Cucsat.G8436 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G8436
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Phloem lectin
Location	ctg1557: 2156409 .. 2158686 (-)
RNA-Seq Expression	Cucsat.G8436
Synteny	Cucsat.G8436

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTAATTACCATGTCCCATCTTTGAAACAATAAGATACAAAACAAGAGAGTATATATATGTGCCTTACACCACTCTCATCTTCATCCATCTCTCCAATCTCTTAATACTCCAAATTAACCTTTTCAAATATGGTTTGCATAGAGACAGAAGCAAGAGAGAGCTTGCAAATACAGGAGAGTTATGGTCATTGTCTTACTTATATTTTGCCAAAAACTCTAAATGTCACGGTGCATTGGCCTACCTATGCTAAGCTCTATCAACAACTTATTGCTGGAATCACCCTAAACAAGGGAACTAAGGCAAGTCTAACATTCTTTTTAATTTGTTTACACTATCATTTATAAAGTAGTTAAAAGATATATTTGAGATTGGTTTTGAAATCACTAAAATCCCTCGTAATAGCTTGGATCGATAACTGTGTTCAAAGAGCTGAGTATGATTAGTTTGTTTGCAGCTTTATTGGTTTGACAAAAAGGGAAAGGGTAATAGGTATGTCCTACTTCCAAAATCCCTCACAATAAGTTGGATCGATGATTGTCAATACTGGAAATGGATTCCCGGGGAGATCTATGGGTATGTTTTTTTTTTTTTTTTTTACTATCATTAAATATCGTTCTAGTTTCTATACTTTAAAATATCAATTTTTGAATGTGACAAAATATCTCGATTTACACATATACATATTTACAGTGTTTACGTCAATTTAATTTGTTATAAATTTATAAGTTAGTTTACTTAGTTTGTTATATTTGAAAGCAGTCTATAGTTCTTCCCCTTTCAATAAATTTAAGTTTTAGTCCATAGAACTTGCTTAGGGTTGATTTTATCTAAGTTTATGTATAAACAACTTTACCATAAATTTAGAAAATACTTACTCACGTCAGCGACTAAATTTAAGATTTATTATGGAAAGGTATGGATTAAAATTAAACAAAATTTAAATTAGAAGGACAAAACTGGAATTTTAACCTTAAATATATCTCATTCTGTCCTTCTATTTGTTTTCTTTTTATTTTCTTAACTTACTATTTTGTTTTTTTTTTTTTTCTTGTGTGTTGAAAAACAAAATCTTGAACAATGGAAGGTATATACTGACATAAAAAAAAATGAAAAAAATAAAACATAAAAGATTAGGTTTAAATTTGAATGAACTTTAATTTTTATTGTTTATTTAGTAATTAAAATTAAAAAGGTTGATTGGATGTAATTTTGAGGAATGGTTTTATAGAGAACAAATAGATTAAATTACTGACATTGGAATAGGAATGAGAAAAAGTTAGTTTTTATGATTTAATAAATGAAAATTTTCAAAAACACTAAATTTTACAAAATATTTATAACCTATAGCAAATTCTATTATTGATAGTCACTGATATACTATAGTGATAGAAGATTAGAATTCAAAATTTTGCTATAACTTGTAGATATTTTAATTTATTTTGCTATTTTTAAAAATGTCCCTTTAATAAAATCACTTTCTAAAATTTCATAAGACTAAATTGAACTTAACCTTTTACTGAAAGACCAAATTTACGATTTTAAAAAAATGTCGTGAAAAGAAAATTTTCGTTCTACAAACAATTAGATGGAATGCATAGAAATATACTAAGTTGTTTATTTGTTGATGATGGTGTAAAAAAAAAAAAAAATGGCAGTAAGAAAATTGACGTGGCAGAGCTTATAAAAAGTATGTTGGTTGGATATAAGTGGAAAGTTGAAGCAATACGCACTATCATCTGGAGTTCTATATGAAGTAATATGTCATGTTGGCGTTACACCTTGTGCCTCTGGATGGCAAGAACCTGTTACCTTTGCAATCACCCTCCCAGATGGAAAAAAAATTGAGATCAAAGAAAGCATACAATGTAAGCCACGAGATGTTTGGATTACGATCAAGATTGGGGAATTCAAGGTCGACAAACATGATTGTAACAGTGATAAGGAGTACGAGTTTAGAGTGTATAGTCACAGCAGTCAATGGAAGACGGGGTTGAAATTTAAAGGTTTCGAAATTCGACCAAAACAATCGAGTTGCGGATGCTAAAAATATAAATCACTAAATCTCTCTCACTCCCACTCCCACTACCCTACTTGTAGTTTGGTTTAAATAAATGAGAGAGGTATACTACACTCTACTTTGTTTTCTAATATAAACAACAATATCACTATGTAATACAGTAATAGTGTTCAATAAAAAAAAAATTAATAAAGATATATATATATATAATATAGAAAACTACCTTCTACTTAGTATATTTATATATATATATATATATA

Coding sequence (CDS)

ATGGTTTGCATAGAGACAGAAGCAAGAGAGAGCTTGCAAATACAGGAGAGTTATGGTCATTGTCTTACTTATATTTTGCCAAAAACTCTAAATGTCACGGTGCATTGGCCTACCTATGCTAAGCTCTATCAACAACTTATTGCTGGAATCACCCTAAACAAGGGAACTAAGCTTTATTGGTTTGACAAAAAGGGAAAGGGTAATAGGTATGTCCTACTTCCAAAATCCCTCACAATAAGTTGGATCGATGATTGTCAATACTGGAAATGGATTCCCGGGGAGATCTATGGTAAGAAAATTGACGTGGCAGAGCTTATAAAAAGTATGTTGGTTGGATATAAGTGGAAAGTTGAAGCAATACGCACTATCATCTGGAGTTCTATATGA

Protein sequence

MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIKSMLVGYKWKVEAIRTIIWSSI

Homology

[Close]

BLAST of Cucsat.G8436 vs. ExPASy Swiss-Prot
Match: C0HJV2 (Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 8.1e-16
Identity = 42/89 (47.19%), Postives = 58/89 (65.17%), Query Frame = 0


Query: 19  GHCLTYILPKTLNVTVH-WPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVLLPKSL 78
           GH L  IL K L+V V+  P++ KLY Q+ AGI LN  TK YWFDK  + N ++L  + L
Sbjct: 11  GHNLEAIL-KGLDVDVYSVPSFIKLYDQVTAGIFLNNRTKRYWFDKNAESNCFMLYARDL 70

Query: 79  TISWIDDCQYWKWIPGEIYGKKIDVAELI 107
            I+W  D +YW+W P + +G  ++VAELI
Sbjct: 71  LITWSQDKRYWRWNPFQEHGNTLEVAELI 98

[Close]

BLAST of Cucsat.G8436 vs. ExPASy Swiss-Prot
Match: P0DSP5 (Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.8e-05
Identity = 18/68 (26.47%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 63  KKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIKSMLVGYKWKVEA--I 122
           +K     ++L P++ T++W DD +YW W P +  G +++ A+L +      +W V    +
Sbjct: 4   EKLSSTHFLLFPRAATLTWSDDTRYWSWNPVDFCGYQLEEAQLSRVSWFDCRWTVNTTDL 63

Query: 123 RTIIWSSI 129
           +T +W ++
Sbjct: 64  KTNVWYNV 71

[Close]

BLAST of Cucsat.G8436 vs. NCBI nr
Match: XP_004135559.1 (lectin [Cucumis sativus] >KGN65960.1 hypothetical protein Csa_023237 [Cucumis sativus])

HSP 1 Score: 228 bits (580), Expect = 2.89e-73
Identity = 105/107 (98.13%), Postives = 106/107 (99.07%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKSLTI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. NCBI nr
Match: AAM89256.1 (26 kDa phloem protein [Cucumis sativus])

HSP 1 Score: 227 bits (578), Expect = 5.81e-73
Identity = 104/107 (97.20%), Postives = 106/107 (99.07%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKT+NVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKSLTI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. NCBI nr
Match: CDK12646.1 (phloem lectin [Cucumis sativus])

HSP 1 Score: 225 bits (573), Expect = 3.35e-72
Identity = 104/107 (97.20%), Postives = 105/107 (98.13%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKS TI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. NCBI nr
Match: AAM82557.1 (26 kDa phloem lectin, partial [Cucumis sativus])

HSP 1 Score: 208 bits (530), Expect = 7.85e-66
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 0


Query: 13  QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL 72
           QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL
Sbjct: 1   QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL 60

Query: 73  LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK
Sbjct: 61  LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 95

[Close]

BLAST of Cucsat.G8436 vs. NCBI nr
Match: NP_001284457.1 (protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem lectin [Cucumis melo])

HSP 1 Score: 176 bits (446), Expect = 6.65e-53
Identity = 79/118 (66.95%), Postives = 98/118 (83.05%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVC+ETEARE+LQI+E +GHCL+YILP T N  VHWP+Y+KLYQQL  GITLN GTK+YW
Sbjct: 1   MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIKSMLVGYKWKVE 118
           FDKKGKGN Y LLPKSL+I+WIDD +YWKWI  E+ GKK++VAELI+   +  + K++
Sbjct: 61  FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIK 118

[Close]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL
Match: A0A0A0LVN2 (Phloem protein OS=Cucumis sativus OX=3659 GN=Csa_1G542510 PE=4 SV=1)

HSP 1 Score: 228 bits (580), Expect = 1.40e-73
Identity = 105/107 (98.13%), Postives = 106/107 (99.07%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKSLTI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL
Match: Q8LK13 (26 kDa phloem protein OS=Cucumis sativus OX=3659 GN=PP2 PE=2 SV=1)

HSP 1 Score: 227 bits (578), Expect = 2.81e-73
Identity = 104/107 (97.20%), Postives = 106/107 (99.07%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKT+NVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKSLTI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL
Match: W0ZFB6 (Phloem lectin OS=Cucumis sativus OX=3659 GN=pp2 PE=2 SV=1)

HSP 1 Score: 225 bits (573), Expect = 1.62e-72
Identity = 104/107 (97.20%), Postives = 105/107 (98.13%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           FDKKGKGNRYVLLPKS TI WIDDCQYWKWIPGEIYGKKIDVA+LIK
Sbjct: 61  FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYGKKIDVAQLIK 107

[Close]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL
Match: Q8LK68 (26 kDa phloem lectin (Fragment) OS=Cucumis sativus OX=3659 GN=Lec26 PE=2 SV=1)

HSP 1 Score: 208 bits (530), Expect = 3.80e-66
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 0


Query: 13  QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL 72
           QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL
Sbjct: 1   QIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYWFDKKGKGNRYVL 60

Query: 73  LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 107
           LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK
Sbjct: 61  LPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIK 95

[Close]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL
Match: Q8LK97 (26 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1)

HSP 1 Score: 176 bits (446), Expect = 3.22e-53
Identity = 79/118 (66.95%), Postives = 98/118 (83.05%), Query Frame = 0


Query: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60
           MVC+ETEARE+LQI+E +GHCL+YILP T N  VHWP+Y+KLYQQL  GITLN GTK+YW
Sbjct: 1   MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61  FDKKGKGNRYVLLPKSLTISWIDDCQYWKWIPGEIYGKKIDVAELIKSMLVGYKWKVE 118
           FDKKGKGN Y LLPKSL+I+WIDD +YWKWI  E+ GKK++VAELI+   +  + K++
Sbjct: 61  FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIK 118

The following BLAST results are available for this feature:

BLAST of Cucsat.G8436 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HJV2	8.1e-16	47.19	Lectin OS=Luffa acutangula OX=56866 PE=1 SV=1	[more]
P0DSP5	3.8e-05	26.47	Lectin OS=Coccinia grandis OX=387127 PE=1 SV=1	[more]

BLAST of Cucsat.G8436 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135559.1	2.89e-73	98.13	lectin [Cucumis sativus] >KGN65960.1 hypothetical protein Csa_023237 [Cucumis sa...	[more]
AAM89256.1	5.81e-73	97.20	26 kDa phloem protein [Cucumis sativus]	[more]
CDK12646.1	3.35e-72	97.20	phloem lectin [Cucumis sativus]	[more]
AAM82557.1	7.85e-66	100.00	26 kDa phloem lectin, partial [Cucumis sativus]	[more]
NP_001284457.1	6.65e-53	66.95	protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo] >AAM74921.1 26 kDa phloem l...	[more]

BLAST of Cucsat.G8436 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVN2	1.40e-73	98.13	Phloem protein OS=Cucumis sativus OX=3659 GN=Csa_1G542510 PE=4 SV=1	[more]
Q8LK13	2.81e-73	97.20	26 kDa phloem protein OS=Cucumis sativus OX=3659 GN=PP2 PE=2 SV=1	[more]
W0ZFB6	1.62e-72	97.20	Phloem lectin OS=Cucumis sativus OX=3659 GN=pp2 PE=2 SV=1	[more]
Q8LK68	3.80e-66	100.00	26 kDa phloem lectin (Fragment) OS=Cucumis sativus OX=3659 GN=Lec26 PE=2 SV=1	[more]
Q8LK97	3.22e-53	66.95	26 kDa phloem lectin OS=Cucumis melo OX=3656 GN=Lec26 PE=2 SV=1	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G8436.T1	Cucsat.G8436.T1	mRNA
Cucsat.G8436.T2	Cucsat.G8436.T2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0030246	carbohydrate binding