Cucsat.G9472 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G9472
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Cytochrome P450
Location	ctg1673: 1102077 .. 1105436 (-)
RNA-Seq Expression	Cucsat.G9472
Synteny	Cucsat.G9472

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTTTCTCACTACTTGCCCCCAACAGGGACTTCAAGTCATTCTTCGTTCGATATCAAATTAGAATAAAGAAAAAAACAAAAACGTAAATTTGTTTGATGTAAGAATAGATTGTTTTAACTTTAACACCGAAAAGGTATTGTAAGTTATTGAGCTCTGCATTATTGAGCTTTGAAGTATGAATGAAGGCAGGCGAATACGTTGACTTCCTCTGATTTTTCCCCTTTATCTTCATCCTCCCACTCCCTCCCTCCATCCCTTCGTTCCTGCTATTTTAACCTCTGGCGCCGTAATCATTTTCCTCCGCTCTCTAGTTTAATTGGGACCAAACCTCAGGTTTGTGTTTTTCTCTTTCAAACTTTTTGTTAATCAAAAAAGAGAAAAAGTACTTCAGAGTATCAAACTGAATCAAAGCAACCTAACTTTCAATTTTCAATCTTAATAATTACAAATGGAAGTTTCTCAGAAACCGATTGTCTTTCAATTGCAATATCAGATGCCATCTTGAAGCAATTAGAAGAAAAAGTGAGATGTGATGGAGTGTACGATCATCAATTACATTTCAATATTTCTATCTGGGTGTCTTCTTTTTGGGGTCTTCAAGCTCTTTGTCAAACTATGGTGGACTCCCATGAGGATTCAGAGGTTCATGAGGTCACAGGGGATTCAAGGTCCTTCTTTTAAGTTCATCCAAGGCAATACCAGAGATGTTTACATCAAAAGAATGCAGGCCATGGCTACACCCATGGACCTTTCCCATAACATTCTCCACAGAGTTATGCCTTGTGTTCACTCTTGGCTCAACCTTTACGGTACAAAAATCATCTTGAACTTTCAATTGTTGTGTTGGGACAGCCTTGAATCCGTTATCTGAAACTTTGAATGTAAATGACCAACCAAATGAGATTTATGTTATTTACGACTGTGGATTCAAGGCTTAGAGATGTGTCATATATAAACCTTTTAATCTTACTTGCCATTTTTGCAGTCTATATTATTTGATAGTTTAACACACTGATAGTGAAGTATGTAAATCTGTGTTGATCTTTTCTCACTTTCTTTGCTTGGTTTGTCAATTTAATAACATTTTCCTTTTCGTTAACGACTGAGTCTGGTTTCTAAACTTTTAGAGCTGTGCTTTTAGGAATAGATTCTTGAATTTTAAATAGTATTGTAATAGGTCTCTTAACCATTGCTTTTGAGTATAATATCTTGCAAACGAATGGTTATTACTTATTAAATACGAAATTGAGACTTAGACTTGATTGTTGTCTAAATCTATTAAAAAATGTTAAAGGGGTCTATTAAACTCAACTTTGAAAGTTAATGAATTAACTTATAATTAAACAGCTTCTTTTTCAATTGGATGTCTGTTTTTATCGGTGCGAGTGCTTGGAATTGTCAAACAGGGAGGAACTATCTTCAGTGGTCTGGAGTTGATGCTCAGTTAATGATTACAGACCCAGAGATGATCAAGGAGGTGCTCCATGATCGACAAAAGAGCTTCCCAAAAGCAAAACTCAAAGGCCACATTCATAGAATTTTTGGAAATGGACTTGCCACAGCAGAGGGTCAAAGATGGGCCAACTCCAGGAGAATAGCTAACTTTGCTTTCCATGGAGACAGTCTCAAAGTAATTAACTTCATACTTGATTTTAGTTTTGTCGAGATTTGATTGAACTGTTTGAGATTATTGCGAGTGTACTGCAGAACATGATCCCAACCATGATTGAGTGTGGTGAGAAGATGATTGAAGGGTGGAAGAATTATGAAGGCAAAGAATTGGATGCGTTCAAAGAATTCAAAGTATTCACTTTGGATGTCATTTCTCATACTGCTTTTGGGAGTAGTTACCAACAAGGAAAGAAAATCTTCCACATGCTACGAGAGCTTTGCGAGCTATCAATCAGAAATGGATACAAAATTAGGCTACCTATAATCAGGTAATTTAGCCTTGTTCAATAGTTGAAAATTGAACTTATAAACATTACTCTCAAGACTTGGAATTCAAATGTTTTAGTTCTATCAAATGGGGGGCTTACTTTTGTTTGTTGAGTTCCTAAATTACTTGATGAAAGTTGATGTTATGTTAATAGAAATGAAACTATTTGTTGGAATATTTTGATTTTGTTATCTGGTACTCCAAATGATCAAGAACGGGGTCTCGCTAATTATAAAACTACTTTCACTTTGCTCGTGGACATAGCCAGCATACTGTTAGTAAAACATTTTTTGTGTCGTTTTTTTTACTGTTGATGCTTTTTGCTTGTGTCTTAACTCTAGTTTTGATTTGTTTGCAGTAAAATTTTGAAATCAAAAGATGATTATGAAGGAGAAAGGCTTGGAAAGAGACTTAAAGATTGCTTCATGGAAATCATAAAAGAGAGAGAAGAGAAGTTGAGGAATGGTGAAGCAAATGATTATGGGAATGACTTTCTGGGATTATTAATAAAGGCGAAGAACGACCCTGAAACATCACAAAGCATTTCTATGGAAGACATTGTTGATGAATGCAAAACATTTTACTTTGCTGGACATGAGACCACCAATGTTTTGTTGGCTTGGACTATGCTTCTTTTGGCTTTGCATAAAGAATGGCAAGAAAAAGCAAGAAATGAAGTGTTGGATGTATTTGGACACAACAACCCAACTTTGGAAGGCCTTCCAAAACTAAAAACAGTAAGAATTATGACGTATAGATGAATATGGAAACTAGGATCTCCTGATCTATTATCATGTAAATACTTATACAAACTGTGTGTTTGCTTTTACTATGGACAGATGGCGATGATCATCAATGAATGTCTGAGGCTATATCCACCGGCAATGCCAGTAGCACGGCGAGTAGAGAAGGAAGTGAGACTAGGAAACCTGGTGGTTCCTACAGCGACTATGCTGACAATTCCTACAGTAGCAGTTCATCATGACACAACGTTTTGGGGGGAGGACGCACATGAATTCAAACCAGAAAGATTTTCAGAAGGGGTGGGGAAAGCCACCGAAAACAACTCGGCTGCGTATATCCCGTTCGGATTGGGGCCTCGAAACTGTGTGGGGATGAACTTTGCAATGAACGAAGCCAAAATTGCAATGTCAATGATTCTACAGAGATACTCCTTCAGGCTATCGCCGGCCTATGCACACATGCCGGCGCAGTTGTTAACCATTAGCCCCCAGAATGGAGTTCAAGTGATACTAAATTCAATAGCAGATTAAAAAGATTGAAATGAACACTGTTTGGGTGTTAATAAGATACATGTATGTATGGTTGGTACTCTAAATTTGAACAAATATATGGCAAAACTACATTATTATTATTATTATTATTATTACTACGATTGGTTCGGTCTTTCTCCCTTGC

Coding sequence (CDS)

ATGGAGTGTACGATCATCAATTACATTTCAATATTTCTATCTGGGTGTCTTCTTTTTGGGGTCTTCAAGCTCTTTGTCAAACTATGGTGGACTCCCATGAGGATTCAGAGGTTCATGAGGTCACAGGGGATTCAAGGTCCTTCTTTTAAGTTCATCCAAGGCAATACCAGAGATGTTTACATCAAAAGAATGCAGGCCATGGCTACACCCATGGACCTTTCCCATAACATTCTCCACAGAGTTATGCCTTGTGTTCACTCTTGGCTCAACCTTTACGGGAGGAACTATCTTCAGTGGTCTGGAGTTGATGCTCAGTTAATGATTACAGACCCAGAGATGATCAAGGAGGTGCTCCATGATCGACAAAAGAGCTTCCCAAAAGCAAAACTCAAAGGCCACATTCATAGAATTTTTGGAAATGGACTTGCCACAGCAGAGGGTCAAAGATGGGCCAACTCCAGGAGAATAGCTAACTTTGCTTTCCATGGAGACAGTCTCAAAAACATGATCCCAACCATGATTGAGTGTGGTGAGAAGATGATTGAAGGGTGGAAGAATTATGAAGGCAAAGAATTGGATGCGTTCAAAGAATTCAAAGTATTCACTTTGGATGTCATTTCTCATACTGCTTTTGGGAGTAGTTACCAACAAGGAAAGAAAATCTTCCACATGCTACGAGAGCTTTGCGAGCTATCAATCAGAAATGGATACAAAATTAGGCTACCTATAATCAGTAAAATTTTGAAATCAAAAGATGATTATGAAGGAGAAAGGCTTGGAAAGAGACTTAAAGATTGCTTCATGGAAATCATAAAAGAGAGAGAAGAGAAGTTGAGGAATGGTGAAGCAAATGATTATGGGAATGACTTTCTGGGATTATTAATAAAGGCGAAGAACGACCCTGAAACATCACAAAGCATTTCTATGGAAGACATTGTTGATGAATGCAAAACATTTTACTTTGCTGGACATGAGACCACCAATGTTTTGTTGGCTTGGACTATGCTTCTTTTGGCTTTGCATAAAGAATGGCAAGAAAAAGCAAGAAATGAAGTGTTGGATGTATTTGGACACAACAACCCAACTTTGGAAGGCCTTCCAAAACTAAAAACAATGGCGATGATCATCAATGAATGTCTGAGGCTATATCCACCGGCAATGCCAGTAGCACGGCGAGTAGAGAAGGAAGTGAGACTAGGAAACCTGGTGGTTCCTACAGCGACTATGCTGACAATTCCTACAGTAGCAGTTCATCATGACACAACGTTTTGGGGGGAGGACGCACATGAATTCAAACCAGAAAGATTTTCAGAAGGGGTGGGGAAAGCCACCGAAAACAACTCGGCTGCGTATATCCCGTTCGGATTGGGGCCTCGAAACTGTGTGGGGATGAACTTTGCAATGAACGAAGCCAAAATTGCAATGTCAATGATTCTACAGAGATACTCCTTCAGGCTATCGCCGGCCTATGCACACATGCCGGCGCAGTTGTTAACCATTAGCCCCCAGAATGGAGTTCAAGTGATACTAAATTCAATAGCAGATTAA

Protein sequence

MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD

Homology

[Close]

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot
Match: H2DH17 (Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 6.8e-154
Identity = 261/517 (50.48%), Postives = 362/517 (70.02%), Query Frame = 0


Query: 1   MECTIINYISIFLSGC--LLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRD 60
           +E T I +  +  S C  LLF   +    +WWTP+R+QR  R QGI+GPS+ F+ GNT++
Sbjct: 9   VEMTPILFQFLLSSLCVFLLFVFIRFLNDIWWTPIRLQRVFRQQGIRGPSYGFLYGNTKE 68

Query: 61  VYIKRMQAMATPMD-LSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEV 120
           +   R ++M+ PMD LSHNI  R+ P ++SWLN+YG+N+L W G  AQ ++T  + +KE 
Sbjct: 69  ILNMRKESMSRPMDYLSHNIFPRLQPHLYSWLNIYGKNFLNWYGPRAQFVVTQVDFVKET 128

Query: 121 LHDRQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECG 180
           +  + +++PK   +    ++ G+G+ T++G++WA  RR+AN AFH +SLK+M P MI   
Sbjct: 129 M-IKDQAYPKMDPEWFAKKLLGDGIVTSKGKKWAKHRRLANHAFHAESLKSMTPAMIASV 188

Query: 181 EKMIEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGY 240
           E M++ WK +EG+E+D F+EFK+ T +VIS TAFGSSY  GK IF  L +L  +  RN Y
Sbjct: 189 EMMLKRWKQHEGREIDVFQEFKILTSEVISRTAFGSSYLDGKDIFDRLTQLGIIITRNSY 248

Query: 241 KIRLPIISKILKSKDDYEGERLGKRLKDCFMEIIKERE-EKLRNGEANDYGNDFLGLLIK 300
           K++LP IS   KS D+ E E+L + L D  + I+++RE E   +GE   +G DFLGLL+K
Sbjct: 249 KVKLPGISLFYKSNDEIEAEKLDQGLYDSILRIMEKREKESTMSGEVGSFGTDFLGLLMK 308

Query: 301 AKNDPETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVF 360
           A ND +    I+ +D+VDECKTFY AG ETT  LLAW + LL +H +WQEKAR EVL++F
Sbjct: 309 AMNDADEKNRITAQDVVDECKTFYVAGQETTTTLLAWVIFLLGIHTDWQEKARQEVLNLF 368

Query: 361 GHNNPTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVA 420
           G   P  +GL KLKT+ MIINE LRLYPP + + R+V++E + G L +P    + +PT+A
Sbjct: 369 GQEIPNSDGLAKLKTVNMIINETLRLYPPVIFLTRKVKEETKFGKLTLPANVHIVVPTLA 428

Query: 421 VHHDTTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMS 480
           +HHD   WG+DA  FKPERFS+GV KAT NN+AA+ PFGLGPR+CVG+NFA NEAKIA++
Sbjct: 429 LHHDEQIWGDDALLFKPERFSQGVAKATNNNAAAFFPFGLGPRSCVGLNFATNEAKIALA 488

Query: 481 MILQRYSFRLSPAYAHMPAQLLTISPQNGVQVILNSI 514
           MILQ YSF LSP Y H P Q+LT+ PQ+G+QV+L  +
Sbjct: 489 MILQCYSFALSPTYIHSPVQILTVRPQHGLQVMLQPL 524

[Close]

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot
Match: H1A988 (11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 8.1e-107
Identity = 205/504 (40.67%), Postives = 300/504 (59.52%), Query Frame = 0


Query: 28  LWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMDL---SHNILHRVMPC 87
           LW  P R++R +R+QG+ G  +K    N++  Y+ ++Q  A    +     +   R+   
Sbjct: 32  LWLRPKRLERHLRAQGLHGDPYKLSLDNSKQTYMLKLQQEAQSKSIGLSKDDAAPRIFSL 91

Query: 88  VHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIHRIFGNGLAT 147
            H  ++ YG+N   W G   +++ITDPE IKEV +  Q  FPK KL   I +    GL  
Sbjct: 92  AHQTVHKYGKNSFAWEGTAPKVIITDPEQIKEVFNKIQ-DFPKPKL-NPIAKYISIGLVQ 151

Query: 148 AEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGK----ELDAFKEFKV 207
            EG +WA  R+I N AFH + LK M+P       +MI  WK         E+D +   + 
Sbjct: 152 YEGDKWAKHRKIINPAFHLEKLKGMLPAFSHSCHEMISKWKGLLSSDGTCEVDVWPFLQN 211

Query: 208 FTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKI-----LKSKDDYE 267
            T DVIS TAFGSSY +G KIF +L+       R GY +     ++I     L S     
Sbjct: 212 LTCDVISRTAFGSSYAEGAKIFELLK-------RQGYALMTARYARIPLWWLLPSTTKRR 271

Query: 268 GERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKN----DPETSQSISM-- 327
            + + + ++D    II++RE+ L++G++ D  +D LG+L+++ +      E S+S  M  
Sbjct: 272 MKEIERGIRDSLEGIIRKREKALKSGKSTD--DDLLGILLQSNHIENKGDENSKSAGMTT 331

Query: 328 EDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKL 387
           +++++ECK FY AG ETT  LLAWTM+LL  H EWQ +AR EVL VFG+ NP  EGL +L
Sbjct: 332 QEVMEECKLFYLAGQETTAALLAWTMVLLGKHPEWQARARQEVLQVFGNQNPNFEGLGRL 391

Query: 388 KTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAH 447
           K + MI+ E LRLYPP + + R + K+++LGNL++P    +++P + +HHD   WG DA 
Sbjct: 392 KIVTMILYEVLRLYPPGIYLTRALRKDLKLGNLLLPAGVQVSVPILLIHHDEGIWGNDAK 451

Query: 448 EFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPA 507
           EF PERF+EG+ KAT+     Y PFG GPR CVG NFA+ EAKI +S++LQ +SF LSP 
Sbjct: 452 EFNPERFAEGIAKATK-GQVCYFPFGWGPRICVGQNFALLEAKIVLSLLLQNFSFELSPT 511

Query: 508 YAHMPAQLLTISPQNGVQVILNSI 514
           YAH+P  +LT+ P++G  +IL+ +
Sbjct: 512 YAHVPTTVLTLQPKHGAPIILHKL 523

[Close]

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot
Match: Q2MJ19 (Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 3.1e-106
Identity = 193/496 (38.91%), Postives = 308/496 (62.10%), Query Frame = 0


Query: 28  LWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMDLSHNILHRVMPCVHS 87
           +W  P +I++ +R QG+QG  ++ + G+ +D ++ + +  + PM+LS +I  RV P +H 
Sbjct: 30  MWLKPKKIEKLLREQGLQGNPYRLLLGDAKDYFVMQKKVQSKPMNLSDDIAPRVAPYIHH 89

Query: 88  WLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIHRIFGNGLATAEG 147
            +  +G+    W G+   +++ +PE I+EV  ++   FPK + K    ++   GL   EG
Sbjct: 90  AVQTHGKKSFIWFGMKPWVILNEPEQIREVF-NKMSEFPKVQYK--FMKLITRGLVKLEG 149

Query: 148 QRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGK----ELDAFKEFKVFTL 207
           ++W+  RRI N AFH + LK M PT ++    +I  W+         E+D +   +  T 
Sbjct: 150 EKWSKHRRIINPAFHMEKLKIMTPTFLKSCNDLISNWEKMLSSNGSCEMDVWPSLQSLTS 209

Query: 208 DVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKSKDDYEGERLGKRL 267
           DVI+ ++FGSSY++G+K+F +  E  EL ++N  K  +P + + L + D  +     K++
Sbjct: 210 DVIARSSFGSSYEEGRKVFQLQIEQGELIMKNLMKSLIP-LWRFLPTADHRKINENEKQI 269

Query: 268 KDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKA------KNDPETSQSISMEDIVDECK 327
           +     II +RE+ ++ GEA +  ND LGLL+++      ++    +  +S+E++V EC+
Sbjct: 270 ETTLKNIINKREKAIKAGEATE--NDLLGLLLESNHREIKEHGNVKNMGLSLEEVVGECR 329

Query: 328 TFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLKTMAMIIN 387
            F+ AG ETT+ LL WTM+LL+ + +WQE+AR EVL++FG+  P  +GL KLK MAMI+ 
Sbjct: 330 LFHVAGQETTSDLLVWTMVLLSRYPDWQERARKEVLEIFGNEKPDFDGLNKLKIMAMILY 389

Query: 388 ECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAHEFKPERFS 447
           E LRLYPP   VAR+VE +++LG+L +     + +P V +HHD   WG+DA  F PERFS
Sbjct: 390 EVLRLYPPVTGVARKVENDIKLGDLTLYAGMEVYMPIVLIHHDCELWGDDAKIFNPERFS 449

Query: 448 EGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPAYAHMPAQL 507
            G+ KAT N   +Y PFG GPR C+G NF++ EAK+AM++IL+ +SF LS  YAH P+ +
Sbjct: 450 GGISKAT-NGRFSYFPFGAGPRICIGQNFSLLEAKMAMALILKNFSFELSQTYAHAPSVV 509

Query: 508 LTISPQNGVQVILNSI 514
           L++ PQ+G  VIL  I
Sbjct: 510 LSVQPQHGAHVILRKI 518

[Close]

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot
Match: Q2MJ21 (Cytochrome P450 716A67 OS=Medicago truncatula OX=3880 GN=CYP72A67 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 5.3e-106
Identity = 206/525 (39.24%), Postives = 314/525 (59.81%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVF--KLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRD 60
           ME ++  Y  I L    L  V+  ++   +W  P R+++ +R QG  G S++ + G+ +D
Sbjct: 1   MEASLAIYYGIILITVTLGLVYTWRVLNWIWLKPKRLEKLLREQGCNGNSYRLVLGDLKD 60

Query: 61  VYIKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVL 120
            Y    +A + PM+LS +I+ RV+P +   + +YG+N   WSG   +L++T+PE+IK+VL
Sbjct: 61  SYKMGKKAKSKPMELSDDIIPRVIPYIQQLVQIYGKNPFIWSGTTPRLILTEPELIKDVL 120

Query: 121 HDRQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGE 180
           +   ++    K K  I +   +GL   EG++W   RR+ N AF  + LK M P+ +    
Sbjct: 121 N---RTSELQKPKYEIFKFLFSGLIIHEGEKWRKHRRLMNAAFQLEKLKIMAPSFLTSCI 180

Query: 181 KMIEGWK---NYEGK-ELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIR 240
            MI  W+   + +G  E+D +   +  T DVIS  AFGSSY++GK+IF + RE  EL ++
Sbjct: 181 DMISKWESTLSSDGSGEIDIWPSLQNLTSDVISRNAFGSSYEEGKRIFDLQREQGELVMK 240

Query: 241 NGYKIRLPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLL 300
           N  K  +P + + + +        + K +      II +RE+ ++ GEA +  ND LGLL
Sbjct: 241 NLVKSLIP-LWRFIPTATQRRMHEIEKDIDSSLRYIINKREKAMKAGEATE--NDLLGLL 300

Query: 301 IKAKNDP------ETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKA 360
           +++ +          +  +S+ED+V ECK FY AG E+T+ +L WTM+LL+ + +WQE+A
Sbjct: 301 LESNHQEIRDHGNNKNMGMSLEDVVGECKLFYLAGQESTSTMLVWTMILLSRYPDWQERA 360

Query: 361 RNEVLDVFGHNNPTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTAT 420
           R EVL +FG+  P  EGL KLK + MI+ E LRLYPPA  V R V K+++ GN+ VP   
Sbjct: 361 REEVLQIFGNKKPDYEGLNKLKILPMILYEVLRLYPPAFGVTRYVGKDIKFGNMEVPAGV 420

Query: 421 MLTIPTVAVHHDTTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAM 480
            + +P + + H+   WG+DA  F PERF+EG+ KAT N    Y PFG GPR C+G NF++
Sbjct: 421 EVFLPIILLQHNNELWGDDAKMFNPERFAEGISKAT-NGRFIYFPFGGGPRVCMGQNFSL 480

Query: 481 NEAKIAMSMILQRYSFRLSPAYAHMPAQLLTISPQNGVQVILNSI 514
            EAK+A+SMILQ + F LSP YAH P  ++TI P+ G  VIL  +
Sbjct: 481 LEAKMAVSMILQNFYFELSPTYAHTPNLVMTIQPEKGAHVILRKV 518

[Close]

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 9.0e-106
Identity = 195/501 (38.92%), Postives = 316/501 (63.07%), Query Frame = 0


Query: 22  FKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPMDLSHNILHRV 81
           +++F  +W  P ++++++R+QG  G S++   G+ +++ +   +A + P++L  +I+ R+
Sbjct: 22  WRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEAKSKPINLYDDIIPRI 81

Query: 82  MPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLKGHIHRIFGNG 141
           +P     +  YG+N   W G    + I +P+ IK+VL  +   F K +    + ++   G
Sbjct: 82  IPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVL-SKFYQFQKPR-HNPLTKLLATG 141

Query: 142 LATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWK---NYEGK-ELDAFKE 201
           +A AEG RWA  R++ N AFH + LKNM+P +     +++  W+   + +G+ ELD    
Sbjct: 142 VADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQFELDVLPY 201

Query: 202 FKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKSKDDYEGE 261
            +  T DVIS TAFGSSY++G+KIF + RE  EL I+    I LP + + L +K +   +
Sbjct: 202 LETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGM-RFLPTKRNKRMK 261

Query: 262 RLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKA-----KNDPETSQSISMEDI 321
            + K +K     II +R + +  GE + + +D LG+L+++     K    T+  ++++++
Sbjct: 262 EIAKEVKIALKSIINKRLKAMEAGERSSH-DDLLGILLESNSKEIKQHGNTNFGLTVDEV 321

Query: 322 VDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLKTM 381
           ++ECK F+FAG ETT+ LL WTM+LL+ H++WQ++A+ EVL  FG+N P  +GL  LK +
Sbjct: 322 IEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDFDGLNHLKVV 381

Query: 382 AMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDTTFWGEDAHEFK 441
            MI+ E LRLYPP + + R + +E++LG + +P   +L +P + +H+D   WG+DA EF 
Sbjct: 382 NMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWGDDAKEFN 441

Query: 442 PERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQRYSFRLSPAYAH 501
           PERFSEGV KAT+     Y PF  GPR C+G NFAM EAK+AM+MILQR+SF LSP+YAH
Sbjct: 442 PERFSEGVLKATKGR-VTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVLSPSYAH 501

Query: 502 MPAQLLTISPQNGVQVILNSI 514
            P  ++T+ PQ G  +IL+S+
Sbjct: 502 APHAIITLQPQYGAHLILHSL 517

[Close]

BLAST of Cucsat.G9472 vs. NCBI nr
Match: XP_004135430.2 (cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetical protein Csa_008223 [Cucumis sativus])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 514/515 (99.81%), Postives = 514/515 (99.81%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY
Sbjct: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
           IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD
Sbjct: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM
Sbjct: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR
Sbjct: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND
Sbjct: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PETSQ ISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN
Sbjct: 301 PETSQCISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD
Sbjct: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
           TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ
Sbjct: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD
Sbjct: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515

[Close]

BLAST of Cucsat.G9472 vs. NCBI nr
Match: XP_031742247.1 (cytochrome P450 CYP749A22 isoform X2 [Cucumis sativus])

HSP 1 Score: 1008 bits (2605), Expect = 0.0
Identity = 497/515 (96.50%), Postives = 497/515 (96.50%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY
Sbjct: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
           IKRMQAMATPMDLSHNILHRVMPCVHSWLNLY                 DPEMIKEVLHD
Sbjct: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLY-----------------DPEMIKEVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM
Sbjct: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR
Sbjct: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND
Sbjct: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PETSQ ISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN
Sbjct: 301 PETSQCISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD
Sbjct: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
           TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ
Sbjct: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD
Sbjct: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 498

[Close]

BLAST of Cucsat.G9472 vs. NCBI nr
Match: XP_008446466.1 (PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo])

HSP 1 Score: 995 bits (2573), Expect = 0.0
Identity = 484/515 (93.98%), Postives = 497/515 (96.50%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           MECTII YISIFLSGCLLF VFKLFVKLWWTPMRIQRFMRSQGIQGPS+KFIQGNTRDVY
Sbjct: 1   MECTIIIYISIFLSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
           +KRMQAMATPMDLSHNIL RVMP VHSWLNLYGRNYLQW+GVDAQLMITDPEMIKEVLHD
Sbjct: 61  MKRMQAMATPMDLSHNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQKSFPKAKLKGHI RIFGNGLATAEGQRW NSRRIANFAFHGDSLKNMIPTMIECGEKM
Sbjct: 121 RQKSFPKAKLKGHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHML+ELC+LSIRNGYKI+
Sbjct: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIK 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKILKSKDDYEGERL KRLKDCFMEIIKEREEKLRN EA+DYGNDFLGLLIKAKND
Sbjct: 241 LPIISKILKSKDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEV DVFGHN+
Sbjct: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNH 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PTLEGLPKLK MAMIINECLRLYPPAM +ARRVEKEVRLGNLVVPT T+L IPTVAVHHD
Sbjct: 361 PTLEGLPKLKAMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHD 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
           T FWG+DAHEFKPERFSEG+GKATE NSAAY+PFGLGPRNCVGMNFA NEAKIAMSMILQ
Sbjct: 421 TKFWGDDAHEFKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           RYSF LSPAYAHMPAQLLTI PQNGVQVILNSIAD
Sbjct: 481 RYSFSLSPAYAHMPAQLLTICPQNGVQVILNSIAD 515

[Close]

BLAST of Cucsat.G9472 vs. NCBI nr
Match: KAA0034483.1 (cytochrome P450 CYP749A22-like [Cucumis melo var. makuwa] >TYK09037.1 cytochrome P450 CYP749A22-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 467/514 (90.86%), Postives = 483/514 (93.97%), Query Frame = 0


Query: 12  FLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPM 71
           FL+ C   G+ +LFVKLWWTPMRIQRFMRSQGIQGPS+KFIQGNTRDVY+KRMQAMATPM
Sbjct: 417 FLTTCPQQGL-QLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPM 476

Query: 72  DLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 131
           DLSHNIL RVMP VHSWLNLYGRNYLQW+GVDAQLMITDPEMIKEVLHDRQKSFPKAKLK
Sbjct: 477 DLSHNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 536

Query: 132 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE 191
           GHI RIFGNGLATAEGQRW NSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE
Sbjct: 537 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE 596

Query: 192 LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKSK 251
           LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHML+ELC+LSIRNGYKI+LPIISKILKSK
Sbjct: 597 LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKSK 656

Query: 252 DDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQSISMED 311
           DDYEGERL KRLKDCFMEIIKEREEKLRN EA+DYGNDFLGLLIKAKNDPETSQSISMED
Sbjct: 657 DDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISMED 716

Query: 312 IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLKT 371
           IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEV DVFGHN+PTLEGLPKLK 
Sbjct: 717 IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLKA 776

Query: 372 ----------MAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDT 431
                     MAMIINECLRLYPPAM +ARRVEKEVRLGNLVVPT T+L IPTVAVHHDT
Sbjct: 777 DVCVRFYYGQMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDT 836

Query: 432 TFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQR 491
            FWG+DAHEFKPERFSEG+GKATE NSAAY+PFGLGPRNCVGMNFA NEAKIAMSMILQR
Sbjct: 837 KFWGDDAHEFKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQR 896

Query: 492 YSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           YSF LSPAYAHMPAQLLTI PQNGVQVILNSIAD
Sbjct: 897 YSFSLSPAYAHMPAQLLTICPQNGVQVILNSIAD 929

[Close]

BLAST of Cucsat.G9472 vs. NCBI nr
Match: XP_038892924.1 (cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida])

HSP 1 Score: 946 bits (2445), Expect = 0.0
Identity = 460/513 (89.67%), Postives = 480/513 (93.57%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           MEC II YIS FL GCLLFGV KLFVKLWWTPMRIQRFMRSQGIQGPS+KFIQGNTRDVY
Sbjct: 1   MECKIIIYISTFLFGCLLFGVIKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
            KRMQAMATPMDLSH+IL RVMP VHSWLNLYGRN+LQW GVDAQL+ITDPEMIKEVLHD
Sbjct: 61  TKRMQAMATPMDLSHHILPRVMPSVHSWLNLYGRNFLQWFGVDAQLIITDPEMIKEVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQK+FPKAKL+GHIHRIFGNGLATAEG+RWA SR+IANFAFHGDSLKNMIPTMIECGEKM
Sbjct: 121 RQKNFPKAKLQGHIHRIFGNGLATAEGERWAKSRKIANFAFHGDSLKNMIPTMIECGEKM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
            EGWKNYEGKELD FKEFKV+TLDVISHTAFGSSYQQGK IFHML+ELCELSIRNGYKI+
Sbjct: 181 TEGWKNYEGKELDVFKEFKVYTLDVISHTAFGSSYQQGKDIFHMLQELCELSIRNGYKIK 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKILKSKDDYEGERL KR+KDCFMEIIK REEKLRNGEA+ YGNDFLGLL+KAKND
Sbjct: 241 LPIISKILKSKDDYEGERLEKRMKDCFMEIIKGREEKLRNGEADGYGNDFLGLLVKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PE SQ IS+EDIVDECKTFYFAGHETTNVLLAWTM LLALHKEWQE+ARNEVL VFG  N
Sbjct: 301 PEISQRISVEDIVDECKTFYFAGHETTNVLLAWTMFLLALHKEWQEEARNEVLKVFGDKN 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PTLEGLPKL+ M +IINECLRLYPPAM V+R VEKEVRLGNLVVPT T L IPTVAVHHD
Sbjct: 361 PTLEGLPKLRMMMIIINECLRLYPPAMTVSRLVEKEVRLGNLVVPTMTKLVIPTVAVHHD 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
            TFWGEDAHEFKPERF EGVGKATE+NSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ
Sbjct: 421 MTFWGEDAHEFKPERFLEGVGKATESNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSI 513
           RYSF LSPAY HMPAQLLTISPQNGVQVILNSI
Sbjct: 481 RYSFDLSPAYVHMPAQLLTISPQNGVQVILNSI 513

[Close]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL
Match: A0A1S3BF46 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1)

HSP 1 Score: 995 bits (2573), Expect = 0.0
Identity = 484/515 (93.98%), Postives = 497/515 (96.50%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           MECTII YISIFLSGCLLF VFKLFVKLWWTPMRIQRFMRSQGIQGPS+KFIQGNTRDVY
Sbjct: 1   MECTIIIYISIFLSGCLLFVVFKLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
           +KRMQAMATPMDLSHNIL RVMP VHSWLNLYGRNYLQW+GVDAQLMITDPEMIKEVLHD
Sbjct: 61  MKRMQAMATPMDLSHNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQKSFPKAKLKGHI RIFGNGLATAEGQRW NSRRIANFAFHGDSLKNMIPTMIECGEKM
Sbjct: 121 RQKSFPKAKLKGHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHML+ELC+LSIRNGYKI+
Sbjct: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIK 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKILKSKDDYEGERL KRLKDCFMEIIKEREEKLRN EA+DYGNDFLGLLIKAKND
Sbjct: 241 LPIISKILKSKDDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEV DVFGHN+
Sbjct: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNH 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PTLEGLPKLK MAMIINECLRLYPPAM +ARRVEKEVRLGNLVVPT T+L IPTVAVHHD
Sbjct: 361 PTLEGLPKLKAMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHD 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
           T FWG+DAHEFKPERFSEG+GKATE NSAAY+PFGLGPRNCVGMNFA NEAKIAMSMILQ
Sbjct: 421 TKFWGDDAHEFKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           RYSF LSPAYAHMPAQLLTI PQNGVQVILNSIAD
Sbjct: 481 RYSFSLSPAYAHMPAQLLTICPQNGVQVILNSIAD 515

[Close]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL
Match: A0A5D3CAL6 (Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00230 PE=4 SV=1)

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 467/514 (90.86%), Postives = 483/514 (93.97%), Query Frame = 0


Query: 12  FLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVYIKRMQAMATPM 71
           FL+ C   G+ +LFVKLWWTPMRIQRFMRSQGIQGPS+KFIQGNTRDVY+KRMQAMATPM
Sbjct: 417 FLTTCPQQGL-QLFVKLWWTPMRIQRFMRSQGIQGPSYKFIQGNTRDVYMKRMQAMATPM 476

Query: 72  DLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 131
           DLSHNIL RVMP VHSWLNLYGRNYLQW+GVDAQLMITDPEMIKEVLHDRQKSFPKAKLK
Sbjct: 477 DLSHNILPRVMPSVHSWLNLYGRNYLQWAGVDAQLMITDPEMIKEVLHDRQKSFPKAKLK 536

Query: 132 GHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE 191
           GHI RIFGNGLATAEGQRW NSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE
Sbjct: 537 GHIDRIFGNGLATAEGQRWVNSRRIANFAFHGDSLKNMIPTMIECGEKMIEGWKNYEGKE 596

Query: 192 LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIRLPIISKILKSK 251
           LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHML+ELC+LSIRNGYKI+LPIISKILKSK
Sbjct: 597 LDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLQELCDLSIRNGYKIKLPIISKILKSK 656

Query: 252 DDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKNDPETSQSISMED 311
           DDYEGERL KRLKDCFMEIIKEREEKLRN EA+DYGNDFLGLLIKAKNDPETSQSISMED
Sbjct: 657 DDYEGERLEKRLKDCFMEIIKEREEKLRNDEASDYGNDFLGLLIKAKNDPETSQSISMED 716

Query: 312 IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNNPTLEGLPKLKT 371
           IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEV DVFGHN+PTLEGLPKLK 
Sbjct: 717 IVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVFDVFGHNHPTLEGLPKLKA 776

Query: 372 ----------MAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHDT 431
                     MAMIINECLRLYPPAM +ARRVEKEVRLGNLVVPT T+L IPTVAVHHDT
Sbjct: 777 DVCVRFYYGQMAMIINECLRLYPPAMTIARRVEKEVRLGNLVVPTTTLLAIPTVAVHHDT 836

Query: 432 TFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQR 491
            FWG+DAHEFKPERFSEG+GKATE NSAAY+PFGLGPRNCVGMNFA NEAKIAMSMILQR
Sbjct: 837 KFWGDDAHEFKPERFSEGIGKATEKNSAAYLPFGLGPRNCVGMNFATNEAKIAMSMILQR 896

Query: 492 YSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           YSF LSPAYAHMPAQLLTI PQNGVQVILNSIAD
Sbjct: 897 YSFSLSPAYAHMPAQLLTICPQNGVQVILNSIAD 929

[Close]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL
Match: A0A6J1D6R8 (cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE=3 SV=1)

HSP 1 Score: 798 bits (2062), Expect = 6.79e-288
Identity = 381/516 (73.84%), Postives = 438/516 (84.88%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           M+  I  Y+S F+ GCLLFG  KLF+KLWWTP+RIQRFMRSQGIQGPS+ FIQGN R+++
Sbjct: 1   MKMNINIYVSTFVFGCLLFGTIKLFIKLWWTPIRIQRFMRSQGIQGPSYNFIQGNAREMH 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
            +R  A+ATP+DLSH IL RV+P  HSWL+ YGRN++QW GV+AQL+ITDPEMI+EVLHD
Sbjct: 61  TRRTHAIATPIDLSHRILPRVLPHTHSWLHRYGRNFIQWFGVEAQLIITDPEMIREVLHD 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQK FPKAKL G +++IFGNGL TAEG+ WA SR+IANFAFHGD+LKNMIP MI C E M
Sbjct: 121 RQKKFPKAKLHGFLNKIFGNGLVTAEGETWAKSRKIANFAFHGDTLKNMIPAMIGCAETM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           +E WK+YEGKELD F+  KV+T DVISHTAFGSSY+QGK IF ML+ LCEL IRNG+KIR
Sbjct: 181 MEEWKHYEGKELDVFQHLKVYTSDVISHTAFGSSYEQGKNIFQMLQNLCELIIRNGFKIR 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LP I KI KSKDD EGERL KR+KDCFME+IK REEK  NGEA+ YGNDFLGLL+KAKND
Sbjct: 241 LPGIRKIFKSKDDVEGERLEKRMKDCFMEVIKSREEKFINGEADGYGNDFLGLLVKAKND 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PE SQ IS++DIVDECKTFYFAGHETT+VLLAWTM LLALHK+WQ++AR+EV  VFG  N
Sbjct: 301 PEESQRISVDDIVDECKTFYFAGHETTSVLLAWTMFLLALHKQWQDQARDEVFKVFGDKN 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRV-EKEVRLGNLVVPTATMLTIPTVAVHH 420
           PT EGLPKLKTM MI+ ECLRLYPPAM V R+V EKEVRLG+LV+P    +TIPT AVHH
Sbjct: 361 PTSEGLPKLKTMTMILYECLRLYPPAMTVGRQVMEKEVRLGSLVLPATIRVTIPTAAVHH 420

Query: 421 DTTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMIL 480
           DT +WGEDA EFKPERF+EGV KA E NSAAY+PFGLG R+CVGMNFAM EAKI MSMIL
Sbjct: 421 DTAYWGEDASEFKPERFAEGVAKAIERNSAAYLPFGLGGRSCVGMNFAMKEAKIGMSMIL 480

Query: 481 QRYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           QRYSF LSPAYAH P Q LTI PQ+G+Q+IL+SI+D
Sbjct: 481 QRYSFTLSPAYAHSPVQFLTICPQHGLQLILHSISD 516

[Close]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL
Match: A0A6J1HWK2 (cytochrome P450 CYP749A22-like OS=Cucurbita maxima OX=3661 GN=LOC111467508 PE=3 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 1.90e-281
Identity = 363/513 (70.76%), Postives = 439/513 (85.58%), Query Frame = 0


Query: 1   MECTIINYISIFLSGCLLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNTRDVY 60
           ME  + N IS+FL   +  G+FKL  KLWWTP++IQRFMRSQGIQGPS+ FIQGNTR++Y
Sbjct: 1   MEDVMSNTISVFLIASVALGIFKLLDKLWWTPIKIQRFMRSQGIQGPSYHFIQGNTREMY 60

Query: 61  IKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKEVLHD 120
            KRM AMA+P+DLSH IL RV+P  HSWLN YGRN++QW G DA L+IT+PEMIKEVL+D
Sbjct: 61  SKRMTAMASPIDLSHRILPRVLPHTHSWLNDYGRNFVQWFGTDAHLVITEPEMIKEVLND 120

Query: 121 RQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIECGEKM 180
           RQK+FPKAKL+GH+HRIFGNGL TA+G RW  SR++A+FAFHGD+LKNMIP+MIEC E M
Sbjct: 121 RQKNFPKAKLQGHVHRIFGNGLVTAQGDRWVKSRKLAHFAFHGDNLKNMIPSMIECAETM 180

Query: 181 IEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNGYKIR 240
           +E WK++  KELD F+ FKV+TLDVISHTAFGSSY+QG+  F ML+ +CELS+RNGYK+R
Sbjct: 181 VEEWKHHIDKELDVFEHFKVYTLDVISHTAFGSSYEQGRSFFQMLQRVCELSVRNGYKVR 240

Query: 241 LPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIKAKND 300
           LPIISKI KSKDD EGERL KR+++CF+EII+ REEKL NGEA  YGNDFLGLL+KAKN+
Sbjct: 241 LPIISKIFKSKDDVEGERLEKRMRECFIEIIRTREEKLMNGEAEGYGNDFLGLLVKAKNE 300

Query: 301 PETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVFGHNN 360
           PE +Q +S++DIVDECKTFYFAGHETTN LLAWTM LLALH+EWQE+AR EV  V G  N
Sbjct: 301 PEKAQRLSVDDIVDECKTFYFAGHETTNALLAWTMFLLALHREWQEEARKEVFKVLGDKN 360

Query: 361 PTLEGLPKLKTMAMIINECLRLYPPAMPVARRVEKEVRLGNLVVPTATMLTIPTVAVHHD 420
           PT+E LPKL+TM MIINECLRLYPPAM ++R+V+KE RLG+LV+P+   + IPT+AV H+
Sbjct: 361 PTMESLPKLRTMNMIINECLRLYPPAMTLSRQVQKETRLGSLVLPSRIRVLIPTLAVQHN 420

Query: 421 TTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIAMSMILQ 480
           T  WGEDA  FKPERF+EGVGKATE NS AY+PFGLGPR+CVGMNFA+NEAKIA+S+ILQ
Sbjct: 421 TELWGEDADVFKPERFAEGVGKATETNSGAYLPFGLGPRSCVGMNFALNEAKIALSIILQ 480

Query: 481 RYSFRLSPAYAHMPAQLLTISPQNGVQVILNSI 513
           R+SF LSP+Y+H P Q LTI+PQ+GVQ+IL+ +
Sbjct: 481 RFSFTLSPSYSHSPIQFLTINPQHGVQLILHPV 513

[Close]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL
Match: A0A1S3BEM5 (cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489194 PE=3 SV=1)

HSP 1 Score: 781 bits (2018), Expect = 4.71e-281
Identity = 382/521 (73.32%), Postives = 439/521 (84.26%), Query Frame = 0


Query: 1   MECTIINYISI--FLSGC--LLFGVFKLFVKLWWTPMRIQRFMRSQGIQGPSFKFIQGNT 60
           M+  I  YI I   + GC  L+  + KL  K WWTPMRIQR MRSQGI GPS+KFIQGN 
Sbjct: 10  MDVVISKYIIIPSLVFGCVILMSSIMKLVNKFWWTPMRIQRIMRSQGINGPSYKFIQGNM 69

Query: 61  RDVYIKRMQAMATPMDLSHNILHRVMPCVHSWLNLYGRNYLQWSGVDAQLMITDPEMIKE 120
           RD+Y KRMQAMATPM+LSHNIL RV+P VHSWLN YGR++LQW G++AQL+ITDPEMIKE
Sbjct: 70  RDMYTKRMQAMATPMELSHNILPRVIPHVHSWLNDYGRSFLQWYGMEAQLIITDPEMIKE 129

Query: 121 VLHDRQKSFPKAKLKGHIHRIFGNGLATAEGQRWANSRRIANFAFHGDSLKNMIPTMIEC 180
           VL+D++K++PKAKL   + RIFGNGL T+EGQRWA SR+IANFAFHGDSLKNMIPTMIEC
Sbjct: 130 VLNDKRKNYPKAKLGSDLFRIFGNGLVTSEGQRWAKSRKIANFAFHGDSLKNMIPTMIEC 189

Query: 181 GEKMIEGWKNYEGKELDAFKEFKVFTLDVISHTAFGSSYQQGKKIFHMLRELCELSIRNG 240
           GEKMIEGWKNYEGKELD FKE KV+TLDVISHTAFGSSYQQG  IFHML++L +LSIRNG
Sbjct: 190 GEKMIEGWKNYEGKELDVFKELKVYTLDVISHTAFGSSYQQGSNIFHMLQQLTDLSIRNG 249

Query: 241 YKIRLPIISKILKSKDDYEGERLGKRLKDCFMEIIKEREEKLRNGEANDYGNDFLGLLIK 300
           YKI+LPIISKILKSKDD EGERL KR+ +CF EII+ REEK + GE   YGNDFLG+L+K
Sbjct: 250 YKIKLPIISKILKSKDDIEGERLEKRMNECFAEIIRGREEKSKKGEG--YGNDFLGMLVK 309

Query: 301 AKNDPETSQSISMEDIVDECKTFYFAGHETTNVLLAWTMLLLALHKEWQEKARNEVLDVF 360
           AKN+ E S+ I+M+ IV ECKTFYFAGHETTNVL+ W + LLA+HKEWQE+ARNEV  +F
Sbjct: 310 AKNEGEKSERITMDVIVAECKTFYFAGHETTNVLITWIIFLLAIHKEWQEQARNEVFRIF 369

Query: 361 GHNN-PTLEGLPKLKTMAMIINECLRLYPPAMPVARRV-EKEVRLGNLVVPTATMLTIPT 420
           GHNN PT E L KLK M MIINE LRLYPPAM V+R+V EKEV+LG+LV+PT+  LTIPT
Sbjct: 370 GHNNNPTYEALSKLKIMTMIINETLRLYPPAMTVSRQVVEKEVKLGSLVLPTSLQLTIPT 429

Query: 421 VAVHHDTTFWGEDAHEFKPERFSEGVGKATENNSAAYIPFGLGPRNCVGMNFAMNEAKIA 480
           +AVHHD  FWGED HEFKPERFSEGV K  E NSA Y+PFGLGPRNCVGMNFA+NEAK+A
Sbjct: 430 IAVHHDKEFWGEDVHEFKPERFSEGVSKTIERNSAGYLPFGLGPRNCVGMNFAINEAKVA 489

Query: 481 MSMILQRYSFRLSPAYAHMPAQLLTISPQNGVQVILNSIAD 515
           MS+ILQ+YSF LSPAYAH P Q LT  PQ G+QVIL+SI++
Sbjct: 490 MSIILQKYSFTLSPAYAHTPVQFLTTCPQQGLQVILHSISN 528

The following BLAST results are available for this feature:

BLAST of Cucsat.G9472 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H2DH17	6.8e-154	50.48	Cytochrome P450 CYP749A22 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
H1A988	8.1e-107	40.67	11-oxo-beta-amyrin 30-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP72A154 PE=...	[more]
Q2MJ19	3.1e-106	38.91	Cytochrome P450 72A68 OS=Medicago truncatula OX=3880 GN=CYP72A68 PE=1 SV=1	[more]
Q2MJ21	5.3e-106	39.24	Cytochrome P450 716A67 OS=Medicago truncatula OX=3880 GN=CYP72A67 PE=2 SV=1	[more]
H2DH21	9.0e-106	38.92	Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]

BLAST of Cucsat.G9472 vs. NCBI nr


Match Name	Identity	Description
XP_004135430.2	99.81	cytochrome P450 CYP749A22 isoform X1 [Cucumis sativus] >KAE8648770.1 hypothetica...	[more]
XP_031742247.1	96.50	cytochrome P450 CYP749A22 isoform X2 [Cucumis sativus]	[more]
XP_008446466.1	93.98	PREDICTED: cytochrome P450 CYP749A22-like [Cucumis melo]	[more]
KAA0034483.1	90.86	cytochrome P450 CYP749A22-like [Cucumis melo var. makuwa] >TYK09037.1 cytochrome...	[more]
XP_038892924.1	89.67	cytochrome P450 CYP749A22-like isoform X1 [Benincasa hispida]	[more]

BLAST of Cucsat.G9472 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BF46	0.0	93.98	cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489195 PE=3 SV=1	[more]
A0A5D3CAL6	0.0	90.86	Cytochrome P450 CYP749A22-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1D6R8	6.79e-288	73.84	cytochrome P450 CYP749A22-like OS=Momordica charantia OX=3673 GN=LOC111017564 PE...	[more]
A0A6J1HWK2	1.90e-281	70.76	cytochrome P450 CYP749A22-like OS=Cucurbita maxima OX=3661 GN=LOC111467508 PE=3 ...	[more]
A0A1S3BEM5	4.71e-281	73.32	cytochrome P450 CYP749A22-like OS=Cucumis melo OX=3656 GN=LOC103489194 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 163..189 score: 31.8 coord: 246..270 score: 22.43 coord: 143..160 score: 26.08 coord: 293..316 score: 28.81 coord: 33..51 score: 20.36 coord: 283..293 score: 51.62
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 154..171 score: 40.21 coord: 293..304 score: 39.8 coord: 206..217 score: 41.95 coord: 284..293 score: 60.84
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..321 e-value: 3.3E-71 score: 240.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..346 e-value: 2.1E-94 score: 318.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..342
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 1..342
None	No IPR available	PANTHER	PTHR24282:SF20	CYTOCHROME P450 CYP749A22-LIKE	coord: 1..342
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 286..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G9472.T1	Cucsat.G9472.T1	mRNA
Cucsat.G9472.T4	Cucsat.G9472.T4	mRNA
Cucsat.G9472.T3	Cucsat.G9472.T3	mRNA
Cucsat.G9472.T2	Cucsat.G9472.T2	mRNA
Cucsat.G9472.T5	Cucsat.G9472.T5	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen