Cucsat.G9435 (gene) Cucumber (B10) v3

BLAST of Cucsat.G9435 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.8e-97
Identity = 172/332 (51.81%), Postives = 229/332 (68.98%), Query Frame = 0

Query: 22  NGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLNNIWAR 81
           N  + H  +V+E+EGLI+VY +G+VER Q++PCV  +LP ELGVT  DVV+DKL N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 82  FYIPTQCQE----KLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKANCIIMSVNYRLAP 141
            Y+P    +    KLPLIVYFHGGGFCVGSA+W CYHEFLA+LSA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 142 ENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAARLG 201
           ENPLPA YEDG+  + WL +        N W++ CDF +I+L+GDSAGGNIA  VAARL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNKAR----NDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 202 GKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTLGVSDTYWRLALP 261
                +       L I+G+ILIQPF+ GE RT+SE+ +     + LTL  SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 262 SGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSALHRAGKLINYVV 321
            G NR+HP+C P       ++   V  +L+C++EMD+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNE---DVIKRVL 314

Query: 322 YEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFL 350
           ++GVGHAF +L KSQL+ T TLEM+  I AF+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Cucsat.G9435 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-86
Identity = 162/330 (49.09%), Postives = 217/330 (65.76%), Query Frame = 0

Query: 26  QHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGVTSWDVVVDKLNNIWARFYIP 85
           +H  +VEEIEGLI+V+ +G VERP IVP VS  + P    T++D+ +   N+ W R YIP
Sbjct: 24  RHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLS--NDTWTRVYIP 83

Query: 86  TQCQEK----LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKANCIIMSVNYRLAPENPL 145
                     LPL+VYFHGGGFCVGSAAWSCYH+FL  L+ KA C+I+SVNYRLAPE+ L
Sbjct: 84  DAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRL 143

Query: 146 PAPYEDGLKTLQWL--KQVAFVGGKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAARLGGK 205
           PA Y+DG+  + WL  +Q++  GG  +W S+ C+ + ++L+GDSAG NIA+ VA R+   
Sbjct: 144 PAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVRI--- 203

Query: 206 TTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTLGVSDTYWRLALPSG 265
             ASG      L +KG ILI PFFGGESRT SEK       S LTL  SD YWRLALP G
Sbjct: 204 -MASGK-YANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRG 263

Query: 266 TNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSALHRAGKLINYVVYE 325
            +RDHPWCNP    L +    ++  +++ ++E DILK+RNLE C  +   GK +  +V+ 
Sbjct: 264 ASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHG 323

Query: 326 GVGHAFQVLNKSQLSQTRTLEMIDHIKAFL 350
           GVGHAF +L+ S +S+ R  +M+  +  F+
Sbjct: 324 GVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Cucsat.G9435 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 5.4e-54
Identity = 113/320 (35.31%), Postives = 178/320 (55.62%), Query Frame = 0

Query: 30  LVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGVTSW--DVVVDKLNNIWARFYIPTQ 89
           + E+  GL+++  NG V R + +  ++  +P +   T    D +  K NN+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 90  CQEK--LPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKANCIIMSVNYRLAPENPLPAPY 149
              +  LP++V+FHGGGFC GS +W  +H F   L++  N +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 150 EDGLKTLQWLKQVAFVGGKQNWW--SRYCDFTKIYLSGDSAGGNIAFNVAARLGGKTTAS 209
           ED    L WL   A   G  +W+      DF ++++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 210 GAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTLGVSDTYWRLALPSGTNRD 269
           G++ L P+ ++G +L+ PFFGGE RT SE     P  + L+L + D +WRL+LP+G  RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 270 HPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSALHR-AGKLINYVVYEGVG 329
           H   NP      T+E + + P L+ +   ++L+DR  E+   L +  GK ++Y+ +E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 330 HAFQVLNKSQLSQTRTLEMI 343
           H F     S  +  + L +I
Sbjct: 310 HGFYSNYPSSEAAEQVLRII 321

BLAST of Cucsat.G9435 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 106/301 (35.22%), Postives = 165/301 (54.82%), Query Frame = 0

Query: 46  VERPQIVPCVSNALPPELG--VTSWDVVVDKLNNIWARFYIPTQC---QEKLPLIVYFHG 105
           + RP  +P  + +  P     V + D+ ++ L+N + R ++P        KLPL+VYFHG
Sbjct: 32  ITRPIQIPSTAASPDPTSSSPVLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHG 91

Query: 106 GGFCVGSAAWSCYHEFLAKLSAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF 165
           GGF + SAA + +H+F  +++  A  +I SV+YRLAPE+ LPA Y+D ++ LQW+K    
Sbjct: 92  GGFILFSAASTIFHDFCCEMAVHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD--- 151

Query: 166 VGGKQNWWSRYCDFTKIYLSGDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQ 225
              +  W + + DF+  ++ G+SAGGNIA++   R      A+ A  L PL IKG +L +
Sbjct: 152 --SRDEWLTNFADFSNCFIMGESAGGNIAYHAGLR-----AAAVADELLPLKIKGLVLDE 211

Query: 226 PFFGGESRTKSEKFLVQPPRSPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKG--LFTVE 285
           P FGG  RT SE  L    R P    V D  W L+LP G +RDH +CNP+ +   L++ +
Sbjct: 212 PGFGGSKRTGSELRLANDSRLPTF--VLDLIWELSLPMGADRDHEYCNPTAESEPLYSFD 271

Query: 286 DLRVLPSLICI--SEMDILKDRNLEFCSALHRAGKLINYVVYEGVG--HAFQVLNKSQLS 336
            +R L   + +     D + DR +E    L + G  ++ V    VG  HA ++ +  +  
Sbjct: 272 KIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKG--VDVVAQFDVGGYHAVKLEDPEKAK 318

BLAST of Cucsat.G9435 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 5.3e-41
Identity = 110/356 (30.90%), Postives = 175/356 (49.16%), Query Frame = 0

Query: 2   RTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVER---PQIVPCVSNA 61
           ++ R P+ +L   P+  +        D +V +++  + +Y +G +ER     ++P     
Sbjct: 63  KSSRSPSPSLSPTPTALD--------DEIVLDLKPFLIIYKSGRIERFLGTTVIPAC--- 122

Query: 62  LPPELGVTSWDVVVDKLNNIWARFYIPTQC---QEKLPLIVYFHGGGFCVGSAAWSCYHE 121
             PE  V + DVV+D    +  R Y+P       +KLP++VYFHGGGF + +     YH 
Sbjct: 123 --PE--VATKDVVIDPATGVSVRLYLPNVVDLPSKKLPVLVYFHGGGFVIENTGSPNYHN 182

Query: 122 FLAKLSAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFT 181
           +L  L+AKA  +I+S+NYRLAPE PLPA Y+D +    W+   +     + W +++ DF+
Sbjct: 183 YLTLLAAKAGVLIVSINYRLAPEYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFS 242

Query: 182 KIYLSGDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFL 241
           +I LSGDSAGGN+   VA R                VI+G  ++ P+F G     +E   
Sbjct: 243 QILLSGDSAGGNVTHYVAMRADAG------------VIEGVAIVHPYFLGSEPVGNE--- 302

Query: 242 VQPPRSPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDIL 301
                 P  +   D  WRLA P     D P  NP   G  ++  L+   +++ ++  D L
Sbjct: 303 ---INDPANIEFHDKLWRLAAPDTEGLDDPLINPVAPGAPSLAGLKCKRAVVFVAGNDFL 362

Query: 302 KDRNLEFCSALHRAG--KLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFL 350
            +R   +  AL ++G       V +EGVGH F +   S  S   ++ M+  + AFL
Sbjct: 363 VERGRMYYEALVKSGWRGEAELVQHEGVGHVFHL---SDYSGDISVAMMTKLIAFL 382

BLAST of Cucsat.G9435 vs. NCBI nr
Match: XP_004135407.1 (probable carboxylesterase 6 [Cucumis sativus] >XP_011655765.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741302.1 probable carboxylesterase 6 [Cucumis sativus] >XP_031741303.1 probable carboxylesterase 6 [Cucumis sativus] >KGN52062.1 hypothetical protein Csa_008345 [Cucumis sativus])

HSP 1 Score: 728 bits (1878), Expect = 3.99e-265
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS
Sbjct: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351

BLAST of Cucsat.G9435 vs. NCBI nr
Match: KAA0034538.1 (putative carboxylesterase 6 [Cucumis melo var. makuwa] >TYK09092.1 putative carboxylesterase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 714 bits (1842), Expect = 1.23e-259
Identity = 343/351 (97.72%), Postives = 347/351 (98.86%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTK+MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKKMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAK++CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS
Sbjct: 121 SAKSDCIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRT SEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPR 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVED RVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIK FLCV
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 351

BLAST of Cucsat.G9435 vs. NCBI nr
Match: XP_038893358.1 (probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893360.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893361.1 probable carboxylesterase 6 [Benincasa hispida])

HSP 1 Score: 710 bits (1833), Expect = 2.90e-258
Identity = 342/351 (97.44%), Postives = 345/351 (98.29%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVV+DKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVMDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVG KQNWWSRYCDFTKIYLS
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAV+LKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVLKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLTL VSDTYWRLALPSGTNRDHPWCNPS KGLF VEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLAVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIK FLCV
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCV 351

BLAST of Cucsat.G9435 vs. NCBI nr
Match: XP_008446570.1 (PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446572.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446573.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo] >XP_008446575.1 PREDICTED: probable carboxylesterase 6 [Cucumis melo])

HSP 1 Score: 705 bits (1820), Expect = 2.30e-256
Identity = 339/346 (97.98%), Postives = 342/346 (98.84%), Query Frame = 0

Query: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65
           MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAN 125
           TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAK++
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185
           CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245
           GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRT SEKFLVQPPRSPLTL
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSA 305
           GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVED RVLPSLICISEMDILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIK FLCV
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 346

BLAST of Cucsat.G9435 vs. NCBI nr
Match: XP_022982294.1 (probable carboxylesterase 6 [Cucurbita maxima] >XP_022982375.1 probable carboxylesterase 6 [Cucurbita maxima] >XP_022982445.1 probable carboxylesterase 6 [Cucurbita maxima])

HSP 1 Score: 668 bits (1723), Expect = 1.63e-241
Identity = 323/351 (92.02%), Postives = 332/351 (94.59%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA+WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF G KQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRTKSEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPH 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLT  VSDTYWRLALPS  NRDHPWCNPS  G+F VEDLRVLPSLICISEMDILKDR+L
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIK FL V
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cucsat.G9435 vs. ExPASy TrEMBL
Match: A0A0A0KUG5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608500 PE=4 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 1.93e-265
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS
Sbjct: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351

BLAST of Cucsat.G9435 vs. ExPASy TrEMBL
Match: A0A5A7SV76 (Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00820 PE=4 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 5.95e-260
Identity = 343/351 (97.72%), Postives = 347/351 (98.86%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MRTK+MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP
Sbjct: 1   MRTKKMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAK++CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS
Sbjct: 121 SAKSDCIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRT SEKFLVQPPR
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPR 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVED RVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIK FLCV
Sbjct: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 351

BLAST of Cucsat.G9435 vs. ExPASy TrEMBL
Match: A0A1S3BFC8 (probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1)

HSP 1 Score: 705 bits (1820), Expect = 1.11e-256
Identity = 339/346 (97.98%), Postives = 342/346 (98.84%), Query Frame = 0

Query: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65
           MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAN 125
           TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAK++
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185
           CII+SVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245
           GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRT SEKFLVQPPRSPLTL
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSA 305
           GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVED RVLPSLICISEMDILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM DHIK FLCV
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 346

BLAST of Cucsat.G9435 vs. ExPASy TrEMBL
Match: A0A6J1J448 (probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111481140 PE=4 SV=1)

HSP 1 Score: 668 bits (1723), Expect = 7.91e-242
Identity = 323/351 (92.02%), Postives = 332/351 (94.59%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA+WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSASWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF G KQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRTKSEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTKSEKFLVQPPH 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLT  VSDTYWRLALPS  NRDHPWCNPS  G+F VEDLRVLPSLICISEMDILKDR+L
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRSL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIK FL V
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350

BLAST of Cucsat.G9435 vs. ExPASy TrEMBL
Match: A0A6J1H254 (probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111458883 PE=4 SV=1)

HSP 1 Score: 667 bits (1721), Expect = 1.60e-241
Identity = 323/351 (92.02%), Postives = 330/351 (94.02%), Query Frame = 0

Query: 1   MRTKRMPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALP 60
           MR KRMP INLDQIPSFEEH NGFDQH VLVEEIEGLIRVYGNGYVERPQIVP VSNALP
Sbjct: 1   MRRKRMP-INLDQIPSFEEHGNGFDQHSVLVEEIEGLIRVYGNGYVERPQIVPSVSNALP 60

Query: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKL 120
           PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPL+VYFHGGGFCVGSA WSCYHEFLAKL
Sbjct: 61  PELGVTSWDVVVDKLNNIWARFYIPTQCQEKLPLLVYFHGGGFCVGSATWSCYHEFLAKL 120

Query: 121 SAKANCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLS 180
           SAKA+CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAF G KQNWWSRYCDFTKIYL 
Sbjct: 121 SAKADCIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFNGAKQNWWSRYCDFTKIYLG 180

Query: 181 GDSAGGNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPR 240
           GDSAGGNIAFNVAARLGGKTTASGAV++KPLVIKG+ILIQPFFGGESRT SEKFLVQPP 
Sbjct: 181 GDSAGGNIAFNVAARLGGKTTASGAVVMKPLVIKGNILIQPFFGGESRTMSEKFLVQPPH 240

Query: 241 SPLTLGVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNL 300
           SPLT  VSDTYWRLALPS  NRDHPWCNPS  G+F VEDLRVLPSLICISEMDILKDRNL
Sbjct: 241 SPLTTAVSDTYWRLALPSRANRDHPWCNPSANGVFEVEDLRVLPSLICISEMDILKDRNL 300

Query: 301 EFCSALHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351
           EFCSALHR GKLINYVVYEGVGHAFQVLNKSQLSQTRTLEM+DHIK FL V
Sbjct: 301 EFCSALHRVGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMMDHIKDFLSV 350