Cucsat.G7727 (gene) Cucumber (B10) v3

Overview
NameCucsat.G7727
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionmitogen-activated protein kinase kinase kinase 1-like
Locationctg1546: 150931 .. 157020 (-)
RNA-Seq ExpressionCucsat.G7727
SyntenyCucsat.G7727
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGGAGTGGGGAAGTGGGGTTTGAAATTGGGAGGAAGATAATGATAAATAAGAGAGGACGGTTAAAAAGAGAAAAGGAAAGTAGTGTTATTTCCTATTGATCCAACTTCACTTCACCGATTTAGAAATGGAAATTAAAAGAAAGAAAGAAAGAAAGAAAGAACGTAGGAATGATCGAAGCGGGTGAGTGAGTGAATACGTCATGGAGAGGAGGAAGAAGAAATATACTGGACTTGGTATCTGACAAGCAAACAACAAAAAGGGAAGGTCCCTCCATTTCCCCCAAATTCCCAATCCAATTCGCCAGCCAATCGCCGTCGCCCTCGGCTCGGCTGTTCTTTCGGCTACTTTTACTTCCACGTAGCACCGCGTTTTTCTCTCTCTCCGGCGTTTACCAGTCCAATTCACTCCCATCTTTCAACCCTTTTCTTCTCTCTCTCTCTCCCCCTCTCCCTCTCTCTTCCCAGCTTCCTTTCCTCAAACTTGGAGGCTGATTCATTCGCTAAACATCTCTTTTTCCCTTTTTCAATTTGGTAATTTTATTGTGGGATTCTCTTGATTTCATCGGGAAATTCTTCTTGCCGTTGCCGGTGGGATGTTTTAATGGGCTTGAGTCTCGATGAATCGTCTCCCTCAGTTTTTCTCTCCTAGGAACAAAAGAAAACCCATGGATCCCAGGAAAATTCCAGGGAAACCCAAGCTTGGCCGACTTAATGCGGCCAAGAATATTGATTATGATGCTCCTTCCTCGTCTTCTTCGCTCGAAGATTCTTCTGGGTCTCTTTACACTCGTTCCATGGAAAACCCCGATCCCTCCAGTTTTCGGATTAAAGGGCTTGACGGTGAAGTTGATCTCATATGTCGGACCCTAGGGCTAGCTGGCCCTGACGACTTTGCTATTCCGATGGAGGCTTGGGAGGCTAGAAAGGTTCGTTCATCTTCCGAGCTTCTACCGAGGTCGAGGCTGTATCCTATGGATACCTCTCCGAAGACAGAGGAGATTAGTGAGGATAAGGAAGATAAAGAAATTCAAGATGAATTATGTCGGAGGGTTAAGGATTCAGTTCGAATTAGCGTCGACCTTTCCAAGACAAAGACCGAATTTGCAGAGTTGAATGAACGCCGGATGGCTACTGCTACTGGATGTAGTTCGAGAAGTGGAATCAATGGTGCCCGGCCTCCTTTACTTAAGCCGCCGCCGTCCATGCGGCTACCAAATTTTGATAATGCTTACTCTACTTGGGACATTTTGAAAGGTTTTGCTCCATTAGTTGAGGATGAACATCAAGAGGAAGTAGGAGAACGTGTGGAACCTTTAGTTGAAGTAGAAGGAGAAGGAGAAGGAAATACAGTAAGGCCTGTGGAGAACGCATCGCTCATAGGGTCTTGGGGTTCCTTCACGACATCGAATGATGATGATTCTTCAAGCAGTACTACGGAGCCTGCAAATATTTCACCCAATATGAGAGTTAATCCCATTATCACATCCTGGGTTTTAGGCAGGCTTCTTGGGCGCGGTTCATTTGGATCAGTATACGAAGCAATTTCAGAGTACGTAGTGTTTCTTCTACCTTATTTTTGTCGGCAATGTTTGTTTGATTTTCTATGGGGTTCTTGTATATAGGATAAACTTGTCGCACGACTAATATGACTCTGGCATATCTGACCTGGGAATTTTCTGGTGGCTGATCTGATATTGTTTTTGCTGATGGGAATGGGACTCATCCTAAACGGGTCTGAATTTTTTATTAGTTGGAAAACTCATCTGTTTTTATTTCTCATTTTCAGGGATGGGACATTTTTTGCACTCAAAGAAGTTTCACTGCTCGATGAAGATAGTCAGGGAAGACAAAGTATCTATCAACTTCAGCAGGTGATTTACACTTGTTCATGACAATATTTTTGTTATGCTGTAAAGTTCAGGTTCTGTTTGATAGCAACTTTATGTTTTATTTTTATCTTTGTTAAGTAAACACTTAAATTCTTGGCCATATTTAGAAAATGAAATTAGGTTTTTTAAAAACTACTATTTTTAGTTTTTGAAAACTTGTTTTCTTTTTTTGTCTTTTTTTTTTGTTTTGGGAATTGGACTAAGAATTCAAACATTTCTTTGATGACGGCGAAAAAAGTATACTAAAGAAGTAGTTAATTCAAAAACTGAAAACTAAAAACGAAGTTGTTACCAAATGGGTCCTTGTTATGATTTGATATTCTCATCATCTTTTAATGAATGAAGTCTTTATGATCTTATTCTTAATCTGCTTTTCTCACTTCATTTGATTTGTCTTCCAGGAGATAGCTCTTCTAAGTGAGTTTGAGCATGAGAACATAGTTCAGTACTATGGCACACATTCGGTATTGGCCTTGATCCTAAAGAATTGAAATTATTACGCATTTATATATCATCTTTGGATGGTCCGAAAAAAAATATGCTTGATGAATTTAGTTAGATTTACTTTTAGTATTTATAAACTCTCTAGTATCTTGGTTGTAGTCTTTTAGCATATTCTTCATTTGAAATGAAATCCATTTCCTCCAATTCTGATCTTTCTCCTGGCTCTTTGATAACCCAATTCATTTTTAATTACGGTTGAGTAGACACATGGAGTGTTCAAATCAATGTTAGTCTCCACTGGAATCACAAACGGATTTGATACTTGCCTAAATTATTATTTGTACCATGATGCTTATGTTTTCTTTGTTTACTTTTCACCACAATTCCCCCCTTCTCATTGCATCTTCTTTGGCCATAATATTAAACTCAGGATCTCCAATCAATTGTATATGCTTCAAAAGCCTCATGTAATTTTATATAATTTATCTTCCATTGCCTAAAGTTAAGTTTCTACAATTCTTTACAAACTTGTCTGAAGTCAGTTTATTCAATGATAAAACTTACTTTTAGTGCATGCCGATATATGCTTTTCAATGAACCTGAGTGCGCACGCAAAACATAACCACATGTAGAGTCCAAATTCGAAAGTTACTTGGCTATCGTGATCTAAACCATCAACATACAAATTTTACATTGTTTGATACTTTTTTCTGCCAGCATGTGTAAAGTGGTTTGTTATACTAACTTGACATACCAATTGTCCGGCCTCGTCTTATTTTAATTTTCCATATTATTGGAAGTGTGTTGGTAGTTGCACTGATGGGCATTTGGAACGTGTATTATCATGACTGAAAATGAAAAACCACATGTTGGAGCACACATTAATCTAACCAACTTTCCTGCTCTCTTTAGATTTGAGCTTTAAATCAAGATATATTACGGATCCTTGCATGGCTATCTAGAATTTTCATTCATCAATTTTAGCTAATGGGCAATTAATTTACTTTAGGTTTCCTCTCTTTGGGCTAATTGACTAATAAAAATTTGAATATTCAATAGTTAATAATTTATTGATTTTATTTGCAGGATGGGTCAAAACTATATATCTTCCTCGAGCTTGTATCTCAAGGCTCCCTTATGAGTCTATATCAGAGGACAAGCCTTATGGATTCCATTGTTTCTGCCTACACAAGGCAGATCCTATCGGGTTTAAAGTATCTTCACGAGCGAAATGTGATTCACAGGTATACAAATGAATCTATTATGTAATTGTTTATGAATTATGCTTGTGAATGAAGTTGTTCGACATCCTATCCTATCTACCACTTACCAATACTAGACATTTTTATGTACTTTTTTTTAATAATTTATTTGGTTCAGTACACATATCATTATATACTATGATTGTAGCCTGAGACTTCTATGGATCAGTCTGCATTCATTGTGTTCATCTTTAATACCATTGCTGTGGATAGGAAGGGGAAATAAATCTTCAGTTGTCAACTCCATGCTACATTTTTTTTAGTTTTCCTAGTGAAAGAAGAAAACTCTTCACCATTTCTTGCGCCCTCTGATTTTTAGCATTATTTGATCTTCAGTTGGTCATATTGGAAGAAGCTGAATTCAATCCTCCAACAGTTCAAATTCCTTCTGAAATATAATTAACTCAACAGTGTTCTTATTTCTTAACATCCCAGTCTACCCAATTTTCTAAATTAATTTTGGAGATTTAAGAGTCTGTAAAACAGCATAACATAATGTCCTTTATCCATCAATGTTCCCAATGTTTAAAATTAAAACATTTTCTTAGTCGTCATATAGATGAAATCAAACATTTTTGGGGGAAATGAGGGGTGTGAGAGAATAACATTGGCCCACTTATGGGCTTATGGGGTAATTTCTGGTGATTTTTTTCTTGTAATAATGACACCAAATGTATTTTGACCAATCGGACATCTTCTTTTGTAGCCATTTAGTCCTGTGGGGATGTCATCCCCCTCTATCATATTTTCAGTTTTTTTTAAAAAGAATTCCTTGATCTTAAAGCATATGCAACAAATATATGCCCTGTATTGAAAATCTGTAAAATTCTTCTTGATGAGATTACATGTAGATTTTTTCTGTTTGTGTCAAAATAATATATCTCAAAACTTAATTATTTCAAATGTGTTTTTTTTTCATTCCCTTTTTCTTTTTGAGTATTTAATTTTTTTATTGAGAAAAACCTCATATTTATTGCTCTCCTCTTAATGTATAACTATTTTATTTTTTTGATCGGTCGATTTATTTACTTTTTTCATAAAGGGATATTAAATGTGCTAATATATTGGTGGACGTGAATGGATCTGTGAAGCTTGCAGATTTTGGTTTGGCAAAGGTTTTCCCCAATCCACTTTACTATATCTGTTTTTGGTGTTTTTTTCGTGAGTTTCTTCCCTTAAAATCATTGTTTTTATACATTTCAGGCAACAAAATTGAACGATGTTAAATCTTGTAAGGGAACTGCGTATTGGATGGCACCTGAGGTTTGCCTTCTTCCTTGAAGTCTCATGCATCAGAAATATTCTTAATACACGTTCTAGTATTCTCATTCATCGCCCATCTGTTATTATTGTGATTTGTTCAGGTGGTTAATGGGAAGGGTCAAGGATATGGTCTACCTGCCGATATCTGGAGCCTTGGGTGCACAGTATTGGAAATGTTAACCCGAAAGCTTCCATATTCAGAATTTGAATCTGTACGTGTTACCTCTATTCTCCTCTGTCTACAAACTTTGTTTTTGCTGTTGGCTGTATTTCTAAGCTCACAGTTTGAACTGAAACTGAGATAATACAAGCTAAATGGTTTGAAGGTGGCCGAAAACTTGCCACGCTCAGCTTTCTCTTGTTCATCAGTGATTTCAAACTATTTTGTATGGTATGGTTGGAGATAGGACAGCCATTATTTTGTTTCTAAACTGCTTTCTTTTGAATTCAGCATATGCAGGCACTATTTAGGATCGGAAAAGGCAAACCGCCTGCAGTTCCCGAGTCTCTCCCAAAAGATGCACAGGATTTCATTCTGCAGTGCCTACAAGTTAATCCAAAGGACCGTCCTACTGCTGCTGATCTTTTAAATCACTCGTTTGTGAAAAGACCAGTCTCCAGTTTATCAGGGTTGGCATCTCCTTATAACAGGCCAGGCAGGAGGATTTAAACTTGTGAAGAATTGAATTTACAAGGTAGAAATTCTGACTTCGTCTGACACTAAAAATTACCATATTTGCATGTTTGTTATTTGTTCTCTATTTCATTGGGTTGAAATATATATATTATTTCCTATTCTCTCAGTTTCAATTGCTGAAAATAGACATATACGTCCAACCGACATGGTTATGTCTCGTTTAGTTGAATGGCTTGCTGAGGAATCAGGATCCAGTTCTGGTTCTTTGCACAATCTCGGCTGTAAAAGCTTGACTTGAGACATTTTTTTCTGTAATTATTGGAATTCAAGTGTAGAGGTTGATCTATACAATACAAGGGAGGGCAGGCTGGCATTGATGCAAGAATAGTTAAATGGAAGTGCTTCTAGTTATTGTTCGTTAGTTTCTTGGCTGATAACAGAGAACAAGAAGAATGTAATTTTGATGATTTAGCTTATGATTGTTTATTTTAAGTTGGTTTTGTTAATTTGCTTAGCTACTTTGCCTAATTTAGGCTAAGGTGTGTGTAACTGTACAAAAGAAGAAACATTGACACTACTTGTAAAAATTATAATATAGCTACTACGCCTGATATTAGGCTAAGTCACTTCATTTCTTTCCTTCTTTCCTTCTTTTCATTTTAAAAAAATATTTATGTG

Coding sequence (CDS)

ATGAATCGTCTCCCTCAGTTTTTCTCTCCTAGGAACAAAAGAAAACCCATGGATCCCAGGAAAATTCCAGGGAAACCCAAGCTTGGCCGACTTAATGCGGCCAAGAATATTGATTATGATGCTCCTTCCTCGTCTTCTTCGCTCGAAGATTCTTCTGGGTCTCTTTACACTCGTTCCATGGAAAACCCCGATCCCTCCAGTTTTCGGATTAAAGGGCTTGACGGTGAAGTTGATCTCATATGTCGGACCCTAGGGCTAGCTGGCCCTGACGACTTTGCTATTCCGATGGAGGCTTGGGAGGCTAGAAAGGTTCGTTCATCTTCCGAGCTTCTACCGAGGTCGAGGCTGTATCCTATGGATACCTCTCCGAAGACAGAGGAGATTAGTGAGGATAAGGAAGATAAAGAAATTCAAGATGAATTATGTCGGAGGGTTAAGGATTCAGTTCGAATTAGCGTCGACCTTTCCAAGACAAAGACCGAATTTGCAGAGTTGAATGAACGCCGGATGGCTACTGCTACTGGATGTAGTTCGAGAAGTGGAATCAATGGTGCCCGGCCTCCTTTACTTAAGCCGCCGCCGTCCATGCGGCTACCAAATTTTGATAATGCTTACTCTACTTGGGACATTTTGAAAGGTTTTGCTCCATTAGTTGAGGATGAACATCAAGAGGAAGTAGGAGAACGTGTGGAACCTTTAGTTGAAGTAGAAGGAGAAGGAGAAGGAAATACAGTAAGGCCTGTGGAGAACGCATCGCTCATAGGGTCTTGGGGTTCCTTCACGACATCGAATGATGATGATTCTTCAAGCAGTACTACGGAGCCTGCAAATATTTCACCCAATATGAGAGTTAATCCCATTATCACATCCTGGGTTTTAGGCAGGCTTCTTGGGCGCGGTTCATTTGGATCAGTATACGAAGCAATTTCAGAGGATGGGACATTTTTTGCACTCAAAGAAGTTTCACTGCTCGATGAAGATAGTCAGGGAAGACAAAGTATCTATCAACTTCAGCAGGAGATAGCTCTTCTAAGTGAGTTTGAGCATGAGAACATAGTTCAGTACTATGGCACACATTCGGATGGGTCAAAACTATATATCTTCCTCGAGCTTGTATCTCAAGGCTCCCTTATGAGTCTATATCAGAGGACAAGCCTTATGGATTCCATTGTTTCTGCCTACACAAGGCAGATCCTATCGGGTTTAAAGTATCTTCACGAGCGAAATGTGATTCACAGGGATATTAAATGTGCTAATATATTGGTGGACGTGAATGGATCTGTGAAGCTTGCAGATTTTGGTTTGGCAAAGGCAACAAAATTGAACGATGTTAAATCTTGTAAGGGAACTGCGTATTGGATGGCACCTGAGGTGGTTAATGGGAAGGGTCAAGGATATGGTCTACCTGCCGATATCTGGAGCCTTGGGTGCACAGTATTGGAAATGTTAACCCGAAAGCTTCCATATTCAGAATTTGAATCTCATATGCAGGCACTATTTAGGATCGGAAAAGGCAAACCGCCTGCAGTTCCCGAGTCTCTCCCAAAAGATGCACAGGATTTCATTCTGCAGTGCCTACAAGTTAATCCAAAGGACCGTCCTACTGCTGCTGATCTTTTAAATCACTCGTTTGTGAAAAGACCAGTCTCCAGTTTATCAGGGTTGGCATCTCCTTATAACAGGCCAGGCAGGAGGATTTAA

Protein sequence

MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMDTSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHSDGSKLYIFLELVSQGSLMSLYQRTSLMDSIVSAYTRQILSGLKYLHERNVIHRDIKCANILVDVNGSVKLADFGLAKATKLNDVKSCKGTAYWMAPEVVNGKGQGYGLPADIWSLGCTVLEMLTRKLPYSEFESHMQALFRIGKGKPPAVPESLPKDAQDFILQCLQVNPKDRPTAADLLNHSFVKRPVSSLSGLASPYNRPGRRI
Homology
BLAST of Cucsat.G7727 vs. ExPASy Swiss-Prot
Match: Q39008 (Mitogen-activated protein kinase kinase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=MEKK1 PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 5.7e-54
Identity = 157/384 (40.89%), Postives = 207/384 (53.91%), Query Frame = 0

Query: 27  KLGRLNAAKNIDYDAPS-SSSSLED---SSGSLYTRSMENPDPSSFRIKGLDGEVDLICR 86
           +L R +AA+NI+YDA S SSSS ED   S+ SL TRS+E P+P+SFRI G  GE+D I R
Sbjct: 26  RLERRDAARNINYDAASCSSSSAEDLSVSTSSLMTRSLEFPEPTSFRIGGGVGEMDRIYR 85

Query: 87  TLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD---------TSPKTEEISEDKE 146
           +LG++GPDD AI  +AWEA K RSSS+++ R + + +D           P    +  D  
Sbjct: 86  SLGVSGPDDLAISFDAWEACKKRSSSDVVNRFKSFDLDKVRDQDLSEEGPSGVVVGSDSM 145

Query: 147 DKEIQDELCRRVKDSVRISVDLSKTKTEFAEL----------NERRM------------- 206
           + ++Q +       S  I  +LS+       +          N R M             
Sbjct: 146 NHKVQGQDLSEAGPSGGIVTELSEIGNLITPVDRLVADGVVENRRVMERTPTIVKSKGYL 205

Query: 207 ----ATATGCSSRSGINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAP---------- 266
                 A G     GI G RPP+LKPPP+M+ P  D+  S+WD L  FAP          
Sbjct: 206 VPNNVVAVGVGVGGGIKGLRPPVLKPPPAMKRPPIDHRGSSWDFLTHFAPSETVKRPSSS 265

Query: 267 --LVEDEHQEEVGERVEPLVEVEGEGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTE 326
               ED   EE G+      E E E E    R ++         SFTT N+ DSSS+ + 
Sbjct: 266 SSSSEDGCDEEEGK------EEEAEAEEMGARFIQLGDTADETCSFTT-NEGDSSSTVSN 325

Query: 327 PANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSI 359
            + I P+     IITSW  G+LLGRGSFGSVYE IS DG FFA+KEVSLLD+ SQ ++ I
Sbjct: 326 TSPIYPD--GGAIITSWQKGQLLGRGSFGSVYEGISGDGDFFAVKEVSLLDQGSQAQECI 385

BLAST of Cucsat.G7727 vs. ExPASy Swiss-Prot
Match: O81472 (Mitogen-activated protein kinase kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MEKK2 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.4e-36
Identity = 148/457 (32.39%), Postives = 203/457 (44.42%), Query Frame = 0

Query: 27  KLGRLNAA-KNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGL-DGEVDLICRTL 86
           +L R +A  +NIDYDA       EDSS  L TRS++ P+ +SFR+ G+ DGE+D I + +
Sbjct: 123 RLDRSDAVRRNIDYDAG------EDSSSLLITRSLDFPNRTSFRVDGVDDGEIDRIYQYI 182

Query: 87  GLAGPDDFAIPMEAWEAR--KVRSSSELLPRSRLYPMDT--------------------- 146
           G++GP+DFAI  +AW+AR    RSSS+++ + +   +D+                     
Sbjct: 183 GVSGPEDFAISSDAWKARMEHERSSSDVVNKLKSLDLDSREAGPSGGVVASSSMNHKFQG 242

Query: 147 ---------------------SPKTEEIS--EDKE------------------------- 206
                                S K E ++   DKE                         
Sbjct: 243 HDLSEAGSIGVVVASNFTLSESNKIENLNSLRDKEIVDGDMVENRCGIERKPTILVKSRG 302

Query: 207 ---------------------------DKEIQDELCRRVKDSVR-------------ISV 266
                                      DKE+ D      K  +              +S 
Sbjct: 303 YLVHNDDVGVGGGIKGVRPPVLNVPRADKEVVDGGTVESKSGIEWKPTILVKSKGYLVSN 362

Query: 267 DLSKTKTEFAELN----ERRMATATGCSSRSGINGARPPLLKPPPSMRLPNFDNAYSTWD 326
           D          LN    ++ +  +    +R GI G RP +LKPPP M+LP  D   S+WD
Sbjct: 363 DGGIKGVTSPVLNLRPTDKEVVDSGTVENRRGIKGVRPSVLKPPPVMKLPPVDLPGSSWD 422

Query: 327 ILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGNTVRPVENASLI-------GSWGSFTT 359
           IL  FAP  E   +       E   + E E E + V   E   +            SFTT
Sbjct: 423 ILTHFAPDSEIVRRPSSSSSSENGCD-EEEAEDDKVEKEETGDMFIQLEDTTDEACSFTT 482

BLAST of Cucsat.G7727 vs. ExPASy Swiss-Prot
Match: Q9SZ67 (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 6.8e-23
Identity = 78/178 (43.82%), Postives = 97/178 (54.49%), Query Frame = 0

Query: 182  INGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGE 241
            I G RPP+L+  P+M+L +     STWD +  FAP           E  E   EVE E  
Sbjct: 1527 IKGLRPPVLQLQPAMKLSHIPRG-STWDFVTHFAPPETVAPPSSSSEAREE--EVETEET 1586

Query: 242  GNTVRPVENASLIGSWGSFTTSNDDDSSS-STTEPANISPNMRVNPIITSWVLGRLLGRG 301
            G    P+ +        SFT +  D S + S T P   S        IT W  G+LLGRG
Sbjct: 1587 GAMFIPLGDKETC----SFTVNKGDSSRTISNTSPIYASE----GSFITCWQKGQLLGRG 1646

Query: 302  SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT 359
            S GSVYE IS DG FFA KEVSLLD+ SQ  + I Q++  IALLS+ +H+NIV+Y GT
Sbjct: 1647 SLGSVYEGISADGDFFAFKEVSLLDQGSQAHEWIQQVEGGIALLSQLQHQNIVRYRGT 1693

BLAST of Cucsat.G7727 vs. ExPASy Swiss-Prot
Match: Q9M0T3 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MEKK3 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 9.2e-20
Identity = 50/78 (64.10%), Postives = 62/78 (79.49%), Query Frame = 0

Query: 281 NMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQE 340
           N R    ITSW+ G+LLGRGS+ SVYEAISEDG FFA+KEVSLLD+  Q ++ I QL+ E
Sbjct: 293 NFRKPEDITSWLKGQLLGRGSYASVYEAISEDGDFFAVKEVSLLDKGIQAQECIQQLEGE 352

Query: 341 IALLSEFEHENIVQYYGT 359
           IALLS+ +H+NIV+Y GT
Sbjct: 353 IALLSQLQHQNIVRYRGT 370


HSP 2 Score: 52.4 bits (124), Expect = 1.3e-05
Identity = 46/133 (34.59%), Postives = 64/133 (48.12%), Query Frame = 0

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQ-EEVGERVEPLVEVE-G 240
           GI   RPP+++PPP  +LP  D   S+WD L  FAP    + Q     +      EVE  
Sbjct: 30  GIKAVRPPIIQPPPGRKLPLIDFPGSSWDFLTYFAPSKTVKRQSSSSSDNTSDKEEVETE 89

Query: 241 EGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRV-NPIITSWVLGRLL 300
           E  G  V+  + A     +     +N+ DSSS+    + ISP+      I+ SW+  + L
Sbjct: 90  ETRGMFVQLGDTAHEACPF----ATNEADSSSTV---SIISPSYASRGSIVPSWLKRKFL 149

Query: 301 GRGSFGSVYEAIS 311
           GR S G VYE  S
Sbjct: 150 GRVSLGFVYEGSS 155

BLAST of Cucsat.G7727 vs. ExPASy Swiss-Prot
Match: A9SY39 (Mitogen-activated protein kinase kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MEKK1a PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 4.3e-17
Identity = 47/91 (51.65%), Postives = 62/91 (68.13%), Query Frame = 0

Query: 268 SSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGTFFALKEVSLLDED 327
           SS ST    ++ P     P   +W  G  LG G+FGSVYE ++ +GTFFA+KEV+L DE 
Sbjct: 406 SSVSTESSPSVIPK---RPPWDTWAKGEFLGSGTFGSVYEGVARNGTFFAVKEVNLADEG 465

Query: 328 SQGRQSIYQLQQEIALLSEFEHENIVQYYGT 359
             GRQ++ QL++EIALLS+ +H NIVQY GT
Sbjct: 466 KLGRQAVKQLEREIALLSDIQHPNIVQYLGT 493

BLAST of Cucsat.G7727 vs. NCBI nr
Match: XP_011655187.1 (mitogen-activated protein kinase kinase kinase 1 [Cucumis sativus] >KGN50986.1 hypothetical protein Csa_008947 [Cucumis sativus])

HSP 1 Score: 698 bits (1802), Expect = 8.44e-250
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
           TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS
Sbjct: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG 300
           EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG
Sbjct: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG 300

Query: 301 SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360

BLAST of Cucsat.G7727 vs. NCBI nr
Match: XP_008460456.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo])

HSP 1 Score: 678 bits (1749), Expect = 1.05e-241
Identity = 354/362 (97.79%), Postives = 356/362 (98.34%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MD
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
           TSPKTEEIS+DKEDKEIQDELCRRVKDSVRISV+LSKTKTEFAELNERRMATATGCSSRS
Sbjct: 121 TSPKTEEISKDKEDKEIQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EG--NTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLG 300
           EG  NTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLG
Sbjct: 241 EGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLG 300

Query: 301 RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT 360
           RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT
Sbjct: 301 RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT 360

BLAST of Cucsat.G7727 vs. NCBI nr
Match: TYK26507.1 (mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 646 bits (1666), Expect = 2.48e-229
Identity = 338/346 (97.69%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 17  MDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 76
           MDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE
Sbjct: 1   MDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 60

Query: 77  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMDTSPKTEEISEDKEDKE 136
           VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MDTSPKTEEIS+DKEDKE
Sbjct: 61  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMDTSPKTEEISKDKEDKE 120

Query: 137 IQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 196
           IQDELCRRVKDSVRISV+LSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM
Sbjct: 121 IQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 180

Query: 197 RLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG--NTVRPVENASLI 256
           RLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG  NTVRPVENASLI
Sbjct: 181 RLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGEGNTVRPVENASLI 240

Query: 257 GSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGT 316
           GSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLGRGSFGSVYEAISEDGT
Sbjct: 241 GSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLGRGSFGSVYEAISEDGT 300

Query: 317 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 346

BLAST of Cucsat.G7727 vs. NCBI nr
Match: KAA0067367.1 (mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 644 bits (1661), Expect = 1.43e-228
Identity = 337/346 (97.40%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 17  MDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 76
           MDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE
Sbjct: 1   MDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 60

Query: 77  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMDTSPKTEEISEDKEDKE 136
           VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MDTSPKTEEIS+DKEDKE
Sbjct: 61  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMDTSPKTEEISKDKEDKE 120

Query: 137 IQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 196
           IQDELCRRVK+SVRISV+LSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM
Sbjct: 121 IQDELCRRVKNSVRISVELSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 180

Query: 197 RLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG--NTVRPVENASLI 256
           RLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG  NTVRPVENASLI
Sbjct: 181 RLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGEGNTVRPVENASLI 240

Query: 257 GSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGT 316
           GSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLGRGSFGSVYEAISEDGT
Sbjct: 241 GSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLGRGSFGSVYEAISEDGT 300

Query: 317 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 346

BLAST of Cucsat.G7727 vs. NCBI nr
Match: XP_038874370.1 (mitogen-activated protein kinase kinase kinase 1-like [Benincasa hispida])

HSP 1 Score: 635 bits (1639), Expect = 4.83e-225
Identity = 331/360 (91.94%), Postives = 342/360 (95.00%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           M+RLPQFFSPR K KPMDPRKI GKPKLGRLNAAKNIDYDA SSSSSL+DSSGSLYTRSM
Sbjct: 1   MHRLPQFFSPRKKGKPMDPRKIAGKPKLGRLNAAKNIDYDAASSSSSLDDSSGSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           E PDPSSFRIKGLDGEVDLICRTLGL GPDDFAIPMEAWEARKVRSSSELLPRSR+YPMD
Sbjct: 61  ETPDPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSELLPRSRMYPMD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
           +SPKTEEI++DKEDKEIQDELCRRVKDSVRISV+LS  KTEFAELNERRMATATGCSSRS
Sbjct: 121 SSPKTEEINKDKEDKEIQDELCRRVKDSVRISVELSTPKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLPN DN  STWDILK FAPLV+DEHQEEVGERVEPLV+   EG
Sbjct: 181 GINGARPPLLKPPPSMRLPNLDNTCSTWDILKSFAPLVDDEHQEEVGERVEPLVD--REG 240

Query: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG 300
           EGN VRPVENA LIGSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWV+GRLLGRG
Sbjct: 241 EGNIVRPVENALLIGSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVVGRLLGRG 300

Query: 301 SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           SFGSVYEAIS+DGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 SFGSVYEAISQDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 358

BLAST of Cucsat.G7727 vs. ExPASy TrEMBL
Match: A0A0A0KTG3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G385380 PE=4 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 4.09e-250
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
           TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS
Sbjct: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG 300
           EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG
Sbjct: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRG 300

Query: 301 SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 SFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360

BLAST of Cucsat.G7727 vs. ExPASy TrEMBL
Match: A0A1S3CCL9 (mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103499270 PE=4 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 5.07e-242
Identity = 354/362 (97.79%), Postives = 356/362 (98.34%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSM
Sbjct: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MD
Sbjct: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
           TSPKTEEIS+DKEDKEIQDELCRRVKDSVRISV+LSKTKTEFAELNERRMATATGCSSRS
Sbjct: 121 TSPKTEEISKDKEDKEIQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG
Sbjct: 181 GINGARPPLLKPPPSMRLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240

Query: 241 EG--NTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLG 300
           EG  NTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLG
Sbjct: 241 EGEGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLG 300

Query: 301 RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT 360
           RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT
Sbjct: 301 RGSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGT 360

BLAST of Cucsat.G7727 vs. ExPASy TrEMBL
Match: A0A5D3DRZ7 (Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00830 PE=4 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 1.20e-229
Identity = 338/346 (97.69%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 17  MDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 76
           MDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE
Sbjct: 1   MDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 60

Query: 77  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMDTSPKTEEISEDKEDKE 136
           VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MDTSPKTEEIS+DKEDKE
Sbjct: 61  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMDTSPKTEEISKDKEDKE 120

Query: 137 IQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 196
           IQDELCRRVKDSVRISV+LSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM
Sbjct: 121 IQDELCRRVKDSVRISVELSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 180

Query: 197 RLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG--NTVRPVENASLI 256
           RLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG  NTVRPVENASLI
Sbjct: 181 RLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGEGNTVRPVENASLI 240

Query: 257 GSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGT 316
           GSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLGRGSFGSVYEAISEDGT
Sbjct: 241 GSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLGRGSFGSVYEAISEDGT 300

Query: 317 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 346

BLAST of Cucsat.G7727 vs. ExPASy TrEMBL
Match: A0A5A7VJL0 (Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold40G00080 PE=4 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 6.92e-229
Identity = 337/346 (97.40%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 17  MDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 76
           MDPRKIPGKPKLGRLNAAKNIDYDA SSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE
Sbjct: 1   MDPRKIPGKPKLGRLNAAKNIDYDAASSSSSLEDSSGSLYTRSMENPDPSSFRIKGLDGE 60

Query: 77  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMDTSPKTEEISEDKEDKE 136
           VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLY MDTSPKTEEIS+DKEDKE
Sbjct: 61  VDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYSMDTSPKTEEISKDKEDKE 120

Query: 137 IQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 196
           IQDELCRRVK+SVRISV+LSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM
Sbjct: 121 IQDELCRRVKNSVRISVELSKTKTEFAELNERRMATATGCSSRSGINGARPPLLKPPPSM 180

Query: 197 RLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG--NTVRPVENASLI 256
           RLPNFDNA STWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEG  NTVRPVENASLI
Sbjct: 181 RLPNFDNACSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEGEGEGNTVRPVENASLI 240

Query: 257 GSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWVLGRLLGRGSFGSVYEAISEDGT 316
           GSWGSFTTSNDDDSSSSTTEPANISPNMR NPIITSWVLGRLLGRGSFGSVYEAISEDGT
Sbjct: 241 GSWGSFTTSNDDDSSSSTTEPANISPNMRGNPIITSWVLGRLLGRGSFGSVYEAISEDGT 300

Query: 317 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 360
           FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS
Sbjct: 301 FFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTHS 346

BLAST of Cucsat.G7727 vs. ExPASy TrEMBL
Match: A0A6J1JRP4 (mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489185 PE=4 SV=1)

HSP 1 Score: 545 bits (1404), Expect = 1.16e-189
Identity = 297/361 (82.27%), Postives = 316/361 (87.53%), Query Frame = 0

Query: 1   MNRLPQFFSPRNKRKPMDPRKIPGKPKLGRLNAAKNIDYDAPSSSSSLEDSSGSLYTRSM 60
           M+ LPQFFSPR + K MDPRKI GKPKL RLNAAKNIDYDA  S SS +DSS SLYTRSM
Sbjct: 1   MHHLPQFFSPRKRSKAMDPRKIAGKPKLYRLNAAKNIDYDAAYSYSSFDDSSRSLYTRSM 60

Query: 61  ENPDPSSFRIKGLDGEVDLICRTLGLAGPDDFAIPMEAWEARKVRSSSELLPRSRLYPMD 120
           E  +PSSFRIKGLDGEVDLICRTLGL GPDDFAIPMEAWEARKVRSSS++LPRSRLYP+D
Sbjct: 61  ETFNPSSFRIKGLDGEVDLICRTLGLDGPDDFAIPMEAWEARKVRSSSDILPRSRLYPVD 120

Query: 121 TSPKTEEISEDKEDKEIQDELCRRVKDSVRISVDLSKTKTEFAELNERRMATATGCSSRS 180
            SPKTEEIS+ KEDKE +DELCRRVKDSVRIS ++S  KTE  ELNERRM T TG S RS
Sbjct: 121 -SPKTEEISKGKEDKENEDELCRRVKDSVRISDEMSTLKTELPELNERRMVTVTGRSPRS 180

Query: 181 GINGARPPLLKPPPSMRLPNFDNAYSTWDILKGFAPLVEDEHQEEVGERVEPLVEVEGEG 240
           GINGARPPLLKPPPSMRLP FDNA+STWDILK FAPLV+D+ Q+EV ERVEPLVE   E 
Sbjct: 181 GINGARPPLLKPPPSMRLPVFDNAFSTWDILKAFAPLVDDKKQDEVEERVEPLVE--REV 240

Query: 241 EGNTVRPVENASLIGSWGSFTTSNDDDSSSSTTEPANISPNMRVNPIITSWV-LGRLLGR 300
           E N VRP E+A+LI SWGSFTTSNDDDSSSSTTEPANISPNMR  PIITSWV  GRLLGR
Sbjct: 241 EENIVRPEESAALIESWGSFTTSNDDDSSSSTTEPANISPNMRSKPIITSWVETGRLLGR 300

Query: 301 GSFGSVYEAISEDGTFFALKEVSLLDEDSQGRQSIYQLQQEIALLSEFEHENIVQYYGTH 360
           GSFGSV EA+SEDG FFALKEVSLLDEDSQGRQSI QLQQEI+LLSEFEHENIVQYYGTH
Sbjct: 301 GSFGSVSEALSEDGIFFALKEVSLLDEDSQGRQSILQLQQEISLLSEFEHENIVQYYGTH 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q390085.7e-5440.89Mitogen-activated protein kinase kinase kinase 1 OS=Arabidopsis thaliana OX=3702... [more]
O814725.4e-3632.39Mitogen-activated protein kinase kinase kinase 9 OS=Arabidopsis thaliana OX=3702... [more]
Q9SZ676.8e-2343.82Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 ... [more]
Q9M0T39.2e-2064.10Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
A9SY394.3e-1751.65Mitogen-activated protein kinase kinase kinase 1a OS=Physcomitrium patens OX=321... [more]
Match NameE-valueIdentityDescription
XP_011655187.18.44e-250100.00mitogen-activated protein kinase kinase kinase 1 [Cucumis sativus] >KGN50986.1 h... [more]
XP_008460456.11.05e-24197.79PREDICTED: mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo][more]
TYK26507.12.48e-22997.69mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo var. makuwa][more]
KAA0067367.11.43e-22897.40mitogen-activated protein kinase kinase kinase 1-like [Cucumis melo var. makuwa][more]
XP_038874370.14.83e-22591.94mitogen-activated protein kinase kinase kinase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KTG34.09e-250100.00Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G385... [more]
A0A1S3CCL95.07e-24297.79mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo OX=3656 GN... [more]
A0A5D3DRZ71.20e-22997.69Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuw... [more]
A0A5A7VJL06.92e-22997.40Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuw... [more]
A0A6J1JRP41.16e-18982.27mitogen-activated protein kinase kinase kinase 1-like OS=Cucurbita maxima OX=366... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 288..341
e-value: 2.5E-5
score: 26.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..279
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..69
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 118..341
coord: 25..128
NoneNo IPR availablePANTHERPTHR48016:SF33SERINE/THREONINE/DUAL SPECIFICITY PROTEIN KINASE, CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 118..341
coord: 25..128
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 297..319
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 291..345
score: 8.41469
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 269..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G7727.T1Cucsat.G7727.T1mRNA
Cucsat.G7727.T2Cucsat.G7727.T2mRNA
Cucsat.G7727.T3Cucsat.G7727.T3mRNA
Cucsat.G7727.T4Cucsat.G7727.T4mRNA
Cucsat.G7727.T6Cucsat.G7727.T6mRNA
Cucsat.G7727.T7Cucsat.G7727.T7mRNA
Cucsat.G7727.T16Cucsat.G7727.T16mRNA
Cucsat.G7727.T12Cucsat.G7727.T12mRNA
Cucsat.G7727.T10Cucsat.G7727.T10mRNA
Cucsat.G7727.T14Cucsat.G7727.T14mRNA
Cucsat.G7727.T15Cucsat.G7727.T15mRNA
Cucsat.G7727.T8Cucsat.G7727.T8mRNA
Cucsat.G7727.T9Cucsat.G7727.T9mRNA
Cucsat.G7727.T5Cucsat.G7727.T5mRNA
Cucsat.G7727.T13Cucsat.G7727.T13mRNA
Cucsat.G7727.T11Cucsat.G7727.T11mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity