Cucsat.G8167 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G8167
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	WAT1-related protein
Location	ctg1557: 1915606 .. 1918141 (+)
RNA-Seq Expression	Cucsat.G8167
Synteny	Cucsat.G8167

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAAGTGCCATGGAAATAGATAATAAATGGAGAAGATTTTGATGTTGTTTCTAAGGCAAATATTTGATTCCAAGATATTAGACACAGAATTTCTAGGCTGTAGTTACCCAAGCTTTCTCTATCTCCCTCTCTAACCCTCCAAAGATGCTCAAGATTACAAGAAGGGCATCATAATATTATACCAACCTTTTCGATATTGGTCGTTAATAAACTTGTGTTGTAATTCAAACGTCAGTGTCGTGACGATTCATGAGCTTATTCCATCATATACGACTGTTAAAGCTTCCAAAGAAAGGAAGCAGGATCAAAAATGAGAAAACAGCATCAATTAAGATATTCTGATACAAGTTGCATTATATTGTTTTAAATCATTTTCGTTTCCCCATTAGGCAAAAGGCTAAGCTTATATATATGTGTGTGTCTTCACAACTAAAATCATATTTCACAAGTCTTTGCCTTTGGGCAGACATCATATTGTTAGAAGAGTAAAAATGAAGCCGGTTGTGGTAATGCTTTCAATAAATCTTGCACTTGCTGGTGTGAACATACTACTTAAGGAGATTCTTAATGAAGGGATCAGTCAGCTACTCATTGTCGTTTATCGACAAACGATCTCCTCGCTTTTCTTGTTGCCAATTGCCTATTTTTGGGAAAGGTAAAGTCACCTTAACTTCTTGAAATTTTAGTTTATATTGGTTCCTTTGCCATACTAGAGCCTGCATTTTCCTTCTTACCTTGTCGAAGTTTTGATTTGTTGTATGAAAATGTTTTAAATGCTGCAAAGCTATAAAGAGTTCTCATCGTGTTTGGTGAAAATGCAGGAAAACTAGACCTCAGCTTACTGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTACTTGGGTTAGTAATTAGTATAGACATTTTTTTGTTAATTTATCGGTGTATAACTACTTTTTAAAGAATTGCATATATAGCAAAATTTATGTGTGCGATGATTTATCACTGAAATACTTTGACAGCTGAATCTAAATTTTGCTATATATGCTAATACTTTGAGCCTAAGAACTTTACGATGTTGTTTTAGTTAGTAAGAAACTTTGGAATGATGTACTAATGCAGGTTAACACTAACATTATACCTATTCCTAATTGGTCTACACTTCACCTCTGCAACTTTCTCTTGTGCATTTCTCAACCTAGTACCTGTAAACACGTTCATTCTAGCAGTATTGTTCCGGTACGTGCCGAAAGAACTGCAGTTCATTTTCTTTCCTAACATTGACAAAAATGTGATGAAAGTAACTGGTTTCAAAATATTTCTAGTATGGAAAAAGTGAACATGAAAAGCAAGGGTGGAATTGCCAAAGTGGTAGGGACACTGGTGTGCATTTCAGGAAACTTACTATTGATTCTTTACAAAGGAACACCACTAACGAGCCCTGGATCGACAAATGGGAAAACAAACGGAGGTCGTGCAACGGTTGAAGCTTATACACAAGGAAAATGGTTAATAGGTTCATTGGTTTTGACTGGAGGGTGTTTTATGTGGTCATCTTGGTTCCTTATGCAAACAAGAGTTGGGAGGGTTTATCCGTGTCAATATTCTAGTACTTGTATTATGTCATTTTTCAGTGCCCTTCAATCAGCTGTTTTGCACTTGATTATAGACAGGAAGAACTCACTGTTCATTGTGAAAGGGAAATTTGCTATCTTAAGTCTCATATATGCTGTAAGTATCAGTTTTTTCTTTTTGCCAAGTTAAGTTGCTATTTTGTTGCTAAAGTTTGTTTGTGTTTTGTGATGGGTTCAATAGGGATCAGTGGGATCAGGGTTGTGTTATGTGGGAATGTCTTGGTGTGTAAAGCAAAAAGGGCCAGTTTTCACAGCTGCTTTTACCCCTTTTATGGAAATTTTTGCTGCCATATTTGATTTCTTCATCTTACATGAACAGATTCATCTAGGAAGGTTTGCTTCTTTCTTTCCACTTTCTTTTCCTCCTATTGCTTGATTAAGACTCTCAAATTGTTTTTAACTTTGCTCTCTTGAAGTTGAAGGAAAAACAATGACGCTCCATTAAGTTCTTGACCGCTCTCTTTTTTTCTTACACTACTCTACATCTTTTTTTTCATAAAAAAATATTGATAAAAAGTAGATAACAAAATCAGATATGAAAAGGCAGAATAAGTGTTTATAAATTTAATTTTCAAAACACCGAAATGATTATCAAATGAGACCTAATAACTTCTATAAGCATTTCAAATAATATAGAGAAAGATAAATTTTGTTTTCAAGAGTAGGTTAGTTTTACTTATATTGATTATTGTTTGGACGTGATTTTAAAATTGATAAAGATCATATTTTTCATTCTTAAATACGTCTTTAATCACTCAAAATTTATTTATTTTACACTTTTAAACACAATTTTCATACTACTCATACCTCGAAACAAACTAACATCAAAATCAAAACATATACTTTGGTAGAAAGTAGCTAATGATGAAATCTTTTATTATGAAAAATTTTGGCTGAAAAATGTGAGGTTGGTGATGTGGCAGTGTGGTAGGGTCAGTGTTGATCATAAGTGGAATGTACATTCTTCTATGGGGAAAAGACAAAGAAGCAAAGGAATGTGTAGTGAAGCAAACTATATCAATAGAAGAAGCCTATCATAAATCAAACATACCATCAACAAATACAGATGCATTGGAACAAATATAAATGCATTTGCATTTCTTCTAAGGCAGCTTCTTAGTTTTATATTATTGG

