Cucsat.G852 (gene) Cucumber (B10) v3

Overview
NameCucsat.G852
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description(-)-germacrene D synthase-like
Locationctg1: 2957398 .. 2965182 (+)
RNA-Seq ExpressionCucsat.G852
SyntenyCucsat.G852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAGTTAAACTCACAACACAAGCAAAACCCTATCTATCAAAAGAAGCATATAACTATATATTTTACCAATCTTCATGGAAAGAAAAATGTCATTTGAAGAAGTTGAATCAAACTCTATGACATCAAATTCTAATAGCTCCTTCAATGATAATGGTGTTCCTCATCGATCAGAAAAGTTCCAATCAAGTTCTTTGGGACATTATTTCCTTTCTCATCAACCCAAAACTTTGGTATGTCCAATATAAACATCATATGTATATATTTGGTATTTCATGCCTTCGTGATGAAGTGTTTTTGTTTCATATTGTCTATCATTTAAATGAATGTTTTAGTCCAGAATATTTGAGTCTAATTTGGAAGAACTTCATTCTTTCTCTTTAACACACCATGTTAATATCATGCATGTCAATAAATTTTACACTAGACTACTTATTATCTCTAAGACAAACATTATGATAATCCAGGAAGAAGACAAGATGATCAGGAAAAAACTTCAAGAACTAAAGGAAGAAGTAAGAAGCATGTTTGTCACTACTAAGAAATTTTCAAAAAAACTAAGTTTAATTGATTCAATCCAATGTTTAGGCTTGTCTTACCATTTTGATGATGAAATAAATGAAGTTTTAATGTTAATGAAAAATCCTTGCAATGTTGACGACGAACATGAGGATCTTTATATTACGGCTCTTAGATTCCGATTACTGCGACAACAAGGTTTCTTTGTTTCATGTGGTAAGTGATCCTCTCCAAGCAATTTTTACCAATATATTTACTTTTTTCTAGTAGGAACTTTAGATTCAAAATATTAACATATATAACAACATTTAAAAAAATTGTAAATATAGTAAAATCCGTCAAAACCTATTACTGATCAACTGCGTGTGGCAAATATTGGTCTATCTATTTTTACAATTTCTTTAAAAAATATTTATATACTCTTAATTATTATTCTTAAAATTTCTTCCAAACTATAGCAATACTCATTTTTAACTCCTTACCCTTTCCATAAATTCTCTCAATATTTTGTATTTAATCTTAGTTTTTAATAATCCTTGAATTTAGACCATTTCTAAAGTTCAAAAGGCTCAAAAATTGAAATTCCAATTTTTTTGTCTAGAGTTTACAAACGTTTAAAAATGCATTATAGATATCTTGTTAAAAGTGTAATAATTTAGTAAGATTTGGTGGAAAAAACTCAGTTATTAACAACCTTTTGTTGAATTAACTTATAAAAACAGAGATATTCAATAAGTTTACGAATGAAAGGGGAGAATTGAAAGAATCAATAAGAAATGATGAAAATGGTTTATTAAGTTTGTATGAAGCGTCCCATTTGAGAATGAAAGGAGAAAATATATTGGATGAAGCATTAGCTTTCACAACTACTCATCTTAAAGCAATGGCCATGGATTCCAACTCTCCATTCTTTGAGGAAGCAAAATATGCATTGAAGTGGCCAATTTATAAAGCTGTGCAAAGGTTTATGGCTAGGCACTATATCTCTCTTTATAGCAACAATCCATTGAAGAATAATGCTTTGCTCACTTTTGCAAAGTTGGACTACAATTCTCTCCAAAAACTTTACCAGAAAGAACTAAGTGAACTTTCAAGGTGAAACTTCCTATTGATCAGCATGTTCCTCAAATAGTTTAAACTTTGTTGATTTTCACTCGCTTTTAAGACCTGTTTGGGGTAAGGATATTAGAGAAGATGAGTGCTTTTCAGTAAAATTATTTTTATTTAAACTCTTTTAATAAAAATTGAAAAATTATTTTAAAATTCAAAAATACTAAAAATTAACATTCATAAATACAACAAAATATTACCATCTATTTGCAATATAGTATAATATATTGCATTATAATAAAGTGTAATATTTTGTTATATTTATAAATATTTTGGTTCATTTAAACTAGATTTGAAAACAATCTTAAAAATTAATCAAATCAAAATGTTTTGTAAACTATCATATGAAAATGTTTTGTAAACTATTATAAATGGTAGTCTAGATTGATTTTTCTTCAAATTATCTTATTATACTTTTTAATAGACTTATTTTCAAAATTAAAGGGCAGAAAAGTTATACCGATGACTCACCTTAAAAATTCAATAATCTAATCTTAAAGGTAAATATGTAGGTGGTGGAAGAATCAAAAGTTCATGGAGCAATTACATTTTGCAAGAGATAGGGTGGTGGAGTGCTATCTTTGGGTAATAGAAGCATACAATAAACCAAACTATTGTGCCAATAGAAGGATATTTCTCAAAATGGTTAATTTCCTAACTATTATTGATGATATGTATGATACATATGCTACACTTGATGAACTTCAACTTTTCACCGATGCAATTCAAAGGTTTGTTTCTCGTCTCTTTTGTTTGATTTGATTAATGTTTAGATGTGTTTAAAGCTTGCCTAGCTTGCTAGTTTTGTAGTGCACTGAATAAGATGATTCACTTTCAATATCTTCATTTTTAGTTTAGTAGCGATGATTTTGTGCAAAAGAAATTTCAAGTTTAAATAGTTGTAAATTTTATTTATTCTAATCTGGTCATATATACGTGGACCATATGTTGAATATATTGGTGTTAGTCATCCAATTAGTAATTCATTCTCTTGTTTTAAAATCGACAAATAAAGAACACACAAAACAAAAAAAAAAAAGGTAGAAATTGAGAAATTGACTCACTGATTTATTTGTAGTAGACAAAGTAACGGTATGCTAGTAAAGTTTCCTTTCATCTAAAAATAGGGTCGCTTTTAGAGTGGAAATACATGCATGACCAACCATAGATTGTAAGGCCCTGAGTTAAAGAGAGGAACATGAATAAACCGATATCGCAGCTGAATGATAAACATTATTGTAATGCCTTGAGTTAAAGAGAGGAACATGAATAAATCGATATCACATCTGAATGAGAAACATTATAAGAACATGAAAGTAAGATTAAGAAAGACTTACACAATTTTGTCATCATTTTGATATACCATGATTCTTTCATTTCAATTCCTTAACCAAATATAGGACACTGTGTACCCAAATACAAACATGAGTGAAAATTTAATTTGTTATTTATTGCAGTATAATAACTTCAACGTTTTAATTAAAATTCAGGTGGGATATCAAGAGCATTGAGAAGCTCCCAAATTACATGAAAGGTCTTTATGAAGCTATCCTTGAATTTTTTGGAGAGATTGAACAAGATATGAGCATGGATAATAACAATATTCCATTTGCATTGGATTGTGCAAAAGAAGCGGTAAATTCTATTATTGTAGACTTGTAGTCTTTTTGTTTCATATATATTTTTCCTAGAAGAAGATAGGGTTAAAGAACTAGTACACTCACAATTAGCTTTGCTTATAGGCTCTTTTTGAAGGTCTTGCAAGTGAGATTCAGTATTAAATTGAGTAAAAATGGCTTTACAAAGAATTTAAACTTTGTGAGTTATGCTATGTTGATCAATTTAATCTTAGATCCATACTCTTTCTAATGTATGTTTGGAGTTTTGGGTACAGCTAAAAAGACAATGCAAAGCTTATTTGGTGGAAGCAAAATGGTTGAGTGAAGGATATGTGCCAACGATGGAAGAATACATGAATGTTGGCGTTGTTTCAGTTGGCACCTATGTTACGGCTTTAGTTCCCTTCTTAGCTTTGGGAAATGTTGCTTCAAAAGAGGTTTTCCAATGGGTTCAAACTGATCCAATGCTTCTTAAAGCAGGTGGAATAATTGCCCGACTCATGAATGACATAACTTCCCACAAGGTAAAGAATTTAAAATTAGATCAATTTTAATTTTAAGTTCGCTTAATTCTAATCTCTACATTTTCAATAAATATTAAATTTAGTTCTTGATGCGAGAGTGTACGGGAGAGCATTAATTTTTTGAAAAACCTTTTTTCAACTATAACACACTTTTTTTTACTATAAATTTTGAAAATATATTCTCATATTGTTGTATTTATTTGCATGAAAAAAATTAAATTATCATAAGTGTTGGTGGAAATTAATATTGTTTGTGAAATGTAAGATTTATTTAAAGAACACATGAATTTCATATTGATAAAAAAAAAATATTAATTTGGCTACGTCTATCTTGAACTTTGATATCTATTAGATAAATTAAACATGAAATTTCGACACAAAATTATCTTATTTTGGCTTTCACACCTAAACATCTTTTGTGATTATATATATATACAGTGCCATTTTAAAAAATAGTAAAATAAACTAAATATTTACGATATATTGCAATTTTAAATTCTGTCAATAATAAATACCGATAGACTTTTATCACTATCTATCAATATAACTGATAAAAGCATATCGGTCTCTATTATTTGTAGAATCTAAAATTTCGCTATATATTGTAAATATTTTATTAAATTTGTCATTTTTTACAATTTTATATATATATTTAGTTGTTGTCAAATATAGAAAATGTAAGTTTTTAAAACCAGTAGATTTTACAAAATATTTACACTTTATAGCAAATTCTATCATTGATGATCATTATATTCTATAGTGATAGAATACTGTAAGTGATAAAATCCAAAATTTTACTATAGCTTGTAAATATTTTAATTTATTTTGCTATTTTTTAAAAAATGTTTCTATAGAAAAATAAATCAAAATATTTACCAAATTCTAGCAAAATATCAAAGTCTATCTGCGATAGACCCCGATGATTACTGTTTGTGTTATGATCTTAGCATCCTCGATAGCTTTAATCAATCTTTTAAAACACTTCCACCTTTTTCTTCCCTTTCCTATTTTGTCAACTCTCCACCTTAATTTCCTTCTTCATATCTTCATCTTCCTCGTGTTTTGTTTCTTCTTCTTCAGTTGCCCATTTAGATCTAGGTATATATATTATCATAATTTCATGTACCCCATTCCTTATCAATGATCTATCATTCTTTGTTATACTCTTCTCCTTCGTTAAAAAGTCTTTTTCCATCTTTATTCTTGTTAGATTAATTGAAGAGTAATTGTTAGAACACTTCCTAACTTTTATTTTTTTTGCTGGTGTAGTTTCTATTAGTTTCTGATAGACATTGATAGACTTTTACCGGTCTCTATTAGCCGACTTTATTTATCTCAATCACTAATAAAAATTGATAGACTTCTATCAACACTCCTCTATCAATGATAGATTTGTTGTATTAGTTTATATCATTAATAGCGATATTGATAGATCACTTCTATCAGTCTCTATCGATGATAGAATTGTATTGGTTTCTATCAGTTGATAGACTTCTATTAACCTCTATCAATGATATACTTCAACCAATTTCTATCATTAATATCGATAAATACTAATAGACTTTTATCAACTAAATATTAAAGGCTATAAACTTGTATCCATTTCCATTAGTAATAGACATTGGTATAGGCTTTCTCTCAATCTCTTTTAGTGATAGACTTATGTAAGTTTTTATCACTGATAGACACCGATAGATTTCTATTAGTCTCTATCAACAACAAATTGCATGTATCGTTGATAAACACAATGGTAATAAAAGCTAATAGACTTCTATCAATCTCTATTAATTATAGACTCTTATGCTAATCTTATCACTGATAGACTTCTATCAGGCTCTAATGATAAAATTCTATCAGTTTCTATCATTTTAATAGACAGTGATAAATTTCTATCAGTATCTACCAATGATAAATATTGATAAAAAAATTTCAATCTTTATCATTAGAAACTAATGGTGGTCTATCAACGTGTCTATCTGATATAGACATTGATAGAACCCTCTAAACGTCTAACTAAAGCAATTGAAACCATCAAATCAATCCGATAATCAAACTAAACTCAAAACAAAACAAACATCAACACACAAAACCAACATCAAACACCAAACCAACAACTACCCAATGCAATAGCTAGAGCCCGAAGACAAACGCAATCTCATTCATAACAAACATATATCATTATAGATATAGCATTTATGTACATATTACCGAGACTCATGATAGACAATCACTGATAGAAGATTTCAATTGTGATAGACATTGATAGCACACTATGAGTGTTTATTTGAGATTAACATCAATAAAATACTATCTGTCTATCAATAATAGACATGCTCTATCACAAGTATTTTTATACCTTTGGTTGTACTTTTCCGTGTTCCTTTTTAGTTTTATATCTTTTGGTTTTTTTGAATGGTTCTCCCTTTAATTAACATGTTTCCTTTAATTATTGTTTCCATTTCTGTAAACAAAACACAGTTGTGATGTAAAAGACTAAGATTGGAAATTAAATAATACTTCAAACAAAATGTACAAAGAACTTACTTTGAATGCTAGAGAGATAAATAGCTAAAAATATCAAAAGAGAGTGAACAAAAATAAAAATCATTGAAAACAATGCTAAAATTGTATTAAACAACCTAACTCAGTCTTCGTCAATAACAACCATAATCAAGATCGAATAGAGGTTATTCTACGAAAATAACGCAGAAAGAAAATTTTTGAGCTGGAACGTGGTTTTTCTTTGACTAAAAAGTACTAGAAAACAATTAGAAAAAAACATTTCGATTGAATAAACATCTCAAGATGAATAATCAGCAAAAGAAAAAAAAAAAACTTAAAAAAGAGAACAATGTGACAAGAATATTTAGCTCGACCAAACCCAAAACAATTACGAAAGAAGCTGCAGACCACAAAAATTGAAACTAAAGATTAGTATAAAGCAGTTGTGGAAAAAGAAGAAACTTTGGGGTCTGTGGTGGAAAAAAGAAGAAACGACTGGTGGAAAGTAGAAGAGGAAGATTGAGGAGAAAAATAGTATAAATGAAGAAGAACCGTCACTGCGTGCATTGAATGGAAATTTTAGTTTGAAAATGAAAAAAAATCGAAGAGAAAATCGTGATGAATTGTGGATCATGCGGGTAGGGTCGGGTCTTGTGAAGGAAAATTGTGACATATTTTTTTTACGTTTGGGCTGAGCATTTTGGGTAATTTACACGGGATAATTGCAAACTTAGTAATTAAATTCAAAATAATTAAGTATATAGCAACATTTTAAAGAAATTGCAAATATAACAAAATTTGTCAAATTTTATCAATGATAGAGTCCATCACTAATAGATCATGTTGTAAAAGTTGGTCTATCACCGATAGATTATACGAGTCTATCAGCGATAGTTTGGCTATATTTGCAATTTTTTTTAAATGTTGCTATATCCTTAATTACTATTCCTCAAATGGTCATCCATTATAATTACCCATTTCACTGGACTGCTATTTAAATAATTTGTGTATTCTTCTATATTTAAAAATCTCTTTTATTAAACTTATTAAATTTATTAATAAAAGATATTTGTATGTTTTCGTGCTTAATTTTTAGTTTGGACAAGAGAGAGAACATGTGGTTTGTGCAGTTGAGTGCTACATGAAGCAATATGGAGTAAGAGAAGGAGAGGCCATAGTTGAACTAAACAAGGAAGTGGTAGAAGCATGGAAAAGTATGATACAAGATCATATTCACACGAAAGTTTGTACCAAGTTTCCAGATGTGATCCTTCACTTGGGGTTGAATCTTGCTCGAGTAACAAACTTTTATTATAAAGAACGAGATGCTTTCACTTTCGTTGAAGGAGAAACTAAACATCTTATCACCTCTTCGCTCACCATGCCAATTTGATCCTATCTGTAATATAGTTTCTTGTTTTTATGAAATATAAGTGTTGACAAACACATAAATATGATTAAAATAATGTTTTTATTGAAGATAATGTATTATATATGTCCTTTAATTGTTATATGAAGTTTAAGAAAATTATCATGTTTAATATATTTTTTTTGTTCAAGTTTTCAACATACTTGGTTATTATGTCATGAATTCAAATTTTCAAAATACGTATGAAGCTATGTGATTCAAACATGTAAAA

Coding sequence (CDS)

ATGAAAGGAGAAAATATATTGGATGAAGCATTAGCTTTCACAACTACTCATCTTAAAGCAATGGCCATGGATTCCAACTCTCCATTCTTTGAGGAAGCAAAATATGCATTGAAGTGGCCAATTTATAAAGCTGTGCAAAGGTTTATGGCTAGGCACTATATCTCTCTTTATAGCAACAATCCATTGAAGAATAATGCTTTGCTCACTTTTGCAAAGTTGGACTACAATTCTCTCCAAAAACTTTACCAGAAAGAACTAAGTGAACTTTCAAGGTGGTGGAAGAATCAAAAGTTCATGGAGCAATTACATTTTGCAAGAGATAGGGTGGTGGAGTGCTATCTTTGGGTAATAGAAGCATACAATAAACCAAACTATTGTGCCAATAGAAGGATATTTCTCAAAATGGTTAATTTCCTAACTATTATTGATGATATGTATGATACATATGCTACACTTGATGAACTTCAACTTTTCACCGATGCAATTCAAAGGTGGGATATCAAGAGCATTGAGAAGCTCCCAAATTACATGAAAGGTCTTTATGAAGCTATCCTTGAATTTTTTGGAGAGATTGAACAAGATATGAGCATGGATAATAACAATATTCCATTTGCATTGGATTGTGCAAAAGAAGCGCTAAAAAGACAATGCAAAGCTTATTTGGTGGAAGCAAAATGGTTGAGTGAAGGATATGTGCCAACGATGGAAGAATACATGAATGTTGGCGTTGTTTCAGTTGGCACCTATGTTACGGCTTTAGTTCCCTTCTTAGCTTTGGGAAATGTTGCTTCAAAAGAGGTTTTCCAATGGGTTCAAACTGATCCAATGCTTCTTAAAGCAGGTGGAATAATTGCCCGACTCATGAATGACATAACTTCCCACAAGTTTGGACAAGAGAGAGAACATGTGGTTTGTGCAGTTGAGTGCTACATGAAGCAATATGGAGTAAGAGAAGGAGAGGCCATAGTTGAACTAAACAAGGAAGTGGTAGAAGCATGGAAAAGTATGATACAAGATCATATTCACACGAAAGTTTGTACCAAGTTTCCAGATGTGATCCTTCACTTGGGGTTGAATCTTGCTCGAGTAACAAACTTTTATTATAAAGAACGAGATGCTTTCACTTTCGTTGAAGGAGAAACTAAACATCTTATCACCTCTTCGCTCACCATGCCAATTTGA

Protein sequence

MKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEALKRQCKAYLVEAKWLSEGYVPTMEEYMNVGVVSVGTYVTALVPFLALGNVASKEVFQWVQTDPMLLKAGGIIARLMNDITSHKFGQEREHVVCAVECYMKQYGVREGEAIVELNKEVVEAWKSMIQDHIHTKVCTKFPDVILHLGLNLARVTNFYYKERDAFTFVEGETKHLITSSLTMPI
Homology
BLAST of Cucsat.G852 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.8e-93
Identity = 179/364 (49.18%), Postives = 250/364 (68.68%), Query Frame = 0

Query: 28  VPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQELKEEVRSMFVTTKKFS-KKLSLI 87
           V  R   F  S  G +FLS+  +    D  +++ +Q+LKEEVR M +     S +KL LI
Sbjct: 20  VGRRCANFHPSIWGDHFLSYASEFTNTDDHLKQHVQQLKEEVRKMLMAADDDSAQKLLLI 79

Query: 88  DSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNK 147
           D+IQ LG++YHF+ EI+EVL  M +   V  E ED+Y  +LRFRLLRQQG+ VSC++FN 
Sbjct: 80  DAIQRLGVAYHFESEIDEVLKHMFDGSVVSAE-EDVYTASLRFRLLRQQGYHVSCDLFNN 139

Query: 148 FTNERGELKESIRNDENGLLSLYEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFE 207
           F +  G  KES+ +D  G+LSLYEA+H R+ GE+ILDEALAFTTTHL++    S++P  E
Sbjct: 140 FKDNEGNFKESLSSDVRGMLSLYEATHFRVHGEDILDEALAFTTTHLQSATKHSSNPLAE 199

Query: 208 EAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSR 267
           +  +ALK PI K + R  ARHY S+Y  +   N ALL  AKLD+N LQKL+QKELS++S 
Sbjct: 200 QVVHALKQPIRKGLPRLEARHYFSVYQADDSHNKALLKLAKLDFNLLQKLHQKELSDISA 259

Query: 268 WWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYAT 327
           WWK+  F  +L FARDRVVECY W++  Y +P +   RRI  K++   +IIDD+YD Y T
Sbjct: 260 WWKDLDFAHKLPFARDRVVECYFWILGVYFEPQFFFARRILTKVIAMTSIIDDIYDVYGT 319

Query: 328 LDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKE 387
           L+EL+LFT+A++RWDI +I++LP YM+  Y+A+L  + EIE++M+ +  +  + L  AKE
Sbjct: 320 LEELELFTEAVERWDISAIDQLPEYMRVCYQALLYVYSEIEEEMAKEGRS--YRLYYAKE 379

Query: 388 AVNS 391
           A+ +
Sbjct: 380 AMKN 380

BLAST of Cucsat.G852 vs. ExPASy Swiss-Prot
Match: B9SCB6 (Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.7e-84
Identity = 162/372 (43.55%), Postives = 243/372 (65.32%), Query Frame = 0

Query: 21  SSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQELKEEVRSMFVTTKKFS 80
           S+   N   H +  F  +  G  FLS   ++ + +  + ++++ LKEEV+ M  +  + S
Sbjct: 9   SNLKPNTTRHLA-SFHPNIWGDRFLSCAAESTDIEDDMEQQVERLKEEVKKMIASADEPS 68

Query: 81  KKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVS 140
           + L+LID +Q LG+SYHF+ EI E L  + N  +  D+ +DL+  ALRFRLLR+QG  VS
Sbjct: 69  QILNLIDLLQRLGVSYHFEKEIEEALQQVLNMNSDSDKDDDLHSVALRFRLLREQGLNVS 128

Query: 141 CEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDEALAFTTTHLKAMAMDS 200
           C++FNKF +  G   ++++ D  G+LSLYEA+H R+ GE ILD+ALAFTTT+L+++  + 
Sbjct: 129 CDVFNKFRDRNGHFIQTLKTDLQGMLSLYEAAHFRVHGEGILDDALAFTTTYLESIVPNL 188

Query: 201 NSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKE 260
           + P   +    L+ P+ K++ R  ARH+IS+Y  +   N  LLTFAKLD+N LQKL+QKE
Sbjct: 189 SPPLAAQISRTLRQPLRKSLARVEARHFISIYQEDTSHNEVLLTFAKLDFNLLQKLHQKE 248

Query: 261 LSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDM 320
           L  +S WWK+  F+ +L F RDRVVE Y W++  Y +P Y   R+   K++N +++IDD+
Sbjct: 249 LKYISLWWKDLDFVNKLPFTRDRVVEGYFWILGVYFEPQYHRARKFVTKVINVVSVIDDI 308

Query: 321 YDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFA 380
           YD Y TL+EL +FTDAI RWDI  I++LP YMK  Y+A+L  + EIE+ +S    +  + 
Sbjct: 309 YDAYGTLEELVVFTDAINRWDIDCIDQLPEYMKVCYKALLNVYEEIERALSEQGRS--YR 368

Query: 381 LDCAKEAVNSII 393
           L  AKEA+  ++
Sbjct: 369 LHYAKEAMKKLV 377

BLAST of Cucsat.G852 vs. ExPASy Swiss-Prot
Match: O49853 ((+)-delta-cadinene synthase isozyme C2 OS=Gossypium arboreum OX=29729 GN=CAD1-C2 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.1e-82
Identity = 167/357 (46.78%), Postives = 237/357 (66.39%), Query Frame = 0

Query: 13  NSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQELKEEVRSM 72
           + M S+S  S N + +  +++ FQ S  G +FL+   K +  D    K+ Q+LKEEVR M
Sbjct: 6   SQMPSSSPLSSNKDEIRPKAD-FQPSIWGDFFLNCPDKNI--DAGTEKRHQQLKEEVRKM 65

Query: 73  FVTTKKFS-KKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRL 132
            V     S +KL+ IDS+Q LG+SYHF  EI + L  + +  N +D   DLY T+LRFRL
Sbjct: 66  IVAPMANSTQKLAFIDSVQRLGVSYHFTKEIEDELENIYH--NNNDAENDLYTTSLRFRL 125

Query: 133 LRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDEALAFTTT 192
           LR+ G+ VSC++FNKF +E+G  K S+ +D  GLL LY+AS+LR+ GE+ILDEA++FTT 
Sbjct: 126 LREHGYNVSCDVFNKFKDEQGNFKSSVTSDVQGLLELYQASYLRVHGEDILDEAISFTTN 185

Query: 193 HLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYN 252
           HL       + P  EE  +ALK  I + + R  ARHY+S+Y +    N ALL FAK+D+N
Sbjct: 186 HLSLAVSSLDHPLSEEVSHALKQSIRRGLPRVEARHYLSVYQDIESHNKALLEFAKIDFN 245

Query: 253 SLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMV 312
            LQ L++KELSE+ RWWK+  F  +L +ARDRVVE Y W+   Y +P Y   R++  K++
Sbjct: 246 MLQFLHRKELSEICRWWKDLDFQRKLPYARDRVVEGYFWISGVYFEPQYSLGRKMLTKVI 305

Query: 313 NFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQ 369
              +I+DD YD+YAT +EL  +T+AI+RWDIK I++LP YMK  Y+A+L+ + E+EQ
Sbjct: 306 AMASIVDDTYDSYATYEELIPYTNAIERWDIKCIDELPEYMKPSYKALLDVYKEMEQ 357

BLAST of Cucsat.G852 vs. ExPASy Swiss-Prot
Match: P93665 ((+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.9e-82
Identity = 166/357 (46.50%), Postives = 237/357 (66.39%), Query Frame = 0

Query: 13  NSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQELKEEVRSM 72
           + M S+S  S N + +  +++ FQ S  G +FL+   K +  D   +K+ Q+LKEEVR M
Sbjct: 6   SQMPSSSPLSSNKDEMRPKAD-FQPSIWGDFFLNCPDKNI--DAETQKRHQQLKEEVRKM 65

Query: 73  FVTTKKFSK-KLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRL 132
            V     S  KL+ IDS+Q LG+SYHF  EI + L  + +  N +D   DLY T+LRFRL
Sbjct: 66  IVAPMANSTLKLAFIDSVQGLGVSYHFTKEIEDELENIYH--NNNDAENDLYTTSLRFRL 125

Query: 133 LRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDEALAFTTT 192
           LR+ GF VSC++FNKF +E+G  K S+ +D  GLL LY+AS+LR+ GE+ILDEA++FT+ 
Sbjct: 126 LREHGFHVSCDVFNKFKDEQGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTSN 185

Query: 193 HLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYN 252
           HL       + P  EE  +ALK  I + + R  ARHY+S+Y +    N  LL FAK+D+N
Sbjct: 186 HLSLAVASLDHPLSEEVSHALKQSIRRGLPRVEARHYLSVYQDIESHNKVLLEFAKIDFN 245

Query: 253 SLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMV 312
            +Q L++KELSE+SRWWK+  F  +L +ARDRVVE Y W+   Y +P Y   R++  K++
Sbjct: 246 MVQLLHRKELSEISRWWKDLDFQRKLPYARDRVVEGYFWISGVYFEPQYSLGRKMLTKVI 305

Query: 313 NFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQ 369
              +I+DD YD+YAT +EL  +T+AI+RWDIK I++LP YMK  Y+A+L+ + E+EQ
Sbjct: 306 AMASIVDDTYDSYATYEELIPYTNAIERWDIKCIDELPEYMKPSYKALLDVYEEMEQ 357

BLAST of Cucsat.G852 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-81
Identity = 160/340 (47.06%), Postives = 223/340 (65.59%), Query Frame = 0

Query: 52  LEEDKMIRKKLQELKEEVRSMFVT-TKKFSKKLSLIDSIQCLGLSYHFDDEINEVL---- 111
           L+ D+ ++++ ++LKEE+R M +   +    KL+L+DSIQ LG+SYHF+DE++E L    
Sbjct: 46  LKIDEGMKERTEKLKEEIRMMMIAYVENQLIKLNLVDSIQRLGVSYHFEDEVDEFLEHIY 105

Query: 112 ------MLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRN 171
                 +L+ N    +   EDL+ITAL FRLLRQQG+ +SC+IF KF ++ G+ KES+  
Sbjct: 106 VSYNNSLLLSNK---NSNGEDLHITALLFRLLRQQGYRISCDIFLKFMDDNGKFKESLVE 165

Query: 172 DENGLLSLYEASHLRMKGENILDEALAFTTTHLKAMA---MDSNSPFFEEAKYALKWPIY 231
           DE GLLSLYEASH+   GE +L+EAL FTTTHL+       + N  F  E   ALK PI 
Sbjct: 166 DERGLLSLYEASHMMGHGEALLEEALEFTTTHLQTYIHRYSNINPSFASEVSNALKLPIR 225

Query: 232 KAVQRFMARHYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQL 291
           K+V R  AR Y+ +Y  +P  N  LL F+KLD+N LQKL+QKELSE+ RWWK+     + 
Sbjct: 226 KSVPRIKAREYLEIYQQHPSHNETLLEFSKLDFNILQKLHQKELSEICRWWKDLDVPTKF 285

Query: 292 HFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAI 351
            FARDR+VECY W + AY +P Y   R++  K++   +I+DD+YD Y T +ELQ+ T AI
Sbjct: 286 PFARDRIVECYFWTLGAYFEPQYSVGRKMLTKVIAIASILDDIYDAYGTFEELQVLTPAI 345

Query: 352 QRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNI 378
           QRWD   +  LP YMK  Y A+LE + EI +++  D N++
Sbjct: 346 QRWDRSMVHTLPLYMKPFYVAMLELYEEIGKEIDKDQNSL 382

BLAST of Cucsat.G852 vs. NCBI nr
Match: XP_031738277.1 ((-)-germacrene D synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 766 bits (1979), Expect = 3.12e-276
Identity = 384/388 (98.97%), Postives = 385/388 (99.23%), Query Frame = 0

Query: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60
           MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK
Sbjct: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60

Query: 61  KLQELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHE 120
           KLQELKEEVRSMFVTT KFSKKLSLIDSIQ LGLSYHFDDEINEVLMLMKNPCNVDDEHE
Sbjct: 61  KLQELKEEVRSMFVTTNKFSKKLSLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVDDEHE 120

Query: 121 DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGEN 180
           DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIR DENGLLSLYEASHLRMKGEN
Sbjct: 121 DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENGLLSLYEASHLRMKGEN 180

Query: 181 ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN 240
           ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN
Sbjct: 181 ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN 240

Query: 241 ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY 300
           ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY
Sbjct: 241 ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY 300

Query: 301 CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL 360
           CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL
Sbjct: 301 CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL 360

Query: 361 EFFGEIEQDMSMDNNNIPFALDCAKEAV 388
           EFFGEIEQDMSMDNNNIPFALDCAKEA+
Sbjct: 361 EFFGEIEQDMSMDNNNIPFALDCAKEAL 388

BLAST of Cucsat.G852 vs. NCBI nr
Match: XP_008448975.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 671 bits (1730), Expect = 7.47e-240
Identity = 333/389 (85.60%), Postives = 364/389 (93.57%), Query Frame = 0

Query: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60
           MERKMSFEEV+S+SMTSNSNSSFNDNGVP  SEK+Q SSLGHYFLSHQPKTL+EDKMI+K
Sbjct: 1   MERKMSFEEVKSDSMTSNSNSSFNDNGVPRGSEKYQPSSLGHYFLSHQPKTLDEDKMIKK 60

Query: 61  KLQELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLML-MKNPCNVDDEH 120
           +LQELKEEVRSMFVT +KFS+KLSLIDSIQ LGLSYHFDDEINEVLML M+NPCN+D+EH
Sbjct: 61  QLQELKEEVRSMFVTIEKFSEKLSLIDSIQRLGLSYHFDDEINEVLMLLMQNPCNIDEEH 120

Query: 121 EDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGE 180
           EDLYITALRFRLLRQQG F+SCEIFNKFTNERGE KESI  D+ GLLSLYEASHLRMKGE
Sbjct: 121 EDLYITALRFRLLRQQGLFISCEIFNKFTNERGEFKESIGKDKIGLLSLYEASHLRMKGE 180

Query: 181 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKN 240
           NILDEALAFTT++LKAMAMDSNSPF+EEAKYALKWPIYKAV+R MARHYISLYSNNPLKN
Sbjct: 181 NILDEALAFTTSYLKAMAMDSNSPFYEEAKYALKWPIYKAVKRLMARHYISLYSNNPLKN 240

Query: 241 NALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPN 300
           N LLTFAKLDYNSLQKLYQ ELSEL+RWWKNQKFMEQLHFARDRVVECYLW +EA+ +P 
Sbjct: 241 NVLLTFAKLDYNSLQKLYQNELSELTRWWKNQKFMEQLHFARDRVVECYLWALEAFYEPK 300

Query: 301 YCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAI 360
           Y ANRRI LKM+ F+TIIDDMYD YAT+DELQLFT+AIQRWD KSIEKLPNYMKGLY+AI
Sbjct: 301 YSANRRILLKMIYFVTIIDDMYDAYATVDELQLFTEAIQRWDTKSIEKLPNYMKGLYQAI 360

Query: 361 LEFFGEIEQDMSMDNNNIPFALDCAKEAV 388
           L+FFGEIE+DMSMDN+  PFALD AKE++
Sbjct: 361 LDFFGEIEEDMSMDNSTAPFALDSAKESL 389

BLAST of Cucsat.G852 vs. NCBI nr
Match: XP_011650452.2 ((-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germacrene D synthase isoform X1 [Cucumis sativus] >KAE8650088.1 hypothetical protein Csa_009890 [Cucumis sativus])

HSP 1 Score: 577 bits (1486), Expect = 6.85e-203
Identity = 280/282 (99.29%), Postives = 281/282 (99.65%), Query Frame = 0

Query: 107 MLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLL 166
           MLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIR DENGLL
Sbjct: 1   MLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENGLL 60

Query: 167 SLYEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMAR 226
           SLYEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMAR
Sbjct: 61  SLYEASHLRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMAR 120

Query: 227 HYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVE 286
           HYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVE
Sbjct: 121 HYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVE 180

Query: 287 CYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIE 346
           CYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIE
Sbjct: 181 CYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIE 240

Query: 347 KLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEAV 388
           KLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEA+
Sbjct: 241 KLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEAL 282

BLAST of Cucsat.G852 vs. NCBI nr
Match: XP_038877743.1 (LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida])

HSP 1 Score: 505 bits (1301), Expect = 2.63e-173
Identity = 261/385 (67.79%), Postives = 309/385 (80.26%), Query Frame = 0

Query: 5   MSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQE 64
           MSF+ V S+SMT N++S    +GVP RS  FQ S+ G +FLS++  TLEE++M+ K++QE
Sbjct: 1   MSFQIVVSDSMTPNNDSL--SHGVPRRSANFQPSAWGDHFLSYRYNTLEEEEMVMKQVQE 60

Query: 65  LKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYI 124
            KEEVR MF   +K+S+KLSLIDSIQ LGLSYHF DE +EV+  ++NP NVD+E EDLYI
Sbjct: 61  YKEEVRRMFGVAEKYSEKLSLIDSIQRLGLSYHFKDESSEVIDYIQNPTNVDEEDEDLYI 120

Query: 125 TALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDE 184
           TALRFRLLRQQGFFVSC+IFNKFTNE G+ KESI  D  G +SLYEASHLRMKGENILDE
Sbjct: 121 TALRFRLLRQQGFFVSCDIFNKFTNEMGDFKESIVKDGAGXISLYEASHLRMKGENILDE 180

Query: 185 ALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN-ALL 244
           ALAFTTT LKAMAMDS+SPF EEAKYALKWPIYKA+ RFM RHYISLY NN LKNN  LL
Sbjct: 181 ALAFTTTQLKAMAMDSSSPFSEEAKYALKWPIYKAMPRFMNRHYISLYQNNALKNNNVLL 240

Query: 245 TFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCAN 304
            FAKLD+N+LQKLYQKEL E SRWWK+ K   QL FARDR VECY+W +  + +P Y   
Sbjct: 241 KFAKLDFNTLQKLYQKELGEFSRWWKDLKLRMQLPFARDRGVECYVWALGIFYEPKYSNA 300

Query: 305 RRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFF 364
           R I  K++ F++++DDMYD YATL+ELQLFT+ I+RWD+  I+ LPNYMKGLYEAILE +
Sbjct: 301 RTILAKVIAFISLLDDMYDVYATLEELQLFTNLIERWDVNCIKMLPNYMKGLYEAILEVY 360

Query: 365 GEIEQDMSMDNNNIPFALDCAKEAV 388
           GEIEQD+  DNN IPFA D AKE +
Sbjct: 361 GEIEQDICKDNN-IPFAFDYAKETM 382

BLAST of Cucsat.G852 vs. NCBI nr
Match: XP_016900585.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 489 bits (1258), Expect = 9.16e-167
Identity = 262/393 (66.67%), Postives = 305/393 (77.61%), Query Frame = 0

Query: 5   MSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLS-HQPKTLEEDKMIRKK-L 64
           MS+E++     T+ +N SF+D  +  R+ +F  S  G YFLS H    L ED ++ K+  
Sbjct: 1   MSYEKI-----TAANNGSFSDGVLRRRAAEFPPSPWGDYFLSSHFNNQLVEDNVVEKQQF 60

Query: 65  QELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLM--LMK-NPCNVDDEH 124
           QE+KEEVRSMF   +  S++LSLIDSIQ LGLS+HF +EINEV++  + K    N +D  
Sbjct: 61  QEVKEEVRSMFAAVENSSERLSLIDSIQRLGLSHHFQEEINEVVLEHIEKFRKDNDNDAD 120

Query: 125 EDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGE 184
           E+LYI AL+FR+LRQQG++VSCEIFNKFTNERGE KESI NDE GLLSLYEASHLR KGE
Sbjct: 121 ENLYIIALQFRMLRQQGYYVSCEIFNKFTNERGEFKESIANDEVGLLSLYEASHLRTKGE 180

Query: 185 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLK- 244
           NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAV RFMAR YISL   NPLK 
Sbjct: 181 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVPRFMARQYISLCHKNPLKT 240

Query: 245 NNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKP 304
           NN LLTFAKLDYNSLQKLYQKEL E SRWWK  K  EQL FAR RVVE Y+W +    +P
Sbjct: 241 NNVLLTFAKLDYNSLQKLYQKELGEFSRWWKEHKLKEQLPFARVRVVEIYVWALGVQYEP 300

Query: 305 NYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEA 364
            YC  RRI  K++ F++++DDMYD YATLDELQLFT+AIQRWD+  IEKLPNYMKGLYE 
Sbjct: 301 KYCNTRRILAKIITFISVLDDMYDVYATLDELQLFTNAIQRWDVSCIEKLPNYMKGLYET 360

Query: 365 ILEFFGEIEQDMSMDNNNIPFALDCAKEAVNSI 391
           ILE + EIE+D+  DNN IPFA D AKEA+ S+
Sbjct: 361 ILEVYEEIEKDICKDNN-IPFAFDYAKEALKSL 387

BLAST of Cucsat.G852 vs. ExPASy TrEMBL
Match: A0A0A0L782 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1)

HSP 1 Score: 764 bits (1973), Expect = 1.24e-275
Identity = 383/388 (98.71%), Postives = 384/388 (98.97%), Query Frame = 0

Query: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60
           MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK
Sbjct: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60

Query: 61  KLQELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHE 120
           KLQELKEEVRSMFVTT KFSKKLSLIDSIQ LGLSYHFDDEINEVLMLMKNPCNVDDEHE
Sbjct: 61  KLQELKEEVRSMFVTTNKFSKKLSLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVDDEHE 120

Query: 121 DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGEN 180
           DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIR DEN LLSLYEASHLRMKGEN
Sbjct: 121 DLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRKDENSLLSLYEASHLRMKGEN 180

Query: 181 ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN 240
           ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN
Sbjct: 181 ILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNN 240

Query: 241 ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY 300
           ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY
Sbjct: 241 ALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNY 300

Query: 301 CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL 360
           CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL
Sbjct: 301 CANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAIL 360

Query: 361 EFFGEIEQDMSMDNNNIPFALDCAKEAV 388
           EFFGEIEQDMSMDNNNIPFALDCAKEA+
Sbjct: 361 EFFGEIEQDMSMDNNNIPFALDCAKEAL 388

BLAST of Cucsat.G852 vs. ExPASy TrEMBL
Match: A0A1S3BLW6 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1)

HSP 1 Score: 671 bits (1730), Expect = 3.62e-240
Identity = 333/389 (85.60%), Postives = 364/389 (93.57%), Query Frame = 0

Query: 1   MERKMSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRK 60
           MERKMSFEEV+S+SMTSNSNSSFNDNGVP  SEK+Q SSLGHYFLSHQPKTL+EDKMI+K
Sbjct: 1   MERKMSFEEVKSDSMTSNSNSSFNDNGVPRGSEKYQPSSLGHYFLSHQPKTLDEDKMIKK 60

Query: 61  KLQELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLML-MKNPCNVDDEH 120
           +LQELKEEVRSMFVT +KFS+KLSLIDSIQ LGLSYHFDDEINEVLML M+NPCN+D+EH
Sbjct: 61  QLQELKEEVRSMFVTIEKFSEKLSLIDSIQRLGLSYHFDDEINEVLMLLMQNPCNIDEEH 120

Query: 121 EDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGE 180
           EDLYITALRFRLLRQQG F+SCEIFNKFTNERGE KESI  D+ GLLSLYEASHLRMKGE
Sbjct: 121 EDLYITALRFRLLRQQGLFISCEIFNKFTNERGEFKESIGKDKIGLLSLYEASHLRMKGE 180

Query: 181 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKN 240
           NILDEALAFTT++LKAMAMDSNSPF+EEAKYALKWPIYKAV+R MARHYISLYSNNPLKN
Sbjct: 181 NILDEALAFTTSYLKAMAMDSNSPFYEEAKYALKWPIYKAVKRLMARHYISLYSNNPLKN 240

Query: 241 NALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPN 300
           N LLTFAKLDYNSLQKLYQ ELSEL+RWWKNQKFMEQLHFARDRVVECYLW +EA+ +P 
Sbjct: 241 NVLLTFAKLDYNSLQKLYQNELSELTRWWKNQKFMEQLHFARDRVVECYLWALEAFYEPK 300

Query: 301 YCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAI 360
           Y ANRRI LKM+ F+TIIDDMYD YAT+DELQLFT+AIQRWD KSIEKLPNYMKGLY+AI
Sbjct: 301 YSANRRILLKMIYFVTIIDDMYDAYATVDELQLFTEAIQRWDTKSIEKLPNYMKGLYQAI 360

Query: 361 LEFFGEIEQDMSMDNNNIPFALDCAKEAV 388
           L+FFGEIE+DMSMDN+  PFALD AKE++
Sbjct: 361 LDFFGEIEEDMSMDNSTAPFALDSAKESL 389

BLAST of Cucsat.G852 vs. ExPASy TrEMBL
Match: A0A1S4DXZ8 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1)

HSP 1 Score: 489 bits (1258), Expect = 4.44e-167
Identity = 262/393 (66.67%), Postives = 305/393 (77.61%), Query Frame = 0

Query: 5   MSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLS-HQPKTLEEDKMIRKK-L 64
           MS+E++     T+ +N SF+D  +  R+ +F  S  G YFLS H    L ED ++ K+  
Sbjct: 1   MSYEKI-----TAANNGSFSDGVLRRRAAEFPPSPWGDYFLSSHFNNQLVEDNVVEKQQF 60

Query: 65  QELKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLM--LMK-NPCNVDDEH 124
           QE+KEEVRSMF   +  S++LSLIDSIQ LGLS+HF +EINEV++  + K    N +D  
Sbjct: 61  QEVKEEVRSMFAAVENSSERLSLIDSIQRLGLSHHFQEEINEVVLEHIEKFRKDNDNDAD 120

Query: 125 EDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGE 184
           E+LYI AL+FR+LRQQG++VSCEIFNKFTNERGE KESI NDE GLLSLYEASHLR KGE
Sbjct: 121 ENLYIIALQFRMLRQQGYYVSCEIFNKFTNERGEFKESIANDEVGLLSLYEASHLRTKGE 180

Query: 185 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLK- 244
           NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAV RFMAR YISL   NPLK 
Sbjct: 181 NILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVPRFMARQYISLCHKNPLKT 240

Query: 245 NNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKP 304
           NN LLTFAKLDYNSLQKLYQKEL E SRWWK  K  EQL FAR RVVE Y+W +    +P
Sbjct: 241 NNVLLTFAKLDYNSLQKLYQKELGEFSRWWKEHKLKEQLPFARVRVVEIYVWALGVQYEP 300

Query: 305 NYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEA 364
            YC  RRI  K++ F++++DDMYD YATLDELQLFT+AIQRWD+  IEKLPNYMKGLYE 
Sbjct: 301 KYCNTRRILAKIITFISVLDDMYDVYATLDELQLFTNAIQRWDVSCIEKLPNYMKGLYET 360

Query: 365 ILEFFGEIEQDMSMDNNNIPFALDCAKEAVNSI 391
           ILE + EIE+D+  DNN IPFA D AKEA+ S+
Sbjct: 361 ILEVYEEIEKDICKDNN-IPFAFDYAKEALKSL 387

BLAST of Cucsat.G852 vs. ExPASy TrEMBL
Match: A0A6J1CQW1 ((-)-germacrene D synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 475 bits (1222), Expect = 1.27e-162
Identity = 247/384 (64.32%), Postives = 301/384 (78.39%), Query Frame = 0

Query: 5   MSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQE 64
           MSFE V  +++T+  N   ND GV  RS  FQ S  G YFL     T + D M+ KKLQ+
Sbjct: 1   MSFEVV--SNLTAPDNDPSND-GVLRRSANFQPSVWGDYFLFSHINT-DMDDMVGKKLQK 60

Query: 65  LKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYI 124
           LKEEVRSM    +K SKKLSLIDSIQ LGLS+HF++EINE+L  M+N  +++D+ ED+ I
Sbjct: 61  LKEEVRSMLAAAEKCSKKLSLIDSIQRLGLSHHFEEEINEILEQMQNXRSLNDDDEDVDI 120

Query: 125 TALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDE 184
            ALRFRLLRQ+G+ VSCEIFN+F N  G+ KES+  DE G+LSLYEASHLR+ GE+ILD+
Sbjct: 121 VALRFRLLRQRGYSVSCEIFNRFINGNGDFKESMVKDECGILSLYEASHLRINGEDILDK 180

Query: 185 ALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLT 244
           ALAFTTTHL+AMAMDS+SPF EEAKYALKWPIYKA+ RFM RHYISLY N+PLK+N LLT
Sbjct: 181 ALAFTTTHLQAMAMDSSSPFAEEAKYALKWPIYKALPRFMTRHYISLYDNDPLKSNVLLT 240

Query: 245 FAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANR 304
           F+KLD+N+LQKLYQKEL E SRWWK+ K MEQ  FARDRVVECY W +  Y +P Y   R
Sbjct: 241 FSKLDFNALQKLYQKELVEFSRWWKDLKLMEQFSFARDRVVECYFWTLGIYYEPKYSNAR 300

Query: 305 RIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFG 364
           RI +K++ F++++DDM D YATLDELQLFTDAI+RWDI  I+KLPNYMK + EAIL+ + 
Sbjct: 301 RILVKVIAFISLLDDMXDAYATLDELQLFTDAIERWDINGIDKLPNYMKVIXEAILQVYR 360

Query: 365 EIEQDMSMDNNNIPFALDCAKEAV 388
           E+EQD+  DNN I F LDCAKE++
Sbjct: 361 EVEQDIGKDNN-ILFGLDCAKESM 379

BLAST of Cucsat.G852 vs. ExPASy TrEMBL
Match: A0A5A7VDK3 (Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G005040 PE=4 SV=1)

HSP 1 Score: 476 bits (1226), Expect = 3.00e-162
Identity = 251/387 (64.86%), Postives = 295/387 (76.23%), Query Frame = 0

Query: 5   MSFEEVESNSMTSNSNSSFNDNGVPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQE 64
           MSF+ + S S   N   + +DNGV  R+E  Q S+ G YFLS+    LEED ++ KKL+E
Sbjct: 1   MSFQTIASESEAPNK-LTLSDNGVLRRTEYHQPSAWGDYFLSNLSNILEEDSVL-KKLEE 60

Query: 65  LKEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNPCNVDDE-HEDLY 124
           LKE  RSMFV  +K S KL LIDSIQCLGLSYHF+DEINE+L  ++N  +VD+E  EDLY
Sbjct: 61  LKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYHFEDEINEILDQIQNSAHVDEEDEEDLY 120

Query: 125 ITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILD 184
           I AL+FRLLRQQGF VSCEIFNKFT E G+ KESI  DE GLLSLYEASHLRMKGE+I+D
Sbjct: 121 IIALKFRLLRQQGFPVSCEIFNKFTTESGDFKESITKDEKGLLSLYEASHLRMKGESIMD 180

Query: 185 EALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKN--NA 244
           EAL FTTT L+AMAMDS SPF +EAKYALKWPIYK + RFM RHYISLY NNP KN  N 
Sbjct: 181 EALEFTTTQLEAMAMDSTSPFSQEAKYALKWPIYKTLSRFMTRHYISLYHNNPTKNDNNI 240

Query: 245 LLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYC 304
           LLTFAKLDYNSLQKLYQ+EL ELSRWW + K  E+L FARDRVVE Y+W +  Y +P Y 
Sbjct: 241 LLTFAKLDYNSLQKLYQRELGELSRWWNDSKLKEKLPFARDRVVESYIWALGIYYEPKYS 300

Query: 305 ANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILE 364
           + R +  K++  +T+IDDMYD Y TL+ELQLFT+ I RWD+  IE+LPNYMK LYEAIL+
Sbjct: 301 SARTLLAKIIALITLIDDMYDCYGTLEELQLFTNTIGRWDMNCIEELPNYMKVLYEAILK 360

Query: 365 FFGEIEQDMSMDNNNIPFALDCAKEAV 388
            +    QDM MDNN IP+A D AKE +
Sbjct: 361 IYEGTIQDMCMDNN-IPYAFDYAKEGI 384

BLAST of Cucsat.G852 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 223.0 bits (567), Expect = 4.2e-58
Identity = 121/325 (37.23%), Postives = 189/325 (58.15%), Query Frame = 0

Query: 66  KEEVRSMFVTTKKFSKKLSLIDSIQCLGLSYHFDDEINEVLMLMKNP-CNVDDEHEDLYI 125
           KEEVR      +   ++L +IDS+Q LG+SYH+  EI+++L  + +    ++ E +DL+ 
Sbjct: 28  KEEVRKTLNEIEGSIEQLEMIDSLQRLGISYHYKHEIHDILRKIHDQHGEIERETQDLHA 87

Query: 126 TALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDE 185
           T+L F LLRQ GF VS + F+ F +E GE ++++ +D  GLLSLYEAS+  M  E  L E
Sbjct: 88  TSLEFILLRQHGFDVSQDAFDVFISETGEFRKTLHSDIKGLLSLYEASYFSMDSEFKLKE 147

Query: 186 ALAFTTTHL--------KAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNP 245
              +    L        K +  +  +   E  K AL+ P + +++R  AR YI++Y    
Sbjct: 148 TRIYANKRLSEFVAESSKTICREDETYILEMVKRALETPYHWSIRRLEARWYINVYEKKH 207

Query: 246 LKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYN 305
             N  LL FA +D+N LQ  +Q+EL  +S WW +   M+QL F RDR+ E Y W I  + 
Sbjct: 208 EMNPLLLEFAAIDFNMLQANHQEELKLISSWWNSTGLMKQLDFVRDRITESYFWTIGIFY 267

Query: 306 KPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLY 365
           +P +   R+I  K+   + I+DD+YD Y TL+EL+LFT+ +++WD+  +E+LPNYM+  +
Sbjct: 268 EPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELFTNVVEKWDVNHVERLPNYMRMCF 327

Query: 366 EAILEFFGEIEQDMSMDN--NNIPF 380
             +     +I  D+  D   N IP+
Sbjct: 328 LFLYNEINQIGYDVLRDKGLNVIPY 352

BLAST of Cucsat.G852 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 213.4 bits (542), Expect = 3.3e-55
Identity = 117/323 (36.22%), Postives = 189/323 (58.51%), Query Frame = 0

Query: 59  RKKLQELKEEVRSMFVTTKKFS-KKLSLIDSIQCLGLSYHFDDEINEVL----MLMKNPC 118
           ++K   LKE V+  F+++K    + +  ID++  LG+SYHF+ +I E L      +  P 
Sbjct: 35  KEKHSTLKEAVKEAFMSSKANPIENIKFIDALCRLGVSYHFEKDIVEQLDKSFDCLDFPQ 94

Query: 119 NVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASH 178
            V  E  DLY   + F++ RQ GF +S ++F KF +E G+ K  +  D  G+LSLYEA+ 
Sbjct: 95  MVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQ 154

Query: 179 LRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYS 238
               GE+I+DEALAF+ +HL+ ++  S+       K ALK P +K + R   R YIS Y 
Sbjct: 155 WGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISYYE 214

Query: 239 NNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIE 298
                +  LL FAK+D+N LQ L+++EL+ ++RW    +F  ++ + R R+ E YLW + 
Sbjct: 215 EEESCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLG 274

Query: 299 AYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMK 358
            Y +P Y   R I    +   T +DDMYD Y T++EL+LFTDA+  + I +I  +P+ MK
Sbjct: 275 TYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEYSICNIIPIPDSMK 334

Query: 359 GLYEAILEFFGEIEQDMSMDNNN 377
            +Y   +EF+ ++++++  +  +
Sbjct: 335 FIYNVTVEFYDKLDEELEKEGRS 357

BLAST of Cucsat.G852 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 208.0 bits (528), Expect = 1.4e-53
Identity = 116/325 (35.69%), Postives = 187/325 (57.54%), Query Frame = 0

Query: 59  RKKLQELKEEVRSMFVTTKKFS-KKLSLIDSIQCLGLSYHFDDEINEVL----MLMKNPC 118
           ++K   LKE V+  F+++K    + +  ID++  LG+SYHF+ +I E L      +  P 
Sbjct: 35  KEKHSTLKEAVKEAFMSSKANPIENIKFIDALCRLGVSYHFEKDIVEQLDKSFDCLDFPQ 94

Query: 119 NVDDEHEDLYITALRFRLLRQQGFFVSCEIFNKFTNERGELKESIRNDENGLLSLYEASH 178
            V  E  DLY   + F++ RQ GF +S ++F KF +E G+ K  +  D  G+LSLYEA+ 
Sbjct: 95  MVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLSLYEAAQ 154

Query: 179 LRMKGENILDEALAFTTTHLKAMAMDSNSPFFEEAKYALKWPIYKAVQRFMARHYISLYS 238
               GE+I+DEALAF+ +HL+ ++  S+       K ALK P +K + R   R YIS Y 
Sbjct: 155 WGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQYISYYE 214

Query: 239 NNPLKNNALLTFAKLDYNSLQKLYQKELSELSRWWKNQKFMEQLHFARDRVVECYLWVIE 298
                +  LL FAK+D+N LQ L+++EL+ ++RW    +F  ++ + R R+ E YLW + 
Sbjct: 215 EEESCDPTLLEFAKIDFNLLQILHREELACVTRWHHEMEFKSKVTYTRHRITEAYLWSLG 274

Query: 299 AYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLFTDAIQRW--DIKSIEKLPNY 358
            Y +P Y   R I    +   T +DDMYD Y T++EL+LFTDA+  W   +     +P+ 
Sbjct: 275 TYFEPQYSQARVITTMALILFTALDDMYDAYGTMEELELFTDAMDEWLPVVPDEIPIPDS 334

Query: 359 MKGLYEAILEFFGEIEQDMSMDNNN 377
           MK +Y   +EF+ ++++++  +  +
Sbjct: 335 MKFIYNVTVEFYDKLDEELEKEGRS 359

BLAST of Cucsat.G852 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 206.5 bits (524), Expect = 4.1e-53
Identity = 136/363 (37.47%), Postives = 194/363 (53.44%), Query Frame = 0

Query: 28  VPHRSEKFQSSSLGHYFLSHQPKTLEEDKMIRKKLQELKEEVRSMFVTTKK-FSKKLSLI 87
           V  RS  +Q S   H+ L        +DK +R++   LKE+VR M    +K +  +L  I
Sbjct: 44  VQRRSANYQPSRWDHHHLLSVENKFAKDKRVRER-DLLKEKVRKMLNDEQKTYLDQLEFI 103

Query: 88  DSIQCLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIFNK 147
           D +Q LG+SYHF+ EI+ +L         + +  DL+ TAL FRL RQ GF VS ++F+ 
Sbjct: 104 DDLQKLGVSYHFEAEIDNILTSSYKKDRTNIQESDLHATALEFRLFRQHGFNVSEDVFDV 163

Query: 148 FTNERGELKESIRNDENGLLSLYEASHLRMK-GENILDEALAFTTTHLKAMA-MDSNSPF 207
           F    G+     R+D  GL+SLYEAS+L  K  +N+      F T  L+      SN  F
Sbjct: 164 FMENCGKFD---RDDIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRDFVDTHSNEDF 223

Query: 208 -----FEEAKYALKWPIYKAVQRFMARHYISLYSNNP-LKNNALLTFAKLDYNSLQKLYQ 267
                 E    AL  P Y  ++R   R YI +Y      KN  ++ FAK+D+N +Q ++Q
Sbjct: 224 GSCDMVEIVVQALDMPYYWQMRRLSTRWYIDVYGKRQNYKNLVVVEFAKIDFNIVQAIHQ 283

Query: 268 KELSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIID 327
           +EL  +S WW      +QL+FARDR+VE Y W I    +P Y   R+   K+   LT ID
Sbjct: 284 EELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTKINALLTTID 343

Query: 328 DMYDTYATLDELQLFTDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDM--SMDNNN 380
           D+YD Y TL+ELQLFT A + WDI  +++LP YM+  +  I      I  ++  + + N 
Sbjct: 344 DIYDIYGTLEELQLFTVAFENWDINRLDELPEYMRLCFLVIYNEVNSIACEILRTKNINV 402

BLAST of Cucsat.G852 vs. TAIR 10
Match: AT3G25820.1 (terpene synthase-like sequence-1,8-cineole )

HSP 1 Score: 204.9 bits (520), Expect = 1.2e-52
Identity = 129/333 (38.74%), Postives = 190/333 (57.06%), Query Frame = 0

Query: 31  RSEKFQSSSLGH-YFLSHQPKTLEEDKMIRKKLQELKEEVRSMFVTTKKFS-KKLSLIDS 90
           RS  +Q S   H Y LS + K   E ++I + +  LK++V+SM    KK   ++L LID 
Sbjct: 48  RSGNYQPSPWNHCYLLSIENKYASETEVITRDV--LKKKVKSMLDDEKKSRLEQLELIDD 107

Query: 91  IQCLGLSYHFDDEINEVL----MLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEIF 150
           +Q LG+SYHF+ EIN+ L    + M   C   D+ EDL+ T+L FRLLRQ GF VS  IF
Sbjct: 108 LQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKEEDLHATSLEFRLLRQHGFDVSENIF 167

Query: 151 NKFTNERGELKESIRNDENGLLSLYEASHLRMKGENILDEAL-AFTTTHLKAMAMD---S 210
           +   ++  E      N+ NG++SLYEAS+L  K +  L + +  F T  ++    D    
Sbjct: 168 DVIIDQI-ESNTFKTNNINGIISLYEASYLSTKSDTKLHKVIRPFATEQIRKFVDDEDTK 227

Query: 211 NSPFFEEAKYALKWPIYKAVQRFMARHYISLYSNNPLKNNALLTFAKLDYNSLQKLYQKE 270
           N    E+A +AL+ P +  ++R   R YI  Y      N  L+ FAK+D+N +Q  +Q++
Sbjct: 228 NIEVREKAYHALEMPYHWRMRRLDTRWYIDAYEKKHDMNLVLIEFAKIDFNIVQAAHQED 287

Query: 271 LSELSRWWKNQKFMEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDM 330
           L  +SRWWK+     QL F RDR+VE Y W +    +P +   RRI   +   +T IDD+
Sbjct: 288 LKYVSRWWKDTCLTNQLPFVRDRIVENYFWTVGLIYEPQFGYIRRIMTIVNALVTTIDDI 347

Query: 331 YDTYATLDELQLFTDAIQRWDIKSIEKLPNYMK 354
           YD Y TL+EL+LFT  ++ WD+  + +LP YM+
Sbjct: 348 YDIYGTLEELELFTSMVENWDVNRLGELPEYMR 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6Q3H31.8e-9349.18(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S... [more]
B9SCB69.7e-8443.55Probable terpene synthase 2 OS=Ricinus communis OX=3988 GN=TPS2 PE=3 SV=1[more]
O498534.1e-8246.78(+)-delta-cadinene synthase isozyme C2 OS=Gossypium arboreum OX=29729 GN=CAD1-C2... [more]
P936656.9e-8246.50(+)-delta-cadinene synthase OS=Gossypium hirsutum OX=3635 GN=CDN1 PE=1 SV=1[more]
B2KSJ51.5e-8147.06(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_031738277.13.12e-27698.97(-)-germacrene D synthase isoform X2 [Cucumis sativus][more]
XP_008448975.17.47e-24085.60PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
XP_011650452.26.85e-20399.29(-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germa... [more]
XP_038877743.12.63e-17367.79LOW QUALITY PROTEIN: (-)-germacrene D synthase-like [Benincasa hispida][more]
XP_016900585.19.16e-16766.67PREDICTED: (-)-germacrene D synthase-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0L7821.24e-27598.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1[more]
A0A1S3BLW63.62e-24085.60(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1[more]
A0A1S4DXZ84.44e-16766.67(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103491132 PE=4 SV=1[more]
A0A6J1CQW11.27e-16264.32(-)-germacrene D synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5A7VDK33.00e-16264.86Putative terpene synthase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT4G16730.14.2e-5837.23terpene synthase 02 [more]
AT5G23960.23.3e-5536.22terpene synthase 21 [more]
AT5G23960.11.4e-5335.69terpene synthase 21 [more]
AT2G24210.14.1e-5337.47terpene synthase 10 [more]
AT3G25820.11.2e-5238.74terpene synthase-like sequence-1,8-cineole [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 1..62
e-value: 2.3E-79
score: 269.0
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 63..215
e-value: 2.3E-79
score: 269.0
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 64..198
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 68..213
e-value: 7.7E-53
score: 179.5
NoneNo IPR availablePANTHERPTHR31225:SF425-EPIARISTOLOCHENE SYNTHASEcoord: 4..216
NoneNo IPR availablePANTHERPTHR31225OS04G0344100 PROTEIN-RELATEDcoord: 4..216
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 2..60

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G852.T1Cucsat.G852.T1mRNA
Cucsat.G852.T24Cucsat.G852.T24mRNA
Cucsat.G852.T6Cucsat.G852.T6mRNA
Cucsat.G852.T5Cucsat.G852.T5mRNA
Cucsat.G852.T4Cucsat.G852.T4mRNA
Cucsat.G852.T3Cucsat.G852.T3mRNA
Cucsat.G852.T2Cucsat.G852.T2mRNA
Cucsat.G852.T8Cucsat.G852.T8mRNA
Cucsat.G852.T19Cucsat.G852.T19mRNA
Cucsat.G852.T12Cucsat.G852.T12mRNA
Cucsat.G852.T11Cucsat.G852.T11mRNA
Cucsat.G852.T17Cucsat.G852.T17mRNA
Cucsat.G852.T14Cucsat.G852.T14mRNA
Cucsat.G852.T20Cucsat.G852.T20mRNA
Cucsat.G852.T7Cucsat.G852.T7mRNA
Cucsat.G852.T9Cucsat.G852.T9mRNA
Cucsat.G852.T13Cucsat.G852.T13mRNA
Cucsat.G852.T16Cucsat.G852.T16mRNA
Cucsat.G852.T26Cucsat.G852.T26mRNA
Cucsat.G852.T25Cucsat.G852.T25mRNA
Cucsat.G852.T23Cucsat.G852.T23mRNA
Cucsat.G852.T28Cucsat.G852.T28mRNA
Cucsat.G852.T18Cucsat.G852.T18mRNA
Cucsat.G852.T15Cucsat.G852.T15mRNA
Cucsat.G852.T21Cucsat.G852.T21mRNA
Cucsat.G852.T10Cucsat.G852.T10mRNA
Cucsat.G852.T27Cucsat.G852.T27mRNA
Cucsat.G852.T22Cucsat.G852.T22mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016102 diterpenoid biosynthetic process
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0010333 terpene synthase activity
molecular_function GO:0016829 lyase activity