Cucsat.G9991 (gene) Cucumber (B10) v3

Overview
NameCucsat.G9991
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmannosyl-oligosaccharide glucosidase GCS1-like
Locationctg1673: 2926438 .. 2938246 (+)
RNA-Seq ExpressionCucsat.G9991
SyntenyCucsat.G9991
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACCAAAAGTCAATCAAACAAAGCTAAGAAGAAAATGCCCTTTCTAAATAATGCACTTATTTTTCCTTTTTCTTTCTTCTTTTGTTTCCTTTAAAAAGCTTATTTTCCAATTGAGTATTTAGCGCGAAAAAAGAACCTATTTATATCACCCAGTGAAGCCTCAAGTCAAGGAATTGCCGGAGATAACTCAGAATTAGGAATCTCCAACTCAAACACAACACTTCTCTCTATCACTCCACTCCTTCTTGCGATCGGTAATCACACTTCCTCTACTGCTTTGCACACTTTGATTTCGATATGGCTGGACAAAGTCGACGGAATCCTCGAAGCCGAATTAAATCAGCTTCCGCCGTCGACGACGATGACGACAGCGCCGTCCGTCAAGCTTCTGATAATTATAGACGCCTCCGTGATAGGAATAAATCGAGTTCTTCGATTCGCATCTTCAATGTTAATCTCAAGGTCATGGTAGGTATTTGCTTCGTCGCATTCTTCGTCATCTTGTATCTGATCAGTAGCCTGATCGTAACCGGAGATCATCAGACTCCTAGGGTTATTACGCCATTTCCTGCTCCAAAAGTTACCGACCTTCCTCAGGTAGACTGTTGATTTAACTATTTGTTCATTTTTCTTCTCCATCATTTCTTTTTCGCTGTGTATGTAAACATATTTTTGTTCTCTTATGTCATCCTACATTCGTTGATTTTGGAGACAGTTTCAAGGTGAACATAAAGAGAGCTTGTATTGGGGAACTTATCGACCTCATGTCTATCTTGGAATACGAGCTAGGTACTTCATTGAGTTGAAAATAAAGTTTCGTCATATATTTGTACGATTTATGATTTTTTTCAACAATTGAGTTTACTTATGTTTTCGAAGTTCTAGGATGCTTAAATATTTAATGAGTACGTAGGAAATAGTTTGCTAACTACCACATATTCTTATGGTCCATTTACAAAATGTGTATTACTAGGAATACAATCTCTGTACTGAAAACCGGGAGTGGCTCGATTCTTTGAGTTGTGTTCTGTAAGTCGGTTTATGCCTCCAAGCCATTGTAAATAGTTGCCGTATATTTTTGGATAATAGTTGATGAACGAATTTCTTTGATTCAAACCTCGCAAGGTAGTTATGATCTTTCTGTGGCTAGTTTTTAATACTGAACATAAATTCTTTTGAGTATTTGGAGTTCAATCACTGTGATTATCAGTATATAACTTTGGTCACTGTAATAATAGTCTTGACTTCAGCGAATTCTTCTACATTTATTTTGTTCTTTCCTCATTCTATCAAATGTTTTATGCGAATACCTAGTTCTTTAGCTAATGAGTATATCTTGTATTTATCAGGACTCCACGTTCATTGCTTGCTGGCTTGATGTGGATAGGTGTTCAGAATGGAAGATATGTCATGAGGCATGTGTGCCAAAGTTCCGATGAACTAAGCACATATGGTTGGACTCGTCACAATGCACGTGATTTTGGACATCAAGTTCTTGTTGACCAGGATATGACTCTGGGAACAAGCTTTCTGAAATCGAAGGAGAGTGGTAGTGGCTATGGTGGAGATTGGGCTGTTCGCATTCAAGTTAATAGCAAGAAGTAAATGGCGTTAATACAATAATATTTTAAGCATTCTTTTCATAAACGTGGCTTTAATATTGAAGGGCTGAATGGTTATGTCATCTAACAGATTCTTTTGATGTGTAATCTGTCTTATTATTGAGTCACCTGTACTGGAAGGTTTCATGCGTTTTTCAGAATTTGACTATTAGTTTAATGAGTCGTGAACTATGTGAATATTTTTTCAATCTCATTTAACTTCTTATTTACACGAGACTTGGGCTTAAGGCCTATAATAGATTTTCCCCTTTTTTATCATGTTTCTTCTTTGAGGATAATTGATGCACACTCTTCTTATTTCTTGTTGAATTTGCAGATCTGAGGAGACTGAGGAATTGTTGAAAACTGGGCATCTTTTTTTCTACTTGGCAGATGAAGATGGAAATGCTTTAAGTTTAAGTAGAGATGCCATGAACATTCATGAGACTTCACTCCTAGCATCAGGTTCACGAAGTGATGTAGGAAATTGGCAGTTACATTTAGAATCTAAGGTAAATTCCTGAAAGCATTAGTGTGATCCCTCTCCAATTTTTTCGCTGAAATGTCCTATCTAGAAAAAGTTACTTCTCTCAGTTTCTCTCTTTGGATTCTTCTAACCCGAGTAATTAAAGTTTTCTATATTTGATTTATATGCATTCTTATAGTGGCTAGTGTTGGCATTTACTACTTCAAATGCACTTGCAAGTAGTAATTTCAGCCTATCCTGATTCTCTTGAGCTCCATATTGTTTTAATCATCGCCTATCTTTTTTCTGCATTTCTACACTTTATCTTTTAACGAGCTACAGAATGACAAATCTCCCTGATCATTTGTTCTGCTGTAGTCTCTTCCTCCAACCCCTCTCATCTCAATCAAACATTTTAATTGACTTCTCTTTTTCATTATTGCTTTTTGAAATTAAACTCTTCAATTAAAATCTTAGAGTTTGTGTTGTTATTGGAATAGGAATTGCCTCTGACATACTCTGCTTTCTTTGATCAGGCGCTTGTATTTTTTTTACTTAATCATTGTTATATATCTGGTGACTTCATTTTTCTTTTCATATGGCCCCCTGCAGGATGATCTCGAAGTCCATTTTAGTGGGTTTAAGACGGAGCATTATCACAATTTATCTGAACTTGTTCAGGAAAATATTGGTGGACAAGTAAGGTTAACTTTTCTTCTTATTGTTATTTTTAAATGCATTTGGATGCCTCGTGATGTCTGATTCCCCCTAGATAAGTTTTTGGACTTTATATCTGGGTTGTGTTGGACACTGAACCAACATGTCTTTGTATTATCTTTTTTACCAGGCAAGAAAATTTGGTCGATTGCAGCTCTCTGACACATCTGATGACTCCTCAAATATTTTAGTTTTCCAGGTATGCTTTAATGCAGATGAAATTTTCTATAGCATGGAGAAAGTTGGAATATCCAAAATATATGGGTGGCATCGTTTTCTTGTTTTCATCGGTTTGATTTATTTATTTATTTTATTTCACAGATCTCTGGCAGAATTCCATTTAGAACAGATTTGGCTTTCATTTCTGGCTCTGGTTTACCAAGTTCTAGAGAAGAGGAACGTGTCAACAACCTCACAGGTGTTTACTTTGAATCCTTATTTGGACCCACAAGATCCAGTTAGTAGTTGGCAATTGGCATGACCCATATTTAGAGTTAGGAAAAGAAGTAAATCTTTTTGGTACACTTCTACCACAAAAAAATACTTTTTTTTCCCATTTTCTATAAGGTTTTGTGTGCCTTTATGTTTATAAGATACAACTACATATCATTAAAAGTTGTGAAATTATAAGTGAGGCTACTGAATCCTTTGCTTCAACAATGAACCAAAGAAGAACAGAAAAAAGCAATTGAACTGATTTGAAGTTTTTGTCTGATCTCTTAAAAAATCCATTTTATGTATGTGTGGAGGGTCTAAGTCGCATTTCTTATTTCGTAAGTTTACATTGTTTGGACTTGTCGATTATCCACAAGAAGGGTTATTATTTTTCTTGGCTTCCATGCTTCACGTATTATTTTTGTTCCCACTTCAATTAGGTTCATCATTAACTGATCGATTAAAGGAGAAAGAATTAGAATTTGATACCAGATTTGAGGAGTGCTTTGGTTTGGCAAACAAGGTATTTATAGTTTGGACATATATCTCTTCATCATTTTATCTTCTATATTTTGCACAATAATAATAATCACATCTTAGATACGTGCTGGGACTGGATTAACAGATGGATGCATGATTTTTTTAATCATCAATGTAAACTTTTGTAAAATAATATTTGGTTGAAATGGGTAACGAGAACTGTTGTATAGAATATGGCACATAGTTGGATACTATATTGTTATATGATATATAATTAAATTCACCTTCACCCATAAGCTTAAGCTTAAATCACCCATCAATCCGTGATTTAAGATGGTATTAGAGCAGGTGGTTCAGGGTGGTTATGTGGTCATGTGTTCAAGCCCCTGCATTGTTGTTTCCTTCCCAATTAATATTCATTGTGCTTATTGGGTCTTCCTTCATATATTGAGCTCGCAAGTGAGAGGAAGTGTTAAATGATAAATAATTAAATTTGCTTTCACCCATCAGCTTAAGTTTTCGGGTCAATCTGTGATTCAAGATATATTAAGGTATCAAAAGTTGGATATGCTTACTCCATATGGAAAAATGCATGTTTGGTAACGCTTTTCATGGTTTGAGTTCAATTAATATGTAATGAGTTCCTCTAAAAACTGGCAAAAGCAAAAACCGATTTCCATCTTCCAAATGATATTTTCTTCTTTGTTTCCAGTTTATATACCTATATTCTGTTGAAGTCCTTAATCTTGTAATTATTCTTGGATGGATCTAATTTTGTAATTATGATCAGTTTGATTTAGAATCCACAACTGTTGGTAAGGCTGCTGTTTCAAACTTATTAGGTGGCATTGGTTATTTCTATGGCCAGTCAAAAATTGCACTCCCAGGTGCCTCCCATGTAAGTTGTTGAGCCTTTTCTCATTTCTTGTTAGCATGTCTTGACGATTTTTTTGTAGATCTCATATGATTGTTACAAAACCCCAACCCACAATTGTAGATGTTTTTTCTTTTTTTTTTTAATTAGTCTACTAATTTTTCAATTATATTATCATTAAATTATCTCCATGTAGTGAAATATGCATACCTCTCATCGCAATAGCAAACTTTGCCTTATTTAGAAGATGCTTCATCAATATCCATATTTAATTCGAGAATTCTTATGGAATCTCCTAAGATATTGAGGTTAAGGATTGGATTTTAGCGATTGGGAAATGCTTGACATGAATACTGGAAGATACAAAAGAACGGATGATGTTTGTTCAAATCAGATGCTTGAAAAGGAGATCTCCAATAGAATTGAAGGATTGAATTTTTGTGGGAAGTGCTAGACATACGTACTGGATGGTACAAAAAAAAAACAGATGATGTTTGTTTGGTTGCTTCAAAGTTTTACATGATGTGTGTGCTCAGATTAGGAGATAGAATGTTCCTATAAAATATGACAAAAACAAAAGAAAACAGGGGCAATGTTTCCTCACAAGAAATTCAAAACAATGCACATCAAATTAGAGACTACTTTATTCACTATTCATTTGACTCATTACTCAAATTTTTCATTTAAAATTTCCATTTTTACTAGCTATAAAATCTTTCATTTTCTTATTAGGCCAAGAAATTAACATAATTTACAAACGACCTTTTACTTAAACAGAATCTGTTCTATAGGTTGTACCTCTGTGTCCTTGAGTTCTAAAACATATTTGCTTCAAGATTATTAATCTTTTTGTTTTGTAATTTTTGTTTTTTTCTTTTTCTATTTTTCAGTCTTCATGCATATTTATTCTGTTCCTTTTTTGAAACCTGTGAATACAACACCACATTTATTGCAGCTCGGAAGTCATAATGGTTTCCTTTTTTACTGGCCTGCTGAGCTTTACACTGCTGTTCCATGCCGTCCTGTCTTCCCTAGGGGATTCTTGTGGGATGAAGGCTTTCATCAGCTGCTTATCTGGTAAGTGCTTATTGTTTCTAGACGTGCAGCTCACTTTTATCTTTTCAACAAAAGATAGATTATTTGTCTTTTGGATAAGGCAACAAAGATTCACAATTTTTCATTCTTGACCGGACCTTCTTATTCAATATGGAACAGGCGATGGGATATTCACATCAGCTTAGATATTCTTGGTCACTGGTTGGATTTAATGAACATCGATGGATGGATTCCACGTGAACAAATATTAGGGGCTGAAGCTCTCAGGTGATAGCATGTTTAAATTTGTGGTTTTAAATTACTGTTGATCTCTAATCAGTACCATTCCCTTTCTTTTCTCAATGCAGCAAGGTCCCAGAAGAATTTATTCCTCAGTATCCAAGCAATGCTAACCCGCCAGCATTCTTTCTAGTCTTGCGAGGTAATTATATCTCAATTCCCTGTCATAGTGTTTGTGTAAAGTTATCTTCTCTCTGTCTCCGTTTCGCTCCTTCCTCCACCATTTTATTGTCCCATCTATAGATATTAAAAGAAGACTTTGGTGTAAGCCTCTATGGAATCAGTGAGATGGACCATGGTTATAATGCTAGGAGTACAACGTTAACAACCATGGCCAGTCTTACAAAGCACATTCTTAGTTTATTGTGAGTTTGGCTTTCAATAAAAAATTTATCAATGTCATTGGGAAAAACCCTGGGTCTGCTTATTATTTATTCTCAAACACTGAAGTTATTGGATTGCTTTGAAGCCCGTATTGAAGTTAAAAGAAATTTATGTGATTTTCCCAATTGAAATTGAAGTTAAAGATCCTACTAATGGTAAATTCCACCTTAGATATGGTGATATTCCTCCAAATATCCTTTAAGGCAAATTATTTTTCAAAGATTTTTTGAACTCTTTGGATTGCAAGGTTGATCAAAATTACGCACAATGAGGATATGCTTCAGGTATCTGATTTTGTTGAAGAGGGCATTCCCATATGGTCGGACAAGGAAATCATGTTTGTTTCAGCTCTGAATGAGGCATATCAGAGTCTCTGTGGGAGACTTAATTGTCCTTGGGAAATTTTAGTTAGCATTCCATAGTTTATTGATTTAAAGGGCGGCAAAGGGGATGTGAACATTGAAAGATCTTCCTTTCCTTATTATCCTCTTTCTTACAGCTGAAAAAAAAGAAAAAGCAACCATCTGCGAAGGAACATTCAAAGTTCAATTCAGATGGTTTGGAGAAATCCTGTACATTTCTCCTATTGGCAGATGCACATCCCATAGACAATCCACATCCTTTGCCTTCTGATTGAATTTTAGGCAATTAATCACTAAAATCCTGTGATCAACTATTTTTTTCATTAAAGAAGTTTATAGTCATCCATCTCCTTAGGACAATCTTTCTGGTTACCAGTTTGATGGAGATTTAGCTATTAGTGAAAAGTAGTGAGGATATTATGACATTCTAGCATAAGATGATTTTGTTGTGGAAAATTCAGCTGTTTACTTCAAGGTTTTGATATTATGAAAATGTTCAAGCAAAATTGACTAAAAAATGTGGTTCGGCAATGATTTCAATTCCACTTCGTTTTCCTTCCGTTAAAGTTTAGCTTGGTGGTTTAGTCCAGAAATTGAACTATTATAGTGGAATTCTTGTGTTGGTACGACTAAGCCTTACTGTATTGTTCTTAAGAAGTTGCATCTTTTCCTAGTAGTTGCAAGCTTGAAAGCAACAGTTTAGAAGTTGGTTGACTTTGTGGTTTGACACTCGAACTCTTGGTCTTGATGGACTTCTTGCCTCGTTTGCTTGTTGGTCTTCTGTTGGCTTTGGCTTAACGCTCATGTGTTCTCTAGCTTTGGTTTCCTTTCTGGATCTGGTTTGTTATGTTTTAAGTGTTTGCCTTTTGAGATGTCTTATCCTCTTTGTATCTCAGTAGTATTGTCTTTTTTTGTTTTTTTTCATCTTTATTCTCTTGAAGGCTAATGTATTTTTAGGCATTGGTCTCTTTTCATTTTTCCAATGGAAAGTTTTGTTTCCCTAAAAATGTTCTTACTGTGGGGCAATAATTACAGAAAAATTGTGATGGTTATGGTGTTGACTGTTGAGACATAATAGCTTAAGTTTTGAACTCCAATAATTTTTTAAAATGTTTTTTGATGGAGGAATCCTTTTTAACAACGCTGAGGTTTCCTAATAAGTCTGCTACGCGAAAGATACGTTGTAGTAAGCACAAAAGTTTGATATTCTTCTTTCTTTATTATCTGAAAAAGAGACAAAACACGTTGATGACCATTGATCAAAGGATCCATACAACTTCTATGGATCCTTTGATAACGAGTACCTTGCTAGTTATTCATGCTATTTTACATTTTTATTTGATGAGAACTTATTTCTTGGAGTCTTATCTAGTCAGCATTTGGTTACATTCCCATTTACATTTGAGTGCATTGAGAGATCTGATCATGCATGGGTGCTGTGGTTGTTTAACAAGCTCTACATTTTCTCTTTTCTATGCAATATCAACTGTGAATATGAGCTCACAGTACACTGGTAATAGTAGATTCTTGTGGTCCATAACATACACTATCAAATAATTTGGTTGAATCTTTAGTTGTGATGTTCGTGATTAGATGTGTTTTGTCTTGTAGAATTGATTCATGGCCTAAAGAAAAATGCATTTACCGAGACAGAAAGCTCAGAGATATCTTCATTCTTCGAACGGTCTTTTGTCCGCTTGGAAGCATGGTTCCAGTGGTTTAATACTACACAGCCAGGTGTCTGCATCCCGTGCTTTATTATTAAGGGCATTTAATGGTTCTTTCACTATGCTTGAGAATATATTAGTTAGCAAGTGATTTGAAATTTTACATTCTCTCCCCGATTCACTGAAAATATCATTTTGAGGAACATAGGGAAGGAGGCAAGCAGCTATTACTGGCATGGAAGAGACAGCTCTACAATTCGTGAACTAAACCCTAAGGTTTTTTATTCTGAACTCTCGCTGCCTTACTTTGTTGGTGACTGAGAAAATAGTGCCCCTTCATCACTTCGTACATTTTCATTTACAAATAAACATATCAATTTATTTACTTTCTCGTAAAGTTTTTGTAGGCAAGTATCTCAAGAAGTCCTTGAGACCTCTCTTGTTGCGGCTTTTTATTTGGTGTAAAATTATTGCTCCTTTTAACAACTCTAGCTCTTTTTTTAATATTTTTTTTTTTTAGTTTCTTGTTTCATTTCATTTAAAATTTTTGAAAAAAAATGAGTGAATTTTAGAAATTGTCTGTAGTGTTCTAGTTCTCTTGTTGGTATGCGACGAGGTGATATTGAAGGGATAACCTTGTTTGAAATCAACTCTTTTCTGCATGGTAATGGGTCTCGTGGTTGTCAAAATTGAATTTTTCACCATAGGTGCTGGGAAGAACTGGGGAGTTCATTCTTGGTTCAACTATTTAAAAGTAAAGTAAATAAAGGAAACTAAAGTAGATTAGTTATGCGTTTAAGTCAAGTTTGGTGTTTGTAGACATTCAGAGACAACCATCCAAACTGGAGCTCTGTATGGGGGAAACAATCAGCAGGTCTTAATTAAAAGAATAAGGGAATTTCTCTGGATCTTTGTGTTAGATTGATATGATGTTAAATTTGTCTTCACCCATTAGCTTAAGCTTTTGGATCAATAAATGATTTAACACGATATTACAAAAGTGTATTTCTCTTTTCAACAACATCCTAGATTTGACCAATTAAGGTTTAGATGATCTTCAATTGTTCACACTTGTAGACACTAATGTCTGGATTGGATGATTATCCACGTGCTTCACACCCCACTGAAGATGAGCGTCATGTGGACCTCCGATGCTGGATGCTTCTCGCTGCAGATTGCATGCATTCTATTTCAGAGCTAACCGCAAAGGAAAAGGGACTTGAAACGGTAAATTGTTATGATCAACTTAGCTTTCATTTATGTTTTTTCCTTTATATTTCCCCTCAAAAGTTTTGAAACTTCATGACATTGCAGGTTTATAGTTCAGCAACAAAAATTCTCTCTGAATTTGAGCTTCTGAATCAGGTTGGTTGTCACTTTCATCTCTGCTGGAAAATTGTTTTACAATGTATGGAGATGATTATTTATCCAAGTGGTCTTTTCTGTTGAACATTTGAAGTGGTAATAGCTAATAAGCTCCAGACCTCTAACTTGACTATGATTGATGCCTGCCAGATGCACTTTGATGATGCCCATGGAACTTACTTAGATTTTGGAAATCACACTGAAAAGGTACGGTCTCTTTGTTACCATCGTCTTTACCATACGACTTTCATTTAGTTATCTCCCAGAAATCAACTTTCAATGTGTGAGTGTACTTTTCTCTACTTTTATGTATGTCGATTGGAATACTCCCATGATTTCTCACTCGCTCTCTTGCACTTTCCTGATTTTTTTAATATATCCCGCAACGAATTTGGCTTTTGATGAGTTTGTAAAGTTGTTTCCTTTATTAATAGATAAATTACAAAACGAACAGTAACCCAAAGGCTCCCAAACTCCATCAACTAAATAACTTGGCACTGAAATTTTCAATTGCACTGATGGAAATTTATACCTTAAAAAATTGTCCAATCTTGTGTTCTTAATGCAAGAATTGCTTTCGTGTAGGTACGTTTAATTTGGAAAGAGGTGATGGGAGAGCAAGGATTTACAACTCGTCAACTTATCCGGGAAGTGTCGGAAACGCCAAGGCTAAGAATGGTTCCTCATATCGGTTATGTCAGTCTTTTTCCACTCATGGGGAGAATCATTCCACCTGTAAGTGTTAGCAACATGAAACCACCATTCATATGCTTATTTTCCTGTCGCTAAGGGGCTTGATCATTTTTTTTCTTCATCTCTCCTACAGGAATCTTGGATTCTTGAAAAACAGCTTGATCTTATTTCAAATCGAAGCATATTCTGGACAGATTATGGGCTCCGCTCCCTATCTAAAACAAGGTACCTTTACATTATCTTCTTTTTTAAAAGAAATCATGGTTTTCAATTGATGAAAAGTAAAAAACTTCCACGGAATCACAACACTAAACGGTTTGGGTTTGTTGATGAACATATATTAACAAATAGAAGTTATGATGCCATGTATCCTAGCTTCCATTTCTTTTGATGAACCCGAACCCAAAAACGTACAATGTATAGGTATGTGGATTAATTGGGTTTTTGAAATTGTATGTTGTGTAGCTCATTATACATGAAGCACAACACAGAGCATGATGCACCTTATTGGAGAGGTACAATTTGGATGAACATGAATTACTTGATTCTTTCAGCACTCAATCACTACGCCACAGGTATTTTCAATCCATCGGATCCCTTTGAAAATCTCATGTGAGCATCTTTTTTTCTTTTGGCTAGTGCCTTTTAACGTGACTTGAATGGTGCAGAGCATGGACCGTATAGAGAAAAAGCTAAAGATATTCGTGATGAGTTGAGAAGCAATATAATTAGGTAATTACAACTCTTTTTTCCCCATTCTTTTAAGTTCTGTCTTCGGATGATTATACGCTTCCCTTATTATTTTCTTTTATTTTTTATTTTATAACTTTTTTCAATATAGCAAAATGAATCAAAATGTTTATAAAACTATCTGTCAATCTGCAATGAACCACGATATACTCAGATAATCCAAATCGAACCATGAACAGTAATACTGAAAGCAGACATGTTAATAATTTGTGCAGAAATGTGGTTAGAAATTATCAGAAGACAGGATACATTTGGGAGCAGTATAATCAAAAGACGGGTAAAGGGAAAGGCGCTCACCCATTTACCGGTTGGACATCTCTACTACTCTTAATCATGGCTGAAGCTTATACAGAAAGTTAGCAGAAGCTTCTTTGGTATTCATATCCCATCCCTCCTCACGTAATCTTCACATTTTTTTGTTGTTTTAACCCGTTCCCTTGCATGATATCTGTGGTCGATCATATAATTTTATGGACACAACTTAGTACATCTTAACTCTTCTGGGCGCAGGAAACATTTTTTATGACTGTGGAACTACAGGAGAGAGTGCCTGCAAGGAAAGAGTGGACTTTATCTCGGTACGTTGGTATGTTAGTGATACGTAGGAGCATTTATTTATCATAGATTTATTTGAGAATTCCATCATATTACAATTTTACTTGCCTAATCTCTTCTTTTTTTTTAGTAACTATTCTATTTTTAGTTTTTGAAGATTATCGTCTAAAATAATTTCAAAGTTTGAGTCAAATTTTGGAAGCTAGTTTAAGAAAAAATTGTTTTTATAAATTAGTAAAAATGAAAATCATTACATTATAAATAATTGCAGGTTTTATAAAAGAAAAAATTTGTTATTAGGTCAATTAATTTTTTCTAAAGTGAAATAACAAATGCTTTTCTCATTGGCCTTCTGAAATATCACATTGACAAACGCACTTGATATTATCATCAAGATTTAGAATTAATTCTGTTCTACTCATTCGTACGTTCTTCTCACGATTGTAAAAATTACAAACCCACTAAATAAAAATGGTAAAAGCACTTTCGACAAATTTCATCTTTTCGATCTTTTTATTAAATCCTTGGGTCCTTTGATATGCTGTAATTTATTTATTTTTCAAATTCCAATTATCGAATTTTAATTGGATACGTAATTGAAGGTATCAATAATAAAATTCAATCCAATAATTAAACTGAATGAGACCCAAGAGAATAAATCTATGGTTTGACCTAATAGTTTAAATGATGCAAGTTATAAATCCTAGTTTGAAAAATAAAAACAATACCCAAAACTCAATTATAAGCAAACATCCAACAAGGTTTAATGTCAAAACGTTATTTTAATTACTAAATTTCAAAACATTTTCAATTACATAAGAAAATCTATAAACAAAAAAAAAAGCGTAAATCTTAATTGATTAAATATTTTATTATATTTATAGACACTTTTAAACAGTTCTGTAATTTAAAACAAAATTTTACTATCTCTTTGACCTCTTAACTTTCCCTCTAAAGTCAAATCTTAAGAAAAGTTCTTTAGAATACGTGCTACCAAAATAAATAATAATTTATTATACGAGTTCTAATTTTAAATAAAATTAAAAAGAATCGATTTAAAAAAGAATCGATATTTACTATATATTCTAACTTAAAAACCCTTAACGTTTGTCAATCCAATATTTCCACAACATTGCCTCGATGGAAACGAATTCAAATTCCCACAATTCTTGTCTAGAAATGGCCCTATTATATCCCAATCTAATCTTATAGTGATTGAAAGTTTCAATTATTAATACTCACTTATACTTCACTAAAATTATTTGAGCTCTCTACTGATTTTGTAACTCAATCTGAAGTAAAACTACATCCCAAACACATTCTACAGTAACTACTAACTTTCAAAGAATTGCTCATCTCTCCCACCCTTTGTAGTTGCTCTCATGTTAAGAACACCTAACAACTAAATATACTATACGTTACCCCCTTTTTCAAAAGCTTTTTAGATGATAGAATAAGAGAGGTAACACTCCCAAATGGTAAGATTTTAAAGTGAGGCCATCTCACGCACCTGACAAGGGGGAAAGATGAATCCAAGGCCTTGCATGACGATGCTGAGTACTTTAAGAGCCACTACAGTTCTCAGCCTTTAAACACTGCATCAAATCAAAATTGCATAGGTATTAAGCAGTTAAAAGTTTAACCTATCTTAACCGAACTGTACTAACACACTTCTCTCTATAAGATGTGGGATAGCACCCTTGAGCAGACTAGTACATAAAAGTTTTACAGAAACGAATTTTAACTTTTCCATTTGGAAAAATAATCAAGCCAAGTATACTTTAATAACGAACAACACAGCAGAAGAATGGCTAGAAATGGGAAACTCAATCTCTTTCAACTTGCATCATCTGCCATCTCTTTATCTTCAGCCATTATGATTTCCATGTCTCTAACATCTCGCTTTAAAACATTTCCAAGTCTAGCAATGGCCTCAGTCTGCTGCTGTAAAACCTGTCACACAACATGGAGCCGTGGCATACACAAAGTTGGTTAATCAAAATTTTCACTTATATGATCATTACACCGTGACTTCTTTTTGAAAGACTGTTTATAGAACTTTAGTAAACTTCACAATGTTTATTTCCAGCAAAACTAGAAGTGATAAAGCAGGCTCCAAGCAAAAATATGTTCGCACCTCCTGCATATCGGCAAGACTCTGCTCGTGTATTTTTGCAGATCCTTGAAAATAGATGGAATTACCAGGACCGGAGCCGTTTGCTTGCCCACGAGTAACTGTCAGCAAATTTTGTACCCTCCTTGAAAGTTCTGCCCCAGAGCCTTTCAACTG

Coding sequence (CDS)

ATGTGTGGAGGGTCTAAGTCGCATTTCTTATTTCGTAAGTTTACATTGTTTGGACTTGTCGATTATCCACAAGAAGGGTTATTATTTTTCTTGGCTTCCATGCTTCACGTATTATTTTTGTTCCCACTTCAATTAGGTTCATCATTAACTGATCGATTAAAGGAGAAAGAATTAGAATTTGATACCAGATTTGAGGAGTGCTTTGGTTTGGCAAACAAGTTTGATTTAGAATCCACAACTGTTGGTAAGGCTGCTGTTTCAAACTTATTAGGTGGCATTGGTTATTTCTATGGCCAGTCAAAAATTGCACTCCCAGGTGCCTCCCATCTCGGAAGTCATAATGGTTTCCTTTTTTACTGGCCTGCTGAGCTTTACACTGCTGTTCCATGCCGTCCTGTCTTCCCTAGGGGATTCTTGTGGGATGAAGGCTTTCATCAGCTGCTTATCTGGCGATGGGATATTCACATCAGCTTAGATATTCTTGGTCACTGGTTGGATTTAATGAACATCGATGGATGGATTCCACGTGAACAAATATTAGGGGCTGAAGCTCTCAGCAAGGTCCCAGAAGAATTTATTCCTCAGTATCCAAGCAATGCTAACCCGCCAGCATTCTTTCTAGTCTTGCGAGAATTGATTCATGGCCTAAAGAAAAATGCATTTACCGAGACAGAAAGCTCAGAGATATCTTCATTCTTCGAACGGTCTTTTGTCCGCTTGGAAGCATGGTTCCAGTGGTTTAATACTACACAGCCAGGGAAGGAGGCAAGCAGCTATTACTGGCATGGAAGAGACAGCTCTACAATTCGTGAACTAAACCCTAAGACACTAATGTCTGGATTGGATGATTATCCACGTGCTTCACACCCCACTGAAGATGAGCGTCATGTGGACCTCCGATGCTGGATGCTTCTCGCTGCAGATTGCATGCATTCTATTTCAGAGCTAACCGCAAAGGAAAAGGGACTTGAAACGGTTTATAGTTCAGCAACAAAAATTCTCTCTGAATTTGAGCTTCTGAATCAGATGCACTTTGATGATGCCCATGGAACTTACTTAGATTTTGGAAATCACACTGAAAAGGTACGTTTAATTTGGAAAGAGGTGATGGGAGAGCAAGGATTTACAACTCGTCAACTTATCCGGGAAGTGTCGGAAACGCCAAGGCTAAGAATGGTTCCTCATATCGGTTATGTCAGTCTTTTTCCACTCATGGGGAGAATCATTCCACCTGAATCTTGGATTCTTGAAAAACAGCTTGATCTTATTTCAAATCGAAGCATATTCTGGACAGATTATGGGCTCCGCTCCCTATCTAAAACAAGCTCATTATACATGAAGCACAACACAGAGCATGATGCACCTTATTGGAGAGGTACAATTTGGATGAACATGAATTACTTGATTCTTTCAGCACTCAATCACTACGCCACAGAGCATGGACCGTATAGAGAAAAAGCTAAAGATATTCGTGATGAGTTGAGAAGCAATATAATTAGAAATGTGGTTAGAAATTATCAGAAGACAGGATACATTTGGGAGCAGTATAATCAAAAGACGGGTAAAGGGAAAGGCGCTCACCCATTTACCGGTTGGACATCTCTACTACTCTTAATCATGGCTGAAGCTTATACAGAAAGTTAG

Protein sequence

MCGGSKSHFLFRKFTLFGLVDYPQEGLLFFLASMLHVLFLFPLQLGSSLTDRLKEKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHNGFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTSLLLLIMAEAYTES
Homology
BLAST of Cucsat.G9991 vs. ExPASy Swiss-Prot
Match: F4HTM3 (Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS1 PE=1 SV=1)

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 549/855 (64.21%), Postives = 664/855 (77.66%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M G SRR+ R RIKS+S     D+ +    S      R + K   SI  F  NLK++VG+
Sbjct: 1   MTGASRRSARGRIKSSSLSPGSDEGSAYPPSIR----RGKGKELVSIGAFKTNLKILVGL 60

Query: 61  CFVAFFVILYLISSLIVTG----DHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRP 120
             +   VI ++I+ L+  G    + Q PRVITPFPAPKV DL  FQGEHKESLYWGTYRP
Sbjct: 61  IILGIIVIYFVINRLVRHGLLFDESQKPRVITPFPAPKVMDLSMFQGEHKESLYWGTYRP 120

Query: 121 HVYLGIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVD 180
           HVY G+RARTP SL+AGLMW+GV++  YVMRH C++SD+LST+GW  HN RD+G Q LV+
Sbjct: 121 HVYFGVRARTPLSLVAGLMWLGVKDEMYVMRHFCENSDDLSTFGWREHNGRDYGRQELVE 180

Query: 181 QDMTLGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSL 240
            DM + TSF+KSK  G GYGGDWAVRI V +K     +++ ++ HLFFYLADE GN L+L
Sbjct: 181 NDMVIETSFVKSKGDGLGYGGDWAVRIDVKNKGL--NDDVKRSAHLFFYLADEGGNVLNL 240

Query: 241 SRDAMNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQ 300
            +D ++   +SLL SGSR DVG+WQ+HL+S++ LE H+SGFKT H +NLS+LVQ+N+  Q
Sbjct: 241 GQDGLDFQGSSLLVSGSREDVGDWQIHLKSQNQLETHYSGFKTPHIYNLSDLVQQNLALQ 300

Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLT 360
           ARKFGRLQLSDTS+DSSNI +FQISGR+PF  D+ FISG    SS  E+R+ +LTG  L+
Sbjct: 301 ARKFGRLQLSDTSEDSSNIYIFQISGRLPFTIDIPFISGIKGESSNVEKRLTSLTGLPLS 360

Query: 361 DRLKEKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHL 420
           D LK+K  EFD +F ECF L+ K D E+  VG+ A++N+LGGIGYFYGQSKI +P ++  
Sbjct: 361 DLLKKKHQEFDAKFNECFKLSEKHDSETLGVGRTAIANMLGGIGYFYGQSKIYVPKSTQP 420

Query: 421 GSHNGFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI 480
           GS + FL YWPAELYTAVP RP FPRGFLWDEGFHQLLIWRWDI I+LDI+GHWLDL+NI
Sbjct: 421 GSRDNFLLYWPAELYTAVPSRPFFPRGFLWDEGFHQLLIWRWDIRITLDIVGHWLDLLNI 480

Query: 481 DGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEIS 540
           DGWIPREQILGAEALSKVPEEF+ QYPSN NPP  FLV+R+LI  ++   F  +E  E+ 
Sbjct: 481 DGWIPREQILGAEALSKVPEEFVVQYPSNGNPPTLFLVIRDLIDAIRMEKFVASEKDEVL 540

Query: 541 SFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHP 600
           SF ER+ VRL+AWFQWFNT+Q GKE  SY+WHGRD++T +ELNPKTL SGLDDYPRASHP
Sbjct: 541 SFLERASVRLDAWFQWFNTSQKGKEIGSYFWHGRDNTTTQELNPKTLSSGLDDYPRASHP 600

Query: 601 TEDERHVDLRCWMLLAADCMHSISELTAKEKGL-ETVYSSATKILSEFELLNQMHFDDAH 660
           +EDERHVDLRCWM LAADCMHSI+EL  KE  L +  Y+S  K+LS F LLNQMH+D  +
Sbjct: 601 SEDERHVDLRCWMYLAADCMHSITELLGKEDKLSKENYNSTVKLLSNFNLLNQMHYDSDY 660

Query: 661 GTYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRII 720
           G Y DFGNHTEKV+LIWKEV+ E G  +RQL+R+    P+L++VPH+GYVS FP M RII
Sbjct: 661 GAYFDFGNHTEKVKLIWKEVIQENGQLSRQLVRKTFGKPKLKLVPHLGYVSFFPFMSRII 720

Query: 721 PPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLI 780
           PP+S ILEKQLDLISNRSI W+DYGL SL+KTSS+YMK NTEHDAPYWRG IWMNMNY+I
Sbjct: 721 PPDSPILEKQLDLISNRSILWSDYGLVSLAKTSSMYMKRNTEHDAPYWRGPIWMNMNYMI 780

Query: 781 LSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPF 840
           LS+L HY+   GPYREK+K I  ELRSN+IRNVVRNY +TGYIWEQY+Q  G GKG   F
Sbjct: 781 LSSLYHYSIVDGPYREKSKAIYTELRSNLIRNVVRNYYETGYIWEQYDQVKGTGKGTRLF 840

Query: 841 TGWTSLLLLIMAEAY 851
           TGW++L LLIM+E Y
Sbjct: 841 TGWSALTLLIMSEDY 849

BLAST of Cucsat.G9991 vs. ExPASy Swiss-Prot
Match: Q84M89 (Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 2.9e-303
Identity = 483/776 (62.24%), Postives = 608/776 (78.35%), Query Frame = 0

Query: 76  IVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYLGIRARTPRSLLAGLM 135
           ++  + Q PRVITPFP+PK+TDL  FQGEHKESLYWGTYRP +Y G+RARTP+SL+AGLM
Sbjct: 13  LLDDESQRPRVITPFPSPKLTDLSMFQGEHKESLYWGTYRPQMYFGVRARTPKSLVAGLM 72

Query: 136 WIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMTLGTSFLKSKESGSGY 195
           W+ +++G+ VMRH C++S++L ++GW  HN RDFG Q L +Q+M L TSF+KS+E   GY
Sbjct: 73  WLVMKDGKPVMRHFCENSNDLKSFGWKEHNGRDFGRQELFEQNMVLETSFVKSEEGSLGY 132

Query: 196 GGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDAMNIHETSLLASGSRS 255
           GGDW++RI V +  ++  +E+ ++ HLFFYLADE  N ++L +D + + E+S+LASGSR 
Sbjct: 133 GGDWSIRINVQNMLND--DEVKRSTHLFFYLADEGCNGVNLGKDVLRLKESSVLASGSRQ 192

Query: 256 DVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKFGRLQLSDTSDDSSNI 315
           DVGNWQ+HL+S++ LE H+ GFKT    NLS LVQ+N+  Q  K G LQLSD+S+DSSNI
Sbjct: 193 DVGNWQMHLKSQNHLETHYCGFKTPDIVNLSGLVQQNLTAQEEKSGLLQLSDSSEDSSNI 252

Query: 316 LVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLKEKELEFDTRFEECFG 375
            VFQIS       D+AF+SG    +S  E R+ +LTG  L+  L+EK + F+ +F ECF 
Sbjct: 253 YVFQISTTNQSTIDIAFVSGIKEETSNMENRIMSLTGLPLSSLLEEKHIAFNAKFNECFN 312

Query: 376 LANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHNGFLFYWPAELYTAVP 435
           L++K D E+  VGKAA+ N+LGGIGYFYGQSKI  P +    S + FL YWPAELYTAVP
Sbjct: 313 LSDKLDPETLVVGKAAIGNMLGGIGYFYGQSKIHFPKSPLAKSEDDFLLYWPAELYTAVP 372

Query: 436 CRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWIPREQILGAEALSKVP 495
            RP FPRGFLWDEGFHQLLIWRWD+HI+L+I+G+WLDLMNIDGWIPREQ+LGAEALSK+P
Sbjct: 373 SRPRFPRGFLWDEGFHQLLIWRWDVHITLEIVGNWLDLMNIDGWIPREQVLGAEALSKIP 432

Query: 496 EEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFERSFVRLEAWFQWFNT 555
           ++++ Q+PSN NPP   LV+R+LI+G++   F E +  +I SF +R+FVRL+AWF+WFNT
Sbjct: 433 KQYVVQFPSNGNPPTLLLVIRDLINGIRTEKFNEADRDKILSFLDRAFVRLDAWFKWFNT 492

Query: 556 TQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADC 615
           +Q GKE  SYYWHGRD+ T RELNP++L SGLDDYPRASHP EDERHVDLRCWM LAAD 
Sbjct: 493 SQIGKEKGSYYWHGRDNITNRELNPQSLSSGLDDYPRASHPNEDERHVDLRCWMYLAADS 552

Query: 616 MHSISELTAKEKGLETV-YSSATKILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKE 675
           M+SI E   K   L T  YSS  K+LS+F LLNQMH+D  HG YLDFGNHTE+VRL+WKE
Sbjct: 553 MNSIQEFMGKNDILVTEDYSSIAKLLSDFTLLNQMHYDKDHGAYLDFGNHTEEVRLVWKE 612

Query: 676 VMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESWILEKQLDLISNRSI 735
           V+ E G  +R+L+RE    P LR+VPHIGYVS FP M RIIP +S IL+KQLDLISN +I
Sbjct: 613 VIHEDGHLSRELVRETYGKPELRLVPHIGYVSFFPFMFRIIPADSSILDKQLDLISNGNI 672

Query: 736 FWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALNHYATEHGPYREKAK 795
            W+DYGL SL+KTSSLYMK+N+EH APYWRG IWMNMNY+ILS+L+HY+T  GPY  KA+
Sbjct: 673 VWSDYGLLSLAKTSSLYMKYNSEHQAPYWRGAIWMNMNYMILSSLHHYSTVDGPYNSKAR 732

Query: 796 DIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTSLLLLIMAEAY 851
            I +ELRSN+IRNVVRNY +TG IWE Y+Q  G G+GA  FTGW++L+LLIM+E Y
Sbjct: 733 TIYEELRSNLIRNVVRNYDQTGIIWEHYDQTKGTGEGARVFTGWSALILLIMSEEY 786

BLAST of Cucsat.G9991 vs. ExPASy Swiss-Prot
Match: Q80UM7 (Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 8.6e-154
Identity = 312/795 (39.25%), Postives = 434/795 (54.59%), Query Frame = 0

Query: 85  RVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYLGIRARTPRSLLAGLMWI--GVQNG 144
           R +T  PAP               L+WGTYRPHVY G++ R+P+ LL GLMW   G   G
Sbjct: 68  RALTLHPAPSALPPDSSSPAVAPELFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127

Query: 145 R-YVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMTLGTSFLKSKESGSGYGGDWAV 204
               +RH C+  D +  YGW  H+ R FG Q + D  + L T F+  K  G  +GGDW+ 
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGRTFGRQHIHDGALRLTTEFV--KRPGGQHGGDWSW 187

Query: 205 RIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDAMNIHETSLLASGSRSDVGNWQ 264
           R+ V  + S      L +  LFFY+   DG  + L          S+  SG  S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPEIGAKGQLKSI--SGHTSELGDFR 247

Query: 265 LHL---ESKDDLEVHFSGFKTEHYHN-----LSELVQENI---------GGQARKF---- 324
           L L    S  D       +      N     L+++V+  +         G    ++    
Sbjct: 248 LTLLPPTSPGDTVPKHGSYNVFWSSNPGLPQLTDMVKSRLNSWFQHRPPGASPDRYLGLP 307

Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRL 384
           G L+  +         L+ Q++ + PF  +  F SGS      +      L GS LT  L
Sbjct: 308 GSLKWEERGPSGQGQFLIQQVTLKAPFSVEFVFESGSAATGGNQAS--GRLVGSQLTQAL 367

Query: 385 KEKELEFDTRFEECFGLANK-FDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGS 444
           +     F  RFE+ F L  K    E   +G+ A+S LLGGIGYFYGQ  + LP  S  GS
Sbjct: 368 ESHAAAFKERFEKTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTSMEGS 427

Query: 445 HNGF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI 504
                   + P  L++ VP R  FPRGFLWDEGFHQL++ RWD H++ + LGHWL L+N 
Sbjct: 428 EQKMDPALFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVVQRWDPHLTREALGHWLGLLNA 487

Query: 505 DGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEIS 564
           DGWI REQILG EA ++VP EF+ Q  ++ANPP   L +  ++ G            +  
Sbjct: 488 DGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVVHMLEG---------HDPDDL 547

Query: 565 SFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHP 624
           +F  ++F RL AWF W + +Q G    SY W GRD +    LNPKTL SGLDDYPRASHP
Sbjct: 548 AFLRKAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASHP 607

Query: 625 TEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHG 684
           +  ERH+DLRCW+ L A  +  ++E   + +    +   A   L E   L+++H+    G
Sbjct: 608 STAERHLDLRCWVALGARVLSQLAEQLGETEAAAELGPLAAS-LEEPGSLDELHWAPELG 667

Query: 685 TYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSE-TPRLRMVPHIGYVSLFPLMGRII 744
            + DFGNHT+ V+L  +   G        L+R V    PRL+ V  +GYVSLFPL+ +++
Sbjct: 668 VFADFGNHTKAVQLKSRPPQG--------LVRVVGRPPPRLQYVDALGYVSLFPLLLQLL 727

Query: 745 PPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLI 804
            P S  L   LD++++    W+ +GLRSLS +S  Y + NTEHD PYWRG +W+N+NYL 
Sbjct: 728 DPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYLA 787

Query: 805 LSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPF 851
           L AL+HY    GP++ +A  +  ELR+N++RNV + YQ TG++WEQY+ + G+G G  PF
Sbjct: 788 LGALHHYGHVEGPHKVQAAKLYHELRANVVRNVRQQYQATGFLWEQYSDQDGRGMGCRPF 834

BLAST of Cucsat.G9991 vs. ExPASy Swiss-Prot
Match: O88941 (Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.3e-151
Identity = 308/795 (38.74%), Postives = 431/795 (54.21%), Query Frame = 0

Query: 85  RVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYLGIRARTPRSLLAGLMWI--GVQNG 144
           R +T  PAP                +WGTYRPHVY G++ R+P+ LL GLMW   G   G
Sbjct: 68  RALTLHPAPSALPPDSSSPAVAPEFFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGATPG 127

Query: 145 R-YVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMTLGTSFLKSKESGSGYGGDWAV 204
               +RH C+  D +  YGW  H+   FG Q + D  + L T F+  K SG  +GGDW+ 
Sbjct: 128 TPPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIYDGALRLTTEFV--KRSGGHHGGDWSW 187

Query: 205 RIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDAMNIHETSLLASGSRSDVGNWQ 264
           R+ V  + S      L +  LFFY+   DG  + L    +         SG  S++G+++
Sbjct: 188 RVTVEPQASGTPSFPLVS--LFFYVV-TDGQEVLLPE--VGAKGQLKFISGHTSELGDFR 247

Query: 265 LHL---ESKDDLEVHFSGFKTEHYHN-----LSELVQENI---------GGQARKF---- 324
           L L    +  D       +      N     L+++V+ ++         G    ++    
Sbjct: 248 LTLLPPTTPGDTVPKHGSYNVFWSSNPGLPLLTDMVKSHLNSWFHHRPPGASPERYLGLP 307

Query: 325 GRLQLSDTS-DDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRL 384
           G L+  +         LV Q++ + PF  +  F SGS    +  ++    L G  LT  L
Sbjct: 308 GSLKWEERGPSGQGQFLVQQVTLKAPFSVEFVFESGSA--RTGRDQASEQLVGGQLTRAL 367

Query: 385 KEKELEFDTRFEECFGLANK-FDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGS 444
           +     F  RFE  F L  K    E   +G+ A+S LLGGIGYFYGQ  + LP     GS
Sbjct: 368 ESHAAAFKERFERTFQLKEKGLSPEEQALGQVALSGLLGGIGYFYGQG-LVLPDTGMEGS 427

Query: 445 HNGF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI 504
                   + P  L++ VP R  FPRGFLWDEGFHQL++ RWD H++ + LGHWL L+N 
Sbjct: 428 EQKMDPSLFPPVPLFSGVPSRSFFPRGFLWDEGFHQLVVQRWDPHLTREALGHWLGLLNA 487

Query: 505 DGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEIS 564
           DGWI REQILG EA ++VP EF+ Q  ++ANPP   L +  ++ G          + E  
Sbjct: 488 DGWIGREQILGDEARARVPPEFLVQRAAHANPPTLLLPVIHMLEG---------RAPEDL 547

Query: 565 SFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHP 624
           +F  R+F RL AWF W + +Q G    SY W GRD +    LNPKTL SGLDDYPRASHP
Sbjct: 548 AFLRRAFPRLHAWFSWLHQSQAGPVPLSYRWRGRDLALPTLLNPKTLPSGLDDYPRASHP 607

Query: 625 TEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHG 684
           +  ERH+DLRCW+ L A  +  ++E   + +    +   A   L     L+++H+    G
Sbjct: 608 SAAERHLDLRCWVTLGARVLSQLAEELGETEAAAELGPLAAS-LEAAGSLDELHWAPELG 667

Query: 685 TYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETP-RLRMVPHIGYVSLFPLMGRII 744
            + DFGNHT+ V+L  +   G        L+R V   P RL+ V  +GYVSLFPL+ +++
Sbjct: 668 VFADFGNHTKAVQLKSRPPQG--------LVRVVGRPPARLQYVDALGYVSLFPLLLQLL 727

Query: 745 PPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLI 804
            P S  L   LD++++    W+ +GLRSLS +S  Y + NTEHD PYWRG +W+N+NYL 
Sbjct: 728 EPSSPRLGPLLDVLADSRHLWSPFGLRSLSASSLFYKQRNTEHDPPYWRGAVWLNINYLA 787

Query: 805 LSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPF 851
           L AL+HY    GP++ +A  +  ELR+N++ NV + YQ TG++WEQY+ + G+G G  PF
Sbjct: 788 LGALHHYGRVEGPHKVQAAKLYRELRANVVSNVRQQYQATGFLWEQYSDQDGRGMGCRPF 834

BLAST of Cucsat.G9991 vs. ExPASy Swiss-Prot
Match: Q13724 (Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5)

HSP 1 Score: 535.8 bits (1379), Expect = 8.9e-151
Identity = 311/801 (38.83%), Postives = 435/801 (54.31%), Query Frame = 0

Query: 81  HQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYLGIRARTPRSLLAGLMWI--G 140
           ++  R +T   AP V             L+WGTYRPHVY G++ R+P+ LL GLMW   G
Sbjct: 66  YRARRAVTLHSAPPVLPADSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQG 125

Query: 141 VQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMTLGTSFLKSKESGSGYGGD 200
              G   +RH C+  D +  YGW  H+   FG Q + D  + L T F+  K  G  +GGD
Sbjct: 126 TTPGTPKLRHTCEQGDGVGPYGWEFHDGLSFGRQHIQDGALRLTTEFV--KRPGGQHGGD 185

Query: 201 WAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDAMNIHETSLLASGSRSDVG 260
           W+ R+ V  + S  +   L    LFFY+   DG  + L    +         SG  S++G
Sbjct: 186 WSWRVTVEPQDSGTS--ALPLVSLFFYVV-TDGKEVLLPE--VGAKGQLKFISGHTSELG 245

Query: 261 NWQLHL---ESKDDLEVHFSGFKTEHYHN-----LSELVQENI---------GGQARKF- 320
           +++  L    S  D    +  +      N     L+E+V+  +         G    ++ 
Sbjct: 246 DFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEMVKSRLNSWFQHRPPGAPPERYL 305

Query: 321 ---GRLQLSD---TSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSS 380
              G L+  D   +       L+ Q++ +IP   +  F SGS    +   + +  L GS 
Sbjct: 306 GLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEFVFESGSA--QAGGNQALPRLAGSL 365

Query: 381 LTDRLKEKELEFDTRFEECFGLANK-FDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGA 440
           LT  L+     F  RFE+ F L  K        +G+AA+S LLGGIGYFYGQ  + LP  
Sbjct: 366 LTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQAALSGLLGGIGYFYGQG-LVLPDI 425

Query: 441 SHLGSHNGF--LFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWL 500
              GS        + P  L+TAVP R  FPRGFLWDEGFHQL++ RWD  ++ + LGHWL
Sbjct: 426 GVEGSEQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVVQRWDPSLTREALGHWL 485

Query: 501 DLMNIDGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETE 560
            L+N DGWI REQILG EA ++VP EF+ Q   +ANPP   L +  ++         E  
Sbjct: 486 GLLNADGWIGREQILGDEARARVPPEFLVQRAVHANPPTLLLPVAHML---------EVG 545

Query: 561 SSEISSFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYP 620
             +  +F  ++  RL AWF W + +Q G    SY W GRD +    LNPKTL SGLDDYP
Sbjct: 546 DPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRDPALPTLLNPKTLPSGLDDYP 605

Query: 621 RASHPTEDERHVDLRCWMLLAADCMHSISE-LTAKEKGLETVYSSATKILSEFELLNQMH 680
           RASHP+  ERH+DLRCW+ L A  +  ++E L   E   E    +A+  L   E L+++H
Sbjct: 606 RASHPSVTERHLDLRCWVALGARVLTRLAEHLGEAEVAAELGPLAAS--LEAAESLDELH 665

Query: 681 FDDAHGTYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSE-TPRLRMVPHIGYVSLFP 740
           +    G + DFGNHT+ V+L  +   G        L+R V    P+L+ V  +GYVSLFP
Sbjct: 666 WAPELGVFADFGNHTKAVQLKPRPPQG--------LVRVVGRPQPQLQYVDALGYVSLFP 725

Query: 741 LMGRIIPPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWM 800
           L+ R++ P S  L   LD++++    W+ +GLRSL+ +SS Y + N+EHD PYWRG +W+
Sbjct: 726 LLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWL 785

Query: 801 NMNYLILSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKG 851
           N+NYL L AL+HY    GP++ +A  +  ELR+N++ NV R YQ TG++WEQY+ + G+G
Sbjct: 786 NVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRG 837

BLAST of Cucsat.G9991 vs. NCBI nr
Match: XP_004135311.1 (mannosyl-oligosaccharide glucosidase GCS1 [Cucumis sativus] >KGN51681.1 hypothetical protein Csa_008593 [Cucumis sativus])

HSP 1 Score: 1748 bits (4527), Expect = 0.0
Identity = 853/853 (100.00%), Postives = 853/853 (100.00%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI
Sbjct: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF
Sbjct: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN
Sbjct: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
           GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI
Sbjct: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE
Sbjct: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE
Sbjct: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           LLLLIMAEAYTES
Sbjct: 841 LLLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. NCBI nr
Match: XP_008446049.1 (PREDICTED: mannosyl-oligosaccharide glucosidase GCS1-like [Cucumis melo] >KAA0034203.1 mannosyl-oligosaccharide glucosidase GCS1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1665 bits (4311), Expect = 0.0
Identity = 810/853 (94.96%), Postives = 828/853 (97.07%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M GQSRRNPRSRIKSA AVD D D+AVRQASDNYRR RD NKS +SIRIFNVNLKVM+G 
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIV+GDHQTPRVITPFPA KVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEE EELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           +NIHETSLLASGSRSDVGNWQLHLES+D+L+VHFSGFKTEHYHNLSELVQENIG QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISG IPFRTDLAFISGSGLP+SREEERVNNLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEE FGLANKFD ESTTVGKAAVSNLLGGIGYFYG+SKIALPGASH GS +
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
            FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMN+DGWI
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNVDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSKVPEEFIPQYPSNANPPA FLVLRELIHGLKKNAFTETESSEISSF E
Sbjct: 481 PREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFLE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           R+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE
Sbjct: 541 RAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISELT K KGLETVYSSATKILSEFELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMG QGFTTRQLIREVSE PRLRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           IL KQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYRE+AKDIRDELRSNIIRNVVRNY K+GY+WEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           L+LLIMAEAYTES
Sbjct: 841 LVLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. NCBI nr
Match: TYK15717.1 (mannosyl-oligosaccharide glucosidase GCS1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1652 bits (4278), Expect = 0.0
Identity = 810/875 (92.57%), Postives = 828/875 (94.63%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M GQSRRNPRSRIKSA AVD D D+AVRQASDNYRR RD NKS +SIRIFNVNLKVM+G 
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIV+GDHQTPRVITPFPA KVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEE EELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQ---- 300
           +NIHETSLLASGSRSDVGNWQLHLES+D+L+VHFSGFKTEHYHNLSELVQENIG Q    
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQDFIY 300

Query: 301 ------------------ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGL 360
                             ARKFGRLQLSDTSDDSSNILVFQISG IPFRTDLAFISGSGL
Sbjct: 301 LCCDGHWTNMSLHYLFDQARKFGRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGL 360

Query: 361 PSSREEERVNNLTGSSLTDRLKEKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGG 420
           P+SREEERVNNLTGSSLTDRLKEKELEFDTRFEE FGLANKFD ESTTVGKAAVSNLLGG
Sbjct: 361 PNSREEERVNNLTGSSLTDRLKEKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGG 420

Query: 421 IGYFYGQSKIALPGASHLGSHNGFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW 480
           IGYFYG+SKIALPGASH GS + FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW
Sbjct: 421 IGYFYGRSKIALPGASHPGSQDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW 480

Query: 481 DIHISLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLREL 540
           DIHISLDILGHWLDLMN+DGWIPREQILGAEALSKVPEEFIPQYPSNANPPA FLVLREL
Sbjct: 481 DIHISLDILGHWLDLMNVDGWIPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLREL 540

Query: 541 IHGLKKNAFTETESSEISSFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL 600
           IHGLKKNAFTETESSEISSF ER+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL
Sbjct: 541 IHGLKKNAFTETESSEISSFLERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL 600

Query: 601 NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATK 660
           NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELT K KGLETVYSSATK
Sbjct: 601 NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATK 660

Query: 661 ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRM 720
           ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMG QGFTTRQLIREVSE PRLRM
Sbjct: 661 ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRM 720

Query: 721 VPHIGYVSLFPLMGRIIPPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH 780
           VPHIGYVSLFPLMGRIIPPESWIL KQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH
Sbjct: 721 VPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH 780

Query: 781 DAPYWRGTIWMNMNYLILSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYI 840
           DAPYWRGTIWMNMNYLILSALNHYATEHGPYRE+AKDIRDELRSNIIRNVVRNY K+GY+
Sbjct: 781 DAPYWRGTIWMNMNYLILSALNHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYM 840

Query: 841 WEQYNQKTGKGKGAHPFTGWTSLLLLIMAEAYTES 853
           WEQYNQKTGKGKGAHPFTGWTSL+LLIMAEAYTES
Sbjct: 841 WEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAYTES 875

BLAST of Cucsat.G9991 vs. NCBI nr
Match: XP_038892087.1 (mannosyl-oligosaccharide glucosidase GCS1 isoform X4 [Benincasa hispida])

HSP 1 Score: 1611 bits (4171), Expect = 0.0
Identity = 776/853 (90.97%), Postives = 812/853 (95.19%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M G SRRNPRSR KS SAVD DDD+AVRQ  D YRR RDR+K   SIRIFNV+LKVMVG 
Sbjct: 1   MTGHSRRNPRSRNKSVSAVDGDDDTAVRQGPDTYRRHRDRSKLRPSIRIFNVSLKVMVGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVI YLI SL+V GDH+TPRVITPFPAPKV DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61  CFVAFFVIFYLIRSLVVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPHLYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQ SDELSTYGWT HNARDFGHQV+VDQD+T
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQDSDELSTYGWTHHNARDFGHQVIVDQDLT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           L TSFLK+KESGSGYGGDW+VRI+VNSK SEE +ELLKTGHLFFYLADEDGNAL LSRDA
Sbjct: 181 LATSFLKTKESGSGYGGDWSVRIEVNSKTSEEVKELLKTGHLFFYLADEDGNALRLSRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           MNIHETSL+ASGSR D+GNWQLHLES+DD+EVHFSGFKTEH+HNLSELVQENIG QARKF
Sbjct: 241 MNIHETSLIASGSRRDIGNWQLHLESRDDVEVHFSGFKTEHFHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISG+G PSSREEERVN+LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEECF LANKFDLESTTVGKAAVSNLLGGIGYFYG+SKIALPGASH GS +
Sbjct: 361 EKELEFDTRFEECFSLANKFDLESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSRD 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
            FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSK+PEEFIPQYPSNANPPA FLVLREL+HGLKKNAFTETESS+ISSF E
Sbjct: 481 PREQILGAEALSKIPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTETESSKISSFLE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           R+FVRLEAWFQWFNTTQPGKEASS+YWHGRDSSTIRELNPK LMSGLDDYPRASHPT+DE
Sbjct: 541 RAFVRLEAWFQWFNTTQPGKEASSFYWHGRDSSTIRELNPKALMSGLDDYPRASHPTDDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMH+ISELT K K +ETVYSSA KILS+FELLNQMHFDDA+GTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHAISELTVKRKRVETVYSSAAKILSDFELLNQMHFDDAYGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPES 
Sbjct: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESP 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           IL KQLDLISNRSIFWT+YGLRSLSKTSSLYMK+NTEH+APYWRGTIWMNMNYLILSALN
Sbjct: 721 ILGKQLDLISNRSIFWTNYGLRSLSKTSSLYMKYNTEHEAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYRE+AKDIRDELRSNIIRNVV NY KTGYIWEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYRERAKDIRDELRSNIIRNVVGNYHKTGYIWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           L+LLIMAEAYTES
Sbjct: 841 LVLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. NCBI nr
Match: XP_038892086.1 (mannosyl-oligosaccharide glucosidase GCS1 isoform X3 [Benincasa hispida])

HSP 1 Score: 1606 bits (4159), Expect = 0.0
Identity = 776/857 (90.55%), Postives = 813/857 (94.87%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M G SRRNPRSR KS SAVD DDD+AVRQ  D YRR RDR+K   SIRIFNV+LKVMVG 
Sbjct: 1   MTGHSRRNPRSRNKSVSAVDGDDDTAVRQGPDTYRRHRDRSKLRPSIRIFNVSLKVMVGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVI YLI SL+V GDH+TPRVITPFPAPKV DLPQFQGEHKESLYWGTYRPH+YL
Sbjct: 61  CFVAFFVIFYLIRSLVVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPHLYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQ SDELSTYGWT HNARDFGHQV+VDQD+T
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQDSDELSTYGWTHHNARDFGHQVIVDQDLT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSK----KSEETEELLKTGHLFFYLADEDGNALSL 240
           L TSFLK+KESGSGYGGDW+VRI+VNSK    +SEE +ELLKTGHLFFYLADEDGNAL L
Sbjct: 181 LATSFLKTKESGSGYGGDWSVRIEVNSKTWFCRSEEVKELLKTGHLFFYLADEDGNALRL 240

Query: 241 SRDAMNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQ 300
           SRDAMNIHETSL+ASGSR D+GNWQLHLES+DD+EVHFSGFKTEH+HNLSELVQENIG Q
Sbjct: 241 SRDAMNIHETSLIASGSRRDIGNWQLHLESRDDVEVHFSGFKTEHFHNLSELVQENIGAQ 300

Query: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLT 360
           ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISG+G PSSREEERVN+LTGSSLT
Sbjct: 301 ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLT 360

Query: 361 DRLKEKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHL 420
           DRLKEKELEFDTRFEECF LANKFDLESTTVGKAAVSNLLGGIGYFYG+SKIALPGASH 
Sbjct: 361 DRLKEKELEFDTRFEECFSLANKFDLESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHP 420

Query: 421 GSHNGFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI 480
           GS + FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI
Sbjct: 421 GSRDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNI 480

Query: 481 DGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEIS 540
           DGWIPREQILGAEALSK+PEEFIPQYPSNANPPA FLVLREL+HGLKKNAFTETESS+IS
Sbjct: 481 DGWIPREQILGAEALSKIPEEFIPQYPSNANPPALFLVLRELVHGLKKNAFTETESSKIS 540

Query: 541 SFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHP 600
           SF ER+FVRLEAWFQWFNTTQPGKEASS+YWHGRDSSTIRELNPK LMSGLDDYPRASHP
Sbjct: 541 SFLERAFVRLEAWFQWFNTTQPGKEASSFYWHGRDSSTIRELNPKALMSGLDDYPRASHP 600

Query: 601 TEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHG 660
           T+DERHVDLRCWMLLAADCMH+ISELT K K +ETVYSSA KILS+FELLNQMHFDDA+G
Sbjct: 601 TDDERHVDLRCWMLLAADCMHAISELTVKRKRVETVYSSAAKILSDFELLNQMHFDDAYG 660

Query: 661 TYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIP 720
           TYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIP
Sbjct: 661 TYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIP 720

Query: 721 PESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLIL 780
           PES IL KQLDLISNRSIFWT+YGLRSLSKTSSLYMK+NTEH+APYWRGTIWMNMNYLIL
Sbjct: 721 PESPILGKQLDLISNRSIFWTNYGLRSLSKTSSLYMKYNTEHEAPYWRGTIWMNMNYLIL 780

Query: 781 SALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFT 840
           SALNHYATEHGPYRE+AKDIRDELRSNIIRNVV NY KTGYIWEQYNQKTGKGKGAHPFT
Sbjct: 781 SALNHYATEHGPYRERAKDIRDELRSNIIRNVVGNYHKTGYIWEQYNQKTGKGKGAHPFT 840

Query: 841 GWTSLLLLIMAEAYTES 853
           GWTSL+LLIMAEAYTES
Sbjct: 841 GWTSLVLLIMAEAYTES 857

BLAST of Cucsat.G9991 vs. ExPASy TrEMBL
Match: A0A0A0KVA6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589980 PE=3 SV=1)

HSP 1 Score: 1748 bits (4527), Expect = 0.0
Identity = 853/853 (100.00%), Postives = 853/853 (100.00%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI
Sbjct: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF
Sbjct: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN
Sbjct: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
           GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI
Sbjct: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE
Sbjct: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE
Sbjct: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           LLLLIMAEAYTES
Sbjct: 841 LLLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. ExPASy TrEMBL
Match: A0A5A7SUQ8 (Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G001990 PE=3 SV=1)

HSP 1 Score: 1665 bits (4311), Expect = 0.0
Identity = 810/853 (94.96%), Postives = 828/853 (97.07%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M GQSRRNPRSRIKSA AVD D D+AVRQASDNYRR RD NKS +SIRIFNVNLKVM+G 
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIV+GDHQTPRVITPFPA KVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEE EELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           +NIHETSLLASGSRSDVGNWQLHLES+D+L+VHFSGFKTEHYHNLSELVQENIG QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISG IPFRTDLAFISGSGLP+SREEERVNNLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEE FGLANKFD ESTTVGKAAVSNLLGGIGYFYG+SKIALPGASH GS +
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
            FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMN+DGWI
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNVDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSKVPEEFIPQYPSNANPPA FLVLRELIHGLKKNAFTETESSEISSF E
Sbjct: 481 PREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFLE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           R+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE
Sbjct: 541 RAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISELT K KGLETVYSSATKILSEFELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMG QGFTTRQLIREVSE PRLRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           IL KQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYRE+AKDIRDELRSNIIRNVVRNY K+GY+WEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           L+LLIMAEAYTES
Sbjct: 841 LVLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. ExPASy TrEMBL
Match: A0A1S3BE51 (mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103488898 PE=3 SV=1)

HSP 1 Score: 1665 bits (4311), Expect = 0.0
Identity = 810/853 (94.96%), Postives = 828/853 (97.07%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M GQSRRNPRSRIKSA AVD D D+AVRQASDNYRR RD NKS +SIRIFNVNLKVM+G 
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIV+GDHQTPRVITPFPA KVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEE EELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           +NIHETSLLASGSRSDVGNWQLHLES+D+L+VHFSGFKTEHYHNLSELVQENIG QARKF
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISG IPFRTDLAFISGSGLP+SREEERVNNLTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGLPNSREEERVNNLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEE FGLANKFD ESTTVGKAAVSNLLGGIGYFYG+SKIALPGASH GS +
Sbjct: 361 EKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGGIGYFYGRSKIALPGASHPGSQD 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
            FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMN+DGWI
Sbjct: 421 SFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNVDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEALSKVPEEFIPQYPSNANPPA FLVLRELIHGLKKNAFTETESSEISSF E
Sbjct: 481 PREQILGAEALSKVPEEFIPQYPSNANPPALFLVLRELIHGLKKNAFTETESSEISSFLE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           R+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE
Sbjct: 541 RAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISELT K KGLETVYSSATKILSEFELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRLIWKEVMG QGFTTRQLIREVSE PRLRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           IL KQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATEHGPYRE+AKDIRDELRSNIIRNVVRNY K+GY+WEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYMWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           L+LLIMAEAYTES
Sbjct: 841 LVLLIMAEAYTES 853

BLAST of Cucsat.G9991 vs. ExPASy TrEMBL
Match: A0A5D3CUY7 (Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002160 PE=3 SV=1)

HSP 1 Score: 1652 bits (4278), Expect = 0.0
Identity = 810/875 (92.57%), Postives = 828/875 (94.63%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M GQSRRNPRSRIKSA AVD D D+AVRQASDNYRR RD NKS +SIRIFNVNLKVM+G 
Sbjct: 1   MTGQSRRNPRSRIKSAPAVDGDVDTAVRQASDNYRRHRDGNKSRTSIRIFNVNLKVMLGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
           CFVAFFVILYLISSLIV+GDHQTPRVITPFPA KVTDLPQFQGEHKESLYWGTYRPHVYL
Sbjct: 61  CFVAFFVILYLISSLIVSGDHQTPRVITPFPASKVTDLPQFQGEHKESLYWGTYRPHVYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT
Sbjct: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEE EELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEEAEELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQ---- 300
           +NIHETSLLASGSRSDVGNWQLHLES+D+L+VHFSGFKTEHYHNLSELVQENIG Q    
Sbjct: 241 INIHETSLLASGSRSDVGNWQLHLESRDNLKVHFSGFKTEHYHNLSELVQENIGAQDFIY 300

Query: 301 ------------------ARKFGRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGL 360
                             ARKFGRLQLSDTSDDSSNILVFQISG IPFRTDLAFISGSGL
Sbjct: 301 LCCDGHWTNMSLHYLFDQARKFGRLQLSDTSDDSSNILVFQISGSIPFRTDLAFISGSGL 360

Query: 361 PSSREEERVNNLTGSSLTDRLKEKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGG 420
           P+SREEERVNNLTGSSLTDRLKEKELEFDTRFEE FGLANKFD ESTTVGKAAVSNLLGG
Sbjct: 361 PNSREEERVNNLTGSSLTDRLKEKELEFDTRFEESFGLANKFDFESTTVGKAAVSNLLGG 420

Query: 421 IGYFYGQSKIALPGASHLGSHNGFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW 480
           IGYFYG+SKIALPGASH GS + FLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW
Sbjct: 421 IGYFYGRSKIALPGASHPGSQDSFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRW 480

Query: 481 DIHISLDILGHWLDLMNIDGWIPREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLREL 540
           DIHISLDILGHWLDLMN+DGWIPREQILGAEALSKVPEEFIPQYPSNANPPA FLVLREL
Sbjct: 481 DIHISLDILGHWLDLMNVDGWIPREQILGAEALSKVPEEFIPQYPSNANPPALFLVLREL 540

Query: 541 IHGLKKNAFTETESSEISSFFERSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL 600
           IHGLKKNAFTETESSEISSF ER+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL
Sbjct: 541 IHGLKKNAFTETESSEISSFLERAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIREL 600

Query: 601 NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATK 660
           NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELT K KGLETVYSSATK
Sbjct: 601 NPKTLMSGLDDYPRASHPTEDERHVDLRCWMLLAADCMHSISELTGKGKGLETVYSSATK 660

Query: 661 ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRM 720
           ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMG QGFTTRQLIREVSE PRLRM
Sbjct: 661 ILSEFELLNQMHFDDAHGTYLDFGNHTEKVRLIWKEVMGAQGFTTRQLIREVSEPPRLRM 720

Query: 721 VPHIGYVSLFPLMGRIIPPESWILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH 780
           VPHIGYVSLFPLMGRIIPPESWIL KQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH
Sbjct: 721 VPHIGYVSLFPLMGRIIPPESWILGKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEH 780

Query: 781 DAPYWRGTIWMNMNYLILSALNHYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYI 840
           DAPYWRGTIWMNMNYLILSALNHYATEHGPYRE+AKDIRDELRSNIIRNVVRNY K+GY+
Sbjct: 781 DAPYWRGTIWMNMNYLILSALNHYATEHGPYRERAKDIRDELRSNIIRNVVRNYHKSGYM 840

Query: 841 WEQYNQKTGKGKGAHPFTGWTSLLLLIMAEAYTES 853
           WEQYNQKTGKGKGAHPFTGWTSL+LLIMAEAYTES
Sbjct: 841 WEQYNQKTGKGKGAHPFTGWTSLVLLIMAEAYTES 875

BLAST of Cucsat.G9991 vs. ExPASy TrEMBL
Match: A0A6J1H118 (mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC111458598 PE=3 SV=1)

HSP 1 Score: 1567 bits (4057), Expect = 0.0
Identity = 757/853 (88.75%), Postives = 798/853 (93.55%), Query Frame = 0

Query: 1   MAGQSRRNPRSRIKSASAVDDDDDSAVRQASDNYRRLRDRNKSSSSIRIFNVNLKVMVGI 60
           M G SRRNPRSRIKS+SAVD D D AVRQA D  RR RDRN     IRIF+VNLKV+VG 
Sbjct: 1   MTGHSRRNPRSRIKSSSAVDGDGDGAVRQAPDISRRHRDRNTLRPLIRIFDVNLKVLVGF 60

Query: 61  CFVAFFVILYLISSLIVTGDHQTPRVITPFPAPKVTDLPQFQGEHKESLYWGTYRPHVYL 120
            F AFFVILYLI SLIV GDH+TPRVITPFPAPKV DLPQFQGEHKESLYWGTYRP +YL
Sbjct: 61  SFGAFFVILYLIRSLIVVGDHRTPRVITPFPAPKVIDLPQFQGEHKESLYWGTYRPQLYL 120

Query: 121 GIRARTPRSLLAGLMWIGVQNGRYVMRHVCQSSDELSTYGWTRHNARDFGHQVLVDQDMT 180
           GIRARTP SLLAGLMWIGVQNGRYVMRHVCQ SDELSTYGWT HNARDFGHQVLVD DMT
Sbjct: 121 GIRARTPLSLLAGLMWIGVQNGRYVMRHVCQGSDELSTYGWTHHNARDFGHQVLVDHDMT 180

Query: 181 LGTSFLKSKESGSGYGGDWAVRIQVNSKKSEETEELLKTGHLFFYLADEDGNALSLSRDA 240
           L TSFLK+KESGSGYGGDWAVRI+VNSK+SEE +ELLKTGHLFFYLADEDGNALSL+RDA
Sbjct: 181 LTTSFLKTKESGSGYGGDWAVRIEVNSKRSEEAKELLKTGHLFFYLADEDGNALSLNRDA 240

Query: 241 MNIHETSLLASGSRSDVGNWQLHLESKDDLEVHFSGFKTEHYHNLSELVQENIGGQARKF 300
           MNIHETSLLASGSR D+GNWQLHLES+DD++VHFSGF+T H+HNLSELVQ+N+G QARKF
Sbjct: 241 MNIHETSLLASGSRRDIGNWQLHLESRDDVQVHFSGFRTGHFHNLSELVQDNLGAQARKF 300

Query: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGSGLPSSREEERVNNLTGSSLTDRLK 360
           GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISG+G PSSREEERVN+LTGSSLTDRLK
Sbjct: 301 GRLQLSDTSDDSSNILVFQISGRIPFRTDLAFISGTGFPSSREEERVNSLTGSSLTDRLK 360

Query: 361 EKELEFDTRFEECFGLANKFDLESTTVGKAAVSNLLGGIGYFYGQSKIALPGASHLGSHN 420
           EKELEFDTRFEECFGL NKFDLESTTVGKAAV+NLLGGIGYFYGQSKIALP  SH  S +
Sbjct: 361 EKELEFDTRFEECFGLGNKFDLESTTVGKAAVANLLGGIGYFYGQSKIALPDPSHPESRD 420

Query: 421 GFLFYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHISLDILGHWLDLMNIDGWI 480
            FL+YWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIH+SLDILGHWLDLMNIDGWI
Sbjct: 421 SFLYYWPAELYTAVPCRPVFPRGFLWDEGFHQLLIWRWDIHVSLDILGHWLDLMNIDGWI 480

Query: 481 PREQILGAEALSKVPEEFIPQYPSNANPPAFFLVLRELIHGLKKNAFTETESSEISSFFE 540
           PREQILGAEA+SKVPEEFIPQ+PSNANPPA FLVLREL+HGLKKNAFT TESSEISSF E
Sbjct: 481 PREQILGAEAVSKVPEEFIPQHPSNANPPALFLVLRELVHGLKKNAFTTTESSEISSFLE 540

Query: 541 RSFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTEDE 600
           R+FVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPT+DE
Sbjct: 541 RAFVRLEAWFQWFNTTQPGKEASSYYWHGRDSSTIRELNPKTLMSGLDDYPRASHPTDDE 600

Query: 601 RHVDLRCWMLLAADCMHSISELTAKEKGLETVYSSATKILSEFELLNQMHFDDAHGTYLD 660
           RHVDLRCWMLLAADCMHSISEL  K K + TVYS A K LS+FELLNQMHFDDAHGTYLD
Sbjct: 601 RHVDLRCWMLLAADCMHSISELIMKGKEVGTVYSLAAKTLSDFELLNQMHFDDAHGTYLD 660

Query: 661 FGNHTEKVRLIWKEVMGEQGFTTRQLIREVSETPRLRMVPHIGYVSLFPLMGRIIPPESW 720
           FGNHTEKVRL WKEVM EQG+TTRQLIREVS TP+LRMVPHIGYVSLFPLMGRIIPPESW
Sbjct: 661 FGNHTEKVRLSWKEVMVEQGYTTRQLIREVSGTPQLRMVPHIGYVSLFPLMGRIIPPESW 720

Query: 721 ILEKQLDLISNRSIFWTDYGLRSLSKTSSLYMKHNTEHDAPYWRGTIWMNMNYLILSALN 780
           ILEKQLDLISNRS+FWT+YGLRSLSKTSSLYMK+NTEHDAPYWRGTIWMNMNYLILSALN
Sbjct: 721 ILEKQLDLISNRSVFWTNYGLRSLSKTSSLYMKYNTEHDAPYWRGTIWMNMNYLILSALN 780

Query: 781 HYATEHGPYREKAKDIRDELRSNIIRNVVRNYQKTGYIWEQYNQKTGKGKGAHPFTGWTS 840
           HYATE GPYRE+AKDIRDELRSN+IRNVV NY KTG++WEQYNQKTGKGKGAHPFTGWTS
Sbjct: 781 HYATEDGPYRERAKDIRDELRSNLIRNVVGNYHKTGFVWEQYNQKTGKGKGAHPFTGWTS 840

Query: 841 LLLLIMAEAYTES 853
           L+LLIMAEAYTES
Sbjct: 841 LVLLIMAEAYTES 853

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HTM30.0e+0064.21Mannosyl-oligosaccharide glucosidase GCS1 OS=Arabidopsis thaliana OX=3702 GN=GCS... [more]
Q84M892.9e-30362.24Alpha-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GCS2 PE=2 SV=1[more]
Q80UM78.6e-15439.25Mannosyl-oligosaccharide glucosidase OS=Mus musculus OX=10090 GN=Mogs PE=1 SV=1[more]
O889412.3e-15138.74Mannosyl-oligosaccharide glucosidase OS=Rattus norvegicus OX=10116 GN=Mogs PE=1 ... [more]
Q137248.9e-15138.83Mannosyl-oligosaccharide glucosidase OS=Homo sapiens OX=9606 GN=MOGS PE=1 SV=5[more]
Match NameE-valueIdentityDescription
XP_004135311.10.0100.00mannosyl-oligosaccharide glucosidase GCS1 [Cucumis sativus] >KGN51681.1 hypothet... [more]
XP_008446049.10.094.96PREDICTED: mannosyl-oligosaccharide glucosidase GCS1-like [Cucumis melo] >KAA003... [more]
TYK15717.10.092.57mannosyl-oligosaccharide glucosidase GCS1-like [Cucumis melo var. makuwa][more]
XP_038892087.10.090.97mannosyl-oligosaccharide glucosidase GCS1 isoform X4 [Benincasa hispida][more]
XP_038892086.10.090.55mannosyl-oligosaccharide glucosidase GCS1 isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KVA60.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589980 PE=3 SV=1[more]
A0A5A7SUQ80.094.96Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BE510.094.96mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5D3CUY70.092.57Mannosyl-oligosaccharide glucosidase GCS1-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1H1180.088.75mannosyl-oligosaccharide glucosidase GCS1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031631Glycosyl hydrolase family 63, N-terminalPFAMPF16923Glyco_hydro_63Ncoord: 108..273
e-value: 2.5E-42
score: 145.5
IPR031335Glycosyl hydrolase family 63, C-terminalPFAMPF03200Glyco_hydro_63coord: 350..565
e-value: 2.0E-85
score: 287.4
IPR038518Glycosyl hydrolase family 63, N-terminal domain superfamilyGENE3D2.70.98.110coord: 96..378
e-value: 3.6E-74
score: 252.2
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 379..562
e-value: 1.1E-65
score: 224.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..35
NoneNo IPR availablePANTHERPTHR10412:SF17ALPHA-GLUCOSIDASE 2coord: 79..560
IPR004888Glycoside hydrolase family 63PANTHERPTHR10412MANNOSYL-OLIGOSACCHARIDE GLUCOSIDASEcoord: 79..560
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 363..557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G9991.T4Cucsat.G9991.T4mRNA
Cucsat.G9991.T1Cucsat.G9991.T1mRNA
Cucsat.G9991.T2Cucsat.G9991.T2mRNA
Cucsat.G9991.T5Cucsat.G9991.T5mRNA
Cucsat.G9991.T11Cucsat.G9991.T11mRNA
Cucsat.G9991.T7Cucsat.G9991.T7mRNA
Cucsat.G9991.T6Cucsat.G9991.T6mRNA
Cucsat.G9991.T3Cucsat.G9991.T3mRNA
Cucsat.G9991.T9Cucsat.G9991.T9mRNA
Cucsat.G9991.T8Cucsat.G9991.T8mRNA
Cucsat.G9991.T10Cucsat.G9991.T10mRNA
Cucsat.G9991.T13Cucsat.G9991.T13mRNA
Cucsat.G9991.T12Cucsat.G9991.T12mRNA
Cucsat.G9991.T14Cucsat.G9991.T14mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009311 oligosaccharide metabolic process
biological_process GO:0006487 protein N-linked glycosylation
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004573 Glc3Man9GlcNAc2 oligosaccharide glucosidase activity