Cucsat.G8172 (gene) Cucumber (B10) v3

BLAST of Cucsat.G8172 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 8.2e-141
Identity = 251/493 (50.91%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           +YT  +  Y SIL S  QN RF +   T KPLVI+ PS+ SH+Q+T++CSK  GLQIR R
Sbjct: 52  VYTQHDQLYMSILNSTIQNLRFISD-TTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTR 111

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EG+SYIS +PFVVVDL N+ SI++D  + TAWV++GAT+GE+YY I EK+  L+
Sbjct: 112 SGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLS 171

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FP G CPTVG+GGHFSGGGYG LMR YGLAADN+IDA+LV+ +G V DR++MGEDLFWAI
Sbjct: 172 FPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAI 231

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIG 241
           RGGGG +FGI+  WK+KLV VP   T   + K+++     K+  +WQ +A + ++ L++ 
Sbjct: 232 RGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLM 291

Query: 242 INLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQS 301
            +    N T    +N T     F S++ G  D LV +MN + P LG+ K +CKE SWI +
Sbjct: 292 THFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDT 351

Query: 302 TLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEV 361
           T+  +G  N        EILL + +     ++ IK DYVK+PI + A   I ++L  ++V
Sbjct: 352 TIFYSGVVNFNTANFKKEILLDR-SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDV 411

Query: 362 ETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYS 421
                 L+PYGG M  IS S  PF HRA  +Y++ YT  W +Q    NE+H+NW+R  Y+
Sbjct: 412 GAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEKQED--NEKHINWVRSVYN 471

Query: 422 FMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDP 481
           F TP+VS +PR AY+NYRDLD+G  N     +Y +A IWG KYFG NF +LV+VKT VDP
Sbjct: 472 FTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 531

Query: 482 SNFFRHEQSIPPL 487
           +NFFR+EQSIPPL
Sbjct: 532 NNFFRNEQSIPPL 540

BLAST of Cucsat.G8172 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.1e-140
Identity = 253/493 (51.32%), Postives = 335/493 (67.95%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           +YT  N  Y S+L S   N RF T   T KPLVI+ PSHVSH+Q T++CSK  GLQIR R
Sbjct: 52  VYTQNNPLYMSVLNSTIHNLRF-TSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTR 111

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD+EG+SYIS +PFV+VDL N++SI++D  + TAWV++GAT+GE+YY + EK+  L+
Sbjct: 112 SGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLS 171

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
             AG CPTV  GGHF GGGYG LMR YGLAADN+IDA+LV+ +G V DR++MGEDLFWA+
Sbjct: 172 LAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWAL 231

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIG 241
           RGGG  SFGI+V WK++LV VP   T   + K ++     K+V +WQ +A + ++ LL+ 
Sbjct: 232 RGGGAESFGIIVAWKIRLVAVPK-STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLM 291

Query: 242 INLTGGNPTKGVKRNPTA---SFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQS 301
            +    N T    +N TA    F S++LG  D LV +MN + P LG+ K +C++ SWI +
Sbjct: 292 THFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDT 351

Query: 302 TLIAAGFTNGQP----LEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEV 361
            +  +G  N        EILL + +   N ++KIK DYVK+PI +  F  I ++L  +++
Sbjct: 352 IIFYSGVVNYDTDNFNKEILLDR-SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDI 411

Query: 362 ETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYS 421
                 L+PYGG M  IS S  PF HRA  LY++ Y   W +Q    NE+HLNWIR  Y+
Sbjct: 412 GAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYN 471

Query: 422 FMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDP 481
           FMTP+VS +PR AY+NYRDLDIG N+     +Y +A IWG KYFG NF +LV+VKT VDP
Sbjct: 472 FMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDP 531

Query: 482 SNFFRHEQSIPPL 487
           +NFFR+EQSIPPL
Sbjct: 532 NNFFRNEQSIPPL 539

BLAST of Cucsat.G8172 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.3e-140
Identity = 252/495 (50.91%), Postives = 332/495 (67.07%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           +YT  +  Y SIL S  QN RF T   T KPLVII P +VSH+Q T++CSK  GLQIR R
Sbjct: 52  VYTQHDQFYMSILNSTIQNLRF-TSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTR 111

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EG+SYIS +PFV+VDL N+ S+++D  + TAWV++GAT+GE+YY I E +  L+
Sbjct: 112 SGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLS 171

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FPAG CPTVG GGHFSGGGYG LMR YGLAADN+IDA+LV+ +G V DR++MGEDLFWAI
Sbjct: 172 FPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAI 231

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIG 241
           RGGGG +FGI+  WK++LV VP + T   + K+++     K+V +WQ +A   E++LL+ 
Sbjct: 232 RGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLF 291

Query: 242 INLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQS 301
            +    N T    +N T   + F S++ G  D LV +MN + P LG+ K +CK+ SWI +
Sbjct: 292 THFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDT 351

Query: 302 TLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEV 361
            +  +G  N        EILL + +     ++ IK DYVK+PI + A   I ++L  ++V
Sbjct: 352 IIFYSGVVNYNTTYFKKEILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDV 411

Query: 362 ETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYS 421
                  +PYGG M  IS S  PF HRA  +Y+I Y   W +Q    NE+H+NWIR  Y+
Sbjct: 412 GVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQED--NEKHINWIRNVYN 471

Query: 422 FMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDP 481
           F TP+VS +PR AY+NYRDLD+G  N     +Y +A IWG KYFG NF +LV+VKT VDP
Sbjct: 472 FTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 531

Query: 482 SNFFRHEQSIPPLTL 489
            NFFR+EQSIPPL L
Sbjct: 532 DNFFRNEQSIPPLPL 542

BLAST of Cucsat.G8172 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.5e-139
Identity = 252/495 (50.91%), Postives = 330/495 (66.67%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           +YT  +  Y SIL S  QN RF T   T KPLVI  P +VSH+Q T++CSK  GLQIR R
Sbjct: 52  VYTQHDQFYMSILNSTVQNLRF-TSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTR 111

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EG+SYIS +PFV+VDL N+ S+++D  + TAWV+SGAT+GE+YY I E +  L+
Sbjct: 112 SGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLS 171

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FPAG CPTVG GGHFSGGGYG LMR YGLAADN+IDA+LV+ +G V DR++MGEDLFWAI
Sbjct: 172 FPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAI 231

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIG 241
           RGGGG +FGI+  WK++LV VP + T   + K+++     K+V +WQ +A   E++LL+ 
Sbjct: 232 RGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLF 291

Query: 242 INLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQS 301
            +    N T    +N T   + F S++ G  D LV +MN + P LG+ K +CK+ SWI +
Sbjct: 292 THFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDT 351

Query: 302 TLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEV 361
            +  +G  N        EILL + +     ++ IK DYVK+PI + A   I ++L  ++V
Sbjct: 352 IIFYSGVVNYNTTNFKKEILLDR-SGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDV 411

Query: 362 ETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYS 421
                  +PYGG M  IS S  PF HRA   Y+I Y   W +Q    NE+H+NWIR  Y+
Sbjct: 412 GVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQED--NEKHINWIRNVYN 471

Query: 422 FMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDP 481
           F TP+VS +PR AY+NYRDLD+G  N     +Y +A IWG KYFG NF +LV+VKT VDP
Sbjct: 472 FTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDP 531

Query: 482 SNFFRHEQSIPPLTL 489
            NFFR+EQSIPPL L
Sbjct: 532 DNFFRNEQSIPPLPL 542

BLAST of Cucsat.G8172 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-139
Identity = 248/493 (50.30%), Postives = 331/493 (67.14%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           IYT  +  Y S+L S  QN RF T   T KPLVI+ PS+VSH+Q++++CSK  GLQIR R
Sbjct: 52  IYTQHDQLYMSVLNSTIQNLRF-TSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTR 111

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EGLSYIS +PF +VDL N+ +++VD  + TAWV++GAT+GE+YY I E +   +
Sbjct: 112 SGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFS 171

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FP G CPTVG+GGHFSGGGYG LMR YGLAADN+IDA+LV+ +G V DR++MGEDLFWAI
Sbjct: 172 FPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAI 231

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIG 241
           RGGGG +FGI+  WK+KLV VP   T   + K+++     K+  +WQ +A + ++ L++ 
Sbjct: 232 RGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLT 291

Query: 242 INLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQS 301
            +    N T    +N T     F S++LG  D LV +MN + P LG+ K +CKE SWI +
Sbjct: 292 THFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDT 351

Query: 302 TLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEV 361
           T+  +G  N        EILL + +     ++ IK DYVK+ I + A   I ++L  +EV
Sbjct: 352 TIFYSGVVNYNTANFKKEILLDR-SAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEV 411

Query: 362 ETSQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYS 421
                 L+PYGG M  IS S  PF HRA  +Y++ YT  W +Q    NE+H+NW+R  Y+
Sbjct: 412 GVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEKQED--NEKHINWVRSVYN 471

Query: 422 FMTPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDP 481
           F TP+VS +PR AY+NYRDLD+G  N     +Y +A IWG KYFG NF +LV+VKT  DP
Sbjct: 472 FTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADP 531

Query: 482 SNFFRHEQSIPPL 487
           +NFFR+EQSIPPL
Sbjct: 532 NNFFRNEQSIPPL 540

BLAST of Cucsat.G8172 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 990 bits (2559), Expect = 0.0
Identity = 488/489 (99.80%), Postives = 488/489 (99.80%), Query Frame = 0

Query: 1   PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRI 60
           PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSH LQIRI
Sbjct: 47  PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRI 106

Query: 61  RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 120
           RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL
Sbjct: 107 RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 166

Query: 121 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 180
           AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA
Sbjct: 167 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 226

Query: 181 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 240
           IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG
Sbjct: 227 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 286

Query: 241 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 300
           INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI
Sbjct: 287 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 346

Query: 301 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 360
           AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL
Sbjct: 347 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 406

Query: 361 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 420
           FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS
Sbjct: 407 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 466

Query: 421 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 480
           NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE
Sbjct: 467 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 526

Query: 481 QSIPPLTLS 489
           QSIPPLTLS
Sbjct: 527 QSIPPLTLS 535

BLAST of Cucsat.G8172 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 460/489 (94.07%), Postives = 474/489 (96.93%), Query Frame = 0

Query: 1   PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRI 60
           PIYTPINSSY+S LRSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSHGLQIRI
Sbjct: 47  PIYTPINSSYTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRI 106

Query: 61  RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 120
           RSGGHDTEGLSYISDLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTL
Sbjct: 107 RSGGHDTEGLSYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTL 166

Query: 121 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 180
           AFPAGVCPTVG+GG FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWA
Sbjct: 167 AFPAGVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWA 226

Query: 181 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 240
           IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIG
Sbjct: 227 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIG 286

Query: 241 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 300
           INLTGGNPTKGVKRNPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLI
Sbjct: 287 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLI 346

Query: 301 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 360
           AAGFTNGQPLEILL+KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFL
Sbjct: 347 AAGFTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFL 406

Query: 361 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 420
           FPYGGKM+NISSSKTPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVS
Sbjct: 407 FPYGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVS 466

Query: 421 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 480
           NSPRAAYVNYRDLDIGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+
Sbjct: 467 NSPRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQ 526

Query: 481 QSIPPLTLS 489
           QSIPPLTLS
Sbjct: 527 QSIPPLTLS 535

BLAST of Cucsat.G8172 vs. NCBI nr
Match: XP_038895165.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 428/489 (87.53%), Postives = 461/489 (94.27%), Query Frame = 0

Query: 1   PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRI 60
           PIYTPINSSYSS+L SYAQN+RFSTQ+KTLKPLVIIKPSH+SH+Q+ VICSKSHGLQIRI
Sbjct: 48  PIYTPINSSYSSLLTSYAQNTRFSTQHKTLKPLVIIKPSHISHIQAIVICSKSHGLQIRI 107

Query: 61  RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 120
           RSGGHDTEGLSYISD+PF+V+DLINLKSIEVDT NNTAWVQSGATIGELYY+IAEKSRTL
Sbjct: 108 RSGGHDTEGLSYISDVPFIVIDLINLKSIEVDTKNNTAWVQSGATIGELYYKIAEKSRTL 167

Query: 121 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 180
           AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNV+DAYLVD +G VFDRE MGEDLFWA
Sbjct: 168 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVMDAYLVDDDGRVFDRETMGEDLFWA 227

Query: 181 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 240
           IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRL+KSLDKNTKKIVYQWQYVAN+ME+ LLIG
Sbjct: 228 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLEKSLDKNTKKIVYQWQYVANKMEDTLLIG 287

Query: 241 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 300
           INLTGGNPT+GVKRNPTASFFSL+LGKT+KL+TIMNTT P LGLTK+NC+ETSWIQSTLI
Sbjct: 288 INLTGGNPTQGVKRNPTASFFSLFLGKTNKLITIMNTTFPELGLTKSNCEETSWIQSTLI 347

Query: 301 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 360
           +AGFTNG+ LE+LL+KP +   ISYKIKSDYVKQPISQ A KGIW+RLKSQ  ETSQLFL
Sbjct: 348 SAGFTNGEALEVLLAKPPIKT-ISYKIKSDYVKQPISQQALKGIWERLKSQVTETSQLFL 407

Query: 361 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 420
           FPYGGKMS ISSSK PFSHRAEFLYKISYTVGW EQ   ANERHLNWIRE Y +MTPFVS
Sbjct: 408 FPYGGKMSKISSSKIPFSHRAEFLYKISYTVGWTEQSIVANERHLNWIRELYDYMTPFVS 467

Query: 421 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 480
           NSPRAAY+NYRDLDIGTNNKYG TSY++ASIWGLKYFGNNFK+LVQVKT VDPSNFFRHE
Sbjct: 468 NSPRAAYINYRDLDIGTNNKYGNTSYKQASIWGLKYFGNNFKRLVQVKTKVDPSNFFRHE 527

Query: 481 QSIPPLTLS 489
           QSIPPL+LS
Sbjct: 528 QSIPPLSLS 535

BLAST of Cucsat.G8172 vs. NCBI nr
Match: XP_023520034.1 (tetrahydrocannabinolic acid synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 757 bits (1955), Expect = 1.52e-271
Identity = 367/489 (75.05%), Postives = 418/489 (85.48%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           IYTPINSSYSS+L SYA N R ST   T  P VIIKPSH+SH+Q+ V+CSKSHG +IR R
Sbjct: 45  IYTPINSSYSSLLSSYALNPRVSTP-ATPNPRVIIKPSHLSHIQAAVVCSKSHGFRIRTR 104

Query: 62  SGGHDTEGLSYISD-LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 121
           SGGHD EGLSYIS  LPF+VVDL NL SI VD  NNTAWVQSGATIGE+YY IA+KSRTL
Sbjct: 105 SGGHDAEGLSYISHHLPFIVVDLKNLNSIAVDIENNTAWVQSGATIGEVYYEIAKKSRTL 164

Query: 122 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 181
           AFPAGVCPTVG GGH SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWA
Sbjct: 165 AFPAGVCPTVGFGGHLSGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWA 224

Query: 182 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 241
           IRGGGGGSFGIVVGWKVKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G
Sbjct: 225 IRGGGGGSFGIVVGWKVKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLG 284

Query: 242 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 301
           +NLTGGNPT+G K N  A+FFSL+LG + +L+  MNTT P LGLT  +C++TSWIQSTL+
Sbjct: 285 VNLTGGNPTEGAKPNHKATFFSLFLGNSHQLIATMNTTFPQLGLTNNDCQQTSWIQSTLL 344

Query: 302 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 361
           AAGFTN  PLE LL+K T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL L
Sbjct: 345 AAGFTNRDPLEFLLTK-TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLL 404

Query: 362 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 421
           FPYGG+MS I + +TPFSHRAEF+YKISY VGWAEQ   ANER+LNW+REFY +M+PFVS
Sbjct: 405 FPYGGRMSQIPTWETPFSHRAEFMYKISYVVGWAEQSMEANERNLNWVREFYEYMSPFVS 464

Query: 422 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 481
           NSPRAAYVNYRDLDIG NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHE
Sbjct: 465 NSPRAAYVNYRDLDIGMNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHE 524

Query: 482 QSIPPLTLS 489
           QS+PPLTLS
Sbjct: 525 QSVPPLTLS 531

BLAST of Cucsat.G8172 vs. NCBI nr
Match: XP_022927076.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 747 bits (1929), Expect = 1.37e-267
Identity = 362/489 (74.03%), Postives = 415/489 (84.87%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           IYTPINSSYSS+L SYA N R S    T  P VIIKPSH+SH+Q+ V+CSKSHG QIR R
Sbjct: 45  IYTPINSSYSSLLTSYALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTR 104

Query: 62  SGGHDTEGLSYISD-LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 121
           SGGHD EGLSYIS  LPF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTL
Sbjct: 105 SGGHDAEGLSYISHHLPFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTL 164

Query: 122 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 181
           AFPAGVCPTVG GGH SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWA
Sbjct: 165 AFPAGVCPTVGFGGHLSGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWA 224

Query: 182 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 241
           IRGGGGGSFGIVVGWKVKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G
Sbjct: 225 IRGGGGGSFGIVVGWKVKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLG 284

Query: 242 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 301
           +NLTGGNPT+G K N  A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+
Sbjct: 285 VNLTGGNPTQGAKPNHKATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLL 344

Query: 302 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 361
           AAGFT+  PLE LL+K T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL L
Sbjct: 345 AAGFTSRDPLEFLLTK-TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLL 404

Query: 362 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 421
           FPYGG+MS I + +TPFSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVS
Sbjct: 405 FPYGGRMSKIPTWETPFSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVS 464

Query: 422 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 481
           NSPRAAYVNYRDLDIG NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHE
Sbjct: 465 NSPRAAYVNYRDLDIGMNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHE 524

Query: 482 QSIPPLTLS 489
           QS+PPL LS
Sbjct: 525 QSVPPLALS 531

BLAST of Cucsat.G8172 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 460/489 (94.07%), Postives = 474/489 (96.93%), Query Frame = 0

Query: 1   PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRI 60
           PIYTPINSSY+S LRSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSHGLQIRI
Sbjct: 47  PIYTPINSSYTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRI 106

Query: 61  RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 120
           RSGGHDTEGLSYISDLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTL
Sbjct: 107 RSGGHDTEGLSYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTL 166

Query: 121 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 180
           AFPAGVCPTVG+GG FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWA
Sbjct: 167 AFPAGVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWA 226

Query: 181 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 240
           IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIG
Sbjct: 227 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIG 286

Query: 241 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 300
           INLTGGNPTKGVKRNPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLI
Sbjct: 287 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLI 346

Query: 301 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 360
           AAGFTNGQPLEILL+KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFL
Sbjct: 347 AAGFTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFL 406

Query: 361 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 420
           FPYGGKM+NISSSKTPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVS
Sbjct: 407 FPYGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVS 466

Query: 421 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 480
           NSPRAAYVNYRDLDIGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+
Sbjct: 467 NSPRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQ 526

Query: 481 QSIPPLTLS 489
           QSIPPLTLS
Sbjct: 527 QSIPPLTLS 535

BLAST of Cucsat.G8172 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 460/489 (94.07%), Postives = 474/489 (96.93%), Query Frame = 0

Query: 1   PIYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRI 60
           PIYTPINSSY+S LRSYAQN RFSTQYKTLKPL IIKPSHVSH+QSTVICSKSHGLQIRI
Sbjct: 47  PIYTPINSSYTSTLRSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRI 106

Query: 61  RSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 120
           RSGGHDTEGLSYISDLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTL
Sbjct: 107 RSGGHDTEGLSYISDLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTL 166

Query: 121 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 180
           AFPAGVCPTVG+GG FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGE LFWA
Sbjct: 167 AFPAGVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWA 226

Query: 181 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 240
           IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIG
Sbjct: 227 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIG 286

Query: 241 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 300
           INLTGGNPTKGVKRNPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLI
Sbjct: 287 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLI 346

Query: 301 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 360
           AAGFTNGQPLEILL+KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFL
Sbjct: 347 AAGFTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFL 406

Query: 361 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 420
           FPYGGKM+NISSSKTPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVS
Sbjct: 407 FPYGGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVS 466

Query: 421 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 480
           NSPRAAYVNYRDLDIGTNNK GKTSYEEASIWG+KYFGNNFK+LVQVKT VDPSNFFRH+
Sbjct: 467 NSPRAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQ 526

Query: 481 QSIPPLTLS 489
           QSIPPLTLS
Sbjct: 527 QSIPPLTLS 535

BLAST of Cucsat.G8172 vs. ExPASy TrEMBL
Match: A0A6J1EGP1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111434010 PE=3 SV=1)

HSP 1 Score: 747 bits (1929), Expect = 6.63e-268
Identity = 362/489 (74.03%), Postives = 415/489 (84.87%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           IYTPINSSYSS+L SYA N R S    T  P VIIKPSH+SH+Q+ V+CSKSHG QIR R
Sbjct: 45  IYTPINSSYSSLLTSYALNPRVSIP-ATPNPHVIIKPSHLSHIQAAVVCSKSHGFQIRTR 104

Query: 62  SGGHDTEGLSYISD-LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTL 121
           SGGHD EGLSYIS  LPF++VDL NL SI V+  NNTAWVQSGATIGE+YY IA+KSRTL
Sbjct: 105 SGGHDAEGLSYISHHLPFIIVDLKNLNSITVNIENNTAWVQSGATIGEVYYEIAKKSRTL 164

Query: 122 AFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWA 181
           AFPAGVCPTVG GGH SGGGYGWLMRKYGLAADNV+DAYLVDA G V DREAMGEDLFWA
Sbjct: 165 AFPAGVCPTVGFGGHLSGGGYGWLMRKYGLAADNVVDAYLVDAYGKVLDREAMGEDLFWA 224

Query: 182 IRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG 241
           IRGGGGGSFGIVVGWKVKLV VP IVTTC+L+K+LD   KK+VYQWQYVAN+M+E LL+G
Sbjct: 225 IRGGGGGSFGIVVGWKVKLVAVPAIVTTCQLEKNLDGYAKKLVYQWQYVANKMDENLLLG 284

Query: 242 INLTGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLI 301
           +NLTGGNPT+G K N  A+FFSL+LG +  L+  MNTT P LGLT  +C++TSWIQSTL+
Sbjct: 285 VNLTGGNPTQGAKPNHKATFFSLFLGNSHDLIATMNTTFPQLGLTNNDCQQTSWIQSTLL 344

Query: 302 AAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFL 361
           AAGFT+  PLE LL+K T  N ++YKIKSDYV +PIS+HAFKGIWDRLKS ++E++QL L
Sbjct: 345 AAGFTSRDPLEFLLTK-TSFNTVAYKIKSDYVNKPISKHAFKGIWDRLKSPKMESAQLLL 404

Query: 362 FPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVS 421
           FPYGG+MS I + +TPFSHRAEF+YKISY VGWAEQ   A+ER+LNW+REFY +M+PFVS
Sbjct: 405 FPYGGRMSKIPTWETPFSHRAEFMYKISYVVGWAEQSREASERNLNWVREFYEYMSPFVS 464

Query: 422 NSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHE 481
           NSPRAAYVNYRDLDIG NN+YG TSYEEA +WG  YFG+NFK+LV+VK  VDP NFFRHE
Sbjct: 465 NSPRAAYVNYRDLDIGMNNEYGSTSYEEARVWGEMYFGDNFKRLVRVKNKVDPLNFFRHE 524

Query: 482 QSIPPLTLS 489
           QS+PPL LS
Sbjct: 525 QSVPPLALS 531

BLAST of Cucsat.G8172 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 6.43e-239
Identity = 330/491 (67.21%), Postives = 401/491 (81.67%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           IYTPIN SYSS+L    +N RFS+  +T KPL+II PS+VSH+Q+ VICSK+HGLQIRIR
Sbjct: 51  IYTPINPSYSSVLNFSVRNLRFSSA-ETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIR 110

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EGLSY++ LPF+++DLINLKSI V+  N+TAW+QSGAT+GELYY IA+KSRTLA
Sbjct: 111 SGGHDIEGLSYVAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLA 170

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FPAGVCPTVG+GGHFSGGGYG ++RKYGLAADNVIDAYL+DANG  +DRE+MGEDLFWAI
Sbjct: 171 FPAGVCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAI 230

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGI 241
           RGGGGGSFGIVV WKVKLV VP IVTTC  D++LD++T  ++++WQ VA +++E L  GI
Sbjct: 231 RGGGGGSFGIVVAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGI 290

Query: 242 NLTGGNPTK--GVKR-NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQST 301
            L GGN +   GVK+ NPTA+FFSL+LGK+D+L+ I+NT  P L L K +C ETSWI+ST
Sbjct: 291 ILAGGNASTEGGVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIEST 350

Query: 302 LIAAGFTNGQP---LEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVET 361
           L+AA  T  Q    LE LL++  L+   + K+KSDYVK+PI +   +GIW+RLK+Q++E 
Sbjct: 351 LVAA--TRNQTIESLESLLNRTPLTIE-NTKLKSDYVKEPIPKATIEGIWERLKAQDIEV 410

Query: 362 SQLFLFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFM 421
            QL   PYGG+MS IS S+TPFSHRA  LYKI Y VGW EQ   A ERHL+WIRE Y +M
Sbjct: 411 PQLVFVPYGGRMSRISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYM 470

Query: 422 TPFVSNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSN 481
           TPFVS SPRA YVNYRDLDIGTNNKYGKTSY++AS+WG KYFGNNF +LV +K+ VDPSN
Sbjct: 471 TPFVSKSPRATYVNYRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSN 530

Query: 482 FFRHEQSIPPL 486
           FF++EQSIPPL
Sbjct: 531 FFKNEQSIPPL 537

BLAST of Cucsat.G8172 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 671 bits (1730), Expect = 1.79e-237
Identity = 326/488 (66.80%), Postives = 389/488 (79.71%), Query Frame = 0

Query: 2   IYTPINSSYSSILRSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIR 61
           +YTP+NSSYSS+L    +N RFST   T KPL II P HVSH+Q+ V+CS++HGLQIR R
Sbjct: 55  VYTPLNSSYSSVLDFSIRNLRFSTA-DTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTR 114

Query: 62  SGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLA 121
           SGGHD EGLS +S LPF+++DLINL+SI +D  NNTAWVQSGAT+GELYY IAEKSRTLA
Sbjct: 115 SGGHDFEGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLA 174

Query: 122 FPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAI 181
           FPAG CPTVGIGGHFSGGGYG ++RK+GLAADNVIDAYLVDANG   DRE MGEDLFWAI
Sbjct: 175 FPAGACPTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAI 234

Query: 182 RGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGI 241
           RGGGGGSFGIVV WKVKLV VP  VT+C  +++L++   K++++WQYVAN+++E L +GI
Sbjct: 235 RGGGGGSFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGI 294

Query: 242 NLTGGNPTK--GVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTL 301
            L GGN T   G K NPTASFFSL+LGK D+   I+NTT P LGL K NC E+SWI+S L
Sbjct: 295 LLMGGNFTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESAL 354

Query: 302 IAAGFTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLF 361
           ++AGF  G  LE LL++  L+N  S KIKSDYVK+PIS+   +GIW+RLKS++V   QL 
Sbjct: 355 LSAGFKIGDSLEALLNRTPLTNG-SIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLL 414

Query: 362 LFPYGGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFV 421
             PYGG+MS IS S+ PFSHRA  LYKI Y V W +Q     +RHL WIRE YS+MTPFV
Sbjct: 415 FVPYGGRMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFV 474

Query: 422 SNSPRAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRH 481
           S SPR+AYVNYRDLDIG+NNKYGKTS++ AS+WG KYFGNNF +LV VKT VDP +FFRH
Sbjct: 475 SKSPRSAYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRH 534

Query: 482 EQSIPPLT 487
           EQSIP ++
Sbjct: 535 EQSIPTIS 540