Cucsat.G8171 (gene) Cucumber (B10) v3

BLAST of Cucsat.G8171 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.1e-149
Identity = 265/543 (48.80%), Postives = 364/543 (67.03%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   H P+     KLV+T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF +  TPKPL+I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F+VVDL N+ S+ +DV   TAWVE+GATLGE+YY I +K+  L FP G CPTVGVG
Sbjct: 123 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K +++L L   +    ++   
Sbjct: 243 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++F G V+ L+ ++NK+FPELG+ K +CKE SWI++    S    F  
Sbjct: 303 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+P+ + A+  I ++L  +D+      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M +ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+ VDP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 542

BLAST of Cucsat.G8171 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 257/509 (50.49%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++CL  + P      KLV+T  +  + S+LN   +N RF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E+EL L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +   +   E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           YFG NFNRLVKVK+ VDP NFF +EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Cucsat.G8171 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 262/538 (48.70%), Postives = 371/538 (68.96%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   + P+     KLV+T  N  + S
Sbjct: 3   CSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN    N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F++VDL N++S+ +DV   TAWVE+GATLGE+YY + +K+  L   AG CPTV  G
Sbjct: 123 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHF GGGYG L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+V
Sbjct: 183 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK++LV VP + T+ S K+ +E  + +KL+++WQ +A K +++L L   +    ++  +
Sbjct: 243 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 302

Query: 317 GGKINPIALFF-TLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G     I  +F ++FLG V+ L+ ++NK+FPELG+ K +C++ SWI++    S    +  
Sbjct: 303 GKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
           ++   E LL+R+   +G+FK+K DYVK+P+ ++    I ++L  +DI     A+ PYGG 
Sbjct: 363 DNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGILY++ YI  WE++  E  ++HLNWIR IY++MTP+VSK+PR A
Sbjct: 423 MDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           Y+NYRDLDIG N+     NY QA ++G KYFG NF+RLVKVK+ VDP NFF +EQSIP
Sbjct: 483 YLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIP 537

BLAST of Cucsat.G8171 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.5e-148
Identity = 256/509 (50.29%), Postives = 356/509 (69.94%), Query Frame = 0

Query: 33  EEFIQCLLHHSPDTYLITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++C   + P      KLV+T  +  + S+LN   +N RF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEELFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E+EL L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +       E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI+Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSDVDPYNFFWHEQSIP 538
           YFG NFNRLVKVK+ VDP NFF +EQSIP
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIP 538

BLAST of Cucsat.G8171 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.5e-146
Identity = 257/543 (47.33%), Postives = 363/543 (66.85%), Query Frame = 0

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   + P+     K ++T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF++  TPKPL+I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGLSY+
Sbjct: 63  VLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F +VDL N+ +V VD+   TAWVE+GATLGE+YY I + +    FP G CPTVGVG
Sbjct: 123 SQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K +++L L   +    ++   
Sbjct: 243 AWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++FLG V+ L+ ++NK+FPELG+ K +CKE SWI++    S    +  
Sbjct: 303 GKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+ + + A+  I ++L  +++      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+  DP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 542

BLAST of Cucsat.G8171 vs. NCBI nr
Match: XP_011659211.2 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653319.1 hypothetical protein Csa_023346 [Cucumis sativus])

HSP 1 Score: 1095 bits (2831), Expect = 0.0
Identity = 542/544 (99.63%), Postives = 543/544 (99.82%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MK SPLIPLSL+FVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540
           VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

Query: 541 DHMV 544
           DHMV
Sbjct: 541 DHMV 544

BLAST of Cucsat.G8171 vs. NCBI nr
Match: XP_008445721.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 500/542 (92.25%), Postives = 523/542 (96.49%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M LSPL+PLSL+F+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

BLAST of Cucsat.G8171 vs. NCBI nr
Match: KAA0047096.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05062.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 477/542 (88.01%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M LSPL+PLSL+F+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

BLAST of Cucsat.G8171 vs. NCBI nr
Match: XP_038895262.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 907 bits (2343), Expect = 0.0
Identity = 442/545 (81.10%), Postives = 498/545 (91.38%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSS-INHEEFIQCLLHHSPDTYLITKLVHTPINS 60
           MK SPLI LSL F++VCSSSSL  A SSS I HEEF+QCLLHHSP+TY ITKLV+ PINS
Sbjct: 1   MKFSPLILLSLAFIIVCSSSSLLSATSSSYIKHEEFLQCLLHHSPNTYSITKLVYAPINS 60

Query: 61  SFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120
           S+SSLLNFP+RNSRFSTPN P PLLIITP+NISHIQAA++CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFPSRNSRFSTPNIPNPLLIITPTNISHIQAAIICSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYVAY  FIVVDLINL+SVTVD E+S+AWV+SGATLGELYY+I +KS+TL FPAG+CPT
Sbjct: 121 LSYVAYVPFIVVDLINLRSVTVDAEKSSAWVQSGATLGELYYRIAEKSQTLAFPAGVCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGG+F
Sbjct: 181 VGVGGHFSGGGYGILLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGNF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVS 300
           G+VVAWKVKLVPVP TVTIC+AKR+LEE AI+LIDQWQYV NKLEE+LFL+IF NGGK S
Sbjct: 241 GVVVAWKVKLVPVPETVTICAAKRTLEEGAIELIDQWQYVGNKLEEDLFLSIFLNGGKNS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGK+NP ALFF+LFLG  +EL+AIL K FPELGLTKE+CKEKSWI SA SAANRFQI
Sbjct: 301 AQGGGKVNPTALFFSLFLGKADELVAILKKKFPELGLTKEDCKEKSWIGSAVSAANRFQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           EDQPLEALLNRTP+ +GS K+KSDYVKE +TK  I+GIW+RL+SQDIEGVTLA+IPYGGR
Sbjct: 361 EDQPLEALLNRTPLANGSLKIKSDYVKELITKVGIEGIWERLKSQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M QIS+S+IPF HRAGI YQIGYI+GW+E+G++AEKRH+NWIREIYSYMTPFVSKSPRAA
Sbjct: 421 MGQISDSEIPFSHRAGIRYQIGYIVGWKEQGLDAEKRHINWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRD DIGSNNKYGK++YK+A V+G KYFGNNFNRLV++K+ VDP NFF HEQSIPLS
Sbjct: 481 YVNYRDHDIGSNNKYGKVSYKRARVWGLKYFGNNFNRLVRLKTKVDPSNFFRHEQSIPLS 540

Query: 541 SDHMV 544
            +H +
Sbjct: 541 PNHRI 545

BLAST of Cucsat.G8171 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 439/543 (80.85%), Postives = 485/543 (89.32%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSS-INHEEFIQCLLHHSPDTYLITKLVHTPINS 60
           MK SPLIPLSL F+++CSSSSL  A SSS I +EEF+QCLLHHSP+TY IT LV+ P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120
           S+SSLLNF  RN RFSTP  PKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPT 180
           LSYVAYH FIVVDLINL SV VDVEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPT
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGGHFSGGGYG+LLRKYG+AADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVS 300
           GIVVAWKVKLVPVPATVTIC+A R+ +E AIKLIDQWQYVANKL+E+LFL+I   GGK+S
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 AQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQI 360
           AQ GGKINP ALFFTLFLG  +EL+ IL   FPEL L KE+CKE SWIESA   AN  QI
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           +DQPLEA LNRTP+ +GSFK+KSDYVKEP+TKAAI+GIW+RL+SQDIEGV L +IPYGGR
Sbjct: 361 DDQPLEAFLNRTPLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M+QISES+IPFPHR G LY+IGYI+GWEE+GV+AEKRHLNWIREIYSYMTPFVSKSPRAA
Sbjct: 421 MSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSN+KYGK +YK+AC++G KYFGNNFNRLV VK++VDPY+FF HEQSIP  
Sbjct: 481 YVNYRDLDIGSNDKYGKTSYKRACIWGKKYFGNNFNRLVHVKNEVDPYDFFRHEQSIPSF 540

Query: 541 SDH 542
             H
Sbjct: 541 PHH 542

BLAST of Cucsat.G8171 vs. ExPASy TrEMBL
Match: A0A1S3BDD9 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488665 PE=3 SV=1)

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 500/542 (92.25%), Postives = 523/542 (96.49%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M LSPL+PLSL+F+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

BLAST of Cucsat.G8171 vs. ExPASy TrEMBL
Match: A0A5D3C1K3 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00270 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 477/542 (88.01%), Postives = 500/542 (92.25%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           M LSPL+PLSL+F+VVCSSSSLW A SSSI HEEF+QCLLHHSPDT  I KLVHTPINSS
Sbjct: 1   MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEE+LFLAI WNGGKVSA
Sbjct: 241 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGS-FKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HGS FKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 361 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 420

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 421 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 480

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNK                       LVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 481 YVNYRDLDIGSNNK-----------------------LVKVKTKVDPYNFFWHEQSIPLS 519

BLAST of Cucsat.G8171 vs. ExPASy TrEMBL
Match: A0A5A7TYU2 (Cannabidiolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00260 PE=3 SV=1)

HSP 1 Score: 872 bits (2252), Expect = 6.85e-316
Identity = 422/538 (78.44%), Postives = 482/538 (89.59%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MK S L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH F+VVDLINL SVT++VEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+E+LFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QISES+IPF HRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPL 538
           VNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

BLAST of Cucsat.G8171 vs. ExPASy TrEMBL
Match: A0A1S3BCV7 (cannabidiolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488658 PE=3 SV=1)

HSP 1 Score: 872 bits (2252), Expect = 6.85e-316
Identity = 422/538 (78.44%), Postives = 482/538 (89.59%), Query Frame = 0

Query: 1   MKLSPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60
           MK S L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CLLHHSPD+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH F+VVDLINL SVT++VEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+E+LFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QISES+IPF HRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPL 538
           VNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK++VDPYNFF HEQSIP+
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

BLAST of Cucsat.G8171 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 2.42e-284
Identity = 386/536 (72.01%), Postives = 456/536 (85.07%), Query Frame = 0

Query: 4   SPLIPLSLLFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPD--TYLITKLVHTPINSSF 63
           SPLI LSL F+ + SSSSLW +A  +  HE F+QCLL HS +   Y I+K++HTPINSS+
Sbjct: 5   SPLISLSLAFIFL-SSSSLWASAFPN-KHESFLQCLLDHSLEYSNYSISKVIHTPINSSY 64

Query: 64  SSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLS 123
           SS+L+F  RN RFST  TPKPLLI+TPS++SHIQAAV+CSKS+G QIR RSGGHDFEGLS
Sbjct: 65  SSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLS 124

Query: 124 YVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVG 183
           YVA+  FI+VDLINL+S+++D++ +TAWV+SGATLGELYY I +KSRTL FPAGICPTVG
Sbjct: 125 YVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVG 184

Query: 184 VGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGI 243
           VGGHFSGGGYG LLRKYG+AADNVIDAYLVDA+G+FH+RESMGEDLFWAIRGGGGGSFGI
Sbjct: 185 VGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGI 244

Query: 244 VVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSAQ 303
           VVAWKVKLVPVPATVT+CSA R+LEE A+K+  +WQYVANKL+E LFL I W GGK+++Q
Sbjct: 245 VVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQ 304

Query: 304 EGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIED 363
           EGGK NP+A FF+LFLG  +EL++ILN  FPEL L K++C E SWIES       F  E 
Sbjct: 305 EGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTE- 364

Query: 364 QPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMN 423
             LEALL+RTP+T+ S K+KSDYVKEPM++A +QGIWKRL+SQDIEG  +  +PYGGRM+
Sbjct: 365 LTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 424

Query: 424 QISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYV 483
           QISES+ PF HRAG LY+IGYI GWEE+G++AEKRHL WIRE+Y YMTPFVSKSPRAAY 
Sbjct: 425 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 484

Query: 484 NYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIP 537
           NYRDLDIGSNNK+GK +YKQA V+G KYFGNNFNRLV VK+ VDPY+FF HEQSIP
Sbjct: 485 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 537