EGGLLLVMF 288
              L  D +        N++ L+ L + A+     I + NP          E  +LL   
Sbjct: 240 GRELLFDGIKAFGKRSPNMNSLVGLGSMAAFSISLISLVNPELEWDASFFDEPVMLLGFV 299

Query: 289 NMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----RVPVRDV 348
            +    EE    Q+  D+ EL         LV+  +D+N P  S L        V V D+
Sbjct: 300 LLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVSVDDI 359

Query: 349 EVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIV 408
            VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N DG + +
Sbjct: 360 RVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDGPLRI 419

Query: 409 KATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAV----ALIGPL 468
           KA+     ST+S+IV++ E+AQ N   +QR  +     +   ++ LS         +G  
Sbjct: 420 KASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYYVGSH 479

Query: 469 LFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGIL 528
           +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  A++G L
Sbjct: 480 IF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKRGYL 539

Query: 529 LKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKE 588
           ++GG VL+ +AS   VA DKTGTLT G  V   +  + G++                E+E
Sbjct: 540 IRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE----------------EQE 599

Query: 589 ALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKL 648
            L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G         
Sbjct: 600 VLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--------- 659

Query: 649 RKASLGSVDFITSFCKSENES-----------RKIKDAVRTSTYGSEFVHAALSVDQKVT 708
           R  ++GS+++++     +N+S            K+ +   TS Y    V+     +  + 
Sbjct: 660 RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIG 719

Query: 709 LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGI--NEVYYSLKPED 768
            I + D  R +    +A L++KG ++ ++L+GD + +   VA  VGI      YSL PE 
Sbjct: 720 AIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNYSLSPEK 779

Query: 769 KLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVLLLQDSI 818
           K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+L+++ +
Sbjct: 780 KFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVILVRNKL 839

BLAST of HG10000313 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 193.7 bits (491), Expect = 6.1e-49
Identity = 200/700 (28.57%), Postives = 319/700 (45.57%), Query Frame = 0

Query: 169 AIRWTDLANYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFI---AVAFPLVGV 228
           A  WT +A        LCCGS    +  +   ++        L N ++        L+G 
Sbjct: 180 AFAWTLVA--------LCCGSHTSHILHSLGIHIAHGGIWDLLHNSYVKGGLAVGALLGP 239

Query: 229 SASL--DALTDLSGGKVNIHVLMALAAFAS-----IFMGNPL---------EGGLLLVMF 288
              L  D +        N++ L+ L + A+     I + NP          E  +LL   
Sbjct: 240 GRELLFDGIKAFGKRSPNMNSLVGLGSMAAFSISLISLVNPELEWDASFFDEPVMLLGFV 299

Query: 289 NMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----RVPVRDV 348
            +    EE    Q+  D+ EL         LV+  +D+N P  S L        V V D+
Sbjct: 300 LLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICINVSVDDI 359

Query: 349 EVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIV 408
            VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N DG + +
Sbjct: 360 RVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINWDGPLRI 419

Query: 409 KATKAWEESTLSRIVQLTEEAQLNKPKLQRWLNEFGENYSKVVVVLSVAV----ALIGPL 468
           KA+     ST+S+IV++ E+AQ N   +QR  +     +   ++ LS         +G  
Sbjct: 420 KASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFWYYVGSH 479

Query: 469 LFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSCARKGIL 528
           +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  A++G L
Sbjct: 480 IF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLGAKRGYL 539

Query: 529 LKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDESHFASCCIPSCEKE 588
           ++GG VL+ +AS   VA DKTGTLT G  V   +  + G++                E+E
Sbjct: 540 IRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE----------------EQE 599

Query: 589 ALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVDSFEYFPGRGLIATLHGIKSGIGGKL 648
            L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G         
Sbjct: 600 VLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--------- 659

Query: 649 RKASLGSVDFITSFCKSENES-----------RKIKDAVRTSTYGSEFVHAALSVDQKVT 708
           R  ++GS+++++     +N+S            K+ +   TS Y    V+     +  + 
Sbjct: 660 RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGREGEGIIG 719

Query: 709 LIHLEDQPRPEVVDAIAELRDKGKLRVMMLTGDHDSSAWKVANAVGI--NEVYYSLKPED 768
            I + D  R +    +A L++KG ++ ++L+GD + +   VA  VGI      YSL PE 
Sbjct: 720 AIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNYSLSPEK 779

Query: 769 KLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVLLLQDSI 818
           K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+L+++ +
Sbjct: 780 KFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVILVRNKL 839