Coding sequence (CDS)

ATGAAGCCGGTTGTGGTAATGCTTTCAATAAATCTTGCACTTGCTGGTGTGAACATACTACTTAAGGAGATTCTTAATGAAGGGATCAGTCAGCTACTCATTGTCGTTTATCGACAAACGATCTCCTCGCTTTTCTTGTTGCCAATTGCCTATTTTTGGGAAAGGAAAACTAGACCTCAGCTTACTGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTACTTGGGTTAACACTAACATTATACCTATTCCTAATTGGTCTACACTTCACCTCTGCAACTTTCTCTTGTGCATTTCTCAACCTAGTACCTGTAAACACGTTCATTCTAGCAGTATTGTTCCGTATGGAAAAAGTGAACATGAAAAGCAAGGGTGGAATTGCCAAAGTGGTAGGGACACTGGTGTGCATTTCAGGAAACTTACTATTGATTCTTTACAAAGGAACACCACTAACGAGCCCTGGATCGACAAATGGGAAAACAAACGGAGGTCGTGCAACGGTTGAAGCTTATACACAAGGAAAATGGTTAATAGGTTCATTGGTTTTGACTGGAGGGTGTTTTATGTGGTCATCTTGGTTCCTTATGCAAACAAGAGTTGGGAGGGTTTATCCGTGTCAATATTCTAGTACTTGTATTATGTCATTTTTCAGTGCCCTTCAATCAGCTGTTTTGCACTTGATTATAGACAGGAAGAACTCACTGTTCATTGTGAAAGGGAAATTTGCTATCTTAAGTCTCATATATGCTGGATCAGTGGGATCAGGGTTGTGTTATGTGGGAATGTCTTGGTGTGTAAAGCAAAAAGGGCCAGTTTTCACAGCTGCTTTTACCCCTTTTATGGAAATTTTTGCTGCCATATTTGATTTCTTCATCTTACATGAACAGATTCATCTAGGAAGTGTGGTAGGGTCAGTGTTGATCATAAGTGGAATGTACATTCTTCTATGGGGAAAAGACAAAGAAGCAAAGGAATGTGTAGTGAAGCAAACTATATCAATAGAAGAAGCCTATCATAAATCAAACATACCATCAACAAATACAGATGCATTGGAACAAATATAA

Protein sequence

MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI

Homology

[Close]

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot
Match: Q9LI65 (WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 9.7e-63
Identity = 116/214 (54.21%), Postives = 162/214 (75.70%), Query Frame = 0


Query: 2   EKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKW 61
           E +N+KS  G AK++GT++CI G L+L LYKGT L+   ST+ +T+    +  A TQ KW
Sbjct: 125 ETLNIKSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQ-KW 184

Query: 62  LIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLF 121
            +GS++L     +WSSWF++Q ++ RVYPCQY+ST I+SFF  +QSA+L LI +R  S++
Sbjct: 185 AMGSIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMW 244

Query: 122 IVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQ 181
           +VK KF +L+L+Y+G VGSGLCYVGMSWC++Q+G VFT++F P +++FAAIF F  LHEQ
Sbjct: 245 VVKDKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQ 304

Query: 182 IHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQ 216
           I+ GSV+GS++II G+YILLWGK K+    V KQ
Sbjct: 305 IYCGSVIGSMVIIVGLYILLWGKSKDKSASVTKQ 337

[Close]

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot
Match: Q9M130 (WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.4e-48
Identity = 100/210 (47.62%), Postives = 138/210 (65.71%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           +EK+NMKSK G+  V+G L+CI G LLL +YKG PLT       +T+       A     
Sbjct: 123 VEKLNMKSKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKL--ETHQLINNNHAMKPEN 182

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           W+IG ++L  G   + SW L+Q +V   YPCQYSST ++SFF  +Q A+L LI  R  + 
Sbjct: 183 WIIGCVLLFAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITA 242

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           +I+  K  I++++YAG+V  G+C VG SWC++++GP+FT+ FTP   IFA +FDF ILH 
Sbjct: 243 WILTDKLDIVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHR 302

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKE 211
           QI LGSVVGS ++I G+YI L GK +  KE
Sbjct: 303 QIFLGSVVGSGVVIFGLYIFLLGKVRLMKE 330

[Close]

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot
Match: Q9M129 (WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.0e-44
Identity = 93/237 (39.24%), Postives = 145/237 (61.18%), Query Frame = 0


Query: 2   EKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKW 61
           EK+++K+K G   V+GTL+ + G LLL +Y+G PLT+       +N             W
Sbjct: 124 EKLSLKTKIGYGVVLGTLISLVGGLLLTMYQGIPLTNSPEQAANSNNHTG------HENW 183

Query: 62  LIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLF 121
           + G   L  G  ++SSW L+Q ++   YPC YSST I+S F  LQ A+L LI  R    +
Sbjct: 184 IKGCFFLLTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDW 243

Query: 122 IVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQ 181
           I++ +  I++++ AG V  G+C VGMSWC+KQ+GPV +++F+P + + A +FDF ILH +
Sbjct: 244 ILRDELTIITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHRE 303

Query: 182 IHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQ--TISIEEAYHKSNIPSTNTDAL 237
           I+LGSV+GSV+++ G+YI LW + K+  EC + +  T ++EE   +    + N   L
Sbjct: 304 IYLGSVIGSVVVVIGLYIFLWSRSKQIVECKIMKLPTNTVEEEKEEEGRTNVNNGQL 354

[Close]

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot
Match: Q9M131 (WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-40
Identity = 95/230 (41.30%), Postives = 134/230 (58.26%), Query Frame = 0


Query: 5   NMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGS------TNGKTNGGRATVEAYTQ 64
           N+KSK G+ KV+GTL+CI G +LL  YKG  L++P S       N   NG   T      
Sbjct: 127 NLKSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQT------ 186

Query: 65  GKWLIGSLVLTGGCFMWSSWFLMQTRVGRVYP-CQYSSTCIMSFFSALQSAVLHLIIDRK 124
            KWL+G L L  G  + S W L Q ++   YP  +YSSTC+MS F++ Q A+L L   R 
Sbjct: 187 KKWLLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRD 246

Query: 125 NSLFIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFI 184
              +I++ KF IL  +YAG VG  +  V  SW +K  G VF + F+P   + A +FDF I
Sbjct: 247 VKDWIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLI 306

Query: 185 LHEQIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSN 228
           LH  ++LGS++GSV+ I+G+Y+ LWG+  E  + V K   S + + +K N
Sbjct: 307 LHSPLYLGSILGSVVTITGLYVFLWGRKNETDQSVSKTLNSSQFSQNKDN 350

[Close]

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot
Match: Q5XEZ0 (WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-40
Identity = 92/230 (40.00%), Postives = 134/230 (58.26%), Query Frame = 0


Query: 6   MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 65
           +K+K G+ KV+GTL+CISG L L  YKG P  S   ++          +      WL+G 
Sbjct: 135 LKTKAGMLKVIGTLICISGALFLTFYKG-PQISNSHSHSHGGASHNNNDQDKANNWLLGC 194

Query: 66  LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 125
           L LT G  + S W L Q  +   YPC+YSSTC+MS F+A Q A+L L   R  + +I+  
Sbjct: 195 LYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIIDD 254

Query: 126 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 185
           +F I  +IYAG VG  +  V  +W +K+ G VF +AF P   I A +FDF ILH  ++LG
Sbjct: 255 RFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYLG 314

Query: 186 SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDA 236
           SV+GS++ I+G+Y+ LWGK+KE +      +    EA + +  P+ + D+
Sbjct: 315 SVIGSLVTITGLYMFLWGKNKETESSTALSSGMDNEAQYTT--PNKDNDS 361

[Close]

BLAST of Cucsat.G8167 vs. NCBI nr
Match: XP_011659182.1 (WAT1-related protein At3g30340 [Cucumis sativus] >KAE8653315.1 hypothetical protein Csa_023178 [Cucumis sativus])

HSP 1 Score: 476 bits (1225), Expect = 1.52e-167
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK
Sbjct: 116 MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 175

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL
Sbjct: 176 WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 235

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE
Sbjct: 236 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 295

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI
Sbjct: 296 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 354

[Close]

BLAST of Cucsat.G8167 vs. NCBI nr
Match: XP_008444961.1 (PREDICTED: WAT1-related protein At3g30340-like [Cucumis melo])

HSP 1 Score: 463 bits (1191), Expect = 2.28e-162
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRA VEA TQGK
Sbjct: 116 MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRAMVEAETQGK 175

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLTGGCFMWSSWFLMQTRVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNSL
Sbjct: 176 WLIGSLVLTGGCFMWSSWFLMQTRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSL 235

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE
Sbjct: 236 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 295

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
            IHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNI STNTDALEQI
Sbjct: 296 PIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNISSTNTDALEQI 354

[Close]

BLAST of Cucsat.G8167 vs. NCBI nr
Match: XP_023520075.1 (WAT1-related protein At3g30340-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422 bits (1085), Expect = 1.89e-146
Identity = 206/239 (86.19%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNLLLILYKG PLTSPGS   KTNG R TV+A T GK
Sbjct: 102 MEKVDMKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKTNGARVTVQAETPGK 161

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 162 WLIGSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 221

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 222 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 281

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC VKQ +S+EE Y  SNIP+T+TDA+EQI
Sbjct: 282 QIHLGSVLGSVMIISGMYILLWGKDREAKECAVKQRVSVEEGYRNSNIPTTSTDAVEQI 340

[Close]

BLAST of Cucsat.G8167 vs. NCBI nr
Match: XP_023520074.1 (WAT1-related protein At3g30340-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422 bits (1085), Expect = 2.10e-146
Identity = 206/239 (86.19%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNLLLILYKG PLTSPGS   KTNG R TV+A T GK
Sbjct: 105 MEKVDMKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKTNGARVTVQAETPGK 164

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 165 WLIGSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 224

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 225 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 284

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC VKQ +S+EE Y  SNIP+T+TDA+EQI
Sbjct: 285 QIHLGSVLGSVMIISGMYILLWGKDREAKECAVKQRVSVEEGYRNSNIPTTSTDAVEQI 343

[Close]

BLAST of Cucsat.G8167 vs. NCBI nr
Match: XP_038895213.1 (WAT1-related protein At3g30340-like [Benincasa hispida])

HSP 1 Score: 422 bits (1085), Expect = 3.09e-146
Identity = 208/237 (87.76%), Postives = 221/237 (93.25%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MK +GG AKV+GTLVCISGNL+LILYKG PLTSPGS  GK NG  ATVEA T GK
Sbjct: 116 MEKVDMKRRGGRAKVLGTLVCISGNLILILYKGMPLTSPGSATGKLNGAGATVEAETPGK 175

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RVG V PCQYSSTCIMSFFSA+QSAVLHLIIDRKNSL
Sbjct: 176 WLIGSLVLTAGCFMWSSWFLMQSRVGEVCPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSL 235

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKGKFAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE
Sbjct: 236 FIVKGKFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 295

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALE 237
           QIHLGSVVGSVLIISGMYILLWGKD++AKEC++KQTIS+EE YH SN+PSTN DALE
Sbjct: 296 QIHLGSVVGSVLIISGMYILLWGKDRDAKECILKQTISVEEGYHNSNMPSTNADALE 352

[Close]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL
Match: A0A1S3BCC0 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 1.10e-162
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRA VEA TQGK
Sbjct: 116 MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRAMVEAETQGK 175

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLTGGCFMWSSWFLMQTRVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNSL
Sbjct: 176 WLIGSLVLTGGCFMWSSWFLMQTRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSL 235

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE
Sbjct: 236 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 295

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
            IHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNI STNTDALEQI
Sbjct: 296 PIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNISSTNTDALEQI 354

[Close]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL
Match: A0A6J1KL74 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 2.62e-146
Identity = 206/239 (86.19%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNLLLILYKG PLTSPGS   K NG R TV+A T GK
Sbjct: 102 MEKVDMKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKINGARVTVQAETPGK 161

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RV +VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 162 WLIGSLVLTAGCFMWSSWFLMQSRVCKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 221

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 222 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 281

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC VKQ +S+EE YH SNIPST TDA+EQI
Sbjct: 282 QIHLGSVIGSVMIISGMYILLWGKDREAKECAVKQRVSVEEGYHNSNIPSTTTDAVEQI 340

[Close]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL
Match: A0A6J1KMQ9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 2.91e-146
Identity = 206/239 (86.19%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNLLLILYKG PLTSPGS   K NG R TV+A T GK
Sbjct: 105 MEKVDMKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKINGARVTVQAETPGK 164

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RV +VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 165 WLIGSLVLTAGCFMWSSWFLMQSRVCKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 224

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 225 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 284

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC VKQ +S+EE YH SNIPST TDA+EQI
Sbjct: 285 QIHLGSVIGSVMIISGMYILLWGKDREAKECAVKQRVSVEEGYHNSNIPSTTTDAVEQI 343

[Close]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL
Match: A0A6J1KGI9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 4.28e-146
Identity = 206/239 (86.19%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNLLLILYKG PLTSPGS   K NG R TV+A T GK
Sbjct: 116 MEKVDMKSKGGRAKVLGTLVCISGNLLLILYKGMPLTSPGSATAKINGARVTVQAETPGK 175

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RV +VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 176 WLIGSLVLTAGCFMWSSWFLMQSRVCKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 235

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 236 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 295

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC VKQ +S+EE YH SNIPST TDA+EQI
Sbjct: 296 QIHLGSVIGSVMIISGMYILLWGKDREAKECAVKQRVSVEEGYHNSNIPSTTTDAVEQI 354

[Close]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL
Match: A0A6J1EGF7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 5.03e-144
Identity = 202/239 (84.52%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MEKVNMKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGK 60
           MEKV+MKSKGG AKV+GTLVCISGNL+LILYKG  LTSPGS   KTNG R TV+A T GK
Sbjct: 102 MEKVDMKSKGGRAKVLGTLVCISGNLILILYKGMSLTSPGSATAKTNGARVTVQADTPGK 161

Query: 61  WLIGSLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSL 120
           WLIGSLVLT GCFMWSSWFLMQ+RVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNS+
Sbjct: 162 WLIGSLVLTAGCFMWSSWFLMQSRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSV 221

Query: 121 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 180
           FIVKG FAILSL+YAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFME+FAAIFDFFILHE
Sbjct: 222 FIVKGTFAILSLVYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEVFAAIFDFFILHE 281

Query: 181 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 239
           QIHLGSV+GSV+IISGMYILLWGKD+EAKEC V Q +S+EE Y  SNIP+T+TDA+E+I
Sbjct: 282 QIHLGSVIGSVMIISGMYILLWGKDREAKECAVNQRVSVEEGYRNSNIPTTSTDAVEKI 340

The following BLAST results are available for this feature:

BLAST of Cucsat.G8167 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LI65	9.7e-63	54.21	WAT1-related protein At3g30340 OS=Arabidopsis thaliana OX=3702 GN=At3g30340 PE=2...	[more]
Q9M130	1.4e-48	47.62	WAT1-related protein At4g01440 OS=Arabidopsis thaliana OX=3702 GN=At4g01440 PE=2...	[more]
Q9M129	2.0e-44	39.24	WAT1-related protein At4g01450 OS=Arabidopsis thaliana OX=3702 GN=At4g01450 PE=2...	[more]
Q9M131	1.4e-40	41.30	WAT1-related protein At4g01430 OS=Arabidopsis thaliana OX=3702 GN=At4g01430 PE=2...	[more]
Q5XEZ0	2.3e-40	40.00	WAT1-related protein At1g01070 OS=Arabidopsis thaliana OX=3702 GN=At1g01070 PE=2...	[more]

BLAST of Cucsat.G8167 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659182.1	1.52e-167	100.00	WAT1-related protein At3g30340 [Cucumis sativus] >KAE8653315.1 hypothetical prot...	[more]
XP_008444961.1	2.28e-162	97.49	PREDICTED: WAT1-related protein At3g30340-like [Cucumis melo]	[more]
XP_023520075.1	1.89e-146	86.19	WAT1-related protein At3g30340-like isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023520074.1	2.10e-146	86.19	WAT1-related protein At3g30340-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_038895213.1	3.09e-146	87.76	WAT1-related protein At3g30340-like [Benincasa hispida]	[more]

BLAST of Cucsat.G8167 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BCC0	1.10e-162	97.49	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103488142 PE=3 SV=1	[more]
A0A6J1KL74	2.62e-146	86.19	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1	[more]
A0A6J1KMQ9	2.91e-146	86.19	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1	[more]
A0A6J1KGI9	4.28e-146	86.19	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111495572 PE=3 SV=1	[more]
A0A6J1EGF7	5.03e-144	84.52	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111434105 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 63..201 e-value: 2.9E-13 score: 50.1
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 1..221
None	No IPR available	PANTHER	PTHR31218:SF301	WAT1-RELATED PROTEIN	coord: 1..221
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 126..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G8167.T1	Cucsat.G8167.T1	mRNA
Cucsat.G8167.T5	Cucsat.G8167.T5	mRNA
Cucsat.G8167.T6	Cucsat.G8167.T6	mRNA
Cucsat.G8167.T3	Cucsat.G8167.T3	mRNA
Cucsat.G8167.T2	Cucsat.G8167.T2	mRNA
Cucsat.G8167.T4	Cucsat.G8167.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity