HG10000073 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10000073
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Nitrile-specifier protein 5
Location	Chr09: 568707 .. 569784 (+)
RNA-Seq Expression	HG10000073
Synteny	HG10000073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCGACGAATGGAAAATGGATTAAGGTCAGTATCTTCAACTAATCATAATCATCTATTCAATTTTCAATTCCATCTTTTCACAAATTTTCATCTACATTTTCCAAATCTTCAACAAAATTCTTTTCAGATCGATCAAAAGACCACAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGGCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCTCCACGAGTCCCCGTCGACAACCAACTCCACGTCTTCGACCTCGAACAACACATCTGGACGGTCGCCGCCGTCTCCGGCGACGTCCCTCCGCCGCGTGTCGGCGTCACGATGGCCTCCGTCGGCCAAATCGTTTACGTCTTCGGTGGTAGGGATTCAGAGCACAAGGAGCTAAACGAGCTCTACTCCTTCGACACATCCATCAACAAATGGACGCTCCTCTCCGGCGGCGACTCCGGACCACCGAATCGGAGCTACCACTCCACCACGGCCGATGATCGACGCGTGTACATCTTCGGCGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACTGTAGATGGAAAATGGATCGAGTATCCAGCAGGAGGGGAAGGGTTAAAGGGCCGCGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTTGATCCGGCCCAAAAAAAATGGGCCCAAGTTGAAACAAGCGGAGAAAAGCCGACGGCTCGGAGCGTGTTCTGCACGTGCGCGATTGGGAAATACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGCTTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCTTGGAGAATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTGCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTGTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGA

mRNA sequence

ATGGCTCCGACGAATGGAAAATGGATTAAGATCGATCAAAAGACCACAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGGCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCTCCACGAGTCCCCGTCGACAACCAACTCCACGTCTTCGACCTCGAACAACACATCTGGACGGTCGCCGCCGTCTCCGGCGACGTCCCTCCGCCGCGTGTCGGCGTCACGATGGCCTCCGTCGGCCAAATCGTTTACGTCTTCGGTGGTAGGGATTCAGAGCACAAGGAGCTAAACGAGCTCTACTCCTTCGACACATCCATCAACAAATGGACGCTCCTCTCCGGCGGCGACTCCGGACCACCGAATCGGAGCTACCACTCCACCACGGCCGATGATCGACGCGTGTACATCTTCGGCGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACTGTAGATGGAAAATGGATCGAGTATCCAGCAGGAGGGGAAGGGTTAAAGGGCCGCGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTTGATCCGGCCCAAAAAAAATGGGCCCAAGTTGAAACAAGCGGAGAAAAGCCGACGGCTCGGAGCGTGTTCTGCACGTGCGCGATTGGGAAATACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGCTTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCTTGGAGAATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTGCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTGTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGA

Coding sequence (CDS)

ATGGCTCCGACGAATGGAAAATGGATTAAGATCGATCAAAAGACCACAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGGCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCTCCACGAGTCCCCGTCGACAACCAACTCCACGTCTTCGACCTCGAACAACACATCTGGACGGTCGCCGCCGTCTCCGGCGACGTCCCTCCGCCGCGTGTCGGCGTCACGATGGCCTCCGTCGGCCAAATCGTTTACGTCTTCGGTGGTAGGGATTCAGAGCACAAGGAGCTAAACGAGCTCTACTCCTTCGACACATCCATCAACAAATGGACGCTCCTCTCCGGCGGCGACTCCGGACCACCGAATCGGAGCTACCACTCCACCACGGCCGATGATCGACGCGTGTACATCTTCGGCGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACTGTAGATGGAAAATGGATCGAGTATCCAGCAGGAGGGGAAGGGTTAAAGGGCCGCGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTTGATCCGGCCCAAAAAAAATGGGCCCAAGTTGAAACAAGCGGAGAAAAGCCGACGGCTCGGAGCGTGTTCTGCACGTGCGCGATTGGGAAATACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGCTTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCTTGGAGAATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTGCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTGTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGA

Protein sequence

MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLVVYGGNSPSNDRLGDIYFFAPCE

Homology

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BLAST of HG10000073 vs. NCBI nr
Match: XP_038900567.1 (nitrile-specifier protein 5 [Benincasa hispida])

HSP 1 Score: 651.7 bits (1680), Expect = 3.2e-183
Identity = 299/324 (92.28%), Postives = 310/324 (95.68%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPTNGKW KIDQKT GPGARSSHAITIV QKAYAFGGEFSPRVPVDN+LHVFDL +HIW
Sbjct: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA VSGDVPPPRVGVTMASVG+I+YVFGGRDSEH ELNELYSFDTS +KWTLLSG DSG
Sbjct: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKW+EYPAGGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFD AQ +WAQVETSGEKPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSDLGHLGAGKFAGE YVLDTEALEWSRVEDGLG+ENHPGPRGWCAF+VGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of HG10000073 vs. NCBI nr
Match: XP_004139996.1 (nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_004139998.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_011656888.1 nitrile-specifier protein 5 [Cucumis sativus] >KGN46612.1 hypothetical protein Csa_004873 [Cucumis sativus])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 293/324 (90.43%), Postives = 306/324 (94.44%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPT G WIKIDQKTTGPGARSSHAI IV QKAY+FGGEFSPR+PVDN+LH+FDL  HIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TV A SGD+PPPRVGVTMASVGQI+YVFGGRD+EHKELNELYSFDTSINKWTLLSGGD G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDT+DGKWIEYPA GEGLKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF P Q KW QVET GE+PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSDLGHLGAGKF GE YVLDTEALEWSRVEDGLGLE+HPGPRGWCAFAVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

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BLAST of HG10000073 vs. NCBI nr
Match: XP_008464157.1 (PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >KAA0060815.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa] >TYK08820.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 647.5 bits (1669), Expect = 6.1e-182
Identity = 292/324 (90.12%), Postives = 309/324 (95.37%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPTNGKWIKIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDN+LHVFDL  HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TV AVSGD+PPPRVGVTMAS+GQI+YVFGGRD+EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKWIEYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDP Q KWAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSD+GHLGAGKFAGE YVLDTE LEWSRV+DGLG+E+HPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of HG10000073 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 641.0 bits (1652), Expect = 5.7e-180
Identity = 292/322 (90.68%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAP NGKW KIDQ   GPGARSSHAITIVG+KAYAFGGEFSPR+PVDN+LHVFDL++H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA+VSGDVPPPRVGVTM +VGQIVYVFGGRD EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT+DGKWIEYP+ GEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQ KWAQVET+GE+PT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSDLGHLGAGKFAGE YVLDTEALEWSRVEDGLGLENHPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAP 323
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of HG10000073 vs. NCBI nr
Match: XP_022947523.1 (nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 290/322 (90.06%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAP NGKW KIDQ   GPGARSSHAIT+VG+KAYAFGGEFSPR+PVDN+LHVFDL++H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA+VSGDVPPPRVGVTMA+VGQIVYVFGGRD EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT++GKWIEYP+ GEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQ KWAQVET+GE+PTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSD+GHLGAGKFAGE YVLDTEALEWSRVEDGLGLENHPGPRGW AF+VGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAP 323
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of HG10000073 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 6.2e-105
Identity = 171/320 (53.44%), Postives = 221/320 (69.06%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           M P   KW+K+ QK  GPGARSSHA+T+VG K Y FGGE  P + +DN L+VFDLE   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           ++A  +G+ P P  GV+M ++G  +YV+GGRD + +  N L+S+DT  N+W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD VD KW+EYPA GE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD A ++W  VET+G+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           E +P +L H+GAGK +GE Y LDTE L W R+  G   E  P  RGWCAF    K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LVVYGGNSPSNDRLGDIYFF 321
           L+V+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of HG10000073 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 347.4 bits (890), Expect = 1.7e-94
Identity = 158/316 (50.00%), Postives = 208/316 (65.82%), Query Frame = 0


Query: 6   GKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAV 65
           GKWIK++QK  GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDLE   W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNR 125
           +GDVP    +GV M SVG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q KW QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLVVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A       G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of HG10000073 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.5e-94
Identity = 155/324 (47.84%), Postives = 212/324 (65.43%), Query Frame = 0


Query: 1   MAPT---NGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQ 60
           ++PT   +GKW K+D+   GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD+E 
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVAAVSGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSG 120
             W+++  +GD+P    +GV M S+G  +YVFGGRD+  ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GDSGPPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGG 180
            + GP  RS+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+DP Q KW QVET G +P+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKG 300
           I+GGE+    L H+G G+     + LDTE L+W R++   G E  P  RGW A      G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLVVYGGNSPSNDRLGDIYFF 321
           G+ GLV++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

[Close]

BLAST of HG10000073 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.1e-91
Identity = 158/317 (49.84%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 6   GKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAV 65
           GKWIK++QK  GPG R SH I  VG K ++FGGE +P  P+D  L+VFDLE   W+++  
Sbjct: 152 GKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSISPA 211

Query: 66  SGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNR 125
           +GDVP    +GV M S+G  +YVFGGRD+  K  N  YSFDT+ N+W LL+  + GP  R
Sbjct: 212 TGDVPNLSCLGVRMVSIGSSLYVFGGRDASRK-YNGFYSFDTTKNEWKLLTPVEQGPTPR 271

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQD 185
           S+HS TAD+  VY+FGG   + RL  L AY+ VD KW++    G     RGG GL V Q 
Sbjct: 272 SFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVVQG 331

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVH +DPAQ KW QVET GEKP ARSVF +  +GK++ ++GGE+  
Sbjct: 332 KVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEIAM 391

Query: 246 SDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENH-PGPRGWCAFAVGRKGGELGLVV 305
               H G G+ +G  + LDTE L+W ++ D LG E   P  RGW A   G   G+ GLV+
Sbjct: 392 DPKAHEGPGQLSGGTFALDTETLKWEKL-DKLGEEEETPSIRGWSASTTGTIDGKKGLVM 451

Query: 306 YGGNSPSNDRLGDIYFF 321
           +GG + +NDR GD++F+
Sbjct: 452 FGGKAQTNDRFGDLFFY 466

[Close]

BLAST of HG10000073 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.3e-88
Identity = 150/316 (47.47%), Postives = 198/316 (62.66%), Query Frame = 0


Query: 6   GKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAV 65
           G WIK++Q   GPG R SH I  VG K Y+FGGE  P  P+D  L+VFDLE   W++A  
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNR 125
           +GDVP    +GV M SVG  +Y FGGRD   ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG G   R+  L +Y+ VD  W      G+    RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+W+VYGF G E+DDVH++DPA+ KW QVET G KP  RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLVVY 305
               H+G G+     + LDTE L+W R++     E  P  RGW A   G   G+ GLV++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

[Close]

BLAST of HG10000073 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 5.9e-183
Identity = 293/324 (90.43%), Postives = 306/324 (94.44%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPT G WIKIDQKTTGPGARSSHAI IV QKAY+FGGEFSPR+PVDN+LH+FDL  HIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TV A SGD+PPPRVGVTMASVGQI+YVFGGRD+EHKELNELYSFDTSINKWTLLSGGD G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDT+DGKWIEYPA GEGLKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF P Q KW QVET GE+PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSDLGHLGAGKF GE YVLDTEALEWSRVEDGLGLE+HPGPRGWCAFAVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

[Close]

BLAST of HG10000073 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 2.9e-182
Identity = 292/324 (90.12%), Postives = 309/324 (95.37%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPTNGKWIKIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDN+LHVFDL  HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TV AVSGD+PPPRVGVTMAS+GQI+YVFGGRD+EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKWIEYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDP Q KWAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSD+GHLGAGKFAGE YVLDTE LEWSRV+DGLG+E+HPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of HG10000073 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 2.9e-182
Identity = 292/324 (90.12%), Postives = 309/324 (95.37%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAPTNGKWIKIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDN+LHVFDL  HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TV AVSGD+PPPRVGVTMAS+GQI+YVFGGRD+EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKWIEYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDP Q KWAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSD+GHLGAGKFAGE YVLDTE LEWSRV+DGLG+E+HPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAPCE 325
           L+VYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of HG10000073 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.8e-180
Identity = 292/322 (90.68%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAP NGKW KIDQ   GPGARSSHAITIVG+KAYAFGGEFSPR+PVDN+LHVFDL++H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA+VSGDVPPPRVGVTM +VGQIVYVFGGRD EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT+DGKWIEYP+ GEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQ KWAQVET+GE+PT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSDLGHLGAGKFAGE YVLDTEALEWSRVEDGLGLENHPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAP 323
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of HG10000073 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.0e-180
Identity = 290/322 (90.06%), Postives = 308/322 (95.65%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           MAP NGKW KIDQ   GPGARSSHAIT+VG+KAYAFGGEFSPR+PVDN+LHVFDL++H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           TVA+VSGDVPPPRVGVTMA+VGQIVYVFGGRD EHKELNELYSFDTSINKWTLLSGG+SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
           PP+RSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT++GKWIEYP+ GEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQ KWAQVET+GE+PTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           EVDPSD+GHLGAGKFAGE YVLDTEALEWSRVEDGLGLENHPGPRGW AF+VGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LVVYGGNSPSNDRLGDIYFFAP 323
           L+VYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of HG10000073 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 490.7 bits (1262), Expect = 8.8e-139
Identity = 218/320 (68.12%), Postives = 254/320 (79.38%), Query Frame = 0


Query: 3   PTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTV 62
           P  GKW+++ QK TGPGARSSHAI +VG K YAFGGEF PRVPVDNQL+VFDLE   W++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  AAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPP 122
              SGD PPPRVGV MA+VG I+Y FGGRDS H+ELNELY F+T  N+W LLS G++GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 NRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVA 182
           NRSYHS TAD + VY+FGGCG  GRLNDLWAY+ VD KWI++P+ GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEV 242
           Q KIWVVYGFAG E DDVH FD A+ +W +VET GEKP+ARSVF T  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLV 302
           DPSDLGH+GAG F G+AY LDTE LEW + EDG+G   HPGPRGWCAFA G + G+ GL+
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFAP 323
           VYGGNSPSNDRL DI+FF P
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

[Close]

BLAST of HG10000073 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 382.1 bits (980), Expect = 4.4e-106
Identity = 171/320 (53.44%), Postives = 221/320 (69.06%), Query Frame = 0


Query: 1   MAPTNGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIW 60
           M P   KW+K+ QK  GPGARSSHA+T+VG K Y FGGE  P + +DN L+VFDLE   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVAAVSGDVPPPRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSG 120
           ++A  +G+ P P  GV+M ++G  +YV+GGRD + +  N L+S+DT  N+W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD VD KW+EYPA GE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD A ++W  VET+G+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELG 300
           E +P +L H+GAGK +GE Y LDTE L W R+  G   E  P  RGWCAF    K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LVVYGGNSPSNDRLGDIYFF 321
           L+V+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of HG10000073 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 347.4 bits (890), Expect = 1.2e-95
Identity = 158/316 (50.00%), Postives = 208/316 (65.82%), Query Frame = 0


Query: 6   GKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAV 65
           GKWIK++QK  GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDLE   W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNR 125
           +GDVP    +GV M SVG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q KW QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLVVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A       G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of HG10000073 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 347.4 bits (890), Expect = 1.2e-95
Identity = 158/316 (50.00%), Postives = 208/316 (65.82%), Query Frame = 0


Query: 6   GKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQHIWTVAAV 65
           GKWIK++QK  GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDLE   W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSGGDSGPPNR 125
           +GDVP    +GV M SVG  +YVFGGRD+  ++ N  YSFDT+ N+W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q KW QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKGGELGLVVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A       G+ GLV++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of HG10000073 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 345.1 bits (884), Expect = 6.0e-95
Identity = 155/324 (47.84%), Postives = 212/324 (65.43%), Query Frame = 0


Query: 1   MAPT---NGKWIKIDQKTTGPGARSSHAITIVGQKAYAFGGEFSPRVPVDNQLHVFDLEQ 60
           ++PT   +GKW K+D+   GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD+E 
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVAAVSGDVPP-PRVGVTMASVGQIVYVFGGRDSEHKELNELYSFDTSINKWTLLSG 120
             W+++  +GD+P    +GV M S+G  +YVFGGRD+  ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GDSGPPNRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAGGEGLKGRGG 180
            + GP  RS+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDPAQKKWAQVETSGEKPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+DP Q KW QVET G +P+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEAYVLDTEALEWSRVEDGLGLENHPGPRGWCAFAVGRKG 300
           I+GGE+    L H+G G+     + LDTE L+W R++   G E  P  RGW A      G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLVVYGGNSPSNDRLGDIYFF 321
           G+ GLV++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

The following BLAST results are available for this feature:

BLAST of HG10000073 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900567.1	3.2e-183	92.28	nitrile-specifier protein 5 [Benincasa hispida]	[more]
XP_004139996.1	1.2e-182	90.43	nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier ...	[more]
XP_008464157.1	6.1e-182	90.12	PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED:...	[more]
XP_022971053.1	5.7e-180	90.68	nitrile-specifier protein 5 [Cucurbita maxima]	[more]
XP_022947523.1	1.7e-179	90.06	nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier...	[more]

BLAST of HG10000073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93XW5	6.2e-105	53.44	Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1	[more]
Q9SDM9	1.7e-94	50.00	Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2	[more]
O04316	8.5e-94	47.84	Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1	[more]
O49326	5.1e-91	49.84	Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1	[more]
O04318	5.3e-88	47.47	Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1	[more]

BLAST of HG10000073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KDY9	5.9e-183	90.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1	[more]
A0A1S3CLA8	2.9e-182	90.12	nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1	[more]
A0A5D3CBW1	2.9e-182	90.12	Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A6J1I5Q1	2.8e-180	90.68	nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=...	[more]
A0A6J1G6U4	8.0e-180	90.06	nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 S...	[more]

BLAST of HG10000073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07720.1	8.8e-139	68.13	Galactose oxidase/kelch repeat superfamily protein	[more]
AT5G48180.1	4.4e-106	53.44	nitrile specifier protein 5	[more]
AT3G16400.1	1.2e-95	50.00	nitrile specifier protein 1	[more]
AT3G16400.2	1.2e-95	50.00	nitrile specifier protein 1	[more]
AT3G16410.1	6.0e-95	47.84	nitrile specifier protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 13..171 e-value: 3.9E-34 score: 120.5
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 172..323 e-value: 1.0E-20 score: 75.8
IPR015915	Kelch-type beta propeller	SUPERFAMILY	117281	Kelch motif	coord: 3..288
None	No IPR available	PFAM	PF13418	Kelch_4	coord: 222..274 e-value: 6.3E-5 score: 22.9 coord: 124..161 e-value: 5.6E-12 score: 45.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR47435	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 5..322
None	No IPR available	PANTHER	PTHR47435:SF4	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 5..322
IPR006652	Kelch repeat type 1	PFAM	PF01344	Kelch_1	coord: 174..213 e-value: 4.1E-5 score: 23.1 coord: 72..114 e-value: 4.3E-9 score: 35.8 coord: 20..64 e-value: 1.4E-4 score: 21.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10000073.1	HG10000073.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0080028	nitrile biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0010043	response to zinc ion
cellular_component	GO:0005634	nucleus
molecular_function	GO:0030234	enzyme regulator activity
molecular_function	GO:0005515	protein binding