HG10000146 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000146
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionprotein NRT1/ PTR FAMILY 6.4
LocationChr09: 1468596 .. 1471342 (+)
RNA-Seq ExpressionHG10000146
SyntenyHG10000146
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAACTTGTTTTTGGTGAAACTCCTTAGTTTCTATTGGCTATGATATGGCTTTTTTAACAGTTAAATTTTTCCTTTTTATCAACAGGTTCTGGTATCAAAACATGGGAGTGGAGGAGAAGAAGATGATGCTGCAGTAGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACAGGGGGATGGCTTGCAGCAGGGCTCATCTTAGGTGCTTAAATTTTTCTTTGGTTTTCACAGAACAAAATTCTAACTTTATCGGTGTAATGAACTGATAAGTAACAATTCTAAATGCCCAAAATCATGCAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTTGTTGGGGATTTGCATCTCACTTCAGCCAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCTCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGCTGGGAAGGTACCTCACTGTTGCAACCTTTGCTTCCATAACTGCTGTGGTAAGTTTTGAGCCATAAAGAAATGACTTTCAACAAAGAAAATTTTACTTCAGAATTTAGCCTGAGCCTTCCTGGTTTTTGCAGGGTGTTATTTTGTTAACATTGGCCACAACTATCCCTGGCATGAGACCCCCTCATTGTGATGACTCCACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCGGGATTTGGGTCCGATCAATTCGACACTAATGATCCGAAGGAGGAGAAGTCCATGATATTCTTCTTCAATAGGTTCTACTTTGCAATAAGCATCGGGTCGTTGTTTGCCGTCGTTGTGTTGGTTTATGTACAAGACAATGTGGGGAGAGGATGGGGATATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTTCTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGCCCTCTAACTGTGATATGGAGAGTAGTGCTTTTGGCTTGGAAGAAGAGAAGTCTACCTCACCCAGCTCACCCCAGCTACTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAGTAAGTGTTTTTCAGATCTATCATCAAATACTCTTTGCTTTTTCTATCCCTCACTCTTTTGCTTTCTTTAAGTGTTTGGATTGTTGAGTCGAGTTATGAAGTTTAGAGTTCAAATGTTGGAATTAGGAAGTCGGTATTTAAGATGCAAAGTTGTTCGATAAAATTTTTAGATAAATGTGCAAAATAGAGAAAGAGGAAAAGATGAAGTTCTCAATAAACGTAAAAACTTCAATAATAAACACTATTAAAGTTTAGTTATTTAACACTAACTTATGATGTTGATGGATCAAACACCCTCTTACAGTTAGAAAATAGAAGATTTAAACCTACAACCATAAAGAGAAGTATTAATGATCTAGTCGAGCAAATTATGTGTGCGCTTATCTCATTTTTTCTTCATTGCTTCATTTCCTCCAACCATAAAGAGATGTATAAATTGTTAGCCATATCTAGGAATAGTCGACCAGGAACATTCACAATGTTTTAATTTACATCTGTTGCTTCGGTTTAATGATTACCTGTTTTTTGTTTTAAATGTAATATAAGATACACTGCAAGATGCCATTGTATAGAAACAAAAAGAATAAAACATTACTATTCATGCTTGTCCTGTTCAAACCATTCCACATATAAATTGGTGTATTCAATTCACTTGATTCATTTGGTTATTTGCAAGAACATAGAGTAATGAAAATGTGAATATTAGGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGCTGCCACTGATCCAAACAGATATGATCCATGGATAGTCTCAACAGTGACTGAAATTGAAGAAGTGAAAATGGTGTTGAAGCTTTTACCCATTTGGTCCACTGGCATCCTCTTCTGGACGATCTACTCTCAGATGACTACATTCACAGTAGAGCAAGCATCATTTATGCAAAGAAAAGTTGGCGGTTTTGAGATTCCTCCCGGTTCTATGTCAGCCTTTCTGTTCATCGCCATCCTGCTCGTCACTTCCATGAATGAGAAACTATTCATACCGATTGCCCGGAAGTTGACTCACAATGTCCAAGGTCTCACAAGCCTTCAGAGGATTGGGATTGGACTCCTTTTCTCAGTTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAACGAGAGGAAGGCATTTGCTGTGGGGCAAAATACTCGCATAAGTGCTTTCTGGTTGATACCTCAATTCTTCTTGGTTGGTGCTGGAGAAGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGACTTTTTCTTAGCACTCTTTCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCACTGGCTGAGAAGTAATCTCAACAAGGGAAACTTGAACTACTTCTATTGGCTACTCGCAGTGCTAGGCCTGCTAAACTTTTTCTTTTTTCTTCTCCTTGCCCGAAAACATCAGTATAAAGACCAACATTATGTCAGCTCCAAAGACAACGAGGAGCTCAAGACTTCAAATGACATGGTCGCACTTGAAATCGACGGGCCAATAAGCATGAAGGCAACAGAAACTTAG

mRNA sequence

ATGGTTCTGGTATCAAAACATGGGAGTGGAGGAGAAGAAGATGATGCTGCAGTAGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACAGGGGGATGGCTTGCAGCAGGGCTCATCTTAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTTGTTGGGGATTTGCATCTCACTTCAGCCAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCTCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGCTGGGAAGGTACCTCACTGTTGCAACCTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTAACATTGGCCACAACTATCCCTGGCATGAGACCCCCTCATTGTGATGACTCCACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCGGGATTTGGGTCCGATCAATTCGACACTAATGATCCGAAGGAGGAGAAGTCCATGATATTCTTCTTCAATAGGTTCTACTTTGCAATAAGCATCGGGTCGTTGTTTGCCGTCGTTGTGTTGGTTTATGTACAAGACAATGTGGGGAGAGGATGGGGATATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTTCTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGCCCTCTAACTGTGATATGGAGAGTAGTGCTTTTGGCTTGGAAGAAGAGAAGTCTACCTCACCCAGCTCACCCCAGCTACTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGCTGCCACTGATCCAAACAGATATGATCCATGGATAGTCTCAACAGTGACTGAAATTGAAGAAGTGAAAATGGTGTTGAAGCTTTTACCCATTTGGTCCACTGGCATCCTCTTCTGGACGATCTACTCTCAGATGACTACATTCACAGTAGAGCAAGCATCATTTATGCAAAGAAAAGTTGGCGGTTTTGAGATTCCTCCCGGTTCTATGTCAGCCTTTCTGTTCATCGCCATCCTGCTCGTCACTTCCATGAATGAGAAACTATTCATACCGATTGCCCGGAAGTTGACTCACAATGTCCAAGGTCTCACAAGCCTTCAGAGGATTGGGATTGGACTCCTTTTCTCAGTTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAACGAGAGGAAGGCATTTGCTGTGGGGCAAAATACTCGCATAAGTGCTTTCTGGTTGATACCTCAATTCTTCTTGGTTGGTGCTGGAGAAGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGACTTTTTCTTAGCACTCTTTCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCACTGGCTGAGAAGTAATCTCAACAAGGGAAACTTGAACTACTTCTATTGGCTACTCGCAGTGCTAGGCCTGCTAAACTTTTTCTTTTTTCTTCTCCTTGCCCGAAAACATCAGTATAAAGACCAACATTATGTCAGCTCCAAAGACAACGAGGAGCTCAAGACTTCAAATGACATGGTCGCACTTGAAATCGACGGGCCAATAAGCATGAAGGCAACAGAAACTTAG

Coding sequence (CDS)

ATGGTTCTGGTATCAAAACATGGGAGTGGAGGAGAAGAAGATGATGCTGCAGTAGATTTTTGGGGAAACCCAGTGGACAAATCAAAAACAGGGGGATGGCTTGCAGCAGGGCTCATCTTAGGAACTGAACTCTCTGAAAGGATATGTGTGATGGGAATATCAATGAACTTAGTGACATATCTTGTTGGGGATTTGCATCTCACTTCAGCCAAATCTGCAACTATTGTGACTAATTTTCTTGGTGCTCTTAATCTTCTTGGCCTTCTTGGTGGGTTCTTAGCAGATGCCAAGCTGGGAAGGTACCTCACTGTTGCAACCTTTGCTTCCATAACTGCTGTGGGTGTTATTTTGTTAACATTGGCCACAACTATCCCTGGCATGAGACCCCCTCATTGTGATGACTCCACAAGGCAACTTCATCAATGCATTGAAGCTAATGGTGGGCAACTTGCCATGCTCTATGCTGCACTTTATACTATAGCACTCGGCGGAGGGGGGATAAAATCAAACGTTTCGGGATTTGGGTCCGATCAATTCGACACTAATGATCCGAAGGAGGAGAAGTCCATGATATTCTTCTTCAATAGGTTCTACTTTGCAATAAGCATCGGGTCGTTGTTTGCCGTCGTTGTGTTGGTTTATGTACAAGACAATGTGGGGAGAGGATGGGGATATGGAATCTCAGGAGGGACCATGGTGATTGCTGTAATTGTTCTGCTTTGTGGGACAACCTTTTACAGGTTTAAGAAGCCTAGAGGAAGCCCTCTAACTGTGATATGGAGAGTAGTGCTTTTGGCTTGGAAGAAGAGAAGTCTACCTCACCCAGCTCACCCCAGCTACTTAAATGACTACCAAAATGCCAAGGTTCCCTACACCTACAGGTTCAAGTGTCTTGACAAGGCTGCAATTCTGGACGAGTATGCTGCCACTGATCCAAACAGATATGATCCATGGATAGTCTCAACAGTGACTGAAATTGAAGAAGTGAAAATGGTGTTGAAGCTTTTACCCATTTGGTCCACTGGCATCCTCTTCTGGACGATCTACTCTCAGATGACTACATTCACAGTAGAGCAAGCATCATTTATGCAAAGAAAAGTTGGCGGTTTTGAGATTCCTCCCGGTTCTATGTCAGCCTTTCTGTTCATCGCCATCCTGCTCGTCACTTCCATGAATGAGAAACTATTCATACCGATTGCCCGGAAGTTGACTCACAATGTCCAAGGTCTCACAAGCCTTCAGAGGATTGGGATTGGACTCCTTTTCTCAGTTTTTGGCATGGTGGCTGCTGGAGTTGTTGAGAACGAGAGGAAGGCATTTGCTGTGGGGCAAAATACTCGCATAAGTGCTTTCTGGTTGATACCTCAATTCTTCTTGGTTGGTGCTGGAGAAGCATTTGCATATGTTGGACAACTCGAGTTTTTCATCAGAGAAGCACCGGAACGAATGAAATCCATGAGCACAGGACTTTTTCTTAGCACTCTTTCCATGGGATTTTTTGTTAGTAGTTTGTTGGTAACAATTGTAGATAAAGTAACCAACAAGCACTGGCTGAGAAGTAATCTCAACAAGGGAAACTTGAACTACTTCTATTGGCTACTCGCAGTGCTAGGCCTGCTAAACTTTTTCTTTTTTCTTCTCCTTGCCCGAAAACATCAGTATAAAGACCAACATTATGTCAGCTCCAAAGACAACGAGGAGCTCAAGACTTCAAATGACATGGTCGCACTTGAAATCGACGGGCCAATAAGCATGAAGGCAACAGAAACTTAG

Protein sequence

MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDKAAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKDNEELKTSNDMVALEIDGPISMKATET
Homology
BLAST of HG10000146 vs. NCBI nr
Match: XP_008455932.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 560/591 (94.75%), Postives = 577/591 (97.63%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEYA TDPN  +PWIVSTVTEIEEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATTDPN--NPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R+VG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELK SNDMVALEI+GPISMKA ET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of HG10000146 vs. NCBI nr
Match: KAA0062862.1 (protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 558/591 (94.42%), Postives = 577/591 (97.63%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVG+LHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEYA TDPN  +PWIVSTVTEIEEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATTDPN--NPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R+VG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPISMKA ET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAET 589

BLAST of HG10000146 vs. NCBI nr
Match: XP_011649985.1 (protein NRT1/ PTR FAMILY 6.4 [Cucumis sativus] >KGN63217.1 hypothetical protein Csa_022407 [Cucumis sativus])

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 554/591 (93.74%), Postives = 572/591 (96.79%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPK EK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYA T PN  +PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYATTGPN--NPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+RKVG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFF+LLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPIS KA ET
Sbjct: 541 NFFFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of HG10000146 vs. NCBI nr
Match: AFR11353.1 (nitrate transporter [Cucumis sativus])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 553/591 (93.57%), Postives = 571/591 (96.62%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPK EK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYA T PN  +PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYATTGPN--NPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+RKVG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFF+LLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPIS KA ET
Sbjct: 541 NFFFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of HG10000146 vs. NCBI nr
Match: XP_038901295.1 (protein NRT1/ PTR FAMILY 6.4 [Benincasa hispida])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 552/591 (93.40%), Postives = 569/591 (96.28%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSK GGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKRGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA+FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVASFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPP CD STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPQCDYSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLF+V VLVYVQD+VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFSVTVLVYVQDHVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGT FYRFKKPRGSPLTVIWRVVLLAWKKRSLPH AHPS+LNDYQNAKVPYTY+FKCLDK
Sbjct: 241 CGTYFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHAAHPSFLNDYQNAKVPYTYKFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYA TDPN  +PWIVSTVTEIEEVKMVLKL+PIWSTGILFWTIYSQMTTFTV QA
Sbjct: 301 AAILDEYAVTDPN--NPWIVSTVTEIEEVKMVLKLIPIWSTGILFWTIYSQMTTFTVVQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R VG FEIP GSMSAFLFI ILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERNVGSFEIPAGSMSAFLFITILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
            FS+FGMVAAGVVE ERK FAV QNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 FFSIFGMVAAGVVEKERKTFAVEQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLR+NLNKG+LNYFYWLLAVLGLL
Sbjct: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRNNLNKGDLNYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELKTSNDM+ALE++G IS+KATET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKTSNDMIALEMEGSISIKATET 589

BLAST of HG10000146 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 2.0e-234
Identity = 408/558 (73.12%), Postives = 463/558 (82.97%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MV VS      +  + A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTY
Sbjct: 1   MVHVSSSHGAKDGSEEAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLH++SAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+TA+GV+LLT+
Sbjct: 61  LVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTV 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTI  MRPP CDD  R  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           T+DPKEEK MIFFFNRFYF+IS+GSLFAV+ LVYVQDNVGRGWGYGIS  TMV+A IVLL
Sbjct: 181 TSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGT  YRFKKP+GSP T IWRV  LAWKKR   +PAHPS LN Y N  VP+T   KCLDK
Sbjct: 241 CGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDK 300

Query: 301 AAIL---DEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTV 360
           AAI       ++ D    DPWIVSTVT++EEVK+V+KL+PIW+T ILFWTIYSQMTTFTV
Sbjct: 301 AAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTV 360

Query: 361 EQASFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIG 420
           EQA+FM RK+G F +P GS SAFL + ILL TS+NE++F+P+ R+LT   QG+TSLQRIG
Sbjct: 361 EQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIG 420

Query: 421 IGLLFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIR 480
           +GL+FS+  M  A V+EN R+  AV  + +ISAFWL+PQ+FLVGAGEAFAYVGQLEFFIR
Sbjct: 421 VGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIR 480

Query: 481 EAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVL 540
           EAPERMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLNK  LNYFYWLL VL
Sbjct: 481 EAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVL 540

Query: 541 GLLNFFFFLLLARKHQYK 556
           G LNF  F++ A KHQYK
Sbjct: 541 GALNFLIFIVFAMKHQYK 558

BLAST of HG10000146 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 3.9e-153
Identity = 287/567 (50.62%), Postives = 386/567 (68.08%), Query Frame = 0

Query: 12  EEDDAAVDFW---GNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLT 71
           + DD  +D W   G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL 
Sbjct: 7   KSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLG 66

Query: 72  SAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMR 131
           +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+A FA+I A GV +LTL+T IPG+R
Sbjct: 67  NATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLR 126

Query: 132 PPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEK 191
           PP C+ +T     C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE  
Sbjct: 127 PPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 186

Query: 192 SMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRF 251
            M +FFNRF+F I++GSL AV VLVYVQD+VGR WGYGI    +V+A+ V L GT  YRF
Sbjct: 187 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 246

Query: 252 KKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQN-----------AKVPYTYRFKC 311
           KK  GSP+T +  V++ AW+ R L  PA PSYL D  +            K+P+T +F+ 
Sbjct: 247 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 306

Query: 312 LDKAAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTV 371
           LDKAAI D+ A    N ++ W +ST+T++EEVK ++++LPIW+T ILFWT+++Q+TT +V
Sbjct: 307 LDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSV 366

Query: 372 EQASFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIG 431
            Q+  + R +G FEIPP SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG
Sbjct: 367 AQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIG 426

Query: 432 IGLLFSVFGMVAAGVVENE--RKAFAVGQNTR---ISAFWLIPQFFLVGAGEAFAYVGQL 491
           +GL F    M  A +VE +  R A A G   +   +  + LIPQ+ +VG GEA  Y GQL
Sbjct: 427 LGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQL 486

Query: 492 EFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--HWLRSNLNKGNLNYF 551
           +FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LNKG L  F
Sbjct: 487 DFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNF 546

Query: 552 YWLLAVLGLLNFFFFLLLARKHQYKDQ 558
           YWL+AVL  LNF  FL+ ++ + YK++
Sbjct: 547 YWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of HG10000146 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.9e-147
Identity = 281/549 (51.18%), Postives = 381/549 (69.40%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDST 136
           T+F+G   LL LLGGFLAD+ LGR+ T+  F++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+  M FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD VGR W YGI   +M IA+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVVLLAWKKRSLPHPAHPSYL--NDYQNAKVPYTYRFKCLDKAAILDE-------Y 316
             I++V+  A++KR +  P    YL  ++ +  ++ +T +F  LDKAAI+ E        
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 AATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRKV 376
               PN   PW +S+VT++EEVKM+++LLPIW+T I+FWT Y+QM TF+VEQAS M+R +
Sbjct: 312 GVAIPN---PWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNI 371

Query: 377 GGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVFGM 436
           G F+IP GS++ F   AIL+  ++ ++  +P  +K      G +SLQRI IGL+ S  GM
Sbjct: 372 GSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGM 431

Query: 437 VAAGVVENERKAFAVGQNTR---ISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMK 496
            AA +VE +R + A   + +   IS F L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK
Sbjct: 432 AAAALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMK 491

Query: 497 SMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKH----WLRSNLNKGNLNYFYWLLAVLGLL 550
           +MSTGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L+YFYWLL +L  +
Sbjct: 492 TMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGI 551

BLAST of HG10000146 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 6.4e-124
Identity = 241/560 (43.04%), Postives = 353/560 (63.04%), Query Frame = 0

Query: 15  DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A 
Sbjct: 10  DGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAAN 69

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDD 134
            VTN+ G   +  L+G F+ADA LGRY T+ATF  I   G+ LLTL+ ++PG++P +C+ 
Sbjct: 70  NVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNA 129

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFF 194
            T         N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     FF
Sbjct: 130 DT------CHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 189

Query: 195 NRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGS 254
           N FYF+I++G+L A  VLV++Q NVG GWG+G+    MVIAV     G+ FYR ++P GS
Sbjct: 190 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 249

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSYL--------NDYQNAKVPYTYRFKCLDKAAILDE 314
           PLT I++V++ A++K S+  P   S L        N   + K+ +T   K  DKAA+  +
Sbjct: 250 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 309

Query: 315 YAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRK 374
             +      +PW + +VT++EE+K ++ LLP+W+TGI+F T+YSQM+T  V Q + M + 
Sbjct: 310 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 369

Query: 375 VG-GFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVF 434
           +G  FEIP  S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGL+ S+F
Sbjct: 370 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIF 429

Query: 435 GMVAAGVVENERKAFAVGQNT------RISAFWLIPQFFLVGAGEAFAYVGQLEFFIREA 494
            M+ AGV+E  R  +    N        +S FW IPQ+ L+G  E F ++GQLEFF  +A
Sbjct: 430 AMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQA 489

Query: 495 PERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKH----WLRSNLNKGNLNYFYWLLA 554
           P+ M+S+ + L L+T+++G ++S++LVT+V K+T K+    W+  NLN+G+L+YF++LLA
Sbjct: 490 PDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLA 549

Query: 555 VLGLLNFFFFLLLARKHQYK 556
            L  LNF  +L ++++++YK
Sbjct: 550 TLSFLNFLVYLWISKRYKYK 563

BLAST of HG10000146 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.4e-115
Identity = 232/560 (41.43%), Postives = 341/560 (60.89%), Query Frame = 0

Query: 15  DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++ 
Sbjct: 11  DGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASK 70

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDD 134
            V+N+ G      L+G F+ADA LGRY T+A+F  I   G+ LLT++ ++PG+ P  C  
Sbjct: 71  SVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CSG 130

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFF 194
            T        A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE++S   FF
Sbjct: 131 ET------CHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 190

Query: 195 NRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGS 254
           N FYF I++G++ A  VLV++Q NVG GWG G+    M IAV+    G+ FYR +KP GS
Sbjct: 191 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 250

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNA--------KVPYTYRFKCLDKAAILDE 314
           PLT + +V++ + +K  +  P   S L + Q+A        K+ +T      DKAA+  E
Sbjct: 251 PLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETE 310

Query: 315 YAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRK 374
                  +   W + TVT++EE+K +++LLPIW+TGI+F ++YSQM T  V Q + + + 
Sbjct: 311 SDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQH 370

Query: 375 VG-GFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVF 434
           +G  F+IP  S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGL+ S+F
Sbjct: 371 MGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIF 430

Query: 435 GMVAAGVVENERKAFAVGQN------TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREA 494
            MV+AG++E  R  +    N        ++ FW +PQ+FLVG  E F ++GQLEFF  +A
Sbjct: 431 SMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 490

Query: 495 PERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----HWLRSNLNKGNLNYFYWLLA 554
           P+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN G+L+YF+WLLA
Sbjct: 491 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 550

Query: 555 VLGLLNFFFFLLLARKHQYK 556
            L  LNF  +L +A+ + YK
Sbjct: 551 GLSFLNFLVYLWIAKWYTYK 563

BLAST of HG10000146 vs. ExPASy TrEMBL
Match: A0A5D3CCF7 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold757G00100 PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 560/591 (94.75%), Postives = 577/591 (97.63%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEYA TDPN  +PWIVSTVTEIEEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATTDPN--NPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R+VG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELK SNDMVALEI+GPISMKA ET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of HG10000146 vs. ExPASy TrEMBL
Match: A0A1S3C263 (protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 560/591 (94.75%), Postives = 577/591 (97.63%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEYA TDPN  +PWIVSTVTEIEEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATTDPN--NPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R+VG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELK SNDMVALEI+GPISMKA ET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEIEGPISMKAAET 589

BLAST of HG10000146 vs. ExPASy TrEMBL
Match: A0A5A7V3P8 (Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2406G00170 PE=3 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 558/591 (94.42%), Postives = 577/591 (97.63%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGG EDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGGEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVG+LHLTSAKSATIVTNFLGALNLLGLLGGFLADA+LGRYLTVATFASITAVGVILLTL
Sbjct: 61  LVGELHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPKEEK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTMVIAVIVLL
Sbjct: 181 TNDPKEEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAI+DEYA TDPN  +PWIVSTVTEIEEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAIIDEYATTDPN--NPWIVSTVTEIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+R+VG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERRVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFFLLLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPISMKA ET
Sbjct: 541 NFFFFLLLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISMKAAET 589

BLAST of HG10000146 vs. ExPASy TrEMBL
Match: A0A0A0LQY5 (Oligopeptide transporter OS=Cucumis sativus OX=3659 GN=Csa_2G416080 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 554/591 (93.74%), Postives = 572/591 (96.79%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPK EK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYA T PN  +PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYATTGPN--NPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+RKVG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFF+LLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPIS KA ET
Sbjct: 541 NFFFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of HG10000146 vs. ExPASy TrEMBL
Match: J7LEY9 (Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.3 PE=2 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 553/591 (93.57%), Postives = 571/591 (96.62%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MVLVSKHGSGGEEDD AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY
Sbjct: 1   MVLVSKHGSGGEEDDTAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVA FASITAVGVILLTL
Sbjct: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASITAVGVILLTL 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           TNDPK EK+MIFFFNRFYFAISIGSLFAV+VLVYVQD VGRGWGYGISGGTM +AVIVLL
Sbjct: 181 TNDPKGEKAMIFFFNRFYFAISIGSLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPS+LNDYQNAKVPYTYRFKCLDK
Sbjct: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDK 300

Query: 301 AAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQA 360
           AAILDEYA T PN  +PWIVSTVTE+EEVKMV KL+PIWSTGILFWTIYSQMTTFTVEQA
Sbjct: 301 AAILDEYATTGPN--NPWIVSTVTEVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQA 360

Query: 361 SFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420
           SFM+RKVG FEIPPGSMSAFLFIAILLVTS+NEKLFIPIARKLTHNVQGLTSLQRIGIGL
Sbjct: 361 SFMERKVGSFEIPPGSMSAFLFIAILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGL 420

Query: 421 LFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAP 480
           +FS+FGMVAAG VE ERKA AVGQ+TRISAFWLIPQFFLVGAGEAF YVGQLEFFIREAP
Sbjct: 421 VFSIFGMVAAGAVEKERKASAVGQSTRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAP 480

Query: 481 ERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVLGLL 540
           E+MKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK WLRSNLNKG+L+YFYWLLAVLGLL
Sbjct: 481 EQMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLL 540

Query: 541 NFFFFLLLARKHQYKDQHYVSSKD-NEELKTSNDMVALEIDGPISMKATET 591
           NFFFF+LLARKHQYKDQHYVSSKD NEELK SNDMVALE++GPIS KA ET
Sbjct: 541 NFFFFILLARKHQYKDQHYVSSKDNNEELKISNDMVALEMEGPISKKAAET 589

BLAST of HG10000146 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 813.1 bits (2099), Expect = 1.4e-235
Identity = 408/558 (73.12%), Postives = 463/558 (82.97%), Query Frame = 0

Query: 1   MVLVSKHGSGGEEDDAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTY 60
           MV VS      +  + A D+ GNP DKSKTGGWL AGLILG+ELSERICVMGISMNLVTY
Sbjct: 1   MVHVSSSHGAKDGSEEAYDYRGNPPDKSKTGGWLGAGLILGSELSERICVMGISMNLVTY 60

Query: 61  LVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTL 120
           LVGDLH++SAKSATIVTNF+G LNLLGLLGGFLADAKLGRY  VA  AS+TA+GV+LLT+
Sbjct: 61  LVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASVTALGVLLLTV 120

Query: 121 ATTIPGMRPPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFD 180
           ATTI  MRPP CDD  R  HQCIEANG QLA+LY ALYTIALGGGGIKSNVSGFGSDQFD
Sbjct: 121 ATTISSMRPPICDDFRRLHHQCIEANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFD 180

Query: 181 TNDPKEEKSMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLL 240
           T+DPKEEK MIFFFNRFYF+IS+GSLFAV+ LVYVQDNVGRGWGYGIS  TMV+A IVLL
Sbjct: 181 TSDPKEEKQMIFFFNRFYFSISVGSLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLL 240

Query: 241 CGTTFYRFKKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNAKVPYTYRFKCLDK 300
           CGT  YRFKKP+GSP T IWRV  LAWKKR   +PAHPS LN Y N  VP+T   KCLDK
Sbjct: 241 CGTKRYRFKKPKGSPFTTIWRVGFLAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDK 300

Query: 301 AAIL---DEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTV 360
           AAI       ++ D    DPWIVSTVT++EEVK+V+KL+PIW+T ILFWTIYSQMTTFTV
Sbjct: 301 AAISKNESSPSSKDFEEKDPWIVSTVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTV 360

Query: 361 EQASFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIG 420
           EQA+FM RK+G F +P GS SAFL + ILL TS+NE++F+P+ R+LT   QG+TSLQRIG
Sbjct: 361 EQATFMDRKLGSFTVPAGSYSAFLILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIG 420

Query: 421 IGLLFSVFGMVAAGVVENERKAFAVGQNTRISAFWLIPQFFLVGAGEAFAYVGQLEFFIR 480
           +GL+FS+  M  A V+EN R+  AV  + +ISAFWL+PQ+FLVGAGEAFAYVGQLEFFIR
Sbjct: 421 VGLVFSMAAMAVAAVIENARREAAVNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIR 480

Query: 481 EAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKHWLRSNLNKGNLNYFYWLLAVL 540
           EAPERMKSMSTGLFLST+SMGFFVSSLLV++VD+VT+K WLRSNLNK  LNYFYWLL VL
Sbjct: 481 EAPERMKSMSTGLFLSTISMGFFVSSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVL 540

Query: 541 GLLNFFFFLLLARKHQYK 556
           G LNF  F++ A KHQYK
Sbjct: 541 GALNFLIFIVFAMKHQYK 558

BLAST of HG10000146 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 543.1 bits (1398), Expect = 2.7e-154
Identity = 287/567 (50.62%), Postives = 386/567 (68.08%), Query Frame = 0

Query: 12  EEDDAAVDFW---GNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLT 71
           + DD  +D W   G P D+SKTGGW +A +IL  E  ER+  +GI +NLVTYL G +HL 
Sbjct: 7   KSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLG 66

Query: 72  SAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMR 131
           +A +A  VTNFLG   +L LLGGF+AD  LGRYLT+A FA+I A GV +LTL+T IPG+R
Sbjct: 67  NATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLR 126

Query: 132 PPHCDDSTRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEK 191
           PP C+ +T     C +A+G QL +LY ALY  ALG GG+K++VSGFGSDQFD  +PKE  
Sbjct: 127 PPRCNPTTSS--HCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERS 186

Query: 192 SMIFFFNRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRF 251
            M +FFNRF+F I++GSL AV VLVYVQD+VGR WGYGI    +V+A+ V L GT  YRF
Sbjct: 187 KMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRF 246

Query: 252 KKPRGSPLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQN-----------AKVPYTYRFKC 311
           KK  GSP+T +  V++ AW+ R L  PA PSYL D  +            K+P+T +F+ 
Sbjct: 247 KKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRS 306

Query: 312 LDKAAILDEYAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTV 371
           LDKAAI D+ A    N ++ W +ST+T++EEVK ++++LPIW+T ILFWT+++Q+TT +V
Sbjct: 307 LDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSV 366

Query: 372 EQASFMQRKVGGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIG 431
            Q+  + R +G FEIPP SM+ F    +LL T++ +++ I + +KL +   GL  LQRIG
Sbjct: 367 AQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIG 426

Query: 432 IGLLFSVFGMVAAGVVENE--RKAFAVGQNTR---ISAFWLIPQFFLVGAGEAFAYVGQL 491
           +GL F    M  A +VE +  R A A G   +   +  + LIPQ+ +VG GEA  Y GQL
Sbjct: 427 LGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQL 486

Query: 492 EFFIREAPERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK--HWLRSNLNKGNLNYF 551
           +FF+RE P+ MK MSTGL LSTL++GFF SS+LVTIV+K T K   W+  +LNKG L  F
Sbjct: 487 DFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNF 546

Query: 552 YWLLAVLGLLNFFFFLLLARKHQYKDQ 558
           YWL+AVL  LNF  FL+ ++ + YK++
Sbjct: 547 YWLVAVLVALNFLIFLVFSKWYVYKEK 571

BLAST of HG10000146 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 1.3e-148
Identity = 281/549 (51.18%), Postives = 381/549 (69.40%), Query Frame = 0

Query: 17  AVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSATIV 76
           AVD+ G P DKSKTGGW+ A LILG E+ ER+  MGI++NLVTYL+  +HL S+ SA IV
Sbjct: 12  AVDYKGRPADKSKTGGWITAALILGIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIV 71

Query: 77  TNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDDST 136
           T+F+G   LL LLGGFLAD+ LGR+ T+  F++I A+G   L +AT +P +RPP C    
Sbjct: 72  TDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTCHHG- 131

Query: 137 RQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFFNR 196
                CI A   Q+ +LY +LY IALG GG+KS++SGFGSDQFD  DPKE+  M FFFNR
Sbjct: 132 ---EACIPATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNR 191

Query: 197 FYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPL 256
           F+F IS+G+L AV VLVY+QD VGR W YGI   +M IA+++ LCGT  YR+KK +GSP+
Sbjct: 192 FFFFISMGTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPV 251

Query: 257 TVIWRVVLLAWKKRSLPHPAHPSYL--NDYQNAKVPYTYRFKCLDKAAILDE-------Y 316
             I++V+  A++KR +  P    YL  ++ +  ++ +T +F  LDKAAI+ E        
Sbjct: 252 VQIFQVIAAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLD 311

Query: 317 AATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRKV 376
               PN   PW +S+VT++EEVKM+++LLPIW+T I+FWT Y+QM TF+VEQAS M+R +
Sbjct: 312 GVAIPN---PWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNI 371

Query: 377 GGFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVFGM 436
           G F+IP GS++ F   AIL+  ++ ++  +P  +K      G +SLQRI IGL+ S  GM
Sbjct: 372 GSFKIPAGSLTVFFVAAILITLAVYDRAIMPFWKK-WKGKPGFSSLQRIAIGLVLSTAGM 431

Query: 437 VAAGVVENERKAFAVGQNTR---ISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMK 496
            AA +VE +R + A   + +   IS F L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK
Sbjct: 432 AAAALVEQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMK 491

Query: 497 SMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKH----WLRSNLNKGNLNYFYWLLAVLGLL 550
           +MSTGLFL+TLS+GFFVSS LV+IV +VT+      WL  N+N G L+YFYWLL +L  +
Sbjct: 492 TMSTGLFLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGI 551

BLAST of HG10000146 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.6e-125
Identity = 241/560 (43.04%), Postives = 353/560 (63.04%), Query Frame = 0

Query: 15  DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  VD   NP +K KTG W A   ILG E  ER+   G+  NLV YL   L+  +A +A 
Sbjct: 10  DGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAAN 69

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDD 134
            VTN+ G   +  L+G F+ADA LGRY T+ATF  I   G+ LLTL+ ++PG++P +C+ 
Sbjct: 70  NVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNA 129

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFF 194
            T         N  Q A+ + ALY IALG GGIK  VS FG+DQFD ND  E+     FF
Sbjct: 130 DT------CHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 189

Query: 195 NRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGS 254
           N FYF+I++G+L A  VLV++Q NVG GWG+G+    MVIAV     G+ FYR ++P GS
Sbjct: 190 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 249

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSYL--------NDYQNAKVPYTYRFKCLDKAAILDE 314
           PLT I++V++ A++K S+  P   S L        N   + K+ +T   K  DKAA+  +
Sbjct: 250 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 309

Query: 315 YAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRK 374
             +      +PW + +VT++EE+K ++ LLP+W+TGI+F T+YSQM+T  V Q + M + 
Sbjct: 310 SDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQH 369

Query: 375 VG-GFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVF 434
           +G  FEIP  S+S F  +++L  T + ++  IP+ARK T N +G T LQR+GIGL+ S+F
Sbjct: 370 MGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIF 429

Query: 435 GMVAAGVVENERKAFAVGQNT------RISAFWLIPQFFLVGAGEAFAYVGQLEFFIREA 494
            M+ AGV+E  R  +    N        +S FW IPQ+ L+G  E F ++GQLEFF  +A
Sbjct: 430 AMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQA 489

Query: 495 PERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNKH----WLRSNLNKGNLNYFYWLLA 554
           P+ M+S+ + L L+T+++G ++S++LVT+V K+T K+    W+  NLN+G+L+YF++LLA
Sbjct: 490 PDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLA 549

Query: 555 VLGLLNFFFFLLLARKHQYK 556
            L  LNF  +L ++++++YK
Sbjct: 550 TLSFLNFLVYLWISKRYKYK 563

BLAST of HG10000146 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 417.5 bits (1072), Expect = 1.7e-116
Identity = 232/560 (41.43%), Postives = 341/560 (60.89%), Query Frame = 0

Query: 15  DAAVDFWGNPVDKSKTGGWLAAGLILGTELSERICVMGISMNLVTYLVGDLHLTSAKSAT 74
           D  +D    P +K+KTG W A   ILGTE  ER+   G+S NL+ YL   +++ +  ++ 
Sbjct: 11  DGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASK 70

Query: 75  IVTNFLGALNLLGLLGGFLADAKLGRYLTVATFASITAVGVILLTLATTIPGMRPPHCDD 134
            V+N+ G      L+G F+ADA LGRY T+A+F  I   G+ LLT++ ++PG+ P  C  
Sbjct: 71  SVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT-CSG 130

Query: 135 STRQLHQCIEANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKSMIFFF 194
            T        A  GQ A+ + ALY IALG GGIK  VS FG+DQFD  D KE++S   FF
Sbjct: 131 ET------CHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 190

Query: 195 NRFYFAISIGSLFAVVVLVYVQDNVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGS 254
           N FYF I++G++ A  VLV++Q NVG GWG G+    M IAV+    G+ FYR +KP GS
Sbjct: 191 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 250

Query: 255 PLTVIWRVVLLAWKKRSLPHPAHPSYLNDYQNA--------KVPYTYRFKCLDKAAILDE 314
           PLT + +V++ + +K  +  P   S L + Q+A        K+ +T      DKAA+  E
Sbjct: 251 PLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETE 310

Query: 315 YAATDPNRYDPWIVSTVTEIEEVKMVLKLLPIWSTGILFWTIYSQMTTFTVEQASFMQRK 374
                  +   W + TVT++EE+K +++LLPIW+TGI+F ++YSQM T  V Q + + + 
Sbjct: 311 SDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQH 370

Query: 375 VG-GFEIPPGSMSAFLFIAILLVTSMNEKLFIPIARKLTHNVQGLTSLQRIGIGLLFSVF 434
           +G  F+IP  S+S F  +++L    + +KL +P ARK T + +G T LQRIGIGL+ S+F
Sbjct: 371 MGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIF 430

Query: 435 GMVAAGVVENERKAFAVGQN------TRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREA 494
            MV+AG++E  R  +    N        ++ FW +PQ+FLVG  E F ++GQLEFF  +A
Sbjct: 431 SMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQA 490

Query: 495 PERMKSMSTGLFLSTLSMGFFVSSLLVTIVDKVTNK----HWLRSNLNKGNLNYFYWLLA 554
           P+ M+S+ + L L+ ++ G ++S+ LVT+V KVT       W+  NLN G+L+YF+WLLA
Sbjct: 491 PDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLA 550

Query: 555 VLGLLNFFFFLLLARKHQYK 556
            L  LNF  +L +A+ + YK
Sbjct: 551 GLSFLNFLVYLWIAKWYTYK 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455932.10.0e+0094.75PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo] >TYK09533.1 protein NRT1/... [more]
KAA0062862.10.0e+0094.42protein NRT1/ PTR FAMILY 6.4 [Cucumis melo var. makuwa][more]
XP_011649985.10.0e+0093.74protein NRT1/ PTR FAMILY 6.4 [Cucumis sativus] >KGN63217.1 hypothetical protein ... [more]
AFR11353.10.0e+0093.57nitrate transporter [Cucumis sativus][more]
XP_038901295.10.0e+0093.40protein NRT1/ PTR FAMILY 6.4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LVE02.0e-23473.12Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1[more]
Q050853.9e-15350.62Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1[more]
Q9SZY41.9e-14751.18Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1[more]
Q9M3906.4e-12443.04Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB82.4e-11541.43Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CCF70.0e+0094.75Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3C2630.0e+0094.75protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo OX=3656 GN=LOC103495995 PE=3 SV=1[more]
A0A5A7V3P80.0e+0094.42Protein NRT1/ PTR FAMILY 6.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A0A0LQY50.0e+0093.74Oligopeptide transporter OS=Cucumis sativus OX=3659 GN=Csa_2G416080 PE=3 SV=1[more]
J7LEY90.0e+0093.57Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G21670.11.4e-23573.12Major facilitator superfamily protein [more]
AT1G12110.12.7e-15450.62nitrate transporter 1.1 [more]
AT2G26690.11.3e-14851.18Major facilitator superfamily protein [more]
AT3G54140.14.6e-12543.04peptide transporter 1 [more]
AT5G01180.11.7e-11641.43peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..561
e-value: 6.6E-182
score: 607.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 27..556
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 101..515
e-value: 1.5E-109
score: 366.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 13..566
NoneNo IPR availablePANTHERPTHR11654:SF373PROTEIN NRT1/ PTR FAMILY 6.4-LIKEcoord: 13..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000146.1HG10000146.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity