Cucsat.G8476 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G8476
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	NB-ARC domain-containing protein
Location	ctg1557: 2898889 .. 2899652 (-)
RNA-Seq Expression	Cucsat.G8476
Synteny	Cucsat.G8476

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAATGGTTGAATACAAAGTGGAGTTTCTTGATAATAAAAGTGCCATGACACTCTTTTGCAAACAAGCATTTGGATCATGTGATCAGTTTCCCAGTAAGAATTTTGAGGACTTTTCTAAGGAGATTGTAGAAAGGGTTAAAGGACATCCACAGGTTTTGAGACAAATTGGGTCGTCTTTATATGATAAAGGTATAGAGATATGGAAAGAACAATTGAAGAGTCTTGAGGAAGATTACAACAATCGTATATTTAAGACATTAAAGATAAGTTTTGATGATTTAGGAAAGACAAGCCAAGAAGTTTTTCTTGATTTTGCATGCTTCTTCAATGAGAAGAAGAAAGAGAGTGTGATTGAAATACTTAAGAGTCTTGATTATAGACCTCATAGCGAAATACAATTGTTGGAAGATAGATGTCTCATTGAAGTAAGACGTGACAACACAATATTTATGCCTAAGTGCATTCAAGCTATGGGTCAACAGATTGAACGTGAAGCTGATAAACGGAGTAGGATTTGGCTTCCGAAAGATGCCCATGATGTATTTGATGAACCACATGTAAGACTGAAATATAAAAGTTTATATATATACATATATATATATAAATGTTTGTTTATCTTTCCCTTTCTTATATGTTTTAAATAATGTTTATTTACAGAGAGTAAAGGACATAAAAGGTGTAGTCTTGAAATTGGAAGAGAAGCAAGAAGAAGTAAAGTTAGAGGGTAAGGTTTTTGAGGATATGAGAAGTTTAAAAATATTG

Coding sequence (CDS)

AAAATGGTTGAATACAAAGTGGAGTTTCTTGATAATAAAAGTGCCATGACACTCTTTTGCAAACAAGCATTTGGATCATGTGATCAGTTTCCCAGTAAGAATTTTGAGGACTTTTCTAAGGAGATTGTAGAAAGGGTTAAAGGACATCCACAGGTTTTGAGACAAATTGGGTCGTCTTTATATGATAAAGGTATAGAGATATGGAAAGAACAATTGAAGAGTCTTGAGGAAGATTACAACAATCGTATATTTAAGACATTAAAGATAAGTTTTGATGATTTAGGAAAGACAAGCCAAGAAGTTTTTCTTGATTTTGCATGCTTCTTCAATGAGAAGAAGAAAGAGAGTGTGATTGAAATACTTAAGAGTCTTGATTATAGACCTCATAGCGAAATACAATTGTTGGAAGATAGATGTCTCATTGAAGTAAGACGTGACAACACAATATTTATGCCTAAGTGCATTCAAGCTATGGGTCAACAGATTGAACGTGAAGCTGATAAACGGAGTAGGATTTGGCTTCCGAAAGATGCCCATGATGTATTTGATGAACCACATAGAGTAAAGGACATAAAAGGTGTAGTCTTGAAATTGGAAGAGAAGCAAGAAGAAGTAAAGTTAGAGGGTAAGGTTTTTGAGGATATGAGAAGTTTAAAAATATTG

Protein sequence

KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHDVFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL

Homology

[Close]

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.2e-22
Identity = 60/180 (33.33%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 5   YKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKG 64
           Y+V  L +  ++ LF + AFG   + P++NFE  S E+V   KG P  L+  GS L++  
Sbjct: 343 YEVTALPDHESIQLFKQHAFGK--EVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLR 402

Query: 65  IEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKSL 124
           +  WK  ++ ++ +  + I   LKIS+D L    QE+FLD ACF   ++K+ +++IL+S 
Sbjct: 403 LTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESC 462

Query: 125 DYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQI---EREADKRSRIWLPKDAHDV 182
                  +++L D+ L+ +   N + M   IQ MG+ I   +++  +RSR+WL K+  +V
Sbjct: 463 HIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEV 520

[Close]

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot
Match: Q40253 (Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.5e-21
Identity = 66/222 (29.73%), Postives = 111/222 (50.00%), Query Frame = 0


Query: 5   YKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKG 64
           Y+V  +    ++ LF K AF      P   +E  + ++V+   G P  L+ IGS L+ + 
Sbjct: 394 YEVGSMSKPRSLELFSKHAFKK--NTPPSYYETLANDVVDTTAGLPLTLKVIGSLLFKQE 453

Query: 65  IEIWKEQLKSLEEDYN-NRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKS 124
           I +W++ L+ L    N + ++  LKIS+D L   ++E+FLD ACFF  + KE    +   
Sbjct: 454 IAVWEDTLEQLRRTLNLDEVYDRLKISYDALNPEAKEIFLDIACFFIGQNKEEPYYMWTD 513

Query: 125 LDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREAD----KRSRIWLPKDAH 184
            ++ P S I  L  RC+I+V  D+   M   ++ MG++I R  D    KRSRIW  ++  
Sbjct: 514 CNFYPASNIIFLIQRCMIQVGDDDEFKMHDQLRDMGREIVRREDVLPWKRSRIWSAEEGI 573

Query: 185 DVFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 222
           D+         +K + +    K E    + + F ++  L+ L
Sbjct: 574 DLLLNKKGSSKVKAISIPWGVKYE---FKSECFLNLSELRYL 610

[Close]

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 73/222 (32.88%), Postives = 109/222 (49.10%), Query Frame = 0


Query: 5   YKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKG 64
           Y+VE L+ + A  LF   AF      P  ++ + +  +V   +G P  L+ +GS L  K 
Sbjct: 350 YEVEGLNFEEACELFSLYAFK--QNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKT 409

Query: 65  IEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKSL 124
           I  W+ +LK L+ +    I K LK S+D L +  + +FLD ACFF  + ++ V+ IL   
Sbjct: 410 IPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGC 469

Query: 125 DYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIER-----EADKRSRIWLPKDAH 184
           D+   + I  L D CLI +   N I M   IQ MG +I R     E +K SR+W P D  
Sbjct: 470 DFPAETGISNLNDLCLITLPY-NQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFE 529

Query: 185 DVFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 222
                   +K ++ + L L  K + V     VF  M  L++L
Sbjct: 530 RALTADEGIKSVETMSLDL-SKLKRVCSNSNVFAKMTKLRLL 567

[Close]

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.2e-20
Identity = 66/221 (29.86%), Postives = 111/221 (50.23%), Query Frame = 0


Query: 5   YKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKG 64
           Y+++ L+   A+ LF   AF      P K F++    +V+   G P  L+ +GS LY + 
Sbjct: 343 YEIKVLEKDEAIELFNLHAFKRSS--PEKEFKELLNLVVDYTGGLPLALKVLGSLLYKED 402

Query: 65  IEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKSL 124
           +++W   +  L+++    I  TLKISFD L    + +FLD ACFF    +  +  +  + 
Sbjct: 403 LDVWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHAS 462

Query: 125 DYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHDVFDE 184
            + P   ++ L ++ LI +  D  I M   +Q MG+QI  +     RI+ P+D  D    
Sbjct: 463 GFHPVLGVKTLVEKSLIFILEDK-IQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIG 522

Query: 185 PHRVKDIKGVVLKLEEKQEEVKLE----GKVFEDMRSLKIL 222
             R + I+G++L   E+ EE +LE     +  +  R L+IL
Sbjct: 523 DMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRIL 560

[Close]

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.2e-20
Identity = 70/222 (31.53%), Postives = 110/222 (49.55%), Query Frame = 0


Query: 5   YKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSLYDKG 64
           Y+V+ L+ + A  LF   AF      P  ++ + S  +V   +G P  L+ +G  L  K 
Sbjct: 355 YEVKGLNFEEACELFSLYAFE--QNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKT 414

Query: 65  IEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEILKSL 124
           I  W+ +L+ L+ +    I   LK S+D LG+T + +FLD ACFF  + ++ V +IL + 
Sbjct: 415 IPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDAC 474

Query: 125 DYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIER-----EADKRSRIWLPKDAH 184
           D+     I+ L D+CLI ++  N I M   IQ MG +I R     E +K SR+W   D  
Sbjct: 475 DFHAEIGIKNLNDKCLITLQY-NRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFE 534

Query: 185 DVFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 222
                   +K ++ + L L  K + V      F  M  L++L
Sbjct: 535 RALTAYKGIKRVETISLDL-SKLKRVCSNSNAFAKMTRLRLL 572

[Close]

BLAST of Cucsat.G8476 vs. NCBI nr
Match: XP_031741448.1 (protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 [Cucumis sativus])

HSP 1 Score: 442 bits (1138), Expect = 2.18e-151
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL
Sbjct: 140 KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 199

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI
Sbjct: 200 YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 259

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD
Sbjct: 260 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 319

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL
Sbjct: 320 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 360

[Close]

BLAST of Cucsat.G8476 vs. NCBI nr
Match: KAE8648984.1 (hypothetical protein Csa_008421 [Cucumis sativus])

HSP 1 Score: 442 bits (1138), Expect = 8.55e-149
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL
Sbjct: 340 KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 399

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI
Sbjct: 400 YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 459

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD
Sbjct: 460 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 519

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL
Sbjct: 520 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 560

[Close]

BLAST of Cucsat.G8476 vs. NCBI nr
Match: KAA0039322.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00505.1 TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 392 bits (1008), Expect = 5.53e-132
Identity = 199/221 (90.05%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY VEFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERV+GHPQ L QIGSSL
Sbjct: 58  KMVEYSVEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVEGHPQALIQIGSSL 117

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLD ACFFNEK KE VIEI
Sbjct: 118 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDLACFFNEKTKEKVIEI 177

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTI MPKCIQ MGQQIEREADKRSRIWLPKDA D
Sbjct: 178 LKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKCIQTMGQQIEREADKRSRIWLPKDAQD 237

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 238 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 276

[Close]

BLAST of Cucsat.G8476 vs. NCBI nr
Match: AGH33848.2 (resistance gene-like protein [Cucumis melo])

HSP 1 Score: 392 bits (1008), Expect = 6.25e-132
Identity = 199/221 (90.05%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY VEFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERV+GHPQ L QIGSSL
Sbjct: 144 KMVEYSVEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVEGHPQALIQIGSSL 203

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLD ACFFNEK KE VIEI
Sbjct: 204 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDLACFFNEKTKEKVIEI 263

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTI MPKCIQ MGQQIEREADKRSRIWLPKDA D
Sbjct: 264 LKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKCIQTMGQQIEREADKRSRIWLPKDAQD 323

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 324 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 362

[Close]

BLAST of Cucsat.G8476 vs. NCBI nr
Match: AAU04764.1 (MRGH9 [Cucumis melo])

HSP 1 Score: 391 bits (1005), Expect = 1.53e-129
Identity = 198/221 (89.59%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY +EFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERVKGHPQ L QIGSSL
Sbjct: 341 KMVEYSMEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVKGHPQALIQIGSSL 400

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDL KTSQEVFLD ACFFNEK KE VIEI
Sbjct: 401 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLEKTSQEVFLDLACFFNEKTKEKVIEI 460

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTIFMPKCIQ MGQQIEREADKRSRIW+PKDA D
Sbjct: 461 LKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKCIQTMGQQIEREADKRSRIWIPKDAQD 520

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 521 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 559

[Close]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL
Match: A0A0A0KXU3 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647580 PE=4 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 1.60e-154
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL
Sbjct: 58  KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 117

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI
Sbjct: 118 YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 177

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD
Sbjct: 178 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 237

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL
Sbjct: 238 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 278

[Close]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL
Match: A0A5D3BN34 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001150 PE=4 SV=1)

HSP 1 Score: 392 bits (1008), Expect = 2.68e-132
Identity = 199/221 (90.05%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY VEFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERV+GHPQ L QIGSSL
Sbjct: 58  KMVEYSVEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVEGHPQALIQIGSSL 117

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLD ACFFNEK KE VIEI
Sbjct: 118 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDLACFFNEKTKEKVIEI 177

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTI MPKCIQ MGQQIEREADKRSRIWLPKDA D
Sbjct: 178 LKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKCIQTMGQQIEREADKRSRIWLPKDAQD 237

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 238 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 276

[Close]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL
Match: Q5DMW2 (MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1)

HSP 1 Score: 391 bits (1005), Expect = 7.40e-130
Identity = 198/221 (89.59%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY +EFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERVKGHPQ L QIGSSL
Sbjct: 341 KMVEYSMEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVKGHPQALIQIGSSL 400

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDL KTSQEVFLD ACFFNEK KE VIEI
Sbjct: 401 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLEKTSQEVFLDLACFFNEKTKEKVIEI 460

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTIFMPKCIQ MGQQIEREADKRSRIW+PKDA D
Sbjct: 461 LKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKCIQTMGQQIEREADKRSRIWIPKDAQD 520

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 521 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 559

[Close]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL
Match: A0A1S3C9Z0 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1)

HSP 1 Score: 391 bits (1005), Expect = 2.35e-129
Identity = 198/221 (89.59%), Postives = 205/221 (92.76%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY +EFLD KSAMTLFCK AFG C  FPSKNFEDFSKEIVERVKGHPQ L QIGSSL
Sbjct: 341 KMVEYSMEFLDTKSAMTLFCKHAFG-CG-FPSKNFEDFSKEIVERVKGHPQALIQIGSSL 400

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDL KTSQEVFLD ACFFNEK KE VIEI
Sbjct: 401 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLEKTSQEVFLDLACFFNEKTKEKVIEI 460

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTIFMPKCIQ MGQQIEREADKRSRIW+PKDA D
Sbjct: 461 LKSFDYRPHSEIQLLQDRCLIEVRSDNTIFMPKCIQTMGQQIEREADKRSRIWIPKDAQD 520

Query: 181 VFDEPHRVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPHRVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 521 VFDEPHRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 559

[Close]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL
Match: M4QSV9 (Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1)

HSP 1 Score: 386 bits (991), Expect = 8.88e-128
Identity = 198/222 (89.19%), Postives = 204/222 (91.89%), Query Frame = 0


Query: 1   KMVEYKVEFLDNKSAMTLFCKQAFGSCDQFPSKNFEDFSKEIVERVKGHPQVLRQIGSSL 60
           KMVEY VEFLD KSAMTLFCK AFG C  FP KNF+DFSKEIVERVKGHPQ L QIGSSL
Sbjct: 337 KMVEYSVEFLDTKSAMTLFCKHAFG-CG-FPRKNFKDFSKEIVERVKGHPQALIQIGSSL 396

Query: 61  YDKGIEIWKEQLKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDFACFFNEKKKESVIEI 120
           YDKGIEIWKE+LKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLD ACFFNEK KE VIEI
Sbjct: 397 YDKGIEIWKEELKSLEEDYNNRIFKTLKISFDDLGKTSQEVFLDLACFFNEKTKEKVIEI 456

Query: 121 LKSLDYRPHSEIQLLEDRCLIEVRRDNTIFMPKCIQAMGQQIEREADKRSRIWLPKDAHD 180
           LKS DYRPHSEIQLL+DRCLIEVR DNTI MPKCIQ MGQQIEREADKRSRIWLPKDA D
Sbjct: 457 LKSFDYRPHSEIQLLQDRCLIEVRSDNTILMPKCIQTMGQQIEREADKRSRIWLPKDAQD 516

Query: 181 VFDEPH-RVKDIKGVVLKLEEKQEEVKLEGKVFEDMRSLKIL 221
           VFDEPH RVKDIKGVVLKLEEKQ+E++LEGKVFEDMRSLKIL
Sbjct: 517 VFDEPHQRVKDIKGVVLKLEEKQDEIELEGKVFEDMRSLKIL 556

The following BLAST results are available for this feature:

BLAST of Cucsat.G8476 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q40392	1.2e-22	33.33	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q40253	6.5e-21	29.73	Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1	[more]
V9M2S5	1.1e-20	32.88	Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=...	[more]
A0A290U7C4	4.2e-20	29.86	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
V9M398	4.2e-20	31.53	Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=...	[more]

BLAST of Cucsat.G8476 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741448.1	2.18e-151	100.00	protein SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 [Cucumis sativus]	[more]
KAE8648984.1	8.55e-149	100.00	hypothetical protein Csa_008421 [Cucumis sativus]	[more]
KAA0039322.1	5.53e-132	90.05	TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00505.1 TMV resista...	[more]
AGH33848.2	6.25e-132	90.05	resistance gene-like protein [Cucumis melo]	[more]
AAU04764.1	1.53e-129	89.59	MRGH9 [Cucumis melo]	[more]

BLAST of Cucsat.G8476 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KXU3	1.60e-154	100.00	NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647580 PE=4...	[more]
A0A5D3BN34	2.68e-132	90.05	TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
Q5DMW2	7.40e-130	89.59	MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1	[more]
A0A1S3C9Z0	2.35e-129	89.59	TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1	[more]
M4QSV9	8.88e-128	89.19	Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042197	Apoptotic protease-activating factors, helical domain	GENE3D	1.10.8.430		coord: 9..95 e-value: 3.4E-12 score: 47.8
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 5..81 e-value: 2.7E-6 score: 26.7
IPR044974	Disease resistance protein, plants	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 10..221
None	No IPR available	PANTHER	PTHR11017:SF243	RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATED	coord: 10..221
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 82..181
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 4..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G8476.T1	Cucsat.G8476.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0003953	NAD+ nucleosidase activity