Cucsat.G9219 (gene) Cucumber (B10) v3

Overview
NameCucsat.G9219
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGlycosyltransferase
Locationctg1658: 1733930 .. 1736779 (+)
RNA-Seq ExpressionCucsat.G9219
SyntenyCucsat.G9219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCGAGTCGTACATGCACCGTCTGAAGATCTCCATTTGCTTCCATTTAACAAACCTCGTAACGCAATACCAAAACTCAAATTCTCCATTTTCCTTCGTGGTATACGACTCTCTCATGCCTTGGGTTTTGGATCTTGCTAGAGCATTCGGGCTTCGTGGTGCTCCTTTCTTCACTCAGTCTTGCGCTGTTATTGCCATTTTTTACCACATCATTCATGGTTCCTTTAAGATTATTCCTCCTGTTGCTGATCAGACAACATGTGTGTCATCGTTGTTGCCTGGATTGCCACTTGATCTCCATGCTTCTGACCTTCCTTCTCTTTTATTACCTGATAACAATAATCCCCAACAGAATAATAATCCATTTTTTCTCAAGTTGATGATTGACCAATTACATGACCTCCCTGAATTGATGTTCGTCAACTCCTTCCATGCCTTGGAAACACAAGTAATTCTTCTAACTCTCTTCATCATTGCTACCTATCATTTTTCTTATTTTTTTGTACAATTCTGCATCACTGTTGTATGAAATTAATTCCTTGTTAAAAATATGCTATATGTGTATTTCCTTTTTGGGTGTTGTTTGTAGTTTTATTCGTTTTTATGTTTTCAAAATTAGATAAAACGTTTATGTGGAATGATTGTTTTTCTTTTTTTTTTTTCTTTTCTTGACAAAGTTTATGGAATGAAATTTTTGCTGTCCGTAAACAAATAAGATAAGGATAGCTATCTGTTTTTTCTTTTCCTCTCTCTTATGATTAACAATTTTGGTTCGTCTTTTATCTTTTAAAGGAATAAAAATTGTTTGGACTTTTGTTTCTCTCTCTCTTATGATTTAACAAGTTTGGCCAGTTGGTAGATTAAAAAAAAAACTATGATGCATGTATGAATCATAGTTTTTTATCTCATCATAATAATAAAGTGTTCAAAAGAAAAAAATGTTTGTTGACAAAATTATATATTTAGTAGGAATTTTTGTGTTTGAATGAAAAGAAAAATTAAATTATTTTAAGATTTAATATAAATAGGCAATGGAGTCTACTGCATGCCAAACTATGAGCTTTGGAACATAATATATCAGGTAGACCCTATCATGAATTTCAAAACATGTATTTATTATAACTTATAAACACCTATCTACGGATATGTATACACAATACTTCAAATTTTCACTTATATCTAAATATTTTAATTTACTTGTAACATGAACAACGTGAGTACATATAAAAGTAGATATGCAGAACTATACAGAACATGTGGCAGTCGTCACGTAGTTCCATTCGGTTAGAACTCAAAAACTGCTACCTCGCAGGAAAAATAAAGGAAGAAAGGTTAAGCTAACAAGCCGAGTAAGCGGTGGTTAAGTTTTTAAAAAAATACTTTAATATGTAAAAGTATAAAATCCATGAAACAATGATGAAAAACCAGAAACAATCGAGATAAAGTATGTGCAGTTTAATATCAAACGTTATATAGTTTATTAGTTTCCATAAATTAGTAGTAGAGAGATAAACATAAACATGAATAACAAATTTATCAGGAAAATTAGTACCGAACATGGTCATCCCATATATAAAATATTTAACATATATAGCAGAGAAACATTGGTCATATATACTATACAGCGAGTATGCTCAAATGCATGGCAAAGAAACATGGTCATATACATGCACGCTGAGCAGCTTTCCAAAGGCAAAACACTCCAAAGCAAAGTCCAAAGCCTATGTGCGTGCCGAAGTGTGTCCCAAAATCTCATGGCTACAATATGGAAACACATTCACCGTAAGTCTAGTAGGTAATCATATCCAATCAAAACATGATTTCATAATAACCTATCAAACTTTGATTTAGAATCATGAAAATTCCAATACAATTATAGGATAATTAACAAAACACAAAACCATATATTTTATATTTGCTTGAAATTATACATAATGATAATTTTTCAGCAGAAAAAAAAAACTCCATGATTGAACTTCTAGAGCTTAAAATTTGGACTGTAAGCTGCTCTAAAATCAGGATAGTAAGTTGTTCTGATTTCGGTTTTCCCTGTAATTAAATTAATTGGGTTACCGTTTGATGTTAATCAAATTTAACCCATTTTAACTAACAAAATTAGTAAAATCCACTTCTCCCCTCTCCCTTTCTTCTTCCCAACCTTACTATTCTTTTTATTTTATTCTGAAACTAAATAATGTTTTCTTTTAAAAATCAGAAGTCAAGCTGATAATTTTTCCTAAACTTTAATTTGTTTAAAAAATTCTAACAAAACATATAAATTCTTTCTAAACAAAACTTTCCTACGTATATATAATTCCTTTTAGATAATTCTCCTTTTCACTTTCTTATAGTAAACATTCTTCAACCAGTACCAACTAAACCATGTTCATTATTTATAAGTTCTAAAAGAATCATTTCCATCATCAACTCCCAATAAAAAACTTAAATGTGGAAATTGCGTTCACAACCTCAAGTTTCAAAAAAACAAATTAAAACGGTTCGTACGATTATCAAATGAAAGCCTAAAAACTATTTTTTTTACAAACTTTTAATTAATCAAATATGATAGATGCAGTTAAGTATTAGTTTAATTATTTAACAAGTTATCGTAACATGAATTCAGGTAATAGAATATCTACAAAGCCAGATGCCACTGAAGACGGTTGGACCAACAGTTCCATCCATACTCATAAACAAGGAGTTGATGGACGACGACCATGATTACGGAATGAATCTAATCAATTCAACTGAAGATGACAACAAGAAAATCATGGGGTGGTTAAACTCTAAAGCTCGCAATTCAGTCATTTACGTGTCATTAGGAACCAGAATATCAAACCTTGGAGAGGA

Coding sequence (CDS)

ATCGAGTCGTACATGCACCGTCTGAAGATCTCCATTTGCTTCCATTTAACAAACCTCGTAACGCAATACCAAAACTCAAATTCTCCATTTTCCTTCGTGGTATACGACTCTCTCATGCCTTGGGTTTTGGATCTTGCTAGAGCATTCGGGCTTCGTGGTGCTCCTTTCTTCACTCAGTCTTGCGCTGTTATTGCCATTTTTTACCACATCATTCATGGTTCCTTTAAGATTATTCCTCCTGTTGCTGATCAGACAACATGTGTGTCATCGTTGTTGCCTGGATTGCCACTTGATCTCCATGCTTCTGACCTTCCTTCTCTTTTATTACCTGATAACAATAATCCCCAACAGAATAATAATCCATTTTTTCTCAAGTTGATGATTGACCAATTACATGACCTCCCTGAATTGATGTTCGTCAACTCCTTCCATGCCTTGGAAACACAAGTAATAGAATATCTACAAAGCCAGATGCCACTGAAGACGGTTGGACCAACAGTTCCATCCATACTCATAAACAAGGAGTTGATGGACGACGACCATGATTACGGAATGAATCTAATCAATTCAACTGAAGATGACAACAAGAAAATCATGGGGTGGTTAAACTCTAAAGCTCGCAATTCAGTCATTTACGTGTCATTAGGAACCAGAATATCAAACCTTGGAGAG

Protein sequence

IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQSCAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNNPFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDDHDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE
Homology
BLAST of Cucsat.G9219 vs. ExPASy Swiss-Prot
Match: Q9SYK9 (UDP-glycosyltransferase 74E2 OS=Arabidopsis thaliana OX=3702 GN=UGT74E2 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-33
Identity = 85/221 (38.46%), Postives = 133/221 (60.18%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           ++ YM R++ SI   L  LV   + S +P   +VYDS MPW+LD+A ++GL GA FFTQ 
Sbjct: 74  LDDYMERVETSIKNTLPKLVEDMKLSGNPPRAIVYDSTMPWLLDVAHSYGLSGAVFFTQP 133

