Cucsat.G9673 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G9673
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome b561 domain-containing protein
Location	ctg1673: 4912915 .. 4914861 (-)
RNA-Seq Expression	Cucsat.G9673
Synteny	Cucsat.G9673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTTCACTAATGTTTTAAATTTGATATGCTTTGCCAAAGATCCAAGTTTTCCCATCACTACCAATTCCAAACCCAGCTCTTCAAGCAACATTTTATATCCCTCTCTCCAAATACAAATACTATATATAGGAACCAACATGAAACTCAAAGTCTTTGAGAACAAAAAATATACATTATTAGGTAGTCCCCATTGAATATGGACCCTATGGAGAGGGCAAATTTTAGGCACACTGCTTCTACAATTACTGTCTTTGCTCATATTTTTGGTCTCACTGCCTGCATTCTTACCCTTGTTTGGTTGCTGCATTATCGTGGCGGCCTTGCCTACGATTCTCCCAATTCCAACCTTGTTTTCAATGTAAGGATTCACTCGTTTCTTTCGTCATTTCACTTTACTTGTGGCTTTGATGTTATTGGTGTTTGATCTAGGAATATCAACTTGAATTTATTTTTGACGGAAAGAACTAAAATTATGTTTTAATACCACTTTGGCCTCTATCCATTTGAAATAAAAATAAAGGGACCAAAATGATTCTTATAAATGAACTAAATTAAAAAATGCAAGGTATTTACACTTATATTATACTTATATTTCTCTTAACTCTATCTTGTTTCAATAATTTCTTAGTTTTATCTTCTACCAACTTCCATATATTATCAAGTTTTTGAAATAATGTTCAATAGAAGAGAAAAAAAATGATATTGAGTTACTAATTCCTCCTAATGAATTTCATAGTTCAATTAGATTGTTATCACTAAGGGTCAATTTGGCTAATATGACCAAAACATAGAAGTGTTACCATGAACAGAAGAGAAACTAACATCACAGATTTTTTTTTTTTTTTTGACAGTGTCACCCATTCTTCATGCTCCTCGGTTTCGTGTTCATGTCGGGGGAAGGTACCACAACTAGCTCGGGTTTATTTAACCAATATTTTGAAGATACAATATATATATATATAATAACCAAAAATAACCATGATTGAATTGGGTTTGAGCAGCAATGATGGCCTACAGGACAGTGAGGGCAGAAAAGAGAACAAAGAAGATAGTTCACATGGCTCTTCATTTGGTAGCTATTTGCATGGGAATTATAGGGATAAATGCTGCTTTCAAATTTCATGATCAAGTGAACTTGGAGGATATGTATAGTTTACATTCATGGATTGGTATAGTCACTTTTTGCCTTTACGGTTTACAGGTATATTAAGTTTTAATTAGACCCTTAAACTTTCAAAACTTCCATCATCTTTTAAGTAAGTAGACTAACTTACATTGGTTTGGACAGTGGGTGGTGGGACTATTTACATTCTTGTTTCCAGGAGCAGATGGAGAGACTAAAACTAGGTTGCTACCATGGCACATAACTGGGGGAAGAGCTCTACTATTGATGATAATATGCACTGCGTTGACTGGCTTGTCTGAGAAGGCTACTTCTTTGAGACTGACTAGCAGCCACGAGTCCCGTTTGCTTAACTTCACTGCTTTCTTCGTCCTCCTCTTTGGCATTTCCATCGATATCTCCATCGGTTTTGCTCGATTTATGCACGTTGATCACACCATTTAGTTCTTCTTTTCCCTCTCTTTTCCTAATATTCATGACCTAATCTTTTCTGATTTCTTGTTTTTTCGAGTGTGACCTTGTTTACAAGTTGTTTCTTTCTTCGTCCTCCTCTTTGGCATTTCCATCGATATCTCCATCGGTTTTGCTCGATTTATGCACGTTGATCACACCATTTAGTTCTTCTTTTCCCTCTCTTTTCCTAATATTCATGACCTAATCTTTTCTGATTTCTTGTTTTTACGAGTGTGACCTTGTTTACAAGTTGTTTTTAAGTCTCATTGTTGAGATGTGTGTTGACTGTTAAGATTATGTTTATTCCAACTCAATGGACCTCTTGTCCTCTTATTTTGAATGAAGACAAAATTTGTTAGGTTTGAAACGG

Coding sequence (CDS)

ATGGACCCTATGGAGAGGGCAAATTTTAGGCACACTGCTTCTACAATTACTGTCTTTGCTCATATTTTTGGTCTCACTGCCTGCATTCTTACCCTTGTTTGGTTGCTGCATTATCGTGGCGGCCTTGCCTACGATTCTCCCAATTCCAACCTTGTTTTCAATTGTCACCCATTCTTCATGCTCCTCGGTTTCGTGTTCATGTCGGGGGAAGCAATGATGGCCTACAGGACAGTGAGGGCAGAAAAGAGAACAAAGAAGATAGTTCACATGGCTCTTCATTTGGTAGCTATTTGCATGGGAATTATAGGGATAAATGCTGCTTTCAAATTTCATGATCAAGTGAACTTGGAGGATATGTATAGTTTACATTCATGGATTGGTATAGTCACTTTTTGCCTTTACGGTTTACAGTGGGTGGTGGGACTATTTACATTCTTGTTTCCAGGAGCAGATGGAGAGACTAAAACTAGGTTGCTACCATGGCACATAACTGGGGGAAGAGCTCTACTATTGATGATAATATGCACTGCGTTGACTGGCTTGTCTGAGAAGGCTACTTCTTTGAGACTGACTAGCAGCCACGAGTCCCGTTTGCTTAACTTCACTGCTTTCTTCGTCCTCCTCTTTGGCATTTCCATCGATATCTCCATCGGTTTTGCTCGATTTATGCACGTTGATCACACCATTTAG

Protein sequence

MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI

Homology

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BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot
Match: Q67ZF6 (Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=3702 GN=CYB561C PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.7e-62
Identity = 112/218 (51.38%), Postives = 153/218 (70.18%), Query Frame = 0


Query: 5   ERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGF 64
           +R   +  +S  T+ AH FG+ A +L L+WLLHYR G+ Y S N   V N HPF M  GF
Sbjct: 6   DRTTLKRHSSLSTLVAHFFGILAVVLMLIWLLHYREGIEYGSDNPLKVLNVHPFLMYCGF 65

Query: 65  VFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHS 124
           +F+ G+AMM Y+T  A  + +K+VH  LHL+ + +GI+GI AAF+FHD+VNL+DM SLHS
Sbjct: 66  LFLVGQAMMTYKTAYASHQVQKMVHGGLHLIGLVLGIVGICAAFRFHDKVNLKDMVSLHS 125

Query: 125 WIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEK 184
           WIG+ TF L G+QW+ G FTFL P +   T+TR++PWH+ GGRALL M I  ALTGL ++
Sbjct: 126 WIGLTTFILLGVQWLFGAFTFLAPQSSSGTRTRMMPWHVLGGRALLYMGIVAALTGLMQR 185

Query: 185 ATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARF 223
           AT L  +++ ESRL+NF    +LLFG+S+D S+   R+
Sbjct: 186 ATMLGQSTNAESRLINFLGLAILLFGVSVDFSVALGRY 223

[Close]

BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 6.1e-46
Identity = 87/209 (41.63%), Postives = 131/209 (62.68%), Query Frame = 0


Query: 13  ASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGFVFMSGEAM 72
           A+  T  AH   + A  + LVW +H+RGGLA+++ N NL+FN HP  ML+G++ +  EA+
Sbjct: 7   AAPFTYVAHALAVAAATMVLVWCIHFRGGLAFEATNKNLIFNVHPVLMLIGYIILGSEAI 66

Query: 73  MAYRTVRAEKR-TKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHSWIGIVTF 132
           M Y+ +   K  T K++H+ LH +A+  G +GI  AFKFH++  + ++YSLHSW+GI T 
Sbjct: 67  MVYKVLPTWKHDTTKLIHLILHAIALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTI 126

Query: 133 CLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEKATSLRLT 192
           CLYG+QW+ G   F FP A    +  +LPWHI  G  + ++ + TA  G  EK T L+ +
Sbjct: 127 CLYGIQWIFGFVAFFFPRASPSVRKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSS 186

Query: 193 S----SHESRLLNFTAFFVLLFGISIDIS 217
                  E+ L+NFTA  V+LFG S+ ++
Sbjct: 187 GLDKYGAEAFLVNFTALIVVLFGASVVVA 215

[Close]

BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.3e-45
Identity = 89/205 (43.41%), Postives = 126/205 (61.46%), Query Frame = 0


Query: 10  RHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGFVFMSG 69
           R  A  +T  AH   + A I+ LVW + YRGGLA+++ N NL+FN HP  ML+GF+ + G
Sbjct: 4   RINAMAVTFVAHALAVIAAIMVLVWSISYRGGLAWEATNKNLIFNLHPVLMLIGFIILGG 63

Query: 70  EAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHSWIGIV 129
           EA+++Y+++  EK  KK++H+ LH +A+ +GI GI AAFK H++ ++ ++YSLHSWIGI 
Sbjct: 64  EAIISYKSLPLEKPVKKLIHLILHAIALALGIFGICAAFKNHNESHIPNLYSLHSWIGIG 123

Query: 130 TFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEKATSLR 189
              LYG QWV     F FPG     K+ LLPWH   G  + ++ +  A  G  EK T L 
Sbjct: 124 VISLYGFQWVYSFIVFFFPGGSTNLKSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLE 183

Query: 190 LTS----SHESRLLNFTAFFVLLFG 211
                    E+ L+NFTA   +LFG
Sbjct: 184 NGGLDKYGSEAFLINFTAIITILFG 208

[Close]

BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 8.7e-45
Identity = 89/209 (42.58%), Postives = 131/209 (62.68%), Query Frame = 0


Query: 13  ASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGFVFMSGEAM 72
           A+  T  AH   + A  + LVW +++RGGLA ++ N NL+FN HP  ML+G++ + GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWAIYFRGGLAIEATNKNLIFNVHPVLMLIGYIIIGGEAI 68

Query: 73  MAYRTV-RAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHSWIGIVTF 132
           M YR +  +   T K++H+ LH +A+ +G +GI  AFK H++  + ++YSLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHETNKLIHLVLHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 133 CLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEKATSLRLT 192
            LYG+QW+VG  TF FPGA    K  +LPWHI  G  + ++ +  A  G  EK T L  +
Sbjct: 129 TLYGIQWIVGFVTFFFPGAAPNVKKGVLPWHILFGLFVYILALANAELGFLEKLTFLESS 188

Query: 193 S----SHESRLLNFTAFFVLLFGISIDIS 217
                  E+ L+NFTA  V+LFG S+ ++
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASVVVA 217

[Close]

BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-44
Identity = 89/209 (42.58%), Postives = 129/209 (61.72%), Query Frame = 0


Query: 13  ASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFMLLGFVFMSGEAM 72
           A+  T  AH   + A  + LVW + +RGGLA +S N NL+FN HP  ML+G+V + GEA+
Sbjct: 9   AAPFTYAAHALAVAAAAMVLVWSIQFRGGLAIESTNKNLIFNVHPVLMLIGYVIIGGEAI 68

Query: 73  MAYRTVRAEKR-TKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMYSLHSWIGIVTF 132
           M YR +      T K++H+ LH +A+ +G +GI  AFK H++  + ++YSLHSWIGI T 
Sbjct: 69  MVYRVLPTSNHDTTKLIHLILHGIALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTI 128

Query: 133 CLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTGLSEKATSLRLT 192
            LYG+QW++G  TF FPGA    K  +LPWH+  G  + ++ +  A  G  EK T L  +
Sbjct: 129 TLYGIQWIIGFVTFFFPGAAPNVKKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESS 188

Query: 193 S----SHESRLLNFTAFFVLLFGISIDIS 217
                  E+ L+NFTA  V+LFG S+ ++
Sbjct: 189 GLDKYGTEAFLVNFTALVVVLFGASVVVA 217

[Close]

BLAST of Cucsat.G9673 vs. NCBI nr
Match: XP_004142817.1 (probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >KGN51448.1 hypothetical protein Csa_009397 [Cucumis sativus])

HSP 1 Score: 461 bits (1185), Expect = 1.38e-163
Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWVVGLFTFLFPGAD ETKTRLLPWHITGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229
           LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI
Sbjct: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229

[Close]

BLAST of Cucsat.G9673 vs. NCBI nr
Match: XP_008466953.1 (PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo])

HSP 1 Score: 434 bits (1115), Expect = 6.49e-153
Identity = 213/229 (93.01%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MDPMERANFRHTASTI VFAHI GLTACIL LVWLLHYRGGLAYDSP++NLVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVFMSGEAMMAYRTVRAEK TKKIVHM LHLVAICMGIIGINAAFKFHDQVNLEDMY
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GLFTFLFPGAD  TKTRLLPWHI+GGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229
           LSEKATSLRLT SHESRL NFTAFFVLLFGISIDISI FARFMHVDHTI
Sbjct: 181 LSEKATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHTI 229

[Close]

BLAST of Cucsat.G9673 vs. NCBI nr
Match: XP_038907208.1 (probable transmembrane ascorbate ferrireductase 3 [Benincasa hispida])

HSP 1 Score: 424 bits (1090), Expect = 4.20e-149
Identity = 206/229 (89.96%), Postives = 218/229 (95.20%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD M+RA FRHTAS+ITVFAHIFGLTACIL LVWLLHYRGGLAYDSPN +LVFNCHPFFM
Sbjct: 1   MDTMDRATFRHTASSITVFAHIFGLTACILMLVWLLHYRGGLAYDSPNPDLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVFMSGEAMMAY+TVRAEK TKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDM+
Sbjct: 61  LLGFVFMSGEAMMAYKTVRAEKITKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GLFTFLFPGAD  TK RLLPWHI+GGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAATKARLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229
           LSEKATSLRL  S ESRLLNFTAFFVLLFGISID+SIGFARFMH+DHT+
Sbjct: 181 LSEKATSLRLAGSPESRLLNFTAFFVLLFGISIDVSIGFARFMHIDHTV 229

[Close]

BLAST of Cucsat.G9673 vs. NCBI nr
Match: XP_022991076.1 (probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima])

HSP 1 Score: 379 bits (974), Expect = 2.00e-131
Identity = 184/228 (80.70%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD ++R  FRHTAS I V AHIFGLTACILTLVWLLHYRGGLAYDS ++ LVFNCHPFFM
Sbjct: 1   MDTVDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVF+SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI GINA+FKFHD + LE ++
Sbjct: 61  LLGFVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228
           LS+KATS  LT +HESRLLNFT FFVLLFGISIDI+IGF RF H D T
Sbjct: 181 LSQKATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLT 228

[Close]

BLAST of Cucsat.G9673 vs. NCBI nr
Match: KAG6601895.1 (putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 378 bits (971), Expect = 5.72e-131
Identity = 183/228 (80.26%), Postives = 201/228 (88.16%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD + R  FRHTAS I   AHIFGLTACILTLVWLLHYRGGLAYDS +++LVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFV +SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228
           LS+KATS  LT +HESRLL FT FFVLLFGISIDI+IGF RF HVD T
Sbjct: 181 LSQKATSSTLTGNHESRLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 228

[Close]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL
Match: A0A0A0KPB5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G548140 PE=4 SV=1)

HSP 1 Score: 461 bits (1185), Expect = 6.67e-164
Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQWVVGLFTFLFPGAD ETKTRLLPWHITGGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADRETKTRLLPWHITGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229
           LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI
Sbjct: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229

[Close]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL
Match: A0A1S3CSD9 (probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC103504338 PE=4 SV=1)

HSP 1 Score: 434 bits (1115), Expect = 3.14e-153
Identity = 213/229 (93.01%), Postives = 219/229 (95.63%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MDPMERANFRHTASTI VFAHI GLTACIL LVWLLHYRGGLAYDSP++NLVFNCHPFFM
Sbjct: 1   MDPMERANFRHTASTIAVFAHISGLTACILMLVWLLHYRGGLAYDSPDANLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVFMSGEAMMAYRTVRAEK TKKIVHM LHLVAICMGIIGINAAFKFHDQVNLEDMY
Sbjct: 61  LLGFVFMSGEAMMAYRTVRAEKITKKIVHMGLHLVAICMGIIGINAAFKFHDQVNLEDMY 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GLFTFLFPGAD  TKTRLLPWHI+GGRALL+MIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLFTFLFPGADAMTKTRLLPWHISGGRALLMMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHTI 229
           LSEKATSLRLT SHESRL NFTAFFVLLFGISIDISI FARFMHVDHTI
Sbjct: 181 LSEKATSLRLTGSHESRLFNFTAFFVLLFGISIDISISFARFMHVDHTI 229

[Close]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL
Match: A0A6J1JPQ6 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN=LOC111487782 PE=4 SV=1)

HSP 1 Score: 379 bits (974), Expect = 9.68e-132
Identity = 184/228 (80.70%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD ++R  FRHTAS I V AHIFGLTACILTLVWLLHYRGGLAYDS ++ LVFNCHPFFM
Sbjct: 1   MDTVDRPGFRHTASAIAVLAHIFGLTACILTLVWLLHYRGGLAYDSRSAELVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVF+SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI GINA+FKFHD + LE ++
Sbjct: 61  LLGFVFVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIAGINASFKFHDMIKLEHVF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFCLYGLQWLLGLCTFLFPGASRETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228
           LS+KATS  LT +HESRLLNFT FFVLLFGISIDI+IGF RF H D T
Sbjct: 181 LSQKATSSTLTGNHESRLLNFTGFFVLLFGISIDIAIGFDRFRHADLT 228

[Close]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL
Match: A0A6J1CYG4 (probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673 GN=LOC111015617 PE=4 SV=1)

HSP 1 Score: 377 bits (969), Expect = 5.21e-131
Identity = 181/220 (82.27%), Postives = 201/220 (91.36%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD MERA+FRHTAS +T+ AHIFG+TACIL LVWLLHYRGGL YDSPN NLVFNCHPFFM
Sbjct: 1   MDTMERASFRHTASAVTIMAHIFGVTACILLLVWLLHYRGGLGYDSPNPNLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFVF+SGEAMMA++TVRA+K  +KI HM LHLVAICMGI GINAAFKFHD VNLEDM+
Sbjct: 61  LLGFVFVSGEAMMAFKTVRADKMARKITHMGLHLVAICMGIAGINAAFKFHDLVNLEDMF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           SLHSWIGIVTF LYGLQWV+GL TFLFPGA+  TK +LLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SLHSWIGIVTFSLYGLQWVLGLCTFLFPGAEASTKAKLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFA 220
           LS+KATSL+L ++HESRLLNFTAFFVLLFGISI+I +GFA
Sbjct: 181 LSQKATSLKLVANHESRLLNFTAFFVLLFGISINIVVGFA 220

[Close]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL
Match: A0A6J1HF52 (probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 GN=LOC111462470 PE=4 SV=1)

HSP 1 Score: 376 bits (966), Expect = 1.60e-130
Identity = 181/228 (79.39%), Postives = 201/228 (88.16%), Query Frame = 0


Query: 1   MDPMERANFRHTASTITVFAHIFGLTACILTLVWLLHYRGGLAYDSPNSNLVFNCHPFFM 60
           MD + R  FRHTAS I   AHIFGLTACILTLVWLLHYRGGLAYDS +++LVFNCHPFFM
Sbjct: 1   MDTVNRPGFRHTASAIAALAHIFGLTACILTLVWLLHYRGGLAYDSRSADLVFNCHPFFM 60

Query: 61  LLGFVFMSGEAMMAYRTVRAEKRTKKIVHMALHLVAICMGIIGINAAFKFHDQVNLEDMY 120
           LLGFV +SGEAMMAY+TVRA+K T+KIVHMALH VAICMGI+GINA+FKFHD + LE M+
Sbjct: 61  LLGFVIVSGEAMMAYKTVRADKMTRKIVHMALHFVAICMGIVGINASFKFHDMIKLEHMF 120

Query: 121 SLHSWIGIVTFCLYGLQWVVGLFTFLFPGADGETKTRLLPWHITGGRALLLMIICTALTG 180
           S+HSWIGIVTFCLYGLQW++GL TFLFPGA  ETK RLLPWHI+GGRALLLMIICTALTG
Sbjct: 121 SIHSWIGIVTFCLYGLQWLLGLCTFLFPGAGSETKARLLPWHISGGRALLLMIICTALTG 180

Query: 181 LSEKATSLRLTSSHESRLLNFTAFFVLLFGISIDISIGFARFMHVDHT 228
           LS+KATS  LT +HE+RLL FT FFVLLFGISIDI+IGF RF HVD T
Sbjct: 181 LSQKATSSTLTGNHEARLLKFTGFFVLLFGISIDIAIGFDRFRHVDPT 228

The following BLAST results are available for this feature:

BLAST of Cucsat.G9673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67ZF6	2.7e-62	51.38	Probable transmembrane ascorbate ferrireductase 3 OS=Arabidopsis thaliana OX=370...	[more]
A3A9H6	6.1e-46	41.63	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]
Q8L856	2.3e-45	43.41	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
C4IYS8	8.7e-45	42.58	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
Q6I681	2.5e-44	42.58	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]

BLAST of Cucsat.G9673 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142817.1	1.38e-163	99.56	probable transmembrane ascorbate ferrireductase 3 [Cucumis sativus] >KGN51448.1 ...	[more]
XP_008466953.1	6.49e-153	93.01	PREDICTED: probable transmembrane ascorbate ferrireductase 3 [Cucumis melo]	[more]
XP_038907208.1	4.20e-149	89.96	probable transmembrane ascorbate ferrireductase 3 [Benincasa hispida]	[more]
XP_022991076.1	2.00e-131	80.70	probable transmembrane ascorbate ferrireductase 3 [Cucurbita maxima]	[more]
KAG6601895.1	5.72e-131	80.26	putative transmembrane ascorbate ferrireductase 3, partial [Cucurbita argyrosper...	[more]

BLAST of Cucsat.G9673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPB5	6.67e-164	99.56	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G54...	[more]
A0A1S3CSD9	3.14e-153	93.01	probable transmembrane ascorbate ferrireductase 3 OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A6J1JPQ6	9.68e-132	80.70	probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1CYG4	5.21e-131	82.27	probable transmembrane ascorbate ferrireductase 3 OS=Momordica charantia OX=3673...	[more]
A0A6J1HF52	1.60e-130	79.39	probable transmembrane ascorbate ferrireductase 3 OS=Cucurbita moschata OX=3662 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 1..110 e-value: 2.4E-34 score: 130.1
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 1..115 e-value: 2.0E-33 score: 115.5
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 1..147 score: 33.867134
None	No IPR available	GENE3D	1.20.120.1770		coord: 1..148 e-value: 5.7E-48 score: 165.0
None	No IPR available	PANTHER	PTHR10106:SF15	TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 3-RELATED	coord: 1..149
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 1..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G9673.T1	Cucsat.G9673.T1	mRNA
Cucsat.G9673.T2	Cucsat.G9673.T2	mRNA
Cucsat.G9673.T3	Cucsat.G9673.T3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity