Cucsat.G8145 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G8145
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	endochitinase EP3-like
Location	ctg1557: 1192129 .. 1193519 (+)
RNA-Seq Expression	Cucsat.G8145
Synteny	Cucsat.G8145

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTCAAAGTCCTTAAAAATTAAGTTTACATAAGAAAAAAAACCTTCCCACACACACCAAATTTAATTTTCCATCCTTATAAATACCCTCATCTCTCCCCTTGATCTCCTAAAACAACAAACTAATCAAACAGAAAATGGAAGGACGTGGTGTAGGTGTAGCCATGCAAAAAACACTGCTCCTAACGGCCCTAGCTCTGATCGGAATCATCGCCGGAGCTGCAGCACAGAACTGTGGATGCGCGGCGGGGCTGTGCTGCAGTAGGTTCGGATTCTGCGGGAACGGCGAGGATTTCTGTGGGACCGGATGCAGAGAAGGGCCTTGCAACATTCCCCCACTTACTCCAAGTGTGAACGATGTGAATGTGGCAGAATTTGTGACGGAGGAGTTCTTCAACGGCATAATTAACGAGGCGGACGCGGGTTGCGCCGGACGGGGATTCTACAGCAGAGCTACTTTCTTAGAAGCTCTCCAGTCGTTTGATAGATTTGGAAGAATTGGATCGGTTGAGGATTCTAAAAGAGAAATCGCTGCCTTCTTTGCTCATGTTACTCACGAGACTGGACGTAAGTTTTTCCAAATTCTTTTCTCTTCTTTTTTGGTTGTTGTTGTTGTTTTTCGTATATGTTCATAACTATTAACTTCTTGAATTTTAAAAAAATTGGTTAGTTTTTTTAATATTAAGTTTATCAGAACTACTTCTCTTTTGTTTTTGTTTTATTGTTAAAAAAATTAAAAGAAAATGGAATCAAAATTTTTAATAAAATCCTACCCATTCAAATTCAAAATAAATCGAAAATTTTAATTTATAAAAATGTCGAATTGGTTAAAGACAAAGTAATTAGATACCGGTATATTGCATAAAGTTGGTAACTTGATTTATATTGTACAACAGATTTATGCTTCATAGAAGAAATAGATGGAGCAACAAGAGACTATTGCGATGAGGAAAACACTCAGTATCCATGCAACCCAAGCAAAGGGTATTTCGGTAGAGGACCAATTCAACTATCTTGGAACTTCAACTATGGCCCTGCAGGGGAGAACATCGGATTTGATGGCCTCAACAACCCGGAAATTGTTGCCACCAACCCGGTGGTGTCATTCAAGACGGCCTTGTGGTATTGGATGAACTTCGTGAGGCCGGTCATCAACCAGGGCTTCGGCGCCACCATTCGTGCCATCAATGGTGCCCTCGAATGCGACGGCGCTAACACCGCCACTGTTCAACGACGAATTCAGTTTTATAATCAATATTGTAATCAACTCAACGTTGCCCCTGGAAATAATTTGACTTGTTAGATTTTGATTTTAGTTTACTTTCCTTTTCTTGTTCTATTTTAAGCTTTCTACCAATAAATTTTTCTATCATCCATTCCTATAATTCATATA

Coding sequence (CDS)

ATGGAAGGACGTGGTGTAGGTGTAGCCATGCAAAAAACACTGCTCCTAACGGCCCTAGCTCTGATCGGAATCATCGCCGGAGCTGCAGCACAGAACTGTGGATGCGCGGCGGGGCTGTGCTGCAGTAGGTTCGGATTCTGCGGGAACGGCGAGGATTTCTGTGGGACCGGATGCAGAGAAGGGCCTTGCAACATTCCCCCACTTACTCCAAGTGTGAACGATGTGAATGTGGCAGAATTTGTGACGGAGGAGTTCTTCAACGGCATAATTAACGAGGCGGACGCGGGTTGCGCCGGACGGGGATTCTACAGCAGAGCTACTTTCTTAGAAGCTCTCCAGTCGTTTGATAGATTTGGAAGAATTGGATCGGTTGAGGATTCTAAAAGAGAAATCGCTGCCTTCTTTGCTCATGTTACTCACGAGACTGGACATTTATGCTTCATAGAAGAAATAGATGGAGCAACAAGAGACTATTGCGATGAGGAAAACACTCAGTATCCATGCAACCCAAGCAAAGGGTATTTCGGTAGAGGACCAATTCAACTATCTTGGAACTTCAACTATGGCCCTGCAGGGGAGAACATCGGATTTGATGGCCTCAACAACCCGGAAATTGTTGCCACCAACCCGGTGGTGTCATTCAAGACGGCCTTGTGGTATTGGATGAACTTCGTGAGGCCGGTCATCAACCAGGGCTTCGGCGCCACCATTCGTGCCATCAATGGTGCCCTCGAATGCGACGGCGCTAACACCGCCACTGTTCAACGACGAATTCAGTTTTATAATCAATATTGTAATCAACTCAACGTTGCCCCTGGAAATAATTTGACTTGTTAG

Protein sequence

MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC

Homology

[Close]

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot
Match: Q9M2U5 (Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.1e-113
Identity = 188/266 (70.68%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 13  TLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLTPSV 72
           +L+   L L        AQNCGC++ LCCS+FGFCGN  D+CG GC++GPC  P   P  
Sbjct: 11  SLVTILLVLQAFSNTTKAQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAP---PPA 70

Query: 73  NDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKREIA 132
           N V+VAE VT+EFFNGII++A + CAG  FYSR  FLEAL S+ RFGR+GS +DS+REIA
Sbjct: 71  NGVSVAEIVTQEFFNGIISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIA 130

Query: 133 AFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYGPAG 192
           AFFAHVTHETGH C+IEEIDGA++DYCDE  TQYPCNP+KGY+GRGPIQLSWNFNYGPAG
Sbjct: 131 AFFAHVTHETGHFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAG 190

Query: 193 ENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTA 252
             IGFDGLN PE VAT+PV+SFKTALWYW N V+PVI+QGFGATIRAINGALECDGANTA
Sbjct: 191 TAIGFDGLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTA 250

Query: 253 TVQRRIQFYNQYCNQLNVAPGNNLTC 279
           TVQ R+++Y  YC QL V PGNNLTC
Sbjct: 251 TVQARVRYYTDYCRQLGVDPGNNLTC 273

[Close]

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot
Match: P27054 (Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 5.4e-105
Identity = 179/266 (67.29%), Postives = 212/266 (79.70%), Query Frame = 0


Query: 14  LLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLTPSVN 73
           L+L A+AL+      +AQNCGCA GLCCS++G+CG GED+CGTGC++GPC      PS N
Sbjct: 7   LVLVAVALVMGPKNVSAQNCGCAEGLCCSQYGYCGTGEDYCGTGCQQGPCTTASPPPS-N 66

Query: 74  DVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKREIAA 133
           +VN A+ +T +F NGII++AD+GCAG+ FY+R  FL AL S+  FGR+GS +DSKREIAA
Sbjct: 67  NVN-ADILTADFLNGIIDQADSGCAGKNFYTRDAFLSALNSYTDFGRVGSEDDSKREIAA 126

Query: 134 FFAHVTHETGHLCFIEEIDGATRDYCDEEN-TQYPCNPSKGYFGRGPIQLSWNFNYGPAG 193
            FAH THETGH C+IEEIDGA++DYCDEE+  QYPC+ SKGY GRGPIQLSWNFNYGPAG
Sbjct: 127 AFAHFTHETGHFCYIEEIDGASKDYCDEESIAQYPCSSSKGYHGRGPIQLSWNFNYGPAG 186

Query: 194 ENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGANTA 253
               FDGL  PE V+ + VVSFKTALWYWM  VRPVINQGFGATIRAINGALECDGAN  
Sbjct: 187 SANNFDGLGAPETVSNDVVVSFKTALWYWMQHVRPVINQGFGATIRAINGALECDGANPT 246

Query: 254 TVQRRIQFYNQYCNQLNVAPGNNLTC 279
           TVQ R+ +Y +YC QL VA G+NLTC
Sbjct: 247 TVQARVNYYTEYCRQLGVATGDNLTC 270

[Close]

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot
Match: Q6K8R2 (Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 1.7e-95
Identity = 160/275 (58.18%), Postives = 205/275 (74.55%), Query Frame = 0


Query: 8   VAMQKTLLLTALALIGIIAG--AAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNI 67
           +A + +LL   LA++  ++   AAAQ+CGCA+  CCS++GFCG G D+CGTGC+ GPC++
Sbjct: 1   MARRLSLLAVVLAMVAAVSASTAAAQSCGCASDQCCSKWGFCGTGSDYCGTGCQAGPCDV 60

Query: 68  PPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVE 127
               P+ NDV+VA  VT EFF  ++ +AD GCA +GFY+R  FL A   +  FGR GSV+
Sbjct: 61  ----PATNDVSVASIVTPEFFAALVAQADDGCAAKGFYTRDAFLTAAGGYPSFGRTGSVD 120

Query: 128 DSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWN 187
           DSKREIAAFFAH  HET   C+IEEIDG +++YCDE +TQ+PC   KGY+GRGP+Q+SWN
Sbjct: 121 DSKREIAAFFAHANHETIKFCYIEEIDGPSKNYCDETSTQWPCMAGKGYYGRGPLQISWN 180

Query: 188 FNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRP--VINQGFGATIRAINGA 247
           FNYGPAG++IGFDGL +P+ VA +PV++F+TALWYW N V    V  QGFGATIRAINGA
Sbjct: 181 FNYGPAGQSIGFDGLGDPDAVARSPVLAFQTALWYWTNNVHDAFVSGQGFGATIRAINGA 240

Query: 248 LECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 279
           LECDG N   V  R+ +Y Q+C Q  V PG+NLTC
Sbjct: 241 LECDGKNPTAVSNRVAYYQQFCQQFGVDPGSNLTC 271

[Close]

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot
Match: Q7Y1Z0 (Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 9.8e-91
Identity = 154/276 (55.80%), Postives = 192/276 (69.57%), Query Frame = 0


Query: 14  LLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPC---------- 73
           L L    L+     A+AQNCGC + +CCS++G+CG G+D+CG GCR GPC          
Sbjct: 13  LALGLALLLSATGQASAQNCGCQSNMCCSKWGYCGTGKDYCGDGCRSGPCYGGGGGGGGG 72

Query: 74  -NIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIG 133
                     + V+V   VTE FFNGI N+A  GCAG+ FY+R +FL A  S+  F R  
Sbjct: 73  GGGGGGGGGGSGVSVESVVTEAFFNGIKNQAPNGCAGKNFYTRQSFLNAAHSYSGFARDR 132

Query: 134 SVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQL 193
           + +DSKREIAAFFAHVTHETGH+C+I EI+GA+ DYCD+ N Q+PC P K Y+GRGP+Q+
Sbjct: 133 TNDDSKREIAAFFAHVTHETGHMCYINEINGASMDYCDKNNKQWPCQPGKKYYGRGPLQI 192

Query: 194 SWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAING 253
           SWN+NYGPAG+NIGFDGL +P+ VA +P +SFKTALW+WMN V  V+ QGFGATIRAING
Sbjct: 193 SWNYNYGPAGQNIGFDGLRDPDRVAQDPTISFKTALWFWMNNVHQVMLQGFGATIRAING 252

Query: 254 ALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 279
           ALEC+G N   V  R+ +Y  YC Q  V PG NL C
Sbjct: 253 ALECNGKNPGAVNARVNYYKDYCRQFGVDPGGNLYC 288

[Close]

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot
Match: O04138 (Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2)

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-90
Identity = 155/281 (55.16%), Postives = 193/281 (68.68%), Query Frame = 0


Query: 8   VAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPC---- 67
           +A    L+     L+   A AAAQNCGC  G CCS++G+CG  E +CG GC+ GPC    
Sbjct: 5   MATMVALVFGLALLLSAAAPAAAQNCGCQDGYCCSQWGYCGTTEAYCGQGCQSGPCWGSG 64

Query: 68  ------NIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDR 127
                        +   V+V   VTE FFNGI N+A  GCAG+ FY+R +FL A +S+  
Sbjct: 65  GEAAAGMAGRKAGAGAGVSVESVVTEAFFNGIKNQAPNGCAGKSFYTRQSFLNAARSYSG 124

Query: 128 FGRIGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGR 187
           F    + +DSKREIAAFFAHVTHETGH+C+I EI+GA  DYCD+ N Q+PC P K Y+GR
Sbjct: 125 FANDRTNDDSKREIAAFFAHVTHETGHMCYINEINGANMDYCDKSNKQWPCQPGKKYYGR 184

Query: 188 GPIQLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATI 247
           GP+Q+SWNFNYGPAG+NIGFDGL +P+ VA +P +SFKTALW+WMN V  V++QGFGATI
Sbjct: 185 GPLQISWNFNYGPAGKNIGFDGLRDPDKVAQDPTISFKTALWFWMNNVHQVMSQGFGATI 244

Query: 248 RAINGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 279
           RAINGALEC+G N   V  R+ +Y  YC Q  V+PG NL C
Sbjct: 245 RAINGALECNGKNPGAVNARVNYYKDYCRQFGVSPGGNLYC 285

[Close]

BLAST of Cucsat.G8145 vs. NCBI nr
Match: XP_004145227.1 (endochitinase EP3 [Cucumis sativus] >KGN65869.1 hypothetical protein Csa_023230 [Cucumis sativus])

HSP 1 Score: 583 bits (1504), Expect = 1.40e-210
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60
           MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE
Sbjct: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60

Query: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120
           GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR
Sbjct: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120

Query: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180
           IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI
Sbjct: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180

Query: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240
           QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI
Sbjct: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240

Query: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC
Sbjct: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278

[Close]

BLAST of Cucsat.G8145 vs. NCBI nr
Match: XP_008444611.1 (PREDICTED: endochitinase EP3-like [Cucumis melo] >KAA0054010.1 endochitinase EP3-like [Cucumis melo var. makuwa] >TYK20696.1 endochitinase EP3-like [Cucumis melo var. makuwa])

HSP 1 Score: 559 bits (1440), Expect = 7.21e-201
Identity = 266/278 (95.68%), Postives = 271/278 (97.48%), Query Frame = 0


Query: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60
           MEG   GVAMQKTLLLTA+ALIGIIAGAAAQNCGCAAGLCCSRFGFCG GEDFCGTGCRE
Sbjct: 1   MEG---GVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCRE 60

Query: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120
           GPCNIPPLTPSVNDVNVAEFVTEEFFNGIIN+ADA CAGRGFYSRATFLEALQSFDRFGR
Sbjct: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGR 120

Query: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180
           IG+V+DSKREIAAFFAHVTHETGH CFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI
Sbjct: 121 IGTVDDSKREIAAFFAHVTHETGHFCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180

Query: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240
           QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI
Sbjct: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240

Query: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           NGALECDGANTATVQRR+QFYNQYCNQLNV PGNNLTC
Sbjct: 241 NGALECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

[Close]

BLAST of Cucsat.G8145 vs. NCBI nr
Match: XP_038894874.1 (endochitinase EP3-like [Benincasa hispida])

HSP 1 Score: 547 bits (1409), Expect = 3.84e-196
Identity = 257/274 (93.80%), Postives = 267/274 (97.45%), Query Frame = 0


Query: 5   GVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCN 64
           G GVAMQKTLL+TA+ALIGII GAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCN
Sbjct: 2   GGGVAMQKTLLITAVALIGIITGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCN 61

Query: 65  IPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSV 124
           IPPLTPSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEAL+SFDRFGRIGSV
Sbjct: 62  IPPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSV 121

Query: 125 EDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSW 184
           +DSKREIAAFF HVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNPSKGYFGRGPIQLSW
Sbjct: 122 DDSKREIAAFFGHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSW 181

Query: 185 NFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGAL 244
           NFNYGPAGENIGFDGLNNPEIVAT+PVVSFKTALWYWMNFVRPVINQGFGATIRAINGAL
Sbjct: 182 NFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFGATIRAINGAL 241

Query: 245 ECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           ECDGAN ATVQRR+QFY QYC+QL VAPGNNLTC
Sbjct: 242 ECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 275

[Close]

BLAST of Cucsat.G8145 vs. NCBI nr
Match: XP_023520063.1 (endochitinase EP3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528 bits (1360), Expect = 8.75e-189
Identity = 246/269 (91.45%), Postives = 261/269 (97.03%), Query Frame = 0


Query: 10  MQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLT 69
           MQ+ LLLT + L+GIIAGAAAQNCGCAAGLCCSRFGFCG G+DFCGTGCREGPCNIPPLT
Sbjct: 1   MQRALLLT-VTLVGIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGTGCREGPCNIPPLT 60

Query: 70  PSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKR 129
           PSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEALQSFDRFGRIGSV+DSKR
Sbjct: 61  PSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKR 120

Query: 130 EIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG 189
           EIAAFF HVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNP+KGYFGRGPIQLSWNFNYG
Sbjct: 121 EIAAFFGHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYG 180

Query: 190 PAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 249
           PAGE+IGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA
Sbjct: 181 PAGESIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 240

Query: 250 NTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           N+ATVQRR+++Y QYCNQL VAPG+NLTC
Sbjct: 241 NSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of Cucsat.G8145 vs. NCBI nr
Match: KAG7019501.1 (Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527 bits (1358), Expect = 1.77e-188
Identity = 246/269 (91.45%), Postives = 260/269 (96.65%), Query Frame = 0


Query: 10  MQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLT 69
           MQ+ LLLT + L+GIIAGAAAQNCGCAAGLCCSRFGFCG G DFCGTGCREGPCNIPPLT
Sbjct: 1   MQRALLLT-VTLVGIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLT 60

Query: 70  PSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKR 129
           PSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEALQSFDRFGRIGSV+DSKR
Sbjct: 61  PSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKR 120

Query: 130 EIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG 189
           EIAAFF HVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNP+KGYFGRGPIQLSWNFNYG
Sbjct: 121 EIAAFFGHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPNKGYFGRGPIQLSWNFNYG 180

Query: 190 PAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 249
           PAGE+IGFDGLNNPEIVATNP+VSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA
Sbjct: 181 PAGESIGFDGLNNPEIVATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 240

Query: 250 NTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           N+ATVQRR+Q+Y QYCNQL VAPG+NLTC
Sbjct: 241 NSATVQRRVQYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL
Match: A0A0A0LXT8 (Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G534750 PE=4 SV=1)

HSP 1 Score: 583 bits (1504), Expect = 6.80e-211
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60
           MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE
Sbjct: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60

Query: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120
           GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR
Sbjct: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120

Query: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180
           IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI
Sbjct: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180

Query: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240
           QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI
Sbjct: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240

Query: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC
Sbjct: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278

[Close]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL
Match: A0A5A7UFI0 (Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00900 PE=4 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 3.49e-201
Identity = 266/278 (95.68%), Postives = 271/278 (97.48%), Query Frame = 0


Query: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60
           MEG   GVAMQKTLLLTA+ALIGIIAGAAAQNCGCAAGLCCSRFGFCG GEDFCGTGCRE
Sbjct: 1   MEG---GVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCRE 60

Query: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120
           GPCNIPPLTPSVNDVNVAEFVTEEFFNGIIN+ADA CAGRGFYSRATFLEALQSFDRFGR
Sbjct: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGR 120

Query: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180
           IG+V+DSKREIAAFFAHVTHETGH CFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI
Sbjct: 121 IGTVDDSKREIAAFFAHVTHETGHFCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180

Query: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240
           QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI
Sbjct: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240

Query: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           NGALECDGANTATVQRR+QFYNQYCNQLNV PGNNLTC
Sbjct: 241 NGALECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

[Close]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL
Match: A0A1S3BAS2 (endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 3.49e-201
Identity = 266/278 (95.68%), Postives = 271/278 (97.48%), Query Frame = 0


Query: 1   MEGRGVGVAMQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCRE 60
           MEG   GVAMQKTLLLTA+ALIGIIAGAAAQNCGCAAGLCCSRFGFCG GEDFCGTGCRE
Sbjct: 1   MEG---GVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCRE 60

Query: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGR 120
           GPCNIPPLTPSVNDVNVAEFVTEEFFNGIIN+ADA CAGRGFYSRATFLEALQSFDRFGR
Sbjct: 61  GPCNIPPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGR 120

Query: 121 IGSVEDSKREIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180
           IG+V+DSKREIAAFFAHVTHETGH CFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI
Sbjct: 121 IGTVDDSKREIAAFFAHVTHETGHFCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPI 180

Query: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240
           QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI
Sbjct: 181 QLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAI 240

Query: 241 NGALECDGANTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           NGALECDGANTATVQRR+QFYNQYCNQLNV PGNNLTC
Sbjct: 241 NGALECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

[Close]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL
Match: A0A6J1KIN5 (endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 2.45e-188
Identity = 245/269 (91.08%), Postives = 259/269 (96.28%), Query Frame = 0


Query: 10  MQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLT 69
           MQ+ LLLT + L+GIIAGAAAQNCGCAAGLCCSRFGFCG G DFCGTGCREGPCNIPPLT
Sbjct: 1   MQRALLLT-VTLVGIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLT 60

Query: 70  PSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKR 129
           PSVNDV VA+FVTEEFFNGI+N+ADAGCAGRGFYSRATFLEALQSFDRFGRIGSV+DSKR
Sbjct: 61  PSVNDVVVADFVTEEFFNGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKR 120

Query: 130 EIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG 189
           EIAAFF HVTHETGHLCFIEEIDGAT+DYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG
Sbjct: 121 EIAAFFGHVTHETGHLCFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG 180

Query: 190 PAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 249
           PAGE+IGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDG 
Sbjct: 181 PAGESIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGG 240

Query: 250 NTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           N+ATVQRR+++Y QYCNQL VAPG+NLTC
Sbjct: 241 NSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL
Match: A0A6J1EGZ6 (endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 2.45e-188
Identity = 245/269 (91.08%), Postives = 260/269 (96.65%), Query Frame = 0


Query: 10  MQKTLLLTALALIGIIAGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNIPPLT 69
           MQ+ LLLT + L+GIIAGAAAQNCGCAAGLCCSRFGFCG G DFCGTGCREGPCNIPPLT
Sbjct: 1   MQRALLLT-VTLVGIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLT 60

Query: 70  PSVNDVNVAEFVTEEFFNGIINEADAGCAGRGFYSRATFLEALQSFDRFGRIGSVEDSKR 129
           PSVNDV VA+FVTEEFFNGIIN+ADAGCAGRGFYSRATFLEALQSFDRFGRIGSV+DSKR
Sbjct: 61  PSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKR 120

Query: 130 EIAAFFAHVTHETGHLCFIEEIDGATRDYCDEENTQYPCNPSKGYFGRGPIQLSWNFNYG 189
           EIAAFF HVTHETGHLCFIEEIDGAT+DYCDE+NTQYPCNPSKGYFGRGPIQLSWNFNYG
Sbjct: 121 EIAAFFGHVTHETGHLCFIEEIDGATKDYCDEDNTQYPCNPSKGYFGRGPIQLSWNFNYG 180

Query: 190 PAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 249
           PAGE+IGFDGLNNPEIVATNP+VSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA
Sbjct: 181 PAGESIGFDGLNNPEIVATNPLVSFKTALWYWMNFVRPVINQGFGATIRAINGALECDGA 240

Query: 250 NTATVQRRIQFYNQYCNQLNVAPGNNLTC 278
           N+ATVQRR+++Y QYCNQL VAPG+NLTC
Sbjct: 241 NSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

The following BLAST results are available for this feature:

BLAST of Cucsat.G8145 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U5	1.1e-113	70.68	Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1	[more]
P27054	5.4e-105	67.29	Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1	[more]
Q6K8R2	1.7e-95	58.18	Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1	[more]
Q7Y1Z0	9.8e-91	55.80	Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1	[more]
O04138	1.7e-90	55.16	Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2	[more]

BLAST of Cucsat.G8145 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145227.1	1.40e-210	100.00	endochitinase EP3 [Cucumis sativus] >KGN65869.1 hypothetical protein Csa_023230 ...	[more]
XP_008444611.1	7.21e-201	95.68	PREDICTED: endochitinase EP3-like [Cucumis melo] >KAA0054010.1 endochitinase EP3...	[more]
XP_038894874.1	3.84e-196	93.80	endochitinase EP3-like [Benincasa hispida]	[more]
XP_023520063.1	8.75e-189	91.45	endochitinase EP3 [Cucurbita pepo subsp. pepo]	[more]
KAG7019501.1	1.77e-188	91.45	Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cucsat.G8145 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXT8	6.80e-211	100.00	Chitin-binding type-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Cs...	[more]
A0A5A7UFI0	3.49e-201	95.68	Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4...	[more]
A0A1S3BAS2	3.49e-201	95.68	endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1	[more]
A0A6J1KIN5	2.45e-188	91.08	endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1	[more]
A0A6J1EGZ6	2.45e-188	91.08	endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 32..63 e-value: 6.9E-9 score: 45.5
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 27..63 e-value: 8.6E-7 score: 29.3
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 30..65 score: 9.284719
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 231..278 e-value: 3.5E-10 score: 40.1 coord: 81..223 e-value: 2.5E-40 score: 138.7
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 97..119
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 212..222
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 26..72 e-value: 3.2E-10 score: 41.9
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 26..68
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 7..278 e-value: 2.7E-101 score: 336.4
None	No IPR available	GENE3D	1.10.530.10		coord: 76..278 e-value: 4.7E-56 score: 190.9
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 149..206 e-value: 3.9E-26 score: 92.7
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 14..278
None	No IPR available	PANTHER	PTHR22595:SF131	ENDOCHITINASE EP3	coord: 14..278
None	No IPR available	CDD	cd00035	ChtBD1	coord: 27..58 e-value: 1.42597E-6 score: 42.3726
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 81..278 e-value: 1.02523E-86 score: 256.203
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 35..54
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 77..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G8145.T1	Cucsat.G8145.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0006952	defense response
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding