Cucsat.G8985 (gene) Cucumber (B10) v3

Overview
NameCucsat.G8985
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGamma-tubulin complex component
Locationctg1635: 80400 .. 83980 (+)
RNA-Seq ExpressionCucsat.G8985
SyntenyCucsat.G8985
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGTAACATAGAATTAGAAAAAAAAAGAGAGGTGATTTGGCATTTTGATAAAATATTTAGAAGATTAGTACAAATTTCCCATAATTGGATGTGGGCGATTTGTACCATTTTCAAATAAAAATAAAACGTAACATAGAATTAGAAAGTTCAAAAATAGAGACGGAGAAGCCCTTGGAAGCAAACCCCAAAGCAAGAGATGAAATGAAATCCGCGCCCACAAACAAAATCAAAATGACCCATTACACGAAATACACTTTCGATCACATCTTTCAATTATTATTGTTTAAACACATACCTTCTTTCCCTTTTCTTCCTTCTACGCTTACTCTCCCATTTTCTCTCACGCCTTCTTCATTTCTCCCCCTCCAATGGAAGAAGACGATTCCACTAAAGTCCTTGATCTCATTAAGGACCTTGTTCTTCGTCTTCTTTCCCACAATCCCACCTCCAATTCCAGCCCTACTTCCTCTGATTTCCAAAAATCTCTTCGCTATGCAATTCGCATTCTCACTAGCCGTATGACTCCCTCCATTGCTCCTGATGCTGCCGCCATTGCTGAGTCCATCAAAAGGAGACTCGCCACTGAGGGTAAGTCTTCTCAGGCCCTCACTTTCGCTGATCTTTACACTAAGTTTGCATCTAAAACTGGGCCTGGGAGTGTGAATAATAAGTGGGCTGTGCTTTACTTGCTCAAAATTGTGGCCGAAGATCGCAAATGTAGACAGACCCAGTTTGAATCTTCAATGCTTTTGCCTAATTTAGTAGCGAGTGATCCTGTTTTGGGCAAGAAGTCTGGGGCGGCCCCGGAGAAGGAGTGGCAGAAGGGGGTTTTGTTGGTTGCGAAAGACCCTGAGAATCTTCGTGACGTTGCTTTTAAGGAGTTTTCTAATTTGCTGAAGGAGGAAAATGAAGTGACTGAAGAGGTTTTGGTGAGGGATGTGTTGTATGCTTGTCAAGGAATTGATGGGAAGTATGTGAAATTTGACAATAATTCTGATGGGTATGTTCTATCCAATTTAGTTAAGGCTTCCAGAGCTACTAGGACGATGGTTCGCAAGCTTTGTGAAATGGGGTGGCTGTTTAGGAAGGTTAAAGGTTATATATCAGAGAGTATGGAACGTTTTCCAGCTGAAGATATTGGGACTGTTGGACATGCCTTTTGTGCTGCATTGCAGGATGAGCTCTCAGAATACTATAAACTGTTGGCCATTCTCGAAGCCCAGTCTATGAATCCAATACCTTTGGTTTCAGAGGCAGCAAGTTCAGGAAACTATTTGTCTTTGAGGAGATTGGCAGTTTGGTTTGCTGAGCCAATGGCGAAAATGAGGCTGATGGCTGTATTGGTTGACAAGTGTAGAGTCTTGAAGGGCGGGACAATGGCTGGGGCCATCCATTTACACGCTCAACATGGTGACCCTTTGGTGCTTGAATTCATGAGGCGTTTGCTGCGACGTGTATGCTCTCCCCTTTTTGAGATGGTGAGGAGTTGGGTTTTAGAAGGGGAGTTGGAAGACATTTTTTCTGAGTTTTTTGTTGTTGGCCAACAAGTGAAAGCTGAATCTCTCTGGAGGGAAGGTTATAGGCTTCACGCTGGCATGCTTCCATCTTTTATTTCACAATCTCTAGCCCAACGTATTTTGAGGACTGGGAAATCCATTAATTTCCTTCGTGTCTGTTGTGAGGATATGGGATGGGCTGATGCTGCAACAGAAGCAGCAGCAGCTGCTGGGACTACTACTAAAAGGGGAGGTCTTGGATATGGTGAAACAGATGCCCTTGAATCTTTGGTAGACGAAGCTGCAAAAAGAATAGACAAACATTTGTTGGATGTAATGCACAAGCGATATAAGTTCAAAGACCATTGTCTAGCAATTAAGCGCTATTTACTATTAGGACAAGGAGATTTTGTCCAATATTTGATGGATATTGTTGGACCTGAGCTTTCTGAGCCTGCTAATGCTATTAGCTCATTTAAGTTATCTGGTCTCCTGGAAACAGCAATTCGGTCCTCTAATGCTCAGTATGATGATCCAGACATCTTGGATAGATTGAAGGTTAAGATGATGCCTCATGGAACTGGAGATAGGGGTTGGGATGTGTTCTCATTAGAATATGAGGCAAGAGTTCCGCTAGATACTGTATTCACAGAGTCTGTCATGTCAAAATATTTAAGGATTTTTAATTTCCTGTGGAAGCTTAGACGTGTTGAGCATGCACTTATAGGTACTTGGAAGACCATGAAACCTAACTGCATCACGTCCTGTTCATTAACTAAGCTGCACCATGGGGTTAAGTTGCAATTACTGTCAACATTGAGGCGATGCCAGGTCCTTTGGGTTGAGATGAATCATTTTGTTACAAACTTGCAGTACTACATAATGTTTGAAGTTTTGGAGGTATCATGGTCTGACTTTTCAAATGAAATGGAAGCAGCAATGGATCTGGATGATTTACTTGCCGCTCATGAAAAGTATCTTCATTCGATCTTTGAGAAATCTCTTCTTGGAGAACAATCTCAGACACTTTGCAAGTCACTTTTTGTCTTATTTGATATAATTTTGCGATTTCGAAGTCATGCGGACAGGTTGTACGAGGGAATACATGAATTACAATGCAGGTAATATTATATTTTTATTTATTTATTTTAATGTGTTTCTATGTACTATTCCTTTCTTCTTGTAATCAGTATATCTCTTTTTCTCATGTAGAACAATAGAATCATCTTTACCCTCTAGAGACAAGAGTAAAAAATCTCGTACGACAGAAAGATCTTTAGAAACAGCATCATGGATAGCAGATGGCAAGAAGGCTTTAACACAACGCGCTGGTGAATTTCTTCGGAATGTTGAACAAGATCTAGCTGCATTAGCTAAAGAGTATTCTTCATTGCTCGAGGAATTCATTTCTCAGCTGCCTTTGCAACAACACGTTGATTTGAAGTTTCTCCTATTTCGCCTTGACTTCACTGAATTTTACAGCCAATTACGACCTCATGTGTAGAGTGGATGTCAGATGTATATTTCTCATGGCCTGCATTGGTCACACAATTGGTAGAGCTCCTAACAAATTTTTCATGGCAGCCACAGGGATCAACCATTGAAAAGTTCATTGCCTGGCATGTAATTTTAGGTATGTGGTTGCTCATTTGTTTATAGTTTGCTGCAGAGGTTGTTATTACATATATATGCCAATCTGTGCTCGTATATGATACTTTCCAGGGTTTTAACCATTTTTATCATTTATGAATAGAAATGGTGTTTGGTATTATGTTTAGCCCAGTAGTCTACAGTTCACTTTAATGTTGTATTTGTTGTCTCATTTTGGATTATTTGTTAGTGTTTTTGTGGCTTTCAAGTCCGTATCCAAGTTCGTAGTTTTCTTTCTTAAGCTGTGTTATGGGGGGCTCTTAGGCTTGGATCTGAGTGCGTTTTTGCACATTTCAGCATCCGTCAGTCATGTAGTTAATTTTTCTTAATGGTATCTTTGATGCTTTATATAGTTCAACACCTGCAAATGTTTAAATTTGACAAGGGCATGATGATCCTTGTCCCTTCCTTTCTATTGTTTAGAATAAAAAAA

Coding sequence (CDS)

ATGGAAGAAGACGATTCCACTAAAGTCCTTGATCTCATTAAGGACCTTGTTCTTCGTCTTCTTTCCCACAATCCCACCTCCAATTCCAGCCCTACTTCCTCTGATTTCCAAAAATCTCTTCGCTATGCAATTCGCATTCTCACTAGCCGTATGACTCCCTCCATTGCTCCTGATGCTGCCGCCATTGCTGAGTCCATCAAAAGGAGACTCGCCACTGAGGGTAAGTCTTCTCAGGCCCTCACTTTCGCTGATCTTTACACTAAGTTTGCATCTAAAACTGGGCCTGGGAGTGTGAATAATAAGTGGGCTGTGCTTTACTTGCTCAAAATTGTGGCCGAAGATCGCAAATGTAGACAGACCCAGTTTGAATCTTCAATGCTTTTGCCTAATTTAGTAGCGAGTGATCCTGTTTTGGGCAAGAAGTCTGGGGCGGCCCCGGAGAAGGAGTGGCAGAAGGGGGTTTTGTTGGTTGCGAAAGACCCTGAGAATCTTCGTGACGTTGCTTTTAAGGAGTTTTCTAATTTGCTGAAGGAGGAAAATGAAGTGACTGAAGAGGTTTTGGTGAGGGATGTGTTGTATGCTTGTCAAGGAATTGATGGGAAGTATGTGAAATTTGACAATAATTCTGATGGGTATGTTCTATCCAATTTAGTTAAGGCTTCCAGAGCTACTAGGACGATGGTTCGCAAGCTTTGTGAAATGGGGTGGCTGTTTAGGAAGGTTAAAGGTTATATATCAGAGAGTATGGAACGTTTTCCAGCTGAAGATATTGGGACTGTTGGACATGCCTTTTGTGCTGCATTGCAGGATGAGCTCTCAGAATACTATAAACTGTTGGCCATTCTCGAAGCCCAGTCTATGAATCCAATACCTTTGGTTTCAGAGGCAGCAAGTTCAGGAAACTATTTGTCTTTGAGGAGATTGGCAGTTTGGTTTGCTGAGCCAATGGCGAAAATGAGGCTGATGGCTGTATTGGTTGACAAGTGTAGAGTCTTGAAGGGCGGGACAATGGCTGGGGCCATCCATTTACACGCTCAACATGGTGACCCTTTGGTGCTTGAATTCATGAGGCGTTTGCTGCGACGTGTATGCTCTCCCCTTTTTGAGATGGTGAGGAGTTGGGTTTTAGAAGGGGAGTTGGAAGACATTTTTTCTGAGTTTTTTGTTGTTGGCCAACAAGTGAAAGCTGAATCTCTCTGGAGGGAAGGTTATAGGCTTCACGCTGGCATGCTTCCATCTTTTATTTCACAATCTCTAGCCCAACGTATTTTGAGGACTGGGAAATCCATTAATTTCCTTCGTGTCTGTTGTGAGGATATGGGATGGGCTGATGCTGCAACAGAAGCAGCAGCAGCTGCTGGGACTACTACTAAAAGGGGAGGTCTTGGATATGGTGAAACAGATGCCCTTGAATCTTTGGTAGACGAAGCTGCAAAAAGAATAGACAAACATTTGTTGGATGTAATGCACAAGCGATATAAGTTCAAAGACCATTGTCTAGCAATTAAGCGCTATTTACTATTAGGACAAGGAGATTTTGTCCAATATTTGATGGATATTGTTGGACCTGAGCTTTCTGAGCCTGCTAATGCTATTAGCTCATTTAAGTTATCTGGTCTCCTGGAAACAGCAATTCGGTCCTCTAATGCTCAGTATGATGATCCAGACATCTTGGATAGATTGAAGGTTAAGATGATGCCTCATGGAACTGGAGATAGGGGTTGGGATGTGTTCTCATTAGAATATGAGGCAAGAGTTCCGCTAGATACTGTATTCACAGAGTCTGTCATGTCAAAATATTTAAGGATTTTTAATTTCCTGTGGAAGCTTAGACGTGTTGAGCATGCACTTATAGGTACTTGGAAGACCATGAAACCTAACTGCATCACGTCCTGTTCATTAACTAAGCTGCACCATGGGGTTAAGTTGCAATTACTGTCAACATTGAGGCGATGCCAGGTCCTTTGGGTTGAGATGAATCATTTTGTTACAAACTTGCAGTACTACATAATGTTTGAAGTTTTGGAGGTATCATGGTCTGACTTTTCAAATGAAATGGAAGCAGCAATGGATCTGGATGATTTACTTGCCGCTCATGAAAAGTATCTTCATTCGATCTTTGAGAAATCTCTTCTTGGAGAACAATCTCAGACACTTTGCAAGTCACTTTTTGTCTTATTTGATATAATTTTGCGATTTCGAAGTCATGCGGACAGGTTGTACGAGGGAATACATGAATTACAATGCAGAACAATAGAATCATCTTTACCCTCTAGAGACAAGAGTAAAAAATCTCGTACGACAGAAAGATCTTTAGAAACAGCATCATGGATAGCAGATGGCAAGAAGGCTTTAACACAACGCGCTGGTGAATTTCTTCGGAATGTTGAACAAGATCTAGCTGCATTAGCTAAAGAGTATTCTTCATTGCTCGAGGAATTCATTTCTCAGCTGCCTTTGCAACAACACGTTGATTTGAAGTTTCTCCTATTTCGCCTTGACTTCACTGAATTTTACAGCCAATTACGACCTCATGTGTAG

Protein sequence

MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQTQFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSGNYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYSQLRPHV
Homology
BLAST of Cucsat.G8985 vs. ExPASy Swiss-Prot
Match: Q9FG37 (Gamma-tubulin complex component 3 OS=Arabidopsis thaliana OX=3702 GN=GCP3 PE=1 SV=1)

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 648/844 (76.78%), Postives = 731/844 (86.61%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNP-TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDA 60
           ME+DD  K  DL+++LVLRL+S NP T N  P S  F K+LRYA RIL+SR+TPS+ PDA
Sbjct: 1   MEDDDQQKAADLVQELVLRLVSQNPQTPNLDPNSPAFLKTLRYAFRILSSRLTPSVLPDA 60

Query: 61  AAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ 120
            AIAES+KRRLAT+GKSS AL FADLYTKFASKTGPGSVNNKWA++YLLKIV++DRK   
Sbjct: 61  TAIAESLKRRLATQGKSSDALAFADLYTKFASKTGPGSVNNKWALVYLLKIVSDDRKSAI 120

Query: 121 TQFESSMLLPNLVASDPVLGKKS-GAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKE 180
              +SS+LLPNL   D   G  S G A +K+W  GVLLV+KDPENLRD+AF+E++ L+KE
Sbjct: 121 NGLDSSVLLPNLGIGDTGNGVLSRGEAKKKDWSNGVLLVSKDPENLRDIAFREYAILVKE 180

Query: 181 ENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLF 240
           ENEVTEEVLVRDVLYA QGIDGKYVKF++  DGY +   VK  RATR MVR L E+GWLF
Sbjct: 181 ENEVTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLF 240

Query: 241 RKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAAS 300
           RKVK +I+ESM+RFPAED+GTVG AFCAALQDELS+YYKLLA+LEAQ+MNPIPLVSE+AS
Sbjct: 241 RKVKTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESAS 300

Query: 301 SGNYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S NYLSLRRL+VWFAEPM KMRLMAVLVDKC+VL+GG MAGAIHLHAQHGDPLV +FM  
Sbjct: 301 SNNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDFMMS 360

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQS 420
           LLR VCSPLFEMVRSWVLEGELED F EFFVVGQ VK + LWREGY+LH  MLPSFIS S
Sbjct: 361 LLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVGQPVKVDLLWREGYKLHPAMLPSFISPS 420

Query: 421 LAQRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAA 480
           LAQRILRTGKSINFLRVCC+D GWADAA+EAAAA+GTTT+RGGLGYGETDALE LV EAA
Sbjct: 421 LAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLGYGETDALEHLVTEAA 480

Query: 481 KRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLS 540
           KRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN ISSF+L+
Sbjct: 481 KRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELA 540

Query: 541 GLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 600
           G LE AIR+SNAQYDD D+LDRL+VKMMPHG+GDRGWDVFSLEYEARVPLDTVFTESV+S
Sbjct: 541 GFLEAAIRASNAQYDDRDMLDRLRVKMMPHGSGDRGWDVFSLEYEARVPLDTVFTESVLS 600

Query: 601 KYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVE 660
           KYLR+FNFLWKL+RVEHALIG WKTMKPNCITS S  KL   VKLQLLS LRRCQVLW E
Sbjct: 601 KYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLWNE 660

Query: 661 MNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQT 720
           MNHFVTN QYYIMFEVLEVSWS+FS EMEAA DLDDLLAAHEKYL++I  KSLLGEQSQT
Sbjct: 661 MNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQSQT 720

Query: 721 LCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWI 780
           + +SLFVLF++ILRFRSHADRLYEGIHELQ R+ ES    R+K+K       S E  SWI
Sbjct: 721 IRESLFVLFELILRFRSHADRLYEGIHELQIRSKES---GREKNK-------SQEPGSWI 780

Query: 781 ADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEF 840
           ++G+K LTQRAGEFL+++ QD+ ++AKEY+S L+ F+S LPLQQ VDLKFL FRLDFTEF
Sbjct: 781 SEGRKGLTQRAGEFLQSMSQDMDSIAKEYTSSLDGFLSLLPLQQSVDLKFLFFRLDFTEF 834

Query: 841 YSQL 843
           YS+L
Sbjct: 841 YSRL 834

BLAST of Cucsat.G8985 vs. ExPASy Swiss-Prot
Match: O73787 (Gamma-tubulin complex component 3 homolog OS=Xenopus laevis OX=8355 GN=tubgcp3 PE=1 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.2e-132
Identity = 297/912 (32.57%), Postives = 457/912 (50.11%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L            +D  +  +YA+R++ S   P++  D   + E IK+   
Sbjct: 12  LLQNLCCRILGKG--------EADVAQQFQYAVRVIGSNFAPTVERDEFLVTEKIKKEFV 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQTQFES-----SM 131
            + + +    F++L+ K  S+   G + N+W++LYLL  ++ED + +  +  S     + 
Sbjct: 72  RQRREADGALFSELHRKLQSQ---GVLKNRWSILYLLLSLSEDPRKQPNKTSSFAALFAQ 131

Query: 132 LLPNLVASDPVLGKKSGAAPEKEWQKGV-------------------------------- 191
            LP    S P    +  + P     + V                                
Sbjct: 132 ALPRDAHSTPYYYARPQSLPLSYQDRNVQCAQNAASIGSSGISSIGMYALNGPTPQSIIQ 191

Query: 192 -----------------------LLVAKDPENLRDVAFKEFSNLLKE------------- 251
                                   L A    + +    K   N +               
Sbjct: 192 GQSNQTPNMGDALRQQLGSRLAWTLAAGQQPSQQSTTTKGLPNTVSRNVPRTRREGDSSG 251

Query: 252 ENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLF 311
             E+TE  LVRD+LY  QGIDGK+VK  N+ + Y +   V  S++ + +  KL E+GWL 
Sbjct: 252 SVEITETSLVRDLLYVFQGIDGKFVKMCNSENCYKVDGKVAVSKSLKDITSKLSELGWLH 311

Query: 312 RKVKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSEA 371
            K+K Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+  
Sbjct: 312 NKIKKYTDQRSLDR----AFGLVGQSFCAALHQELKEYYRLLSVLHSQLQVEDDQGVNLG 371

Query: 372 ASSGNYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFM 431
             S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   +
Sbjct: 372 VESS--LTLRRLLVWTFDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLV 431

Query: 432 RRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQ-VKAESLWREGYRLHAGMLPSFI 491
           + +L  V  P+   +  W+ +GELED + EFFV     VK + LW + Y L   M+PSF+
Sbjct: 432 QHILGLVAYPILNFLYRWIYDGELEDTYHEFFVASDPVVKTDRLWHDKYSLRKSMIPSFM 491

Query: 492 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVD 551
           +   ++++L  GKSINFL   C D   A  A     +A +      L     +A ++ +D
Sbjct: 492 TMDQSRKVLLIGKSINFLHQVCHDQTPASKAMAVGKSAESPKDAAELFTDLENAFQTKID 551

Query: 552 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 611
            A     K+LLDV++K Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA  +   
Sbjct: 552 AAYFDTSKYLLDVLNKNYNLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQH 611

Query: 612 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 671
            L+G+LETA+R++NAQ+D+P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT  
Sbjct: 612 NLTGILETAVRATNAQFDNPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRE 671

Query: 672 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 731
            MS YLR+FNFLW+ +R+E+ L   WK    N        KL  G+  +L   L +C +L
Sbjct: 672 CMSHYLRVFNFLWRAKRMEYILTDIWKGHMCN-------AKLLKGMP-ELSGVLHQCHIL 731

Query: 732 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 791
             EM HF+  +QYYI FEVLE SW +  N++  A DLD ++AAH+ +L +I  + LL  +
Sbjct: 732 ASEMVHFIHQMQYYITFEVLECSWDELWNKVLKAQDLDHIIAAHDVFLDTIISRCLLDSE 791

Query: 792 SQTLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLET 847
           S+ L   L  +FD I+ F++  D LY   + ELQ R         ++ KK R +E     
Sbjct: 792 SRALLNQLRAVFDQIIEFQNAQDALYRAALEELQQRL------QFEERKKERESEGEWGV 851

BLAST of Cucsat.G8985 vs. ExPASy Swiss-Prot
Match: Q96CW5 (Gamma-tubulin complex component 3 OS=Homo sapiens OX=9606 GN=TUBGCP3 PE=1 SV=2)

HSP 1 Score: 467.6 bits (1202), Expect = 2.9e-130
Identity = 303/918 (33.01%), Postives = 461/918 (50.22%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L          + +D  +  +YA+R++ S   P++  D   +AE IK+ L 
Sbjct: 12  LLQNLCCRILGR--------SEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKKELI 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQTQFES-----SM 131
            + + + A  F++L+ K  S+   G + NKW++LYLL  ++ED + + ++  S     + 
Sbjct: 72  RQRREADAALFSELHRKLHSQ---GVLKNKWSILYLLLSLSEDPRRQPSKVSSYATLFAQ 131

Query: 132 LLPNLVASDPV-----------------------------------LGKKSGAAP----- 191
            LP    S P                                    L   SG AP     
Sbjct: 132 ALPRDAHSTPYYYARPQTLPLSYQDRSAQSAQSSGSVGSSGISSIGLCALSGPAPAPQSL 191

Query: 192 ---EKEWQKGV--------------LLVAKDPEN----LRDVAFKEFSNLLKEEN----- 251
              +     GV               L A  P +     + V      N+ +        
Sbjct: 192 LPGQSNQAPGVGDCLRQQLGSRLAWTLTANQPSSQATTSKGVPSAVSRNMTRSRREGDTG 251

Query: 252 ---EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWL 311
              E+TE  LVRD+LY  QGIDGK +K +N  + Y +      SR+ R    +L E+GWL
Sbjct: 252 GTMEITEAALVRDILYVFQGIDGKNIKMNNTENCYKVEGKANLSRSLRDTAVRLSELGWL 311

Query: 312 FRKVKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSE 371
             K++ Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+ 
Sbjct: 312 HNKIRRYTDQRSLDR----SFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNL 371

Query: 372 AASSGNYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEF 431
              S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   
Sbjct: 372 GLESS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSL 431

Query: 432 MRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQ-QVKAESLWREGYRLHAGMLPSF 491
           ++ +L  V  P+   +  W+ +GELED + EFFV     VK + LW + Y L   M+PSF
Sbjct: 432 VQHILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSF 491

Query: 492 ISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLV 551
           ++   ++++L  GKSINFL   C D            +A +      L     +A +  +
Sbjct: 492 MTMDQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKI 551

Query: 552 DEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISS 611
           D A     K+LLDV++K+Y   DH  A++RYLLLGQGDF+++LMD++ PEL  PA  +  
Sbjct: 552 DAAYFETSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQ 611

Query: 612 FKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTE 671
             L+G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT 
Sbjct: 612 HNLTGILETAVRATNAQFDSPEILRRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTR 671

Query: 672 SVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQV 731
             MS YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C +
Sbjct: 672 ECMSHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQCHI 731

Query: 732 LWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGE 791
           L  EM HF+  +QYYI FEVLE SW +  N+++ A DLD ++AAHE +L +I  + LL  
Sbjct: 732 LASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHEVFLDTIISRCLLDS 791

Query: 792 QSQTLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLE 847
            S+ L   L  +FD I+  ++  D +Y   + ELQ R         ++ KK R  E    
Sbjct: 792 DSRALLNQLRAVFDQIIELQNAQDAIYRAALEELQRRL------QFEEKKKQREIE---- 851

BLAST of Cucsat.G8985 vs. ExPASy Swiss-Prot
Match: P58854 (Gamma-tubulin complex component 3 OS=Mus musculus OX=10090 GN=Tubgcp3 PE=1 SV=2)

HSP 1 Score: 457.6 bits (1176), Expect = 3.1e-127
Identity = 294/912 (32.24%), Postives = 453/912 (49.67%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L          + +D  +  ++A+R++ S   P++  D   +AE IK+ L 
Sbjct: 12  LLQNLCCRILGR--------SEADVAQQFQFAVRVIGSNFAPTVERDEFLVAEKIKKELI 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRK--------------- 131
            + + + A  F++L+ K  S+   G + NKW++LYLL  ++ED +               
Sbjct: 72  RQRREADAALFSELHRKLHSQ---GVLKNKWSILYLLLNLSEDPRKQASKVTSYASLFAQ 131

Query: 132 ------------------------CRQTQFESSMLLPNLVASDPVLGKKSGAAPEKEWQK 191
                                    R TQ +SS  L +   S   +   SG  P + +  
Sbjct: 132 ALPRDAHSTPYYYARPQTLPLNYQDRSTQAQSSGSLGSSGISSIGMCGLSGPTPVQPFLP 191

Query: 192 GV---------------------LLVAKDPENLRDVAFKEFSNLLKEE------------ 251
           G                       L    P +    + K F N L               
Sbjct: 192 GQSHQAPGVGDGLRQQLGPRLAWTLTGNQPSSQTPTS-KGFPNALSRNLTRSRREGDPGG 251

Query: 252 -NEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLF 311
             EVTE  LVRD+LY  QGIDGK +K  +  + Y +      +++ R    +L E+GWL 
Sbjct: 252 TLEVTEAALVRDILYVFQGIDGKNIKMSSTENCYKVEAKANLNKSLRDTAVRLAELGWLH 311

Query: 312 RKVKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSEA 371
            K++ Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+  
Sbjct: 312 NKIRKYADQRSLDR----SFGLVGQSFCAALHQELKEYYRLLSVLHSQLQLEDDQGVNLG 371

Query: 372 ASSGNYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFM 431
             S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   +
Sbjct: 372 LESS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMKSLV 431

Query: 432 RRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQ-QVKAESLWREGYRLHAGMLPSFI 491
           + +L  V  P+   +  W+ +GELED + EFFV     VK + LW + Y L   M+PSFI
Sbjct: 432 QHILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFI 491

Query: 492 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVD 551
           +   ++++L  GKSINFL   C D            +A +      L     +A +  +D
Sbjct: 492 TMDQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPRDAADLFTDLENAFQGKID 551

Query: 552 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 611
            A     K+LLDV++K+Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA  +   
Sbjct: 552 AAYFETSKYLLDVLNKKYSLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQH 611

Query: 612 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 671
            L+G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT  
Sbjct: 612 NLTGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRE 671

Query: 672 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 731
            MS YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C +L
Sbjct: 672 CMSHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQCHIL 731

Query: 732 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 791
             EM HF+  +QYYI FEVLE SW +  N ++ A DLD ++AAHE +L++I  + LL   
Sbjct: 732 ASEMVHFIHQMQYYITFEVLECSWDELWNRVQQAQDLDHIIAAHEAFLNTITSRCLLDSN 791

Query: 792 SQTLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLET 847
           S+ L   L  +FD I+  ++  D +Y   + ELQ R         ++ KK R  E     
Sbjct: 792 SRVLLNQLRAVFDQIIELQNAQDVMYRAALEELQRRL------QFEEKKKQREIEGQWGV 851

BLAST of Cucsat.G8985 vs. ExPASy Swiss-Prot
Match: Q95ZG4 (Spindle pole body component 98 OS=Dictyostelium discoideum OX=44689 GN=spc98 PE=1 SV=2)

HSP 1 Score: 358.6 bits (919), Expect = 1.9e-97
Identity = 229/750 (30.53%), Postives = 385/750 (51.33%), Query Frame = 0

Query: 180 NEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVL-----SNLVKA-----SRATRTMVR 239
           NE+ E +L+RD++Y  QGIDG Y+K++  SD + +     + LV       S+  R +V 
Sbjct: 102 NEIPEHLLIRDIIYVFQGIDGTYIKYNKQSDSFKIDENTSNTLVNGEPAYISKPKRDLVY 161

Query: 240 KLCEMGWLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNP 299
           +LCE GWLF+KV+ +I+ +      +  G    +FC+A+ DEL E Y+++AILE Q    
Sbjct: 162 RLCEFGWLFKKVRLFITNN----DFKKTGLTNQSFCSAINDELIELYRIIAILETQVYKK 221

Query: 300 IPLVS-----------------EAASS----------------GNYLSLRRLAVWFAEPM 359
             +V+                 E+ SS                G+ L+L RL VW   P+
Sbjct: 222 FDMVNYGGGGGGSGGSGSGSGLESPSSVSSGGTTTSTEIPFIDGDSLTLIRLFVWIQSPL 281

Query: 360 AKMRLMAVLVDKCRV-LKGGTMAGAIHLHAQHGDPLVLEFMRRLLRRVCSPLFEMVRSWV 419
            +++++   VD   V +KGG +   I   ++HGD  +   +  ++ ++C PLF M+R W+
Sbjct: 282 KRLKVLGTCVDSITVDMKGGEILSKIDTLSKHGDQDIRILIHNIMFKICQPLFSMIRLWM 341

Query: 420 LEGELEDIFSEFFV-VGQQVKAESLWREGYRLHAGMLPSFISQSLAQRILRTGKSINFLR 479
            +GE+ D + EFF+   + V+ E  W+E + + A +LPSFIS  L++RIL  GKSIN+++
Sbjct: 342 FKGEINDPYQEFFIRQYESVQLEKTWKEKFAIVARLLPSFISLPLSKRILIIGKSINYMK 401

Query: 480 VCC-----------------------------------EDMGWADAATEAAAAAGTT--- 539
             C                                   E+ G  D   E       +   
Sbjct: 402 QFCNNFKEDKNDRYYYYNQEDDDDDDEDHDDNDDDDENENQGEDDEIIERKLLIKESKII 461

Query: 540 -TKRGGLGYGETDALESLVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQ 599
             K   L Y   + L+ +++  +++  + LL ++  R+KF +H  A+K+YLLLGQGDF+Q
Sbjct: 462 KEKTKELNYINKEVLQEIIELVSRQSSERLLKIVLNRFKFMNHVKALKKYLLLGQGDFIQ 521

Query: 600 YLMDIVGPELSEPANAISSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGW 659
           YLMD++G +L +P + I   KL G ++TAIR+SNAQ+++ DI++RL + ++P   G+ GW
Sbjct: 522 YLMDLIGEDLLKPTSQIQRHKLVGWMDTAIRNSNAQFEEQDIVNRLDIALLPERPGNIGW 581

Query: 660 DVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLT 719
           D+FSL+Y    PL+T+ + + + +Y +IF+F+W ++RVE++L   W+ ++ +  TS S+ 
Sbjct: 582 DIFSLDYHVDTPLNTILSPNDILRYKKIFHFMWGIKRVEYSLASIWRKIRSS--TSLSIL 641

Query: 720 KLHHGVKLQLLSTLRRCQVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEM-EAAMDLDD 779
               G        + +  ++  EM HF++N QYY+MFEVLE SW +    + + A DLD 
Sbjct: 642 SPIGG-------DIHKSHLIMNEMVHFISNFQYYLMFEVLECSWKNLEKFIDQEATDLDQ 701

Query: 780 LLAAHEKYLHSIFEKSLLGEQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIES 839
           L+ AH +YL  I  K  L   S +  +    L  II++F             LQ + I  
Sbjct: 702 LIEAHHQYLQDICNKMFL-SNSDSCYECFKKLLSIIIKFTL-----------LQTKLINL 761

Query: 840 SLPSRDKSKKSRTTERSLETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEF 842
           S+  +++   + T +  +                  EF  +    L  L +EY++   +F
Sbjct: 762 SIAIQNEKNFNETHQAQVNK----------------EF-NSFRNHLNNLYQEYTTSFYKF 809

BLAST of Cucsat.G8985 vs. NCBI nr
Match: XP_031740197.1 (gamma-tubulin complex component 3 isoform X1 [Cucumis sativus] >XP_031740198.1 gamma-tubulin complex component 3 isoform X2 [Cucumis sativus] >KGN54865.1 hypothetical protein Csa_011935 [Cucumis sativus])

HSP 1 Score: 1657 bits (4291), Expect = 0.0
Identity = 846/846 (100.00%), Postives = 846/846 (100.00%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. NCBI nr
Match: XP_008442226.1 (PREDICTED: gamma-tubulin complex component 3 [Cucumis melo] >KAA0041147.1 gamma-tubulin complex component 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1647 bits (4265), Expect = 0.0
Identity = 839/846 (99.17%), Postives = 845/846 (99.88%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. NCBI nr
Match: TYK02462.1 (gamma-tubulin complex component 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1642 bits (4253), Expect = 0.0
Identity = 836/846 (98.82%), Postives = 843/846 (99.65%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILR GKSINFLRVCCEDMGWAD ATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRAGKSINFLRVCCEDMGWADTATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEY+ARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYDARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. NCBI nr
Match: XP_038881746.1 (gamma-tubulin complex component 3 [Benincasa hispida])

HSP 1 Score: 1628 bits (4215), Expect = 0.0
Identity = 826/846 (97.64%), Postives = 838/846 (99.05%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDS+KVLDLIKDLV RLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSSKVLDLIKDLVFRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIA SIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAGSIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESS+LLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTM+RKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMIRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLA+LEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAVLEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRGGLGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDGAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEA VPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEASVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFD+ILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+R+TE+SLET SW+AD
Sbjct: 721 KSLFVLFDLILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRSTEKSLETTSWVAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLE FISQLP+QQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEGFISQLPMQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QL+PHV
Sbjct: 841 QLQPHV 846

BLAST of Cucsat.G8985 vs. NCBI nr
Match: XP_022928720.1 (gamma-tubulin complex component 3 [Cucurbita moschata])

HSP 1 Score: 1585 bits (4103), Expect = 0.0
Identity = 807/846 (95.39%), Postives = 827/846 (97.75%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDD +KVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESS+LLPNLVASDPVLGKKS  A EKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGA-EKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EV+EEVLVRDVLYACQGIDGKYVKFDNN+DGYVLSN+VKA RATRTMVRKLCE+GWLFRK
Sbjct: 181 EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISE+MERFPAED+GTVGHAFCAALQDELSEYYKLLA++EAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPM KMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLH+GMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRG LGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN+IS FKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFV+NLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ LC
Sbjct: 661 HFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFD+ILRFRS+ADRLYEGIHELQCRTIESSLPSR KS   R+TE+SLETASW+AD
Sbjct: 721 KSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRA EFLRNVEQDLAALAKEYSSLLE FISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QL+P V
Sbjct: 841 QLQPRV 845

BLAST of Cucsat.G8985 vs. ExPASy TrEMBL
Match: A0A0A0KYU4 (Gamma-tubulin complex component OS=Cucumis sativus OX=3659 GN=Csa_4G561690 PE=3 SV=1)

HSP 1 Score: 1657 bits (4291), Expect = 0.0
Identity = 846/846 (100.00%), Postives = 846/846 (100.00%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. ExPASy TrEMBL
Match: A0A5A7THP1 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G00670 PE=3 SV=1)

HSP 1 Score: 1647 bits (4265), Expect = 0.0
Identity = 839/846 (99.17%), Postives = 845/846 (99.88%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. ExPASy TrEMBL
Match: A0A1S3B579 (Gamma-tubulin complex component OS=Cucumis melo OX=3656 GN=LOC103486142 PE=3 SV=1)

HSP 1 Score: 1647 bits (4265), Expect = 0.0
Identity = 839/846 (99.17%), Postives = 845/846 (99.88%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. ExPASy TrEMBL
Match: A0A5D3BUI1 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1738G00840 PE=3 SV=1)

HSP 1 Score: 1642 bits (4253), Expect = 0.0
Identity = 836/846 (98.82%), Postives = 843/846 (99.65%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILR GKSINFLRVCCEDMGWAD ATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRAGKSINFLRVCCEDMGWADTATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEY+ARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYDARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of Cucsat.G8985 vs. ExPASy TrEMBL
Match: A0A6J1ESD9 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111435547 PE=3 SV=1)

HSP 1 Score: 1585 bits (4103), Expect = 0.0
Identity = 807/846 (95.39%), Postives = 827/846 (97.75%), Query Frame = 0

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDD +KVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESS+LLPNLVASDPVLGKKS  A EKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGA-EKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EV+EEVLVRDVLYACQGIDGKYVKFDNN+DGYVLSN+VKA RATRTMVRKLCE+GWLFRK
Sbjct: 181 EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISE+MERFPAED+GTVGHAFCAALQDELSEYYKLLA++EAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPM KMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLH+GMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRG LGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN+IS FKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFV+NLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ LC
Sbjct: 661 HFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFD+ILRFRS+ADRLYEGIHELQCRTIESSLPSR KS   R+TE+SLETASW+AD
Sbjct: 721 KSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRA EFLRNVEQDLAALAKEYSSLLE FISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 846
           QL+P V
Sbjct: 841 QLQPRV 845

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FG370.0e+0076.78Gamma-tubulin complex component 3 OS=Arabidopsis thaliana OX=3702 GN=GCP3 PE=1 S... [more]
O737873.2e-13232.57Gamma-tubulin complex component 3 homolog OS=Xenopus laevis OX=8355 GN=tubgcp3 P... [more]
Q96CW52.9e-13033.01Gamma-tubulin complex component 3 OS=Homo sapiens OX=9606 GN=TUBGCP3 PE=1 SV=2[more]
P588543.1e-12732.24Gamma-tubulin complex component 3 OS=Mus musculus OX=10090 GN=Tubgcp3 PE=1 SV=2[more]
Q95ZG41.9e-9730.53Spindle pole body component 98 OS=Dictyostelium discoideum OX=44689 GN=spc98 PE=... [more]
Match NameE-valueIdentityDescription
XP_031740197.10.0100.00gamma-tubulin complex component 3 isoform X1 [Cucumis sativus] >XP_031740198.1 g... [more]
XP_008442226.10.099.17PREDICTED: gamma-tubulin complex component 3 [Cucumis melo] >KAA0041147.1 gamma-... [more]
TYK02462.10.098.82gamma-tubulin complex component 3 [Cucumis melo var. makuwa][more]
XP_038881746.10.097.64gamma-tubulin complex component 3 [Benincasa hispida][more]
XP_022928720.10.095.39gamma-tubulin complex component 3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KYU40.0100.00Gamma-tubulin complex component OS=Cucumis sativus OX=3659 GN=Csa_4G561690 PE=3 ... [more]
A0A5A7THP10.099.17Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3B5790.099.17Gamma-tubulin complex component OS=Cucumis melo OX=3656 GN=LOC103486142 PE=3 SV=... [more]
A0A5D3BUI10.098.82Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1ESD90.095.39Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111435547 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 265..285
NoneNo IPR availableCOILSCoilCoilcoord: 793..813
NoneNo IPR availablePANTHERPTHR19302:SF61GAMMA-TUBULIN COMPLEX COMPONENTcoord: 28..834
IPR040457Gamma tubulin complex component, C-terminalPFAMPF04130GCP_C_terminalcoord: 497..839
e-value: 7.4E-79
score: 265.4
IPR041470Gamma tubulin complex component protein, N-terminalPFAMPF17681GCP_N_terminalcoord: 188..491
e-value: 1.2E-76
score: 258.3
IPR042241Gamma-tubulin complex, C-terminal domain superfamilyGENE3D1.20.120.1900coord: 496..838
e-value: 3.7E-70
score: 238.5
IPR007259Gamma-tubulin complex component proteinPANTHERPTHR19302GAMMA TUBULIN COMPLEX PROTEINcoord: 28..834

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G8985.T1Cucsat.G8985.T1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0007020 microtubule nucleation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
cellular_component GO:0005815 microtubule organizing center
cellular_component GO:0000922 spindle pole
molecular_function GO:0043015 gamma-tubulin binding