Cucsat.G8428 (gene) Cucumber (B10) v3

BLAST of Cucsat.G8428 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.7e-140
Identity = 257/544 (47.24%), Postives = 359/544 (65.99%), Query Frame = 0

Query: 11  SLIAFIVICS---SFSLFNAASS--DKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S  +F  +C     F  FN  +S  +  E FL+C   + P++++  KL+YTQNN  Y SV
Sbjct: 4   STFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSV 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +I N RF++  TPKP++I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+RSI +DV  +TAWV++ ATLGE+YY + EK+  L+   G CPTVC GG
Sbjct: 124 QVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           +  GGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+VA
Sbjct: 184 HFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDG 310
           WK++LV VP +  F         + + L+++WQ + YK +K++      I   +T     
Sbjct: 244 WKIRLVAVPKSTMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGK 303

Query: 311 KVKPVAIFYTS-FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AANDFTVG 370
               +  +++S FLG  +  V ++ + FP+LG+KK +C++ SW+++++      N  T  
Sbjct: 304 NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDN 363

Query: 371 EPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPY 430
              E LL+RSA      +   KIK DYVK+P+P+     I  ++   +DIG       PY
Sbjct: 364 FNKEILLDRSA----GQNGAFKIKLDYVKKPIPESVFVQILEKL-YEEDIGAGMYALYPY 423

Query: 431 GGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSP 490
           GG M EISES I F HRAG L+++ Y+  WE    +   +HLNWIR IY++M P+VSK+P
Sbjct: 424 GGIMDEISESAIPFPHRAGILYELWYICSWE--KQEDNEKHLNWIRNIYNFMTPYVSKNP 483

Query: 491 RSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHE 546
           R AY+NYRDLDIG N  K  N   N+ QA  WG KY+G NF+RLV++KT VDP NFFR+E
Sbjct: 484 RLAYLNYRDLDIGINDPKNPN---NYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNE 537

BLAST of Cucsat.G8428 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-139
Identity = 260/551 (47.19%), Postives = 360/551 (65.34%), Query Frame = 0

Query: 8   IHLSLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 67
           ++ S  +F  +C     F       + ++  E FL+C   H P++ +  KL+YTQ++  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLY 60

Query: 68  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 127
            S+LN +I+N RF +  TPKP++I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+S
Sbjct: 61  MSILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMS 120

Query: 128 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 187
           Y++ +PF+VVDL N+ SI +DV  +TAWV++ ATLGE+YY I EK+  L+FPGG CPTV 
Sbjct: 121 YISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVG 180

Query: 188 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 247
            GG+ SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 248 VVAWKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTS 307
           + AWK+KLV VP+  T  S  +  E    + L ++WQ + YK +K++      I   +T 
Sbjct: 241 IAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITD 300

Query: 308 EVDGKVKPVAIFYTS--FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AAN 367
              GK K     Y S  F G  +  V ++ + FP+LG+KK +CKE SW+++ +      N
Sbjct: 301 N-HGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVN 360

Query: 368 DFTVGEPVEALLNRSALIPPITSKKV--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGI 427
             T     E LL+RSA       KK    IK DYVK+P+P+ A+  I  ++   +D+G  
Sbjct: 361 FNTANFKKEILLDRSA------GKKTAFSIKLDYVKKPIPETAMVKILEKL-YEEDVGAG 420

Query: 428 NVLFVPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMA 487
             +  PYGG M EISES I F HRAG ++++ Y   WE    +   +H+NW+R +Y++  
Sbjct: 421 MYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTT 480

Query: 488 PFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDP 546
           P+VS++PR AY+NYRDLD+G  +    N   N+ QA  WG KY+G NFNRLV++KTKVDP
Sbjct: 481 PYVSQNPRLAYLNYRDLDLGKTNHASPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDP 538

BLAST of Cucsat.G8428 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.6e-137
Identity = 252/546 (46.15%), Postives = 356/546 (65.20%), Query Frame = 0

Query: 11  SLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S   F  +C     F       + ++  E FL+CL  + P + + AKL+YTQ++  Y S+
Sbjct: 4   STFCFWYVCKIIFFFLSFNIQISIANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSI 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +++N RF++  TPKP++I TP N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+ S+ +DV  +TAWV+S ATLGE+YY I E +  L+FP G CPTV  GG
Sbjct: 124 QVPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           + SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 184 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD 310
           WK++LV VP+  T  S  +  E  + + L+++WQ + Y  EK +      I   +T    
Sbjct: 244 WKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-Q 303

Query: 311 GKVKPV--AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AANDFT 370
           GK K    + F + F G  +  V ++ + FP+LG+KK +CK+ SW+++++      N  T
Sbjct: 304 GKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNT 363

Query: 371 VGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 430
                E LL+RS            IK DYVK+P+P+ A+  I  ++   +D+G    +F 
Sbjct: 364 TNFKKEILLDRSG----GRKAAFSIKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFY 423

Query: 431 PYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 490
           PYGG M EISES I F HRAG  ++I Y+  WE    +   +H+NWIR +Y++  P+VS+
Sbjct: 424 PYGGIMDEISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQ 483

Query: 491 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 546
           +PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR
Sbjct: 484 NPRMAYLNYRDLDLGKTNFESPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFR 538

BLAST of Cucsat.G8428 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 2.1e-137
Identity = 252/546 (46.15%), Postives = 357/546 (65.38%), Query Frame = 0

Query: 11  SLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSV 70
           S   F  +C     F       + ++  E FL+C   + P + + AKL+YTQ++  Y S+
Sbjct: 4   STFCFWYVCKIIFFFLSFNIQISIANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSI 63

Query: 71  LNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVA 130
           LN +I+N RF++  TPKP++IITP N+SHIQ  ++CSK  GLQIR RSGGHD EG+SY++
Sbjct: 64  LNSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS 123

Query: 131 YLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGG 190
            +PF++VDL N+ S+ +DV  +TAWV++ ATLGE+YY I E +  L+FP G CPTV  GG
Sbjct: 124 QVPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGG 183

Query: 191 YLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVA 250
           + SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ A
Sbjct: 184 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 243

Query: 251 WKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVD 310
           WK++LV VP+  T  S  +  E  + + L+++WQ + Y  EK +      I   +T    
Sbjct: 244 WKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDN-Q 303

Query: 311 GKVKPV--AIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---AANDFT 370
           GK K    + F + F G  +  V ++ + FP+LG+KK +CK+ SW+++++      N  T
Sbjct: 304 GKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNT 363

Query: 371 VGEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 430
                E LL+RS            IK DYVK+P+P+ A+  I  ++   +D+G    +F 
Sbjct: 364 TYFKKEILLDRSG----GRKAAFSIKLDYVKKPIPETAMVTILEKL-YEEDVGVGMFVFY 423

Query: 431 PYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 490
           PYGG M EISES I F HRAG +++I Y+  WE    +   +H+NWIR +Y++  P+VS+
Sbjct: 424 PYGGIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQ 483

Query: 491 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 546
           +PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTKVDP NFFR
Sbjct: 484 NPRMAYLNYRDLDLGKTNFESPN---NYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFR 538

BLAST of Cucsat.G8428 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 6.2e-137
Identity = 257/553 (46.47%), Postives = 363/553 (65.64%), Query Frame = 0

Query: 8   IHLSLIAFIVICSSFSLF-----NAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 67
           ++ S  +F  +C     F       + ++  E FL+C   + P++ +  K IYTQ++  Y
Sbjct: 1   MNCSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLY 60

Query: 68  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 127
            SVLN +I+N RF++  TPKP++I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGLS
Sbjct: 61  MSVLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLS 120

Query: 128 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 187
           Y++ +PF +VDL N+ ++ VD+  +TAWV++ ATLGE+YY I E +   +FPGG CPTV 
Sbjct: 121 YISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVG 180

Query: 188 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 247
            GG+ SGGGYG L+R YGLAADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI
Sbjct: 181 VGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGI 240

Query: 248 VVAWKVKLVPVPATVTFCSSSRTFE-EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTS 307
           + AWK+KLV VP+  T  S  +  E    + L ++WQ + YK +K++   +LT   +  +
Sbjct: 241 IAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDL---MLTTHFRTRN 300

Query: 308 EVD--GKVKPVAIFYTS--FLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVI---A 367
             D  GK K     Y S  FLG  +  V ++ + FP+LG+KK +CKE SW+++ +     
Sbjct: 301 ITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGV 360

Query: 368 ANDFTVGEPVEALLNRSALIPPITSKKV--KIKSDYVKEPMPKVAIEGIWNRVNNSQDIG 427
            N  T     E LL+RSA       KK    IK DYVK+ +P+ A+  I  ++   +   
Sbjct: 361 VNYNTANFKKEILLDRSA------GKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGV 420

Query: 428 GINVLFVPYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSY 487
           G+ VL+ PYGG M EISES I F HRAG ++++ Y   WE    +   +H+NW+R +Y++
Sbjct: 421 GMYVLY-PYGGIMDEISESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNF 480

Query: 488 MAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKV 546
             P+VS++PR AY+NYRDLD+G  + +  N   N+ QA  WG KY+G NFNRLV++KTK 
Sbjct: 481 TTPYVSQNPRLAYLNYRDLDLGKTNPESPN---NYTQARIWGEKYFGKNFNRLVKVKTKA 538

BLAST of Cucsat.G8428 vs. NCBI nr
Match: XP_004135739.1 (berberine bridge enzyme-like 28 [Cucumis sativus])

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60
           MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS
Sbjct: 1   MKKSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNS 60

Query: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120
           SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG
Sbjct: 61  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180
           LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT
Sbjct: 121 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 180

Query: 181 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 300
           GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT
Sbjct: 241 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 300

Query: 301 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV 360
           SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV
Sbjct: 301 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV 360

Query: 361 GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP 420
           GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP
Sbjct: 361 GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVP 420

Query: 421 YGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS 480
           YGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS
Sbjct: 421 YGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKS 480

Query: 481 PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH 540
           PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH
Sbjct: 481 PRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRH 540

Query: 541 EQSIPIALSQEDNLVKLV 558
           EQSIPIALSQEDNLVKLV
Sbjct: 541 EQSIPIALSQEDNLVKLV 558

BLAST of Cucsat.G8428 vs. NCBI nr
Match: KAE8653320.1 (hypothetical protein Csa_023331 [Cucumis sativus])

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 531/531 (100.00%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 28  ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 87
           ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII
Sbjct: 4   ASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIRNHRFSTPNTPKPILII 63

Query: 88  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 147
           TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR
Sbjct: 64  TPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIVVDLINLRSITVDVKRR 123

Query: 148 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 207
           TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI
Sbjct: 124 TAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGGYGLLLRKYGLAADNVI 183

Query: 208 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 267
           DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE
Sbjct: 184 DAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTFCSSSRTFE 243

Query: 268 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 327
           EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI
Sbjct: 244 EDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVAIFYTSFLGKANKAVKI 303

Query: 328 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 387
           LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD
Sbjct: 304 LKEKFPQLGLKKEECKEASWVESVVIAANDFTVGEPVEALLNRSALIPPITSKKVKIKSD 363

Query: 388 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 447
           YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY
Sbjct: 364 YVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISESEISFSHRAGNLFKIAY 423

Query: 448 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 507
           LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH
Sbjct: 424 LTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRDLDIGSNSDKYGNIVTNH 483

Query: 508 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 558
           DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV
Sbjct: 484 DQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQEDNLVKLV 534

BLAST of Cucsat.G8428 vs. NCBI nr
Match: XP_011659204.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653317.1 hypothetical protein Csa_023357 [Cucumis sativus])

HSP 1 Score: 752 bits (1941), Expect = 4.58e-268
Identity = 376/541 (69.50%), Postives = 446/541 (82.44%), Query Frame = 0

Query: 13  IAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 72
           +AFI +CSS S++ +ASS   HEEFLQCLLHHSPHS SIAKL YT  N+SYSSVLN SIR
Sbjct: 11  LAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTSYSSVLNFSIR 70

Query: 73  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFS PNTPKP+ IITP+NISH+QAAVICSKS GLQIRIRSGGHDFEGLSYVAY PFI+
Sbjct: 71  NLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGLSYVAYHPFII 130

Query: 133 VDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLI+L S+T++VK+ TAWVQS ATLGELYYRIAEKS TL FP G  PTV  GG+ SGGG
Sbjct: 131 VDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYGLAADNVIDAYLVDANG FHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYLVDANGVFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVA 312
           PVPATVT C+ SRT EE+AI L+ QWQYV  KL++++  G+  +GG+++++ D K+ P+A
Sbjct: 251 PVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISAQGD-KINPIA 310

Query: 313 IFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNR 372
           +F++ FLGKA++ + IL + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR
Sbjct: 311 LFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNR 370

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +    P+ +  +K+KSDYVKEP+PK  IE IW R+  SQDI G N++FVPYGGRMS+IS+
Sbjct: 371 T----PLATGNIKMKSDYVKEPIPKATIEEIWQRLE-SQDIEGANLVFVPYGGRMSQISD 430

Query: 433 SEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI FSHRAGNL+KI YLTGW +P ++ E  HLNWIR+IY YM PFVSKSPR+AYVNYRD
Sbjct: 431 SEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAYVNYRD 490

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 551
           LDIGSNS KYG   T++ +A  WGLKY+GNNFNR+V +K KVDPYNFFRHEQSIP+ L  
Sbjct: 491 LDIGSNS-KYGK--TSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLLKH 542

BLAST of Cucsat.G8428 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 751 bits (1940), Expect = 6.74e-268
Identity = 382/546 (69.96%), Postives = 445/546 (81.50%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSD--KHEEFLQCLLHHSPHSSSIAKLIYTQNNS 62
           K SP I LSL AFI++CSS SL +A SS   K+EEFLQCLLHHSP++ SI  L+Y   NS
Sbjct: 2   KFSPLIPLSL-AFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 61

Query: 63  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 122
           SYSS+LN SIRN RFSTP  PKP+LIITPSNISHIQAAV+CSKS GLQIRIRSGGHDFEG
Sbjct: 62  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 121

Query: 123 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 182
           LSYVAY PFIVVDLINL S+ VDV++ TAWVQS ATLGELYYRIAEKS TL FP G CPT
Sbjct: 122 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 181

Query: 183 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 242
           V  GG+ SGGGYG LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 182 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 241

Query: 243 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 302
           GIVVAWKVKLVPVPATVT C+++RT++E AI LI QWQYV  KL++++   ++  GG+++
Sbjct: 242 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 301

Query: 303 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV 362
           ++  GK+ P A+F+T FLGKA++ + ILK KFP+L L KE+CKE SW+ES V  AN   +
Sbjct: 302 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 361

Query: 363 GE-PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 422
            + P+EA LNR+    P+ +   KIKSDYVKEP+ K AIEGIW R+  SQDI G+N+  +
Sbjct: 362 DDQPLEAFLNRT----PLANGSFKIKSDYVKEPITKAAIEGIWQRLK-SQDIEGVNLGLI 421

Query: 423 PYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 482
           PYGGRMS+ISESEI F HR GNL+KI Y+ GWE+  +D E RHLNWIREIYSYM PFVSK
Sbjct: 422 PYGGRMSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSK 481

Query: 483 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 542
           SPR+AYVNYRDLDIGSN DKYG   T++ +A  WG KY+GNNFNRLV +K +VDPY+FFR
Sbjct: 482 SPRAAYVNYRDLDIGSN-DKYGK--TSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFR 537

Query: 543 HEQSIP 545
           HEQSIP
Sbjct: 542 HEQSIP 537

BLAST of Cucsat.G8428 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 749 bits (1935), Expect = 4.03e-267
Identity = 378/548 (68.98%), Postives = 448/548 (81.75%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSD--KHEEFLQCLLHHSPHSSSIAKLIYTQNNS 62
           K SP I LSL AFI++CSS SL +A SS   KHEEFLQCLLHHSP++ SI KL+Y   NS
Sbjct: 2   KFSPLILLSL-AFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 61

Query: 63  SYSSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEG 122
           SYSS+LN   RN RFSTPN P P+LIITP+NISHIQAA+ICSKS GLQIRIRSGGHDFEG
Sbjct: 62  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 121

Query: 123 LSYVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPT 182
           LSYVAY+PFIVVDLINLRS+TVD ++ +AWVQS ATLGELYYRIAEKS TL FP G CPT
Sbjct: 122 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 181

Query: 183 VCFGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 242
           V  GG+ SGGGYG+LLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 182 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 241

Query: 243 GIVVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMT 302
           G+VVAWKVKLVPVP TVT C++ RT EE AI LI QWQYVG KLE+++   +   GG+ +
Sbjct: 242 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 301

Query: 303 SEVDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV 362
           ++  GKV P A+F++ FLGKA++ V ILK+KFP+LGL KE+CKE SW+ S V AAN F +
Sbjct: 302 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 361

Query: 363 -GEPVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFV 422
             +P+EALLNR+    P+ +  +KIKSDYVKE + KV IEGIW R+  SQDI G+ +  +
Sbjct: 362 EDQPLEALLNRT----PLANGSLKIKSDYVKELITKVGIEGIWERLK-SQDIEGVTLALI 421

Query: 423 PYGGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSK 482
           PYGGRM +IS+SEI FSHRAG  ++I Y+ GW++  +D E RH+NWIREIYSYM PFVSK
Sbjct: 422 PYGGRMGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSK 481

Query: 483 SPRSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFR 542
           SPR+AYVNYRD DIGSN+ KYG +  ++ +A  WGLKY+GNNFNRLV++KTKVDP NFFR
Sbjct: 482 SPRAAYVNYRDHDIGSNN-KYGKV--SYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFR 540

Query: 543 HEQSIPIA 547
           HEQSIP++
Sbjct: 542 HEQSIPLS 540

BLAST of Cucsat.G8428 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 746 bits (1925), Expect = 6.00e-266
Identity = 371/540 (68.70%), Postives = 446/540 (82.59%), Query Frame = 0

Query: 13  IAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S++ +ASS   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKP+LIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T++V++ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVA 312
           PVPATVT C+ SRT EE+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIA 310

Query: 313 IFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNR 372
           +F++ FLGKA++ + IL + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR
Sbjct: 311 LFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNR 370

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +    P+ +  +K+KSDYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISE
Sbjct: 371 T----PLANGNIKMKSDYVKEPISKATIKEVWQRLK-SQDIEGANLVFIPYGGRMSQISE 430

Query: 433 SEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI FSHRAGNL+KI YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRD
Sbjct: 431 SEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRD 490

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 550
           LDIGSNS KYG   T++ +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 491 LDIGSNS-KYGK--TSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of Cucsat.G8428 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 746 bits (1925), Expect = 6.00e-266
Identity = 371/540 (68.70%), Postives = 446/540 (82.59%), Query Frame = 0

Query: 13  IAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYSSVLNLSIR 72
           +AFIV+CSS S++ +ASS   HEEFL CLLHHSP S SI KL+YT  N+SYS VLN SIR
Sbjct: 11  LAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTSYSPVLNFSIR 70

Query: 73  NHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSYVAYLPFIV 132
           N RFSTPNTPKP+LIITPSNISH+QAAVICSKS G+QIRIRSGGHDFEGLSYVAY PF+V
Sbjct: 71  NLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGLSYVAYHPFVV 130

Query: 133 VDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCFGGYLSGGG 192
           VDLINL S+T++V++ TAWVQS ATLGELYYRIAEKS TL FP G CPTV  GG+ SGGG
Sbjct: 131 VDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTVGVGGHFSGGG 190

Query: 193 YGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 252
           +G LLRKYGLAADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFGIVVAWKVKLV
Sbjct: 191 FGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250

Query: 253 PVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEVDGKVKPVA 312
           PVPATVT C+ SRT EE+AI LI QWQYV  KL++++  G+  +GG++ ++ D K+ P+A
Sbjct: 251 PVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVAQGD-KINPIA 310

Query: 313 IFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GEPVEALLNR 372
           +F++ FLGKA++ + IL + FPQLGL KEECKE SW+ESVV   N   +  +P+E LLNR
Sbjct: 311 LFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIEDQPLEVLLNR 370

Query: 373 SALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYGGRMSEISE 432
           +    P+ +  +K+KSDYVKEP+ K  I+ +W R+  SQDI G N++F+PYGGRMS+ISE
Sbjct: 371 T----PLANGNIKMKSDYVKEPISKATIKEVWQRLK-SQDIEGANLVFIPYGGRMSQISE 430

Query: 433 SEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPRSAYVNYRD 492
           SEI FSHRAGNL+KI YLTGW +P ++ E  HLNWIR+IYSYM PFV+KSPR+AYVNYRD
Sbjct: 431 SEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAYVNYRD 490

Query: 493 LDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQSIPIALSQ 550
           LDIGSNS KYG   T++ +A  WG KY+G+NF+RLV +K +VDPYNFFRHEQSIP+ L +
Sbjct: 491 LDIGSNS-KYGK--TSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPVLLKR 541

BLAST of Cucsat.G8428 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 737 bits (1902), Expect = 3.02e-262
Identity = 376/546 (68.86%), Postives = 443/546 (81.14%), Query Frame = 0

Query: 5   SPSIHLSLIAFIVICSSFSLFNAASSD-KHEEFLQCLLHHSPHSSSIAKLIYTQNNSSYS 64
           SP + LSL+ FIV+CSS SL+ A SS  KHEEFLQCLLHHSP +SSI KL++T  NSS+S
Sbjct: 17  SPLVPLSLV-FIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSSFS 76

Query: 65  SVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLSY 124
           ++ N   RN RFSTPNTPKP+LIITPSNISHIQAAVICSKS GLQIRIRSGGHDFEGLSY
Sbjct: 77  TLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 136

Query: 125 VAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVCF 184
           VAY  FIVVDLINLRS+TVDV++ TAWVQS ATLGELYY+I +KS TL FP G CPTV  
Sbjct: 137 VAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTVGV 196

Query: 185 GGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIV 244
           GG+ SGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG+V
Sbjct: 197 GGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGVV 256

Query: 245 VAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSEV 304
           VAWKVKLVPVPA+VT CS+ R+ EE+AI LI +WQYV  KLE+++   +L  GG+++++ 
Sbjct: 257 VAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSAQG 316

Query: 305 DGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTV-GE 364
            GK+ P+A+F+  FLG  N+ + IL + FP+LG  KEECKE SW+ES   AAN F +  +
Sbjct: 317 GGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIEDQ 376

Query: 365 PVEALLNRSALIPPIT-SKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPY 424
           P+EALLNR+    PI      K+KSDYVKEPM K AIEGIW R+  SQDI G+ +  +PY
Sbjct: 377 PLEALLNRT----PINHGSSFKLKSDYVKEPMTKTAIEGIWKRLE-SQDIEGVTLALIPY 436

Query: 425 GGRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSP 484
           GGRMS ISES I FSHRAG L++I Y+ GW++  ++ E RHLNWIREIYSYM PFVSKSP
Sbjct: 437 GGRMSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSP 496

Query: 485 RSAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHE 544
           R+AYVNYRDLDIGSN+ KYG I  N+ QA  +G KY+GNNFNRLV++KTKVDPYNFF HE
Sbjct: 497 RTAYVNYRDLDIGSNN-KYGKI--NYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHE 553

Query: 545 QSIPIA 547
           QSIP++
Sbjct: 557 QSIPLS 553

BLAST of Cucsat.G8428 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 732 bits (1890), Expect = 2.41e-260
Identity = 372/556 (66.91%), Postives = 446/556 (80.22%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 62
           KSSP   LSL  FIV+CSS SL+ A SS+++++FLQCLLHHSP + SI KL+YT  N+SY
Sbjct: 2   KSSPLTLLSL-TFIVLCSS-SLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASY 61

Query: 63  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 122
           SS+LN SI+N RFSTP TPKP+ IITPSNI HIQAA+ICSKS GLQIRIRSGGHD EGLS
Sbjct: 62  SSLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLS 121

Query: 123 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 182
           YVAY+PFIVVDLINL  + VDV++ TAWVQS A+LGELYYRIAEKS TL FP G CPTV 
Sbjct: 122 YVAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVG 181

Query: 183 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 242
            GG  SGGGYG+L+RKYGLAADNV+DAYLVDANGEF DRE MGE+LFWAIRGGGGGSFG+
Sbjct: 182 VGGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGV 241

Query: 243 VVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSE 302
           VVAWKVKLVPVPATVT C++S++  + AINLI +WQYV  KL++N+  G++  GG     
Sbjct: 242 VVAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAGG----- 301

Query: 303 VDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGE 362
             GK  P+AIF++ FLGKA++ V IL   FP+LGL KE+C E SW+E+ +IAAN F  GE
Sbjct: 302 --GKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGE 361

Query: 363 PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 422
            +EALLNRSALIPPI S + K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 362 VLEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 421

Query: 423 GRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPR 482
           G+MSEISESEI F HRAGN ++I Y+ GW++  +  E RHLNWIRE+YSYM PFVSKSPR
Sbjct: 422 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPR 481

Query: 483 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 542
           + YVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RLV +KT+VDPY+FFRHEQ
Sbjct: 482 AVYVNCRDLDIGSN-DMYGR--TSYKRARVWGLKYFGNNFDRLVHVKTEVDPYDFFRHEQ 541

Query: 543 SIPIALSQEDNLVKLV 558
           SIP  LS      K++
Sbjct: 542 SIP-TLSPTKEFSKII 543

BLAST of Cucsat.G8428 vs. ExPASy TrEMBL
Match: A0A6J1EMV2 (berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE=3 SV=1)

HSP 1 Score: 732 bits (1889), Expect = 3.43e-260
Identity = 373/556 (67.09%), Postives = 447/556 (80.40%), Query Frame = 0

Query: 3   KSSPSIHLSLIAFIVICSSFSLFNAASSDKHEEFLQCLLHHSPHSSSIAKLIYTQNNSSY 62
           KSSP   LSL  FIV+CSS SL+ A SS+++++FLQCLLHHSP + SI KL+YT  N+SY
Sbjct: 2   KSSPLTLLSL-TFIVLCSS-SLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASY 61

Query: 63  SSVLNLSIRNHRFSTPNTPKPILIITPSNISHIQAAVICSKSQGLQIRIRSGGHDFEGLS 122
           SS+LN SI+N RFSTP TPKP+ IITPSNI HIQAA+ICSKS GLQIRIRSGGHD EGLS
Sbjct: 62  SSLLNFSIKNLRFSTPKTPKPLAIITPSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLS 121

Query: 123 YVAYLPFIVVDLINLRSITVDVKRRTAWVQSAATLGELYYRIAEKSPTLTFPGGACPTVC 182
           YVA++PFIVVDLINL S+ VDV++ TAWVQS A+LGELYYRIAEKS TLTFP G CPTV 
Sbjct: 122 YVAHVPFIVVDLINLSSVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLTFPAGLCPTVG 181

Query: 183 FGGYLSGGGYGLLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 242
            GG  SGGGYG+L+RKYGLAADNV+DAYLVD NGEF DRE MGE+LFWAIRGGGGGSFG+
Sbjct: 182 VGGQFSGGGYGMLMRKYGLAADNVVDAYLVDGNGEFFDREGMGEELFWAIRGGGGGSFGV 241

Query: 243 VVAWKVKLVPVPATVTFCSSSRTFEEDAINLIHQWQYVGYKLEKNIAHGVLTIGGQMTSE 302
           VVAWKVKLVPVPATVT C++S++  + AI+LI +WQYV  KL++N+  G++  GG     
Sbjct: 242 VVAWKVKLVPVPATVTVCATSKSLVDGAISLIDKWQYVADKLDENLFLGIILAGG----- 301

Query: 303 VDGKVKPVAIFYTSFLGKANKAVKILKEKFPQLGLKKEECKEASWVESVVIAANDFTVGE 362
             GK  P+AIF++ FLGKA++ V IL   FP+LGL K +C E SW+E+ +IAAN F  GE
Sbjct: 302 --GKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGE 361

Query: 363 PVEALLNRSALIPPITSKKVKIKSDYVKEPMPKVAIEGIWNRVNNSQDIGGINVLFVPYG 422
            +EALLNRSALIPPI S K K KSDYVKEPM + AIEG+WNR + SQ+I   +++F+PYG
Sbjct: 362 VLEALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNR-SKSQEIERASLVFIPYG 421

Query: 423 GRMSEISESEISFSHRAGNLFKIAYLTGWEDPSMDVETRHLNWIREIYSYMAPFVSKSPR 482
           G+MSEISESEI F HRAGN ++I Y+ GW++  +  E RHLNWIRE+YSYMAPFVSKSPR
Sbjct: 422 GKMSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPR 481

Query: 483 SAYVNYRDLDIGSNSDKYGNIVTNHDQASSWGLKYYGNNFNRLVQIKTKVDPYNFFRHEQ 542
           +AYVN RDLDIGSN D YG   T++ +A  WGLKY+GNNF+RL  +KT+VDPY+FFRHEQ
Sbjct: 482 AAYVNCRDLDIGSN-DMYGR--TSYQRARVWGLKYFGNNFDRLAHVKTEVDPYDFFRHEQ 541

Query: 543 SIPIALSQEDNLVKLV 558
           SIP  LS      K+V
Sbjct: 542 SIP-TLSPTQEFSKIV 543