Cucsat.G7708 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G7708
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Nitrate reductase
Location	ctg1546: 61126 .. 63358 (+)
RNA-Seq Expression	Cucsat.G7708
Synteny	Cucsat.G7708

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATCTTAATTGGAATCTCATGGTAATTATTAATAATTATGTTTCTATTTCTACATTAAAAATATATTATAGTTAATGAAGTTATTATCGTCAGAAAAATCTTACAAAATCTATGACCCATGCACACATATGAAACTAAAACAATAAAATTTTAACTAAACCATAAGAAACCTGACATATAATGTCCCTTTCTTCAGCCTTCTTCAACTACCTATTTTTTATTCCATCTTTTAAAACACAGATATTTCTTCTTCTTCCCACCTTTTAGCTTGACTTTCCTATTTCTCACCCTCTCATCCATATGATTCAAAGAAAAAGTTTCTTATAAAATAATTATATTTAAACTGCAATATGTATAGTGAGACTCAATGGTTTGATAAATTAAAGAAAACACACGGTTTTTGCAATAAAATATACCATAAAAATATACCATAAAAATATACCCAGGTTGAATAATATAATAAATAATTGATAACATTATAAAATAGTGACATAAATGGGAAGAATGAGAACATTTTATCGTAATAAAAAGTTTATGTAAGGATGACGCAAATTAAATAACCGGTCAATTTGAGAAAATAAAAATAAATATATGTACAAATGTAAACCCTTTTTTGTATGGTGATAGTAGAATACATATTAATTCTACTTTATTTCAAACAATAATACTCGATCAATACATACTGCCAAAAATATTGTTTTTTCTTAAAGAAAAAAGTACCAAAATATTATATTTTTTATGTACGACTTCAACGTTATCATACAAAAATTTATGAACTCTCATAATTAATATAGTAATAATAGTCCTAGAGAAAGAATCATAAGAGAAGAGATGTTGCGGAAACGTACATTCGGTTTTAGTAGTTTTTTAGTTTTTTTCGTGGGTTTTTTCTACAGTTTTTATTTTTTTTAAACTTTTGAAAAACCCCTTTTTATTAAAAAAGATAAATAATAATAATAATTTTTCATAGAGGAATACTATATGAGAACACGTTTTCTACCACAGCTTGTTGATAATAAAAGTACAAAAATGTTTGGATGCAGGGGATGATGAACAACTCATGGTACCGAATCAAGATAAACATATGCAAACCCCAAAAAGGAGAGATCGGGATCGTGTTCCGCCACCCAACAGTCGCCGGCAACAATGCCGGCGGCTGGCAGGAAAAGGACAAACCCAATCCGACCGACGAGTCCCCTTCCCAACAAAAAGACAACAAGAGCACCACCACCTTCTCCATGTCGGAAGTCAAAACCCACAACTCGTCCGAATCGGCTTGGATCGTCGTCCAGAACCATGTCTACGACTGTACTCCTTTTCTCAAAGACCATCCAGGCGGCGCAGACAGCATTCTCATCAACGCTGGCACAGATTGCACGGAAGAGTTCGAGGCTATCCACTCCGACAAAGCCATTAAAATGCTTGAGGACTACAAAATCGGAGACTTGTTGATCCTAAACGACAACAAGGACGCAACGTCATCACCCAAAGCAGTTACTCAACACGTGGCTCTGATCCCTCGTGAGAAAATCCCGGTGAAATTGATTTCGAAAACTACAATTTCTCACAACACGAGAATTTTCAGATTTGGTCTTTCTTCGGAAGATCAAAGATTAGGACTACCCGTTGGAAAGCACATATTTTTATGCGCCAATGTGGATGGGAAACTATGTATGAGAGCCTACACACCGTCAAGCGCCGTTGACCAAACGGGCTACTTCGACTTAGTCGTAAAAATCTATTTCAAGAACGTTCATCCCGATTTCCCCAACGGTGGCCTTATGTCACAGTATCTTGACTCGCTCCCGTTGGGATCGATGGTGGAAGTGAAAGGCCCATTGGGCCATATAGAGTACATGGGGCGCGGCAACTTCAGAGTCAACGGAAAGCCCAGATTGGCCAAGAAGCTTGCCATGGTGGCAGGTGGGACTGGGATAACGCCTATATATCAAGTGGTGCAGGCGATTTTAAAAGATCCGGAGGACGAGACGGAGATGTTTGTTGTATATGCGAACCGGAGTGAGGAGGATATTCTGCTTAGGGAAGAGTTGGAAACATGGGCGAAGGAGAATGAGAGATTGAAGATTTGGTATGTGGTGAAGGATGGGGTTCAAGAAGGGTGGCCGTACAGTGTGGGATATATTAGGGAAGATATTCTGAGGGAGCATATTCCGATGGGGTCACCACAGACTTTGGCTTTGGTCTGTGGGCCGCCTCCCATGATTGAGCTC

Coding sequence (CDS)

AATCTTAATTGGAATCTCATGGGGATGATGAACAACTCATGGTACCGAATCAAGATAAACATATGCAAACCCCAAAAAGGAGAGATCGGGATCGTGTTCCGCCACCCAACAGTCGCCGGCAACAATGCCGGCGGCTGGCAGGAAAAGGACAAACCCAATCCGACCGACGAGTCCCCTTCCCAACAAAAAGACAACAAGAGCACCACCACCTTCTCCATGTCGGAAGTCAAAACCCACAACTCGTCCGAATCGGCTTGGATCGTCGTCCAGAACCATGTCTACGACTGTACTCCTTTTCTCAAAGACCATCCAGGCGGCGCAGACAGCATTCTCATCAACGCTGGCACAGATTGCACGGAAGAGTTCGAGGCTATCCACTCCGACAAAGCCATTAAAATGCTTGAGGACTACAAAATCGGAGACTTGTTGATCCTAAACGACAACAAGGACGCAACGTCATCACCCAAAGCAGTTACTCAACACGTGGCTCTGATCCCTCGTGAGAAAATCCCGGTGAAATTGATTTCGAAAACTACAATTTCTCACAACACGAGAATTTTCAGATTTGGTCTTTCTTCGGAAGATCAAAGATTAGGACTACCCGTTGGAAAGCACATATTTTTATGCGCCAATGTGGATGGGAAACTATGTATGAGAGCCTACACACCGTCAAGCGCCGTTGACCAAACGGGCTACTTCGACTTAGTCGTAAAAATCTATTTCAAGAACGTTCATCCCGATTTCCCCAACGGTGGCCTTATGTCACAGTATCTTGACTCGCTCCCGTTGGGATCGATGGTGGAAGTGAAAGGCCCATTGGGCCATATAGAGTACATGGGGCGCGGCAACTTCAGAGTCAACGGAAAGCCCAGATTGGCCAAGAAGCTTGCCATGGTGGCAGGTGGGACTGGGATAACGCCTATATATCAAGTGGTGCAGGCGATTTTAAAAGATCCGGAGGACGAGACGGAGATGTTTGTTGTATATGCGAACCGGAGTGAGGAGGATATTCTGCTTAGGGAAGAGTTGGAAACATGGGCGAAGGAGAATGAGAGATTGAAGATTTGGTATGTGGTGAAGGATGGGGTTCAAGAAGGGTGGCCGTACAGTGTGGGATATATTAGGGAAGATATTCTGAGGGAGCATATTCCGATGGGGTCACCACAGACTTTGGCTTTGGTCTGTGGGCCGCCTCCCATGATTGAGCTC

Protein sequence

NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPSQQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL

Homology

[Close]

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot
Match: P39865 (Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris OX=3885 GN=NIA1 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 4.6e-166
Identity = 276/420 (65.71%), Postives = 341/420 (81.19%), Query Frame = 0


Query: 2   LNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGW--QEKDKPNPTDESP 61
           L WN MGM+NN W+R+K N+CKP+KGEIGIVF HPT  GN +GGW  +EK     ++ +P
Sbjct: 441 LIWNTMGMINNCWFRVKTNVCKPKKGEIGIVFEHPTQPGNQSGGWMAREKQLEKSSESNP 500

Query: 62  SQQKD------NKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILIN 121
             +K       N +T ++S+SEV+ HN+ +SAWI+V  HVYDCT FLKDHPGG DSIL+N
Sbjct: 501 ILKKSVSSPFMNTATKSYSLSEVRRHNNRDSAWIIVNGHVYDCTRFLKDHPGGEDSILLN 560

Query: 122 AGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAV-----TQH------V 181
           AGTDCTEEFEAIHSDKA KMLEDY+IG+L+  +   D++SS  +V     T H      V
Sbjct: 561 AGTDCTEEFEAIHSDKAKKMLEDYRIGELMTTDYTSDSSSSNNSVHGNSETTHLAPIREV 620

Query: 182 ALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYT 241
           AL PREKIP KL+SKT+ISH+ R+ RF L +EDQ +GLPVG H+FLCA VD KLCMRAYT
Sbjct: 621 ALNPREKIPCKLLSKTSISHDVRLLRFALPAEDQVMGLPVGNHVFLCATVDEKLCMRAYT 680

Query: 242 PSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRG 301
           P+S+VD+ G+FDLVVK+YFK VHP+FPNGG+MSQ+LDSLP+GS+V+VKGPLGHIEY GRG
Sbjct: 681 PTSSVDEVGFFDLVVKVYFKGVHPNFPNGGIMSQHLDSLPIGSVVDVKGPLGHIEYTGRG 740

Query: 302 NFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREE 361
           NF V+GKPR AK+L M+AGGTGITPIYQVVQAILKDPED TEM+VVYANR+E+DILL+EE
Sbjct: 741 NFLVHGKPRFAKRLTMLAGGTGITPIYQVVQAILKDPEDRTEMYVVYANRTEDDILLKEE 800

Query: 362 LETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIE 403
           L+ WAK+++RLK+WYV++  ++EGW YSVG+I E ILREH+P+ SP TLAL CGPPPMI+
Sbjct: 801 LDEWAKKHDRLKVWYVLQANIREGWEYSVGFITESILREHVPLASPDTLALTCGPPPMIQ 860

[Close]

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 2.5e-164
Identity = 279/429 (65.03%), Postives = 341/429 (79.49%), Query Frame = 0


Query: 2   LNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDK-------PNP 61
           L WNLMGMMNN W+R+K N+CKP KGEIGIVF HPT  GN +GGW ++++        N 
Sbjct: 469 LIWNLMGMMNNCWFRVKTNMCKPHKGEIGIVFEHPTQPGNQSGGWMDRERHLEISTESNQ 528

Query: 62  T-DESPSQQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILIN 121
           T  +S S    N ++ T+++SEVK HNS +SAWI+V  HVYDCT FLKDHPGG+DSILIN
Sbjct: 529 TLKKSVSTPFMNTASNTYTLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILIN 588

Query: 122 AGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATS-SPK---------------- 181
           AGTDCTEEF+AIHSDKA KMLEDY+IG+L+      D++S SP                 
Sbjct: 589 AGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIR 648

Query: 182 --AVTQHVALIPREKIPVKLISKTTISHNTRIFRFGL-SSEDQRLGLPVGKHIFLCANVD 241
              V++ VAL P EKIP KLISKT+ISH+ R+FRF L   +DQ LGLPVGKHIF+CA VD
Sbjct: 649 EAPVSRRVALAPNEKIPCKLISKTSISHDVRVFRFALPGGQDQALGLPVGKHIFICATVD 708

Query: 242 GKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPL 301
           GKLCMRAYTP+S++D+ G+F+LVVK+YFK VHP FPNGG+MSQYLDS+ +GS ++VKGPL
Sbjct: 709 GKLCMRAYTPTSSIDEMGFFELVVKVYFKGVHPKFPNGGIMSQYLDSMEVGSTLDVKGPL 768

Query: 302 GHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRS 361
           GHIEY GRGNF V+GKPR A++LAM+AGGTGITPIYQVVQAILKDPEDETEM+VVYANR+
Sbjct: 769 GHIEYTGRGNFMVHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRT 828

Query: 362 EEDILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLAL 403
           E+DILLR+EL+TWAK+N+RLK+WYVV++ ++EGW YSVG+I E+ILREHIP  +  TLAL
Sbjct: 829 EDDILLRDELDTWAKKNQRLKVWYVVQESIREGWEYSVGFITENILREHIPAAAEDTLAL 888

[Close]

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 3.7e-163
Identity = 279/424 (65.80%), Postives = 334/424 (78.77%), Query Frame = 0


Query: 2   LNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGW--QEKDKPNPTDESP 61
           L WN+MGMMNN W+R+K N+CK   GEIGI F HPTV GN +GGW  +EK+    +D + 
Sbjct: 454 LIWNVMGMMNNCWFRVKTNVCKAHMGEIGIAFEHPTVPGNESGGWMAREKNLETSSDANQ 513

Query: 62  SQQKD------NKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILIN 121
           S +K       N S+  FSMSEVK HNS+ESAWI+V  H+YDCT FLKDHPGGADSILIN
Sbjct: 514 SLKKSVSSPFMNTSSKMFSMSEVKKHNSAESAWIIVHGHIYDCTHFLKDHPGGADSILIN 573

Query: 122 AGTDCTEEFEAIHSDKAIKMLEDYKIGDLL---ILNDNKDATSSPKAVTQH--------- 181
           AGTDCTEEF+AIHSDKA KMLEDY+IG+L+    ++D+ ++T    + T H         
Sbjct: 574 AGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYVSDSPNSTVHGASNTSHLAPIKEIAP 633

Query: 182 ---VALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCM 241
              VALIP  KIP KL+ K ++SH+ R+FR  L S+DQ LGLPVGKH+FLCA +D KLCM
Sbjct: 634 LRNVALIPGAKIPTKLVYKKSLSHDVRLFRLALPSDDQVLGLPVGKHVFLCATIDDKLCM 693

Query: 242 RAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEY 301
           RAYTP+S +D+ GY DLVVKIYFKN +P FPNGGLMSQ+LDSLP+GS++ VKGPLGH+EY
Sbjct: 694 RAYTPTSTIDEVGYLDLVVKIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVKGPLGHVEY 753

Query: 302 MGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDIL 361
            GRGNF V+G+P+ AK+LAMVAGGTGITPIYQV+QAILKDPEDETEMFVVYANR+E+DIL
Sbjct: 754 TGRGNFLVHGEPKFAKRLAMVAGGTGITPIYQVIQAILKDPEDETEMFVVYANRTEDDIL 813

Query: 362 LREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPP 403
           LREEL+ WAK++E+LK+WYVVK+  +EGW YSVGYIRE ILREHIP GS   LAL CG P
Sbjct: 814 LREELDDWAKKHEKLKVWYVVKESKREGWEYSVGYIRESILREHIPEGSDDVLALACGAP 873

[Close]

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot
Match: P39869 (Nitrate reductase [NADH] OS=Lotus japonicus OX=34305 GN=NIA PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 9.0e-162
Identity = 281/436 (64.45%), Postives = 329/436 (75.46%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKP---NPTDE 60
           NL WN+MGMMNN W+R+K N+CKP KGEIGIVF HPT  GN  GGW  K+K    +  D 
Sbjct: 445 NLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNQPGGWMAKEKHLEISQQDS 504

Query: 61  SPSQQKD------NKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSIL 120
            P  +K       N  T  +S+SEVK HNS +SAWI+V  HVYDCT FLKDHPGGADSIL
Sbjct: 505 RPILKKSVSSPFMNTFTKMYSLSEVKKHNSPDSAWIIVHGHVYDCTRFLKDHPGGADSIL 564

Query: 121 INAGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSS----------------- 180
           INAGTDCTEEFEAIHSDKA KMLEDY++G+L+      D++S                  
Sbjct: 565 INAGTDCTEEFEAIHSDKAKKMLEDYRVGELITTGYTSDSSSPNNSLHGNSEFKHLAPIK 624

Query: 181 -------PKAVTQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHIF 240
                  P    + VALIPREKIP KLIS+T+ISH+ R+FRF L SEDQ+LGLPVGKHIF
Sbjct: 625 EITTMSLPPLPRRKVALIPREKIPCKLISRTSISHDVRVFRFALPSEDQQLGLPVGKHIF 684

Query: 241 LCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMV 300
           LCA VDGKLCMRAYTP+S VD+ GYF+LVVK+YFK VHP FPNGG MSQ+LDSLP+GS +
Sbjct: 685 LCATVDGKLCMRAYTPTSGVDEVGYFELVVKVYFKGVHPKFPNGGAMSQHLDSLPIGSDL 744

Query: 301 EVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFV 360
           +VKGPLGHIEY GRGNF V+GK R AKKLAM+AGGTGITPIYQV QAILKDPED T+M+V
Sbjct: 745 DVKGPLGHIEYTGRGNFLVHGKHRFAKKLAMLAGGTGITPIYQVAQAILKDPEDHTKMYV 804

Query: 361 VYANRSEEDILLREELETWAKENE-RLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMG 403
           VYANR+E+DILLREEL+TWAK+ E R K+WYVV +  +EGW YSVG++ E ++REH+P  
Sbjct: 805 VYANRTEDDILLREELDTWAKKYEDRFKVWYVV-ETAKEGWGYSVGFVTEGVMREHLPEA 864

[Close]

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot
Match: P39870 (Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.2e-161
Identity = 277/427 (64.87%), Postives = 333/427 (77.99%), Query Frame = 0


Query: 2   LNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDK-------PNP 61
           L WN+MGMMNN W+R+K N+C+P KGEIGIVF HPT  GN +GGW  K+K        NP
Sbjct: 443 LIWNVMGMMNNCWFRVKTNVCRPHKGEIGIVFEHPTQPGNQSGGWMAKEKHLEKSSESNP 502

Query: 62  T-DESPSQQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILIN 121
           T  +S S    N ++ T++MSEV+ HN+++SAWI+V  HVYDCT FLKDHPGG DSILIN
Sbjct: 503 TLKKSVSSPFMNTTSKTYTMSEVRRHNNADSAWIIVHGHVYDCTRFLKDHPGGTDSILIN 562

Query: 122 AGTDCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAV--------------- 181
           AGTDCTEEFEAIHSDKA +MLEDY+IG+L     N D++SS  +V               
Sbjct: 563 AGTDCTEEFEAIHSDKAKQMLEDYRIGELTTTCYNSDSSSSNPSVHGSSDTIPLTPIKEV 622

Query: 182 ---TQHVALIPREKIPVKLISKTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGK 241
               + VAL PREKIP KLISKT+ISH+ R+FRF L S+D  +GLPVGKHIFLCA VD K
Sbjct: 623 ITPMRSVALNPREKIPCKLISKTSISHDVRLFRFALPSDDLLMGLPVGKHIFLCATVDEK 682

Query: 242 LCMRAYTPSSAVDQTGYFDLVVKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGH 301
           LCMRAYTP+S+V + GYFDLVVK+YFK VHP FP GG+MSQ+LDSLP+GS+++VKGPLGH
Sbjct: 683 LCMRAYTPTSSVHEVGYFDLVVKVYFKGVHPKFPTGGIMSQHLDSLPIGSVLDVKGPLGH 742

Query: 302 IEYMGRGNFRVNGKPRLAKKLAMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEE 361
           I Y GRGNF V+GKPR A +LAM+AGGTGITPIYQVV+AILKDPED TEM VVYANR+E+
Sbjct: 743 IVYTGRGNFLVHGKPRFATRLAMLAGGTGITPIYQVVRAILKDPEDCTEMHVVYANRTED 802

Query: 362 DILLREELETWAKENERLKIWYVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVC 403
           DILL+EEL+ WAK+ +RLK+WYV++  ++EGW YSVG+I E IL EHIP  SP TLAL C
Sbjct: 803 DILLKEELDEWAKKYDRLKVWYVIQASIREGWEYSVGFITESILTEHIPNASPDTLALTC 862

[Close]

BLAST of Cucsat.G7708 vs. NCBI nr
Match: NP_001267688.1 (nitrate reductase [NAD(P)H]-like [Cucumis sativus] >AFH74423.1 cucumber nitrate reductase 3 [Cucumis sativus] >KGN50981.1 hypothetical protein Csa_008642 [Cucumis sativus])

HSP 1 Score: 820 bits (2117), Expect = 2.17e-292
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS
Sbjct: 451 NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 510

Query: 61  QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 120
           QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE
Sbjct: 511 QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 570

Query: 121 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 180
           EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI
Sbjct: 571 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 630

Query: 181 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 240
           SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY
Sbjct: 631 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 690

Query: 241 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 300
           FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA
Sbjct: 691 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 750

Query: 301 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 360
           GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK
Sbjct: 751 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 810

Query: 361 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL
Sbjct: 811 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 853

[Close]

BLAST of Cucsat.G7708 vs. NCBI nr
Match: KAE8652087.1 (hypothetical protein Csa_018542 [Cucumis sativus])

HSP 1 Score: 820 bits (2117), Expect = 2.25e-292
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS
Sbjct: 452 NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 511

Query: 61  QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 120
           QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE
Sbjct: 512 QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 571

Query: 121 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 180
           EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI
Sbjct: 572 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 631

Query: 181 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 240
           SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY
Sbjct: 632 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 691

Query: 241 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 300
           FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA
Sbjct: 692 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 751

Query: 301 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 360
           GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK
Sbjct: 752 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 811

Query: 361 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL
Sbjct: 812 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 854

[Close]

BLAST of Cucsat.G7708 vs. NCBI nr
Match: XP_038875756.1 (nitrate reductase [NADH]-like [Benincasa hispida])

HSP 1 Score: 733 bits (1893), Expect = 2.21e-258
Identity = 358/407 (87.96%), Postives = 384/407 (94.35%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPT+AGN +GGW+EKDKP PT+ S S
Sbjct: 450 NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTLAGNQSGGWKEKDKPIPTNYS-S 509

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           Q  +NK+TTT    FSMSEVKTHNS ESAWIVV NHVYDCTPFLK HPGGADSILINAGT
Sbjct: 510 QPSNNKTTTTTTTTFSMSEVKTHNSLESAWIVVHNHVYDCTPFLKAHPGGADSILINAGT 569

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAIKML+DYKIGDLLILNDNKD TSSPK +T+HVAL+PREKIPVKLIS
Sbjct: 570 DCTEEFDAIHSDKAIKMLQDYKIGDLLILNDNKDDTSSPKPITKHVALVPREKIPVKLIS 629

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRF LSSEDQ LGLPVGKHIFLCAN+DGKLCMRAYTPSSAVDQ GYFDLV
Sbjct: 630 KISISHNTRIFRFSLSSEDQTLGLPVGKHIFLCANIDGKLCMRAYTPSSAVDQMGYFDLV 689

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VKIYFKN+HPDFPNGGLMSQYLDSLPL S+V++KGPLGHIEYMGRGNF V+GKP+ AKKL
Sbjct: 690 VKIYFKNIHPDFPNGGLMSQYLDSLPLSSIVDIKGPLGHIEYMGRGNFTVHGKPKFAKKL 749

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANR+EEDILLREEL+TWAKEN+RLKIW
Sbjct: 750 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEEDILLREELDTWAKENQRLKIW 809

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVK+GV+EGWPYSVGYIRE +LREHIPMGSP+TLALVCGPPPMIEL
Sbjct: 810 YVVKEGVKEGWPYSVGYIRECVLREHIPMGSPETLALVCGPPPMIEL 855

[Close]

BLAST of Cucsat.G7708 vs. NCBI nr
Match: XP_023551321.1 (nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694 bits (1791), Expect = 6.88e-243
Identity = 334/407 (82.06%), Postives = 372/407 (91.40%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ GN +GGW+EKDK NPTD   S
Sbjct: 452 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSS 511

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    F MSEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 512 KPTNDKNKTTAAKTFPMSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 571

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI MLEDY IGDLL  ND+++ TSS +AV QHVAL+P +KIPVKLIS
Sbjct: 572 DCTEEFDAIHSDKAINMLEDYMIGDLLTENDSEEKTSSSEAVEQHVALVPGKKIPVKLIS 631

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+DQ LGLPVGKHIFLCAN++GKLCMRAYTPSS+VD+ G+FDLV
Sbjct: 632 KISISHNTRIFRFGLSSKDQVLGLPVGKHIFLCANIEGKLCMRAYTPSSSVDEMGFFDLV 691

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKN+HPDFPNGGLMSQYLDSLPLGS V+VKGPLGHIEYMGRGNF V+GKP+ AKKL
Sbjct: 692 VKVYFKNMHPDFPNGGLMSQYLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKL 751

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDPEDETEM+VVYANR EEDIL+REEL+TWAKENERLKIW
Sbjct: 752 AMVAGGTGITPIYQVVQAILKDPEDETEMYVVYANRREEDILVREELDTWAKENERLKIW 811

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVKDGV+EGWPYSVGYI+E +LREHIPMGSP+TLALVCGPPPMIEL
Sbjct: 812 YVVKDGVKEGWPYSVGYIKESVLREHIPMGSPETLALVCGPPPMIEL 858

[Close]

BLAST of Cucsat.G7708 vs. NCBI nr
Match: XP_023551317.1 (nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694 bits (1791), Expect = 7.11e-243
Identity = 333/407 (81.82%), Postives = 374/407 (91.89%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ GN +GGW+EKDK NPTD   S
Sbjct: 453 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSS 512

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    F MSEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 513 KPTNDKNKTTAAKTFPMSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 572

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI MLEDY IGDLL  ND+++ TSS +AV QHVAL+P +KIPVKLIS
Sbjct: 573 DCTEEFDAIHSDKAINMLEDYMIGDLLTENDSEEKTSSSEAVEQHVALVPGKKIPVKLIS 632

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+DQ LGLPVGKHIFLCAN++GKLCMRAYTPSS+VD+ G+FDLV
Sbjct: 633 KISISHNTRIFRFGLSSKDQVLGLPVGKHIFLCANIEGKLCMRAYTPSSSVDEMGFFDLV 692

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKN+HPDFPNGGLMSQYLDSLPLGS+V+VKGPLGHIEY+GRGNF V+GKP+ AKKL
Sbjct: 693 VKVYFKNMHPDFPNGGLMSQYLDSLPLGSIVDVKGPLGHIEYLGRGNFTVHGKPKFAKKL 752

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDPEDETEM+VVYANR+EEDILLREEL+TWAKENERLKIW
Sbjct: 753 AMVAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEEDILLREELDTWAKENERLKIW 812

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVKDGV+EGWPYSVGYI+E +LREHIPMGSP++LALVCGPPPMIEL
Sbjct: 813 YVVKDGVKEGWPYSVGYIKESVLREHIPMGSPESLALVCGPPPMIEL 859

[Close]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL
Match: I0CCB0 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR3 PE=2 SV=1)

HSP 1 Score: 820 bits (2117), Expect = 1.05e-292
Identity = 403/403 (100.00%), Postives = 403/403 (100.00%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS
Sbjct: 451 NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 510

Query: 61  QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 120
           QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE
Sbjct: 511 QQKDNKSTTTFSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGTDCTE 570

Query: 121 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 180
           EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI
Sbjct: 571 EFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLISKTTI 630

Query: 181 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 240
           SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY
Sbjct: 631 SHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLVVKIY 690

Query: 241 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 300
           FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA
Sbjct: 691 FKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKLAMVA 750

Query: 301 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 360
           GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK
Sbjct: 751 GGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIWYVVK 810

Query: 361 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL
Sbjct: 811 DGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 853

[Close]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL
Match: A0A6J1FK45 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444944 PE=3 SV=1)

HSP 1 Score: 691 bits (1782), Expect = 7.68e-242
Identity = 335/407 (82.31%), Postives = 371/407 (91.15%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ GN +GGW+EKDK NPTD   S
Sbjct: 452 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSS 511

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    FSMSEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 512 KPTNDKNKTTASKTFSMSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 571

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI MLEDY IGDLL  NDN++ TSS +AV QHVAL+P +KIPVKLIS
Sbjct: 572 DCTEEFDAIHSDKAINMLEDYIIGDLLTENDNEEKTSSSEAVEQHVALVPGKKIPVKLIS 631

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+DQ LGLPVGKHIFLCA ++GKLCMRAYTPSS+VD+TG+FDLV
Sbjct: 632 KISISHNTRIFRFGLSSKDQVLGLPVGKHIFLCAIIEGKLCMRAYTPSSSVDETGFFDLV 691

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKNVHPDFPNGGLMSQYLDSLPLGS V+VKGPLGHIEYMGRGNF V+GKP+ AKKL
Sbjct: 692 VKVYFKNVHPDFPNGGLMSQYLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKL 751

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDPED+TEM+VVYANR+EEDILLREEL+TWAKENERLKIW
Sbjct: 752 AMVAGGTGITPIYQVVQAILKDPEDKTEMYVVYANRTEEDILLREELDTWAKENERLKIW 811

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVKDGV+EGWPYSVG+I E ILREHIP GSP+ LALVCGPPPMIEL
Sbjct: 812 YVVKDGVKEGWPYSVGHINESILREHIPTGSPECLALVCGPPPMIEL 858

[Close]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL
Match: A0A6J1FF98 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444943 PE=3 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 1.29e-240
Identity = 331/407 (81.33%), Postives = 372/407 (91.40%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ GN +GGW+EKDK NPTD   S
Sbjct: 453 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSS 512

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    FS SEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 513 KPTNDKNKTTASKTFSRSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 572

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI ML+DY IGDLL  NDN++ TSS +AV QHVAL+P +KIPVKLIS
Sbjct: 573 DCTEEFDAIHSDKAINMLQDYIIGDLLTENDNEEKTSSSEAVEQHVALVPGKKIPVKLIS 632

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+DQ LGLPVGKHIFLCA ++GKLCMRAYTPSS+VD+ G+FDLV
Sbjct: 633 KISISHNTRIFRFGLSSKDQVLGLPVGKHIFLCAIIEGKLCMRAYTPSSSVDEMGFFDLV 692

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKNVHPDFPNGGLMSQYLDSLPLGS V+VKGPLGHIEYMGRGNF V+GKP+ AKKL
Sbjct: 693 VKVYFKNVHPDFPNGGLMSQYLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKL 752

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDP+D+TEM+VVYANR+EEDILLREEL+TWAKENERLKIW
Sbjct: 753 AMVAGGTGITPIYQVVQAILKDPQDKTEMYVVYANRTEEDILLREELDTWAKENERLKIW 812

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVK+GV+EGWPYSVGYI+E +LREHIPMGSP++LALVCGPPPMIEL
Sbjct: 813 YVVKEGVKEGWPYSVGYIKESVLREHIPMGSPESLALVCGPPPMIEL 859

[Close]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL
Match: A0A6J1FFD6 (Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444945 PE=3 SV=1)

HSP 1 Score: 684 bits (1765), Expect = 2.88e-239
Identity = 330/407 (81.08%), Postives = 371/407 (91.15%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ G+ +GGW+ KDK NPTD   S
Sbjct: 452 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGHQSGGWKGKDKINPTDPPSS 511

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    FSMSEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 512 KPTNDKNKTTASKTFSMSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 571

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI MLEDY IGDLL  NDN++ TSS +AV QHVAL+P +KIPVKLIS
Sbjct: 572 DCTEEFDAIHSDKAINMLEDYIIGDLLTENDNEEKTSSSEAVEQHVALVPGKKIPVKLIS 631

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+DQ LGLPVGKHIFLCA ++GKLCMRAYTPSS+VD+  +FDLV
Sbjct: 632 KISISHNTRIFRFGLSSKDQVLGLPVGKHIFLCAIIEGKLCMRAYTPSSSVDEMSFFDLV 691

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKNVHPDFPNGGLMSQYLDSLPLGS V+VKGPLGHIEYMGRGNF V+GKP+ AKKL
Sbjct: 692 VKVYFKNVHPDFPNGGLMSQYLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGKPKFAKKL 751

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDP+D+TEM+VVYANR+EEDILLREEL+TWAKENERLKIW
Sbjct: 752 AMVAGGTGITPIYQVVQAILKDPQDKTEMYVVYANRTEEDILLREELDTWAKENERLKIW 811

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVVK+GV+EGWPYSVGYI+E +LREHIPMGSP++LALVCGPPPMIEL
Sbjct: 812 YVVKEGVKEGWPYSVGYIKESVLREHIPMGSPESLALVCGPPPMIEL 858

[Close]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL
Match: A0A6J1K1G0 (Nitrate reductase OS=Cucurbita maxima OX=3661 GN=LOC111489275 PE=3 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 2.48e-235
Identity = 324/407 (79.61%), Postives = 369/407 (90.66%), Query Frame = 0


Query: 1   NLNWNLMGMMNNSWYRIKINICKPQKGEIGIVFRHPTVAGNNAGGWQEKDKPNPTDESPS 60
           NLNWNLMGMMNNSW+RIKIN+CKPQ GE+GIVFRHPT+ GN +GGW+EKDK NPTD   S
Sbjct: 452 NLNWNLMGMMNNSWFRIKINVCKPQNGELGIVFRHPTLPGNQSGGWKEKDKINPTDPPSS 511

Query: 61  QQKDNKSTTT----FSMSEVKTHNSSESAWIVVQNHVYDCTPFLKDHPGGADSILINAGT 120
           +  ++K+ TT    FSMSEVKTHN+ ES+WIVV  HVYDCTPFLK HPGGADSILINAGT
Sbjct: 512 KPTNDKNRTTASKTFSMSEVKTHNTPESSWIVVHGHVYDCTPFLKAHPGGADSILINAGT 571

Query: 121 DCTEEFEAIHSDKAIKMLEDYKIGDLLILNDNKDATSSPKAVTQHVALIPREKIPVKLIS 180
           DCTEEF+AIHSDKAI MLEDY IGDLL  ND+++ TSS + V QHV L+P ++IPVKLIS
Sbjct: 572 DCTEEFDAIHSDKAINMLEDYIIGDLLTENDSEEKTSSSEPVEQHVELVPGKRIPVKLIS 631

Query: 181 KTTISHNTRIFRFGLSSEDQRLGLPVGKHIFLCANVDGKLCMRAYTPSSAVDQTGYFDLV 240
           K +ISHNTRIFRFGLSS+D+ LGLPVGKHIFLCAN++GK CMRAYTPSS+VD+ G+FDLV
Sbjct: 632 KISISHNTRIFRFGLSSKDRVLGLPVGKHIFLCANMEGKPCMRAYTPSSSVDEMGFFDLV 691

Query: 241 VKIYFKNVHPDFPNGGLMSQYLDSLPLGSMVEVKGPLGHIEYMGRGNFRVNGKPRLAKKL 300
           VK+YFKN+HPDFPNGGLMSQYLDSLPLGS V+VKGPLGHIEYMGRGNF V+G+P+ AKKL
Sbjct: 692 VKVYFKNMHPDFPNGGLMSQYLDSLPLGSTVDVKGPLGHIEYMGRGNFTVHGRPKFAKKL 751

Query: 301 AMVAGGTGITPIYQVVQAILKDPEDETEMFVVYANRSEEDILLREELETWAKENERLKIW 360
           AMVAGGTGITPIYQVVQAILKDPED+TEM+VVYANR+EEDILLREEL+ WA+ENERLKIW
Sbjct: 752 AMVAGGTGITPIYQVVQAILKDPEDKTEMYVVYANRTEEDILLREELDRWAEENERLKIW 811

Query: 361 YVVKDGVQEGWPYSVGYIREDILREHIPMGSPQTLALVCGPPPMIEL 403
           YVV +GV+EGWPYSVG+I+E ILREHIPMGSP++LALVCGPPPMIEL
Sbjct: 812 YVVNEGVKEGWPYSVGHIKESILREHIPMGSPESLALVCGPPPMIEL 858

The following BLAST results are available for this feature:

BLAST of Cucsat.G7708 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P39865	4.6e-166	65.71	Nitrate reductase [NADH] 1 OS=Phaseolus vulgaris OX=3885 GN=NIA1 PE=3 SV=1	[more]
P17569	2.5e-164	65.03	Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
P27783	3.7e-163	65.80	Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1	[more]
P39869	9.0e-162	64.45	Nitrate reductase [NADH] OS=Lotus japonicus OX=34305 GN=NIA PE=3 SV=1	[more]
P39870	1.2e-161	64.87	Inducible nitrate reductase [NADH] 2 OS=Glycine max OX=3847 GN=INR2 PE=2 SV=1	[more]

BLAST of Cucsat.G7708 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267688.1	2.17e-292	100.00	nitrate reductase [NAD(P)H]-like [Cucumis sativus] >AFH74423.1 cucumber nitrate ...	[more]
KAE8652087.1	2.25e-292	100.00	hypothetical protein Csa_018542 [Cucumis sativus]	[more]
XP_038875756.1	2.21e-258	87.96	nitrate reductase [NADH]-like [Benincasa hispida]	[more]
XP_023551321.1	6.88e-243	82.06	nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo]	[more]
XP_023551317.1	7.11e-243	81.82	nitrate reductase [NADH]-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G7708 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
I0CCB0	1.05e-292	100.00	Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR3 PE=2 SV=1	[more]
A0A6J1FK45	7.68e-242	82.31	Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444944 PE=3 SV=1	[more]
A0A6J1FF98	1.29e-240	81.33	Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444943 PE=3 SV=1	[more]
A0A6J1FFD6	2.88e-239	81.08	Nitrate reductase OS=Cucurbita moschata OX=3662 GN=LOC111444945 PE=3 SV=1	[more]
A0A6J1K1G0	2.48e-235	79.61	Nitrate reductase OS=Cucurbita maxima OX=3661 GN=LOC111489275 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 335..346 score: 64.41 coord: 297..316 score: 72.29 coord: 198..209 score: 75.0 coord: 219..226 score: 71.35 coord: 391..399 score: 75.0 coord: 264..278 score: 63.47
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 118..125 score: 66.67 coord: 130..142 score: 50.12 coord: 93..103 score: 69.56 coord: 103..117 score: 69.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 71..143 e-value: 2.6E-26 score: 103.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 73..142 e-value: 1.8E-22 score: 79.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 68..143 score: 26.586113
IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	PRINTS	PR00371	FPNCR	coord: 102..112 score: 37.1 coord: 391..399 score: 45.08 coord: 335..346 score: 46.55 coord: 367..383 score: 26.98 coord: 219..226 score: 54.6 coord: 251..260 score: 35.06 coord: 323..332 score: 37.36 coord: 297..316 score: 48.16
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 159..273 e-value: 3.1E-40 score: 138.8
None	No IPR available	GENE3D	2.60.40.650		coord: 1..68 e-value: 6.9E-12 score: 47.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..74
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..402
None	No IPR available	PANTHER	PTHR19370:SF198	NITRATE REDUCTASE	coord: 2..402
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 172..402 e-value: 1.73094E-102 score: 301.408
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 274..403 e-value: 1.2E-40 score: 140.7
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 267..402
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 172..278 e-value: 4.4E-36 score: 123.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 69..143 e-value: 8.4E-29 score: 101.5
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 67..142
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 298..402 e-value: 8.1E-33 score: 113.3
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 99..106
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 167..279 score: 14.530417
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 160..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G7708.T1	Cucsat.G7708.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0006809	nitric oxide biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0030151	molybdenum ion binding
molecular_function	GO:0043546	molybdopterin cofactor binding
molecular_function	GO:0009703	nitrate reductase (NADH) activity
molecular_function	GO:0050464	nitrate reductase (NADPH) activity
molecular_function	GO:0016491	oxidoreductase activity