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
             V AI+YH+  GSF +       +T  S   P  P+ L A+DLPS L       + ++ 
Sbjct: 134 WLVTAIYYHVFKGSFSVPSTKYGHSTLAS--FPSFPM-LTANDLPSFLC------ESSSY 193

Query: 121 PFFLKLMIDQLH--DLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMD 180
           P  L++++DQL   D  +++  N+F  LE ++++++QS  P+  +GPTVPS+ ++K L  
Sbjct: 194 PNILRIVVDQLSNIDRVDIVLCNTFDKLEEKLLKWVQSLWPVLNIGPTVPSMYLDKRL-S 253

Query: 181 DDHDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRI 220
           +D +YG +L N+      + M WLNSK  NSV+Y+S G+ +
Sbjct: 254 EDKNYGFSLFNAKV---AECMEWLNSKEPNSVVYLSFGSLV 281

BLAST of Cucsat.G9219 vs. ExPASy Swiss-Prot
Match: P0C7P7 (UDP-glycosyltransferase 74E1 OS=Arabidopsis thaliana OX=3702 GN=UGT74E1 PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.5e-33
Identity = 84/221 (38.01%), Postives = 134/221 (60.63%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           ++ YM R++ SI   L  L+   + S +P   +VYDS MPW+LD+A ++GL GA FFTQ 
Sbjct: 74  LDEYMERVESSIKNRLPKLIEDMKLSGNPPRALVYDSTMPWLLDVAHSYGLSGAVFFTQP 133

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
             V AI+YH+  GSF +       +T  S   P LP+ L+A+DLPS L       + ++ 
Sbjct: 134 WLVSAIYYHVFKGSFSVPSTKYGHSTLAS--FPSLPI-LNANDLPSFLC------ESSSY 193

Query: 121 PFFLKLMIDQLH--DLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMD 180
           P+ L+ +IDQL   D  +++  N+F  LE +++++++S  P+  +GPTVPS+ ++K L  
Sbjct: 194 PYILRTVIDQLSNIDRVDIVLCNTFDKLEEKLLKWIKSVWPVLNIGPTVPSMYLDKRLA- 253

Query: 181 DDHDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRI 220
           +D +YG +L  +      + M WLNSK  +SV+YVS G+ +
Sbjct: 254 EDKNYGFSLFGA---KIAECMEWLNSKQPSSVVYVSFGSLV 281

BLAST of Cucsat.G9219 vs. ExPASy Swiss-Prot
Match: K7NBW3 (Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.6e-30
Identity = 83/223 (37.22%), Postives = 137/223 (61.43%), Query Frame = 0

Query: 5   MHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQSCAVI 64
           + R +  +  +L + + +   S++P  F++YDS MPWVL++A+ FGL  APF+TQSCA+ 
Sbjct: 80  LDRFRQKMTKNLEDFLQKAMVSSNPPKFILYDSTMPWVLEVAKEFGLDRAPFYTQSCALN 139

Query: 65  AIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNNPFFL 124
           +I YH++HG  K+ P    +T  +S  LP +PL L  SDLP+     + +P   +    +
Sbjct: 140 SINYHVLHGQLKLPP----ETPTIS--LPSMPL-LRPSDLPAY----DFDPASTDT--II 199

Query: 125 KLMIDQLHDLPE--LMFVNSFHALETQVIEYLQS-QMPLKTVGPTVPSILINKELMDDDH 184
            L+  Q  ++ +  L+F N+F  LE ++I+++++   P+KTVGPTVPS  ++K + +D H
Sbjct: 200 DLLTSQYSNIQDANLLFCNTFDKLEGEIIQWMETLGRPVKTVGPTVPSAYLDKRVENDKH 259

Query: 185 DYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 225
            YG++L    ED     + WL+SK   SV+YVS G+ +  +GE
Sbjct: 260 -YGLSLFKPNED---VCLKWLDSKPSGSVLYVSYGSLV-EMGE 284

BLAST of Cucsat.G9219 vs. ExPASy Swiss-Prot
Match: W8JMV4 (UDP glycosyltransferase 9 OS=Catharanthus roseus OX=4058 GN=UGT9 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-27
Identity = 74/222 (33.33%), Postives = 127/222 (57.21%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           +E Y  + K S   + T L+ + +  N P   ++YDS MPW+L++A   GL GA FFTQ 
Sbjct: 88  MELYFKQFKASAIENTTKLIQKLKTKNPPPKVLIYDSSMPWILEVAHEQGLLGASFFTQP 147

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
           C+V AI+YH++ G+ K+  P+ +    + S LP LPL L   DLP +         ++N+
Sbjct: 148 CSVSAIYYHMLQGTIKL--PLENSENGMVS-LPYLPL-LEKKDLPGV------QQFEDNS 207

Query: 121 PFFLKLMIDQLHDLPELMFV--NSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMD 180
               +L+ DQ  ++ ++ +V  N+F ALE +V+ ++ S+ P+ TVGPT P+ +   +   
Sbjct: 208 EALAELLADQFSNIDDVDYVLFNTFDALEIEVVNWMGSKWPILTVGPTAPTSMFLLDKKQ 267

Query: 181 DDHDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRIS 221
            +++ G ++    E + +  M WL+ +  ++VIYVS G+  S
Sbjct: 268 KNYEDGRSINYLFETNTEVCMKWLDQREIDTVIYVSFGSLAS 299

BLAST of Cucsat.G9219 vs. ExPASy Swiss-Prot
Match: Q6VAA6 (UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 72/222 (32.43%), Postives = 126/222 (56.76%), Query Frame = 0

Query: 2   ESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQSC 61
           ESY+   K      L +L+ + Q+  +    ++YDS+  WVLD+A  FG+ G  FFTQ+C
Sbjct: 83  ESYLETFKQVGSKSLADLIKKLQSEGTTIDAIIYDSMTEWVLDVAIEFGIDGGSFFTQAC 142

Query: 62  AVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNNP 121
            V +++YH+  G   +  P+ +  +     +PG P+ L   + P +L     N +Q  +P
Sbjct: 143 VVNSLYYHVHKGLISL--PLGETVS-----VPGFPV-LQRWETPLIL----QNHEQIQSP 202

Query: 122 FFLKLMIDQLHDLPEL--MFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDD 181
            + +++  Q  ++ +   +F NSF+ LE +VIE+ +    LK +GPT+PS+ ++K L DD
Sbjct: 203 -WSQMLFGQFANIDQARWVFTNSFYKLEEEVIEWTRKIWNLKVIGPTLPSMYLDKRL-DD 262

Query: 182 DHDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISN 222
           D D G NL  +   ++ + M WL+ K + SV+YV+ G+ + +
Sbjct: 263 DKDNGFNLYKA---NHHECMNWLDDKPKESVVYVAFGSLVKH 287

BLAST of Cucsat.G9219 vs. NCBI nr
Match: XP_004137036.1 (UDP-glycosyltransferase 74E2 [Cucumis sativus] >KGN43658.1 hypothetical protein Csa_017206 [Cucumis sativus])

HSP 1 Score: 443 bits (1140), Expect = 6.39e-153
Identity = 220/224 (98.21%), Postives = 220/224 (98.21%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHL NLVTQYQNSN PFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS
Sbjct: 85  IESYMHRLKTSICFHLINLVTQYQNSNFPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 144

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
           CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN
Sbjct: 145 CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 204

Query: 121 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDD 180
           PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLK VGPTVPSILINKELMDDD
Sbjct: 205 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKMVGPTVPSILINKELMDDD 264

Query: 181 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE
Sbjct: 265 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 308

BLAST of Cucsat.G9219 vs. NCBI nr
Match: XP_008455270.1 (PREDICTED: UDP-glycosyltransferase 74E2-like [Cucumis melo])

HSP 1 Score: 386 bits (991), Expect = 2.54e-130
Identity = 200/227 (88.11%), Postives = 210/227 (92.51%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHLT LVTQY+NSNSPF+FVVYDSLM WVLDLARAFGLRGAPFFTQS
Sbjct: 87  IESYMHRLKTSICFHLTKLVTQYRNSNSPFTFVVYDSLMSWVLDLARAFGLRGAPFFTQS 146

Query: 61  CAVIAIFYHIIHGSFKIIPPVAD-QTTCVSSL-LPGLPLDLHASDLPSLLLPDNNNPQQN 120
           CAVIAIFYHIIHGS K IPPVA  +T C  SL LPGLPLDLHASDLPSLLLP+NNNPQQN
Sbjct: 147 CAVIAIFYHIIHGSCKNIPPVAPAETRCELSLTLPGLPLDLHASDLPSLLLPNNNNPQQN 206

Query: 121 NNPFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMD 180
           N PFF KLMIDQLHDLP+LMFVNSFHALE QVIE++QSQMPLKTVGPTVPSILINKELMD
Sbjct: 207 N-PFFRKLMIDQLHDLPDLMFVNSFHALEPQVIEFIQSQMPLKTVGPTVPSILINKELMD 266

Query: 181 DDHDYGMNLINSTE-DDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           DDH YGMNLINST+ DDN KIMGWLNSKAR+SVIYVSLGTR+SNLGE
Sbjct: 267 DDHVYGMNLINSTDQDDNNKIMGWLNSKARHSVIYVSLGTRVSNLGE 312

BLAST of Cucsat.G9219 vs. NCBI nr
Match: XP_038888325.1 (UDP-glycosyltransferase 74E2-like [Benincasa hispida] >XP_038888326.1 UDP-glycosyltransferase 74E2-like [Benincasa hispida] >XP_038888327.1 UDP-glycosyltransferase 74E2-like [Benincasa hispida])

HSP 1 Score: 308 bits (789), Expect = 5.06e-100
Identity = 166/224 (74.11%), Postives = 186/224 (83.04%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHLT+LVTQ Q+SNSPF +VVYDSLMPW+LD+A AFGLRGAPFFTQS
Sbjct: 87  IESYMHRLKTSICFHLTSLVTQQQSSNSPFVYVVYDSLMPWILDVATAFGLRGAPFFTQS 146

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
            AV AIFYHI HGSFK+  PVA+       LLPGLPL LHASDLPSLL+P   NPQ  +N
Sbjct: 147 SAVNAIFYHINHGSFKL--PVAETGV----LLPGLPL-LHASDLPSLLIP---NPQ--HN 206

Query: 121 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDD 180
           PFFLKLMIDQLHDLP+ MF+NSFHALETQ IE++Q  +PLKTVGPT+PSI+I+KEL  DD
Sbjct: 207 PFFLKLMIDQLHDLPDWMFINSFHALETQAIEWMQRHIPLKTVGPTIPSIMIDKELKIDD 266

Query: 181 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           H+Y MNL  STE+DN KIM WL+SK  NSVIYVSLGT  SNL E
Sbjct: 267 HNYRMNLTKSTENDNSKIMEWLDSKVHNSVIYVSLGTT-SNLRE 297

BLAST of Cucsat.G9219 vs. NCBI nr
Match: KAA0031536.1 (UDP-glycosyltransferase 74E2-like [Cucumis melo var. makuwa] >TYK06989.1 UDP-glycosyltransferase 74E2-like [Cucumis melo var. makuwa])

HSP 1 Score: 261 bits (666), Expect = 2.13e-84
Identity = 134/153 (87.58%), Postives = 139/153 (90.85%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHLT LVTQY+NSNSPF+FVVYDSLM WVLDLARAFGLRGAPFFTQS
Sbjct: 87  IESYMHRLKTSICFHLTKLVTQYRNSNSPFTFVVYDSLMSWVLDLARAFGLRGAPFFTQS 146

Query: 61  CAVIAIFYHIIHGSFKIIPPVAD-QTTCVSSL-LPGLPLDLHASDLPSLLLPDNNNPQQN 120
           CAVIAIFYHIIHGS K IPPVA  +T C  SL LPGLPLDLHASDLPSLLLP+NNNPQQN
Sbjct: 147 CAVIAIFYHIIHGSCKNIPPVAPAETRCELSLTLPGLPLDLHASDLPSLLLPNNNNPQQN 206

Query: 121 NNPFFLKLMIDQLHDLPELMFVNSFHALETQVI 151
           N PFF KLMIDQLHDLP+LMFVNSFHALE QVI
Sbjct: 207 N-PFFRKLMIDQLHDLPDLMFVNSFHALEPQVI 238

BLAST of Cucsat.G9219 vs. NCBI nr
Match: XP_023538720.1 (UDP-glycosyltransferase 74E2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234 bits (596), Expect = 4.61e-71
Identity = 131/224 (58.48%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           +E Y HRL+ S CFHL  L+T +Q SN+P + VVYDSL PWVLD+AR FGL GAPFFT+S
Sbjct: 81  VEFYFHRLQTSTCFHLVKLITHHQTSNAPIACVVYDSLTPWVLDVARGFGLPGAPFFTES 140

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
           CAV A+FYHI  GS KI       +   S  LP LPL L  +DLPSL+    +NP Q   
Sbjct: 141 CAVNALFYHIYCGSLKI------PSDKKSVSLPALPL-LQDTDLPSLI----SNPHQY-- 200

Query: 121 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDD 180
           P FL++M +Q  + P+ MF+N+FHALE QV++++QS MPLKTVGPTVPSILI+K LMDD+
Sbjct: 201 PVFLRMMTEQFCNQPDWMFINTFHALEPQVLQWMQSHMPLKTVGPTVPSILIDKGLMDDN 260

Query: 181 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           + YGMNLI STEDD+K I  WL+SK   S+IYVS G+ +S LGE
Sbjct: 261 N-YGMNLIKSTEDDSKTIE-WLDSKDSESIIYVSFGS-VSELGE 288

BLAST of Cucsat.G9219 vs. ExPASy TrEMBL
Match: A0A0A0K2F3 (Glycosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051380 PE=3 SV=1)

HSP 1 Score: 443 bits (1140), Expect = 3.09e-153
Identity = 220/224 (98.21%), Postives = 220/224 (98.21%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHL NLVTQYQNSN PFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS
Sbjct: 85  IESYMHRLKTSICFHLINLVTQYQNSNFPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 144

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
           CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN
Sbjct: 145 CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 204

Query: 121 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDD 180
           PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLK VGPTVPSILINKELMDDD
Sbjct: 205 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKMVGPTVPSILINKELMDDD 264

Query: 181 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE
Sbjct: 265 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 308

BLAST of Cucsat.G9219 vs. ExPASy TrEMBL
Match: A0A1S3C0N5 (Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103495473 PE=3 SV=1)

HSP 1 Score: 386 bits (991), Expect = 1.23e-130
Identity = 200/227 (88.11%), Postives = 210/227 (92.51%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHLT LVTQY+NSNSPF+FVVYDSLM WVLDLARAFGLRGAPFFTQS
Sbjct: 87  IESYMHRLKTSICFHLTKLVTQYRNSNSPFTFVVYDSLMSWVLDLARAFGLRGAPFFTQS 146

Query: 61  CAVIAIFYHIIHGSFKIIPPVAD-QTTCVSSL-LPGLPLDLHASDLPSLLLPDNNNPQQN 120
           CAVIAIFYHIIHGS K IPPVA  +T C  SL LPGLPLDLHASDLPSLLLP+NNNPQQN
Sbjct: 147 CAVIAIFYHIIHGSCKNIPPVAPAETRCELSLTLPGLPLDLHASDLPSLLLPNNNNPQQN 206

Query: 121 NNPFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMD 180
           N PFF KLMIDQLHDLP+LMFVNSFHALE QVIE++QSQMPLKTVGPTVPSILINKELMD
Sbjct: 207 N-PFFRKLMIDQLHDLPDLMFVNSFHALEPQVIEFIQSQMPLKTVGPTVPSILINKELMD 266

Query: 181 DDHDYGMNLINSTE-DDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           DDH YGMNLINST+ DDN KIMGWLNSKAR+SVIYVSLGTR+SNLGE
Sbjct: 267 DDHVYGMNLINSTDQDDNNKIMGWLNSKARHSVIYVSLGTRVSNLGE 312

BLAST of Cucsat.G9219 vs. ExPASy TrEMBL
Match: A0A5A7SND6 (UDP-glycosyltransferase 74E2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003050 PE=4 SV=1)

HSP 1 Score: 261 bits (666), Expect = 1.03e-84
Identity = 134/153 (87.58%), Postives = 139/153 (90.85%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           IESYMHRLK SICFHLT LVTQY+NSNSPF+FVVYDSLM WVLDLARAFGLRGAPFFTQS
Sbjct: 87  IESYMHRLKTSICFHLTKLVTQYRNSNSPFTFVVYDSLMSWVLDLARAFGLRGAPFFTQS 146

Query: 61  CAVIAIFYHIIHGSFKIIPPVAD-QTTCVSSL-LPGLPLDLHASDLPSLLLPDNNNPQQN 120
           CAVIAIFYHIIHGS K IPPVA  +T C  SL LPGLPLDLHASDLPSLLLP+NNNPQQN
Sbjct: 147 CAVIAIFYHIIHGSCKNIPPVAPAETRCELSLTLPGLPLDLHASDLPSLLLPNNNNPQQN 206

Query: 121 NNPFFLKLMIDQLHDLPELMFVNSFHALETQVI 151
           N PFF KLMIDQLHDLP+LMFVNSFHALE QVI
Sbjct: 207 N-PFFRKLMIDQLHDLPDLMFVNSFHALEPQVI 238

BLAST of Cucsat.G9219 vs. ExPASy TrEMBL
Match: A0A6J1GMP0 (Glycosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455839 PE=3 SV=1)

HSP 1 Score: 230 bits (587), Expect = 5.08e-70
Identity = 131/224 (58.48%), Postives = 162/224 (72.32%), Query Frame = 0

Query: 1   IESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQS 60
           +ESY+HRL+IS  FHL  L+T +Q SNSP + VVYDSL PWVLD+AR FGL GAPFFT+S
Sbjct: 82  LESYIHRLQISTSFHLVKLITHHQTSNSPIACVVYDSLTPWVLDVARGFGLPGAPFFTES 141

Query: 61  CAVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNN 120
           CAV A+FYHI  GS KI       +   S  LP LPL L  +DLPSL+    +NP Q   
Sbjct: 142 CAVNAVFYHIYSGSLKI------PSDKKSVSLPALPL-LQDTDLPSLI----SNPHQY-- 201

Query: 121 PFFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDD 180
           P FL++M  Q  + P+ MF+N+FHALE QV++++Q+  PLK VGPTVPSILI+K LMDD+
Sbjct: 202 PVFLRMMTHQFCNQPDWMFINTFHALEPQVLQWMQTHTPLKAVGPTVPSILIDKGLMDDN 261

Query: 181 HDYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLGE 224
           + YGMNLI STEDD+K I  WL+SK   SVIYVS G+ +S LGE
Sbjct: 262 N-YGMNLIKSTEDDSKTIE-WLDSKDSESVIYVSFGS-VSELGE 289

BLAST of Cucsat.G9219 vs. ExPASy TrEMBL
Match: A0A6J1DTN7 (Glycosyltransferase OS=Momordica charantia OX=3673 GN=LOC111024304 PE=3 SV=1)

HSP 1 Score: 196 bits (498), Expect = 1.00e-56
Identity = 113/222 (50.90%), Postives = 160/222 (72.07%), Query Frame = 0

Query: 2   ESYMHRLKISICFHLTNLVTQYQNSNSPFSFVVYDSLMPWVLDLARAFGLRGAPFFTQSC 61
           +S+M RL+ SI FH+T+LV +++ S +P   ++YDS+MPWVLD+A+  G+ GA FFT+SC
Sbjct: 85  DSHMARLRASISFHITDLVARHRTSPAPVRCLIYDSIMPWVLDVAKGLGVFGAXFFTESC 144

Query: 62  AVIAIFYHIIHGSFKIIPPVADQTTCVSSLLPGLPLDLHASDLPSLLLPDNNNPQQNNNP 121
           AV AIFYH+  GSF I  PV D +  ++  LP LP  L  SDLPSL+    ++P + +  
Sbjct: 145 AVNAIFYHLSCGSFTI--PV-DPSFALA--LPALP-PLRVSDLPSLV----SSPDRYSG- 204

Query: 122 FFLKLMIDQLHDLPELMFVNSFHALETQVIEYLQSQMPLKTVGPTVPSILINKELMDDDH 181
            FL  M+DQ  + P+ MF+N+F++LE QVIE++QS   LKTVGPTVPS + +K L +D H
Sbjct: 205 -FLDFMVDQFSNQPDWMFINTFNSLEPQVIEWMQSHTSLKTVGPTVPSTITDKRLTED-H 264

Query: 182 DYGMNLINSTEDDNKKIMGWLNSKARNSVIYVSLGTRISNLG 223
           +YG++L  S+EDD+K IM WL+SK RNSVIY+S G+ ++ LG
Sbjct: 265 EYGISLFKSSEDDSK-IMEWLDSKDRNSVIYMSFGS-VTKLG 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYK91.5e-3338.46UDP-glycosyltransferase 74E2 OS=Arabidopsis thaliana OX=3702 GN=UGT74E2 PE=1 SV=... [more]
P0C7P77.5e-3338.01UDP-glycosyltransferase 74E1 OS=Arabidopsis thaliana OX=3702 GN=UGT74E1 PE=3 SV=... [more]
K7NBW34.6e-3037.22Mogroside IE synthase OS=Siraitia grosvenorii OX=190515 GN=UGT74AC1 PE=1 SV=1[more]
W8JMV43.6e-2733.33UDP glycosyltransferase 9 OS=Catharanthus roseus OX=4058 GN=UGT9 PE=2 SV=1[more]
Q6VAA61.5e-2532.43UDP-glycosyltransferase 74G1 OS=Stevia rebaudiana OX=55670 GN=UGT74G1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004137036.16.39e-15398.21UDP-glycosyltransferase 74E2 [Cucumis sativus] >KGN43658.1 hypothetical protein ... [more]
XP_008455270.12.54e-13088.11PREDICTED: UDP-glycosyltransferase 74E2-like [Cucumis melo][more]
XP_038888325.15.06e-10074.11UDP-glycosyltransferase 74E2-like [Benincasa hispida] >XP_038888326.1 UDP-glycos... [more]
KAA0031536.12.13e-8487.58UDP-glycosyltransferase 74E2-like [Cucumis melo var. makuwa] >TYK06989.1 UDP-gly... [more]
XP_023538720.14.61e-7158.48UDP-glycosyltransferase 74E2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0K2F33.09e-15398.21Glycosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051380 PE=3 SV=1[more]
A0A1S3C0N51.23e-13088.11Glycosyltransferase OS=Cucumis melo OX=3656 GN=LOC103495473 PE=3 SV=1[more]
A0A5A7SND61.03e-8487.58UDP-glycosyltransferase 74E2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1GMP05.08e-7058.48Glycosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455839 PE=3 SV=1[more]
A0A6J1DTN71.00e-5650.90Glycosyltransferase OS=Momordica charantia OX=3673 GN=LOC111024304 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 182..217
e-value: 2.7E-39
score: 137.7
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 2..181
e-value: 2.7E-39
score: 137.7
NoneNo IPR availablePANTHERPTHR11926:SF1330GLYCOSYLTRANSFERASEcoord: 1..223
NoneNo IPR availablePANTHERPTHR11926GLUCOSYL/GLUCURONOSYL TRANSFERASEScoord: 1..223
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 4..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G9219.T1Cucsat.G9219.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901576 organic substance biosynthetic process
molecular_function GO:0080043 quercetin 3-O-glucosyltransferase activity
molecular_function GO:0080044 quercetin 7-O-glucosyltransferase activity